CN109810984A - A kind of relevant Sesame SiGolS6 of drought resisting and its application - Google Patents

Abstract

The invention discloses a kind of Sesame SiGolS6 relevant to drought resisting and its applications.A kind of relevant sesame (Sesamum indicum L.) the gene SiGolS6 of drought resisting, nucleotides sequence is classified as shown in SEQ ID NO.1, or the nucleotide sequence with same function to be generated by the sequence addition, substitution, insertion or deletion one or more nucleotide.By the overexpression by sesame gene related to drought tolerance SiGolS6 in arabidopsis, it is found that this gene can significantly improve the drought resistance of arabidopsis, has preferable application prospect applied to crop drought resistance breeding.

Description

A kind of relevant Sesame SiGolS6 of drought resisting and its application

Technical field

The invention belongs to molecular biology fields, and in particular to a kind of Sesame SiGolS6 relevant to drought resisting, simultaneously Further relate to application of the gene in crop drought resistance breeding.

Background technique

Sesame (Sesamum indicum L.) is one of main oil crops in China, has the title of the hat of eight paddy, has Higher application value is distributed widely in the torrid zone and subtropical zone.The oil content of sesame seed is higher, and China just has from ancient times With the well-known allusion with sesame and sesame oil production delicious food.However as main sesame producing country in the world and Trading country, the self-supporting but wretched insufficiency in China, and also import rate also rises year by year.

In recent years, Sesame Seed Yield significantly decreases trend, and the more adverse circumstance side of body is subjected to when this is with sesame growth course Urgent influence is related, including the factors such as arid, damage or crop failure caused by waterlogging, with high salt.Since sesame is mainly planted in arid and semi-arid areas, It is highly prone to the influence of drought stress.Drought injury occurrence frequency gradually rises in recent years in China, China sesame main producing region, spring sowing area, For the sesame of autumn sowing area plantation throughout the year by drought impact, the underproduction is serious.In addition, in some sesame big producers in Africa, arid is same It is serious to sesame plantation industry harm.Therefore, it is excavated by reinforcing sesame drought resisting functional gene, high yield is steady under the conditions of obtaining drought injury The sesame variety of production is promoting the industry development of China's sesame, improves the self-sustaining aspect of total amount, very necessary.

The environmental factors such as arid, with high salt and high/low temperature are these stress an important factor for influencing plant normal growth development It will lead to the extensive underproduction of many crops.Therefore cultivating degeneration-resistant crop varieties is always agricultural cience and farming techniques research An important ring.Recent studies indicate that in order to resist or adapt to the unfavorable factors such as arid, plant can be with external environment Variation extraneous unfavorable signal is reached into the cell through a variety of ways, some response genes of inducing expression, and then generation Make cell from substances such as functional protein, the osmotic fields of the stress damages such as arid, with high salt, adaptation is made to extraneous variation Property reaction (Xiong etc., 2002).Plant can prevent moisture loss by osmotic adjustment, maintain cell turgor, guarantee plant Normal metabolic activity.Therefore, osmotic adjustment is considered as that plant adapts to one of important channels of environment stresses such as arid.

Gossypose series of oligosaccharides (RFOs) is a kind of small point of osmotic adjustment, is had in the different tissues of plant point Cloth, content is relatively high in higher plant seed.Research discovery gossypose series of oligosaccharides metabolism and plant growth in recent years Development, degeneration-resistant reaction (Wang Yi etc., 2018) and seed vitality build up (Li Tao etc., 2017) in close relations.Galactinol Enzyme (Galactinol synthase, GolS) participates in the first step (Dey etc., 1985) of catalysis RFO synthesis, and the step is RFO family The committed step (Saravitz etc., 1987) of each substance dynamic accumulation in race.Therefore, galactinol synthase is in Genes For Plant Tolerance Key player is play in inverse.It is reported that the overexpression of AtGolS2 significantly improves the drought-resistant ability (Taj of Arabidopsis plant Deng 2002).Hygrometric boea herb galactinol synthase gene (BhGolS1) is overexpressed in tobacco, significantly improves and turns base Because of the drought-resistant ability (Wang etc., 2009) of plant.In addition, corn ZmGOLS2 by DREB2A transcription factor regulate and control, ZmGOLS2 with Arabidopsis resistance can be improved in DREB2A overexpression, and to the no any negative effect of the growth of plant under normal operation (Gu etc., 2016).It can be seen that galactinol synthase has broad application prospects in crop drought resistance genetic improvement.

Sesame is one of important traditional oil crops in China, and the Sesame Cultivar for cultivating better resistance is significant.Therefore, Un-mixing bases are because and identifying the function that it is played in terms of drought resistance, will have for cultivating degeneration-resistant Sesame Cultivar from sesame There is very important meaning.

Summary of the invention

Technical problem to be solved by the invention is to provide a kind of relevant sesame of drought resisting (Sesamum indicum L.) Gene SiGolS6, coding albumen and application.

In order to solve the above technical problems, The technical solution adopted by the invention is as follows:

It provides a kind of drought resisting relevant sesame (Sesamum indicum L.) gene SiGolS6, there is such as SEQ ID The tool that nucleotide sequence shown in NO.1 or the sequence are added, replace, are inserted into or lack one or more nucleotide and generate There is the nucleotide sequence of same function.Pass through the overexpression by drought resisting correlation Sesame SiGolS6 in arabidopsis, discovery This gene is remarkably improved the drought-resistant ability of plant, provides genetic resources for plant drought breeding.

The invention also includes a kind of sesame SiGolS6 albumen is provided, there is the amino acid sequence as shown in SEQ ID NO.2 Column or the sequence are through replacement, missing or one or several amino acids formed amino acid sequences with same function of addition.

The present invention include obtain by various methods, the amino acid sequence as shown in SEQ ID NO.2 or the sequence be through replacing It changes, lack or adds one or several amino acids formed derived proteins with same function and encode these and derive egg The gene of white matter.

The invention also includes the cloning vector containing SiGolS6 nucleotide sequence or all kinds of expression vectors, contain the load The host cell of body.

By SiGolS6, gene constructed to expression vector pCAMBIA1301S, (pCAMBIA1301S is in the world to the present invention It is reconstructed on the basis of common Genetic Transformation in Higher Plants carrier pCAMBIA1301, carrying has composing type and overexpression feature The genetic transformation carrier of cauliflower mosaic virus CaMV35S promoter).It, will by Agrobacterium-medialed transformation method The SiGolS6 gene that pCAMBIA1301S is carried is transferred to arabidopsis, obtains transformation of Arabidopsis thaliana plant.The experimental results showed that SiGolS6 has the function of improving plant drought resistance.

The present invention also provides above-mentioned SiGolS6 gene, carrier or host cells to improve the application in plant drought resistance.

Compared with the prior art, the advantages of the present invention are as follows:

The present invention is to report drought resisting correlation Sesame SiGolS6 sequence for the first time both at home and abroad, its overexpression can mention at present The function of high plant drought resistance does not have relevant report, our experiments show that: the gene is remarkably improved plant drought ability, is applied to Crop drought resistance breeding.By overexpression of the SiGolS6 in arabidopsis, the expression of SiGolS6 and receptor in transgenic arabidopsis Compared to having significant improvement, therefore Arabidopsis plant drought-resistant ability is also improved for control (nontransgenic plants).Cause This present invention proposition can improve the drought-resistant ability of plant using the overexpression of SiGolS6 for use in oil crops drought resisting product Kind breeding, thus improve crop drought resistance, guarantee high crop yield stable yields.

Detailed description of the invention

Fig. 1 is expression of the SiGolS6 gene under a variety of adverse circumstances.Each processing sample are as follows: drought stress (15% The simulation of PEG6000 solution) processing 0h, 2h, 6h, 12h；High salt treatment 0h, 2h, 6h, 12h.

Fig. 2 is the building of the plant expression vector pCAMBIA1301S-SiGolS6 of the gene containing SiGolS6；

Fig. 3 pCAMBIA1301S-SiGolS6 convert Escherichia coli, the positive colony institute upgrading grain sequencing result of acquisition with SiGolS6 full length gene CDS sequence alignment schematic diagram (1:SiGolS6 full length gene CDS sequence；2: sequencing result)；

Fig. 4 turns SiGolS6 gene arabidopsis T1 for the selection result schematic diagram of positive plant；

Fig. 5 turns the PCR qualification result schematic diagram (M:Marker in SiGolS6 gene arabidopsis T1 generation；1-9: transgenosis T1 generation Plant；WT: wildtype Arabidopsis thaliana)；

Fig. 6 turns SiGolS6 gene arabidopsis T2 for the selection result schematic diagram of positive plant；

Fig. 7 turns the PCR qualification result schematic diagram (M:Marker in SiGolS6 gene arabidopsis T2 generation；1-6: transgenosis T2 generation Plant；WT: wildtype Arabidopsis thaliana)；

Fig. 8 turns SiGolS6 gene arabidopsis T2 for plant quantitative expression verification result figure；

The measurement of Fig. 9 antecedent soil moisture method turns SiGolS6 gene arabidopsis T2 for the drought resistance of plant.

Specific embodiment

Following embodiment defines the present invention, and describing the present invention in clone includes that SiGolS6 gene completely encodes The DNA fragmentation of section, and the method for verifying SiGolS6 gene function.

The method for cloning the relevant gene SiGolS6 of heretofore described drought resisting be in this field commonly used by method. The method of mRNA is extracted also there are many mature technology, kit (EASY spin Plus Plant RNA kit) can be from business Approach obtains (being purchased from Aidlab Biotechnologies Co., Ltd).It constructs heretofore described carrier and turns carrier Enter digestion used in plant, connection, inflorescence the methods of infect is also technology commonly used in the art.Plasmid involved in it (pCAMBIA1301), media (agrobacterium tumefaciens lba4404 and agents useful for same ingredient such as sucrose, Kan, hygromycin etc.) is used in transfection Commercially obtain.

The acquisition table of embodiment 1:SiGolS6 gene

It is compared within the scope of sesame full-length genome by homology sequence using the protein sequence of arabidopsis GolS gene Analysis, identification obtains 7 sesame GolS genes, including SiGolS6 gene.

By searching for Sesame group database (http://ocri-genomics.org/Sinbase/ Index.html), according to the CDS sequence of SiGolS6 gene, design can expand the primer of its complete CDS sequence.Take flowering stage Tissue after the drought stress 7 days of drought-resistant variety Shanxi sesame No. 2 (is purchased from EASYspin Plus plant RNA extraction kit Aidlab company), extract total serum IgE, using reverse transcriptase (be purchased from Vazyme company) by its reverse transcription synthesize cDNA (method according to Vazyme company reverse transcriptase reagent specification).Using the cDNA after reverse transcription as template, the primer title (includes with sequence Modified base) as follows:

SiGolS6FL-F:5 '-GGGGTACCATGGTTCCTGAAATTATCATTCC-3 ', as shown in SEQ ID NO.3；

SiGolS6FL-R:5 '-CGGGATCCTCAACCGGAAACTTGATCAATTG-3 ', as shown in SEQ ID NO.4；

Being gone out with above-mentioned primer amplification includes the cDNA segment of SiGol62 gene complete coding region, the segment that amplification is obtained It is sequenced, SiGolS6 gene order is obtained, as shown in SEQ ID NO.1.The amino acid sequence of protein encoded by it is such as Shown in SEQ ID No.2.

The expression of the endogenous SiGolS6 gene of 2 sesame of embodiment

Sesame drought stress transcriptome analysis shows that SiGolS6 is expressed by drought stress induced strong, may have raising The function of sesame drought resistance.In order to further confirming that transcript profile as a result, applicant's SiGolS6 gene endogenous to sesame is inverse Expression under the stress of border is detected.

Applicant selects sesame variety " middle sesame 13 " (disclosure that Inst. of Oil Crops, Chinese Academy of Agriculture cultivates The sesame variety of popularization and application) material as expression pattern analysis.It grows to 4 leaf phase seedling and carries out various adverse circumstance processing.Arid Processing is simulated with the processing of 15%PEG6000 solution, 0h, 2h, is sampled after 6h, 12h；High-salt stress is to be immersed in seedling root The solution of 200mM NaCl, 0h, 2h are sampled after 6h, 12h.The extraction of total serum IgE EASYspin Plus plant RNA extraction reagent Box (be purchased from Aidlab company), using reverse transcriptase (being purchased from Vazyme company) by its reverse transcription synthesize cDNA (method according to Vazyme company reverse transcriptase reagent specification).Using above-mentioned reverse transcription synthesis cDNA as template, with primer (SiGolS6-F: 5 '-CCAATCCCCGCAACCTATAAC-3 ', as shown in SEQ ID NO.5 and SiGolS6-R:5 '- TGCTCTTTGTCGGTGTACTTC-3 ', as shown in SEQ ID NO.6) special PCR amplification is carried out to SiGolS6 gene.Together When with primer (SiHF:5 '-GTTGGTCTCTTTGAGGAC-3 ', as shown in SEQ ID NO.7 and SiHR:5 '- CAGCTGGATGTCTTTTGG-3 ', as shown in SEQ ID NO.8) specific amplified is done to sesame Histone H3.3 gene, to make Quantitative analysis is carried out for internal reference.Reaction condition are as follows: 95 DEG C of 30sec；95 DEG C of 10sec, 60 DEG C of 30sec, 35 circulations.It reacted Fluorescence detection real-time quantitative analysis (qRT-PCR Mix: Nanjing Vazyme Biotechnology Co., Ltd. is carried out in journey；Instrument: Roche480).The result shows that SiGolS6 gene (SEQ NO:1) is in arid (15%PEG6000 solution processing Simulation) and high salt treatment after expression quantity rise (Fig. 1), illustrate SiGolS6 gene may enhance sesame drought resistance in have Important function.

A kind of embodiment 3: application of the Sesame SiGolS6 in plant drought breeding improving plant drought resistance

1) embodiment 2 is cloned into the obtained relevant Sesame SiGolS6 of drought resisting and (this laboratory pCAMBIA1301S There is provided) plasmid connection building plant expression vector, it is named as pCAMBIA1301S-SiGolS6 (Fig. 2), concrete operations are as follows:

1. target gene and carrier to be carried out to double digestion (BamHI and KpnI) (Takara method) respectively first, enzyme is obtained Product is cut, agarose gel electrophoresis is then utilized respectively and plastic recovery kit (Tiangeng biochemical technology Co., Ltd) is pure by its Change.

2. target fragment DNA and linearized vector are added in the centrifuge tube of 1.5ml with the molar ratio of 3:1 in T₄DNA connects It connects and is attached under enzyme (Takara) effect, sufficiently but after soft mixing pause to be centrifuged, be placed in 4 DEG C of reactions overnight.Draw 10 μ L Reaction solution to from taken out in -80 DEG C of refrigerators on ice melt 10min 50 μ L DH5a competent cells in, use pipettor It mixes gently, then at being incubated for 20min on ice, 42 DEG C are quickly set cooled on ice 2min after water-bath heat shock 45 seconds.

3. 300 μ L LB liquid mediums are added in clean bench, 37 DEG C of incubation 60min.13,000rpm is centrifuged 1min and collects Thallus abandons part supernatant, thallus is resuspended with remaining culture medium, is trained with sterile spreading rod in the LB solid containing Kan resistance It supports and is gently smoothened on base, culture 16-24h is inverted in 37 DEG C of incubators.

4. the clone of several recombining reactions of picking conversion carries out bacterium colony PCR identification, it will be accredited as each of the positive later Single colonie picks them separately in the LB liquid medium containing Kan antibiotic 37 DEG C, is incubated overnight in 200rpm incubator, mentions Take plasmid, and corresponding bacterium solution direct Sequencing is identified into carrier accuracy.By sequence alignment (Fig. 3), sequencing result with SiGolS6 full length gene CDS sequence is completely the same, illustrates that the plant expression vector of building is accurate.

2) the carrier pCAMBIA1301S-SiGolS6 prepared in step 1) is transferred to agrobacterium tumefaciens lba4404 (Shanghai Wei Di Bioisystech Co., Ltd), then import in Arabidopsis plant, concrete operations are as follows:

(1) recombinant vector is transferred to Agrobacterium LBA4404:

1. 2 μ g Plasmid DNA are added in every 100 μ L LBA4404 Agrobacterium competent cell, tube bottom is dialed with hand and is mixed, according to Inferior to standing 5min, liquid nitrogen 5min, 37 DEG C of water-bath 5min, ice bath 5min on ice.

2. the LB liquid medium of 700 μ L antibiotic-frees is added, in 28 DEG C of shaken cultivation 5h.13,000rpm centrifugation 1min Thallus is collected, 100 μ L or so supernatant is left and taken, gently thallus is resuspended in piping and druming, it is equably coated on to the LB containing Kan and Rif On solid medium, 28 DEG C culture carton upside down culture 2 days, primer of several positive colonies of picking in embodiment 2 SiGolS6FL-F/R, which carries out bacterium colony PCR, can amplify target fragment, illustrate that carrier pCAMBIA1301S-SiGolS6 has been transferred to In agrobacterium tumefaciens lba4404.

(2) the plate culture of arabidopsis:

1. needing to count a certain number of arabidopsis seeds loaded in sterile 1.5mL centrifuge tube according to experiment.

2. 75% ethyl alcohol of 1mL is added, mixing of turning upside down is abandoned supernatant, is repeated 1 times.It is put into 37 DEG C of 200rpm shaking tables and shakes 10min surface sterilizing.

3. abandoning 75% ethyl alcohol, 95% ethyl alcohol of 1mL is added, mixing of turning upside down is abandoned supernatant, and is repeated 1 times.In ultra-clean work Make in platform, 300-500 μ L dehydrated alcohol is added in Xiang Geguan seed, is sprayed onto seed and dehydrated alcohol together with 1mL pipettor On aseptic filter paper.

4. being evaporated completely to ethyl alcohol, the arabidopsis seed point done is sowed to the plate of ready MS culture medium with toothpick On.

5. plate is sealed, it is inverted in 4 DEG C of vernalization 48h under dark condition, after vernalization, plate is placed in illumination cultivation It is cultivated vertically in case, emergence can transplant after a week.

6. in the soil that seedling is planted to small basin with tweezers, first using preservative film moisturizing 48h, middle culture is straight between being placed in plant growth When growing bolting to arabidopsis (about one month), it to be used for transformation experiment.

(3) genetic transformation:

1. Agrobacterium activates: it is separately added into 20 μ L Rif and Kan in the LB liquid medium of 20mL, shakes up and connect bacterium, 28 DEG C of 220rpm concussion activation 8-10h.

2. Agrobacterium expands culture: being separately added into 200 μ L Rif and Kan in the LB liquid medium of 200mL, add The activation bacterium solution of 5-10mL, 28 DEG C of 220rpm shake culture 14-16h, until OD value 1.6-2.0,4500rpm are centrifuged 10min, precipitating Thallus abandons supernatant, naturally dry.

3. 5% sucrose solution of 100mL is added in precipitating thallus is resuspended thallus, pipettor piping and druming is uniform, thallus is resuspended.

4. bacterium solution in centrifugal bottle is added in plate, 5% sucrose solution of 100mL is added, 40 μ L are added before converting Silwet-L-77 (0.02%) shakes plate and mixes.

5. arabidopsis floral is closed up, plate is immersed, 15s is shaked gently.

6. plant is packaged with black sack, be protected from light moisturizing for 24 hours.

7. it is primary to repeat conversion after a week.

3) screening T1 is for positive plant.

The seed for harvesting arabidopsis T0 generation, is sterilized, and the MS screening and culturing medium (addition of the hygromycin containing 30mg/L is inoculated with 25mg/L cephalosporin is antibacterial) it is upper 22 DEG C illumination cultivation 7-10 days, screening obtain positive plant (seedling and root system all normal growths Plant) (Fig. 4), this experiment obtains 6 positive strains, by positive transplantation of seedlings into soil, with taking off behind on preservative film cover 2-3 days Film, then normal growth.After the leaf DNA of the positive plant filtered out is extracted, SiGolS6 gene is identified using PCR method, into The Molecular (Fig. 5) of the target gene of row transgenic plant, it is final to confirm that gene has been transferred to T1 for positive plant.

4) transgenic plant T2 is for positive detection

T1 carries out single plant sowing for positive plant, and the T1 seed harvested for positive plant is continued hygromycin selection and is obtained T2 for positive plant (plant of seedling and root system all normal growths) (Fig. 6), choose above-mentioned acquisition part positive strain system by its Transplanting growth extracts leaves genomic DNA and carries out PCR Molecular Identification (Fig. 7), determines T2 for positive plant.

5) transgenic plant T2 is verified for positive plant quantitative expression

Transgenic plant T2 is taken to be mentioned for the young leaflet tablet of positive plant and wild-type Arabidopsis plants growth period with RNA Kit (Beijing Ai Delai Biotechnology Co., Ltd) is taken to extract blade total serum IgE, then (Nanjing promise is only with reverse transcription reagent box Praise Biotechnology Co., Ltd) cDNA is obtained, using respective cDNA as template, using arabidopsis β-actin as internal reference (aF:5 '- CCCGCTATGTATGTCGCCA-3 ', as shown in SEQ ID NO.9；AR:5 '-AACCCTCGTAGATTGGCACAG-3 ', such as SEQ Shown in ID NO.10), target gene quantifies primer sequence are as follows: SiGolS6-F:5 '-CCAATCCCCGCAACCTATAAC-3 ', such as It is carried out as shown in SEQ ID NO.6 shown in SEQ ID NO.5 with SiGolS6-R:5 '-TGCTCTTTGTCGGTGTACTTC-3 ' QRT-PCR expression verifying (qRT-PCR Mix: Nanjing Vazyme Biotechnology Co., Ltd.；Instrument: Roche 480).The result shows that being control, 3 arabidopsis of the sesame SiGolS6 gene in detection with the wildtype Arabidopsis thaliana of non-transgenosis Expression quantity significantly improves in the blade of transgenic line, and in the blade of non-non-transgenic control arabidopsis, do not detect sesame The expression (Fig. 8) of numb SiGolS6 gene.Show that the SiGolS6 gene of sesame converts and is inserted into corresponding arabidopsis gene In group and expressed.

6) T2 generation positive arabidopsis thaliana drought resistance measurement

It is wild to arabidopsis transgenic plant and non-transgenosis respectively to T2 for growth of transgenic plants to 3 pairs of leaf periods Type Arabidopsis plant carries out drought stress and handles 16 days, and discovery transgenic plant is withered with non-transgenosis wild-type Arabidopsis plants It is listless serious.After above-mentioned material is carried out rehydration processing 3 days, the results showed that compared with wildtype Arabidopsis thaliana control, this research institute is obtained The SiGolS6 gene arabidopsis strain that turns obtained is gradually brought to normal growing conditions, and wildtype Arabidopsis thaliana is then still in wilting State illustrates that turning SiGolS6 gene arabidopsis strain transgenic line drought-resistant ability compares (Fig. 9) higher than wildtype Arabidopsis thaliana, Show that the drought-resistant ability of plant can be improved in the overexpression of sesame SiGolS6 gene.

SEQUENCE LISTING

<110>Inst. of Oil Crops, Chinese Academy of Agriculture

<120>a kind of relevant Sesame SiGolS6 of drought resisting and its application

<160> 10

<170> PatentIn version 3.5

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<213> Sesamum indicum

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atggttcctg aaattatcat tccaacaata aaaaatgcag ctgaggcagc aagaactctt 60

ggagggaaca atgattgtag ctgtgcttat gttacgttct tggctggtga tggcgactac 120

gtgaaaggtg tgatttgttt ggcaaaagga ctaaggaagg tgaaagctgc ttatccgctg 180

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tgtctccttc gtgagattga gcccgtttgt cctcctgttg gcaacacgga tagccagtat 300

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ccttgtgcag acaaggtcca gtggcctgca gaactgggca atgaaccacc attttatttc 540

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aatacatcga ggccaatccc cgcaacctat aacttgcttg tggcaatgct ttggcggcac 720

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Asp Ile Gln Val Phe Asp Asn Ile Asp His Leu Phe Asp Leu Pro Ser

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Gly Cys Leu Tyr Ala Val Met Asp Cys Leu Cys Glu Met Gln Gly Lys

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Pro Phe Tyr Phe Asn Gly Gly Met Phe Val Phe Glu Pro Asp Leu Asp

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Claims (7)

1. a kind of relevant Sesame SiGolS6 of drought resisting, it is characterised in that: it has the nucleosides as shown in SEQ ID NO.1 Acid sequence or the sequence addition, substitution, insertion or deletion one or more nucleotide and the nucleosides with same function generated Acid sequence.

2. the sesame SiGolS6 albumen of Sesame SiGolS6 coding described in claim 1, it is characterised in that: it has such as Amino acid sequence shown in SEQ ID NO.2 or the sequence are through replacement, missing or one or several amino acids formed tools of addition There is the amino acid sequence of same function.

3. containing the cloning vector or all kinds of expression vectors of Sesame SiGolS6 nucleotide sequence described in claim 1.

4. the host cell containing carrier described in claim 3.

5. Sesame SiGolS6 described in claim 1 is in the application for improving plant drought resistance.

6. cloning vector as claimed in claim 3 or all kinds of expression vectors are improving the application in plant drought resistance.

7. host cell as claimed in claim 4 is improving the application in plant drought resistance.