CN109805259A - A kind of Mai Fupi powder or okara powder generate the preparation method of the fiber diet of aminobutyric acid containing y- - Google Patents

A kind of Mai Fupi powder or okara powder generate the preparation method of the fiber diet of aminobutyric acid containing y- Download PDF

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CN109805259A
CN109805259A CN201711245271.3A CN201711245271A CN109805259A CN 109805259 A CN109805259 A CN 109805259A CN 201711245271 A CN201711245271 A CN 201711245271A CN 109805259 A CN109805259 A CN 109805259A
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powder
enzyme
beans
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dregs
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王文华
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Abstract

A kind of Mai Fupi powder or okara powder generate the preparation method of the mono- aminobutyric acid fiber diet containing y, and the present invention is using biological enzymolysis function or fermentation process, and processing generates the Mai Fupi powder or okara powder and its foods with high dietary fiber of high-content y- aminobutyric acid;To rapid recovery fatigue, energy is supplemented, beneficial health benefit can be generated by reducing hypertension and the senile brain nerve disease of prevention encephalatrophy and improving sleep quality;Preferably solving makes its small molecule and the technology barriers such as finer and smoother.

Description

A kind of Mai Fupi powder or okara powder generate the preparation of the fiber diet of aminobutyric acid containing y- Method
Technical field
System of the present invention utilizes its biological enzymolysis function of the complex enzyme containing protease or fermentation process, and processing generates high-content y- The preparation method of the food such as the Mai Fupi powder or okara powder of aminobutyric acid and its high dietary-fiber.
Technical background
Y- aminobutyric acid English is known as: gaminobutyficacid. abbreviation: GABA, chemical name: 4-Aminobutanoicacid Alias: chromium propylhomoserin etc.;Molecular formula C4H9N02, molecular weight 103.1.It is a kind of naturally occurring functional amino, is that lactation is dynamic Important inhibiting nerve conveys substance in object central nervous system.It is present in Cerebral cortex hippocampus thalamus substrate mind in human body In warp knuckle and cerebellum, and play important inhibiting effect;There is adjustment effect to the multiple functions of human organism: to preventing hypertension Rise, promote brain metabolism and reactivity, improves cerebrovascular condition, and by outer injury remission and restored by head, And muscular dystrophy has significant effect.When human body lacks GABA, the tired worry of anxious and insomnia can be generated Equal unhealthy emotions and symptom, especially high amount of exercise crowd brain worker and 55 years old or more elderly population, are replenished in time appropriate GABA supplements energy to rapid recovery fatigue, reduce hypertension and the senile brain nerve disease of prevention encephalatrophy and Beneficial health benefit can be generated by improving sleep quality;In recent years it has also been found that have promote kidney Fitness improvement have protective effect; There are also inhibit fatty liver, activation liver function and body fat, beauty moisturizing function etc. [seeing internet].
The dietary fibers such as wheat husband powder, soybean peel and soybean residue are a treasured to health in human body intestinal canal.Because it is rich in solvable Property dietary fiber insoluble crude fibre, pectic substance lignin [soyabean oligosaccharides] hardly decomposed by the digested enzyme of human body, and Can water swelling in vivo, can be hydrated emulsification enteron aisle inner lipid;Especially it can be with water absorption rate up to 85% or so and tool There is the absorption endotoxic characteristic of human body intestinal canal, then intake person can be made to prevent heart disease diabetes colorectal cancer diverticulum disease gallbladder knot The ciril diseases such as stone and constipation;And pH value in adjustable enteron aisle, inhibit the generation of pathogen development breeding and putrefaction, increases stomach and intestine Wriggling and satiety, make bile acid transform into new substance -- deoxycholic acid lithocholic acid etc.;Make to cause prominent foreign substances with having been formed Enteron aisle is discharged in the water-soluble sample crude fibre of Gao nian degree gel state [referring to excrement];
In reality because of the dietary fibers staple foods such as the powder of husband containing wheat, bean dregs and food in reality because its is coarse, poor taste, fermentation Afterwards food quality it is undesirable and can not practical application;And this aminobutyric acid function of y- containing high-content and wheat husband powder, the bean dregs of effect Based on dietary fiber make its small molecule and finer and smoother etc. since application enzyme hydrolysis or fermentation technique technique preferably solve Technology barrier.
American Cancer Society recommends thick 25-30 grams of dietary fiber amount of adult day for human beings intake;Japanese recommends daily per capita 20 Gram or more;Chinese Soclety of Nutrition then recommends intake appropriate, and referring to is advisable with the smooth discharge stool of energy, about thick for 20-30 grams daily Fiber;See that<soy food product technology>Shi Yan state writes,<Chinese soybean food>Xu Minggao Liu Qipei text
Soluble dietary fiber refers to Mai Fupi, in soybean oligosaccharide, stachyose raffinose rhamnose etc.-be referred to as flatulence because Son, but because of enzymatic hydrolysis and fermentation in this technique, so being purified or weakening;Day takes in 2-3 grams in human body, then can make enteron aisle Interior Bifidobacterium is largely proliferated, and helps to balance and improve environment in human body intestinal canal;See < novel bean product processing technology with match Therefore side > Zhang Zhijian writes the prebiotic source of beneficial bacterium in referred to as human body;It is with beneficial bacteriums such as Bifidobacteriums in the initial enteron aisle of life To be most, gradually reduced after between twenty and fifty, and content is minimum after old age, thus Bifidobacterium etc. is beneficial to the how many change of bacterial content Change process indicates the process of human health aging;So modern medicine circle nutrition circle personage is finding energy by fair means or foul Increase effective ways -- the prebiotic source that Bifidobacterium can be proliferated in human body, health is increased with the anti-oxidant of anti-aging!And Soybean stachyose raffinose rhamnose etc. is the prebiotic source of best most effective Bifidobacterium again;And amount in soybean slag At most![Chinese soybean food net soy food product technology]
There is 60 years old or more population more than 200,000,000 in China, wherein about 20% or more suffers from constipation and hyperlipidemia to some extent Hyperglycemia coronary heart disease etc. ' rich people's disease ';Nearly 300,000,000 human body is overweight and obesity, they need to increase health from diet, Slow down and prevent chronic disease, thus, this is a huge market.
Since the development and application of dietary fiber function and product to high-content y- aminobutyric acid just start, perhaps More health mechanism and function need to be proved to be confirmed by science and in application.
The complex enzyme for being with sufu fermentation enzyme or dregs of beans hydrolase or soy sauce brewing enzyme and its with protease is therein One or two or more kinds of commercial enzymes.Albumen enzyme system caused by traditional single culture is difficult to fill in soybean, Mai Fuzhong protein Point catalysis dissociation, the enzyme kind application also have remove its bitter taste, increase delicate flavour material content, obtain taste and flavor It significantly improves;And have in the protein such as soybean and soybean powder amino acid carry out enzymatic hydrolysis at small molecule product, its The features such as time of fermentation process shortens.
The chemical nature of enzyme is the protein with catalysis effect.Since protease has the function of aminosal Can, the common addition of a variety of enzymes contained and protease can not be applied under normal circumstances;So enzyme preparation manufacturer will contain There is protease to compound with a variety of enzymes to release as the new commodity enzyme that can be used in conjunction with, be provided convenience for the application of its market And innovation.
In the catalyzing hydrolysis effect of enzyme or fermentation process, it can generate to protein, crude fibre in Mai Fupi powder or bean dregs Macromolecular in is decomposed into the small molecules state such as amino acid and oligosaccharide, cellulose;And using to amino in its protein The catalytic action for the glutamate decarboxylase that acid-is especially passed through with the enzymatic hydrolysis and fermentation and its product of glutamic acid or glutamine in the process Under make its conversion, thus generate the Mai Fupi powder for obtaining high-content y- aminobutyric acid, okara powder or its food etc..Its y- amino The content of butyric acid can 30-100mg or more or the high dietary-fiber class that can achieve 300mg/100g [dry meter] food or Other products.
Summary of the invention
By wheat husband powder or 100 jin of okara powder addition or 0.3 times of its volume -- 6 times of water, temperature within 100 degree of celsius, Within duration is kept for 1 hour;Be cooled to 30-60 degrees celsius or so or when, addition be mainly sufu fermentation enzyme or dregs of beans hydrolysis Enzyme or soy sauce brewing enzyme are [as the producers such as the Guangxi road Dong Henghua biotechnology company or Novozymes Company (novozymes) are given birth to Production and marketing is sold] it can be to the macromolecular complex such as protein peptide chain residue contained by the wheat husband powder, okara powder or matrix object catalysis enzymatic hydrolysis One or two or more kinds of complex enzymes containing protease of separation (are made of) product, agent etc. a variety of enzymes, can make its effect Substrate generate the amino acid-especially glutamic acid for turning more, and so that its glutamine is generated transfer, decarboxylation by the decarboxylase of institute's association The effects of, or make its decarboxylation using the material containing glutamic acid and glutaminase decarboxylase of external addition, and be conducive to more generate Y- aminobutyric acid ingredient;Thus it converts and itself is produced after being generated as the food than fermented production such as its wheat husband powder, okara powders The raw more y- aminobutyric acid ingredients of content.
By hydrates plastids such as the wheat husband powder of the ingredient of aminobutyric acid containing y-, okara powders, dry through dehydration or drying is through adding Work is made for all types of commodity;Or become the commodity or raw material of slurry class state etc. after the techniques such as crushing or attrition process again.
The wheat husband powder or okara powder processing after be mainly dietary fiber its dry matter in, every 100 grams can contain 15- 30 grams or more protein, y- aminobutyric acid have 50-100mg or more content [dry meter] -/100 grams;If by the wet shape wheat husband Powder or okara powder object are added directly into wheat flour or other coarse cereal powders, through zymotechnique again or direct machine-shaping and are cured into For food, the wheat husband powder or bean dregs foodstuff processing containing y- aminobutyric acid can be produced, as: Mian packet steamed bun ship biscuit energy stick, The health food or product of the different shapes such as piece, capsule and pill;If added so that dry matter is ground after the wheat husband powder or okara powder processing Work becomes the preparations such as pulvis or ball, piece, ball, capsule;Or utilize it as raw-food material, auxiliary material application;It can also be ground It is processed into liquid commodity product etc.;Because then at least containing 30mg or more in 100 grams of its y- aminobutyric acid;Mai Fupi powder, bean dregs Protein content about 14-30% in powder, aminoacid ingredient constitute in You Yiqi content of glutamic acid it is most.This contains wheat husband powder or beans The Mian food of the y- aminobutyric acid [GABA] of ground-slag and other food taste and flavors are good, easy to process, are easy to use, raw material Hand richness, lower-price characteristic;It is applied conducive to large-scale processing and food factory.
System of the present invention makes y- aminobutyric acid [GABA] energy stood high above the masses in the past using the technique of enzymatic hydrolysis catalysis and its fermentation Product in the form of full diet fiber preparation or food product or Mian powder etc. and others enters daily life or staple food In, health is brought for ruck and is lived up,
In the method for the invention, wheat husband powder or okara powder are dissociated or is fermented using the catalysis in the activated centre of mixed enzyme In the enzyme that produces of metabolism catalytic action is generated to all macromolecular complex activated centres such as protein in wheat husband powder or okara powder; The enzymatic hydrolysis can generate hydrolysis function effect to soybean meal component, such as protein, fat, soybean carbohydrate and its carbohydrate;Its Synergistic effect can be such that the quality of product is markedly superior to by the hydrolysis result of single protease or lipase or zytase etc.. Thus the glutamic acid in its amino acid is decomposited in its protein can also be dissociated catalysis;It all can be simultaneously according to each enzyme There are the theories that simultaneously association goes out same decarboxylase: in the present invention, through the soybean protein base for keeping long-time enzymatic hydrolysis and fermentation The y- amide groups of the glutamine residue in protein peptide chain can be generated in group or because makes the hair of glutamine and sodium glutamate Ferment can produce glutamine, while the glutamate decarboxylase of institute's association can carry out decarboxylation or the paddy ammonia through in addition adding to it Acid decarboxylase decarboxylation, thus it is converted into y- aminobutyric acid: it generates expressed by its chemical equation H2NcH2cH2cH2cocH A kind of chemical substance.
Embodiment,
1, wheat husband powder or okara powder or two kinds of mixed powder are predominantly added up to 100 jin, added within 6 times of its volume Water adjusts between pH value 3 one 10, adds predominantly sufu fermentation enzyme or dregs of beans hydrolase or soy sauce brewing enzyme or containing protease The enzyme of one of complex enzyme or two kinds or more, 0.002-1.5 jins of usage amount;The substrate for acting on it generates amino acid, conversion It is generated as raw material of the ingredient of aminobutyric acid containing y- as food;
2, predominantly by wheat husband powder or okara powder or more than 2 kinds of powder add up to 100 jin, add the water within 6 times of its volume, Or miscellaneous bacteria is killed to raw material;It when below 60 degrees celsius, adjusts between pH value 3 one 10, being added is mainly sufu fermentation enzyme or beans Dregs of rice hydrolase or soy sauce brewing enzyme or the complex enzyme containing protease etc. are one such or two kinds or more or can form fermentation 0.002-1.5 jins of enzyme;Or separately it is added with the material containing glutamic acid;Keep enzymatic hydrolysis or within yeastiness 50 hours;Or removal is more Remaining moisture becomes commodity powder or other products through dry;Or it is ground again or be crushed into liquid such as pulpous state class commodity etc.;Or it will Upright connect of the liquid material containing powder inserts in the production and processings of flour-made foods such as Mian packet steamed bun;Or dehydration be made for the powder of husband containing wheat, Okara powder food or various forms commodity;Processing or be and in wheat flour or rice meal or coarse cereal powder it is a kind of or two The Hun He Mian powder of the y- aminobutyric acid of kind ingredient;
3, it is mainly 100 jin of particle of wheat husband powder or wheat, adds 0.3 times of its volume -- 6 times of water, until about 60 degree or less When adjust pH value between 3-10;Be added is mainly sufu fermentation enzyme or sufu fermentation enzyme bacterium and dregs of beans hydrolase or protease Based on 0.002-1.5 jins of complex enzyme;Keep enzymatic hydrolysis or yeastiness;Become soya-bean milk shape liquid commodity after grinding again, or passes through again It is processed into other food goods of various forms;
It 4, is mainly bean curd ground-slag or 100 jin of soybean ground-slag or soybean skin powder, addition or 0.3 times of its volume -- 6 times of water, It is killed miscellaneous bacteria or does not kill miscellaneous bacteria, pH value is adjusted when within 60 degrees celsius of celsius between 3-10, being added is mainly fermented bean curd One such or two kinds or more the enzymes such as the complex enzyme based on fermenting enzyme or dregs of beans hydrolase or soy sauce brewing enzyme or protease 0.002-1.5 jins are added up to, and within keeping temperature 50 hours;
5, it is mainly that wheat husband powder adds 100 jin of skin of beancurd powder of okara powder or increasing, adds or 0.3 times of its volume -- 6 times of water goes out Kill miscellaneous bacteria or do not kill miscellaneous bacteria, when being kept for 60 degree of temperature or less, be added between adjustment pH value 3-9,5 be mainly sufu fermentation enzyme or Complex enzyme based on dregs of beans hydrolase or sufu fermentation enzyme or protease etc. is one of or 0.02-1.5 jins of enzyme of two kinds or more, Keep temperature to 4 hours or more after mixing evenly;Then ground it can become soya-bean milk shape liquid commodity,;Or through artificial drying side It is reprocessed into after method as raw material or other commodity;
6, be mainly wheat husband powder or add 100 jin of fresh bean dreg, addition or 0.5 times of its volume -- 6 times of water, kill miscellaneous bacteria or Miscellaneous bacteria is not killed, keeps adjusting between pH value 3-9,5 at 60 degree of temperature or less, sufu fermentation enzyme or dregs of beans hydrolase or corruption is added Complex enzyme based on milk fermentation enzyme or protease etc. is one of or 0.02-1.5 jins of enzyme of two kinds or more, keeps after mixing evenly Temperature was to 10 hours or more;Then ground it can become paste liquid;Or it is reprocessed into as raw material or other commodity;
7, it is technically characterized in that predominantly sufu fermentation enzyme or dregs of beans hydrolase or sufu fermentation enzyme or containing protease One of various complex enzymes or two kinds or more enzymes, be by plant produced be made sale or by market sale or containing hair The general designation of the commodity complex enzyme of yeast-like fungi;Or it can also be by the enzymes such as protease, cellulose fermentoid, zytase or zymophyte by market Buying is applied after voluntarily preparing.
In technical solution of the present invention application include add okara powder or fresh bean dreg using sufu fermentation enzyme in wheat husband's powder or When the one or two or more kinds of compound (matching) enzyme based on dregs of beans hydrolase or sufu fermentation enzyme or protease, in enzymolysis process Length reach 10 hours or more, add simultaneously the sufu fermentation enzyme or dregs of beans hydrolase or sufu fermentation enzyme one kind or two kinds with On content it is total when being greater than 0,1% or more, traditional ' fermentation ' or anaerobic fermentation can be generated, generate product can Such as: rouge, alcohol, aldehyde, ketone and oxidation material, as turned sour, in micro-perfume color, shape, the variation of the sense of taste or the secondary substance of generation etc. Phenomenon;
Beans seed through high-temperature steam or extruding and puffing technology or can also be equivalent to the technology, instantaneous deodorant flavouring Mode working process;So that the seed of big beans or cereal is produced apparent fragrance, is produced using crushing as the inventive method Bear the raw material for standby of the aminobutyric acid liquid material of y- containing high-content;
Substance caused by wheat husband powder, okara powder, fresh bean dreg and its beans paste liquid etc., or can be improvement mouthfeel wind Taste, improving yield and quality, added acid, alkali, salty, fragrant, sweet tea are in that taste and vitamin and mineral etc. meet < food addition again Allow the substance added in agent health mark Huaihe River > regulation;
Its protease is flavor protease, endo protease or exoproteinase or other compound protease;Cellulose Fermentoid, xylan fermentoid etc. are that can generate the enzyme of hydrolysis to lignin, cellulose etc. or are zymophyte;Or it is adopted by market Purchase is applied after voluntarily preparing;Or be by plant produced and sell commercial enzyme;
Zymophyte refer to can make wheat husband powder, okara powder generate fermentation yeast class, der Pilz, rice song class, Mucor class it is beneficial Bacterium etc..
Wheat husband's powder technical characteristic in the present invention, refers to and contains wheat, barley, oat, highland barley, naked oats, buckwheat class etc. The general designation of the substance after starch is artificially processed after crushing or extracted to high-cellulose wheat cereal;
Okara powder, which refers to, to be included soya bean belonging to soybean, semen sojae atricolor, green soya bean, sepia beans or paint face's beans, peanut meal powder and contains Powdery after the processed removal soya-bean milk of the bean varieties grain reality seed of the miscellaneous beans of the big grain such as red bean, mung bean, Peas, chick-pea, Particulate material and its soya-bean cake, dregs of rice powder [peanut dregs powder] are the general designation containing cellulosic material after processed crushing;
Fresh bean dreg includes that the bean varieties grain of soya bean belonging to big beans, semen sojae atricolor, green soya bean, sepia beans or paint face's beans is real It is after seed, and its processing removal soya-bean milk or containing after artificial addition water wet shape granular substance and its beans it is processed after be in scoriform The general name of substance;
Material containing glutamic acid means glutamic acid product or other manufactured goods rich in glutamic acid ingredient.
Embodiment:
1. predominantly by wheat husband powder or okara powder or more than 2 kinds of powder add up to 100 jin, add the water within 6 times of its volume, Or miscellaneous bacteria is killed to raw material;It when below 60 degrees celsius, adjusts between pH value 3 one 10, being added is mainly sufu fermentation enzyme or beans Compound (matching) enzyme based on dregs of rice hydrolase or soy sauce brewing enzyme or protease etc. is one such or two kinds or more or can be with shape At 0.2-1.5 jins of enzyme of fermentation;Or it is separately added with the material containing glutamic acid, keep enzymatic hydrolysis or within yeastiness 48 hours;Or Removing excessive moisture becomes commodity powder or other products through drying means;Or it is ground again or be crushed into liquid such as pulpous state class quotient Product etc.;Or upright connect of the liquid material containing powder is inserted in the production and processing of the flour-made foods such as Mian packet steamed bun;Or dehydration is made for The commodity of the food or various forms of the powder of husband containing wheat or okara powder;Processing or be and wheat flour or rice meal or coarse cereal powder The Hun He Mian powder of middle a kind of or two kinds of ingredients y- aminobutyric acid.During the Mai Fupi powder, okara powder powder [dry meter] are 100 grams every Its y- aminobutyric acid at least content is in 30-100mg or more;
2, soybean cake powder or bean curd ground-slag or soybean cake powder add 1 00 jin of bean curd ground-slag, 0.5 times of addition or its volume -- and 6 Times water, killed miscellaneous bacteria or do not kill miscellaneous bacteria, when maintaining the temperature within 60 degrees celsius of celsius adjust pH value 3-8,5 it Between, be added dregs of beans hydrolase add it is compound based on sufu fermentation enzyme or dregs of beans hydrolase or soy sauce brewing enzyme or protease (matching) enzyme etc. is one such or two kinds or more enzymes or bacterium add up to 0.3-1.3 jins;Or glutaminase and decarboxylase are added, add Dosage is between 100 jin 0.01--3 grams of soy meal, and within keeping temperature 48 hours, y- Gamma-propalanine content [dry meter] 100 Gram when in 100mg or more;
3, fresh bean dreg kills miscellaneous bacteria or does not kill miscellaneous bacteria together with addition or 0.5 times of its volume -- 5 times of water, keeps temperature At 60 degree of celsius or less, sufu fermentation enzyme or sufu fermentation enzyme is added and adds dregs of beans hydrolase [or dregs of beans hydrolase adds soy sauce to make Make enzyme, one or two or more kinds of 0.3--1 jins of compound (matching) enzyme based on protease, between adjustment pH value 3-8,5, or again plus Enter glutamate decarboxylase, by [based on dry] 100 jin: 0.03-3 grams ratio additions, within being kept for temperature 48 hours;
4,100 jin of Mai Fupi powder, addition or 0.5 times of its volume -- 5 times of water, kill miscellaneous bacteria or do not kill miscellaneous bacteria, pH value Between 5- 8,5,55 degrees celsius of temperature or so are kept, sufu fermentation enzyme or dregs of beans water is added when reducing temperature and spending to 25-35 Solve that enzyme, sufu fermentation enzyme, compound (matching) enzyme based on protease or bacterium etc. are one such or two kinds or more enzymes add up to weight It 0.05-1.2 jins, is added into and keeps temperature fermentation within 48 hours after mixing evenly;Then ground it can become pulpous state liquid Body, or be reprocessed into as raw material or other commodity;
5, Mai Fupi powder adds 0.5 times of above 100 jin of one of okara powder or two kinds, addition or its volume -- 5 times Water kills miscellaneous bacteria or does not kill miscellaneous bacteria, between pH value 3-8,5, keeps that sufu fermentation enzyme is added when 60 degrees celsius of temperature or less, or Sufu fermentation enzyme adds one such or two kinds or more enzymes or the bacterium such as compound (matching) enzyme based on dregs of beans hydrolase or protease 0.02-1.2 jins, be added into and after mixing evenly, keep temperature fermentation within 48 hours;Then ground it can become pulpous state liquid Body or drying or drying are reprocessed into as raw material or other commodity and product;
8, Mai Fupi powder, okara powder are one such or two at olecranon bean powder etc. plus mung bean flour or red bean or Peas powder Kind or more 100 jin, addition or 0.5 times of its volume -- 5 times of water, kill miscellaneous bacteria or do not kill miscellaneous bacteria, between pH value 3-9, keep Sufu fermentation enzyme or dregs of beans hydrolase, sufu fermentation enzyme, compound based on protease are added when below 60 degrees celsius of temperature (matching) enzyme or bacterium etc. be one such or two kinds or more enzymes or 0.2-1.2 jins of bacterium are added into and keep temperature fermentation after mixing evenly To within 48 hours;Then it ground can become paste liquid, or become raw material or other product commodity after reprocessing is dry;
9, Mai Fupi powder, okara powder add 100 jin of peanut meal powder, add or 0.5 times of its volume -- 5 times of water kills miscellaneous bacteria Or do not kill miscellaneous bacteria, between pH value 3-8,5, keep being added when 60 degrees celsius of temperature or less sufu fermentation enzyme or dregs of beans hydrolase, Compound (matching) enzyme, bacterium based on sufu fermentation enzyme or protease etc. be one such or two kinds or more enzymes or 0.2-1.2 jins of bacterium, adds Enter into and keeps temperature fermentation within 48 hours after mixing evenly;Then ground it can become paste liquid, or through reprocessing As raw material or other commodity;
10, Mai Fupi powder, okara powder: soybean or the beans [peanut] of semen sojae atricolor, brown or paint face, red bean, mung bean, bowl 100 jin of one such or two kinds or more extract powder such as the miscellaneous bean varieties grain reality of the big grain such as beans, chick-pea or particle etc., Addition or 0.5 times of its volume -- 6 times of water, kill miscellaneous bacteria or do not kill miscellaneous bacteria, between pH value 3-8,5, keep 60 celsius of temperature Addition sufu fermentation enzyme or dregs of beans hydrolase when degree is following, sufu fermentation enzyme, compound (matching) enzyme based on protease or bacterium etc. its One of or two kinds or more enzyme or 0.2-1.1 jins of bacterium keep temperature fermentation within 48 hours after mixing evenly;Then may be used It is ground to become paste liquid, or be reprocessed into as raw material or other commodity;
11, Mai Fupi powder, okara powder, peanut meal powder plus mung bean flour or peanut meal powder plus mung bean flour or red bean or Peas powder or peanut meal powder are one such or two kinds or more plus mung bean flour or red bean or Peas powder or olecranon bean powder etc. It 100 jin, kills miscellaneous bacteria or does not kill miscellaneous bacteria, between pH value 3-8,5, keep that sufu fermentation is added when 60 degrees celsius of temperature or less One such or two kinds or more the enzymes such as enzyme or dregs of beans hydrolase, sufu fermentation enzyme, compound (matching) enzyme based on protease or bacterium Or 0.5-1.3 jins of bacterium, it is added into and keeps temperature fermentation within 48 hours after mixing evenly;Then ground it can become beans Paste liquid, or food additives are reprocessed or added as commodity or raw material or other commodity;
It is used based on sufu fermentation enzyme or dregs of beans hydrolase or sufu fermentation enzyme, protease in the technical solution of the present invention Compound (matching) enzyme or bacterium one or two or more kinds when, in enzymatic hydrolysis within 48 hours, or add sufu fermentation simultaneously Enzyme or dregs of beans hydrolase, sufu fermentation enzyme are one such or two kinds or more, and content can generate biography when being greater than 0,1% or more ' fermentation ' phenomenon in system meaning: it such as turns sour, in the secondary substance of micro-perfume taste;Addition acid, alkali, salty, fragrant, sweet tea etc. can be passed through ' food additives ' adjust or optimize mouthfeel and food quality;Or added with the substance for being rich in glutamic acid-type ingredient, this method Use the substrate of the powderies such as Mai Fupi powder, the okara powder can be made to produce [dry meter] 300mg/100 grams or so y- aminobutyric acid The product of content.
Okara powder feature in the present invention refers to include soya bean belonging to soybean, semen sojae atricolor, green soya bean, sepia beans or flower Bean dregs powdery or granular substance after the bean varieties grain reality of face and its processing;Soybean cake dregs powder, peanut dregs powder, which refer to, includes The miscellaneous beans etc. of the big grain such as red bean, mung bean, Peas, chick-pea also includes processed extracted above-described big Beans, peanut, red bean, mung bean, Peas, the powder of the protein of chick-pea and other ingredients and its ground-slag substance of beans.
In the present invention, the content of y- aminobutyric acid is by the powder after enzymatic hydrolysis or fermentation by amino acid solution Extraction, pass through obtained by ion exchange and amino acid analysis method using to clear liquid thereon;Ion exchange can also be utilized It method and is obtained through amino-acid analyzer;Can also by the soy meal drying after, using the methods of spectroanalysis instrument into Row detects its content.
Adding using technical characteristic is mainly sufu fermentation enzyme or dregs of beans hydrolase or sufu fermentation enzyme or containing albumen One of various complex enzymes of enzyme or two kinds or more are the general designation by plant produced or containing zymogenic commodity complex enzyme; Or can also by the enzymes such as protease, cellulose fermentoid, zytase or zymophyte by market purchasing, voluntarily prepare after apply.
A kind of Mai Fupi powder, okara powder application generate mono- aminobutyric acid food of high-content y, be using sufu fermentation enzyme or The function of compound (matching) enzyme or bacterium and Glutamic acid decarboxylase based on dregs of beans hydrolase or protease, prepares high-content y The pasty food or Mai Fupi powder, okara powder of one aminobutyric acid, or by the mixed powder class of 2 kinds of powder, or with wheat flour or big Rice flour or coarse cereals are one such or two kinds or more of powder is mixed into commercial flours;Can also become again added acid, alkali, The liquid substance of the food additives such as salty, fragrant, sweet tea;Or reprocessing is formed as other food goods;It can also be in zymotechnique In the production and processing application for directly interpolating bread or steamed bun or other flour-made foods as raw material after the completion;Or through being dried Become such as stick, ball, ball, the piece product commodity of various Hun He Mian powder and its various different shapes after processing.
Due to can will be after fermentation rich in having the liquid of the y- aminobutyric acid directly as finished market product or by the wheat husband Powder, okara powder dietary fiber solids as raw material be added to production in use;Both its y- aminobutyric acid can be improved to receive Rate is increased economic efficiency, and sewage discharge can also be reduced, energy saving, favorable environment protection, meets current related green hair The policy of exhibition.
Market prospects
Nearly 200,000,000 people of China overweight crowd, various chronics are more than 300,000,000 people;Health is pursued, spirit of releiving pressure Power reduces the generation of chronic patient patient;There is 60 years old or more population more than 200,000,000 in China, wherein about 20% or more differently suffers from Constipation and hyperlipidemia hyperglycemia obesity etc. ' rich people's disease ';It is insoluble rich in pectic substance lignin soluble dietary fiber Crude fibre [soyabean oligosaccharides] is hardly decomposed by the digested enzyme of human body, and is the nutrient of beneficial flora and prebiotic in enteron aisle Source.And can water swelling in vivo, can be hydrated emulsification enteron aisle inner lipid;Especially its water absorption rate has absorption up to 85% or so The endotoxic characteristic of human body intestinal canal can then make intake person prevent heart disease diabetes colorectal cancer diverticulum disease gall stone and just The ciril diseases such as secret;And pH value in adjustable enteron aisle, inhibit the generation of pathogen development breeding and putrefaction;Dietary fiber can make Intake person will not generate heat and energy, therefore, it is possible to reduce human body obesity and it is overweight.Thus, this is a huge need Ask market.
Its daily staple food allows the Mai Fupi powder of the aminobutyric acid containing y-, the dietary fiber of okara powder by biotechnology applications And slurry,
And its have additional nutrients in food and body & mind health can be obtained, it is target of the invention!So increasing The powder containing Mai Fupi of new healthy functions, the slurry of okara powder and y- aminobutyric acid, powder and other food compositions increase again for consumer New selection in the market is added;And because it is with new healthy functions, biotechnology applications and environmental protection production, materials safety day So or in beans are fragrant or other fragrance, and it is cheap and good-quality the features such as.For the hair for realizing daily 25-30 grams of dietary fiber per capita It opens up target and reduces fat, Overweight people and the high rate for suffering from chronic diseases patient, therefore will receive consumer and like and welcome!
Economic and Efficiency Analysis
In terms of the prefecture-level region of 1,000,000 or more populations, 1,000,000 yuan of purchase of equipment are about invested, 10--20 people operates production, often It can such as produce 20 tons this contain soy meal y- aminobutyric acid soymilk, Huo Mian food or other dietary fiber foods;If with Commodity product(s) per ton is made a profit 2000 yuan and is counted, and annual make a profit will reach nearly ten million yuan.Along with can be to due to a lack of dietary fiber and y- The adjuvant treatment and its health benefit of the sub-health population of aminobutyric acid reduce the obesity and overweight history chronic disease of human body therefrom The generation of human patient increases healthy and happy;Its treatment cost, the expense of expenses for medicine and other expenditures and the increased labor saved Power and social productive forces, generated economic benefit will earn a profit more plus social benefit, its comprehensive benefit than enterprise It is very big!
So for consumer groups, safe facilitates economically to absorb mono- aminobutyric acid product of y in daily life, the present invention Practical application and great dissemination are had more with its product.

Claims (8)

1. a kind of Mai Fupi powder or okara powder generate the preparation method of the fiber diet of aminobutyric acid containing y-, it is technically characterized in that Wheat husband powder or okara powder or two kinds of mixed powder are predominantly added up to 100 jin, add the water within 6 times of its volume, adjusts pH Between value 3-10, addition is mainly sufu fermentation enzyme or dregs of beans hydrolase or soy sauce brewing enzyme or the complex enzyme containing protease One of or two kinds or more of enzyme, 0.002-1.5 jins of usage amount;Make its act on substrate generate amino acid, be converted into for Raw material of the ingredient of aminobutyric acid containing y- as food.
2. a kind of Mai Fupi powder or okara powder generate the preparation method of the fiber diet of aminobutyric acid containing y-, it is technically characterized in that Predominantly by wheat husband powder or okara powder or more than 2 kinds of powder add up to 100 jin, add the water within 6 times of its volume, or go out to raw material Kill miscellaneous bacteria;It when below 60 degrees celsius, adjusts between pH value 3-10, being added is mainly sufu fermentation enzyme or dregs of beans hydrolase or sauce Oil brewing enzyme or the complex enzyme containing protease etc. is one such or two kinds or more or can form the enzyme 0.002-1.5 of fermentation Jin;Or separately it is added with the material containing glutamic acid;Keep enzymatic hydrolysis or within yeastiness 50 hours;Or removal excessive moisture is through drying As commodity powder or other products;Or it is ground again or be crushed into liquid such as pulpous state class commodity etc.;Or by the liquid material containing powder Upright connect inserts in the production and processings of flour-made foods such as bread steamed bun;Or dehydration is made for the food of the powder of husband containing wheat or okara powder The commodity of product or various forms;Or processing or be the y- with a kind of in wheat flour or rice meal or coarse cereal powder or two kinds of ingredients The mixed flour of aminobutyric acid.
3. according to claim 1 the method: it is technically characterized in that predominantly wheat husband powder or 100 jin of the particle of wheat, addition 0.3 times of its volume -- 6 times of water, until adjusting pH value at about 60 degree or less between 3-10;Be added be mainly sufu fermentation enzyme or 0.002-1.5 jins of complex enzyme based on sufu fermentation enzyme bacterium and dregs of beans hydrolase or protease;Keep enzymatic hydrolysis or yeastiness;Again Become soya-bean milk shape liquid commodity, or processed other food goods as various forms again after grinding.
4. according to claim 1 the method: being technically characterized in that predominantly bean curd ground-slag or soybean ground-slag or soybean skin powder 100 Jin, addition or 0.3 times of its volume -- 6 times of water, are killed miscellaneous bacteria or do not kill miscellaneous bacteria, adjusted when within 60 degrees celsius of celsius PH value is compound based on addition predominantly sufu fermentation enzyme or dregs of beans hydrolase or soy sauce brewing enzyme or protease between 3-10 Enzyme etc. is one such or two kinds or more enzymes add up to 0.002-1.5 jins, and within keeping temperature 50 hours.
5. according to claim 1 the method: it is technically characterized in that predominantly wheat husband powder adds okara powder or increases skin of beancurd powder 100 Jin, addition or 0.3 times of its volume -- 6 times of water, kill miscellaneous bacteria or do not kill miscellaneous bacteria, when being kept for 60 degree of temperature or less, adjust PH Complex enzyme etc. between value 3-9,5 based on addition predominantly sufu fermentation enzyme or dregs of beans hydrolase or sufu fermentation enzyme or protease One of or 0.02-1.5 the jin of enzyme of two kinds or more, holding temperature was to 4 hours or more after mixing evenly;Then can it is ground at For soya-bean milk shape liquid commodity,;Or it is reprocessed into after artificial drying method as raw material or other commodity.
6. according to claim 1 the method: it is technically characterized in that predominantly wheat husband powder or adds 100 jin of fresh bean dreg, addition or 0.5 times of its volume -- 6 times of water kills miscellaneous bacteria or does not kill miscellaneous bacteria, adjustment pH value 3-9 when being kept for 60 degree of temperature or less, 5 it Between, it is one of or two kinds that complex enzyme based on sufu fermentation enzyme or dregs of beans hydrolase or sufu fermentation enzyme or protease etc. is added 0.02-1.5 jins of above enzyme keeps temperature to 10 hours or more after mixing evenly;Then ground it can become paste liquid;Or It is reprocessed into as raw material or other commodity;
7. according to claim 1 or claim 2 the method: it is technically characterized in that predominantly sufu fermentation enzyme or dregs of beans One of hydrolase or sufu fermentation enzyme or various complex enzymes containing protease or two kinds or more enzymes, are by plant produced system At sale or by market sale or containing zymogenic commodity complex enzyme general designation;Or it can also be by protease, cellulose family The enzymes such as enzyme, zytase or zymophyte by market purchasing, voluntarily prepare after apply.
8. being technically characterized in that can also be by beans seed through high temperature according to claim 1 or claim 2 the method Steam or extruding and puffing technology or the mode working process for being equivalent to the technology, instantaneous deodorant flavouring;Make big beans or cereal Seed produces apparent fragrance, produces the aminobutyric acid liquid material of y- containing high-content using crushing as the inventive method Raw material for standby.
CN201711245271.3A 2017-11-21 2017-11-21 A kind of Mai Fupi powder or okara powder generate the preparation method of the fiber diet of aminobutyric acid containing y- Pending CN109805259A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111280395A (en) * 2020-03-10 2020-06-16 东北农业大学 Preparation method of bean dreg enzyme

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US5472730A (en) * 1994-02-01 1995-12-05 Director General Of Chugoku National Agricultural Experiment Station, Ministry Of Agriculture, Forestry And Fisheries γ-aminobutyric acid-enriched food material and method for producing γ-aminobutyric acid
JP2002045138A (en) * 2000-05-25 2002-02-12 Hayashibara Biochem Lab Inc SOYBEAN PROCESSED FOOD RICHLY CONTAINING gamma-AMINOBUTYRIC ACID
CN101810322A (en) * 2009-02-20 2010-08-25 南京工业大学 Method for producing food rich in gamma-aminobutyric acid
CN103380881A (en) * 2012-06-20 2013-11-06 王文华 Soybean meal or wheat bran powder with high content of y-aminobutyric acid and preparation method of food

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5472730A (en) * 1994-02-01 1995-12-05 Director General Of Chugoku National Agricultural Experiment Station, Ministry Of Agriculture, Forestry And Fisheries γ-aminobutyric acid-enriched food material and method for producing γ-aminobutyric acid
JP2002045138A (en) * 2000-05-25 2002-02-12 Hayashibara Biochem Lab Inc SOYBEAN PROCESSED FOOD RICHLY CONTAINING gamma-AMINOBUTYRIC ACID
CN101810322A (en) * 2009-02-20 2010-08-25 南京工业大学 Method for producing food rich in gamma-aminobutyric acid
CN103380881A (en) * 2012-06-20 2013-11-06 王文华 Soybean meal or wheat bran powder with high content of y-aminobutyric acid and preparation method of food

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111280395A (en) * 2020-03-10 2020-06-16 东北农业大学 Preparation method of bean dreg enzyme

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