CN109793801A - It is a kind of improve immunity substance and its application - Google Patents
It is a kind of improve immunity substance and its application Download PDFInfo
- Publication number
- CN109793801A CN109793801A CN201711140044.4A CN201711140044A CN109793801A CN 109793801 A CN109793801 A CN 109793801A CN 201711140044 A CN201711140044 A CN 201711140044A CN 109793801 A CN109793801 A CN 109793801A
- Authority
- CN
- China
- Prior art keywords
- rubber tree
- seeds
- solvent
- fatty contents
- substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention discloses a kind of seeds of rubber tree fatty contents and its application.Substance of the invention is the fatty contents of the seed of rubber tree, can be applied to drug, health food, in food, for improving the immunity of the human body.The substance is made with the kernel for drying the seeds of rubber tree after decladding by the methods of mechanical presses method, extraction method, defibrination water substitution, supercritical extraction.User passes through daily to take or the edible drug, health food, food for being added to the substance can be obtained the good effect for improving immunity.
Description
Technical field
The invention belongs to biomedicine fields, and specifically the present invention relates to a kind of substance (rubbers improved the immunity of the human body
Gum seed fatty contents) and its application.
Background technique
Immunity be human bioequivalence and eliminate exotic invasive any foreign matter, processing aging, damage, death, denaturation from
Body cell and identification and the ability for handling vivo mutations cell and virus infected cell are human bioequivalences and exclude " dissident "
Physiological reaction is the defense mechanism of human body itself.
The case where there have been hypoimmunities from normally playing protective effect because a variety of causes prevents immune system:
1, hypoimmunity is usually expressed as loss of appetite, malnutrition, apathetic, fatigue and weak, sleep disturbance etc.；
2, to children, lead to physical and intellectual depauperation；
3, body is easy to be infected by bacterium, virus, fungi etc., frequent illness；
4, after illness, recovery is takeed a long time, and be easy recurrence；
5, it is easy to induce major disease, the incidence of various cancers obviously increases.
There are many methods for improving human immunity, including drug therapy, and intake is containing the active material for being improved immunity
Health food or food, physical training, rule work and rest strike a proper balance between work and rest, avoid over fatigue etc..
Body immunity is improved, it is not only significant to the personage of hypoimmunity, also keep fit very to general population
There is benifit.
Summary of the invention
The purpose of the present invention is to provide a kind of substance (rubber tree species that some does not improve the immunity of the human body in the prior art
Sub- fatty contents) and its preparing drug, health food, the application in food.The substance is the liposoluble of the seed of rubber tree
Property part can be applied to drug, health food, in food, for improving the immunity of the human body.User pass through daily take or
The edible drug, health food, food for being added to the substance of person can be obtained the good effect for promoting immunity.
In order to realize above-mentioned purpose of the invention, the present invention provides the following technical solutions:
Seeds of rubber tree fatty contents, the seeds of rubber tree fatty contents are prepared by any one of following methods:
Mechanical presses method: using virgin rubber tree species as raw material, after drying shelling, kernel is obtained, kernel material is before extrusion
It is heated to 80-100 DEG C, then by static squeeze or dynamic screw extrusion, extrusion liquid is rubber tree
Seed fatty contents；
Extraction method: using virgin rubber tree species as raw material, after drying shelling, obtaining kernel, and kernel is carried out extruding, is granulated
Or it is rolling embryo with leaching container that is after processing, being put into sealing, solvent is added, selected solvent is soybean extracting solvent no.6, boiling
One of 30-90 DEG C of point range of petroleum ether, n-hexane, ether, ethyl acetate, n-butanol, or wherein several mixing
Object is stirred and mixes with solvent to material under the temperature condition and normal pressure lower than solvent boiling point, or carries out ultrasonic wave
Processing, allows material to come into full contact with solvent, staticly settles later, leaches solvent, then in the temperature for being higher than solvent boiling point and very
Under empty condition, solvent is allowed to volatilize, the liquid stayed is seeds of rubber tree fatty contents；
Supercritical extract: using virgin rubber tree species as raw material, after drying shelling, obtaining kernel, and kernel is carried out extruding, is granulated
Or after rolling embryo processing, it is put into the supercritical extract container of sealing pressure resistance, solvents carbon dioxide is added, is being more than solvent critical
Under conditions of temperature and critical pressure, material is stirred and is mixed with solvent, or carry out ultrasonication, allow material and
Solvent comes into full contact with.It staticly settles later, leaches solvent, then at room temperature, reduce pressure, allow solvent to volatilize, leave
The liquid come is seeds of rubber tree fatty contents.
Invention also provides the seeds of rubber tree fatty contents containing therapeutically effective amount and pharmaceutically may be used
The pharmaceutical composition of the carrier of receiving.
And health food, it is made of the seeds of rubber tree fatty contents and health food common carrier.
With food is made of the seeds of rubber tree fatty contents and food common carrier.
The present invention also provides the seeds of rubber tree fatty contents in the drug that preparation improves the immunity of the human body
Using.
Application as mentioned, wherein the seeds of rubber tree fatty contents are as sole active agent.
Present invention further provides the guarantors that the seeds of rubber tree fatty contents are improved the immunity of the human body in preparation
Application in health food.
And application of the seeds of rubber tree fatty contents in the food that preparation improves the immunity of the human body.
Application as mentioned, wherein the seeds of rubber tree fatty contents are as sole active agent.
According to the experimental data of animal model, and other experimental datas, treated with existing for user
Compared with the drug of rehabilitation, the present invention has the advantage that
A kind of substance that some does not improve the immunity of the human body in the prior art is provided.
Safety is good.Both it can use, can also be used for the crowd of hypoimmunity for general population, it can also be for pregnant
Patient after woman, juvenile, the elderly, operation and chemicotherapy uses.
It is without side-effects, it can be used for a long time for all kinds of users.
Specific embodiment
Below by the embodiment of the present invention, property content is described further for the essence of the present invention, but is not limited with this
The fixed present invention.
Embodiment 1
Mechanical presses method prepares seeds of rubber tree fatty contents, and institute's choosing method is static extrusion.With 100kg virgin rubber tree
Seed is raw material, after drying shelling, prepares kernel 40kg.Kernel material arrives 80-100 DEG C in heat, then squeezes by static
Pressure, pressure 50MPa, squeezing out 12kg liquid is substance seeds of rubber tree fatty contents of the invention.
120 BALB/c female mices of experimental selection, weight range 18-22g, are allocated as 8 groups, are respectively: i by every group 15
Blank control group gives chow diet；Ii. substance low dose group of the present invention is given and is added to low dosage substance rubber of the present invention
(additive amount of the substance in feed is 12.5g/kg to the feed of the sub- fatty contents of tree species, and mouse daily feed intake is its body
The 1/5 of weight, mouse are 2.5g/kg to the daily intaking amount of the substance, are equivalent to 30 times of human body low dosage 83mg/kg)；Iii. originally
Invention substance high dose group is given and is added to the feed of high dose substance of the present invention (additive amount of the substance in feed be
75g/kg, mouse daily feed intake are the 1/5 of its weight, and mouse is 15g/kg to the daily intaking amount of the substance, are equivalent to human body
30 times of high dose 500mg/kg)；Iv. high dose A substance control group gives the feed for being added to high dose A substance, A substance
For flex seed fatty contents, the additive amount in feed is 75g/kg, and mouse daily feed intake is the 1/5 of its weight, small
Mouse is 15g/kg to the daily intaking amount of the substance, is equivalent to 30 times of human body high dose 500mg/kg；V. high dose B substance compares
Group gives the feed for being added to high dose B substance, and B substance is purple perilla seed fatty contents (Purple Perilla Seed Oil), in feed
Additive amount is 75g/kg, and mouse daily feed intake is the 1/5 of its weight, and mouse is 15g/kg, phase to the daily intaking amount of the substance
When in 30 times of human body high dose 500mg/kg；Vi. high dose C substance control group gives the feeding for being added to high dose C substance
Material, C substance are maize germ fatty contents (maize germ oil), and the additive amount in feed is 75g/kg, and mouse is adopted daily
Appetite is the 1/5 of its weight, and mouse is 15g/kg to the daily intaking amount of the substance, is equivalent to the 30 of human body high dose 500mg/kg
Times；Vii. high dose D substance control group, gives the feed for being added to high dose D substance, and D substance is olive fruit fatty contents
(olive oil), the additive amount in feed are 75g/kg, and mouse daily feed intake is the 1/5 of its weight, and mouse is to the substance
Daily intaking amount is 15g/kg, is equivalent to 30 times of human body high dose 500mg/kg；Viii. high dose E substance control group, gives and adds
The feed of high dose E substance is added, E substance is Pumpkin Seed fatty contents (pumpkin seed oil), and the additive amount in feed is
75g/kg, mouse daily feed intake are the 1/5 of its weight, and mouse is 15g/kg to the daily intaking amount of the substance, are equivalent to human body
30 times of high dose 500mg/kg.
It is fed 30 days by above grouping and rearing conditions.
This example detects the influence of contrivance confrontation animal pattern immunity with delayed allergy (vola pedis thickens method).
De- fiber sheep blood is taken, brine 3 times, each 2000rpm, is centrifuged 10 minutes, discards supernatant, use physiology salt
Water is configured to 2%(v/v) SRBC, one group of every mouse peritoneal is immunized and injects 0.2ml, after sensitization 4 days, with a vernier caliper measurement left side
Vola pedis thickness afterwards, same position measurement is three times.Same method is configured to 20%(v/v with physiological saline) SRBC, in measuring point skin
Lower injection 0.02ml20%(v/v) SRBC, left back vola pedis thickness is measured within 24 hours after injection, same position measurement is three times.With attack
Front and back pedal swelling degree indicates delayed allergy degree.
Experimental result is as follows:
| Group | Number of animals (only) | Swelling degree of the paw (mm) | P value |
| Blank control group | 15 | 0.52±0.29 | -- |
| Substance low dose group of the present invention | 15 | 0.80±0.25 | P<0.05 |
| Substance high dose group of the present invention | 15 | 0.94±0.43 | P<0.01 |
| High dose A substance control group | 15 | 0.55±0.29 | P>0.05 |
| High dose B substance control group | 15 | 0.54±0.29 | P>0.05 |
| High dose C substance control group | 15 | 0.50±0.29 | P>0.05 |
| High dose D substance control group | 15 | 0.51±0.29 | P>0.05 |
| High dose E substance control group | 15 | 0.52±0.29 | P>0.05 |
The experimental results showed that mouse has eaten the feed for being added to substance of the present invention, either high dose group or low dosage
Group, opposite blank control group can enhance its immunity (intensity for increasing delayed allergy), statistically have significant
(low dose group P < 0.05) and extremely significant difference (high dose group P < 0.01).
Experimental result is also shown that the feed of the edible fatty contents for being added to other several oil plants of mouse, mouse immune
Power (intensity of delayed allergy) and blank control group are not significantly different (P > 0.05).
Embodiment 2
Mechanical presses method prepares seeds of rubber tree fatty contents, and institute's choosing method is screw extrusion method.With 100kg virgin rubber tree
Seed is raw material, after drying shelling, prepares kernel 40kg.Kernel material arrives 80-100 DEG C in heat, then passes through dynamic spiral shell
Rotation squeezes, and the screw extrusion time is 45 seconds or so, and squeezing out 15kg liquid is substance seeds of rubber tree liposoluble of the invention
Property part.
160 BALB/c female mices, feeding 30 are fed by method described in embodiment 1 and group with the substance of this example
It.
Mouse spleen lymphocyte transformation experiment (mtt assay) that this example is induced with ConA detects contrivance confrontation animal pattern
The influence of immunity.
Mouse is put to death, it is sterile to take spleen, it is placed in plate, is ground after being wrapped up with gauze with elbow tweezers, it is thin that spleen is made
Born of the same parents' suspension is washed in test tube with 7mlHank ' s liquid, and 1000rpm, 10min centrifugation are washed 2 times.It abandons supernatant to bounce cytoplasm, add
0.5mL aqua sterilisa 20 seconds, 0.5mL2 times of Hank ' s liquid and 8mLHank ' s liquid, 1000rpm, 10min are added after splitting erythrocyte
Centrifugation, the RPMI1640 complete culture solution with 1mL containing 10% calf serum are resuspended.20 μ L cell suspensions are taken to be added to the ice of 1mL1%
It in acetic acid, counts, adjustment cell concentration is 5 × 106/mL.It is divided to two holes to be added in 24 well culture plates cell suspension, every hole
1mL, 75 μ L ConA solution (being equivalent to 7.5 μ g/mL) are added in a hole wherein, and another hole is added equivalent sterile water and is used as control,
Carbon dioxide incubator 5%CO2 is set, 37 DEG C are cultivated 72 hours.Culture terminates first 4 hours, and every hole gently sucks supernatant 0.7mL,
The RPMI1640 culture solution without calf serum of isodose is added, while MTT(5mg/mL is added) 50 holes μ L/, continue culture 4
Hour.After culture, 1mL acid isopropyl alcohol is added in every hole, is completely dissolved purple crystal.Then with microplate reader in wavelength
Colorimetric estimation at 570nm.The proliferative capacity of lymphocyte with plus the absorbance value in the hole ConA subtract the absorbance in the hole ConA be not added
Value indicates.
Experimental result is as follows:
| Group | Number of animals (only) | Absorbance difference | P value |
| Blank control group | 15 | 0.18±0.07 | -- |
| Substance low dose group of the present invention | 15 | 0.27±0.05 | P<0.01 |
| Substance high dose group of the present invention | 15 | 0.26±0.05 | P<0.01 |
| High dose A substance control group | 15 | 0.18±0.06 | P>0.05 |
| High dose B substance control group | 15 | 0.19±0.07 | P>0.05 |
| High dose C substance control group | 15 | 0.17±0.08 | P>0.05 |
| High dose D substance control group | 15 | 0.18±0.06 | P>0.05 |
| High dose E substance control group | 15 | 0.19±0.05 | P>0.05 |
The experimental results showed that the edible feed for being added to substance of the present invention of mouse, either high dose group or low dose group,
Opposite blank control group can enhance its immunity (enhancing lymphopoiesis ability, i.e. the mice spleen leaching of increase ConA induction
Bar cell transformation rate), statistically there is extremely significant difference (P < 0.01).
Experimental result is also shown that the feed of the edible fatty contents for being added to other several oil plants of mouse, mouse immune
Power immunity (the mouse spleen lymphocyte conversion ratio of lymphopoiesis ability, i.e. ConA induction) is not shown with blank control group
It writes difference (P > 0.05).
Embodiment 3
Solvent extraction prepares seeds of rubber tree fatty contents, and extracting selected solvent is No. 6 solvents.With the fresh rubber of 100kg
Gum seed is that raw material prepares kernel 40kg after drying shelling.Then after kernel being carried out expanding treatment, it is put into sealing
Leaching container, the solvent of 2 times of weight is added.Under 55 DEG C of (be lower than solvent boiling point) conditions and normal pressure, to material and solvent into
Row is stirred and is mixed, and material is allowed to come into full contact with solvent.It staticly settles later, leaches solvent, then (be higher than solvent at 120 DEG C
Boiling temperature) under 100Pa vacuum condition, allow solvent to volatilize, staying 18kg liquid is substance rubber of the invention
The sub- fatty contents of tree species.
160 BALB/c female mices, feeding 30 are fed by method described in embodiment 1 and group with the substance of this example
It.
This example detects contrivance confrontation animal pattern immunity with antibody-producting cell detection (Jerne improve plectrum method)
Influence.
De- fiber sheep blood is taken, brine 3 times, is configured to 2%(v/v with physiological saline) SRBC, every mouse peritoneal
0.2ml is injected, after sensitization 5 days, cervical dislocation puts to death mouse, takes spleen, splenocyte suspension is made, full splenocyte is suspended in
In 8mLHank's liquid.1% agarose heating for dissolving is placed in 45 DEG C of water-baths to be mixed with 2 times of Hank's liquid of equivalent, dispenses lab scale
Pipe, every pipe 0.5mL, then the 10%(v/v for adding 50 μ LSA liquid to prepare into pipe) SRBC, the 20 full splenocyte suspensions of μ L, mixing rapidly,
It is poured on the slide brushed with 0.5% agarose thin layer, does parallel plate, slide level, which is buckled, puts to glass frame, carbon dioxide training
5%CO2 in case, 37 DEG C of incubation 1.5h are supported, the complement that SA liquid dilutes 10 times is added in glass frame groove, continues to be incubated for 1.5h, meter
Number hemolysis plaque number.Full spleen plaque number is that the splenocyte of 400(smear accounts for the ratio of full splenocyte) plaque number is counted multiplied by slide.
Experimental result is as follows:
| Group | Number of animals (only) | Hemolysis plaque number (× 102Full spleen) | P value |
| Blank control group | 15 | 402±198 | -- |
| Substance low dose group of the present invention | 15 | 629±290 | P<0.01 |
| Substance high dose group of the present invention | 15 | 775±221 | P<0.01 |
| High dose A substance control group | 15 | 469±190 | P>0.05 |
| High dose B substance control group | 15 | 490±229 | P>0.05 |
| High dose C substance control group | 15 | 510±167 | P>0.05 |
| High dose D substance control group | 15 | 535±257 | P>0.05 |
| High dose E substance control group | 15 | 444±206 | P>0.05 |
The experimental results showed that the edible feed for being added to substance of the present invention of mouse, either high dose group or low dose group,
Opposite blank control group can enhance its immunity (increasing the quantity of antibody-producting cell, i.e. increase hemolysis plaque number), unite
Meter has extremely significant difference (P < 0.01) on learning.
Experimental result is also shown that the feed of the edible fatty contents for being added to other several oil plants of mouse, mouse immune
Power (quantity of antibody-producting cell, i.e. hemolysis plaque number) and blank control group are not significantly different (P > 0.05).
Embodiment 4
Supercritical extraction prepares seeds of rubber tree fatty contents, and selected solvent is carbon dioxide.With 100kg virgin rubber
Tree species is that raw material prepares kernel 40kg after drying shelling.After kernel is carried out granulation processing, it is put into sealing pressure resistance
Supercritical extract container, is added solvent, and solvent quality is 2 times of quality of material.At 35 DEG C, 10MPa(is more than the stagnation temperature of solvent
Degree and critical pressure) under conditions of, material is stirred and is mixed with solvent, material is allowed to come into full contact with solvent.It is quiet later
Precipitating is set, solvent is leached, then under room temperature at 25 DEG C, reduces pressure to normal pressure (0.1MPa), allows solvent to volatilize, stay
18kg liquid is substance seeds of rubber tree fatty contents of the invention.
160 BALB/c female mices, feeding 30 are fed by method described in embodiment 1 and group with the substance of this example
It.
This example is dynamic to detect contrivance confrontation model with Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell experiment (drop piece method)
The influence of object immunity.
De- fiber sheep blood is taken, brine 3 times, is configured to 2%(v/v with physiological saline) SRBC, every mouse peritoneal
0.2ml is injected, after sensitization 4 days, cervical dislocation puts to death mouse.Hank ' the s liquid 4mL of intraperitoneal injection plus calf serum gently rubs abdomen,
Stomach wall chamber cuts off osculum, washes out 2mL in test tube.It takes the addition of 0.5mL washing lotion to be equipped in the test tube of 0.5mL1% chicken red blood cell, mixes
It is even.Syringe draws 0.5mL mixed liquor, is added in agar circle.It is incubated for 15min, is washed off with physiological saline rapidly, methanol solution is fixed
1min, Giemsa liquid 15min, distilled water flushing.It dries, 40 × microscopic counting.
Experimental result is as follows:
| Group | Number of animals (only) | Phagocytic percentage (%) | P value |
| Blank control group | 15 | 24.4±3.9 | -- |
| Substance low dose group of the present invention | 15 | 31.9±3.2 | P<0.01 |
| Substance high dose group of the present invention | 15 | 29.7±5.1 | P<0.01 |
| High dose A substance control group | 15 | 26.6±4.1 | P>0.05 |
| High dose B substance control group | 15 | 23.3±3.6 | P>0.05 |
| High dose C substance control group | 15 | 25.4±5.3 | P>0.05 |
| High dose D substance control group | 15 | 24.9±5.8 | P>0.05 |
| High dose E substance control group | 15 | 26.73±4.9 | P>0.05 |
The experimental results showed that the edible feed for being added to substance of the present invention of mouse, either high dose group or low dose group,
Opposite blank control group can enhance its immunity (phagocytic activity of enhancing macrophage, i.e. increase Turnover of Mouse Peritoneal Macrophages
Swallow the ability of chicken red blood cell), statistically there is extremely significant difference (P < 0.01).
Experimental result is also shown that the feed of the edible fatty contents for being added to other several oil plants of mouse, mouse immune
Power (ability of the phagocytic activity of macrophage, i.e. Turnover of Mouse Peritoneal Macrophages phagocytosis chicken red blood cell) is not shown with blank control group
It writes difference (P > 0.05).
Embodiment 5:
Seeds of rubber tree fatty contents of the invention are made by the method in embodiment 2-5, by the part and excipient weight
Excipient, pelletizing press sheet is added in ratio than 1:1 or 1:2.Tablet can be used as drug, health food or food.
Embodiment 6:
Seeds of rubber tree fatty contents of the invention are made by the method in embodiment 2-5 and are wrapped in soft capsule by the part
In capsule material, routinely soft capsule is made in capsule preparations method.Soft capsule can be used as drug, health food or food.
Embodiment 7:
Seeds of rubber tree fatty contents of the invention are made by the method in embodiment 2-5, with the part, in addition emulsifier,
Osmotic pressure regulator and water obtain emulsion for injection by high-pressure homogeneous emulsification.
Embodiment 8:
Seeds of rubber tree fatty contents of the invention are made by the method in embodiment 2-5, by the part, then as follows
Tablet is made:
Tablet: seeds of rubber tree fatty contents 100mg
Appropriate starch
Appropriate corn pulp
Magnesium Stearate proper quantity
Preparation method: seeds of rubber tree fatty contents are mixed with auxiliary agent, and sieving uniformly mixes in suitable container,
Obtained granulating mixture tabletting.
Embodiment 9:
Seeds of rubber tree fatty contents of the invention are made by the method in embodiment 2-5, by the part, then as follows
Soft capsule is made:
Soft capsule: seeds of rubber tree fatty contents 1000mg
Appropriate gelatin
Appropriate glycerol
Appropriate amount of water
Preparation method: gelatin, glycerol, which are put into distilled water, to impregnate makes gelatin expansion, softening, is then uniformly mixed, obtains
Capsule material glue.Prepared capsule material glue is taken out, is coated in flat plate surface, keeps thickness uniform, then with 90 DEG C or so
Temperature heating, evaporates surface moisture, becomes the cushion compound for having certain toughness, having certain elasticity.Finally with pelleting mold or
Automatic rotation rolling capsule machine, seeds of rubber tree fatty contents (1000mg) are fitted into soft capsule skin, soft capsule is produced.
Embodiment 10:
Seeds of rubber tree fatty contents of the invention are made by the method in embodiment 2-5, by the part, then as follows
Emulsion for injection is made:
Emulsion for injection: seeds of rubber tree fatty contents 200g
Appropriate lecithin
Appropriate glycerol
Appropriate sodium chloride
Appropriate amount of water
Preparation method: according to formula, by seeds of rubber tree fatty contents, lecithin, glycerol mixing adds water to 1000mL, adds
Appropriate sodium chloride adjusts osmotic pressure, high-pressure homogeneous emulsification, and 121 DEG C of sterilizings are down to room temperature to get emulsion for injection is arrived.
Embodiment 11:
Tablet: being made seeds of rubber tree fatty contents 10mg, lactose 180mg of the invention by the method in embodiment 2-5, forms sediment
Powder 55mg, magnesium stearate 5mg；
Preparation method: seeds of rubber tree fatty contents of the invention, newborn sugar and starch will be made by the method in embodiment 2-5
Mixing, is uniformly moistened with water, and the mixture after moistening is sieved and is dried, re-sieving, magnesium stearate is added, then by mixture
Tabletting, every slice weight 250mg, seeds of rubber tree fatty contents content of the invention are 10mg.
Embodiment 12:
Capsule: seeds of rubber tree fatty contents 2000g of the invention, gelatin is made by the method in embodiment 2-5
1000g, glycerol 500g, water 1000g.
Preparation method: by gelatin 1000g, glycerol 500g, being put into 1000g water to impregnate makes gelatin expansion, softening, then stirs
It mixes uniformly mixed, obtains capsule material glue.Prepared capsule material glue is taken out, is coated in flat plate surface, keeps thickness uniform, so
It is heated afterwards with 90 DEG C or so of temperature, evaporates surface moisture, become the cushion compound for having certain toughness, having certain elasticity.Finally
With pelleting mold or automatically rolling capsule machine is rotated, seeds of rubber tree fatty contents 1000mg is fitted into soft capsule skin, be made
Soft capsule.Every soft capsule weight 1.4g, seeds of rubber tree fatty contents content of the invention is 1000mg.
Embodiment 13:
Emulsion for injection: being made seeds of rubber tree fatty contents 200g, glycerol 25g of the invention by the method in embodiment 2-5,
Lecithin 12g, water 1000g, sodium chloride 1g.
Preparation method: accurate weighing by the method in embodiment 2-5 be made seeds of rubber tree fatty contents 200g of the invention,
Glycerol 25g, lecithin 12g, mixing add water to 1000mL, add sodium chloride, osmotic pressure is adjusted to 350mosm/kg H2O。
High-pressure homogeneous emulsification obtains emulsion, and 121 DEG C of sterilizings are down to room temperature, are aseptically filled to the sterile glass vials of 250mL
Middle encapsulation.Every bottle of emulsion for injection specification is 250mL, and seeds of rubber tree fatty contents content of the invention is 50g.
Embodiment 14:
Food: being made seeds of rubber tree fatty contents of the invention by the method in embodiment 2-5, by the part and common food
It is reconciled with oil, is directly becoming food (edible oil).
Preparation method: seeds of rubber tree fatty contents of the invention are made by the method in embodiment 2-5 in 10kg, addition
Into 10kg palm oil, 20kg blending edible oil is obtained.By the filling vial at 500g of blending edible oil, rubber of the invention
The sub- fatty contents content of tree species is 250g.
Claims (10)
1. seeds of rubber tree fatty contents, it is characterised in that the seeds of rubber tree fatty contents are by any in following methods
Kind is prepared:
Mechanical presses method: using virgin rubber tree species as raw material, after drying shelling, kernel is obtained, kernel material is before extrusion
It is heated to 80-100 DEG C, then by static squeeze or dynamic screw extrusion, extrusion liquid is rubber tree
Seed fatty contents；
Extraction method: using virgin rubber tree species as raw material, after drying shelling, obtaining kernel, and kernel is carried out extruding, is granulated
Or it is rolling embryo with leaching container that is after processing, being put into sealing, solvent is added, selected solvent is soybean extracting solvent no.6, boiling
One of 30-90 DEG C of point range of petroleum ether, n-hexane, ether, ethyl acetate, n-butanol, or wherein several mixing
Object is stirred and mixes with solvent to material under the temperature condition and normal pressure lower than solvent boiling point, or carries out ultrasonic wave
Processing, allows material to come into full contact with solvent, staticly settles later, leaches solvent, then in the temperature for being higher than solvent boiling point and very
Under empty condition, solvent is allowed to volatilize, the liquid stayed is seeds of rubber tree fatty contents；
Supercritical extract: using virgin rubber tree species as raw material, after drying shelling, obtaining kernel, and kernel is carried out extruding, is granulated
Or after rolling embryo processing, it is put into the supercritical extract container of sealing pressure resistance, solvents carbon dioxide is added, is being more than solvent critical
Under conditions of temperature and critical pressure, material is stirred and is mixed with solvent, or carry out ultrasonication, allow material and
Solvent comes into full contact with, and staticly settles later, leaches solvent, then at room temperature, reduces pressure, allows solvent to volatilize, leave
The liquid come is seeds of rubber tree fatty contents.
2. seeds of rubber tree fatty contents described in claim 1 and pharmaceutically acceptable carrier containing therapeutically effective amount
Pharmaceutical composition.
3. health food is made of seeds of rubber tree fatty contents described in claim 1 and health food common carrier.
4. food is made of seeds of rubber tree fatty contents described in claim 1 and food common carrier.
5. application of the seeds of rubber tree fatty contents described in claim 1 in the drug that preparation improves the immunity of the human body.
6. application as claimed in claim 5, which is characterized in that the seeds of rubber tree fatty contents are as in drug
Sole active agent.
7. seeds of rubber tree fatty contents described in claim 1 answering in the health food that preparation improves the immunity of the human body
With.
8. application as claimed in claim 7, which is characterized in that the seeds of rubber tree fatty contents are as unique living
Property ingredient.
9. application of the seeds of rubber tree fatty contents described in claim 1 in the food that preparation improves the immunity of the human body.
10. application as claimed in claim 9, which is characterized in that the seeds of rubber tree fatty contents are as unique
Active constituent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711140044.4A CN109793801A (en) | 2017-11-16 | 2017-11-16 | It is a kind of improve immunity substance and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711140044.4A CN109793801A (en) | 2017-11-16 | 2017-11-16 | It is a kind of improve immunity substance and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109793801A true CN109793801A (en) | 2019-05-24 |
Family
ID=66555690
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711140044.4A Pending CN109793801A (en) | 2017-11-16 | 2017-11-16 | It is a kind of improve immunity substance and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109793801A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101475872A (en) * | 2009-01-12 | 2009-07-08 | 云南三环生物技术有限公司 | Method for extracting oil from rubber seed |
| CN106109274A (en) * | 2016-07-14 | 2016-11-16 | 西双版纳华坤生物科技有限责任公司 | Application of seeds of rubber tree oil and preparation method thereof |
-
2017
- 2017-11-16 CN CN201711140044.4A patent/CN109793801A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101475872A (en) * | 2009-01-12 | 2009-07-08 | 云南三环生物技术有限公司 | Method for extracting oil from rubber seed |
| CN106109274A (en) * | 2016-07-14 | 2016-11-16 | 西双版纳华坤生物科技有限责任公司 | Application of seeds of rubber tree oil and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 王慧敏: "橡胶籽油的提取、精炼及其氧化稳定性研究", 《中国优秀硕士学论文全文数据库工程科技Ι辑》 * |
| 陈建白: "橡胶种子油与ω-3脂肪酸", 《云南热作科技》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104306617B (en) | Composition containing maca and A Sayi fruits and its preparation method and application | |
| CN110013533B (en) | A Chinese medicinal composition with stomach nourishing, appetite stimulating, and digestion promoting effects for children, and its preparation method | |
| US10813961B2 (en) | Powder formulation having a function of enhancing immunity and method for preparing the same | |
| CN110604303A (en) | Rhizoma polygonati paste and preparation method thereof | |
| KR101139372B1 (en) | Method for preparing feed additive and feed additivethere from | |
| CN105124549B (en) | A kind of alimentation composition and preparation method thereof reducing radiation hazradial bundle | |
| CN102349608A (en) | Healthcare product combination for clearing and nourishing throat and preparation method thereof | |
| CN105125606B (en) | Improve the chewable tablets and preparation method thereof of immunity | |
| CN109793801A (en) | It is a kind of improve immunity substance and its application | |
| CN102836334B (en) | Veterinary Chinese medicinal preparation for replenishing qi to invigorate spleen | |
| CN109793771A (en) | It is a kind of improve immunity substance and its application | |
| CN109793805A (en) | It is a kind of improve immunity substance and its application | |
| CN102038857A (en) | Method for preparing Chinese medicinal composition for treating child dyspepsia caused by overeating | |
| CN104352940B (en) | A kind of Chinese medicine composition for alleviating physical fatigue | |
| CN106668077A (en) | Marine bioactive composition and pharmaceutical preparation | |
| CN108403852A (en) | A kind of cordyceps sinensis Grifola frondosa functional food and its preparation method and application | |
| CN109793780A (en) | It is a kind of for the hypoglycemic substance of type II diabetes, preparation method and applications | |
| CN105770187A (en) | Health food capable of improving immunity and preparing method thereof | |
| CN109793807A (en) | A kind of substance and preparation method and purposes for preventing and treating parkinsonism | |
| KR20140118329A (en) | Method for manufacturing pill using concentrated herb medicine solution and herb powder | |
| CN104042790B (en) | A kind of pharmaceutical composition and its application for health care for the middle and old aged | |
| CN103494203A (en) | Healthcare food with immunity boosting function | |
| CN108851071A (en) | Sea-buckthorn VP alimentation composition and its preparation method and application | |
| CN109793775A (en) | It is a kind of for preventing and treating the substance and purposes of hyperlipidemia | |
| CN107233519A (en) | A kind of composition and its preparation method and purposes for treating constipation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190524 |
|
| RJ01 | Rejection of invention patent application after publication |