The present invention relates to the health food technology field, particularly a kind of health food with enhancing immunity function.
Immune system is body antagonism exotic disease pathogenic microorganism; defence system as the invasion and attack of the infections sources such as bacterium, virus. the complex networks system that it is comprised of leucocyte, lymphocyte, macrophage, NK cell, Dc cell and antibody forming system etc.; when body is impaired; it can protect body to avoid the pathogenic microorganism invasion and attack such as alien bacteria, virus; the cancer cell formed due to gene mutation in the time of also can identifying with the scavenger Somatic Embryogenesis is heard, and in maintaining body health, prophylactic generation and development, has very important significance.
The health that shows as of hypoimmunity is easy to infected or cancer stricken; Immunity is extraordinary also can produce insalubrious result, as caused allergic reaction, autoimmune disease etc.Because of often ill, increased the weight of the consumption of body, thus generally have a delicate constitution, the performances such as malnutrition, listlessness, fatigue and weak, appetite reduction, sleep-disorder, sick, have an injection and take medicine the normal potluck that just gets married.Each sickly all will could recover for a long time, and usually outbreak repeatedly.If things go on like this can cause health and intelligence development bad, also easily bring out major disease.
Underlying causes is that hypoimmunity or immunity are unsound.When the immune function of human body imbalance, when perhaps immune system is unsound, following point will show effect repeatedly:--flu outbreak repeatedly, tonsillitis shows effect repeatedly, asthma is shown effect repeatedly, bronchitis is shown effect repeatedly, pneumonia is shown effect repeatedly, suffer from diarrhoea and repeatedly show effect etc. you, so necessarily can't neglect.
For thousands of years, traditional Chinese medicine is being prevented and cured diseases, the aspect such as improve the health plays an important role, a large amount of clinical practices confirm, the traditional Chinese medical science is for most of medicine of " strengthening the body resistance to consolidate the constitution ", as ginseng, Chinese caterpillar fungus, pilose antler etc. " help " class medicine, by modern research method, confirm, its Main Function brings into play by strengthening immunity of organisms and resistance the effect of preventing and curing diseases. between nutraceutical and immune system, close relationship is arranged, the mankind's feed directly affects immune normal operation, show after deliberation, body can strengthen by the fill-in that absorbs nourishment the immunocompetence of cell, guarantee the immune normal functions such as tissue and organ, thereby improve the immunity of human body.
And can effectively improve on the market food or the health medicine of immunity function, and effect is not very desirable, some also has side effect to health.
For the food or the health medicine that solve above raising immunity function, effect is undesirable, health is had to the problem of side effect, the invention provides a kind of effective, have no side effect there is the health food that strengthens immunity function.
A kind of health food with enhancing immunity function, the raw material weight proportioning is as follows:
Selenide of carragheen 1-10 part, Semen Coicis extract 50-150 part, dandelion extract 100-200 part, vitamin E 10-100 part;
In vitamin E, active principle content is 50%.
1.2 parts of selenide of carragheen, 100 parts of Semen Coicis extracts, 150 parts of dandelion extracts, 50 parts of vitamin Es.
A kind of health food with enhancing immunity function, the raw material weight proportioning is as follows:
Selenide of carragheen 1-10 part, Semen Coicis extract 50-150 part, dandelion extract 100-200 part, vitamin E 10-100 part, Semen euryales extract 1-40 part, Barley Greeg 50-150 part;
In vitamin E, active principle content is 50%.
Above-mentioned Semen Coicis extract, dandelion extract, Semen euryales extract are water extract.
1.2 parts of selenide of carragheen, 100 parts of Semen Coicis extracts, 150 parts of dandelion extracts, 50 parts of vitamin Es, 20 parts of Semen euryales extracts, 80 parts of Barley Greegs.
Described health food, make capsule, tablet or granule.
Health food of the present invention is to body weight, spleen, thymus gland immune organ weight are without obvious impact, this shows that health food of the present invention has the effect of direct enhancing cellular immunity and humoral immunity, rather than the volume and weight of the body weight by increasing mouse, immune organ is realized; Raw material type is few, the preparation method is simple; Health food of the present invention can strengthen immunity of organisms by the double action that improves Cellular Immunity and humoral immunity, for guaranteeing that body health, the generation that prevents disease and development have very important effect.
The specific embodiment
For a better understanding of the present invention, below in conjunction with specific embodiment, further illustrate.
embodiment 1:
A kind of health food with enhancing immunity function, the raw material weight proportioning is as follows:
1 part of selenide of carragheen, 150 parts of Semen Coicis extracts, 100 parts of dandelion extracts, 100 parts of vitamin Es;
Wherein in selenide of carragheen, the weight content of selenium is 2%;
In vitamin E, active principle content is 50%.
Make capsule, tablet or granule.
embodiment 2:
A kind of health food with enhancing immunity function, the raw material weight proportioning is as follows:
10 parts of selenide of carragheen, 50 parts of Semen Coicis extracts, 200 parts of dandelion extracts, 10 parts of vitamin Es;
Wherein in selenide of carragheen, the weight content of selenium is 2%;
In vitamin E, active principle content is 50%.
Make capsule, tablet or granule.
embodiment 3:
A kind of health food with enhancing immunity function, the raw material weight proportioning is as follows:
1.2 parts of selenide of carragheen, 100 parts of Semen Coicis extracts, 150 parts of dandelion extracts, 50 parts of vitamin Es.
Make capsule, tablet or granule.
embodiment 4:
A kind of health food with enhancing immunity function, the raw material weight proportioning is as follows:
1.2 parts of selenide of carragheen, 100 parts of Semen Coicis extracts, 150 parts of dandelion extracts, 50 parts of vitamin Es, 20 parts of Semen euryales extracts, 80 parts of Barley Greegs.
Make capsule, tablet or granule.
embodiment 5:
A kind of health food with enhancing immunity function, the raw material weight proportioning is as follows:
1 part of selenide of carragheen, 150 parts of Semen Coicis extracts, 100 parts of dandelion extracts, 100 parts of vitamin Es, 1 part of Semen euryales extract, 150 parts of Barley Greegs;
Wherein in selenide of carragheen, the weight content of selenium is 2%;
In vitamin E, active principle content is 50%.
Make capsule, tablet or granule.
embodiment 6:
A kind of health food with enhancing immunity function, the raw material weight proportioning is as follows:
10 parts of selenide of carragheen, 50 parts of Semen Coicis extracts, 200 parts of dandelion extracts, 10 parts of vitamin Es, 40 parts of Semen euryales extracts, 50 parts of Barley Greegs;
Wherein in selenide of carragheen, the weight content of selenium is 2%;
In vitamin E, active principle content is 50%.
Make capsule, tablet or granule.
the compliance test result experiment
1 materials and methods
1.1 sample
The capsule that embodiment 1-4 prepares, the 0.4g/ grain.
1.2 animal used as test grouping and tested material give mode
200 of the healthy Kunming mouses of clean level, body weight is 18~22g, Shandong University's Experimental Animal Center provides.
Get each 4.00g of capsule 's content that embodiment 1-4 prepares, respectively with soybean oil to 100mL, mix, wiring solution-forming, give respectively the animal subject gavage, 0.2mL/l0g body weight, every day, gavage was 1 time, and 5Od. recommends consumption according to human oral continuously, and animal used as test is divided 5 groups: 1 group of (1) embodiment; (2) embodiment is 2 groups; (3) embodiment is 3 groups; (4) embodiment is 4 groups; (5) control group (feed and raise isopyknic soybean oil).
1.3 experimental technique
1.3.1 the mouse spleen lymphocyte transformation experiment (mtt assay) that ConA induces
When experiment finishes, the aseptic spleen of getting, make cell suspension, and adjusting cell concentration is 3 * 10/mL, by " mtt assay ", detected. and lymphocytic multiplication capacity deducts with the OD value that adds the ConA hole OD value that does not add the ConA hole and means.
1.3.2 the mouse delayed allergy (DTH) that dinitrofluorobenzene is induced experiment (ear swelling method)
Before experiment finishes, 5d is by the mouse part skin about 3cm * 3cm scope of losing hair or feathers, evenly smear sensitization with 50 μ L DNFB solution, after 5d, l0 μ L DNFB evenly being applied in to the mouse right ear two sides is attacked, after 24h, mouse is put to death in the cervical vertebra dislocation, cut the left and right auricular concha, with card punch take off the 8mm diameter auricle, weigh, mean the degree of DTH with the difference of left and right ear weight.
1.3.3 antibody-producting cell detects (Jerne improves slide method)
Get the sheep blood of defiber, be made into 2% cell suspension, every mouse lumbar injection 0.2mL. puts to death the mouse of 5d after immunity, the aseptic spleen of getting, make cell suspension, 200 eye mesh screens filter, and cell are suspended in 5mL Hank ' s liquid after washing 2 times. by " improvement slide method ", detected, counting hemolysis plaque number, mean the antibody-producting cell number with plaque number/full splenocyte.
1.3.4 the mensuration of serum hemolysin (Hemagglutination Method)
Get the sheep blood of defiber, with physiological saline washing 3 times, be made into the cell suspension of 2% (v/v), O.2mL, after immune 5d, eye socket is got blood to every mouse lumbar injection, by standard " Hemagglutination Method ", is detected, and observes the hemagglutination degree. be calculated as follows the antibody product:
Antibody product=(s
l+ 2s
2+ 3s
3ns
n)
L, 2,3 in formula ... the index that n is the two-fold dilution, the rank that S is the aggegation degree.
1.3.5 mouse carbon is cleaned up experiment
Through the tail vein to injected in mice the india ink with 4 times of normal saline dilutions, every 10g body weight injection 0.1mL, timing immediately after prepared Chinese ink injects, after injecting prepared Chinese ink the 2nd, 1Omin, adjoin veniplex from intraocular respectively and get blood 2O μ L, joins 2mL0.1% Na
2c0
3solution, shake up. with Na
2c0
3solution is made blank, uses semi-automatic biochemical analyzer with 600nm wavelength photometry density value (OD).Mouse is put to death, get liver, spleen, dirty, weigh, be calculated as follows phagocytic index a.
K=(1gOD in formula
1-lg0D
2) (t
2-t
1)
1.3.6 Turnover of Mouse Peritoneal Macrophages is engulfed chicken red blood cell experiment (half endosome method)
To mouse peritoneal injection 2O% (v/v), with giving birth to the hematocrit chicken erythrocyte suspension that buries the salt solution preparation, every mouse lmL, interval 3min, mouse is put to death in the cervical vertebra dislocation, by " half endosome method ", is detected, and with following formula, calculates phagocytic rate and phagocytic index:
1.3.7 NK cytoactive detection (LDH determination method)
The dislocation of mouse cervical vertebra is put to death, and the aseptic spleen of getting, make splenocyte suspension, and adjusting cell concentration is 2 * 10
7/ mL, this is the effector cell. get well-grown YAC-1 cell, adjusting cell concentration is 4 * 10
5/ mL is as target cell. and detected by " LDH determination method ", with following formula, calculated the N cytoactive:
1.4 result is judged
Aspect cellular immune function, humoral immune function, monokaryon one macrophage function, NK cytoactive four, any two aspects positive as a result, can judge that this given the test agent has the enhancing immunity.
1.5 statistical procedures
Application SPSS statistical software carries out the variance analysis statistical disposition.
2 results
2.1 the impact on the mouse cell immunity
2.1.1 the impact on the mouse spleen lymphocyte conversion capability
The lymphocyte transformation ability of each embodiment group mouse is all higher than control group, and the difference of each embodiment group and control group all has statistical significance (in Table 1).
The impact (x ± s) of table 1 on the mouse spleen lymphocyte conversion capability
Group |
n |
Lymphocyte transformation ability (OD value) |
1 group of embodiment |
10 |
0.2053±0.1143
* |
2 groups of embodiment |
10 |
0.2397±0.0574
** |
3 groups of embodiment |
10 |
0.2805±0.0952
** |
4 groups of embodiment |
10 |
0.3123±0.0846
** |
Control group |
10 |
0.1025±0.0472 |
With control group, compare,
*p<0.05,
*p<0.01.
2.1.2 the impact on mouse delayed allergy (DTH)
The left and right auricle weight difference of each embodiment group mouse is all higher than control group, and has statistical significance (P<0.05) with the difference of control group, in Table 2.
The impact (x ± s) of table 2 on mouse delayed allergy (DTH)
Group |
n |
Left and right auricle weight difference (mg) |
1 group of embodiment |
10 |
9.08±2.08 |
2 groups of embodiment |
10 |
9.84±2.23 |
3 groups of embodiment |
10 |
10.96±0.54
* |
4 groups of embodiment |
10 |
12.13±0.38
* |
Control group |
10 |
7.11±2.42 |
With control group, compare,
*p<0.05.
2.2 the impact on mouse humoral immune
2.2.1 the impact on the antibody-producting cell number of mouse
The antibody-producting cell number of each embodiment group mouse is all higher than control group, and the difference of each embodiment group and negative control group all has statistical significance (in Table 3).
The impact (x ± s) of table 3 on the antibody-producting cell number of mouse
Group |
n |
Hemolysis plaque number (1*10/10
6) splenocyte
|
1 group of embodiment |
10 |
171.25±21.53
* |
2 groups of embodiment |
10 |
172.74±20.42
** |
3 groups of embodiment |
10 |
178.18±23.85
** |
4 groups of embodiment |
10 |
185.24±21.36
** |
Control group |
10 |
145.84±15.15 |
With control group, compare,
*p<0.05,
*p<0.01.
2.2.2 the impact on the mice serum hemolysin
The antibody product of each embodiment group mouse is all higher than control group, and has statistical significance (P<0.05) with the difference of negative control group, in Table 4.
The impact (x ± s) of table 4 on the mice serum hemolysin
Group |
n |
The antibody product |
1 group of embodiment |
10 |
155.6±17.7 |
2 groups of embodiment |
10 |
158.9±14.1 |
3 groups of embodiment |
10 |
167.4±24.2
* |
4 groups of embodiment |
10 |
178.2±20.5
* |
Control group |
10 |
148.4±22.2 |
With control group, compare,
*p<0.05.
2.3 the impact on the monocytes/macrophages phagocytic function of mouse
2.3.1 the impact that the monocytes/macrophages carbon of mouse is cleaned up
The phagocytic index a of each embodiment group mouse is all higher than control group, but the equal not statistically significant of difference of each embodiment group and control group (P > 0.05), in Table 5.
The impact (x ± s) that table 5 is cleaned up the monocytes/macrophages carbon of mouse
Group |
n |
Phagocytic index α |
1 group of embodiment |
10 |
6.142±0.848 |
2 groups of embodiment |
10 |
6.450±0.902 |
3 groups of embodiment |
10 |
6.507±1.084 |
4 groups of embodiment |
10 |
6.847±1.018 |
Control group |
10 |
5.664±0.799 |
2.3.2 the peritoneal macrophage of mouse is engulfed to the impact of chicken red blood cell ability
The peritoneal macrophage of each embodiment group mouse is to the phagocytic rate of chicken red blood cell and phagocytic index all higher than control group, but the equal not statistically significant of difference of each embodiment group and control group (P > 0.05), in Table 6.
Table 6 is engulfed the impact (x ± s) of chicken red blood cell ability on the peritoneal macrophage of mouse
Group |
n |
Phagocytic rate (%) |
The phagocytic rate data transaction |
Phagocytic index b |
1 group of embodiment |
10 |
18.04±4.35 |
0.435±0.054 |
0.225±0.064 |
2 groups of embodiment |
10 |
18.36±4.04 |
0.442±0.058 |
0.225±0.056 |
3 groups of embodiment |
10 |
19.01±4.17 |
0.458±0.049 |
0.233±0.058 |
4 groups of embodiment |
10 |
19.54±4.33 |
0.472±0.056 |
0.259±0.042 |
Control group |
10 |
17.11±3.46 |
0.421±0.050 |
0.196±0.048 |
2.4 the impact on the NK cytoactive of mouse
The NK cytoactive of each embodiment group mouse is all higher than control group, and 2,3,4 groups of embodiment wherein have statistical significance with the difference of control group, in Table 7.
The impact (x ± s) of table 7 on the NK cytoactive of mouse
Group |
n |
NK cytoactive (%) |
NK cytoactive data transaction |
1 group of embodiment |
10 |
34.42±4.75 |
0.620±0.051 |
2 groups of embodiment |
10 |
43.44±8.83 |
0.706±0.082
* |
3 groups of embodiment |
10 |
45.32±7.82 |
0.733±0.084
** |
4 groups of embodiment |
10 |
47.23±7.64 |
0.787±0.063
** |
Control group |
10 |
32.05±5.78 |
0.593±0.052 |
With control group, compare,
*p<0.05,
*p<0.01.
3 discuss
Detect health food of the present invention by mouse spleen lymphocyte transformation experiment and delayed allergy experiment and there is the effect that strengthens specific cellular immunity, health products prepared by embodiment 1-4 all can cause the raising of mouse spleen lymphocyte conversion capability, by causing that lymphocytic conversion capability strengthens, cause delayed allergy. clean up experiment by mouse carbon, peritoneal macrophage engulfs the chicken red blood cell experiment and the NK cytoactive detection detects health food of the present invention to the non-specific cell immunization, result shows that health food of the present invention is little on the impact of monokaryon one macrophage phagocytic function of mouse, but can improve the activity of NK cell, binding specificity cellular immunity detects.
In this research, we also measure by serum hemolysin and the antibody-producting cell number is measured the impact of detection health food of the present invention on humoral immunity, result shows, health food of the present invention can cause that the antibody-producting cell number of mouse increases, but serum hemolysin does not have significant change, embodiment 2, the degree that 3 health food antibody-producting cell numbers increase is obvious, and can cause that serum hemolysin improves. in test, our weighing the body weight of mouse, spleen, the thymus gland immune organ weight, detect the impact of health food of the present invention on Mouse Weight and immune organ weight, result shows that health food of the present invention is to body weight, spleen, the thymus gland immune organ weight is without obvious impact, this shows that health food of the present invention has the effect of direct enhancing cellular immunity and humoral immunity, rather than by increasing the body weight of mouse, the volume and weight of immune organ is realized.
In sum, health food of the present invention can strengthen immunity of organisms by the double action that improves Cellular Immunity and humoral immunity, for guaranteeing that body health, the generation that prevents disease and development have very important effect.Therefore, it being developed to food with health role or medicinal application is with a wide range of applications in clinical.