CN109790544A - 使用rna分子控制鞘翅目有害生物 - Google Patents

使用rna分子控制鞘翅目有害生物 Download PDF

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CN109790544A
CN109790544A CN201780046979.8A CN201780046979A CN109790544A CN 109790544 A CN109790544 A CN 109790544A CN 201780046979 A CN201780046979 A CN 201780046979A CN 109790544 A CN109790544 A CN 109790544A
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insect
diabroticaspp
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K·V·多诺霍
Y·瑙德特
P·菲尔德曼
L·德拉格夫
I·马莱特
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Abstract

本文披露了对鞘翅目昆虫具有毒性的双链RNA分子。具体地，本文提供了能够干扰有害生物靶基因并对靶有害生物具有毒性的干扰RNA分子。此外，本文披露了例如在转基因植物中制备并使用该干扰RNA或制备并使用该干扰RNA作为组合物中的活性成分以赋予免受昆虫损害的保护的方法。

Description

使用RNA分子控制鞘翅目有害生物
序列表
提供ASCII文本格式的序列表作为纸质副本的替代，该序列表是根据37C.F.R.§1.821提交的，名称为“81018_ST25.txt”，大小为548千字节，于2017年6月27日生成并经由EFS-Web提交。这个序列表特此通过引用以其披露内容并入本说明书中。
技术领域
本发明总体上涉及由其摄食活动而引起对作物植物损害的有害生物的控制，并且更具体地涉及通过包含干扰RNA分子的组合物控制鞘翅目有害生物。本发明进一步涉及包含干扰RNA分子的组合物并且涉及使用此类组合物的方法。
背景技术
根萤叶甲属(Diabrotica)中的昆虫种类(玉米根虫和黄瓜叶甲)被认为是对作物植物最重要的有害生物中的一些。例如，玉米根虫的种类，包括西方玉米根萤叶甲(Diabrotica virgifera virgifera)，西方玉米根虫(WCR)，巴氏根萤叶甲(D.barberi)，北方玉米根虫(NCR)，黄瓜十一星叶甲食根亚种(D.undecimpunctata howardi)，南方玉米根虫(SCR)及墨西哥玉米根萤叶甲(D.virgifera zeae)，墨西哥玉米根虫(MCR)，是北美最具破坏性的玉米有害生物，每年导致估计超过十亿美元的损失。西方玉米根虫也已经入侵欧洲并且每年导致估计五亿欧元损失。南美叶甲(除了别的之外，俗名包括叶甲、巴西小甲虫、葫芦甲虫及菊花甲虫)在南美是一种重要的玉米、大豆及花生有害生物。
玉米损失的大部分由根虫幼虫摄食导致。刚孵出的根虫幼虫位于土壤中的玉米根部并且最初开始摄食细根毛并钻入玉米植物的根尖中。随着幼虫长大，它们摄食初生根并挖隧道进入其中。当根虫数量巨大时，幼虫摄食和由根腐病病原体导致的受损根的劣变可以导致根减少至茎的基部。严重的根损伤干扰根将水和营养素运输进植物的能力，减少植物生长，并且导致谷物产量减少。严重的根损伤还可以导致玉米植物的倒伏，使得机械收获更困难或不可能。玉米根虫成虫主要摄食玉米须、花粉以及暴露的穗尖上的籽粒。如果玉米根虫成虫在玉米生殖组织存在之前开始出现，则成虫可以摄食叶组织，刮掉绿色的表面组织并且留下窗玻璃外观。成虫摄食穗丝可以导致穗尖处的穗丝减少，通常称作穗丝修剪(silk clipping)。在大田玉米中，甲虫种群在花粉散发过程中可以达到足以导致严重的穗丝修剪的程度，这可以干扰授粉并减少收率。因此，不像仅幼虫期导致损失的玉米的鳞翅目有害生物，玉米根虫的幼虫期和成虫期都能够对玉米导致经济损失。
根萤叶甲属昆虫有害生物主要是通过密集应用化学杀有害生物剂来控制，这些化学杀有害生物剂通过抑制昆虫生长、预防昆虫摄食或繁殖或导致死亡而可以有效对抗幼虫期和成虫期。由此可以达到良好的昆虫控制，但这些化学品有时也会影响其他益虫。另外的问题出现在使用高杀昆虫剂的区域中，在这些区域中玉米根虫甲虫种群对某些杀昆虫剂已经变得有抗性。通过各种抗性管理实践已部分地缓和了这种状况，但对于可替代的有害生物控制试剂存在着越来越多的需要。
来自苏云金芽孢杆菌(Bacillus thuringiensis)的若干天然Cry蛋白或工程化的Cry蛋白已经在转基因作物植物中表达并且在商业上被开发以控制某些鳞翅目和鞘翅目昆虫有害生物。例如，起始于2003年，通过表达Cry3Bb1、Cry34Ab1/Cry35Ab1、或修饰的Cry3A(mCry3A)或Cry3Ab(eCry3.1Ab)蛋白而控制玉米根虫的转基因玉米杂交种在美国已经是可商购的。
种子产业、大学研究员以及美国环境保护局已经一起合作来制定管理计划，以帮助缓解昆虫对表达杀昆虫蛋白的转基因植物的抗性的发生。它们主要是基于高剂量以及避护所策略。针对玉米的高剂量策略是使用表达甚至足够杀死部分抗性昆虫的高水平的杀昆虫蛋白(例如Cry蛋白)的玉米杂交种。基础的假设是杀死部分抗性的昆虫并且大力防止它们交配，从而延迟了抗性的产生。高剂量策略的成功部分取决于杀昆虫蛋白对具体昆虫种类的特异性活性以及多少该杀昆虫蛋白可以被表达于转基因玉米植物中。杀昆虫蛋白对有害生物的特异性活性越高，需要表达于转基因植物中以实现高剂量策略的杀昆虫蛋白的量越少。例如，表达鳞翅目-有效Cry蛋白(Cry1Ab)的玉米杂交种被认为对抗主要靶有害生物欧洲玉米螟(玉米螟)是高剂量的。由于Cry1Ab以LC50<10ng/cm2对于欧洲玉米螟幼虫很具毒性(即，高特异性活性)，在转基因植物中可实现的Cry1Ab表达水平很容易将这样的玉米杂交种置于高剂量范畴中。然而，不像鳞翅目-有效产品，当前根虫产品不被认为是高剂量的。它们表达的蛋白不有效对抗成虫并且对晚龄幼虫具有有限活性。因此，当前转基因根虫产品允许一些根虫幼虫存活并成为成虫。
因此，由玉米根虫成虫导致的穗丝修剪的经济水平仍可以甚至出现在种植为转基因玉米根虫杂交种的部分大田中。例如，西方玉米根虫成虫的密度可以在种植为转基因玉米根虫杂交种的部分大田中超过经济水平，这归因于甲虫的迁入以及成虫从转基因根系的直接羽化。已经有许多报道确认西方玉米根虫成虫羽化自某些玉米转基因根虫杂交种(Crowder等人2005.J.Econ.Entomol.[经济昆虫学杂志]98:534-551)。另一个出版物表明，当在大田中遇到一些转基因玉米植物或非转基因玉米植物时，西方玉米根虫成虫将展现出类似的摄食行为，并且表明转基因植物中的某些杀昆虫蛋白对可能影响抗性管理的成虫将具有显著效果是不太可能的。
因此，鉴定具有新作用模式的可替代的昆虫控制试剂将是有益的。特别有用的将可以是对靶昆虫有害生物的多个生活期具有毒性的新昆虫控制试剂。此类昆虫控制试剂可以包括靶向遗传因子(例如对于靶昆虫有害生物的生长和存活必不可少的基因)的那些昆虫控制试剂。
当一种生物体识别双链RNA(dsRNA)分子并水解它们时，RNA干扰(RNAi)发生。所产生的水解产物是约19-24个核苷酸长度的小RNA片段，这些小RNA片段称作小干扰RNA(siRNA)。然后这些siRNA扩散或被携带至整个生物体，包括越过细胞膜，在那里它们与mRNA(或其他的RNA)杂交并且导致RNA的水解。干扰RNA由RNA干扰沉默复合体(RISC)识别，RNA的效应链(或“引导链”)位于该复合体中。此引导链充当双链体序列的识别和破坏模板。每次siRNA与其互补RNA靶标杂交，该此过程都被重复，有效防止那些mRNA被翻译，并且因此“沉默”mRNA自其中转录的特异性基因的表达。大部分植物微小RNA(miRNA)对其靶mRNA显示出广泛的碱基配对，并引导其裂解(Jones-Rhoades等人(2006)Annu.Rev.PlantBiol.[植物生物学年评]57,19-53；Llave等人(2002)Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]97,13401-13406)。在其他例子中，干扰RNA可以结合至具有非完美互补性的靶RNA分子，导致在没有mRNA降解的情况下的翻译阻抑。到目前为止，多数研究的动物miRNA似乎以此方式发挥功能。
已发现RNAi可用于对某些昆虫有害生物的昆虫控制。RNAi策略典型地使用合成的、非天然存在的“干扰RNA”或“干扰RNA分子”，其典型地至少包含针对靶基因的RNA片段、间隔子序列和与第一RNA片段互补的第二RNA片段，从而可以形成双链RNA结构。这种非天然的双链RNA利用该昆虫中的天然RNAi途径来触发可能导致摄食和/或生长停止并且可能导致该昆虫有害生物的死亡的靶基因的下调。
尽管从文献已知聚焦于靶基因的RNAi策略可以在根萤叶甲属物种中产生杀昆虫效果，但还已知的是，不是每个靶序列都是成功的，并且不能预测杀昆虫效果。当用作dsRNA或siRNA时，绝大多数与玉米根虫DNA互补的序列在玉米根虫物种中不是致命的。例如，Baum等人((2007)Nature Biotechnology[自然生物技术]25:1322-1326)描述了通过RNAi抑制若干种WCR基因靶标的效果。这些作者报道，即使在超过520ng/cm2的非常高的iRNA(例如，dsRNA)浓度下，他们测试的26种靶基因中也有8种不能提供实验上显著的鞘翅目有害生物死亡率。另外，已知针对靶基因的dsRNA分子在一种昆虫物种中产生强RNAi效果，这样的靶基因可能不是针对不同昆虫物种的良好靶标。Whyard等人((2009)Insect Biochemistryand Molecular Biology[昆虫生物化学与分子生物学]39:824-832)报道了在四种不同昆虫物种中测试针对V-ATP酶基因的同种dsRNA分子时功效上有近100倍差异。
对于含有杀昆虫活性成分的组合物，以及对于使用此类组合物的方法，例如用于作物保护或昆虫介导的疾病的控制存在不断的需要。需要新颖的组合物来克服对现有杀昆虫剂的抗性问题和/或帮助缓解对现有转基因植物方法的抗性的发展。理想地，此类组合物具有高毒性并且当被靶有害生物口服摄取时是有效的并且对于使用对抗有害生物昆虫的幼虫期和成虫期两者而言具有适用性。因此，提供了其中这些特性中的任一种被增强的组合物的任何发明将代表本领域的进步。
发明内容
以上所列出的需要是通过本发明来满足的，在多个实施例中，本发明提供了控制经济上重要的昆虫有害生物的新方法。本发明部分地包含一种抑制一种或多种靶基因和蛋白质在鞘翅目昆虫有害生物中表达的方法。具体而言，本发明包含调节一种或多种靶基因在根萤叶甲属物种中表达的方法，如西方玉米根萤叶甲(西方玉米根虫)、巴氏根萤叶甲(北方玉米根虫)、黄瓜十一星叶甲食根亚种(南方玉米根虫)、墨西哥玉米根萤叶甲(墨西哥玉米根虫)、南美叶甲(Diabrotica speciosa)(菊花甲虫)以及相关物种，该方法导致摄食、生长、发育及繁殖的中止，并且最终导致昆虫的死亡。该方法包括将包含双链RNA(dsRNA)的干扰RNA分子或其修饰形式(如小干扰RNA(siRNA)序列)引入进有害生物昆虫体内的细胞或进入细胞外环境(如中肠)中，其中该dsRNA或siRNA进入这些细胞中，并且抑制至少一种或多种靶基因的表达，并且其中该一种或多种靶基因的抑制在该有害生物昆虫上发挥有害作用。该干扰RNA分子是非天然存在的。明确考虑的是，通过在该有害生物的饲料中提供一种或多种包含含有dsRNA或siRNA分子的干扰RNA分子的组合物，本发明的这些方法和组合物将在限制或消除任何植物中或其上的有害生物昆虫侵染方面是有用的。本发明还提供了干扰RNA分子，当递送至昆虫有害生物时，该干扰RNA分子通过毒性作用抑制该昆虫有害生物存活、生长、摄食和/或繁殖的能力，或限制有害生物相关的损害或作物植物的损失。这样的递送可以是通过在转基因植物(例如玉米)中产生干扰RNA，或通过将包含干扰RNA的组合物局部施用至植物或植物种子(如玉米植物或玉米种子)。递送可以进一步通过使昆虫与干扰RNA接触进行，如当昆虫摄食包含干扰RNA的植物材料时，因为该植物材料通过转基因方法正在表达干扰RNA，或者因为该植物材料涂覆有包含干扰RNA的组合物。该干扰RNA也可以以人工昆虫饲料提供，该昆虫然后通过摄食与其接触。该干扰RNA分子包含如下核苷酸序列，该核苷酸序列与可从该有害生物昆虫的靶基因转录的mRNA的核苷酸序列或可从该有害生物昆虫的靶基因转录的mRNA的核苷酸序列的一部分互补并且因此抑制该靶基因的表达，导致摄食、生长、发育、繁殖的中止，并且最终导致该有害生物昆虫的死亡。本发明进一步涉及至少包含或编码本发明的干扰RNA分子的一条链的片段的核酸构建体、核酸分子和重组载体。本发明还提供了嵌合核酸分子，这些嵌合核酸分子包含该干扰RNA的dsRNA的一条反义链，该反义链可操作地与植物微小RNA前体分子相关。本发明还提供了人工植物微小RNA前体，这些前体包含本发明的干扰RNA的dsRNA的一条反义链。
本发明进一步提供了一种干扰核糖核酸(RNA)分子，其中该RNA包含至少一种dsRNA，其中该dsRNA是包含退火的互补链的双链RNA的区域，其中的一条链包含具有至少19个连续核苷酸的序列，该序列与在根萤叶甲属靶基因中的靶核苷酸序列是至少部分互补的，并且(i)至少与SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ IDNO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19个连续核苷酸的片段，或其互补序列具有至少85％同一性；或者(ii)至少包含SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19个连续核苷酸的片段，或其互补序列；或者(iii)至少包含编码由SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400编码的氨基酸序列的核苷酸序列的19个连续核苷酸的片段，或其互补序列，其中该干扰RNA分子对鞘翅目植物有害生物具有杀昆虫活性。在一些实施例中，该干扰分子可以包括至少两种dsRNA，其中每种dsRNA包含核苷酸的序列，该序列与该靶基因内的靶核苷酸序列是至少部分互补的。在进一步的实施例中，每种dsRNA可以包含不同的核苷酸序列，该序列与该靶基因中的不同靶核苷酸序列是互补的。
本发明进一步提供了如下组合物，这些组合物包含本发明的一种或多种干扰RNA分子，这些干扰RNA分子包含两种或更多种dsRNA分子，其中这两种或更多种RNA分子各自包含不同的反义链，或包含两种或更多种核酸构建体或核酸分子或人工植物微小RNA前体。
本发明进一步提供了用于抑制鞘翅目昆虫基因表达的杀昆虫组合物，该组合物包含本发明的dsRNA，以及农业上可接受的载体。在一个实施例中，在此所述的根萤叶甲属基因表达的抑制引起摄食和生长的中止，并且最终导致该根萤叶甲属昆虫的死亡。
本发明进一步涉及转基因植物，这些转基因植物产生一种或多种本发明的干扰RNA分子，这些干扰RNA分子自我保护免受昆虫摄食损害，并且涉及单独使用或与其他昆虫控制策略组合使用这些植物以赋予最大昆虫控制能力的方法。产生一种或多种本发明的干扰RNA分子或用包含一种或多种本发明的干扰RNA分子的组合物处理的植物和/或植物部分对昆虫有害生物侵染是高度抗性的。例如，经济上重要的鞘翅目有害生物可以通过产生本发明的干扰RNA分子的植物或通过用一种包含本发明的干扰RNA分子的组合物处理的植物或植物种子加以控制。
本发明还提供了一种控制鞘翅目昆虫植物有害生物的方法，该方法包括使该鞘翅目昆虫与一种作为本发明的干扰RNA或能够产生本发明的干扰RNA的核酸分子接触，该干扰RNA用于抑制基因在该鞘翅目昆虫中的表达，从而控制该鞘翅目昆虫。
在其他方面中，本发明提供了一种减少转基因植物上的根萤叶甲属昆虫种群的方法，该转基因植物表达第二杀昆虫剂，例如杀昆虫蛋白(除了一种能够抑制靶基因在根萤叶甲属昆虫中表达的本发明的干扰RNA之外)，从而减少该根萤叶甲属昆虫种群。该第二杀昆虫剂可以是源自苏云金芽孢杆菌的杀昆虫蛋白。苏云金芽孢杆菌杀昆虫蛋白可以是许多杀昆虫蛋白中的任何一种，包括但不限于Cry1蛋白、Cry3蛋白、Cry7蛋白、Cry8蛋白、Cry11蛋白、Cry22蛋白、Cry23蛋白、Cry36蛋白、Cry37蛋白、Cry34蛋白连同Cry35蛋白、二元杀昆虫蛋白CryET33和CryET34、二元杀昆虫蛋白TIC100和TIC101、二元杀昆虫蛋白PS149B1、VIP、TIC900或相关蛋白、TIC901、TIC1201、TIC407、TIC417、修饰的Cry3A蛋白、或杂合蛋白或由任何前述杀昆虫蛋白制成的嵌合体。在其他实施例中，该苏云金芽孢杆菌杀昆虫蛋白选自下组，该组由以下组成：Cry3Bb1、Cry34Ab1连同Cry35Ab1、mCry3A以及eCry3.1Ab。
在其他实施例中，该第二杀昆虫剂可以源自除苏云金芽孢杆菌外的其他来源。该第二杀昆虫剂可以是选自下组的试剂，该组包含：马铃薯糖蛋白、蛋白酶、蛋白酶抑制剂、脲酶、α-淀粉酶抑制剂、成孔蛋白、几丁质酶、凝集素、工程化抗体或抗体片段、蜡样芽孢杆菌(Bacillus cereus)杀昆虫蛋白、致病杆菌属(Xenorhabdus spp.)(如嗜线虫致病杆菌(X.nematophila)或伯氏致病杆菌(X.bovienii))杀昆虫蛋白、光杆菌属(Photorhabdusspp.)(如发光杆菌(P.luminescens)或非共生光杆菌(P.asymobiotica))杀昆虫蛋白、侧孢短芽孢杆菌(Brevibacillus laterosporous)杀昆虫蛋白、球形赖氨酸芽胞杆菌(Lysinibacillus sphearicus)杀昆虫蛋白、色杆菌属(Chromobacterium spp.)杀昆虫蛋白、噬虫霉耶尔森氏菌杀昆虫蛋白、乳状芽孢杆菌(Paenibacillus popiliae)杀昆虫蛋白、梭菌属(Clostridium spp.)(如双酶梭菌(C.bifermentans))杀昆虫蛋白、产碱菌属(Alcaligenes spp.)杀昆虫蛋白、假单胞菌属(Pseudomonas spp.)杀昆虫蛋白、和木质素。在其他实施例中，该第二试剂可以是至少一种源自来自发光杆菌、致病杆菌、沙雷氏菌或耶尔森氏菌的杀昆虫毒素复合物(Tc)的杀昆虫蛋白。在其他实施例中，该杀昆虫蛋白可以是源自杀昆虫细菌(如光杆菌属)的ADP-核糖基转移酶。在其他实施例中，该杀昆虫蛋白可以是VIP蛋白，如来自蜡样芽孢杆菌的VIP1或VIP2。在仍其他实施例中，该杀昆虫蛋白可以是源自杀昆虫细菌的二元毒素，如来自侧孢芽孢杆菌的ISP1A和ISP2A或来自球形赖氨酸芽胞杆菌的BinA和BinB。在仍其他实施例中，该杀昆虫蛋白可以是工程化的或可以是任何前述杀昆虫蛋白的杂合体或嵌合体。
在其他方面中，本发明提供了一种减少对本发明的干扰RNA的抗性在根萤叶甲属昆虫种群中的发展的方法，该方法包括在被该根萤叶甲属昆虫种群摄食的转基因植物中表达本发明的干扰RNA，该干扰RNA能够抑制靶基因在幼虫和成虫根萤叶甲属昆虫中的表达，从而与暴露于仅能够抑制在此描述的根萤叶甲属基因在根萤叶甲属昆虫的幼虫期或成虫期的表达的干扰RNA的根萤叶甲属昆虫种群相比，减少抗性在该根萤叶甲属昆虫种群中的发展。
在其他方面中，本发明提供了一种降低可从在此描述的根萤叶甲属基因转录的靶RNA在根萤叶甲属昆虫中的水平的方法，该方法包括使该根萤叶甲属昆虫与一种包含本发明的干扰RNA分子的组合物接触，其中该干扰RNA分子降低该靶RNA在根萤叶甲属昆虫的细胞中的水平。
在仍其他方面中，本发明提供了一种赋予植物或其部分对根萤叶甲属昆虫耐受性或鞘翅目植物有害生物耐受性的方法，该方法包括向该植物或其部分中引入本发明的干扰RNA分子、dsRNA分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子和/或组合物，从而赋予该植物或其部分对该根萤叶甲属昆虫或鞘翅目植物有害生物的耐受性。
在另外的方面中，本发明通过了一种减少被根萤叶甲属昆虫摄食的植物的根损害的方法，该方法包括向该植物的细胞中引入本发明的干扰RNA分子、dsRNA、核酸分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子和/或组合物，从而减少被根萤叶甲属昆虫摄食的植物的根损害。
在其他方面中，本发明提供了一种产生对鞘翅目昆虫具有毒性的转基因植物细胞的方法，该方法包括向植物细胞中引入本发明的干扰RNA分子、dsRNA、核酸分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子和/或组合物，从而产生与对照植物细胞相比，对该鞘翅目昆虫具有毒性的转基因植物细胞。
在另外的方面中，本发明提供了一种产生对鞘翅目昆虫摄食损害具有增强的耐受性的转基因植物的方法，该方法包括向植物中引入本发明的干扰RNA分子、dsRNA、核酸分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子和/或组合物，从而产生与对照植物相比，对鞘翅目昆虫摄食损害具有增强的耐受性的转基因植物。
在其他方面中，本发明提供了一种增强鞘翅目昆虫种群的控制的方法，该方法包括提供本发明的转基因植物或转基因种子并且向该转基因植物或该转基因种子施用一种对鞘翅目昆虫而言具有杀昆虫性的化学杀有害生物剂，从而增强该鞘翅目昆虫种群的控制。
在其他方面中，本发明提供了一种为玉米种植者提供将玉米作物中的鞘翅目昆虫有害生物种群控制在低于经济阈值之下的手段的方法，该方法包括(a)向该种植者销售或为其提供包含本发明的dsRNA、核酸分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子和/或组合物的转基因玉米种子；并且(b)向该种植者做广告宣传该转基因玉米种子产生能够控制鞘翅目昆虫有害生物种群的转基因玉米植物。
在另一方面，本发明提供了一种鉴定直系同源靶基因用作控制植物有害生物的RNAi策略的方法，所述方法包括以下步骤：a)产生将扩增选自下组的靶标的引物对，该组包含SEQ ID NO:31-90、397、398，或其互补序列，或由其组成；b)扩增来自该植物有害生物的核酸样品的直系同源靶基因；c)鉴定直系同源靶基因的序列；d)产生干扰RNA分子，其中该RNA包含至少一种dsRNA，其中该dsRNA是包含退火的互补链的双链RNA的区域，其中的一条链包含具有至少19个连续核苷酸的序列，该序列与该靶基因中的直系同源靶核苷酸序列是至少部分互补的；以及e)确定步骤(d)的干扰RNA分子是否对该植物有害生物具有杀昆虫活性。如果该干扰RNA对该植物有害生物靶基因具有杀昆虫活性，则已鉴定出用于控制植物有害生物的直系同源靶基因。
在下文本发明的描述中更详细地阐述本发明的这些方面和其他方面。
序列表中的序列简述
SEQ ID NO:1-30是用于合成干扰RNA分子以测试杀昆虫活性的DNA编码序列的片段。
SEQ ID NO:31-90和397-398是用于鉴定来自根萤叶甲属的靶基因的引物的核酸序列，使用RNAi策略将这些靶基因用于测试杀昆虫活性。
SEQ ID NO:91-120是在基于RNAi的杀昆虫活性筛选中鉴定的30种靶基因的完整DNA编码序列。
SEQ ID NO:121-150是用于合成干扰RNA分子以测试杀昆虫活性的DNA编码序列的片段的RNA序列。
SEQ ID NO:151-180是在基于RNAi的杀昆虫活性筛选中鉴定的30种靶基因的完整DNA编码序列的RNA序列。
SEQ ID NO:181-210是在基于RNAi的杀昆虫活性筛选中鉴定的30种靶基因的完整mRNA序列，包括5’和3’UTR。
SEQ ID NO:211-240是在基于RNAi的杀昆虫活性筛选中鉴定的30种靶基因的完整DNA编码序列的反义RNA序列。
SEQ ID NO:241-270是由在基于RNAi的杀昆虫活性筛选中鉴定的30种靶基因编码的蛋白质的氨基酸序列。
SEQ ID NO:271-273是在基于RNAi的杀昆虫活性筛选中鉴定的三种选择的WCR靶基因(BPA_15366、BPA_71568和BPA_16830)的NCR直系同源物的DNA编码序列。
SEQ ID NO:274-276是在基于RNAi的杀昆虫活性筛选中鉴定的三种选择的WCR靶基因(BPA_15366、BPA_71568和BPA_16830)的NCR直系同源物的DNA编码序列的RNA序列。
SEQ ID NO:277-279是在基于RNAi的杀昆虫活性筛选中鉴定的三种选择的WCR靶基因(BPA_15366、BPA_71568和BPA_16830)的SCR直系同源物的DNA编码序列。
SEQ ID NO:280-282是在基于RNAi的杀昆虫活性筛选中鉴定的三种选择的WCR靶基因(BPA_15366、BPA_71568和BPA_16830)的SCR直系同源物的DNA编码序列的RNA序列。
SEQ ID NO:283-287和399-400是BPA_15366靶基因片段的DNA序列。
SEQ ID NO:288-293是BPA_71568靶基因片段的DNA序列。
SEQ ID NO:294-297是BPA_16830靶基因片段的DNA序列。
SEQ ID NO:298-302和401-402是BPA_15366靶基因mRNA片段的RNA序列。
SEQ ID NO:303-308是BPA_71568靶基因mRNA片段的RNA序列。
SEQ ID NO:309-312是BPA_16830靶基因mRNA片段的RNA序列。
SEQ ID NO:313-317是BPA_15366靶基因片段的DNA序列。
SEQ ID NO:318-322是与GFP DNA序列可操作连接的BPA_15366靶基因片段的DNA序列。
SEQ ID NO:323-344是在5’和3’端与GFP“填充物”DNA序列可操作连接的BPA_15366靶基因片段的DNA序列。
SEQ ID NO:345-349是与GFP RNA序列可操作连接的BPA_15366靶基因mRNA片段的RNA序列。
SEQ ID NO:350-371是在5’和3’端与GFP“填充物”DNA序列可操作连接的BPA_15366靶基因片段的RNA序列。
SEQ ID NO:372是BPA_16372靶基因的编码序列的核酸序列。
SEQ ID NO:373是SEQ ID NO:372的RNA序列。
SEQ ID NO:374是BPA_16372靶基因的mRNA序列，包括5’和3’UTR。
SEQ ID NO:375是SEQ ID NO:372的反义RNA序列。
SEQ ID NO:376是由SEQ ID NO:372编码的蛋白质的氨基酸序列。
SEQ ID NO:377是BPA_16372靶基因的NCR直系同源物的核酸编码序列。
SEQ ID NO:378是SEQ ID NO:377的RNA序列。
SEQ ID NO:379是BPA_16372靶基因的SCR直系同源物的核酸编码序列。
SEQ ID NO:380是SEQ ID NO:379的RNA序列。
SEQ ID NO:381-388是BPA_16372靶基因片段的DNA序列。
SEQ ID NO:389-396是BPA_16372靶基因mRNA片段的RNA序列。
SEQ ID NO:403是编码靶基因的发夹RNA结构的DNA序列。
具体实施方式
下面提供了本发明的详细说明，以协助本领域的普通技术人员实践本发明。本说明不旨在是可以实施本发明的所有不同方式，或可以添加到本发明中的所有特征的详细目录。例如，关于一个实施例所说明的特征可以并入其他实施例中，并且关于具体实施例所说明的特征可以从那个实施例删除。另外，鉴于本披露内容，对本发明的不同实施例的众多变体以及附加对于本领域技术人员是明显的，这不脱离本发明。因此，以下说明旨在阐述本发明的一些具体实施例，并且并没有穷尽地叙述其所有排列、组合和变化。本领域的普通技术人员将意识到，在在此描述的这些实施例中可以做出修饰和变化而不偏离本发明的精神或范围。
除非另外定义，在此所使用的所有技术和科学术语均具有与本发明所属领域的普通技术人员通常所理解的相同的含义。在此本发明的说明中使用的术语是仅出于描述具体实施例的目的，且并不旨在限制本发明。在此提及的所有出版物、专利申请、专利以及其他参考文献通过引用以其全文结合在此。
为了清晰起见，定义了在本说明书中所使用的某些术语并且将其呈现如下：
如在此使用的，“一个(a)”、“一个(an)”或“该(the)”可以意指一个或多于一个。例如，细胞可以意指单个细胞或多个细胞。
如在此使用的，“和/或”是指并且涵盖一个或多个相关的列出项的任何及全部可能组合，连同当以可替代性(或)解释时组合的缺少。
另外，当指可计量值(例如化合物或试剂、剂量、时间、温度等等的量)时，如在此使用的，术语“约(about)”意图包括所指值的±20％、±10％、±5％、±1％、±0.5％、或甚至±0.1％的变化。
如在此使用的，过渡短语“基本上由……组成”是指一项权利要求的范围将被解释为包括该权利要求中所提到的指定材料或步骤以及不实质上影响要求保护的发明的一个或多个基本特征和新特征的那些材料或步骤。因此，当用于本发明的权利要求中时，术语“基本上由……组成”并不意在被解释为等同于“包含(comprising)”。“编码序列”是转录成RNA(如mRNA、rRNA、tRNA、snRNA、正义RNA或反义RNA)的核酸序列。优选地，RNA进而在生物中被翻译以产生蛋白质。
术语核苷酸或氨基酸序列的“序列相似性”或“序列同一性”意指两个或更多个序列的同一性或相似性程度，并且可以常规地通过使用已知软件或计算机程序(如Best-Fit或Gap成对比较程序(GCG威斯康星州软件包(Wisconsin Package)，遗传学计算机集团公司(Genetics Computer Group)，科学先驱路575号(575Science Drive)，麦迪逊，威斯康星州，53711))确定。BestFit使用Smith和Waterman，Advances in Applied Mathematics[应用数学进展]2:482-489(1981)的局部同源性算法以找到两个序列之间最佳的同一性或相似性片段。通常通过在比较窗口上比较这两个序列的部分来进行两个或更多个多核苷酸或多肽之间的序列比较，以鉴定并比较序列相似性的局部区域。比较窗口通常是从约20至200个连续核苷酸。Gap使用Needleman和Wunsch,J.Mol.Biol.[分子生物学杂志]48:443-453(1970)的方法执行所有一个序列与所有另一个相似的序列的全局比对。当使用序列比对程序(如BestFit)来确定DNA序列同源性、相似性或同一性的程度时，可以使用默认设置，或者可以选择适当的评分矩阵以优化同一性、相似性或同源性评分。类似地，当使用程序(如BestFit)来确定两个不同氨基酸序列之间的序列同一性、相似性或同源性时，可以使用默认设置，或者可以选择适当的评分矩阵(如blosum45或blosum80)以优化同一性、相似性或同源性评分。
在两个核酸或两个氨基酸序列的上下文中，短语“基本上同一的”是指当针对最大对应性进行比较和比对时具有至少约50％核苷酸或氨基酸残基同一性的两个或更多个序列或子序列，如使用以下序列比较算法之一或通过目测检查所测量的。在某些实施例中，基本上同一的序列具有至少约60％、或至少约70％、或至少约80％、或甚至至少约90％或95％核苷酸或氨基酸残基同一性。在某些实施例中，基本上的同一性存在于这些序列的整个长度为至少约50个残基的区域中，或在整个至少约100个残基的区域中，或这些序列在至少约150个残基中是基本上同一的。在另外的实施例中，当这些序列在编码区的整个长度上时，这些序列是基本上同一的。
在本发明的上下文中，术语“同源性”是指在两个核酸或氨基酸序列之间就核苷酸和氨基酸同一性或相似性而言的相似性水平(序列相似性或同一性)。同源性、同源物、以及同源的也是指不同核酸或蛋白质之间相似功能特性的概念。同源物包括直系同源物和旁系同源物的基因。可以通过使用在此公开的编码序列或在适当的数据库中找到的同源物(例如在NCBI或其他数据库中)，以一种或多种以下方式确定同源物。对于氨基酸序列，应使用算法比较序列(例如参见“同一性”和“基本上的同一性”部分)。对于核苷酸序列，可以用几乎相同的方式将一个DNA分子的序列与已知或推定的同源物的序列进行比较。跨越分子的任何实质区域(DNA、RNA、或蛋白质分子)上，同源物是至少20％同一性、或至少30％同一性、或至少40％同一性、或至少50％同一性、或至少60％同一性、或至少70％同一性、或至少80％同一性、或至少88％同一性、或至少90％同一性、或至少92％同一性、或至少95％同一性。
用于比较的序列的最佳比对可以按照以下方式进行，例如通过Smith和Waterman,Adv.Appl.Math.[应用数学进展]2:482(1981)的局部同源性算法、通过Needleman和Wunsch,J.Mol.Biol.[分子生物学杂志]48:443(1970)的同源比对算法、通过Pearson和Lipman,Proc.Nat'l.Acad Sci.USA[美国国家科学院院刊]85:2444(1988)的相似性方法的搜索，通过这些算法的计算机化实施(威斯康星州遗传学分析软件包中的GAP、BESTFIT、FASTA和TFASTA，遗传学计算机集团公司(Genetics Computer Group)，科学先驱路575号(575Science Dr.)，麦迪逊，威斯康星州)，或通过目测检查(总体上参见Ausubel等人，下文)。
适合于确定序列同一性百分比以及序列相似性的算法的一个实例是BLAST算法，它描述于以下文献中：Altschul等人,J.Mol.Biol.[分子生物学杂志]215:403-410(1990)。执行BLAST分析的软件可通过美国国家生物技术信息中心(National Center forBiotechnology Information)公开地获得(http://www.ncbi.nlm.nih.gov/)。这种算法涉及首先通过鉴定查询序列中具有长度W的短字码而鉴定得分高的序列对(HSP)，这些得分高的序列对当与数据库序列中具有相同长度的字码(word)进行比对时匹配或满足一些正-值阈值的得分T。T被称为邻近字码得分阈值(Altschul等人，1990)。这些初始的邻近字码命中充当种子用于起始搜索以发现包含它们的较长的HSP。然后，将这些字码命中在两个方向上沿着每个序列延伸直到累积的比对得分可以增加。对于核苷酸序列，使用参数M(对于一对匹配残基的奖赏得分；总是>0)和N(对于错配残基的罚分；总是<0)来计算累积的得分。对于氨基酸序列，使用得分矩阵来计算累积得分。当累积的比对得分从它的最大达到值降低了数量X；由于累积一个或多个负得分的残基比对使累积得分趋于零或零以下；或者到达任一序列的末端时，停止这些字码命中在每个方向上的延伸。BLAST算法的参数W、T、以及X决定了比对的灵敏度与速度。BLASTN程序(对于核苷酸序列)使用字长(W)为11、期望值(E)为10、截止值(cutoff)为100、M＝5、N＝-4、以及两条链的比较作为默认值。对于氨基酸序列，BLASTP程序使用字长(W)为3、期望值(E)为10、以及BLOSUM62评分矩阵作为默认值(参见Henikoff和Henikoff,Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]89:10915(1989))。
除了计算序列同一-性百分数之外，BLAST算法还进行两个序列之间相似性的统计分析(参见，例如Karlin和Altschul,Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]90:5873-5787(1993))。由BLAST算法提供的相似性的一种量度是最小概率总和(P(N))，它提供了在两个核苷酸或氨基酸序列之间会偶然发生匹配的概率的指示。例如，若在测试核酸序列与参考核酸序列的比较中最小概率总和小于约0.1、更优选地小于约0.01、并且最优选地小于约0.001，则该测试核酸序列被认为是与该参考序列相类似的。
用于进行序列比对的另一种被广泛使用和接受的计算机程序是CLUSTALW v1.6(Thompson等人Nuc.Acids Res.[核酸研究]22:4673-4680,1994)。将匹配的碱基或氨基酸的数目除以碱基或氨基酸的总数目并乘以100，获得百分比同一性。例如，如果两个580个碱基对序列具有145个匹配的碱基，则它们会是25％同一的。如果两个进行比较的序列具有不同的长度，则将匹配的数目除以这两个长度中较短的一个。例如，如果在200个氨基酸的蛋白质与400个氨基酸的蛋白质之间存在100个匹配的氨基酸，则相对于较短的序列这两个蛋白质是50％同一的。如果该较短的序列在长度上小于150个碱基或50个氨基酸，则将匹配的数目除以150(对于核酸碱基而言)或50(对于氨基酸而言)并乘以100，获得百分比同一性。
当两个核苷酸序列在严格条件下彼此杂交时，这两个核苷酸序列也可以被认为是基本上同一的。在代表性实施例中，被认为基本上同一的两个核苷酸序列在高严格条件下彼此杂交。
术语“严格条件”或“严格杂交条件”包括指多核苷酸与其靶序列杂交的程度将比它与其他序列杂交的程度可检测地更高(例如，至少2倍于背景)的条件。严格条件是序列依赖性的并且在不同的情形下将会不同。通过控制该杂交和/或洗涤条件的严格度，能够鉴定与该探针(同源探测)100％互补的靶多核苷酸。可替代地，可调整严格条件以允许序列中的一些错配，这样使得检测到较低程度的相似性(异源探测)。典型地，严格条件将是这些：其中盐浓度小于约1.5M Na离子、典型地为约0.01至1.0M Na离子(或其他盐)，pH为7.0至8.3，并且对于短探针(例如，10至50个核苷酸)温度为至少30℃而对于长探针(例如，大于50个核苷酸)至少约60℃。还可以通过添加去稳定剂(如甲酰胺)来达到严格条件。示例性低严格条件包括在37℃下使用30％至35％甲酰胺、1M NaCl、1％SDS(w/v；十二烷基硫酸钠)的缓冲溶液进行杂交，并且在50℃至55℃下在1×至2×SSC(20×SSC＝3.0M NaCl/0.3M柠檬酸三钠)中洗涤。中等严格条件检测共享至少80％序列同一性的序列。示例性中等严格条件包括在37℃下在40％至45％甲酰胺、1M NaCl、1％SDS中杂交，并且在55℃至60℃下在0.5×至1×SSC中洗涤。高严格条件检测共享至少90％序列同一性的序列。示例性高严格条件包括在37℃下在50％甲酰胺、1M NaCl、1％SDS中杂交，并且在60℃至65℃下在0.1×SSC中洗涤。特异性通常取决于杂交后的洗涤，关键因素是最终洗涤溶液的离子强度以及温度。对于DNA-DNA杂交体，可从Meinkoth以及Wahl(Anal.Biochem.[分析生物化学],138:267-284,1984)的方程粗略估计Tm：Tm＝81.5℃+16.6(log M)+0.41(％GC)-0.61(％form)-500/L；其中M是一价阳离子的摩尔浓度，％GC是DNA中的鸟苷以及胞嘧啶核苷酸的百分比，％form是杂交溶液中甲酰胺的百分比，并且L是杂交体的长度(单位为碱基对)。Tm是50％的互补靶序列与完美匹配的探针杂交时的温度(在限定的离子强度及pH下)。对于每1％错配，Tm降低约1℃；因此，可调整Tm、杂交和/或洗涤条件以便杂交至所希望的同一性的序列。例如，如果寻找具有大约90％同一性的序列，则可将Tm降低10℃。一般来说，将严格条件选择为比特定序列及其互补序列在限定的离子强度以及pH下的热熔点(Tm)低约5℃。然而，极严格条件可利用低于热熔点(Tm)1℃、2℃、3℃或4℃的杂交和/或洗涤；中等严格条件可利用低于热熔点(Tm)6℃、7℃、8℃、9℃或10℃的杂交和/或洗涤；低严格条件可利用低于热熔点(Tm)11℃、12℃、13℃、14℃、15℃或20℃的杂交和/或洗涤。使用该方程、杂交和洗涤组成以及所希望的Tm，本领域普通技术人员应了解，杂交和/或洗涤溶液严格性的变化固有地得到了描述。如果所希望的错配程度导致Tm小于45℃(水溶液)或32℃(甲酰胺溶液)，则优选增加SSC浓度以使得可使用较高温度。有关核酸杂交的详尽指南见于以下文献：Tijssen,Laboratory Techniquesin Biochemistry and Molecular Biology—Hybridization with Nucleic Acid Probes[生物化学与分子生物学实验室技术－与核酸探针杂交],第I部分,第2章“Overview ofprinciples of hybridization and the strategy of nucleic acid probe assays[杂交原理概述和核酸探针测定策略]”,Elsevier(爱思唯尔),纽约(1993)；以及CurrentProtocols in Molecular Biology[现代分子生物学实验技术],第2章,Ausubel等人编著,Greene Publishing与Wiley-Interscience,纽约(1995)。严格杂交的方法是本领域已知的，其条件可以通过本领域已知的方法计算。这披露于Sambrook等人,Molecular Cloning:A Laboratory Manual[分子克隆：实验室手册]，第二版,Cold Spring Harbor LaboratoryPress[冷泉港实验室出版社],1989,Cold Spring Harbor[冷泉港],纽约以及CurrentProtocols in Molecular Biology[现代分子生物学实验技术],Ausebel等人(编),约翰·威利父子公司(John Wiley and Sons,Inc.),2000中。确定序列同一性百分比的方法是本领域已知的，其实例是GCG计算机序列分析软件(GCG公司，麦迪逊，威斯康星州)。
如果在严格条件下彼此不杂交的核酸所编码的蛋白质是基本上同一的，则它们仍然是基本上同一的(例如，由于遗传密码的简并性)。
两个核酸序列或蛋白质基本上一致的另一个指示是由第一核酸编码的蛋白质与由第二核酸编码的蛋白质进行免疫性交联反应。因此，蛋白质典型地是与第二蛋白质基本上同一的，例如其中这两种蛋白质仅区别于保守性置换。
当核酸序列编码了多肽(该多肽与由参考核酸序列所编码的多肽具有相同的氨基酸序列)时，这种核苷酸序列与这种参考核酸序列“同类编码”。
如在此使用的，“互补”多核苷酸是能够根据标准Watson-Crick互补性规则发生碱基配的那些。具体而言，嘌呤将与嘧啶进行碱基配对，以便形成鸟嘌呤与胞嘧啶配对(G:C)和在DNA情况下腺嘌呤与胸腺嘧啶配对(A:T)或在RNA情况下腺嘌呤与尿嘧啶配对(A:U)的组合。例如，序列“A-G-T”结合至互补序列“T-C-A”。应当理解两种多核苷酸可以相互杂交，甚至它们彼此并不完全互补，其条件是每种多核苷酸具有至少一个与另一种多核苷酸基本上互补的区域。
术语“互补的(complementary)”或“互补性(complementarity)”是指多核苷酸在容许性盐条件和温度条件下通过碱基配对发生天然结合。两单链分子之间的互补性可以是“部分的(partial)”，其中这些核苷酸中仅一些结合，或者当这些单链分子之间存在完全互补性时，这种互补性可以是完全的。核酸链之间的互补性程度对于核酸链之间杂交的效率和强度具有显著影响。
如在此使用的，术语“基本上互补”或“部分互补的”意指两个核酸序列在其至少约50％、60％、70％、80％或90％的核苷酸处是互补的。在一些实施例中，这两个核酸序列可以至少在其85％、90％、95％、96％、97％、98％、99％或更多的核苷酸处是互补的。术语“基本上互补的”和“部分互补的”也可以意指两个核酸序列可以在高严格性条件下杂交并且这类条件是本领域熟知的。
如在此使用的，“dsRNA”或“RNAi”是指由单个自身互补RNA链或至少由两条互补RNA链形成的多核糖核苷酸结构。互补程度(换言之，％同一性)不需要必须是100％。而是，它必须足够允许在所采用的条件下形成双链结构。如在此使用的，术语“完全地互补”意指dsRNA的核苷酸序列的所有碱基都与靶核苷酸序列的碱基互补或‘匹配’。术语“至少部分地互补”意指dsRNA的碱基与靶核苷酸序列的碱基之间存在小于100％的匹配。本领域的技术人员将理解，为了介导靶基因表达的下调，dsRNA仅需要与靶核苷酸序列至少部分地互补。本领域已知，相对于靶序列而具有插入、缺失以及错配的RNA序列在RNAi方面仍然可以有效。根据本发明，优选地是，dsRNA和靶基因的靶核苷酸序列共有至少80％或85％的序列同一性，优选至少90％或95％序列同一性，或更优选至少97％或98％序列同一性，并且再更优选至少99％序列同一性。可替代地，在24个部分互补的核苷酸的每个长度上，如与靶核苷酸序列相比，dsRNA可以包含1、2、或3个错配。本领域的普通技术人员将了解，dsRNA与靶核苷酸序列之间共有的互补性程度可以取决于有待下调的靶基因或者取决于基因表达有待控制的昆虫有害生物物种而改变。
应了解的是，dsRNA可以包含双链RNA的区域，该双链RNA包含退火的互补链，其中的一条链，即正义链，包含与靶基因内的靶核苷酸序列至少部分互补的核苷酸序列。
靶核苷酸序列可以选自靶基因或其RNA转录物的任何合适区域或核苷酸序列。举例来说，靶核苷酸序列可以位于靶基因或RNA转录物的5'UTR或3'UTR内，或者基因的外显子或内含子区域内。本领域的技术人员熟知在全长靶基因背景下鉴定最合适的靶核苷酸序列的方法。例如，可以合成和测试靶向靶基因的不同区域的多种dsRNA。可替代地，用如RNAseH等酶消化RNA转录物可以被用来确定RNA上构象对基因沉默敏感的位点。靶位点也可以使用计算机模拟(insilico)方法，例如使用被设计成基于靶向全长基因内的不同位点来预测基因沉默效力的计算机算法进行鉴定。
优选地，通过GAP(Needleman和Wunsch,1970)分析(GCG程序)，使用默认设置确定多核糖核苷酸的％同一性，其中查询序列是至少约21至约23个核苷酸长度，并且GAP分析经至少约21个核苷酸的区域比对这两个序列。在另一个实施例中，查询序列至少150个核苷酸长度，并且GAP分析经至少150个核苷酸的区域比对这两个序列。在一个另外的实施例中，查询序列至少300个核苷酸长度并且GAP分析经至少300个核苷酸的区域比对这两个序列。在又另一个实施例中，查询序列对应于全长靶RNA(例如mRNA)，并且GAP分析经全长靶RNA比对这两个序列。
便利地，该dsRNA可以在重组宿主细胞中产生自单个开放阅读框，其中正义和反义序列的侧翼是一个不相关序列，该不相关序列使得正义和反义序列可以杂交以与该不相关序列形成该dsRNA分子，从而形成环结构。可替代地，正义链和反义链可以在不具有开放阅读框的情况下产生，以保证在转基因宿主细胞中将不制造蛋白质。这两条链还可以被分别表达为两种转录物，一种编码正义链并且一种编码反义链。
可以在细胞内部或外部启动RNA双链体形成。该dsRNA可以是部分或完全双链的。RNA可以在体外或在体内酶促地或化学地合成。
该dsRNA相对于初级转录产物或完全加工的RNA不需要是全长的。在本领域熟知的是，长度为约19-23bp的小dsRNA可用于触发靶基因的基因沉默。通常，可以使用较高的同一性来补偿较短的序列的使用。此外，该dsRNA还可以包含单链区域，例如该dsRNA可以是部分或完全双链的。该dsRNA的双链区域可以具有至少约19至约23个碱基对的长度，任选是约19至约50个碱基对的序列，任选是约50至约100个碱基对的序列，任选是约100至约200个碱基对的序列，任选是约200至约500个碱基对的序列，并且任选是约500至约1000个或更多个碱基对的序列，直到对于其全长是双链的分子，大小对应于全长靶RNA分子。Bolognesi等人(2012，PLOS One[公共科学图书馆·综合],7(10):e47534,通过引用并入本文)教导了在具有南方玉米根虫(SCR；黄瓜十一星叶甲食根亚种)的人工饲料生物测定中生物活性需要大于或等于约60bp的dsRNA。
Mao等人(2007,Nature Biotechnology[自然生物技术],35(11):1307-1313)教导了表达针对昆虫有害生物(棉螟蛉，棉铃虫)的靶基因(CYP6AE14)的dsRNA构建体的转基因植物。摄食转基因植物的昆虫在其中肠中具有约19-23bp大小的靶基因的小RNA，相应地减少了CYP6AE14转录物和蛋白质。这表明了小RNA在减少昆虫有害生物中靶基因的表达方面是有效的。因此，约19bp、约20bp、约21bp、约22bp、约23bp、约24bp、约25bp、约26bp、约27bp、约28bp、约29bp、或约30bp的小RNA可有效减少昆虫有害生物中靶基因的表达。
可替代地，dsRNA可以包含具有至少19个碱基对的靶dsRNA，并且靶dsRNA可以在dsRNA“载体”或“填充物”序列内。例如，Bolognesi等人(2012)显示包含靶dsRNA的240bpdsRNA(其包含与靶序列具有100％同一性的21bp连续序列)在南方玉米根虫的生物测定中具有生物活性。本申请例示了与西方玉米根虫的生物测定中类似方法。该靶dsRNA可以具有至少19至约25个碱基对的长度，任选是约19至约50个碱基对的序列，任选是约50至约100个碱基对的序列，任选是约100至约200个碱基对的序列，任选是约200至约500个碱基对的序列，以及任选是约500至约1000或更多个碱基对的序列。与载体dsRNA序列组合，靶序列的dsRNA和载体dsRNA可具有至少约50至约100个碱基对的总长度，任选地约100至约200个碱基对的序列，任选地约200至约500个碱基对的序列，和任选地约500至约1000个或更多个碱基对的序列。
该dsRNA可以包含已知的核苷酸类似物或修饰的主链残基或连接物，其是合成的、天然存在的及非天然存在的。此类类似物的实例包括但不限于，硫代磷酸酯、氨基磷酸酯、甲基磷酸酯、手性甲基磷酸酯以及2-O-甲基核糖核苷酸。
如在此使用的，术语“特异性减少靶RNA的水平和/或由该RNA编码的靶标蛋白的产生”及其变体是指该dsRNA的一条链的一部分与该靶RNA足够同一，这样使得在细胞中存在的该dsRNA减少稳态水平和/或所述RNA的产生。在许多情况下，该靶RNA将是mRNA，并且在产生该mRNA的细胞中存在该dsRNA将导致所述蛋白的产生的减少。优选地，当与野生型细胞相比时，这一积累或产生被减少至少10％，更优选至少50％，甚至更优选至少75％，仍甚至更优选至少95％并且最优选100％。
抑制的后果可以通过检查该细胞或生物体的外表特性或通过生化技术加以确认，这些生化技术是例如但不限于，Northern杂交、逆转录、用微阵列监测基因表达、抗体结合、酶联免疫吸附测定(ELISA)、蛋白质印迹法、放射免疫测定(RIA)以及其他免疫测定。
本发明的干扰RNA可以包含一种dsRNA或多种dsRNA，其中每种dsRNA包含与在靶基因中的靶核苷酸序列至少部分互补的核苷酸序列或由其组成，并且在被昆虫有害生物摄入后起到下调所述靶基因的表达的作用。通过引用结合在此的WO 2006/046148中描述了这种类型的多联体RNA构建体。在本发明的上下文中，术语‘多个’意指至少两个，至少三个，至少四个等并且直到至少10、15、20个或至少30个。在一个实施例中，该干扰RNA包含单个dsRNA的多个拷贝，即结合至特定靶基因内的特定靶核苷酸序列的dsRNA的重复。在另一个实施例中，干扰RNA内的dsRNA包含与不同的靶核苷酸序列互补的不同核苷酸序列或由其组成。应该清楚的是，与结合于不同靶核苷酸序列的dsRNA组合的相同dsRNA的多个拷贝的组合是在本发明的范围内。
这些dsRNA可以安排为干扰RNA的一个连续区域或者可以通过接头序列的存在加以隔开。接头序列可以包含不与任何靶核苷酸序列或靶基因互补的短的随机核苷酸序列。在一个实施例中，该接头是条件性自切割RNA序列，优选是pH敏感性接头或疏水敏感性接头。在一个实施例中，该接头包含与内含子序列等效的核苷酸序列。本发明的接头序列的长度可以在从约1个碱基对到约10000个碱基对的范围内，其条件是该接头不会削弱干扰RNA下调靶基因的表达的能力。
除了这样的一种或多种dsRNA和任何接头序列，本发明的干扰RNA可以包含至少一种另外的多核苷酸序列。在本发明的不同实施例中，该另外的序列选自(i)能够保护干扰RNA免于RNA加工的序列，(ii)影响干扰RNA的稳定性的序列，(iii)允许蛋白质结合以例如促进干扰RNA被昆虫有害生物物种的细胞摄入的序列，(iv)促进大规模产生干扰RNA的序列，(v)作为结合于受体或结合于昆虫有害生物细胞表面上的分子以促进摄入的适体的序列，或(v)催化昆虫有害生物细胞中干扰RNA的加工并由此增强干扰RNA的效力的序列。用于增强RNA分子的稳定性的结构在本领域中是众所周知的并且进一步描述在WO 2006/046148中，该案通过引用结合在此。
该干扰RNA可以包含DNA碱基、非天然碱基或者糖-磷酸骨架的非天然骨架连接或修饰，例如以增强储存期间的稳定性或增强对核酸酶降解的抗性。此外，该干扰RNA可以由本领域的普通技术人员通过手动或自动反应化学地或酶促地产生。可替代地，该干扰RNA可以由编码其的多核苷酸转录。因此，在此提供了编码本发明的干扰RNA中的任一个的分离的多核苷酸。
微小RNA(miRNA)是不编码蛋白质的RNA，通常在约18至约25个核苷酸长度之间(植物中常见地是约20-24个核苷酸长度)。这些miRNA指导靶转录物的反式切割，负向调节参与各种调节及发育途径的基因的表达(Bartel,Cell[细胞],116:281-297(2004)；Zhang等人Dev.Biol.[发育生物学]289:3-16(2006))。照此，已经显示miRNA参与植物生长和发育的不同方面以及参与信号转导和蛋白质降解。此外，内源性小mRNA(包括miRNA)也可以参与生物胁迫反应，例如病原体攻击。自第一个miRNA在植物中发现以来(Reinhart等人Genes Dev[基因与发育]16:1616-1626(2002)，Park等人Curr.Biol[当代生物学]12:1484-1495(2002))，已经鉴定了数百种miRNA。另外，已经示出许多植物miRNA是跨非常趋异性分类单元而高度保守的。(Floyd等人Nature[自然]428:485-486(2004)；Zhang等人PlantJ[植物杂志]46:243-259(2006))。许多微RNA基因(MIR基因)已经被鉴定并且是在数据库(“miRBase”，通过万维网可获得)中公开可获得的。美国专利公开2005/0120415和2005/144669A1中也描述了miRNA，该专利的全部内容通过引用结合在此。
编码miRNA的基因产生长度70bp至300bp的初级miRNA(称作“pri-miRNA”)，其可以形成不完美的茎环结构。单个初级miRNA可以含有从1个至若干个miRNA前体。在动物中，初级miRNA在细胞核中由RNA酶III Drosha及其辅因子DGCR8/Pasha加工成约65nt的更短发夹RNA(前miRNA)。这种前miRNA随后输出至细胞质，在那里，它由另一种RNA酶III—切丁酶(Dicer)进一步加工，释放出大小约22nt的miRNA/miRNA*双链体。与动物相反，在植物中，初级miRNA加工为成熟miRNA完全在细胞核内利用单一RNA酶III—DCL1(切丁酶样1)进行。(Zhu Proc.Natl.Acad.Sci.[美国国家科学院院刊]105:9851-9852(2008))。关于微小RNA生物起源和功能的许多综述是可获得的，例如，参见，Bartel Cell[细胞]116:281-297(2004)；Murchison等人Curr.Opin.Cell Biol[细胞生物学新见]16:223-229(2004)；Dugas等人Curr.Opin.Plant Biol.[植物生物学新见]7:512-520(2004)和Kim NatureRev.Mol.Cell Biol[自然综述分子细胞生物学]6:376-385(2005)。
如在此使用的，术语“植物微小RNA前体分子”描述一种(约70nt-300nt)小非编码性RNA序列，它由植物酶加工以产生约19-24个核苷酸的产物，称作成熟微小RNA序列。这些成熟序列通过与信使RNA(mRNA)的互补性而具有调节作用。术语“人工植物微小RNA前体分子”描述在加工之前的非编码miRNA前体序列，其中经由以下方式使用该前体序列作为递送siRNA分子的主链序列，将这种miRNA前体分子的内源性天然miRNA/miRNA*双链体置换为一种非天然异源性miRNA的双链体(amiRNA/amiRNA*；例如siRNA/siRNA*)，后一种双链体然后与该siRNA序列一起加工为成熟miRNA序列。
在本发明的背景下，用于描述本发明的dsRNA的术语“有毒的”意指本发明的这些dsRNA分子以及此类dsRNA分子的组合作为对昆虫具有负面作用的口服有效昆虫控制试剂而起作用。当本发明的一种组合物被递送至昆虫时，这种结果典型地是该昆虫的死亡，或者该昆虫不以使该组合物可供该昆虫使用的来源为食。这样一种组合物可以是一种表达本发明的dsRNA的转基因植物。
“控制(control或controlling)”昆虫意指通过一种毒性作用抑制一种或多种昆虫有害生物存活、生长、摄食和/或繁殖的能力，或限制昆虫相关的损害或作物植物损失。“控制”昆虫可以是或可以不是意为杀死昆虫，尽管它优选意指杀死昆虫。控制靶昆虫的组合物具有针对靶昆虫的杀昆虫活性。
“递送(deliver或delivering)”一种组合物或dsRNA意指该组合物或dsRNA与一种昆虫接触，产生一种毒性作用和对昆虫的控制。可以按照许多公认方式，例如，通过昆虫经口摄取、经由转基因植物表达、一种或多种配制的组合物、一种或多种可喷洒的组合物、一种饵基(bait matrix)、或任何其他的领域公认的毒物递送系统来递送该组合物或dsRNA。
如在此使用的术语“昆虫”包括现在已知或以后鉴定的任何生物，其被分类在动物界、节肢动物门、昆虫纲中，包括但不限于鞘翅目(甲虫)、鳞翅目(蛾、蝴蝶)、双翅目(蝇)、原尾目、弹尾目(跳虫)、双尾目、石蛹目(跳蛀虫)、缨翅目(蛀虫、蠹虫)、蜉蝣目(蜉蝣)、蜻蜓目(蜻蜓、豆娘)、直翅目(草蜢、蟋蟀、蝈蝈)、竹节虫目(竹节虫)、蛩蠊目(岩石爬虫)、螳目、革翅目(地蜈蚣)、襀翅目(石蝇)、纺足目(纺足虫)、缺翅目、等翅目(白蚁)、螳螂目(螳螂)、蜚蠊目(蟑螂)、半翅目(蝽虫、蝉、叶蝉、蚜虫、蚧)、缨翅目(牧草虫)、啮虫目(书虱和树虱)、虱毛目(虱；包括但不限于钝角亚目、丝角亚目和虱亚目)、脉翅目(草蜻蛉、蝶角蛉、螳蛉、蚁蛉)、膜翅目(蜜蜂、蚁、黄蜂)、毛翅目(毛翅蝇)、蚤目(跳蚤)、长翅目(蝎蛉)、捻翅目(捻翅寄生虫)的昆虫及其任何组合。
如在此使用的，“鞘翅目昆虫”是指鞘翅目的任何成员，包括鞘翅目植物有害生物。鞘翅目昆虫包括但不限于现在已知或以后鉴定的任何鞘翅目昆虫，包括原鞘亚目、粘食亚目、肉食亚目和多食亚目的那些及其任何组合。
“根萤叶甲属”是一种甲虫属(来自鞘翅目)，通常称为“玉米根虫”或“黄瓜甲虫”。根萤叶甲属昆虫是作物植物的有害生物，这些昆虫包括但不限于，巴氏根萤叶甲(北方玉米根虫；NCR)、西方玉米根萤叶甲(西方玉米根虫；WCR)、黄瓜十一星叶甲食根亚种(南方玉米根虫；SCR)、墨西哥玉米根萤叶甲(墨西哥玉米根虫；MCR)以及南美叶甲。在本发明的背景下，术语“玉米根虫”或“黄瓜甲虫”与术语“根萤叶甲属”是可互换的。
根据本发明的鞘翅目昆虫有害生物的其他非限制性实例包括马铃薯叶甲属，如马铃薯叶甲(科罗拉多马铃薯甲虫)；叶甲属，如黑杨叶甲(黑杨叶甲虫)；咪小蠹属，如咖啡果小蠹(H.hampei或coffee berry borer)；象虫属，如玉米象(S.zeamais或maize weevil)；毛跳甲属，如烟草跳甲(E.hirtipennis或tobacco flea beetle)和马铃薯跳甲(E.cucumeris或potato flea beetle)；条跳甲属，如十字花科植物跳甲(P.cruciferae或crucifer flea beetle)和西方黑色跳甲(P.pusilla或western black flea beetle)；花象属，如胡椒茎象甲(A.eugenii或pepper weevil)；金针虫属，如金针虫(H.memnonius或wireworm)；纹叩甲属，如普通梳爪叩头甲(金针虫)；荚象甲属，如甘蓝荚象甲(C.assimilis或cabbage seedpod weevil)；条跳甲属，如十字花科条跳甲(P.cruciferae或cruciferflea beetle)；Aeolus属，如A.mellillus(金针虫)；Aeolus属，如A.mancus(小麦铁线虫)；砂铁线属，如砂铁线虫(H.uhlerii或sand wireworm)；尖隐喙象属，如玉米隐啄象(玉米谷象)、S.zeae(梯牧草谷象)、牧草长喙象(早熟禾谷象)、和南方玉米谷象(S.callosus或southern corn billbug)；鳃角金龟属(蛴螬)；凹胫跳甲属，如玉米铜色跳甲(玉米跳甲)；弧丽金龟属，如日本甲虫(P.japonica或Japanese beetle)；食植瓢虫属，如墨西哥豆瓢虫(E.varivestis或Mexican bean beetle)；萤叶甲属，如C.trifurcate(豆叶甲)；豆芫菁属，如E.Pestifera和E.lemniscata(斑蝥)；以及前述的任何组合。
“根萤叶甲属生活期”或“玉米根虫生活期”意指根萤叶甲属物种的卵、幼虫、蛹或成虫发育形式。
“有效的昆虫控制量”意指dsRNA的浓度，它通过一种毒性作用抑制昆虫存活、生长、摄食和/或繁殖的能力，或限制昆虫相关的损害或作物植物损失。“有效的昆虫控制量”可以或可以不意指杀死昆虫的浓度，尽管优选它意指杀死昆虫。
术语“农用化学活性成分”是指化学和/或生物学组合物，如在此描述的那些，它们在杀害、预防或者控制所不希望的有害生物的生长方面有效，如植物、昆虫、小鼠、微生物、藻类、真菌、细菌以及类似物(如杀生物活性成分)。本发明的干扰RNA分子是农用化学活性成分。
“农业上可接受的载体”包括有益于活性成分(如本发明的干扰RNA分子)施用的佐剂、混合剂、增强剂等。合适的载体不应对有价值的作物具有植物毒性，特别是在作物存在下施用组合物时所用的浓度下，并且不应与在此的活性成分的化合物(即本发明的干扰RNA或其他组合物成分)发生化学反应。此类混合物可以设计用于直接施用于作物，或者可以是浓缩物或配制品，其通常在施用前用另外的载体和佐剂稀释。它们可包括惰性或活性组分，并且可以是固体，如例如尘剂、颗粒剂、水可分散颗粒剂、或可湿性粉剂；或液体，如例如可乳化的浓缩物、溶液、乳液或悬浮液。合适的农业载体可包括液体载体，例如水、甲苯、二甲苯、石脑油、作物油、丙酮、甲基乙基酮、环己酮、三氯乙烯、全氯乙烯、乙酸乙酯、乙酸戊酯、乙酸丁酯、丙二醇单甲醚和二乙二醇单甲醚、甲醇、乙醇、异丙醇、戊醇、乙二醇、丙二醇、甘油以及类似物。水通常是用以稀释浓缩物的选用载体。合适的固体载体可包括滑石、叶腊石粘土、硅石、凹凸棒粘土、硅藻土(kieselguhr)、白垩、硅藻土(diatomaceous earth)、石灰、碳酸钙、膨润土、漂白土、棉子壳、小麦粉、大豆粉、浮石、木粉、核桃壳粉、木质素以及类似物。
对于本发明，农业上可接受的载体还可以包括携带昆虫控制试剂(即本发明的干扰RNA分子)的非致病性减毒微生物菌株。在这种情况下，携带干扰RNA的微生物也可称为昆虫控制试剂。可将微生物工程化以表达靶基因的核苷酸序列以产生干扰RNA分子，该干扰RNA分子包含与典型地在昆虫细胞内发现的RNA序列同源或互补的RNA序列。昆虫暴露于微生物导致微生物的摄入和由干扰RNA分子或其片段或衍生物直接或间接介导的靶基因表达的下调。
在另一个实施例中，干扰RNA分子可以被包封在合成基体(如聚合物)中，并施用于宿主(如植物)的表面。昆虫摄入宿主细胞允许将昆虫控制试剂递送至昆虫并导致宿主中靶基因的下调。
本发明的组合物，例如包含本发明的干扰RNA分子和农业上可接受的载体的组合物，可用于常规农业方法中。例如，可以将本发明的组合物与水和/或肥料混合，并且能以任何装置将其在出苗前和/或出苗后施用至所希望的场所，该装置是如飞机喷雾桶、灌溉设备、直接注入喷雾设备、背囊喷雾桶、家畜浸渍槽、在地面喷雾中使用的农场设备(例如，喷管式喷雾器、手动喷雾器)，以及类似工具。所希望的场所可以是土壤、植物以及类似的场所。
本发明的组合物可以在种子收获和种子播种之间的任何时间；播种期间或之后；和/或发芽后以任何生理状态施用于种子或植物繁殖体。优选地是，种子或植物繁殖体处于足够耐用的状态，在处理过程中不会造成或造成最小的损害，包括物理损害或生物损害。配制品可以使用常规的涂覆技术以及机器，如流化床技术、滚筒研磨方法、静态转动(rotostatic)种子处理器和转鼓包衣器施用到种子或植物繁殖体上。
如在此使用的“表达盒”意指能够在适当的宿主细胞中指导特定的核酸序列的表达的核酸序列，包含如下启动子，该启动子可操作地连接至感兴趣的核酸序列上，该核酸序列可操作地连接至终止信号序列。它还典型地包含正确翻译该核酸序列所需要的序列。包含该感兴趣的核酸序列的表达盒可以是嵌合的，意味着至少一个它的组分相对于至少一个它的其他组分是异源的。该表达盒还可以是一种天然存在的表达盒，但已经是以对于异源表达有用的重组形式而获得的。然而，典型地，该表达盒相对于该宿主而言是异源的，即该表达盒的特定核酸序列不是天然存在于该宿主细胞中的，并且必须已经通过转化事件引入到该宿主细胞或该宿主细胞的祖先中。该核酸序列在该表达盒中的表达可以是在例如组成型启动子或诱导型启动子的控制之下，只有当该宿主细胞暴露于某一特定的外部刺激时该启动子才引发转录。在多细胞生物体(如植物)的情况下，该启动子也可以特异于特定组织、或器官、或者发育阶段。
“基因”是限定的区域，该区域位于基因组之内，并且除了前述的编码序列之外，它还包含其他负责该编码部分的表达控制(也就是转录和翻译)的主要调节核酸序列。基因还可以包含其他5'和3'未翻译序列和终止序列。其他可能存在的元件是，例如，内含子。
如在此使用的，术语“种植者”意指从事农业，养殖生物机体(如玉米植物，例如玉米)用于食物、饲料、或原料的个人或实体。
“异源”核酸序列是天然地不与将该核酸序列引入其中的宿主细胞相关联的核酸序列，包括天然存在的核酸序列的非天然存在的多拷贝。
“同源”核酸序列是与其被引入的宿主细胞天然相关的核酸序列。
“杀昆虫”被定义为有毒的生物活性，它能够控制昆虫，优选地通过杀死它们来控制。
本发明的“分离的”核酸分子或核苷酸序列或核酸构建体或dsRNA分子或蛋白通常与其天然环境分离而存在并且因此不是自然的产物。分离的核酸分子或核苷酸序列或核酸构建体或dsRNA分子或蛋白能以纯化形式存在，或能存在于非天然环境(例如像重组宿主或宿主细胞，如转基因植物或转基因植物细胞)中。
在本发明的背景下，后缀“mer”前的数字指示亚基的特定数目。当应用于RNA或DNA时，这指定了分子中碱基的数目。例如，具有序列ACUGGUCGCGUUGCAUGCU的mRNA的19个核苷酸子序列是“19-mer”。
“植物”是在发育的任何阶段的任何植物，特别是种子植物。
“植物细胞”是植物的结构和生理单位，包含原生质体和细胞壁。植物细胞可以处于分离的单细胞或培养细胞的形式，或者是作为较高级的组织单位(例如像，植物组织、植物器官、或整株植物)的一部分。
“植物细胞培养物”意指植物单元(例如像，原生质体、细胞培养物细胞、植物组织中的细胞、花粉、花粉管、胚珠、胚囊、接合子以及处于不同发育阶段的胚)的培养物。
植物材料：是指叶、茎、根、花或花的部分、果实、花粉、卵细胞、接合子、种子、切条、细胞或组织培养物、或一种植物的任何其他部分或产物。
“植物器官”是植物的独特而明显的已结构化并且分化的部分，如根、茎、叶、花蕾或胚。
如在此使用的“植物组织”意指组织化成结构和功能单元的一组植物细胞。包括植物中或培养物中的任何植物组织。这个术语包括但不限于全株植物、植物器官、植物种子、组织培养物以及被组织化成结构和/或功能单元的任何植物细胞群组。这个术语与如以上列出的或由该定义以其他方式涵盖的任何具体类型的植物组织的联合应用或单独应用并不旨在排除任何其他类型的植物组织。
玉米根虫“转录组”是玉米根虫细胞中的所有或几乎所有的核糖核酸(RNA)转录物的集合。
“转化”是用于将异源核酸引入到宿主细胞或生物的方法。具体地，“转化”意指DNA分子稳定地整合到感兴趣生物的基因组中。
“转化的/转基因的/重组的”是指异源核酸分子已经引入其中的宿主生物如细菌或植物。该核酸分子可以被稳定地整合到宿主的基因组中，或者该核酸分子还可以作为染色体外分子存在。这样的染色体外分子能够自主复制。转化的细胞、组织或植物应当理解为不仅涵盖转化过程的终产物，而且涵盖其转基因子代。“非转化的”、“非转基因的”、或“非重组的”宿主是指不含有该异源的核酸分子的野生型生物，例如细菌或植物。
在此使用的对于DNA或RNA碱基和氨基酸给出的命名法如在37C.F.R.§1.822中给出。
本发明是基于以下出乎意料的发现，设计用于靶向可从在此描述的根萤叶甲属基因转录的mRNA的双链RNA(dsRNA)或小干扰RNA(siRNA)，对根萤叶甲属昆虫有害生物具有毒性并且可以用于控制植物的根萤叶甲属或鞘翅目侵染并赋予转基因植物对根萤叶甲属或鞘翅目侵染的耐受性。因此，在一个实施例中，本发明提供了包含正义链和反义链的双链RNA(dsRNA)分子，其中该反义链的核苷酸序列与可从本披露描述的根萤叶甲属昆虫基因转录的mRNA多核苷酸的一部分互补，其中该dsRNA分子对根萤叶甲属昆虫或鞘翅目植物有害生物是具有毒性的。
本领域已知，与靶序列不完全互补的dsRNA分子(例如，与靶基因仅具有95％同一性)对控制鞘翅目有害生物是有效的(参见，例如，Narva等人，美国专利号9,012,722)。本发明提供了包含至少一种dsRNA的干扰RNA分子，其中该dsRNA是包含退火的至少部分互补的链的双链RNA的区域。该dsRNA的一条链包含具有至少19、至少20、至少21、至少22、至少23、至少24、至少25、至少26、至少27、至少28、至少29、至少30、至少35、至少40、至少45、至少50、至少55、至少60、至少65、至少70、至少75、至少80、至少85、至少90、至少95、至少100、至少110、至少120、至少130、至少140、至少150、至少160、至少170、至少180、至少190、至少200、至少210、至少220、至少230、至少240、至少250、至少260、至少270、至少280、至少290或至少300个连续核苷酸的序列，其与根萤叶甲属靶基因内的靶核苷酸序列是至少部分互补的。该干扰RNA分子(i)至少与SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列具有至少80％同一性、至少85％同一性、至少86％同一性、至少87％同一性、至少88％同一性、至少89％同一性、至少90％同一性、至少91％同一性、至少92％同一性、至少93％同一性、至少94％同一性、至少95％同一性、至少96％同一性、至少97％同一性、至少98％同一性、至少99％同一性、或100％同一性；(ii)至少包含SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列；(iii)至少包含编码由SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400编码的氨基酸序列的核苷酸序列的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列，或者(iv)可以在严格条件下与选自下组的多核苷酸杂交，该组由以下组成：SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400，及其互补序列，其中该干扰RNA分子对鞘翅目植物有害生物具有杀昆虫活性。
在一些实施例中，该干扰RNA分子包含至少两种dsRNA，其中每种dsRNA包含核苷酸的序列，该序列与该靶基因内的靶核苷酸序列是至少部分互补的。在一些实施例中，每种dsRNA包含不同的核苷酸序列，该核苷酸序列与该靶基因内的不同靶核苷酸序列是互补的。在其他实施例中，每种dsRNA包含不同的核苷酸序列，该核苷酸序列与两个不同的靶基因内的靶核苷酸序列是互补的。
在一些实施例中，该干扰RNA分子包含如下dsRNA，该dsRNA可以包含从至少18至约25个连续核苷酸(例如18、19、20、21、22、23、24、25、26、27、28或29)到至少约300个连续核苷酸，基本由其组成或由其组成。可以在3'末端、5'末端或3'和5'末端二者处添加另外的核苷酸，以促进RNA分子的操作但是不实质地影响该dsRNA分子在RNA干扰(RNAi)中的基本特征或功能。
在一些实施例中，该干扰RNA分子包含如下dsRNA，该dsRNA包含与SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的至少19、至少20、至少21、至少22、至少23、至少24、至少25、至少26、至少27、至少28、至少29、至少30、至少35、至少40、至少45、至少50、至少55、至少60、至少65、至少70、至少75、至少80、至少85、至少90、至少95、至少100、至少110、至少120、至少130、至少140、至少150、至少160、至少170、至少180、至少190、至少200、至少210、至少220、至少230、至少240、至少250、至少260、至少270、至少280、至少290、或至少300个连续核苷酸，或其互补序列互补的反义链。在其他实施例中，dsRNA的该部分包含SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的至少从19、20或21个连续核苷酸到至少19、至少20、至少21、至少22、至少23、至少24、至少25、至少26、至少27、至少28、至少29、至少30、至少35、至少40、至少45、至少50、至少55、至少60、至少65、至少70、至少75、至少80、至少85、至少90、至少95、至少100、至少110、至少120、至少130、至少140、至少150、至少160、至少170、至少180、至少190、至少200、至少210、至少220、至少230、至少240、至少250、至少260、至少270、至少280、至少290、或至少300个连续核苷酸，或其互补序列，基本由其组成或由其组成。
在其他实施例中，本发明的干扰RNA分子包含如下dsRNA，该dsRNA包含由N至N+20个核苷酸组成的SEQ ID NO:181-210的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成。例如，本发明的干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:181的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:181的核苷酸1至核苷酸776。换言之，被靶向的mRNA的该部分包含776个SEQ ID NO:181的21个连续核苷酸(即21-mers)的子序列中的任一个，或其任何互补序列。将认识到的是，这些776个21个连续核苷酸的子序列包括来自SEQ ID NO:121和SEQ ID NO:151的所有可能的21个连续核苷酸的子序列，以及它们的互补序列，因为SEQ ID NO 121、151和181都针对相同的靶标，即BPA_15366。类似地将认识到的是，SEQ ID NO:181-210的所有21-mer子序列及其所有互补序列子序列包括SEQ ID NO:121-180的所有可能的21个连续核苷酸的子序列，及其互补序列子序列。
类似地，本发明的干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:182的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:182的核苷酸1至核苷酸771。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ IDNO:183的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQID NO:183的核苷酸1至核苷酸2907。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:184的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:184的核苷酸1至核苷酸1600。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:185的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:185的核苷酸1至核苷酸2410。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:186的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:186的核苷酸1至核苷酸2802。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:187的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:187的核苷酸1至核苷酸3681。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:188的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:188的核苷酸1至核苷酸651。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:189的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ IDNO:189的核苷酸1至核苷酸673。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:190的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:190的核苷酸1至核苷酸2664。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:191的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:191的核苷酸1至核苷酸438。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:192的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:192的核苷酸1至核苷酸2458。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:193的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:193的核苷酸1至核苷酸3254。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:194的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:194的核苷酸1至核苷酸3632。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:195的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:195的核苷酸1至核苷酸7611。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:196的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ IDNO:196的核苷酸1至核苷酸1008。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:197的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:197的核苷酸1至核苷酸2992。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:198的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:198的核苷酸1至核苷酸1192。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:199的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:199的核苷酸1至核苷酸7626。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:200的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:200的核苷酸1至核苷酸2580。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:201的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:201的核苷酸1至核苷酸4628。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:202的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ IDNO:202的核苷酸1至核苷酸1557。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:203的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:203的核苷酸1至核苷酸1019。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:204的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:204的核苷酸1至核苷酸677。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:205的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:205的核苷酸1至核苷酸764。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:206的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:206的核苷酸1至核苷酸1830。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:207的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:207的核苷酸1至核苷酸3225。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:208的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:208的核苷酸1至核苷酸1003。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQID NO:209的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:209的核苷酸1至核苷酸1419。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:210的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:210的核苷酸1至核苷酸5206。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:374的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:374的核苷酸1至核苷酸7112。
在仍其他实施例中，本发明的干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ IDNO:121-210，SEQ ID NO:274-276，SEQ IDNO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400，或其互补序列，基本上由其组成或由其组成。
在本发明的干扰RNA分子的其他实施例中，本发明的dsRNA的反义链的核苷酸序列包含SEQ ID NO:211-240或SEQ ID NO:375的核苷酸序列，基本由其组成或由其组成。本发明的dsRNA的反义链的核苷酸序列在3’或5’末端可以缺失一个核苷酸或在3’末端、5’末端或两者处可以添加多达6个核苷酸(以任何组合)，以实现反义链基本由SEQ ID NO:211-240或SEQ ID NO:375的任何19-mer、任何20-mer、或任何21-mer核苷酸序列组成，因为将理解的是，缺失1个核苷酸或添加多达6个核苷酸不实质地影响本发明的双链RNA分子的基本特征或功能。这类另外的核苷酸可以是沿靶序列延伸反义链互补性的核苷酸和/或这类核苷酸可以是促进RNA分子或编码该RNA分子的核酸分子的操作的核苷酸，如本领域的普通技术人员将已知。例如，在3'末端可以存在TT突出端，使用该突出端来稳定siRNA双链体并且不影响该siRNA的特异性。
在本发明的一些实施例中，该干扰RNA分子的双链RNA的反义链可以与该靶RNA多核苷酸完全互补，或该反义链可以与靶RNA多核苷酸基本上互补或部分互补。该干扰RNA分子的dsRNA可以包含如下dsRNA，该dsRNA是包含基本上互补的退火链的双链RNA的区域，或是包含完全互补的退火链的双链RNA的区域。基本上或部分互补意指反义链和靶RNA多核苷酸可以按约1、2、3、4、5、6、7、8、9或10个核苷酸配对错配。可以将这类错配引入反义链序列中，例如，靠近3'末端，以便增强双链RNA分子由切丁酶加工，以便在插入本发明的嵌合核酸分子或人工微小RNA前体分子内的siRNA分子中重复错配样式等，如本领域的普通技术人员将已知。这类修饰将在双链体的一个末端削弱碱基配对作用并且产生链非对称性，因此增大反义链(而非正义链)受到加工和沉默预期基因的几率(Geng和Ding“Double-mismatchedsiRNAs enhance selective gene silencing of a mutant ALS-causing Allele1[双错配siRNA增强造成突变ALS的等位基因1的选择性基因沉默]”Acta Pharmacol.Sin.[中国药理学报]29:211-216(2008)；Schwarz等人“Asymmetry in the assembly of the RNAienzyme complex[RNAi酶复合体的组合体中的非对称性]”Cell[细胞]115:199-208(2003))。
在本发明的一些实施例中，该干扰RNA包含一种含有短发夹RNA(shRNA)分子的dsRNA。shRNA在细胞中的表达典型地通过将质粒或重组载体递送例如进入转基因植物(如转基因玉米)中而完成。
本发明涵盖一种包含本发明的干扰RNA的核酸构建体。本发明进一步涵盖如下核酸分子，该核酸分子编码本发明的至少一种干扰分子。本发明进一步涵盖如下核酸构建体，该核酸构建体包含本发明的至少一种干扰分子，或包含编码本发明的至少一种干扰分子的核酸分子。本发明进一步涵盖如下核酸构建体，其中该核酸构建体是表达载体。本发明进一步涵盖如下重组载体，该重组载体包含可操作地连接至编码本发明的干扰RNA分子的核苷酸序列的调节序列。调节序列可以意指一种启动子、增强子、转录因子结合位点、隔离子、沉默子、或参与基因表达的任何其他DNA元件。
本发明进一步涵盖如下嵌合核酸分子，该嵌合核酸分子包含具有dsRNA的反义链的干扰RNA分子，该dsRNA可操作地连接至植物微小RNA前体分子。在一些实施例中，该嵌合核酸分子包含如下反义链，该反义链具有SEQ ID NO:181-210的21-mer子序列中的任一个、或其任何互补序列的核苷酸序列，该反义链可操作地连接至植物微小RNA前体分子。在一些实施例中，该植物微小RNA前体分子是一种玉米微小RNA前体。
在一些实施例中，本发明涵盖一种人工植物微小RNA前体分子，该前体分子包含本发明的干扰RNA分子的dsRNA的一条反义链。在其他实施例中，该人工植物微小RNA前体分子包含具有SEQ ID NO:211-240的19-mer、20-mer或21-mer子序列中的任一个的核苷酸序列的反义链。使用人工植物微小RNA将感兴趣的核苷酸序列(例如人工miRNA；siRNA/siRNA*)递送进植物中在本领域是已知的(参见例如，Schwab等人2006The Plant Cell[植物细胞]18:1121-1133和在此的实例部分)。在本发明中，这些人工微小RNA是其中插入植物微小RNA前体主链和昆虫siRNA序列的嵌合或杂交分子。如本领域的普通技术人员将理解，典型地希望维持错配，这些错配正常情况下在植物微小RNA前体序列中存在于被置换入该植物微小RNA前体主链中的任何核苷酸序列中。在仍其他实施例中，该人工植物微小RNA前体包含玉米微小RNA前体分子的部分。任何玉米微小RNA(miRNA)前体都适于本发明的这些组合物和方法。非限制性实例包括miR156、miR159、miR160、miR162、miR164、miR166、miR167、miR168、miR169、miR171、miR172、miR319、miR390、miR393、miR394、miR395、miR396、miR397、miR398、miR399、miR408、miR482、miR528、miR529、miR827、miR1432以及现在已知或后来被鉴定出的任何其他植物miRNA前体。
在一些实施例中，本发明涵盖干扰RNA分子、核酸构建体、核酸分子、或重组载体，其包含本发明的干扰RNA分子的dsRNA的至少一条链，或包含本发明的嵌合核酸分子，或包含本发明的人工植物微小RNA。在一些实施例中，该核酸构建体包含本发明的核酸分子。在其他实施例中，该核酸构建体是一种重组表达载体。
在一些实施例中，本发明的干扰RNA分子对根萤叶甲属昆虫具有杀昆虫活性。在一些实施例中，该根萤叶甲属昆虫选自下组，该组由以下组成：巴氏根萤叶甲(北方玉米根虫)、西方玉米根萤叶甲(西方玉米根虫)、黄瓜十一星叶甲食根亚种(南方玉米根虫)、黄瓜条根萤叶甲(D.balteata)(带状黄瓜甲虫)、黄瓜十一星叶甲(D.undecimpunctataundecimpunctata)(西方斑点黄瓜甲虫)、斯格尼根萤叶甲(D.significata)(3斑叶甲)、南美叶甲(菊花甲虫)、墨西哥玉米根萤叶甲(墨西哥玉米根虫)、班尼根萤叶甲(D.beniensis)、克里斯塔根萤叶甲(D.cristata)、科威根萤叶甲(D.curvipustulata)、双斑根萤叶甲(D.dissimilis)、华丽根萤叶甲(D.elegantula)、伊墨根萤叶甲(D.emorsitans)、禾本科根萤叶甲(D.graminea)、伊斯帕尼根萤叶甲(D.hispanolae)、莱米妮根萤叶甲(D.lemniscata)、赭腿根萤叶甲(D.linsleyi)、米勒根萤叶甲(D.milleri)、钱币形根萤叶甲(D.nummularis)、扇叶根萤叶甲(D.occlusa)、普拉根萤叶甲(D.porracea)、蜗牛根萤叶甲(D.scutellata)、胫骨根萤叶甲(D.tibialis)、三线根萤叶甲(D.trifasciata)以及微绿根萤叶甲(D.viridula)。在另外的实施例中，该根萤叶甲属昆虫是西方玉米根萤叶甲(西方玉米根虫)、黄瓜十一星叶甲食根亚种(南方玉米根虫)、或巴氏根萤叶甲(北方玉米根虫)。在一些实施例中，该靶基因的编码序列包含选自下组的序列，该组包含SEQ ID NO:91-120和SEQ ID NO:372。
在一些实施例中，本发明涵盖包含一种或多种或者两种或更多种本发明的干扰RNA分子的组合物。在一些实施例中，干扰RNA分子存在于同一核酸构建体上、不同的核酸构建体上或其任何组合上。例如，本发明的一种干扰RNA分子可以存在于核酸构建体上，并且本发明的第二干扰RNA分子可以存在于同一核酸构建体上或单独的第二核酸构建体上。本发明的第二干扰RNA分子可以针对相同的靶基因或不同的靶基因。
在一些实施例中，本发明涵盖包含如下干扰RNA分子的组合物，该干扰RNA分子包含至少一种dsRNA，其中该dsRNA是包含退火的互补链的双链RNA的区域。该dsRNA的一条链包含具有至少19、至少20、至少21、至少22、至少23、至少24、至少25、至少26、至少27、至少28、至少29、至少30、至少35、至少40、至少45、至少50、至少55、至少60、至少65、至少70、至少75、至少80、至少85、至少90、至少95、至少100、至少110、至少120、至少130、至少140、至少150、至少160、至少170、至少180、至少190、至少200、至少210、至少220、至少230、至少240、至少250、至少260、至少270、至少280、至少290或至少300个连续核苷酸的序列，其与根萤叶甲属靶基因内的靶核苷酸序列是至少部分互补的。该干扰RNA分子(i)至少与SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列具有至少80％同一性、至少85％同一性、至少86％同一性、至少87％同一性、至少88％同一性、至少89％同一性、至少90％同一性、至少91％同一性、至少92％同一性、至少93％同一性、至少94％同一性、至少95％同一性、至少96％同一性、至少97％同一性、至少98％同一性、至少99％同一性、或100％同一性；(ii)至少包含SEQ ID NO:121-210，SEQ IDNO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列；(iii)至少包含编码由SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400编码的氨基酸序列的核苷酸序列的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列，或者(iv)可以在严格条件下与选自下组的多核苷酸杂交，该组由以下组成：SEQ ID NO:121-210，SEQ IDNO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400，及其互补序列。
在一些实施例中，本发明涵盖包含一种干扰RNA分子的组合物，该干扰RNA分子包含两种或更多种dsRNA，其中这两种或更多种dsRNA各自包含不同的反义链。在一些实施例中，本发明涵盖包含至少两种或更多种干扰RNA分子的组合物，其中这两种或更多种干扰RNA分子各自包含含有不同的反义链的dsRNA。这两种或更多种干扰RNA可以在相同的核酸构建体上、在不同的核酸构建体上、或其任何组合上存在。在其他实施例中，该组合物包含含有如下反义链的RNA分子，该反义链基本上由至少包含SEQ ID NO:211-240或SEQ ID NO:375的19个连续核苷酸的片段的核苷酸序列组成，并且在一些实施例中，该组合物可进一步包含含有如下反义链的RNA分子，该反义链基本上由至少包含SEQ ID NO:211-240或SEQ IDNO:375的19个连续核苷酸的片段的第二核苷酸序列组成；并且在一些实施例中，该组合物可进一步包含含有如下反义链的RNA分子，该反义链基本上由至少包含SEQ ID NO:211-240或SEQ ID NO:375的19个连续核苷酸的片段的第三核苷酸序列组成，并且在一些实施例中，该组合物可进一步包含含有如下反义链的RNA分子，该反义链基本上由至少包含SEQ IDNO:211-240或SEQ ID NO:375的19个连续核苷酸的片段的第四核苷酸序列组成，并且在一些实施例中，该组合物可进一步包含含有如下反义链的RNA分子，该反义链基本上由至少包含SEQ ID NO:211-240或SEQ ID NO:375的19个连续核苷酸的片段的第五核苷酸序列组成，并且在一些实施例中，该组合物可进一步包含含有如下反义链的RNA分子，该反义链基本上由至少包含SEQ ID NO:211-240或SEQ ID NO:375的19个连续核苷酸的片段的第六核苷酸序列组成，并且在一些实施例中，该组合物可进一步包含含有如下反义链的RNA分子，该反义链基本上由至少包含SEQ ID NO:211-240或SEQ ID NO:375的19个连续核苷酸的片段的第七核苷酸序列组成。在其他实施例中，该组合物可以包含两种或更多种核酸分子，其中这两种或更多种核酸分子各自编码不同的干扰RNA分子。在其他实施例中，该组合物可以包含两种或更多种核酸构建体，其中这两种或更多种核酸构建体各自包含编码不同干扰RNA的核酸分子。
在其他实施例中，该组合物包含本发明的两种或更多种核酸构建体、两种或更多种核酸分子、两种或更多种嵌合核酸分子、两种或更多种人工植物微小RNA前体，其中这两种或更多种核酸构建体、两种或更多种核酸分子、两种或更多种嵌合核酸分子、或两种或更多种人工植物微小RNA前体各自包含一条不同的反义链。
在一些实施例中，本发明涵盖用于抑制在此描述的根萤叶甲属昆虫基因表达的杀昆虫组合物，该组合物包含本发明的干扰RNA和农业上可接受的载体。在一些实施例中，该可接受的农用载体是一种表达本发明的干扰RNA的转基因生物体。在一些实施例中，该转基因生物体可以是一种表达本发明的干扰RNA的转基因植物，当被靶鞘翅目植物有害生物摄食时导致该靶鞘翅目植物有害生物停止摄食、生长、或繁殖，或导致该靶鞘翅目植物有害生物的死亡。在其他实施例中，该转基因植物是一种转基因玉米植物并且该靶有害生物是一种根萤叶甲属昆虫有害生物。在仍其他实施例中，该根萤叶甲属昆虫有害生物选自下组，该组由以下各项组成：巴氏根萤叶甲(北方玉米根虫)，西方玉米根萤叶甲(西方玉米根虫)，黄瓜十一星叶甲食根亚种(南方玉米根虫)，黄瓜条根萤叶甲(带状黄瓜甲虫)，黄瓜十一星叶甲(西方斑点黄瓜甲虫)，斯格尼根萤叶甲(3斑叶甲)，南美叶甲(菊花甲虫)，墨西哥玉米根萤叶甲(墨西哥玉米根虫)。
在其他实施例中，该转基因生物体选自(但不限于)下组，该组由以下组成：表达本发明的干扰RNA分子的酵母、真菌、藻类、细菌、病毒、或节肢动物。在一些实施例中，该转基因生物体是如下病毒，例如当侵染昆虫宿主时表达本发明的干扰RNA分子的昆虫杆状病毒。这样的一种杆状病毒比野生型未转化杆状病毒针对靶昆虫可能更具剧毒。在其他实施例中，该转基因生物体是施用至靶有害生物出现或已知已经出现的环境的转基因细菌。在一些实施例中，使用能够在植物组织内生活并复制的非致病共生细菌(所谓的内寄生菌)，或能够定居在叶际或根际的非致病共生细菌(所谓的附生菌)。此类细菌包括以下属的细菌：农杆菌属、产碱菌属、固氮螺菌属、定氮菌属、芽孢杆菌属、棒形杆菌属、肠杆菌属、欧文菌属、黄杆菌属、克雷白菌属、假单胞菌属、根瘤菌属、沙雷菌属、链霉菌属以及黄单胞菌属。共生真菌(如木霉属和胶枝霉属)也是为了相同目的表达本发明的干扰RNA分子的可能宿主。
在一些实施例中，可接受的农用载体是可用于将包含该干扰RNA分子的组合物施用至植物或种子的配制品。在一些实施例中，这些干扰RNA分子稳定对抗降解，由于其双链性质和DNA酶/RNA酶抑制剂的引入。例如，可以通过包括胸苷或尿苷核苷酸3'突出端来稳定dsRNA或siRNA。包含在本发明的组合物中的dsRNA或siRNA能大量地以工业规模化学地合成。可获得的方法将是通过化学合成或通过使用生物剂。
在其他实施例中，该配制品包含转染促进剂。在其他实施例中，该转染促进剂是含脂质化合物。在另外的实施例中，该含脂质化合物选自下组，该组由以下组成：Lipofectamine、Cellfectin、DMRIE-C、DOTAP以及Lipofectin。在另一个实施例中，该含脂质化合物是Tris阳离子脂质。
在一些实施例中，该配制品进一步包含核酸冷凝剂。该核酸冷凝剂可以是本领域已知的任何此类化合物。核酸冷凝剂的实例包括但不限于，亚精胺(N-[3-氨基丙基]-1,4-丁二胺)、鱼精蛋白硫酸盐、聚赖氨酸以及其他带正电的肽。在一些实施例中，该核酸冷凝剂是亚精胺或鱼精蛋白硫酸盐。
在仍另外的实施例中，该配制品进一步包含缓冲的蔗糖或磷酸盐缓冲生理盐水。
在一些实施例中，本发明涵盖转基因植物或其部分，其包含本发明的干扰RNA分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子、和/或组合物，其中当与对照植物相比时，该转基因植物对鞘翅目昆虫或根萤叶甲属昆虫具有增强的抗性。在其他实施例中，该转基因植物或其部分是转基因玉米植物或其部分。本发明进一步涵盖本发明的转基因植物的转基因种子，其中该转基因种子包含本发明的干扰RNA分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子、和/或组合物。在一些实施例中，该转基因种子是转基因玉米种子。
表达本发明的干扰RNA的转基因植物对被靶昆虫有害生物袭击具有耐受性或抗性。当该昆虫开始摄食这样一种转基因植物时，它也摄取所表达的dsRNA或siRNA。这可以妨碍昆虫进一步咬食植物组织或者甚至可以伤害或杀死昆虫。编码本发明的dsRNA或siRNA的核酸序列被插入到一种表达盒中，然后该表达盒被优选稳定地整合到该植物的基因组中。引入到植物基因组中的表达盒的这些核酸序列对于该植物而言是异源的，并且是天然存在的。跟据本发明所转化的植物可以是单子叶植物或双子叶植物，并且包括但不限于：玉米、小麦、大麦、黑麦、甘薯、豆、豌豆、菊苣、莴苣、卷心菜、花椰菜、西兰花、芜菁、萝卜、菠菜、芦笋、洋葱、大蒜、胡椒(pepper)、芹菜、小南瓜(squash)、大南瓜(pumpkin)、大麻、西葫芦、苹果、梨、榅桲、甜瓜、李子(plum)、樱桃、桃、油桃、杏、草莓、葡萄、覆盆子、黑莓、菠萝、鳄梨、番木瓜、芒果、香蕉、大豆、番茄、高粱、甘蔗、甜菜、向日葵、油菜籽、三叶草、烟草、胡萝卜、棉花、苜蓿、水稻、马铃薯、茄子、黄瓜、拟南芥属，以及木本植物，如针叶树以及落叶树。在另外的实施例中，该转基因植物是转基因玉米植物。
在转基因植物中该干扰RNA分子的表达是由包含在植物中发挥作用的启动子的调节序列所驱动的。启动子的选择将依赖于表达的时间和空间需要而变化，并且还依赖于昆虫目标种类而变化。因此，考虑了本发明的干扰RNA在叶、柄(stalk)或茎(stem)、穗、花序(例如穗状花序、圆锥花序、穗轴等)、根和/或幼苗中的表达。然而在许多情况下，寻求针对多于一种类型昆虫有害生物的保护，并且因此在多个组织中的表达是令人希望的。尽管已经显示来自双子叶植物的很多启动子在单子叶植物中是可操作的并且反之亦然，但理想的是选择双子叶植物启动子用于在双子叶植物中的表达，并且选择单子叶植物启动子用于在单子叶植物中的表达。然而，对所选择的启动子的起源并没有限制；足够的是它们在驱动dsRNA或siRNA在所希望的细胞中的表达中是操作性的。
适用于本发明的启动子包括但不限于组成性地驱动核苷酸序列的表达的那些启动子、在诱导时驱动表达的那些启动子、以及以组织或发育特异性方式驱动表达的那些启动子。这些不同类型的启动子在本领域是已知的。
在一些实施例中，可以使用组织特异性/组织优选的启动子。组织特异性或组织优选的表达模式包括但不限于绿色组织特异性或优选的、根特异性或优选的、茎特异性或优选的、以及花特异性或优选的。另外，可以使用在质体中起作用的启动子。在本发明的一些实施例中，可以使用诱导型启动子。在另外的方面中，本发明的核苷酸序列可以与如下启动子可操作地关联，该启动子是创伤诱导型或由有害生物或病原体侵染(例如，昆虫或线虫植物有害生物)进行的诱导型。
在本发明的一些实施例中，使用“最小启动子”或“基本启动子”。最小启动子能够募集并结合RNA聚合酶II复合体及其辅助蛋白，以允许转录起始和延伸。在一些实施例中，最小启动子被构建成仅包含来自转录因子的结合和感兴趣的核苷酸序列的转录所必需的选定启动子的核苷酸/核苷酸序列的启动子，这一感兴趣的核苷酸序列可操作地与包含但不局限于TATA盒序列的最小启动子相关联。在其他实施例中，最小启动子缺少募集并结合转录因子的cis序列，这些转录因子调节(例如，增强、抑制、赋予组织特异性，赋予诱导性或抑制性)转录。最小启动子通常被放置在待表达的核苷酸序列的上游(即，5’)。因此，可以选择来自与本发明一起可用的任何启动子的核苷酸/核苷酸序列用作最小启动子。
在一些实施例中，本发明的重组核酸分子可以是“表达盒”。如在此使用的，“表达盒”意指包含感兴趣的核苷酸序列(例如，本发明的核苷酸序列)的重组核酸分子，其中该核苷酸序列至少与控制序列(例如，启动子)可操作地关联。因此，本发明的一些实施例提供了被设计成表达编码本发明的dsRNA或siRNA的核苷酸序列的表达盒。以这种方式，例如，可操作地与本发明的一个或多个核苷酸序列相关联的一个或多个植物启动子可以提供于表达盒中，用于玉米植物、植物部分和/或植物细胞中的表达。
包含感兴趣的核苷酸序列的表达盒可以是嵌合的，意味着它的组分中的至少一种相对于它的其他组分中的至少一种是异源的。表达盒还可以是包含驱动其天然基因的天然启动子，然而已经以适用于异源表达的重组形式而获得的。表达盒的这种用途使它如此在其被引入的细胞中不是天然存在的。
表达盒还可以任选地包括在植物中具有功能性的转录和/或翻译终止区(即，终止区)。多种转录终止子是可供用于在表达盒中使用并且负责在超出感兴趣的异源核苷酸序列时的转录终止以及正确的mRNA聚腺苷酸化。该终止区对于该转录起始区可以是天然的，对于该可操作地连接的感兴趣的核苷酸序列可以天然的，对于该植物宿主可以是天然的，或者可以是源自另一种来源(即，对于该启动子、该感兴趣的核苷酸序列、该植物宿主、或其任意组合而言是外来的或异源的)。适当的转录终止子包括但不限于CAMV 35S终止子、tml终止子、胭脂碱合酶终止子和/或豌豆rbcs E9终止子。这些终止子可以在单子叶植物和双子叶植物二者中使用。此外，可以使用编码序列的天然转录终止子。
本发明的表达盒还可以包括用于选择性标记的核苷酸序列，该选择性标记可以用于选择转化的植物、植物部分和/或植物细胞。如在此使用，“选择性标记(selectablemarker)”意为是如下核苷酸序列，当该核苷酸序列表达时赋予一种不同的表型给表达该标记的植物、植物部分和/或植物细胞，并且因此允许此类经转化的植物、植物部分和/或植物细胞与不具有该标记的那些区别开来。这样的核苷酸序列可以编码选择性或筛选性标记，这取决于该标记是否赋予可以通过化学手段而被选择的性状，如通过使用选择剂(例如，抗生素、除草剂等)，或者取决于该标记是否仅是人们可以通过观察或测试而鉴别的性状，如通过筛选(例如，R基因座性状)。当然，适合的选择性标记的许多实例在本领域是已知的并且可以用于在此描述的表达盒中。
选择性标志的实例包括但不限于：一种编码neo或nptll的核苷酸序列，它赋予对卡那霉素、G418、以及类似物的抗性(Potrykus等人(1985)Mol.Gen.Genet.[分子与普通遗传学]199:183-188)；一种编码bar的核苷酸序列，它赋予对草丁膦的抗性；一种编码改变的5-烯醇丙酮莽草酸-3-磷酸(EPSP)合成酶的核苷酸序列，它赋予对草甘膦的抗性(Hinchee等人(1988)Biotech.[生物技术]6:915-922)；一种编码腈水解酶(如来自臭鼻克雷白氏杆菌(Klebsiella ozaenae)的bxn)的核苷酸序列，它赋予对溴草腈的抗性(Stalker等人(1988)Science[科学]242:419-423)；一种编码改变的乙酰乳酸合成酶(ALS)的核苷酸序列，它赋予对咪唑啉酮、磺酰脲或其他ALS-抑制化学品的抗性(欧洲专利申请号154204)；一种编码甲氨蝶呤-抗性的二氢叶酸还原酶(DHFR)的核苷酸序列(Thillet等人(1988)J.Biol.Chem.[生物化学杂志]263:12500-12508)；一种编码茅草枯脱卤素酶的核苷酸序列，它赋予对茅草枯的抗性；一种编码甘露糖-6-磷酸异构酶(也称为磷酸甘露糖异构酶(PMI))的核苷酸序列，它赋予代谢甘露糖的能力(美国专利号5,767,378和5,994,629)；一种编码改变的邻氨基苯甲酸盐合酶的核苷酸序列，它赋予对5-甲基色氨酸的抗性；和/或一种编码hph的核苷酸序列，它赋予对潮霉素的抗性。本领域技术人员能够选择用于在本发明的表达盒中使用的适合的选择性标记。
本发明的表达盒还可以包括对其他所希望的性状进行编码的多核苷酸。此类所希望的性状可以是赋予昆虫抗性或赋予线虫抗性的其他多核苷酸，或其他农业上所希望的性状。此类多核苷酸可以与核苷酸序列的任何组合叠加，以产生出具有所希望的表型的植物、植物部分或植物细胞。叠加的组合可以通过任何方法来产生，包括但不限于，通过任何常规的方法学的杂交育种植物或通过遗传转化。如果是通过遗传转化这些植物来进行叠加，则编码其他所希望的性状的核苷酸序列可以在任何时间并且以任何次序进行组合。例如，单个转基因可以包含多个表达盒，以致于多个表达盒被引入在单个基因组位置处的转化细胞的基因组中。可替代地，包含一种或多种所希望的性状的转基因植物可以用作通过后续转化而引入另外的性状的靶标。另外的核苷酸序列可以在共转化方案中与由表达盒的任何组合提供的本发明的核苷酸序列、核酸分子、核酸构建体、和/或其他组合物同时引入。例如，如果将引入两个核苷酸序列，则它们可以合并在分开的盒(反式)中或可以合并在相同的盒(顺式)上。这些核苷酸序列的表达可以通过相同的启动子或通过不同的启动子来驱动。进一步认识到的是，核苷酸序列可以在所希望的基因组位置处使用位点特异性重组系统进行叠加。参见，例如，国际专利申请公开号WO 99/25821；WO 99/25854；WO 99/25840；WO 99/25855；以及WO 99/25853。
因此，表达盒可以包括用于农艺性状的一种或多种多肽的编码序列，这些农艺性状的主要受益者是种子公司、种植者或谷粒加工者。感兴趣的多肽可以是由感兴趣的多核苷酸序列编码的任何多肽。适合用于在植物中生产的感兴趣的多肽的非限制性实例包括产生农艺学重要性状的那些多肽，这些性状如除草剂抗性(有时也称为“除草剂耐受性”)、病毒抗性、细菌病原体抗性、昆虫抗性、线虫抗性、和/或真菌抗性。参见，例如美国专利号5,569,823；5,304,730；5,495,071；6,329,504；和6,337,431。
适合于植物转化的载体说明于在本说明书的其他地方。对于农杆菌介导的转化，二元载体或携带至少一个T-DNA边界序列的载体是适合的，而对于直接基因转移，任何载体都是适合的，并且仅含有感兴趣的构建体的线性DNA也许是优选的。在直接基因转移的情况下，可以使用以单个DNA种类的转化或共转化(Schocher等人Biotechnology[生物技术]4:1093-1096(1986))。对于直接基因转移以及农杆菌介导的转化这二者，转化通常(但不是必需的)用一种选择性标记进行，这种选择性标记可以提供针对一种抗生素(卡那霉素、潮霉素、或甲氨蝶呤)或一种除草剂(Basta)的抗性。本发明的植物转化载体还可以包含其他选择性标记基因，例如，在美国专利5,767,378和5,994,629中所披露的，包含提供转基因植物阳性选择的基因(如磷酸甘露糖异构酶(pmi))(通过引用将其合并在此)或提供对除草剂草铵膦(草丁膦)耐受性的草铵膦乙酰转移酶(pat)。然而，选择性标记的选择对于本发明并不是至关重要的。
在其他实施例中，本发明的核酸序列被直接转化进入质体基因组中。在美国专利号5,451,513、5,545,817和5,545,818中，在PCT申请号WO 95/16783中，以及在McBride等人(1994)Proc.Nati.Acad.Sci.USA[美国国家科学院院刊]91,7301-7305中广泛描述了质体转化技术。基本的叶绿体转化技术涉及将位于选择性标记侧翼的经克隆的质体DNA区连同感兴趣的基因一起引入适合的靶组织中，这是例如使用生物射弹(biolistic)或原生质体转化(例如，氯化钙或PEG介导的转化)来进行的。这些1至1.5kb的侧翼区(被命名为靶向序列)促进了与质体基因组的同源重组，并且因而允许置换或修饰原质体(plastome)的特定区域。最初，将叶绿体16S rRNA和rps12基因的点突变(赋予对大观霉素和/或链霉素抗性)用作用于转化的选择性标记(Svab,Z.,Hajdukiewicz，P.和Maliga，P.(1990)Proc.Nati.Acad.Sci.USA[美国国家科学院院刊]87,8526-8530；Staub，J.M.和Maliga,P.(1992)Plant Cell[植物细胞]4,39-45)。这以大约每100次靶叶轰击大约1个的频率产生稳定的同质转化株。在这些标记之间克隆位点的存在允许建立质体靶向载体用于外来基因的引入(Staub,J.M.和Maliga,P.(1993)EMBO J.[欧洲分子生物学杂志]12,601-606)。大幅度提高转化效率是通过用显性的选择性标记(对大观霉素解毒酶氨基糖苷-3'-腺苷转移酶进行编码的细菌aadA基因)置换隐性的rRNA或r蛋白抗生素抗性基因而获得的(Svab,Z.和Maliga,P.(1993)Proc.Natl.Acad.Sci.[美国国家科学院院刊]90,913-917)。先前，这种标记已经被成功地用于莱茵衣藻这种绿藻的质体基因组的高频率转化(Goldschmidt-Clermont,M.(1991)Nucl.Acids Res.[核酸研究]19:4083-4089)。有用于质体转化的其他选择性标记在本领域是已知的，并且被包括在本发明的范围之内。典型地，转化之后需要大约15-20个细胞分裂循环以便达到同质状态。质体表达(其中基因通过同源重组被插入到在每个植物细胞中存在的所有数千个环状质体基因组的拷贝中)利用了超过核表达的基因的庞大的拷贝数目的优点，以便允许能够很容易超过总的可溶性植物蛋白的10％的表达水平。在一个优选的实施例中，将本发明的核酸序列插入至质体靶向的载体中并且转化至所希望的植物宿主的质体基因组中。获得了对于包含本发明的一种核酸序列的质体基因组同型的植物，并且这些植物优选地能够高度表达该核酸序列。
包含本发明的干扰RNA的转基因植物或种子还可以用一种杀昆虫剂或杀昆虫种子包衣进行处理，如描述于美国专利号5,849,320和5,876,739(通过引用结合在此)中。在本发明的杀昆虫剂或杀昆虫种子包衣以及转基因植物或种子能有效对抗同一靶昆虫(例如鞘翅目有害生物或根萤叶甲属靶有害生物)的情况下，该组合(i)在用于进一步增强本发明的组合物对抗该靶昆虫的活性的方法中以及(ii)在用于通过提供对抗该靶昆虫的又另一种作用机制而防止对本发明的组合物产生抗性的方法中是有用的。因此，本发明提供了一种增强对根萤叶甲属昆虫种群控制的方法，该方法包括提供本发明的转基因植物或种子并且向该植物或该种子施用本发明的杀昆虫剂或杀昆虫种子包衣。此类杀昆虫剂和/或杀昆虫种子包衣的实例包括但不限于，氨基甲酸酯、拟除虫菊酯、有机磷酸酯、friprole、新烟碱、有机氯化物、沙蚕毒素或其组合。在另一个实施例中，该杀昆虫剂或杀昆虫种子包衣选自下组，该组由以下组成：克百威，胺甲萘，灭多虫，联苯菊酯，七氟菊酯，氯菊酯，氟氯氰菊酯，λ-氯氟氰菊酯，氯氰菊酯，溴氰菊酯，毒死蜱，氯氧磷，乐果，灭线磷，马拉硫磷，甲基对硫磷，甲拌磷，特丁磷，叔丁嘧啶磷(tebupirimiphos)，氟虫腈，啶虫脒，吡虫啉，噻虫啉，噻虫嗪，硫丹，杀虫磺，及其组合。包含此类杀昆虫剂和杀昆虫种子包衣的商业产品包括但不限于(卡巴呋喃)、(灭多虫、灭多威、mesomile)、(胺甲萘)、(联苯菊酯)、(七氟菊酯)、(氯氰菊酯)、(氯氰菊酯)、Delta (溴氰菊酯)、(高效氯氟氰菊酯)、(苄氯菊酯)、(苄氯菊酯)、(联苯菊酯)、(联苯菊酯)、(七氟菊酯)、(高效氯氟氰菊酯)、(毒死蜱)、(氯氧磷)、(灭克磷)、(甲拌磷)、(甲拌磷、烯虫酯)、(甲拌磷)、(特丁磷)、(乐果)、异氯硫磷、(氟虫腈)、(噻虫嗪)、(吡虫啉)、(吡虫啉)、(噻虫胺)以及(氟氯氰菊酯、嘧丙磷)。
本发明的组合物还可以与其他生物控制试剂组合，以增强对鞘翅目昆虫或根萤叶甲属昆虫种群的控制。因此，通过提供一种转基因植物，本发明提供了一种增强对鞘翅目昆虫种群或根萤叶甲属昆虫种群的控制的方法，该转基因植物产生本发明的干扰RNA，并且进一步包含一种编码第二杀昆虫剂的多核苷酸。该第二杀昆虫剂可以是源自苏云金芽孢杆菌的杀昆虫蛋白。苏云金芽孢杆菌杀昆虫蛋白可以是许多杀昆虫蛋白中的任何一种，包括但不限于Cry1蛋白、Cry3蛋白、Cry7蛋白、Cry8蛋白、Cry11蛋白、Cry22蛋白、Cry23蛋白、Cry36蛋白、Cry37蛋白、Cry34蛋白连同Cry35蛋白、二元杀昆虫蛋白CryET33和CryET34、二元杀昆虫蛋白TIC100和TIC101、二元杀昆虫蛋白PS149B1、VIP、TIC900或相关蛋白、TIC901、TIC1201、TIC407、TIC417、修饰的Cry3A蛋白、或杂合蛋白或由任何前述杀昆虫蛋白制成的嵌合体。在其他实施例中，该苏云金芽孢杆菌杀昆虫蛋白选自下组，该组由以下组成：Cry3Bb1、Cry34Ab1连同Cry35Ab1、mCry3A以及eCry3.1Ab。
在其他实施例中，该转基因植物可以产生本发明的干扰RNA和源自苏云金芽孢杆菌以外来源的第二杀昆虫剂。该第二杀昆虫剂可以是选自下组的试剂，该组包含：马铃薯糖蛋白、蛋白酶、蛋白酶抑制剂、几丁质酶、脲酶、α-淀粉酶抑制剂、成孔蛋白、凝集素、工程化抗体或抗体片段、蜡样芽孢杆菌杀昆虫蛋白、致病杆菌属(如嗜线虫致病杆菌或伯氏致病杆菌)杀昆虫蛋白、光杆菌属(如发光杆菌或非共生光杆菌)杀昆虫蛋白、侧孢短芽孢杆菌杀昆虫蛋白、球形赖氨酸芽胞杆菌杀昆虫蛋白、色杆菌属杀昆虫蛋白、噬虫霉耶尔森菌杀昆虫蛋白、乳状芽孢杆菌杀昆虫蛋白、梭菌属(如双酶梭菌)杀昆虫蛋白、和木质素。在其他实施例中，该第二试剂可以是至少一种源自来自发光杆菌、致病杆菌、沙雷氏菌或耶尔森氏菌的杀昆虫毒素复合物(Tc)的杀昆虫蛋白。在其他实施例中，该杀昆虫蛋白可以是源自杀昆虫细菌(如光杆菌属)的ADP-核糖基转移酶。在其他实施例中，该杀昆虫蛋白可以是VIP蛋白，如来自蜡样芽孢杆菌的VIP1或VIP2。在仍其他实施例中，该杀昆虫蛋白可以是源自杀昆虫细菌的二元毒素，如来自侧孢芽孢杆菌的ISP1A和ISP2A或来自球形赖氨酸芽胞杆菌的BinA和BinB。在仍其他实施例中，该杀昆虫蛋白可以是工程化的或可以是任何前述杀昆虫蛋白的杂合体或嵌合体。
在另一个实施例中，该转基因植物和转基因种子是玉米植物或玉米种子。在另一个实施例中，该转基因玉米植物通过包含本发明的dsRNA的第一转基因玉米植物与包含选自下组的转基因事件的转基因玉米植物杂交而提供，该组由以下各项组成：MIR604、事件5307、DAS51922-7、MON863以及MON88017。
即使在该杀昆虫剂或杀昆虫种子包衣能有效对抗一种不同的昆虫的情况下，该杀昆虫剂或杀昆虫种子包衣对于扩展昆虫控制的范围是有用的，例如通过将一种具有对抗鳞翅目昆虫活性的杀昆虫剂或杀昆虫种子包衣加入本发明的转基因植物或种子(具有对抗鞘翅目昆虫的活性)中，所处理的植物或包衣的转基因种子控制鳞翅目和鞘翅目有害生物两者。
在另外的实施例中，本发明涵盖来自本发明的转基因植物、种子或其部分的生物样品，其中该样品包含作为本发明的dsRNA或编码本发明的dsRNA的至少一条链的核酸。在其他实施例中，本发明涵盖一种衍生自本发明的转基因植物、种子或其部分的商品产品。在一些实施例中，该商品产品选自下组，该组由以下组成：整个或经处理的种子、豆类、谷物、籽粒、壳、粉、粗碾去壳谷类、面粉、糖、糖、淀粉、蛋白质浓缩物、蛋白质分离物、蜡、油、提取物、汁、浓缩物、液体、糖浆、饲料、青贮饲料、纤维、纸或其他从植物生产的食物或产品。在其他实施例中，该生物样品或商品产品对昆虫具有毒性。在其他实施例中，该转基因植物是转基因玉米植物。
本发明进一步涵盖一种控制鞘翅目昆虫或根萤叶甲属昆虫的方法，该方法包括使该根萤叶甲属昆虫与一种作为本发明的干扰RNA分子或能够产生本发明的干扰RNA分子的核酸分子接触，该干扰RNA分子用于抑制靶基因在该昆虫中的表达，从而控制该鞘翅目昆虫或根萤叶甲属昆虫。在一些实施例中，该靶基因包含如下编码序列，该编码序列(i)至少与SEQ ID NO:1-30，SEQ ID NO:91-120，SEQ ID NO:271-273，SEQ ID NO:277-279，SEQ IDNO:283-297，SEQ ID NO:313-317，SEQ ID NO:372、377、379，SEQ ID NO:381-388，SEQ IDNO:399；或SEQ ID NO:400的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段具有至少80％同一性、至少85％同一性、至少86％同一性、至少87％同一性、至少88％同一性、至少89％同一性、至少90％同一性、至少91％同一性、至少92％同一性、至少93％同一性、至少94％同一性、至少95％同一性、至少96％同一性、至少97％同一性、至少98％同一性、至少99％同一性、或100％同一性；(ii)至少包含SEQ ID NO:1-30，SEQ ID NO:91-120，SEQ ID NO:271-273，SEQID NO:277-279，SEQ ID NO:283-297，SEQ ID NO:313-317，SEQ ID NO:372、377、379，SEQID NO:381-388，SEQ ID NO:399；或SEQ ID NO:400的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段；(iii)至少包含编码由SEQ ID NO:1-30，SEQ ID NO:91-120，SEQ ID NO:271-273，SEQ ID NO:277-279，SEQ ID NO:283-297，SEQ ID NO:313-317，SEQ ID NO:372、377、379，SEQ ID NO:381-388，SEQ ID NO:399；或SEQ ID NO:400编码的氨基酸序列的核苷酸序列的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或者(iv)可以在严格条件下与选自下组的多核苷酸杂交，该组由以下组成：SEQ ID NO:1-30，SEQ ID NO:91-120，SEQ ID NO:271-273，SEQ ID NO:277-279，SEQ ID NO:283-297，SEQ ID NO:313-317，SEQ ID NO:372、377、379，SEQ ID NO:381-388，SEQ ID NO:399；或SEQ ID NO:400，及其互补序列。在一些实施例中，该靶基因编码序列包含SEQ ID NO:1-30，SEQ ID NO:91-120，SEQ ID NO:271-273，SEQ ID NO:277-279，SEQ IDNO:283-297，SEQ ID NO:313-317，SEQ ID NO:372、377、379，SEQ ID NO:381-388，SEQ IDNO:399；或SEQ ID NO:400。在其他实施例中，本发明的干扰RNA分子与可从在此描述的根萤叶甲属靶基因转录的mRNA多核苷酸的一部分互补。
在控制鞘翅目昆虫有害生物或根萤叶甲属昆虫有害生物的方法的一些实施例中，本发明的干扰RNA分子包含至少一种dsRNA，其中该dsRNA是包含退火的互补链的双链RNA的区域，其中的一条链包含具有至少19个连续核苷酸的序列，该序列(i)至少与SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列具有至少80％同一性、至少85％同一性、至少86％同一性、至少87％同一性、至少88％同一性、至少89％同一性、至少90％同一性、至少91％同一性、至少92％同一性、至少93％同一性、至少94％同一性、至少95％同一性、至少96％同一性、至少97％同一性、至少98％同一性、至少99％同一性、或100％同一性；(ii)至少包含SEQ ID NO:121-210，SEQ IDNO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列；(iii)至少包含编码由SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400编码的氨基酸序列的核苷酸序列的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列，或者(iv)可以在严格条件下与选自下组的多核苷酸杂交，该组由以下组成：SEQ ID NO:121-210，SEQ IDNO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400，及其互补序列。
在控制鞘翅目昆虫有害生物或根萤叶甲属昆虫有害生物的方法的一些实施例中，该干扰RNA分子包含SEQ ID NO:181-210的从18、19、20、或21个连续核苷酸到至少约300个连续核苷酸，基本由其组成或由其组成。在其他实施例中，本发明的干扰RNA包含由N至N+20个核苷酸组成的SEQ ID NO:181-210的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成。例如，本发明的干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:181的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQID NO:181的核苷酸1至核苷酸776。换言之，被靶向的mRNA的该部分包含776个SEQ ID NO:181的21个连续核苷酸(即21-mers)的子序列中的任一个，或其任何互补序列。将认识到的是，这些776个21个连续核苷酸的子序列包括来自SEQ ID NO:121和SEQ ID NO:151的所有可能的21个连续核苷酸的子序列，以及它们的互补序列，因为SEQ ID NO 121、151和181都针对相同的靶标，即BPA_15366。类似地将认识到的是，SEQ ID NO:181-210的所有21-mer子序列及其所有互补序列子序列包括SEQ ID NO:121-180和SEQ ID NO:373的所有可能的21个连续核苷酸的子序列，及其互补序列子序列。
类似地，本发明的干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:182的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:182的核苷酸1至核苷酸771。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ IDNO:183的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQID NO:183的核苷酸1至核苷酸2907。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:184的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:184的核苷酸1至核苷酸1600。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:185的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:185的核苷酸1至核苷酸2410。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:186的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:186的核苷酸1至核苷酸2802。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:187的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:187的核苷酸1至核苷酸3681。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:188的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:188的核苷酸1至核苷酸651。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:189的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ IDNO:189的核苷酸1至核苷酸673。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:190的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:190的核苷酸1至核苷酸2664。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:191的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:191的核苷酸1至核苷酸438。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:192的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:192的核苷酸1至核苷酸2458。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:193的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:193的核苷酸1至核苷酸3254。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:194的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:194的核苷酸1至核苷酸3632。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:195的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:195的核苷酸1至核苷酸7611。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:196的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ IDNO:196的核苷酸1至核苷酸1008。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:197的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:197的核苷酸1至核苷酸2992。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:198的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:198的核苷酸1至核苷酸1192。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:199的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:199的核苷酸1至核苷酸7626。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:200的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:200的核苷酸1至核苷酸2580。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:201的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:201的核苷酸1至核苷酸4628。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:202的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ IDNO:202的核苷酸1至核苷酸1557。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:203的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:203的核苷酸1至核苷酸1019。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:204的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:204的核苷酸1至核苷酸677。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:205的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:205的核苷酸1至核苷酸764。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:206的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:206的核苷酸1至核苷酸1830。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:207的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:207的核苷酸1至核苷酸3225。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:208的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:208的核苷酸1至核苷酸1003。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQID NO:209的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:209的核苷酸1至核苷酸1419。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:210的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:210的核苷酸1至核苷酸5206。本发明的另一个干扰RNA分子包含如下dsRNA，该dsRNA包含SEQ ID NO:374的任何21-mer子序列，或其任何互补序列，基本由其组成或由其组成，其中N是SEQ ID NO:374的核苷酸1至核苷酸7112。
在控制根萤叶甲属昆虫有害生物的方法的一些实施例中，该根萤叶甲属昆虫选自下组，该组由以下组成：巴氏根萤叶甲(北方玉米根虫)，西方玉米根萤叶甲(西方玉米根虫)，黄瓜十一星叶甲食根亚种(南方玉米根虫)，黄瓜条根萤叶甲(带状黄瓜甲虫)，黄瓜十一星叶甲(西方斑点黄瓜甲虫)，斯格尼根萤叶甲(3斑叶甲)，南美叶甲(菊花甲虫)以及墨西哥玉米根萤叶甲(墨西哥玉米根虫)。
在控制鞘翅目昆虫有害生物或根萤叶甲属昆虫有害生物的方法的其他实施例中，该接触包括(a)种植转基因种子，该转基因种子能够产生一种表达该核酸分子的转基因植物，其中该昆虫摄食该转基因植物或其部分；或(b)将包含该核酸分子的组合物施用至种子或植物或其部分，其中该昆虫摄食该种子、该植物或其部分。在一些实施例中，该转基因种子和该转基因植物是玉米种子或玉米植物。在其他施例中，该种子或植物是玉米种子或玉米植物。
本发明还涵盖一种控制根萤叶甲属昆虫的方法，该方法包括使该根萤叶甲属昆虫与一种作为本发明的干扰RNA分子或能够产生本发明的干扰RNA分子的核酸分子接触，该干扰RNA分子用于抑制靶基因在根萤叶甲属昆虫中的表达，并且还使该根萤叶甲属昆虫至少与用于控制根萤叶甲属的第二杀昆虫剂接触，其中所述第二杀昆虫剂包含苏云金芽孢杆菌杀昆虫蛋白，从而控制该根萤叶甲属昆虫。本发明还涵盖一种控制植物上的根萤叶甲属昆虫有害生物的方法，该方法包括向所述植物局部施用包含本发明的干扰RNA和至少用于控制根萤叶甲属的第二杀昆虫剂的杀有害生物剂组合物，其中所述第二杀昆虫剂不包含苏云金芽孢杆菌杀昆虫蛋白，并且在所述根萤叶甲属昆虫的饲料中提供所述植物。本发明还涵盖一种方法，其中该第二杀昆虫剂包含马铃薯糖蛋白、蛋白酶、蛋白酶抑制剂、脲酶、α-淀粉酶抑制剂、成孔蛋白、凝集素、工程化抗体或抗体片段、或几丁质酶。该第二杀昆虫剂可以是蜡样芽孢杆菌杀昆虫蛋白、致病杆菌属杀昆虫蛋白、光杆菌属杀昆虫蛋白、侧孢短芽孢杆菌杀昆虫蛋白、球形赖氨酸芽胞杆菌杀昆虫蛋白、色杆菌属杀昆虫蛋白、噬虫霉耶尔森菌杀昆虫蛋白、乳状芽孢杆菌杀昆虫蛋白、或梭菌属杀昆虫蛋白。
本发明还涵盖一种减少转基因植物上的成虫鞘翅目昆虫种群或成虫根萤叶甲属昆虫种群的方法，该转基因植物表达Cry蛋白、杂种Cry蛋白、或修饰的Cry蛋白，该方法包括在该转基因植物中表达一种作为本发明的干扰RNA分子或能够产生本发明的干扰RNA分子的核酸分子，从而减少该成虫鞘翅目昆虫种群或成虫根萤叶甲属昆虫种群，该干扰RNA分子能够抑制如在此描述的靶基因在成虫昆虫中的表达。
在一些实施例中，本发明涵盖一种降低可从如在此描述的靶基因转录的靶mRNA在鞘翅目昆虫或根萤叶甲属昆虫中的水平的方法，该方法包括使该昆虫与一种包含本发明的干扰RNA分子的组合物接触，其中该干扰RNA分子降低该靶RNA在昆虫的细胞中的水平。在一些实施例中，本方法的干扰RNA包含至少一种dsRNA，其中该dsRNA是包含退火的互补链的双链RNA的区域，其中的一条链包含具有至少19个连续核苷酸的序列，该序列(i)至少与SEQID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ IDNO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列具有至少80％同一性、至少85％同一性、至少86％同一性、至少87％同一性、至少88％同一性、至少89％同一性、至少90％同一性、至少91％同一性、至少92％同一性、至少93％同一性、至少94％同一性、至少95％同一性、至少96％同一性、至少97％同一性、至少98％同一性、至少99％同一性、或100％同一性；(ii)至少包含SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQID NO:373、374、378、380、389-396、399、400的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列；(iii)至少包含编码由SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400编码的氨基酸序列的核苷酸序列的19、20、21、22、23、24、25、26、27、28、29、30、35、40、45、50、55、60、65、70、75、80、85、90、95、100、110、120、130、140、150、160、170、180、190、200、210、220、230、240、250、260、270、280、290、或300个连续核苷酸的片段，或其互补序列，或者(iv)可以在严格条件下与选自下组的多核苷酸杂交，该组由以下组成：SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400，及其互补序列，其中该干扰RNA分子对靶鞘翅目昆虫或根萤叶甲属昆虫具有杀昆虫活性。在另一个实施例中，通过靶昆虫摄食该组合物来实现接触。在其他实施例中，由该靶mRNA编码的蛋白质的产生被减少。在其他实施例中，该靶蛋白包含与SEQ ID NO:241-270或SEQ ID NO:376具有至少约80％、至少约85％、至少约90％、至少约91％、至少约92％、至少约93％、至少约94％、至少约95％、至少约96％、至少约97％、至少约98％、或至少约99％同一性的氨基酸。在其他实施例中，该靶蛋白包含SEQ ID NO:241-270或SEQ ID NO:376。在其他实施例中，该干扰RNA通过表达该干扰RNA的转基因生物体与鞘翅目昆虫或根萤叶甲属昆虫接触。在其他实施例中，该转基因生物体是一种转基因植物、转基因微生物、转基因细菌或转基因内寄生菌。在其他实施例中，通过将可接受的农用载体中的干扰RNA局部地施用至昆虫摄食的植物或植物部分来使该干扰RNA与鞘翅目昆虫或根萤叶甲属昆虫接触。在一些实施例中，减少可从在此描述的靶基因转录的靶mRNA的水平的干扰RNA对该鞘翅目昆虫或根萤叶甲属昆虫而言是致死的。在一些实施例中，该根萤叶甲属昆虫选自下组，该组由以下组成：巴氏根萤叶甲(北方玉米根虫)，西方玉米根萤叶甲(西方玉米根虫)，黄瓜十一星叶甲食根亚种(南方玉米根虫)，黄瓜条根萤叶甲(带状黄瓜甲虫)，黄瓜十一星叶甲(西方斑点黄瓜甲虫)，斯格尼根萤叶甲(3斑叶甲)，南美叶甲(菊花甲虫)以及墨西哥玉米根萤叶甲(墨西哥玉米根虫)。
在一些实施例中，本发明涵盖一种赋予植物或其部分对鞘翅目昆虫耐受性或根萤叶甲属昆虫耐受性的方法，该方法包括向该植物或其部分中引入本发明的干扰RNA分子、dsRNA分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子、和/或组合物，其中本发明的dsRNA分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子、和/或组合物对于该昆虫是有毒的，从而赋予该植物或其部分对该鞘翅目昆虫或根萤叶甲属昆虫的耐受性。在其他实施例中，通过转化一种植物细胞并且自该转化的植物细胞产生该转基因植物来进行该引入步骤。在仍其他实施例中，通过共同培育两种植物来进行该引入步骤。
在其他实施例中，本发明涵盖一种减少被根萤叶甲属昆虫摄食的植物的根损害的方法，该方法包括向该植物的细胞中引入本发明的干扰RNA分子、dsRNA、核酸分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子、和/或组合物，其中本发明的dsRNA、核酸分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子、和/或组合物对于根萤叶甲属昆虫是有毒的，从而减少对该植物的根损害。在其他实施例中，通过转化植物细胞并且自该转化的植物细胞产生该转基因植物来进行该引入步骤。在仍其他实施例中，通过共同培育两种植物来进行该引入步骤。
在仍其他的实施例中，本发明涵盖一种产生对鞘翅目昆虫或根萤叶甲属昆虫具有毒性的转基因植物细胞的方法，该方法包括向植物细胞中引入本发明的干扰RNA分子、dsRNA、核酸分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子、和/或组合物，从而产生与对照植物细胞相比，对该昆虫具有毒性的转基因植物细胞。在一些实施例中，本发明涵盖多个由这一方法产生的转基因植物细胞。在其他实施例中，使该多个转基因植物细胞在包括天然日光的条件下生长。在其他实施例中，通过转化植物细胞并且自该转化的植物细胞产生该转基因植物来进行该引入步骤。在仍其他实施例中，通过共同培育两种植物来进行该引入步骤。
在一些实施例中，本发明涵盖一种产生对鞘翅目或根萤叶甲属昆虫摄食损害具有增强的耐受性的转基因植物的方法，该方法包括向植物中引入本发明的干扰RNA分子、dsRNA、核酸分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子、和/或组合物，从而产生与对照植物相比，对鞘翅目或根萤叶甲属昆虫摄食损害具有增强的耐受性的转基因植物。在其他实施例中，通过转化一种植物细胞并且自该转化的植物细胞产生该转基因植物来进行该引入步骤。在仍其他实施例中，通过共同培育两种植物来进行该引入步骤。
在一些实施例中，本发明涵盖一种为玉米种植者提供控制玉米作物中的鞘翅目昆虫有害生物种群或根萤叶甲属昆虫有害生物种群的手段的方法，该方法包括(a)向该种植者销售或为其提供包含本发明的干扰RNA、核酸分子、核酸构建体、嵌合核酸分子、人工植物微小RNA前体分子、和/或组合物的转基因玉米种子；并且(b)向该种植者做广告宣传该转基因玉米种子产生控制鞘翅目或根萤叶甲属有害生物种群的转基因玉米植物。
在一些实施例中，本发明涵盖一种鉴定靶基因的方法，该方法用作RNAi策略用于在鞘翅目植物有害生物中产生RNAi用于控制植物有害生物，所述方法包括以下步骤：a)产生具有选自下组的序列的引物对，该组包含SEQ ID NO:31-90、397、398，或其互补序列，或由其组成；b)扩增来自该植物有害生物的核酸样品的直系同源靶标；c)鉴定直系同源靶基因的序列；d)产生干扰RNA分子，其中该RNA包含至少一种dsRNA，其中该dsRNA是包含退火的互补链的双链RNA的区域，其中的一条链包含具有至少19个连续核苷酸的序列，该序列与鞘翅目靶基因中的靶核苷酸序列是至少部分互补的；以及e)确定该干扰RNA分子是否对该植物有害生物具有杀昆虫活性。如果该干扰RNA对鞘翅目有害生物具有杀昆虫活性，则已鉴定出用于控制植物有害生物的靶基因。在一些实施例中，该植物有害生物是鞘翅目植物有害生物。
实例
通过参考以下具体的实例可以进一步描述本发明。这些实例仅仅是出于说明性目的而提供的，并且并不旨在进行限制，除非另外指明。实例1.鉴定西方玉米根萤叶甲中的RNAi基因靶标
这一实例描述了来自根萤叶甲属昆虫的RNAi靶基因和编码序列的克隆和测序。
西方玉米根萤叶甲焦磷酸测序文库制备和测序
基本根据制造商的说明，在454平台(454生命科学公司(454Life Sciences)，布兰福德，康涅狄格州)上通过焦磷酸测序对全身新生西方玉米根萤叶甲(西方玉米根虫(WCR))转录组进行测序。将所得测定序列(read)(即，核酸序列的短片段)进行修整并使用MIRA组装器组装成重叠群(参见例如，Chevreux等人2004Genome Res.[基因组研究]14:1147-1159，通过引用结合在此)。
鉴定来自根萤叶甲属的最重要的靶基因
通过BLAST将组装的重叠群与已知的致死基因和其他生物中的等位基因进行比较，基于以下公开的披露对所述基因进行鉴定，包括网站线虫数据库(wormbase.org)中的那些和Boutros等人(2004,Science[科学]303:832-835)。根据该分析，鉴定了4,608个靶基因。这些靶基因中的每一个都是非冗余的并且已知具有一个或多个致死的等位基因，或者已知当在线虫、果蝇或两者中被RNAi靶向时导致致死性。因此，这些靶标中的每一个都被认为是必需的。预期的是，显著大比例的这些靶基因将在WCR中具有杀昆虫效果。令人惊讶的是，事实并非如此。
在384孔自动化文库合成平台上产生4,608个靶标的dsRNA。使用引物设计工具Primer3自动设计测试的所有dsRNA样品，以基于每种靶基因的编码序列合成约500bp-600bp的dsRNA片段。如果编码序列的大小不允许500bp片段，则设计较小的片段。将这些样品在24孔WCR测定中以一种浓度(100ng dsRNA/cm2，即190ng dsRNA/孔)进行筛选，每孔具有10个L2WCR幼虫。10天之后对死亡率进行评分。候选物命中的截止值为69％死亡率。在4,608个候选dsRNA靶标中，鉴定了183个靶基因。这些结果是令人惊讶的，因为本领域技术人员预期这4,608个候选靶标中有更大数量的靶标在生物测定中赋予毒性。
在第一测定中，从1μg dsRNA/孔开始，以10倍稀释系列在实验室生物测定中测试7个靶标。使用RNA处理的人工饲料法进行生物测定。简言之，将修改自Marrone等人1985(J.Econ.Entomol.[经济昆虫学杂志]78:290-293)的饲料的熔化的人工饲料倾倒进48孔板的每个孔中并允许凝固。将dsRNA分子稀释至适当浓度，这样使得将20μl的溶液添加至48孔板的一半的孔中的饲料的表面，最终覆盖浓度为每孔1μg、0.1μg、0.01μg和0.001μg。向每个孔中添加一个或两个WCR幼虫，以具有24至48个重复幼虫/dsRNA测试浓度。将每个48孔板保持在约26℃，伴随16小时:8小时的光照:黑暗光周期。在侵染后第1、2、3、4、6和7天记录死亡率。将被设计成靶向绿色荧光蛋白(GFP)的dsRNA用作所有生物测定中的阴性对照，并且将被设计成靶向WCR的泛素基因的dsRNA用作阳性对照。根据该测定，BPA_46378(α-snap)被确认为阳性。四种候选物未被确认为阳性。
在确认筛选中同时测试其他176个靶基因。在自动化文库合成平台上产生176个靶标的dsRNA，以及阳性和阴性对照dsRNA。在该筛选下对BPA_46378也进行了测试。使用RNA处理的人工饲料法进行生物测定。简言之，将修改自Marrone等人1985(J.Econ.Entomol.[经济昆虫学杂志]78:290-293)的饲料的熔化的人工饲料倾倒进48孔板的每个孔中并允许凝固。将dsRNA分子稀释至适当浓度，这样使得将20μl的溶液添加至48孔板的一半的孔中的饲料的表面，最终覆盖浓度为每孔0.5μg dsRNA。向每个孔中添加一个或两个WCR幼虫，以具有24至48个重复幼虫/测试dsRNA。将每个48孔板保持在约26℃和16:8的光照:黑暗光周期中。处理后7天记录死亡率。
示于表1中的结果发现最初鉴定的176种dsRNA分子中的29种dsRNA分子被确认为对西方玉米根萤叶甲(西方玉米根虫)具有高毒性，除了被重新确认的α-snap靶标外。SEQID NO:1-30是筛选中鉴定的每种毒性靶基因的核酸片段的核苷酸序列。SEQ ID NO:31-90、397、398及其互补序列是用于合成筛选中鉴定的每种靶基因的核酸片段的引物对的核苷酸序列。SEQ ID NO:91-120是通过该筛选鉴定的每种靶基因的全长编码序列的核苷酸序列。
先前已经表明，根萤叶甲属的某些基因可用于RNAi介导的昆虫控制。参见美国专利公开号2007/0124836(其披露了906个序列)和美国专利号7,612,194(其披露了9,112个序列)。然而，如在此证明的，任何给定基因靶标通过RNAi方法赋予毒性的能力都无法被预测，并且只能按照经验确定。Narva等人(美国公开号2015/0322456)已经达成了类似的结论。本发明鉴定了30种靶基因，其中每种靶基因提供令人惊讶和意想不到的对根萤叶甲属的优异控制。
表1.处理后7天dsRNA对抗西方玉米根萤叶甲的活性
实例2.dsRNA(4种浓度)对抗西方玉米根萤叶甲-DRC的活性
这一实例描述了测试本发明的dsRNA对抗西方玉米根萤叶甲(WCR)的生物活性。
从1μg dsRNA/孔开始，以10倍稀释系列在实验室生物测定中测试上述30种dsRNA分子对抗WCR的毒性。使用RNA处理的人工饲料法进行生物测定。简言之，将修改自Marrone等人1985(J.Econ.Entomol.[经济昆虫学杂志]78:290-293)的饲料的熔化的人工饲料倾倒进48孔板的每个孔中并允许凝固。将合成的dsRNA分子稀释至适当浓度，这样使得将20μl的溶液添加至48孔板的一半的孔中的饲料的表面，最终覆盖浓度为每孔1μg、0.1μg、0.01μg和0.001μg。向每个孔中添加一个或两个WCR幼虫，以具有24至48个重复幼虫/dsRNA测试浓度。将每个48孔板保持在约26℃和16:8的光照:黑暗光周期中。在侵染后第1、2、3、4、6和7天记录死亡率。将被设计成靶向GFP的dsRNA用作阴性对照，并且将被设计成靶向WCR的泛素基因的dsRNA用作阳性对照。
示于表2中的结果证明，被设计成靶向可从WCR基因转录的mRNA的30种dsRNA分子对WCR具有毒性至高毒性。在对GFPdsRNA的对照死亡率进行校正后，通过曲线拟合分析计算估计的LT50和LC50。LT50代表致死时间，以获得测试昆虫中50％的死亡率。LC50代表dsRNA的浓度，该浓度导致50％的测试昆虫死亡。在表2中，第7天的死亡率％基于1μg dsRNA/孔。LT50基于使用1μg dsRNA/天并以天数进行测量。LC50以μg dsRNA/孔进行测量。
表2.处理后7天dsRNA对抗西方玉米根萤叶甲的活性
基于这些结果，优选一个亚组的靶标进行进一步研究。这些靶标的结果如下所示。
实例3.dsRNA对抗西方玉米根萤叶甲的活性
这一实例描述了测试一个亚组的本发明的经鉴定的靶dsRNA对抗西方玉米根萤叶甲(WCR)的生物活性。
以0.5μg dsRNA/孔开始，以3倍稀释系列在实验室生物测定中测试上述dsRNA分子对抗WCR的毒性。使用RNA处理的人工饲料法进行生物测定。简言之，将修改自Marrone等人1985(J.Econ.Entomol.[经济昆虫学杂志]78:290-293)的饲料的熔化的人工饲料倾倒进48孔板的每个孔中并允许凝固。将dsRNA分子稀释至适当浓度，这样使得将20μl的溶液添加至48孔板的一半的孔中的饲料的表面，最终覆盖浓度为每孔0.5μg、0.16μg、0.05μg、0.02μg、0.006μg、0.002μg、0.0007μg和0.0002μg。向每个孔中添加一个或两个WCR幼虫，以具有24至48个重复幼虫/dsRNA测试浓度。将每个48孔板保持在约26℃和16:8的光照:黑暗光周期中。在侵染后第1、2、3、4、6和7天记录死亡率。将被设计成靶向GFP的dsRNA用作阴性对照，并且将被设计成靶向WCR的泛素基因的dsRNA用作阳性对照。
示于表3中的结果证明，被设计成靶向可从WCR基因转录的mRNA的dsRNA分子对WCR具有毒性至高毒性。在对GFP dsRNA的对照死亡率进行校正后，通过曲线拟合分析计算估计的LT50和LC50。LT50代表致死时间，以获得测试昆虫中50％的死亡率。LC50代表dsRNA的浓度，该浓度导致50％的测试昆虫死亡。在表3中，第7天的死亡率％基于0.5μg dsRNA/孔。LT50基于使用0.5μg dsRNA/天并以天数进行测量。LC50以μg dsRNA/孔进行测量。这些结果确认了候选靶标的毒性。
表3.dsRNA对抗西方玉米根萤叶甲的活性
实例4.dsRNA对抗黄瓜十一星叶甲食根亚种的活性
这一实例描述了测试本发明的dsRNA对抗黄瓜十一星叶甲食根亚种(南方玉米根虫(SCR))的生物活性。
以0.5μg dsRNA/孔开始，以10倍稀释系列在实验室生物测定中测试上述dsRNA分子对抗SCR的毒性。使用RNA处理的人工饲料法进行生物测定。简言之，将修改自Marrone等人1985(J.Econ.Entomol.[经济昆虫学杂志]78:290-293)的饲料的熔化的人工饲料倾倒进48孔板的每个孔中并允许凝固。将合成的dsRNA分子稀释至适当浓度，这样使得将20μl的溶液添加至每个孔中的饲料的表面，3x稀释步骤中的最终8种覆盖浓度的浓度系列为从0.5μg/孔低至0.00022μg/孔。向每个孔中添加一个或两个SCR幼虫，以具有24至48个重复幼虫/dsRNA测试浓度。将每个48孔板保持在约26℃和16:8的光照:黑暗光周期中。在侵染后第1、2、3、4、5、6、7、8、9、10、和14天记录死亡率。将被设计成靶向GFP的dsRNA用作阴性对照，并且将被设计成靶向西方玉米根萤叶甲(Dv)泛素基因和黄瓜十一星叶甲食根亚种(Du)泛素基因的dsRNA用作阳性对照。
在对GFP dsRNA的对照死亡率进行校正后，通过曲线拟合分析计算估计的LT50和LC50。LT50代表致死时间，以获得测试昆虫中50％的死亡率。LC50代表dsRNA的浓度，该浓度导致50％的测试昆虫死亡。在表4中，第14天的死亡率％基于0.5μg dsRNA/孔。LT50基于使用0.5μg dsRNA/天并以天数进行测量。LC50以μg dsRNA/孔进行测量。示于表4中的结果证明，被设计成靶向可从西方玉米根萤叶甲(WCR)基因转录的mRNA的dsRNA分子也对黄瓜十一星叶甲食根亚种(SCR)具有毒性。这表明本发明的靶标也是SCR的合适靶标，这样使得基于天然SCR mRNA的dsRNA分子也对SCR和其他根萤叶甲属具有毒性。
表4.处理后14天dsRNA对抗黄瓜十一星叶甲食根亚种的活性
实例5.dsRNA对抗巴氏根萤叶甲的活性
这一实例描述了测试本发明的dsRNA对抗巴氏根萤叶甲(北方玉米根虫(NCR))的生物活性。
在实验室生物测定中，测试上述dsRNA分子对抗NCR的毒性。使用RNA处理的人工饲料法进行生物测定。简言之，将修改自Marrone等人1985(J.Econ.Entomol.[经济昆虫学杂志]78:290-293)的饲料的熔化的人工饲料倾倒进48孔板的每个孔中并允许凝固。将合成的dsRNA分子稀释至适当浓度，这样使得将20μl的溶液添加至48孔板的一半的孔中的饲料的表面，最终覆盖浓度为每孔0.5μg dsRNA。向每个孔中添加一个或两个NCR幼虫，以具有24至48个重复幼虫/测试dsRNA。将每个48孔板保持在约26℃和16:8的光照:黑暗光周期中。在侵染后第7天记录死亡率。将被设计成靶向GFP的dsRNA用作所有生物测定中的阴性对照，并且将被设计成靶向巴氏根萤叶甲(Dr)泛素基因的dsRNA用作阳性对照。
示于表5中的结果证明，被设计成靶向可从西方玉米根萤叶甲基因转录的mRNA的dsRNA分子也对巴氏根萤叶甲具有毒性。这表明本发明的靶标也是NCR的合适靶标，这样使得基于天然NCR mRNA的dsRNA分子也对NCR和其他根萤叶甲属具有毒性。
表5.处理后7天dsRNA对抗巴氏根萤叶甲的活性
实例6.WCR中的片段大小测定
使用引物设计工具Primer3自动设计在先前实例中测试的所有dsRNA样品，以基于每种靶基因的编码序列合成约500bp的dsRNA片段。如果编码序列的大小不允许500bp片段，则设计较小的片段。
在片段大小实验中，基于每种靶基因的完整编码序列设计不同的dsRNA片段。如果可获得并且如果不大于1000bp，则将完整编码序列作为整体进行测试。编码序列也被分成多个片段，在后续片段之间具有一些25bp-30bp的重叠。对于每个片段，设计新引物并在自动化文库合成平台上合成dsRNA。另外，也用不同的dsRNA片段测试靶BPA_16372，该靶BPA_16372的合成dsRNA分子被发现在单独的筛选中具有针对WCR的杀昆虫活性。在WCR生物测定中以两种不同浓度(0.1μg dsRNA/孔和0.01μg dsRNA/孔)测试所有dsRNA片段，并在第7天对死亡率进行评分。
在实验室生物测定中，测试上述dsRNA分子对抗西方玉米根萤叶甲的毒性。使用RNA处理的人工饲料法进行生物测定。简言之，将修改自Marrone等人1985(J.Econ.Entomol.[经济昆虫学杂志]78:290-293)的饲料的熔化的人工饲料倾倒进48孔板的每个孔中并允许凝固。将合成的dsRNA分子稀释至适当浓度，这样使得将20μl的溶液添加至48孔板的一半的孔中的饲料的表面，最终覆盖浓度为每孔0.1μg dsRNA或0.01μg dsRNA。向每个孔中添加一个或两个西方玉米根萤叶甲幼虫，以具有24至48个重复幼虫/测试dsRNA。将每个48孔板保持在约26℃和16:8的光照:黑暗光周期中。在侵染后第7天记录死亡率。将被设计成靶向GFP的dsRNA用作所有生物测定中的阴性对照，并且将被设计成靶向西方玉米根萤叶甲的泛素基因的dsRNA用作阳性对照。
示于表6中的结果证明，被设计成靶向可从西方玉米根萤叶甲基因转录的mRNA的dsRNA片段对西方玉米根萤叶甲具有毒性至高毒性。
表6.处理后7天dsRNA亚片段对抗西方玉米根萤叶甲的活性
实例7.dsRNA大小与对抗西方玉米根萤叶甲活性的相关性
这一实例描述了测试本发明的dsRNA对抗西方玉米根萤叶甲(WCR)的生物活性。
该实例聚焦于BPA_15366_5dsRNA的不同42bp-46bp亚片段(SEQ ID NO:313-317)。编码亚片段的DNA可操作地连接至89nt长的GFP序列，该序列充当“填充物”序列以产生133bp长的测试用序列(SEQ ID NO:318-322)。从1μg dsRNA/孔开始，以10倍稀释系列在实验室生物测定中测试SEQ ID NO:318-322中的每一个对抗WCR的毒性。使用RNA处理的人工饲料法进行生物测定。简言之，将修改自Marrone等人1985(J.Econ.Entomol.[经济昆虫学杂志]78:290-293)的饲料的熔化的人工饲料倾倒进48孔板的每个孔中并允许凝固。将合成的dsRNA分子稀释至适当浓度，这样使得将20μl的溶液添加至48孔板的一半的孔中的饲料的表面，最终覆盖浓度为每孔1μg、0.1μg、0.01μg和0.001μg。向每个孔中添加一个或两个WCR幼虫，以具有24至48个重复幼虫/dsRNA测试浓度。将每个48孔板保持在约26℃和16:8hr的光照:黑暗光周期中。在侵染后第1、2、3、4、5、6和7天记录死亡率。将被设计成靶向GFP的dsRNA用作阴性对照，并且将被设计成靶向WCR的肌钙蛋白基因的更大的133nt片段用作阳性对照。
示于表7中的结果证明，可操作地连接至“填充物”GFP序列(由SEQ ID NO:318-322编码)以产生133bp序列的五个42bp-46bp dsRNA片段几乎与BPA_15366_5(由SEQ ID NO:315编码)的133bp dsRNA片段一样有毒。第7天的死亡率％基于1μg dsRNA/孔浓度。在对GFPdsRNA的对照死亡率进行校正后，通过曲线拟合分析计算估计的LT50和LC50。LT50基于使用1μg dsRNA/天并以天数进行测量。LC50以μg dsRNA/孔进行测量。
表7.处理后7天dsRNA对抗西方玉米根萤叶甲的活性
实例8.鉴定具有对抗西方玉米根萤叶甲活性的21mer
这一实例描述了测试本发明的dsRNA对抗西方玉米根萤叶甲(WCR)的生物活性。
该实例聚焦于由BPA_15366_16(SEQ ID NO:321)编码的活性42bp dsRNA片段的不同21mer siRNA。将如上所述的亚片段嵌入GFP“填充物”序列中至总长为145bp。从1μgdsRNA/孔开始，以10倍稀释系列在实验室生物测定中测试嵌入的21mer中的每一个对抗WCR的毒性。使用RNA处理的人工饲料法进行生物测定。简言之，将修改自Marrone等人1985(J.Econ.Entomol.[经济昆虫学杂志]78:290-293)的饲料的熔化的人工饲料倾倒进48孔板的每个孔中并允许凝固。将合成的dsRNA分子稀释至适当浓度，这样使得将20μl的溶液添加至48孔板的一半的孔中的饲料的表面，最终覆盖浓度为每孔1μg、0.1μg、0.01μg和0.001μg。向每个孔中添加一个或两个WCR幼虫，以具有24至48个重复幼虫/dsRNA测试浓度。将每个48孔板保持在约26℃和16:8的光照:黑暗光周期中。在侵染后第1、2、3、4、5、6和7天记录死亡率。将被设计成靶向GFP的dsRNA用作阴性对照，并且将被设计成靶向BPA_15366_5(SEQ IDNO:287)的更大的133bp片段和嵌入GFP填充物序列(SEQ ID NO:321)的BPA_15366_16的42bp片段的dsRNA用作阳性对照。
示于表8中的结果证明，将被设计成靶向可从WCR BPA_15366靶基因转录的mRNA的大多数嵌入的21mer dsRNA片段显示出对抗WCR的毒性。
表8.处理后7天dsRNA对抗西方玉米根萤叶甲的活性
实例9.含有靶dsRNA的干扰RNA分子在玉米植物中的表达
该实例描述了将一种表达干扰RNA分子的构建体引入植物细胞。
载体构建
被设计成产生发夹RNA(hpRNA)的表达载体由含有启动子、正义链、用作环序列的内含子、反义链、以及终止子的盒组成。二元载体23130包含如下表达盒，该表达盒包含被设计成产生靶向BPA_15366(SEQ ID NO:403)的133个核苷酸的片段的hpRNA的DNA序列。在植物转化过程中，设计还含有在左边缘与右边缘之间的第二盒的每个二元载体以表达作为选择性标记的磷酸甘露糖异构酶(PMI)，该磷酸甘露糖异构酶提供代谢甘露糖的能力(美国专利号5,767,378和5,994,629，其通过引用并入本文)。这些载体还包含用于在细菌中进行选择的选择性标记。
农杆菌介导的转化
使用本领域的普通技术人员已知的标准分子生物学技术将含有发夹盒的生成的质粒转化进根癌土壤杆菌中。为了制备用于细胞转化的农杆菌，将农杆菌在28℃和220rpm下于液体YPC培养基中培养过夜。将以上描述的载体转化进玉米中。基本如描述于Negrotto等人,2000,Plant Cell Reports[植物细胞报告]19:798-803中的进行未成熟的玉蜀黍胚的农杆菌转化。对于这个实例，所有的培养基组分基本上如在以上Negrotto等人所说明。然而，本领域内已知的各种培养基成分可以被代替。转化、选择、和再生之后，测试植物的pmi基因和发夹dsRNA干扰RNA分子的存在。将来自PCR测定的阳性植物转移至温室中并允许结籽。
转基因玉米昆虫测定
在4个地点种植7个具有阳性和阴性对照的不同转基因事件的田间试验株。每个地点每个事件有三个重复区，并且每个区有12株植物。使用随机完全区组设计确定这些区的排列。田间试验依赖于玉米根虫的天然种群。当玉米植物达到R2早期阶段时，将每个区的5种植物的根挖出，洗净去除土壤，并使用0-3的节点损伤量表进行评级(Oleson等人,2005,J.Econ.Entomol.[经济昆虫学杂志]98:1-8)。使用最小二乘均值法(Least Square Means)和SAS JMP统计软件包分析根损害数据(JMPSAS机构2010)。结果示出在表9中。阳性对照植物包含事件5307(WO公开WO 2010/077816，通过引用并入本文)以及事件MIR604(WO公开WO2005/103301，通过引用并入本文)。“RDR”是评估的所有样品的1.5英寸根摄食等级的最小二乘均值。
表9：转基因玉米昆虫测定
植物ID RDR
阴性对照 2.12
阳性对照 0.66
事件1 0.61
事件2 1.33
事件3 0.82
事件4 0.80
事件5 1.29
事件6 1.50
事件7 1.37
如与非转基因对照植物相比，包含从来自载体23130的转基因表达的干扰RNA分子的所有事件对昆虫有害生物具有增强的耐受性。这说明如与非转基因对照植物相比，包含本发明的干扰RNA分子的转基因植物对昆虫有害生物具有增强的抗性。
实例10.用第二杀昆虫剂生物测定的干扰RNA分子
这个实例说明了与第二杀昆虫剂组合的本发明的干扰RNA分子的毒性。
针对BPA_15366靶标产生双链RNA分子。另外，制备第二杀昆虫剂。在实验室生物测定中，组合测试RNA和第二杀昆虫剂对抗WCR的毒性。
应当理解的是，在此描述的实例和实施例仅是出于说明性的目的，并且根据其说明书的不同修改或变化将提示本领域的技术人员并且将会包含在本申请以及随附的权利要求书的范围的精神和范围之内。
在本说明书中提到的所有公开物和专利申请对于本发明所涉及领域的技术人员的技术水平是指示性的。所有这些公开物和专利申请均通过引用结合在此，其程度如同每个单独的公开物或专利申请被确切地并单独地指明通过引用而被结合。
序列表
<110> Syngenta Participations, AG
Donohue, Kevin V.
Naudet, Yann
Feldmann, Pascale
Degrave, Lies
Maillet, Isabelle
<120> 使用RNA分子控制鞘翅目有害生物
<130> 81018-WO-REG-ORG-P-1
<150> 62/371259
<151> 2016-08-05
<160> 403
<170> PatentIn version 3.5
<210> 1
<211> 478
<212> DNA
<213> 玉米根萤叶甲属
<400> 1
ggtttcatga cccctgagag aaagaagaaa cttaggttac tgttgagaaa gaaagccgcc 60
gaagaattaa agaaagaaca agaacgcaaa gcagccgaaa ggaggcgtat cattgaagaa 120
aggtgcggta aacccaaact tgtcgatgac gcaaatgaag gcccattaaa acaagtatgt 180
gagggatatc acagacgtat tgtagaccta gaaaataaga aatttgacct cgaaaaagaa 240
gtggaattca gagattttca gatctccgaa ttgaacagcc aagtaaacga ccttagaggc 300
aaattcgtca aaccaacctt gaagaaggta tccaaatacg aaaacaaatt cgccaaactt 360
caaaagaagg cagctgaatt taacttccgt aaccaactca aagttgtcaa gaagaaagaa 420
ttcaccttag aagaagaaga caaagaaaag aaaccagact ggtcaaagaa gggagacg 478
<210> 2
<211> 529
<212> DNA
<213> 玉米根萤叶甲属
<400> 2
agaagttgcc gctttagtcg tagacaatgg atccggtatg tgcaaagctg gttttgctgg 60
ggatgatgca cctcgtgctg tattcccttc aattgttgga cgcccaagac atcagggtgt 120
gatggtagga atgggacaaa aagattccta tgtaggtgat gaagctcaaa gtaaaagagg 180
tatccttacc ttaaaatacc ccatcgagca cggaatagtc acaaactggg atgatatgga 240
gaaaatttgg catcatacat tctacaatga actcagagta gccccagaag aacaccctgt 300
tctgttgaca gaagctcctc tcaaccccaa ggccaacagg gaaaagatga cacaaataat 360
gtttgaaact ttcaacaccc cagccatgta tgttgccatc caggctgtac tctccttgta 420
tgcatctggt cgtacaactg gtattgtgtt ggattctggt gatggtgtat cccacactgt 480
cccaatctat gaaggttatg ctcttcctca tgcaatcctt cgtttggac 529
<210> 3
<211> 592
<212> DNA
<213> 玉米根萤叶甲属
<400> 3
accggccttt gtatgtcttg ggatatgtgc ctaaagacga tagattatac ctcgtagata 60
aagagttgcg cgtagtaagc taccaattac ttctttctgt tcttgaatat caaactgccg 120
tcatgagaag agactttcca acagcagaca gagtacttcc gtccattcct aaggagcaca 180
gaacgagagt ggcacatttc ttagaaaagc aaggcttcaa acagcaagct ttggccgtaa 240
gtacagatcc agagcacaga ttcgagctgg cagtagcatt agaggatctt aatatagcca 300
aaactctagc tcaagaagcg aacagtccgc aaaagtggaa tcaactagca gaattggcag 360
ctgctactaa taatgtaagc gtagccaagg aatgtatgca aaaagcgcaa gattatggag 420
gcttgttgct tcttgctacg agctccggtg atgaaaattt agtccgtact ctaggagaaa 480
cgacacaagc tgaaagcaaa cataacttag catttttgtc acacttgtta gtaggtgatt 540
taaacaaatg tctagacatt cttattaata ccggtagatt gccagaagct gc 592
<210> 4
<211> 594
<212> DNA
<213> 玉米根萤叶甲属
<400> 4
gaaagcagtt ggaagatggc cgtactctct cagactacaa cattcaaaaa gagtctaccc 60
tccatttggt acttcgtctt agaggaggta tgcagatttt tgttaaaact ttaactggaa 120
agaccatcac ccttgaagta gaaccttctg ataccatcga aaatgtcaaa gccaaaattc 180
aagacaaaga aggtattcca ccagatcaac aaagattaat ctttgccgga aagcaattgg 240
aagatggtcg tacactctca gactacaaca ttcaaaagga atctaccctc catttggtac 300
ttcgtcttag aggaggtatg caaatctttg taaaaacact cactggtaag accatcaccc 360
tcgaggttga accatcagat accatcgaga atgtcaaagc taaaattcaa gacaaagaag 420
gtattccacc agatcaacag agattaatct tcgctggaaa gcagttggaa gatggccgta 480
ctctctcaga ctacaatatt cagaaagagt ctaccctcca tttggtactt cgtcttagag 540
gaggtatgca aatctttgta aaaactctca ctggtaagac catcaccctc gagg 594
<210> 5
<211> 547
<212> DNA
<213> 玉米根萤叶甲属
<400> 5
taccccattg aacacggaat tatcactaac tgggacgata tggaaaagat ctggcatcac 60
accttctaca atgaacttag agtagccccc gaagaacatc ccattctttt gactgaagct 120
ccacttaacc caaaagccaa cagagaaaag atgactcaaa tcatgtttga aactttcaat 180
acccctgcca tgtatgttgc cattcaagct gtattgtctc tgtacgcttc cggtcgtacc 240
actggtattg tacttgattc tggagatggt gtatcccaca cagtacccat ctatgaaggt 300
tacgctctcc cacacgccat cttgcgtttg gacttggccg gtagagactt gactgactac 360
cttatgaaga tcttaaccga aagaggttac tctttcacca ccacagctga aagagaaata 420
gttcgtgaca tcaaggaaaa attgtgctat gtagctttgg acttcgaaca ggaaatggcc 480
acagcagcca gctccacctc cttagaaaag agttatgaac ttcctgacgg tcaagtcatc 540
accattg 547
<210> 6
<211> 592
<212> DNA
<213> 玉米根萤叶甲属
<400> 6
aggaaaggaa ggacccaaga ccaaacaacc atcgagatac atccgtttta tctacaatcg 60
cgtcatattg gaatgtcctt ctgtaagagc tgctgcagtc tccgccatgg cacaattcgg 120
agcctcttgt cccgatttgt tagaaaatat ccaaatatta ctttcgaggt gtcagatgga 180
ttcagacgat gaagttaggg acagagctac atattatagt aatatactta acaaaaatga 240
taaaagttta tacaacaatt acattttgga ttctttgcag gtttcaattc cttcactaga 300
aagatcgctt agagaataca ttcaaaatcc aactgacgaa ccatttgaca ttaagtccgt 360
acctgtagca gcagtgccaa cagcagaaga acgagaagtt aaaaacaaat ctgaaggact 420
gctagtctct caaggtccag tccgacctcc tccggtgtct agagaagaaa acttcgccga 480
aaaacttagt aacgttccgg gtatacaaca gttaggacct ttgttcaaaa cttccgacgt 540
cgttgaactc actgaatctg aaacagagta ttttgtccgc tgtatcaagc ac 592
<210> 7
<211> 500
<212> DNA
<213> 玉米根萤叶甲属
<400> 7
tactaggcga aagtgccgtc ggtaagtcga gtttggtact gaggttcgtc aaaggacagt 60
tccacgaata ccaggagagt accataggag cagctttcct tacacaaacc atatgcctcg 120
acgatacaac tgttaaattt gaaatttggg acacagcggg tcaagaaagg taccacagtt 180
tagctcctat gtactatagg ggcgcacagg cagctatagt cgtctacgac ataaccaatc 240
aagacacatt cggcagggcg aaaacgtggg tgaaggaact tcaaaggcag gccagtccga 300
cgatcgtgat agctttggcc ggcaacaagc aagatttggc caacaaacgt atggtagaat 360
acgaagaggc gcagacgtat gctgacgaaa acggcttact ttttatggaa acttccgcaa 420
agacggcaat gaacgtcaac gatatatttt tagcaatagc taagaaactg cccaagaatg 480
aacaaaccac aggtcaaggc 500
<210> 8
<211> 189
<212> DNA
<213> 玉米根萤叶甲属
<400> 8
cgatgaggtt gaaggaagga gaaaaatttt gatggggcga aaaagcatta ccaggacata 60
tcttcgtgga aatgctgttc ctgcgtatgt gataataatc cttgtaggaa ttggtcaaat 120
catcctggga gggatattgt acgttgcatt gaggaagaag atcattgctg cacctgtaac 180
ggcatcata 189
<210> 9
<211> 393
<212> DNA
<213> 玉米根萤叶甲属
<400> 9
tgaggtcgag ccctcagata ctatcgaaaa tgtgaaagct aaaatccagg ataaagaagg 60
aattccccca gaccagcaac gtctcatctt cgctggaaaa caactcgaag atggtcgtac 120
cttgtctgac tataatattc aaaaagaatc aacccttcac ttggtgttga gattgagagg 180
aggtgctaag aaacgtaaga agaagaatta ctccaccccc aagaaaatca agcacaagaa 240
gaagaaggtt aagttagctg tattgaaatt ttataaggtt gacgaaaatg gtaaaatcca 300
ccgattgaga cgtgaatgcc ccgctgaaca atgtggagct ggtgtcttca tggcagccat 360
ggaagacagg cattactgtg gcaagtgcgg tta 393
<210> 10
<211> 524
<212> DNA
<213> 玉米根萤叶甲属
<400> 10
tctttgcctt tggctacgat tcgaggaggg cattcaagaa tggctccaga aattggattc 60
agaacaattt tacatcggag tatatgtact tatagtcgct tcactgatcg tcatgattgt 120
gtcctttata ggatgtatta gtgccctgca ggagagtacc atggcccttt tagtgtacat 180
cggcacccaa gtgctcagtt ttatattcgg tttatccggt tcggcggttc ttctggataa 240
cagcgccaga gattcccact tccaaccgag gatccgagag agtatgcgac gtcttatcat 300
gaatgctcat cacgaccaat ccagacaaac actagccatg attcaggaaa atgttggttg 360
ctgcggagct gatggcgcaa cagactacct ctctcttcag cagccccttc caagtcagtg 420
cagagacacc gttactggaa acccattctt ccacggatgt gtagatgaac tcacctggtt 480
cttcgaagaa aaatgtggtt ggatagcagg tttagctatg gcga 524
<210> 11
<211> 267
<212> DNA
<213> 玉米根萤叶甲属
<400> 11
tcgtgctggt ttacaatttc ctgtaggtcg tattcatcgt ttattgagaa aaggaaatta 60
tgccgaaaga gttggtgctg gagctcctgt atacttggca gctgttatgg aatatttagc 120
tgctgaagtt ttggaattgg caggaaatgc agctagagat aacaaaaaga cccgtataat 180
tcctagacat ttacaattgg ccataagaaa tgacgaggaa ttgaacaaat tactgtcagg 240
agttaccatc gcccaaggtg gagtatt 267
<210> 12
<211> 558
<212> DNA
<213> 玉米根萤叶甲属
<400> 12
gtgcatgaag ttggatggtg tagatctgcc cccaccaatt agcttcgaca ttgcggaaga 60
gcaaccgtta ccaccttgcc aacagacgtt cttatgtaat ggtgatggag gatccatagt 120
gcgacagttt ctcgagctgt atttcgtaat atatgattca gataataggc agtcccttct 180
tcaggcatat cacgaaaaag ccacattttc aatgacaatg gcctacccgt acggctattc 240
caaagacagt aaaggagtat cgtggttgaa ttggtatgcc accgataata gaaatttatt 300
acgagttcaa gatccagaca gaagaaacaa gttgttaaga cagggacaag ttgctgtagt 360
ttcgttcttg caagatatgc cgcacacgaa gcacgatatt cacagtttta cagtagattt 420
gacagttttt acaccccaga tgttatgttt gacagtggct ggtatgttta aagaattgaa 480
aagtggccac aaagtacctc ctttaagata tttcttcaga acccttgtaa ttgtacctgc 540
tggatcaggt ttttgcat 558
<210> 13
<211> 582
<212> DNA
<213> 玉米根萤叶甲属
<400> 13
gattcggagc cctacaatga aatgcaacta aaaatggatt tagaaaaggg tgaggttaaa 60
gtaaaaataa gagcattaga aaaaataatt cacatgattc tggcaggaga aaggttgccg 120
aatggatttc taatgaccat cataagaaac gttttacctt tacaagatca tttggcaaaa 180
aaactattat tgattttctg ggaaatagtt ccaaaaacaa atccagaagg taaactacta 240
caagagatga ttttggtatg tgatgcctat agaaaagatc tgcaacaccc aaatgaattt 300
ttgagaggtt ctacacttcg cttcttgtgc aaactgaagg aaccagaatt gttggaacca 360
ttaatgccca gtattagagc ttgtttggat cataggcaca gctatgtgag gaggaatgct 420
gtactggcaa tttttaccat ttacaaaaat tttgaagccc tcattccaga tgctcctgaa 480
ctgatctcca attatttgga tggtgagcaa gacatgtctt gtaaaagaaa tgcgttttta 540
atgcttcttc atgctgacca agaaagggcg ttgtcgtatt tg 582
<210> 14
<211> 458
<212> DNA
<213> 玉米根萤叶甲属
<400> 14
ttggcaaaaa ggaaatgagg atattgatgg taggactcga tgcagctggt aaaaccacaa 60
ttttatataa acttaaatta ggagaaattg taacaactat tccaacaatt ggatttaatg 120
tggagactgt agaatataag aacattagtt ttacagtatg ggatgtaggt ggtcaagata 180
aaattaggcc attgtggaga cactatttcc aaaacacaca aggcctaatt ttcgtagtag 240
acagtaacga cagggaacgt atcactgagg ctaaagatga attaatgcgt atgttggccg 300
aagatgaact tagagatgcc gtacttctca ttttcgccaa caaacaagat ttgcccaatg 360
caatgaacgc tgcagaaatc accgacaaac tcggtctcca ttcactacgc aaccgcaact 420
ggtacattca agctacctgt gcaactagcg gagatggt 458
<210> 15
<211> 592
<212> DNA
<213> 玉米根萤叶甲属
<400> 15
ggtcgcgaaa gaacagaaag attttttgac cgtatctgcc gatggatgcg tacaagtaac 60
aaaacctctc gaccgagatc cgcctttcgg tagcccaaca cgacaagtct tcatctatgc 120
tcgtgataat gatggaggca caaattcatt gttggccact gcagaaattg aaattatttt 180
aatagatata aacgataatg ctcccttttt aaatgttaca gaaattgttt attatgaaaa 240
ccaggatcca ggttttatag gtaacctaag tgccgatgat tacgatggtc ctgataatgg 300
acctccgttt gcttttcgat tatcagacac tgcttcagat agtattagat cgaaattttc 360
cattatcgga aaccagcttt tcgctttaga aatgtttgat agagaagagc aaaaatatta 420
tgacattgcc attgacatta cagatagtgg agtacctcca ctaacaggaa ctagtattct 480
tagagttata atcggagatg taaatgataa tccagctaca gacggaaaca gcacgatctt 540
tgtgtataag tacgtcaatg ggccagaaaa tttcatggaa atcggacgtg ta 592
<210> 16
<211> 214
<212> DNA
<213> 玉米根萤叶甲属
<400> 16
gcatggggta tcatccagtt gggtttcatg ggtgtattct attacattgg ggctgtggct 60
ttagcagaag atattccaga ggttgagttt aagggcgatt tagacaaatt ttatagcgac 120
gtcaacacgg gtttcacaca gaatgcttac aactgctgga ttgctgctct cctatacctg 180
ataacattag cagtatcagc tcaccaattc tggg 214
<210> 17
<211> 459
<212> DNA
<213> 玉米根萤叶甲属
<400> 17
tagatgccgc aggtaaaacc acaatcttat acaaattgaa gcttggtgaa atcgtaacta 60
caataccaac catcggcttc aatgtagaaa ccgttgagta caagaatata tctttcacgg 120
tatgggatgt aggtggccag acgagaatca gaaaactctg gagacactat ttcgccaaca 180
ctgatggact catttttgtg gttgattcca acgaccgaga ccgtatcgcg gaagccgaag 240
aagaattgca caatatgtta ggagaggacg atttaagaga ctgcattttg ttaatattcg 300
ccaacaaaca agatttaccg aactcgatgt ccactgctga attgaccgat aagcttaagt 360
tgcacacttt gaagaatagg aggtggtaca tacaagccac atgtgctact caagggaatg 420
gtttgtacga aggactagat tggttgtcga atgaattgg 459
<210> 18
<211> 536
<212> DNA
<213> 玉米根萤叶甲属
<400> 18
gctaccctag cgtccactgt aacactgatt tttgccctct actactgcct cacgggaaaa 60
ggagagcaag ttagtttagc atggttattg ttgaatgtgt ctccccacat gtgggcaggt 120
ctaggaattg gccttgctgt atcattatca gttgtaggag ctgctgcagg aattcacact 180
acaggagtca gtatcgtagg agctggtgtt aaagccccca gaatcaaaac caaaaattta 240
atttctatta ttttctgtga agctgtggct atctatgggt taattatggc tatagtactc 300
tgtggaagtt ggaagaattt cgatgtagac ctattcaacc tcaaaactca taactttgct 360
caaaaccatt atggatcaca tgttattttt ggatccggtt taactgttgg atttgtaaat 420
ctattatgtg gattttgtgt tggagtagtt ggttctggtg cagccatttc tgatgcagcc 480
aattcatcat tattcgtcaa aattttgatt attgagattt ttggaagtgc cattgg 536
<210> 19
<211> 547
<212> DNA
<213> 玉米根萤叶甲属
<400> 19
accttatggg aaagcgtgtg gacttttctg cacgtactgt catcacacca gatcccaatt 60
tacgtatcga ccaagtagga gtgcctagaa gtattgctca aaacatgacg tttccagaaa 120
tcgtcacacc tttcaatttt gacaaaatgt tggaattggt acagagaggt aattctcagt 180
atccaggagc taagtatatc atcagagaca atggagagag gattgattta cgtttccacc 240
caaaaccgtc agatttacat ttgcagtgtg gttataaggt agaaagacac atcagagacg 300
gcgatctagt aatcttcaac cgtcaaccaa ccctccacaa gatgagtatg atgggccaca 360
gagtcaaagt cttaccctgg tcgacgttcc gtatgaatct ctcgtgcacc tctccctaca 420
acgccgattt tgacggcgac gaaatgaacc tccatgtgcc ccaaagtatg gaaactcgag 480
ctgaagtcga aaacctccac atcactccca ggcaaatcat tactccgcaa gctaaccaac 540
ccgtcat 547
<210> 20
<211> 564
<212> DNA
<213> 玉米根萤叶甲属
<400> 20
cggatctcta tttgggggat caagtcgtat tgaagatgca gtggaatgtt acacaagagc 60
tgcaaacctt tttaaaatgg ccaagagctg ggatgctgcc ggtaaagcct tttgtgaggc 120
tgctaatttg cattccagaa ctggtgctcg tcatgacgct gccactaatt atatagatgc 180
tgcaaattgt tacaaaaaag ccgatgtatt tgaggctgta aactgcttta taaaagctat 240
agacatttat accgaaatgg gtcgctttac aatggctgca aaacaccatc agactattgc 300
agaaatgtat gagactgatg ctgtggacat cgaaagggct gttcaacact atgaacaggc 360
ggctgattac ttcagaggag aagaaagcaa tgcttccgcc aataagtgtc ttcttaaagt 420
ggctcaatat gcagcccaac ttgaaaacta tgaaaaagca gtgggaattt atcaagaagt 480
ggcttatgca gctctggaaa gctctctttt aaaatacagt gcaaaggaat acttattcag 540
agctgccctt tgtcaccttt gtgt 564
<210> 21
<211> 564
<212> DNA
<213> 玉米根萤叶甲属
<400> 21
tgctgattgg agattcagga gtaggaaaat cttgtcttct actgagattt gcagatgata 60
cctacacaga aagctatatt agtaccattg gcgtagattt taaaatcagg acaatcgatt 120
tagatggaaa gacaattaaa ttgcaaattt gggatacagc aggtcaggaa aggtttagaa 180
cgattacatc aagttattac cgaggagcac atggtattat tgtagtgtac gattgcacag 240
accaagattc attcaataac gttaaacagt ggctcgaaga aatcgaccgt tatgcgtgtg 300
acaatgtaaa caaattactg gtagggaata aaagcgattt gacaactaag aaagttgtcg 360
acttcactac agccaaggag tatgccgacc aattgggtat accatttttg gaaacctcag 420
ctaagaatgc aaccaatgta gaacaggcct ttatgactat ggccgctgaa ataaaaaata 480
gagtaggacc tccatcttct gcggtagacc aaggaaataa ggttaggttc gatcaaagtc 540
gcccagtcga aacaaccaaa tccg 564
<210> 22
<211> 502
<212> DNA
<213> 玉米根萤叶甲属
<400> 22
gagctatagt ggactgcggg ttcgaacatc cttcagaagt tcaacatgaa tgtattcctc 60
aagctgtcat tggcatggat attctgtgcc aagctaaatc cggtatggga aaaacggctg 120
tttttgtatt agctacactc caagtaatag atcctacaga aaatgttgta tatgttctcg 180
tcatgtgcca taccagagag ttagccttcc agataagcaa agagtacgaa cgtttcagta 240
aatatatgcc caatattaaa gtaggggtct tctttggtgg cttgcctatc cagaaagatg 300
aggaaacgtt aaaaaataat tgcccgcata tcgttgtggg tactccagga agaattttag 360
cattggtcag atcgaaaaaa cttaatctca aacatctaaa gcattttatt ttggatgaat 420
gtgataaaat gttggagtta ttagacatga gacgtgatgt tcaagaaata tatcgtaaca 480
ctccccacga aaaacaagtc at 502
<210> 23
<211> 505
<212> DNA
<213> 玉米根萤叶甲属
<400> 23
tggtagattt ggctaacctc gtatattggt gtttaggtct taatattccg tacgttagtt 60
tctatgatta taaaggtaat ttaaaaaagc atgaagagaa gttgcaacaa attgtagaat 120
ccagaaaatc agagaatatc aacataattt ggcacaccca tgcagaacaa aggcataaaa 180
atggattttt gggtccaaaa atccacgtaa aagtgttaac acacgcggac ggaaagcaaa 240
gtatagtaaa tgttactaaa aaattagctc taaataaaga aaaagacatt agtaaagaaa 300
aaattagtga attactatta aggcagtatg aatttccaga tccagaaatg gctattattt 360
gtggaaagaa actgaacatt tataattatc ctccttggca gttaagactc acagaattct 420
ttaaagtcaa caaagtcaac aacatcacat tcccagtgtt tgtggaaaaa ttggaaaagt 480
acagcaaatg tgaacagagg gtggg 505
<210> 24
<211> 410
<212> DNA
<213> 玉米根萤叶甲属
<400> 24
aaccagggat ctatttgccc gcaagtttaa aaaacgtggt gtaattccac tttccacata 60
tttgagagtc tacaaagttg gagatattgt agatatcaag ggtaatggtg cagttcaaaa 120
gggtatgccc cacaaagtgt accatggtaa gacaggacgt gttttcaatg ttactgcaca 180
tgcattaggt gtaattgtaa acaaaagggt tcgaggaaga atcatcccca aaagaatcaa 240
tctccgtatt gaacatgtaa accactccaa gtgtcgtcaa gacttcttgc aaagagtaaa 300
atccaacgaa aagctacgta aagaagctaa agaaaagaac attaaagtag aacttaggag 360
acaacctgcc caacctaggc cagcacatat tgttagcgga aaggttccag 410
<210> 25
<211> 467
<212> DNA
<213> 玉米根萤叶甲属
<400> 25
tgatggtaat agagtgctgg ctaaatacta cgataaagat atatttccta cagcaaaaga 60
gcagaaagct tttgagaaaa atttgttcaa taaaactcat agggcagacg cagaaattat 120
catgttggat ggtttaactt gtgtgtatag aagtaatgta gatttattct tttatgttat 180
gggcagttca catgaaaatg agctaatttt aatgagtgtt ttaaattgct tgtatgactc 240
agtaagtcaa atattgaaga aaaatatgca aaaacgagct gtcttggaat cactagatat 300
tgttatgctg gctatggatg aaattgttga tggaggaata attatagatt ctgattcaag 360
ttcagtagta tctagaatag cattaaggac tgatgatatt ccattaggag aacaaactgt 420
agctcaggta ttccaaacgg ccaaagaaca gctgaaatgg tcattgc 467
<210> 26
<211> 368
<212> DNA
<213> 玉米根萤叶甲属
<400> 26
caccgaagaa caaattgctg aattcaaaga agctttctca ctattcgata aagatggtga 60
tggtacaatt acgactaaag aattaggaac agtaatgaga tctctaggac aaaatccaac 120
agaggctgaa ttacaggata tgatcaatga agtagatgcc gatggtaacg gcacgatcga 180
tttcccagaa tttttaacga tgatggcacg taaaatgaaa gataccgata gtgaggaaga 240
aattcgtgaa gcattccgag tgttcgacaa agacggcaat ggtttcatct cagcagcaga 300
attgcgccac gtcatgacca acttgggtga aaaattgaca gacgaagaag tcgatgaaat 360
gattcggg 368
<210> 27
<211> 430
<212> DNA
<213> 玉米根萤叶甲属
<400> 27
ttcgcacaag atgactttgg tggtgaaaat gttgatctat atgacgatgt aatatccgct 60
cctcctggaa ataatgacaa cccaggtgat tcaaatcatc atgctcctcc tggtgctggt 120
gaagatggtg gaggtaattt tgttgggtca ggaggagcac ccaataatat aaattcttct 180
ggaagaagac atcagctgta tgttggaaat ctgacttggt ggacaactga tcaagatata 240
gaaaatgcag tgcatgatat aggggtaacc gacttccatg aagttaagtt ttttgaacac 300
agagcaaatg gtcaatccaa gggattctgt gtcatatctt tgggatctga gggaagcatg 360
agactctgcc tggaactcct atctaaaaaa gagatcaatg gccaaaatcc ccttgttacc 420
cttcccacaa 430
<210> 28
<211> 505
<212> DNA
<213> 玉米根萤叶甲属
<400> 28
taggaatggc attttcaggc ttaatagctg atgcaaggca aatcgttgag attgctagaa 60
aagaagcatc aaattataga catcaatatg gttcaaatat tcctcttaaa tacctaaatg 120
atagagtaag catgtacatg catgcataca ctttatacag tgctgttaga ccatttggtt 180
gcagtgtcat cttggccagt tatgaagata gtgacccatc tatgtatctg attgatccat 240
ctggagttag ctatggatac tttggatgtg ctacaggtaa agcaaaacag tctgcaaaga 300
ctgaaataga aaaattgaag atggggaatc taacatgcaa agaacttgtt aaagaagcag 360
ccaaaatcat ttatttggtc catgatgagc tgaaggataa gaattttgaa ctggaacttt 420
catgggtatg caaagatacg aatggtttac ataccaaagt gcctgaatca gtgtttgctg 480
atgcagaaaa agctgccaaa caagc 505
<210> 29
<211> 548
<212> DNA
<213> 玉米根萤叶甲属
<400> 29
tccatctaga ggagcccaaa tgatgatgaa atccaggcta aagggagccc aaaagggaca 60
tagtttatta aagaagaaag ctgatgcttt acaaatgaga tttagaatga ttttgaacaa 120
aattattgag accaaaactc tcatgggtga agtaatgaaa gaagctgcct tttctttagc 180
tgaagcaaag tttgcaactg gtgacttcaa tcaagttgtt cttcaaaatg tcaccaaggc 240
tcaaataaaa ataagaacta agaaagacaa cgttgctggt gttactttac cagtgtttga 300
atgctaccaa gatggtacag atacatatga gttggctggt ttggctaggg gaggtcaaca 360
attgacaaaa ctcaagaaga attatcaaag tgctgttaaa ctgttggttg aattagcctc 420
tttgcaaact tcttttgtaa ctcttgatga tgtaatcaaa ataacaaaca gaagagtcaa 480
tgccattgaa catgttatca ttccaagaat agagcgtact ttggcttaca tcatatccga 540
actggacg 548
<210> 30
<211> 521
<212> DNA
<213> 玉米根萤叶甲属
<400> 30
gatctggaag cgctagttgc aaaagtagac gaaatgagaa cccaaagagc catgctatgg 60
gctcaacttc gagaatctat tcaccaagac gatattacaa gttcccttgt aacgaaacaa 120
ccaaatcagt cgctggaaca gctgttccag caagaacttc aaaagcatca aaatctgatt 180
tcgttgattg aacaaaacac ctcggcacaa gaaaacatta agagcgcctt agtcgattct 240
tacgcttacg ctgtaaattc aagaaaatac atccaagata tactccaaaa gagaaccaca 300
accataacgt cactgatagc atcgttcgac tcttacgaag acttattggc aaaagctaac 360
aaagggatag agttttactc aaaacttgaa acgaacgtat ccaagttact gcaaagaata 420
aggagtacct gcaaagttca acaagaagag cgagatcaga tgatgtcgac tgcgcaagtg 480
cctcaatggg agagtcatac gtcacttgcc gctcctaaac t 521
<210> 31
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 31
ggtttcatga cccctgagag 20
<210> 32
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 32
cgtctccctt ctttgaccag 20
<210> 33
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 33
agaagttgcc gctttagtcg 20
<210> 34
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 34
gtccaaacga aggattgcat 20
<210> 35
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 35
accggccttt gtatgtcttg 20
<210> 36
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 36
gcagcttctg gcaatctacc 20
<210> 37
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 37
gaaagcagtt ggaagatggc 20
<210> 38
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 38
cctcgagggt gatggtctta 20
<210> 39
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 39
taccccattg aacacggaat 20
<210> 40
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 40
caatggtgat gacttgaccg 20
<210> 41
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 41
aggaaaggaa ggacccaaga 20
<210> 42
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 42
gtgcttgata cagcggacaa 20
<210> 43
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 43
tactaggcga aagtgccgtc 20
<210> 44
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 44
gccttgacct gtggtttgtt 20
<210> 45
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 45
cgatgaggtt gaaggaagga 20
<210> 46
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 46
tatgatgccg ttacaggtgc 20
<210> 47
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 47
tgaggtcgag ccctcagata 20
<210> 48
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 48
taaccgcact tgccacagta 20
<210> 49
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 49
tctttgcctt tggctacgat 20
<210> 50
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 50
tcgccatagc taaacctgct 20
<210> 51
<211> 21
<212> DNA
<213> 玉米根萤叶甲属
<400> 51
tcgtgctggt ttacaatttc c 21
<210> 52
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 52
aatactccac cttgggcgat 20
<210> 53
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 53
gtgcatgaag ttggatggtg 20
<210> 54
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 54
atgcaaaaac ctgatccagc 20
<210> 55
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 55
gattcggagc cctacaatga 20
<210> 56
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 56
caaatacgac aacgcccttt 20
<210> 57
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 57
ttggcaaaaa ggaaatgagg 20
<210> 58
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 58
accatctccg ctagttgcac 20
<210> 59
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 59
ggtcgcgaaa gaacagaaag 20
<210> 60
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 60
tacacgtccg atttccatga 20
<210> 61
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 61
gcatggggta tcatccagtt 20
<210> 62
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 62
cccagaattg gtgagctgat 20
<210> 63
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 63
tagatgccgc aggtaaaacc 20
<210> 64
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 64
ccaattcatt cgacaaccaa 20
<210> 65
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 65
gctaccctag cgtccactgt 20
<210> 66
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 66
ccaatggcac ttccaaaaat 20
<210> 67
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 67
accttatggg aaagcgtgtg 20
<210> 68
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 68
atgacgggtt ggttagcttg 20
<210> 69
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 69
cggatctcta tttgggggat 20
<210> 70
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 70
acacaaaggt gacaaagggc 20
<210> 71
<211> 21
<212> DNA
<213> 玉米根萤叶甲属
<400> 71
tgctgattgg agattcagga g 21
<210> 72
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 72
cggatttggt tgtttcgact 20
<210> 73
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 73
gagctatagt ggactgcggg 20
<210> 74
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 74
atgacttgtt tttcgtgggg 20
<210> 75
<211> 21
<212> DNA
<213> 玉米根萤叶甲属
<400> 75
tggtagattt ggctaacctc g 21
<210> 76
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 76
cccaccctct gttcacattt 20
<210> 77
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 77
aaccagggat ctatttgccc 20
<210> 78
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 78
ctggaacctt tccgctaaca 20
<210> 79
<211> 22
<212> DNA
<213> 玉米根萤叶甲属
<400> 79
tgatggtaat agagtgctgg ct 22
<210> 80
<211> 21
<212> DNA
<213> 玉米根萤叶甲属
<400> 80
gcaatgacca tttcagctgt t 21
<210> 81
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 81
caccgaagaa caaattgctg 20
<210> 82
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 82
cccgaatcat ttcatcgact 20
<210> 83
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 83
ttcgcacaag atgactttgg 20
<210> 84
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 84
ttgtgggaag ggtaacaagg 20
<210> 85
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 85
taggaatggc attttcaggc 20
<210> 86
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 86
gcttgtttgg cagctttttc 20
<210> 87
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 87
tccatctaga ggagcccaaa 20
<210> 88
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 88
cgtccagttc ggatatgatg 20
<210> 89
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 89
gatctggaag cgctagttgc 20
<210> 90
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 90
agtttaggag cggcaagtga 20
<210> 91
<211> 633
<212> DNA
<213> 玉米根萤叶甲属
<400> 91
atggcggacg atgagagaaa gaaactggag gaggaaaaga agaggaaaca ggccgaaatt 60
gaacgcaaaa gggccgaggt cagggctcgt atggaagagg cctcaaaagc caagaaggcc 120
aagaaaggtt tcatgacccc tgagagaaag aagaaactta ggttactgtt gagaaagaaa 180
gccgccgaag aattaaagaa agaacaagaa cgcaaagcag ccgaaaggag gcgtatcatt 240
gaagaaaggt gcggtaaacc caaacttgtc gatgacgcaa atgaaggccc attaaaacaa 300
gtatgtgagg gatatcacag acgtattgta gacctagaaa ataagaaatt tgacctcgaa 360
aaagaagtgg aattcagaga ttttcagatc tccgaattga acagccaagt aaacgacctt 420
agaggcaaat tcgtcaaacc aaccttgaag aaggtatcca aatacgaaaa caaattcgcc 480
aaacttcaaa agaaggcagc tgaatttaac ttccgtaacc aactcaaagt tgtcaagaag 540
aaagaattca ccttagaaga agaagacaaa gaaaagaaac cagactggtc aaagaaggga 600
gacgaaaaga aggtacaaga ggctgaagca tga 633
<210> 92
<211> 603
<212> DNA
<213> 玉米根萤叶甲属
<400> 92
atgtgtgaag aagaagttgc cgctttagtc gtagacaatg gatccggtat gtgcaaagct 60
ggttttgctg gggatgatgc acctcgtgct gtattccctt caattgttgg acgcccaaga 120
catcagggtg tgatggtagg aatgggacaa aaagattcct atgtaggtga tgaagctcaa 180
agtaaaagag gtatccttac cttaaaatac cccatcgagc acggaatagt cacaaactgg 240
gatgatatgg agaaaatttg gcatcataca ttctacaatg aactcagagt agccccagaa 300
gaacaccctg ttctgttgac agaagctcct ctcaacccca aggccaacag ggaaaagatg 360
acacaaataa tgtttgaaac tttcaacacc ccagccatgt atgttgccat ccaggctgta 420
ctctccttgt atgcatctgg tcgtacaact ggtattgtgt tggattctgg tgatggtgta 480
tcccacactg tcccaatcta tgaaggttat gctcttcctc atgcaatcct tcgtttggac 540
ttagctggta gagacttgac tgattacctc atgaaaattt tgactgaacg tggctactct 600
ttc 603
<210> 93
<211> 2742
<212> DNA
<213> 玉米根萤叶甲属
<400> 93
atgccacttc gattagatat aaaaagaaag ctaacagctc gctcagaccg ggtaaaatgt 60
gtggatcttc accctacaga accttggatg ctgtgttctc tttacagcgg aaatataaac 120
gtttggaaca ccgaaaatca gcaactggtt aagacttttg aagtatgtga tgtacctgtt 180
cggacagcta agtttttgcc caggaagaac tggatagtca gtgggtctga tgatatgcag 240
attcgagttt tcaattacaa taccttagat cgggtacatt cttttgaggc tcattcggat 300
tatgtgagat gtattgtcgt acaccctaca caaccttata tattaacaag tagtgatgat 360
atgcttatca agctttggaa ttgggaaaaa gcatgggctt gtcagcaagt tttcgaagga 420
cacactcatt atattatgca aatcgccata aatccaaaag acaacaacac atttgccagt 480
gcatccctag atagaacatt gaaagtatgg caattgggag cgtccacagc gaatttcaca 540
ctagaaggtc atgagaaagg cgttaactgt gtggactatt atcacggtgg agataaacct 600
tatttaatct caggcgctga tgatagatta gtaaaaatct gggattatca aaacaaaact 660
tgtgttcaaa ctttggaagg acatgctcaa aatgtaaccg ctgcatgttt ccatccagaa 720
cttcctgtag ctcttactgg aagtgaagat ggtactgtca gagtgtggca tgccaacacc 780
cataggttag aaagtagctt aaattatggc tttgaaagag tatggactat tttctgccta 840
aagggatcca ataacgtggc attgggttat gatgaaggta gcattttggt taaagttggt 900
agagaagaac cagctgttag tatggatgcc agtggaggca aaattatttg ggccagacac 960
tctgaacttc aacaggcaaa tctcaaggcg ttagctgaag gtgcggaaat aagagatgga 1020
gaacgccttc cagtttctgt aaaagatatg ggtgcttgcg agatataccc tcagacaatt 1080
caacacaatc ccaatggccg ttttgttgtt gtctgtgggg atggagaata cataatctac 1140
acagcaatgg ctttaagaaa caaagcgttt ggtagcgcac aagaatttgt gtgggctcaa 1200
gattccagcg aatatgccat cagagaatcc ggatctacta tcagaatttt taagaatttc 1260
aaagagaaga agaattttaa gtccgatttt ggagctgaag gtatatacgg tggatacctt 1320
ttgggagtca aatcggtttc tggtttgact ttctatgatt gggaaactct cgatttagtc 1380
agaagaatcg agatacaacc aaaagcagtt tactggtcag atagtggtaa attagtatgt 1440
ttggccacag aagatagcta ctttattctt tcttatgatt ctgatgaagt tcaaaaagcc 1500
agagataaca atcaggttgc ggatgatgga gtagaatcgg ctttcaatct tctaggtgaa 1560
ataaacgaat cagtgcgaac tggtctctgg gtaggcgact gttttatcta cacgaattct 1620
gttaatcgta tcaactactt cgttggaggt gaactggtta caattgctca tttggaccgg 1680
cctttgtatg tcttgggata tgtgcctaaa gacgatagat tatacctcgt agataaagag 1740
ttgcgcgtag taagctacca attacttctt tctgttcttg aatatcaaac tgccgtcatg 1800
agaagagact ttccaacagc agacagagta cttccgtcca ttcctaagga gcacagaacg 1860
agagtggcac atttcttaga aaagcaaggc ttcaaacagc aagctttggc cgtaagtaca 1920
gatccagagc acagattcga gctggcagta gcattagagg atcttaatat agccaaaact 1980
ctagctcaag aagcgaacag tccgcaaaag tggaatcaac tagcagaatt ggcagctgct 2040
actaataatg taagcgtagc caaggaatgt atgcaaaaag cgcaagatta tggaggcttg 2100
ttgcttcttg ctacgagctc cggtgatgaa aatttagtcc gtactctagg agaaacgaca 2160
caagctgaaa gcaaacataa cttagcattt ttgtcacact tgttagtagg tgatttaaac 2220
aaatgtctag acattcttat taataccggt agattgccag aagctgcatt tttcgccaga 2280
tcttaccttc ctgataagat tacagaagtc gtggaactgt ggaagactca gttatcttca 2340
gtcaatcaaa aagctggaca gagccttgcc gatcctaaaa actacgaaaa tctgttccct 2400
ggtttacaag aggcggtggt agctcagaaa tttttggaac agcagaataa aggtttagcg 2460
cccgcaagag ttgccaccac cattcctcct aatcacgaca ggaatgttgt agccgaagtt 2520
caagcacaat cgaaacacga tgtaccatca tttagttctt cgtttatttc atcagaaata 2580
gaagcacaaa caaggagttc tgctaaacct gaagaatctt caaacattat acagctggac 2640
caagatgacg acgatatcga tttagatttg gacggtgtaa atatcgatga gaacattgac 2700
acgacggata tcaacatcga tgatgatttg ctgagtgatt ga 2742
<210> 94
<211> 1146
<212> DNA
<213> 玉米根萤叶甲属
<400> 94
atgcagatct ttgtaaaaac actcactggt aaaaccatca ccctcgaggt tgaaccatca 60
gataccatcg agaatgtcaa agctaaaatt caagacaaag aaggtattcc accagatcaa 120
cagagattaa tctttgctgg aaagcagtta gaagatggcc gtactctctc agactacaac 180
attcagaaag aatctacact acacttagtg cttcgtctta gaggaggtat gcacatcttt 240
gtaaaaactc tcactggtaa gaccatcacc cttgaggttg aaccatcaga taccatcgag 300
aatgtcaaag ctaaaattca agacaaagaa ggtattccac cagatcaaca gagattaatc 360
tttgctggaa agcagttgga agatggccgt actctctcag actacaacat tcaaaaagag 420
tctaccctcc atttggtact tcgtcttaga ggaggtatgc agatttttgt taaaacttta 480
actggaaaga ccatcaccct tgaagtagaa ccttctgata ccatcgaaaa tgtcaaagcc 540
aaaattcaag acaaagaagg tattccacca gatcaacaaa gattaatctt tgccggaaag 600
caattggaag atggtcgtac actctcagac tacaacattc aaaaggaatc taccctccat 660
ttggtacttc gtcttagagg aggtatgcaa atctttgtaa aaacactcac tggtaagacc 720
atcaccctcg aggttgaacc atcagatacc atcgagaatg tcaaagctaa aattcaagac 780
aaagaaggta ttccaccaga tcaacagaga ttaatcttcg ctggaaagca gttggaagat 840
ggccgtactc tctcagacta caatattcag aaagagtcta ccctccattt ggtacttcgt 900
cttagaggag gtatgcaaat ctttgtaaaa actctcactg gtaagaccat caccctcgag 960
gttgaaccat cagataccat cgagaatgtc aaagctaaaa ttcaagacaa agaaggtatt 1020
ccaccagatc aacaaagatt aatctttgcc ggaaagcagt tggaagatgg ccgtactctc 1080
tcagactaca acattcaaaa agagtctacc cttcacttgg tacttcgttt aagaggagga 1140
aattaa 1146
<210> 95
<211> 1131
<212> DNA
<213> 玉米根萤叶甲属
<400> 95
atgtgtgacg acgatgtagc ggctcttgtc gtcgacaatg gctccggaat gtgcaaagcc 60
ggtttcgccg gtgatgacgc ccctcgtgct gtctttccat ccatcgtagg tcgtcccaga 120
caccaaggtg tcatggtggg tatgggtcaa aaagactcct acgtaggaga cgaagcccaa 180
agcaaaagag gtatcctcac cttaaaatac cccattgaac acggaattat cactaactgg 240
gacgatatgg aaaagatctg gcatcacacc ttctacaatg aacttagagt agcccccgaa 300
gaacatccca ttcttttgac tgaagctcca cttaacccaa aagccaacag agaaaagatg 360
actcaaatca tgtttgaaac tttcaatacc cctgccatgt atgttgccat tcaagctgta 420
ttgtctctgt acgcttccgg tcgtaccact ggtattgtac ttgattctgg agatggtgta 480
tcccacacag tacccatcta tgaaggttac gctctcccac acgccatctt gcgtttggac 540
ttggccggta gagacttgac tgactacctt atgaagatct taaccgaaag aggttactct 600
ttcaccacca cagctgaaag agaaatagtt cgtgacatca aggaaaaatt gtgctatgta 660
gctttggact tcgaacagga aatggccaca gcagccagct ccacctcctt agaaaagagt 720
tatgaacttc ctgacggtca agtcatcacc attggtaatg aaaggttccg ttgccctgaa 780
gctctcttcc aaccttcctt cttgggtatg gaatcttgcg gtatccacga aactgtctac 840
aactccatca tgaagtgcga tgtcgacatc cgtaaagact tgtacgccaa cactgtcctt 900
tctggaggta ccacaatgta ccctggtatt gccgatcgta tgcaaaagga aatcactgcc 960
ttggctccat caaccatcaa aatcaagatc atcgctcccc cagaaagaaa gtactccgtt 1020
tggatcggtg gctccatctt ggcctccctc tccaccttcc aacagatgtg gatctccaaa 1080
caagaatacg acgaatccgg ccctggaatt gttcaccgca aatgcttcta a 1131
<210> 96
<211> 2640
<212> DNA
<213> 玉米根萤叶甲属
<400> 96
atgggtactt ttaaaagaga tactcatgat gaggacgggg gatcaagtgc ttttcaaaat 60
ctggagaaaa ctactgtttt gcaggaagct agagttttta atgaaactag tgtaaatcca 120
agaaaatgta caccgatact aacaaaactg ttgtacttat tgaaccaggg tgaaacttta 180
agtgccaaag aggccacaga tgttttcttt gccatgacca aactgttcca atcaaaagat 240
gtaatattga gaaggatggt ttatttggga attaaagaac tcagttctgt tgctgatgat 300
gtcattattg taacatccag tcttacaaaa gatatgactg gtaaagaaga catgtacaga 360
gcagctgcta taagagcatt atgcagtatt actgatgcta ctatgcttca agctatagaa 420
cgttatatga agcaagctat tgtagataga aacgcagctg tcagttcagc agcactaatt 480
agttcattac atatgagcaa attagctcca gatgtagtaa aaagatgggt aaatgaagct 540
caggaagcag taaatagtga taatgcaatg gtacagtatc acgcattagg tcttctatac 600
catattagga agactgataa gctagcagtg acaaaattga tttccaagct gaattcaatg 660
ggtttaaaga gcccttatgc tttgtgtatg ttgataagaa tcactgcaaa acttttagaa 720
gaagaggacc aagagtcact cctcaactcc ccatatacaa taatatttac aatgggctta 780
aggaacaaat ctgaaatggt ggtgtatgaa gctgcacatg ccatggttaa cctgaagttc 840
acgagtagta atgtgctagc acccgctata agtgttctac aactattttg tggatctcct 900
aaagccacac tcagatttgc tgctgttaga actttaaatc aagtggccac cacccaccct 960
gcgtcagtga cagcttgtaa tttggatcta gaaaatttga ttactgatcc taataggtca 1020
attgctacac tggccattac tactcttttg aaaacaggtg ccgaatcttc tgttgacaga 1080
ctaatgaaac aaatcgctac ttttgtatct gaaatcagtg atgaatttaa agtggttgtc 1140
attcaggcaa ttaaggtatt agctttgaaa tttccaagga aacatagcac gcttatgaat 1200
ttcctatccg ccatgttaag agatgaggga ggtttagaat ataaagcatc catagcagat 1260
accattataa ccctaatcga agataatccc gaagctaaag aatctggttt ggcgcatctt 1320
tgcgagttca ttgaagactg tgaacatgtt tctttggctg tgagaatctt gcatttgtta 1380
ggaaaggaag gacccaagac caaacaacca tcgagataca tccgttttat ctacaatcgc 1440
gtcatattgg aatgtccttc tgtaagagct gctgcagtct ccgccatggc acaattcgga 1500
gcctcttgtc ccgatttgtt agaaaatatc caaatattac tttcgaggtg tcagatggat 1560
tcagacgatg aagttaggga cagagctaca tattatagta atatacttaa caaaaatgat 1620
aaaagtttat acaacaatta cattttggat tctttgcagg tttcaattcc ttcactagaa 1680
agatcgctta gagaatacat tcaaaatcca actgacgaac catttgacat taagtccgta 1740
cctgtagcag cagtgccaac agcagaagaa cgagaagtta aaaacaaatc tgaaggactg 1800
ctagtctctc aaggtccagt ccgacctcct ccggtgtcta gagaagaaaa cttcgccgaa 1860
aaacttagta acgttccggg tatacaacag ttaggacctt tgttcaaaac ttccgacgtc 1920
gttgaactca ctgaatctga aacagagtat tttgtccgct gtatcaagca ctgtttcaaa 1980
catcacatcg tcctccaatt cgattgtctg aataccttgc cagaccagct tttagaaaac 2040
gttagagtgg agatagacgc cggtgaaacc ttcgaaattt tggcagaaat accttgtgaa 2100
aagttgcact ataacgaaac cggtaccaca tatgtagtag ttaagttgcc tgatgatgat 2160
ctccccaact ctgttggtac gtgtggagcc gtgttgaagt tcttagtgaa agattgtgat 2220
ccatcaacgg gaataccaga ttctgatgag ggttacgatg atgaatatac actggaagac 2280
atcgaaataa cattagggga ccaaattcaa aaagtaagca aagtaaattg ggctgcagcc 2340
tgggaagaag ctgcagctac ttatgtagaa aaagaggata catactcctt gaccatcaat 2400
acgctaagtg gcgctgttaa gaatattatt cagttcttgg gattacagcc tgcggaaagg 2460
actgacagag taccggaggg taaatctacg cacacattac ttcttgctgg tgtattcagg 2520
ggaggtattg acatactagt aagagcgaaa ctagctttgg gcgaatgtgt tacgatgcaa 2580
ctaacagtca ggtcgccaga tcctgacgtt gctgagctta taacttcaac tgtaggttaa 2640
<210> 97
<211> 651
<212> DNA
<213> 玉米根萤叶甲属
<400> 97
atggcggcaa acagaactgg acctgctcag agaccaaatg gcgctaccca aggaaagata 60
tgtcagttca aactggtcct actaggcgaa agtgccgtcg gtaagtcgag tttggtactg 120
aggttcgtca aaggacagtt ccacgaatac caggagagta ccataggagc agctttcctt 180
acacaaacca tatgcctcga cgatacaact gttaaatttg aaatttggga cacagcgggt 240
caagaaaggt accacagttt agctcctatg tactataggg gcgcacaggc agctatagtc 300
gtctacgaca taaccaatca agacacattc ggcagggcga aaacgtgggt gaaggaactt 360
caaaggcagg ccagtccgac gatcgtgata gctttggccg gcaacaagca agatttggcc 420
aacaaacgta tggtagaata cgaagaggcg cagacgtatg ctgacgaaaa cggcttactt 480
tttatggaaa cttccgcaaa gacggcaatg aacgtcaacg atatattttt agcaatagct 540
aagaaactgc ccaagaatga acaaaccaca ggtcaaggcg gcagtgccca aggcaggcgg 600
ctagcggagg gcgattcggg cgccaaggca cccggaaatt gttgcaagtg a 651
<210> 98
<211> 279
<212> DNA
<213> 玉米根萤叶甲属
<400> 98
atgaagtttt taagatcgac agtgtgctac attgccatct tggcaattct ctttaccctc 60
tgtgccgatg aggttgaagg aaggagaaaa attttgatgg ggcgaaaaag cattaccagg 120
acatatcttc gtggaaatgc tgttcctgcg tatgtgataa taatccttgt aggaattggt 180
caaatcatcc tgggagggat attgtacgtt gcattgagga agaagatcat tgctgcacct 240
gtaacggcat catatgcagt ggctagacaa gaaccataa 279
<210> 99
<211> 474
<212> DNA
<213> 玉米根萤叶甲属
<400> 99
atgcagatct tcgttaaaac cttaacgggt aagaccatca ctcttgaggt cgagccctca 60
gatactatcg aaaatgtgaa agctaaaatc caggataaag aaggaattcc cccagaccag 120
caacgtctca tcttcgctgg aaaacaactc gaagatggtc gtaccttgtc tgactataat 180
attcaaaaag aatcaaccct tcacttggtg ttgagattga gaggaggtgc taagaaacgt 240
aagaagaaga attactccac ccccaagaaa atcaagcaca agaagaagaa ggttaagtta 300
gctgtattga aattttataa ggttgacgaa aatggtaaaa tccaccgatt gagacgtgaa 360
tgccccgctg aacaatgtgg agctggtgtc ttcatggcag ccatggaaga caggcattac 420
tgtggcaagt gcggttacac tcttgtcttc tccaaaccag gagatgagaa atag 474
<210> 100
<211> 747
<212> DNA
<213> 玉米根萤叶甲属
<400> 100
atgatgtcca aagcagacac acaggaagat gcctccttcg ccaaattgga aaatcagatt 60
gctatcatca aatacgtaat actctttacc aacgttttgc aatgggctct cggtgcagca 120
atcttcgctc tttgcctttg gctacgattc gaggagggca ttcaagaatg gctccagaaa 180
ttggattcag aacaatttta catcggagta tatgtactta tagtcgcttc actgatcgtc 240
atgattgtgt cctttatagg atgtattagt gccctgcagg agagtaccat ggccctttta 300
gtgtacatcg gcacccaagt gctcagtttt atattcggtt tatccggttc ggcggttctt 360
ctggataaca gcgccagaga ttcccacttc caaccgagga tccgagagag tatgcgacgt 420
cttatcatga atgctcatca cgaccaatcc agacaaacac tagccatgat tcaggaaaat 480
gttggttgct gcggagctga tggcgcaaca gactacctct ctcttcagca gccccttcca 540
agtcagtgca gagacaccgt tactggaaac ccattcttcc acggatgtgt agatgaactc 600
acctggttct tcgaagaaaa atgtggttgg atagcaggtt tagctatggc gatatgcatg 660
attaacgtcc ttagtattgt tttatctacg gtactcatcc aggcattgaa aaaagaagaa 720
gaagcatccg attcatacag gagatag 747
<210> 101
<211> 375
<212> DNA
<213> 玉米根萤叶甲属
<400> 101
atgtctggac gtggcaaggg aggcaaagta aagggaaaag caaagtcccg atcaaatcgt 60
gctggtttac aatttcctgt aggtcgtatt catcgtttat tgagaaaagg aaattatgcc 120
gaaagagttg gtgctggagc tcctgtatac ttggcagctg ttatggaata tttagctgct 180
gaagttttgg aattggcagg aaatgcagct agagataaca aaaagacccg tataattcct 240
agacatttac aattggccat aagaaatgac gaggaattga acaaattact gtcaggagtt 300
accatcgccc aaggtggagt attgcctaat atacaagcag tactgttacc taaaaaaact 360
gaaaagaaag cttaa 375
<210> 102
<211> 864
<212> DNA
<213> 玉米根萤叶甲属
<400> 102
atgaagttgg atggtgtaga tctgccccca ccaattagct tcgacattgc ggaagagcaa 60
ccgttaccac cttgccaaca gacgttctta tgtaatggtg atggaggatc catagtgcga 120
cagtttctcg agctgtattt cgtaatatat gattcagata ataggcagtc ccttcttcag 180
gcatatcacg aaaaagccac attttcaatg acaatggcct acccgtacgg ctattccaaa 240
gacagtaaag gagtatcgtg gttgaattgg tatgccaccg ataatagaaa tttattacga 300
gttcaagatc cagacagaag aaacaagttg ttaagacagg gacaagttgc tgtagtttcg 360
ttcttgcaag atatgccgca cacgaagcac gatattcaca gttttacagt agatttgaca 420
gtttttacac cccagatgtt atgtttgaca gtggctggta tgtttaaaga attgaaaagt 480
ggccacaaag tacctccttt aagatatttc ttcagaaccc ttgtaattgt acctgctgga 540
tcaggttttt gcatagcaaa tgaagaactt cacatatcca atgcaactcc ggaccaagca 600
aaagatgctt tcaagaccac cgttaatgta gctccggcac cagcccctgt gattacctct 660
cctggaccca gtataccaca acccgctgtg ccagatgatg ctacaaaaca agaaatggta 720
aaacagatgt ccgcagtatc cggaatgaat ctcgagtggt cgctacagtg tctcgaagaa 780
acacaatggg actaccagaa agccataatg gtattccaaa atttaaacgc acaaggtgtt 840
gtaccacaag cagcatttat taaa 864
<210> 103
<211> 2868
<212> DNA
<213> 玉米根萤叶甲属
<400> 103
atgactgcgg tagaacaacc ttgttacaca ctaataaact tgccaacaga ttcggagccc 60
tacaatgaaa tgcaactaaa aatggattta gaaaagggtg aggttaaagt aaaaataaga 120
gcattagaaa aaataattca catgattctg gcaggagaaa ggttgccgaa tggatttcta 180
atgaccatca taagaaacgt tttaccttta caagatcatt tggcaaaaaa actattattg 240
attttctggg aaatagttcc aaaaacaaat ccagaaggta aactactaca agagatgatt 300
ttggtatgtg atgcctatag aaaagatctg caacacccaa atgaattttt gagaggttct 360
acacttcgct tcttgtgcaa actgaaggaa ccagaattgt tggaaccatt aatgcccagt 420
attagagctt gtttggatca taggcacagc tatgtgagga ggaatgctgt actggcaatt 480
tttaccattt acaaaaattt tgaagccctc attccagatg ctcctgaact gatctccaat 540
tatttggatg gtgagcaaga catgtcttgt aaaagaaatg cgtttttaat gcttcttcat 600
gctgaccaag aaagggcgtt gtcgtatttg gcatcatgtt tagatcaagt aaattcattt 660
ggagatattc tacaactggt catcgttgag ttgatatata aggtgtgtca ttccaatcct 720
gcggaaagat ctagatttat tagatgtata tataacttgt tgaactcaag cagtcctgct 780
gtcaggtacg aagctgcagg aactttagtc accctctcca gtgccccgac tgccgttaaa 840
gctgctgcta gctgttacat tgagttaatt atcaaagaaa gtgacaacaa tgtaaaactc 900
atcgttttgg acaggctgat agcacttaag gagcttccta atcacgaaag aattctgcag 960
gatttagtta tggacatact gagagtactc tctgctcctg acttagaagt ccgcaagaag 1020
actttaagtc tagcccttga attagtctct tcacggaaca tagaagaaat ggtattagta 1080
ttaacaaagg aagtgagtaa aacggtagac agtgaacatg aggatacagg aaagtacagg 1140
caattgttag taaggactct acattcgtgt tccattaagt tcccagatat cgcacgtagt 1200
gttataccag tcttgattga atttttatcc gataataatg aactggctgc cacagatgta 1260
ttgctgttct taagggaagc catacagaag tttaaagaat tgcaaccgtt aattattgag 1320
aaactcatcg aaactttcaa agacattaaa ttggtcaaag tccatagagc agcaatttgg 1380
attttgggag aatacgcgag tactgcttcc gatatagaag ttatcgttgg agaaattaac 1440
agattgttgg gtgaaggatc cctcgttgaa gctgagcaga agttaatagc aggagatacg 1500
gaagagaatg ctcctgcacc tgctgcaggc gccaccactt tagttacttc cgatggaaca 1560
tatgctaccc aatcagcttt caacactgtc agccaaacca ctaaagaagc acgacctcct 1620
ctaagacaat acctcatgga tggtgatttt ttcatcggag cctctttggc atctacatta 1680
accaaactgt ctttgcggta tgaggacctc acctctcctg ctgctagcaa tggattcaat 1740
gccaaaatta tgcttattat ggctggaatt cttcacttgg gaaaatcagg acttcccaca 1800
aaatcaataa ccaacgacga taaagaccac attctgttct gtttacgagt cctatctgat 1860
cgttctccaa tcattgttga aattttcaaa aaattgtgcc gctcggcact aaatgagatg 1920
cttctagcta aggaatcggt agaagcgatc tcgcaaaaga gcaaagaaaa aaacaagcgt 1980
acgattcaaa ctgacgacgc tataagcttc ctgcaattag agacagataa aagtggagag 2040
ctaggagaaa acgtattcga gatgtcgctg tcacaagctt tagtaggagg tcgaacggga 2100
ggtggcgaat cagtattaag ttccaataaa ttagataaaa tcacacaact gactggtttt 2160
tccgatccag tttattccga agcatacgtt cacgtgaatc agtacgatat cgtgcttgat 2220
gtcttaatcg taaaccaaac taacgatact ttacaaaact gcacgctaga gctggctact 2280
ttaggcgatt tgaagttggt agagaagcca caacctgtcg tattggcgcc caaagacttt 2340
tgcaacatta aagctaacgt gaaagtggcc tcaactgaaa acggaattat atttggcaac 2400
attgtgtatg atgtcatagg agcggggtca gataggaatg ttgtagtttt gaatgatata 2460
cacatagata taatggacta tatagtgcct gctagttgta cagatagcga gtttatgaga 2520
atgtgggcgg aatttgaatg ggaaaataag gtaaccgtta acacacccct cacggaactt 2580
tcagaatacc tcgaacatct actcaaaagc acaaatttga aatgtttaac atcagaaaaa 2640
gctctgagcg ggcagtgtgg ttttatggca gccaatttat atgcaaaatc catttttgga 2700
gaagacgctt tggccaactt aagtatagag aaacctttta ataaacccga tgcgccagta 2760
agcggtcata ttagaataag ggccaaaagt cagggcatgg ccttaagttt aggagacaaa 2820
gtcaatatga cacagaagag cacacaacat aaagtagtag ctgcataa 2868
<210> 104
<211> 549
<212> DNA
<213> 玉米根萤叶甲属
<400> 104
atgggtaatg tgtttgcaaa tttattcaaa ggcctctttg gcaaaaagga aatgaggata 60
ttgatggtag gactcgatgc agctggtaaa accacaattt tatataaact taaattagga 120
gaaattgtaa caactattcc aacaattgga tttaatgtgg agactgtaga atataagaac 180
attagtttta cagtatggga tgtaggtggt caagataaaa ttaggccatt gtggagacac 240
tatttccaaa acacacaagg cctaattttc gtagtagaca gtaacgacag ggaacgtatc 300
actgaggcta aagatgaatt aatgcgtatg ttggccgaag atgaacttag agatgccgta 360
cttctcattt tcgccaacaa acaagatttg cccaatgcaa tgaacgctgc agaaatcacc 420
gacaaactcg gtctccattc actacgcaac cgcaactggt acattcaagc tacctgtgca 480
actagcggag atggtctcta tgaaggtctg gactggttgt ccaatcaatt aaagaacgcc 540
aatcgctag 549
<210> 105
<211> 4425
<212> DNA
<213> 玉米根萤叶甲属
<400> 105
atgggacggt tgcactgttt attttgtatt ttcctatgtt ttaccgtcat caacacgcag 60
acaacgaata tacatggatt ctcggaaaat tccgtggata catttctatc acctcatggg 120
aaaagtgcaa aattcgtgca ccaaaatcac aaacccaaaa ttgaaaattg tcagaactac 180
aaaccctcgg tgaaagaaga acagccaggc ggaacgtacg taacaacggt taccgctatc 240
gatgacgatc ctagggaggg aggaggaaca attagttaca aactaattca tagagaagga 300
gaacatgttt tatttgacat agacaacgtt actggtgttt tgacaactat ccagccattt 360
gatcgggatg aaccagtaag gcagaaggaa ctttatgtaa ccgtacaagc ttcagacaac 420
ggcaggccac cattagcaga tgtctgtaca tttacagtta ccattaccga cattaatgat 480
aatgcgccac agcttgataa actgaaatac gatgcacaag tttctgaaga tttaaaagta 540
ggaagtgaag tgatgagagt ttttgcttac gacattgatg atggggaaaa ttcaagatta 600
tcgtataact tttcaaacga aaatgctcaa ttcacccagt atttcaggat agatcgagat 660
actggcgttg tgtatttaaa ggaagcttta acagacaaaa agaatactag atttaacagt 720
gctgtttatg tagccgataa tggcgttaac gatcaagaag gccaaaaaga ttcaaccgct 780
aagatatcta taacagtagt agggtctgat aaacagcctc ccagatttac tcaaaaaatg 840
cctgatggaa tcttggagat ccccgaagat tttaaagact tttctaaaca tattgtcaca 900
gtcgaagcaa cgtccaacat tgcggatcca caacttgctt ttgaattggt gaagggaaag 960
acatatcaaa ccaataaaga ccaaacgttt cttttggagg cagaaggaaa taaagcgcac 1020
ataaagctag tgcgtccact ggattatgaa acagtaacgg aatatactct aactattcga 1080
gtaaaaaaca aagatttaat ggattcttcc ataaatatac caattaaagt attagatgtt 1140
aatgatgaaa ttcctaattt ccttgaattt cttaaaggta gtgtcgtgga aaatgacaag 1200
ccaggtgcac aagcgattca agtaagagca atcgataaag acggaactgc tgctaacaac 1260
attgtgagct atgaactcgt tgacaataca gatttgtttg caataaaccg atctacggga 1320
gtaattacgt cgagagtgga gtttgatcgt gaaactgtac ctctatatca cgtaaacgtt 1380
aaagcttatg ataactctcc gtctgctttg tataacacga cattgcctaa cattgtaatt 1440
cagacattcc aaatcagtat agaagatcaa aatgacaaca aacctgtatt tactcatcca 1500
atttatcagt tcagtaatat tactgagctt gctgataaat cgagtattgt tggtgaagtc 1560
aaagctttag ataatgacac ggcttcagtt ataagttata gtattacaaa tggaaatatt 1620
gacgatgcgt ttatgattga aaattctacc ggcagaataa gagttaatgg aaaactggat 1680
tacgagaaaa tcgaacaata caacttaacc gttcgcgcat ttgatggggc atttgaagat 1740
tttgcaattg ttttaatttc catacttaat gaaaatgacg aacctccagt ttttgacgac 1800
tatatcagag aaattcaaat taaagaggaa gaacctatga tatccggatg cgttgttaga 1860
gtgactgctc atgatccaga tattaaagac aggcatgctg atcaacacat agtatatgag 1920
gtcgcgaaag aacagaaaga ttttttgacc gtatctgccg atggatgcgt acaagtaaca 1980
aaacctctcg accgagatcc gcctttcggt agcccaacac gacaagtctt catctatgct 2040
cgtgataatg atggaggcac aaattcattg ttggccactg cagaaattga aattatttta 2100
atagatataa acgataatgc tcccttttta aatgttacag aaattgttta ttatgaaaac 2160
caggatccag gttttatagg taacctaagt gccgatgatt acgatggtcc tgataatgga 2220
cctccgtttg cttttcgatt atcagacact gcttcagata gtattagatc gaaattttcc 2280
attatcggaa accagctttt cgctttagaa atgtttgata gagaagagca aaaatattat 2340
gacattgcca ttgacattac agatagtgga gtacctccac taacaggaac tagtattctt 2400
agagttataa tcggagatgt aaatgataat ccagctacag acggaaacag cacgatcttt 2460
gtgtataagt acgtcaatgg gccagaaaat ttcatggaaa tcggacgtgt atatgttaca 2520
gacctagacg attgggattt aaatgacaaa gtctttgttc aagaagataa ctttgatgaa 2580
tttgtgttaa accagcataa caacggtatg attctgatga aaccaacaac ggctgaggga 2640
acttatgagg ttcattacag ggtcactgaa acccatgaac ccacaataca cgaacataca 2700
gttaatgcaa tagtcacgat tacagttaaa gtacttccag aggaagcggt tgtaaaatca 2760
ggatcaattc gattgagagg aacaactaag gaagaattca tagaaaattc attgaatgga 2820
aagagcaaaa gagacatatt acaccaagaa ctctccaaaa tattaaatac atctttagcg 2880
aatgttgatg tatttactgt tttaaattca ccccaccaga atagttcgtt tgtggatgtt 2940
cgattttctg ctcatggatc tccatattat gctccagaga aactcgaaaa caaagttaca 3000
gatcatcaaa tggagcttga acaaaaatta gatgtggaat tctacatgat caacgtaaac 3060
gagtgcctta acgaaacaac gtgtggagct gaaaactcat gtacgaacaa attaaacata 3120
acacgagaac cagctgtagt gtttactaac agaacatcct ttgtcggtgt aaatgcattt 3180
attgatcctg tgtgtgccgc tttaccaaga gatgttatgg aatgtttcaa cggaggcgtc 3240
cttatcgaaa acacagcgtg taattgtcct gcaggatttg aaggaccaca ttgtgaaatc 3300
ctagctatag gatttacagg aactggttgg gctatgtatc catcctttga cgctacaaac 3360
aggactgaga ttatactgca tattttatca caaactgata atggtttgat attttacaat 3420
ggacctttaa atataagaca aacttctttg tctaaagatt atatatcatt agaacttaaa 3480
gacggatatc cattacttca aatttgcacc ggctcaagca ctcaagaaat ttatctgaaa 3540
gagcgcattc acaaattgag cgatggatcg ttacacaaaa taaaaatagg atctggattt 3600
gacgatatat ccctggaagt agacgactgt ggaacaacgt gttcaatttg gactaataaa 3660
ctacataaag gtgttatccg agcaaatggc ccccttcaac tgggaggtat gaaaaacaga 3720
ttcaccgatc aagaattcaa acgaatttgg gaccatttgc caccgactgc cacccgtttc 3780
tctggttgta ttagaaattt gacgtataat gaattttact acaacctcgg tgcaccttct 3840
gatgcattcc aagcgtatcc cgactgtaac tatgcagtga tgcaagctgt gactttcggt 3900
atcgactcca atttcttggt tgctattctg gtttgtgtag caattttgat aattcttctt 3960
ctggcagtag ttgtacatag acgtaaacac gacaacttta acgaaaaaga aatcgatgat 4020
actcgcgaaa acattatcaa ctacgaagat gaaggtggcg gcgaatgtga caccaactac 4080
gacctgtctg ttttccatca gaacaacatt gtggacgaaa aaccattgat gagagacaac 4140
cccgatgtac ctgcagatat aagtggcttt ttagataaca agaaagacaa ctgtgataaa 4200
gaccccgata atttgcctta tgacgacgtt cgccattatg cctacgaggg agacggaaat 4260
agcaccggat ccttatcttc tctcgcttca tgtacggacg aaggagattt aaagttcaac 4320
tacttatcaa gttttggacc cagattcaga aagttagccg acatgtatgg agaagatcca 4380
agcgatgaag actcacacga tggaaacgaa gaatcctggt gctag 4425
<210> 106
<211> 291
<212> DNA
<213> 玉米根萤叶甲属
<400> 106
atgcctttct gtggtcccaa attgtccctc tgcggcctga ttatcagtgc atggggtatc 60
atccagttgg gtttcatggg tgtattctat tacattgggg ctgtggcttt agcagaagat 120
attccagagg ttgagtttaa gggcgattta gacaaatttt atagcgacgt caacacgggt 180
ttcacacaga atgcttacaa ctgctggatt gctgctctcc tatacctgat aacattagca 240
gtatcagctc accaattctg ggccaacaac agatcatcat tgaacgtcta a 291
<210> 107
<211> 540
<212> DNA
<213> 玉米根萤叶甲属
<400> 107
atgggtctta ccatatcagc agtgtttaat aggttgttta gtaaaaagcc tatgagaatt 60
ttaatggtag gattagatgc cgcaggtaaa accacaatct tatacaaatt gaagcttggt 120
gaaatcgtaa ctacaatacc aaccatcggc ttcaatgtag aaaccgttga gtacaagaat 180
atatctttca cggtatggga tgtaggtggc cagacgagaa tcagaaaact ctggagacac 240
tatttcgcca acactgatgg actcattttt gtggttgatt ccaacgaccg agaccgtatc 300
gcggaagccg aagaagaatt gcacaatatg ttaggagagg acgatttaag agactgcatt 360
ttgttaatat tcgccaacaa acaagattta ccgaactcga tgtccactgc tgaattgacc 420
gataagctta agttgcacac tttgaagaat aggaggtggt acatacaagc cacatgtgct 480
actcaaggga atggtttgta cgaaggacta gattggttgt cgaatgaatt ggccaagtga 540
<210> 108
<211> 618
<212> DNA
<213> 玉米根萤叶甲属
<400> 108
atgcggtaca ctttgagtta catcggtgct accctagcgt ccactgtaac actgattttt 60
gccctctact actgcctcac gggaaaagga gagcaagtta gtttagcatg gttattgttg 120
aatgtgtctc cccacatgtg ggcaggtcta ggaattggcc ttgctgtatc attatcagtt 180
gtaggagctg ctgcaggaat tcacactaca ggagtcagta tcgtaggagc tggtgttaaa 240
gcccccagaa tcaaaaccaa aaatttaatt tctattattt tctgtgaagc tgtggctatc 300
tatgggttaa ttatggctat agtactctgt ggaagttgga agaatttcga tgtagaccta 360
ttcaacctca aaactcataa ctttgctcaa aaccattatg gatcacatgt tatttttgga 420
tccggtttaa ctgttggatt tgtaaatcta ttatgtggat tttgtgttgg agtagttggt 480
tctggtgcag ccatttctga tgcagccaat tcatcattat tcgtcaaaat tttgattatt 540
gagatttttg gaagtgccat tggtctcttc ggtctgattg ttggagtata cttgacgtca 600
agaggctcta tggtttaa 618
<210> 109
<211> 5694
<212> DNA
<213> 玉米根萤叶甲属
<400> 109
atggctacca acgatagtaa agctccgttg aggacagtta aaagagtgca atttggaata 60
cttagtccag atgaaattag acgaatgtca gtcacagaag ggggcatccg cttcccagaa 120
accatggaag caggccgccc caaactatgc ggtcttatgg accccagaca aggtgtcata 180
gacagaagct caagatgcca gacatgtgcc ggaaatatga cagaatgtcc tggacatttc 240
ggacatatcg agctggcaaa accagttttc cacgtaggat tcgtaacaaa aacaataaag 300
atcttgagat gcgtttgctt cttttgcagt aaattattag tcagtccaaa taatccgaaa 360
attaaagaag ttgtaatgaa atcaaaggga cagccacgta aaagattagc tttcgtttat 420
gatctgtgta aaggtaaaaa tatttgtgaa ggtggagatg aaatggatgt gggtaaagaa 480
agcgaagatc ccaataaaaa agcaggccat ggtggttgtg gtcgatatca accaaatatc 540
agacgtgccg gtttagattt aacagcagaa tggaaacacg tcaatgaaga cacacaagaa 600
aagaaaatcg cactatctgc cgaacgtgtc tgggaaatcc taaaacatat cacagatgaa 660
gaatgtttca ttcttggtat ggatcccaaa tttgctagac cagattggat gatagtaacg 720
gtacttcctg ttcctcccct agcagtacga cctgctgtag ttatgcacgg atctgcaagg 780
aatcaggatg atatcactca caaattggcc gacattatca aggcgaataa cgaattacag 840
aagaacgagt ctgcaggtgc agccgctcat ataatcacag aaaatattaa gatgttgcaa 900
tttcacgtcg ccactttagt tgacaacgat atgccgggaa tgccgagagc aatgcaaaaa 960
tctggaaaac ccctaaaagc tatcaaagct cggctgaaag gtaaagaagg aaggattcga 1020
ggtaacctta tgggaaagcg tgtggacttt tctgcacgta ctgtcatcac accagatccc 1080
aatttacgta tcgaccaagt aggagtgcct agaagtattg ctcaaaacat gacgtttcca 1140
gaaatcgtca cacctttcaa ttttgacaaa atgttggaat tggtacagag aggtaattct 1200
cagtatccag gagctaagta tatcatcaga gacaatggag agaggattga tttacgtttc 1260
cacccaaaac cgtcagattt acatttgcag tgtggttata aggtagaaag acacatcaga 1320
gacggcgatc tagtaatctt caaccgtcaa ccaaccctcc acaagatgag tatgatgggc 1380
cacagagtca aagtcttacc ctggtcgacg ttccgtatga atctctcgtg cacctctccc 1440
tacaacgccg attttgacgg cgacgaaatg aacctccatg tgccccaaag tatggaaact 1500
cgagctgaag tcgaaaacct ccacatcact cccaggcaaa tcattactcc gcaagctaac 1560
caacccgtca tgggtattgt acaagatacg ttgacagctg ttaggaagat gacaaaaagg 1620
gatgtattca tcgagaagga acaaatgatg aatatattga tgttcttgcc aatttgggat 1680
ggtaaaatgc cccgtccagc catcctcaaa cccaaaccgt tgtggacagg aaaacagata 1740
ttttccctga tcattcctgg caatgtaaat atgatacgta cccattctac gcatccagac 1800
gacgaggacg acggtcccta taaatggata tcgccaggag atacgaaagt tatggtagaa 1860
catggagaat tggtcatggg tatattgtgt aagaaaagtc ttggaacatc agcaggttcc 1920
ctgctgcata tttgtatgtt ggaattagga cacgaagtgt gtggtagatt ttatggtaac 1980
attcaaactg taatcaacaa ctggttgttg ttagaaggtc acagcatcgg tattggagac 2040
accattgccg atcctcagac ttacacagaa attcagagag ccatcaggaa agccaaagaa 2100
gatgtaatag aagtcatcca gaaagctcac aacatggaac tggaaccgac tcccggtaat 2160
acgttgcgtc agactttcga aaatcaagta aacagaattc taaacgacgc tcgtgacaaa 2220
actggtggtt ccgctaagaa atctttgact gaatacaata acctaaaggc tatggtcgta 2280
tcgggatcca agggatccaa cattaatatt tcccaggtta ttgcttgcgt gggtcaacag 2340
aacgtagaag gtaaacgtat tccatttggc ttcagaaaac gcacgttgcc gcacttcatc 2400
aaggacgatt acggtcctga atccagaggt ttcgtagaaa attcgtatct tgccggtctc 2460
actccttcgg agttctattt ccacgctatg ggaggtcgtg aaggtcttat cgatactgct 2520
gtaaaaactg ccgaaactgg ttacatccag cgtcgtctga tcaaggctat ggagagtgta 2580
atggtacact acgacggtac cgtaagaaat tctgtaggac aacttatcca gttgagatac 2640
ggtgaggacg gactctgtgg agagatggta gagtttcaat atttagcaac ggtcaaatta 2700
agtaacaagg cgtttgagag aaaattcaga tttgatccga gtaatgaaag gtatttgaga 2760
agagttttca atgaagaagt tatcaagcaa ctgatgggtt caggggaagt catttccgaa 2820
cttgagagag aatgggaaca actccagaaa gacagagaag ccttaagaca aatcttccct 2880
agcggagaat ccaaagtagt actcccctgt aatttacaac gtatgatctg gaatgtacaa 2940
aaaattttcc acataaacaa acgagccccg acagacctgt ccccgttaag agttatccaa 3000
ggcgttcgag aattactcag gaaatgcgtc atcgtagctg gcgaggatcg tctgtccaaa 3060
caagccaacg aaaacgcaac gttactcttc cagtgtctag tcagatcgac cctctgcacc 3120
aaatgcgttt ctgaagaatt caggctcagc accgaagcct tcgagtggtt gataggagaa 3180
atcgagacga ggttccaaca agcccaagcc aatcctggag aaatggtggg cgctctggcc 3240
gcgcagtcac tgggagaacc cgctactcag atgacactga acactttcca ttttgctggt 3300
gtatcctcca agaacgtaac cctgggtgta cctagattaa aggaaattat taatatttcc 3360
aagaaaccca aggctccatc tctaaccgtg tttttaactg gtgcggctgc tagagatgcg 3420
gaaaaagcga agaatgtgtt atgcagactt gaacacacca ctcttcgtaa agtaaccgcc 3480
aacaccgcca tctattacga tcctgaccca caaaataccg tcattcctga ggatcaggag 3540
ttcgttaacg tctactatga aatgcccgat ttcgatccta cccgtatatc gccgtggttg 3600
cttcgtatcg aactggacag aaagagaatg acagataaga aactaactat ggaacaaatt 3660
gctgaaaaga tcaacgctgg gttcggggac gatttgaatt gtattttcaa cgacgacaat 3720
gctgaaaagt tggtgctgcg tatcagaatc atgaacagcg acgatggaaa attcggagaa 3780
ggtgctgatg aggacgtaga caaaatggat gacgacatgt ttttgagatg catcgaagcg 3840
aacatgctga gcgatatgac cttgcaaggt atagaagcga tttccaaggt atacatgcac 3900
ttgccacaga ctgactcgaa aaaaaggatc gtcatcactg aaacaggcga atttaaggcc 3960
atcgcagaat ggctattgga aactgacggt accagcatga tgaaagtact gtcagaaaga 4020
gacgtcgatc cggtcaggac gttttctaac gacatttgtg aaatattttc ggtacttggt 4080
atcgaggctg tgcgtaagtc tgtagagaag gaaatgaacg ctgtcctttc gttctacggt 4140
ctgtatgtaa actatcgcca tcttgccttg ctttgtgacg taatgacagc caaaggtcac 4200
ttaatggcca tcacccgtca cggtatcaac agacaagaca ctggagctct gatgaggtgt 4260
tccttcgagg aaactgtaga tgtattgatg gacgctgcca gtcatgcgga ggtcgaccca 4320
atgagaggag tatctgaaaa cattatcctc ggtcaactac caagaatggg cacaggctgc 4380
ttcgatcttt tgctggacgc cgaaaaatgt aaaatgggaa ttgccatacc tcaagcgcac 4440
agcagcgatc taatggcttc aggaatgttc tttggattag ccgctacacc cagcagtatg 4500
agtccaggtg gtgctatgac cccatggaat caagcagcta caccatacgt tggcagtatc 4560
tggtctccac agaatttaat gggcagtgga atgacaccag gtggtgccgc tttctcccca 4620
tcagctgcgt cagatgcatc aggaatgtca ccagcttatg gcggttggtc accaacacca 4680
caatctcctg caatgtcgcc atatatggct tctccacatg gacaatcgcc ttcctacagt 4740
ccatcaagtc cagcgttcca acctacttca ccatccatga cgccgacctc tcctggatat 4800
tctcccagtt ctcctggtta ttcacctacc agtctcaatt acagtccaac gagtcccagt 4860
tattcaccca cttctcagag ttactcccca acctcaccta gttactcacc gacttctcca 4920
aattattcac ctacttcccc aagctacagt ccaacatccc ctaactattc accaacatct 4980
cccaactatt cacccacttc acctagttat ccttcaactt cgccaggtta cagccccact 5040
tcacgcagct actcacccac atctcctagt tactcaggaa cttcgccctc ttattcacca 5100
acttcgccaa gttactcccc tacttctcct agttattcgc cgtcgtctcc taattactct 5160
cccacttctc caaattacag tcccacttct cctaattact caccgtcctc tcctaggtac 5220
acgcccggtt ctcctagttt ttccccaagt tcgaacagtt actctcccac atctcctcaa 5280
tattctccaa catctccaag ttattcgcct tcttcgccca aatattcacc aacttccccc 5340
aattattcgc caacatctcc atcattttct ggaggaagtc cacaatattc acccacatca 5400
ccgaaatact ctccaacctc gcccaattac actctgtcga gtccgcagca cactccaaca 5460
ggtagcagtc gatattcacc gtcaacttcg agttattctc ctaattcgcc caattattca 5520
ccgacgtctc cacaatactc catccacagt acaaaatatt cccctgcaag tcctacattc 5580
acacccacca gtcctagttt ctctcccgct tcacccgcat attcgcctca acctatgtat 5640
tcaccttctt ctcctaatta ttctcccact agtcccagtc aagacactga ctaa 5694
<210> 110
<211> 876
<212> DNA
<213> 玉米根萤叶甲属
<400> 110
atgtcgtcaa atattcaaaa ggcccagcag ttgatggcgg atgcagaaaa gaaagtaaca 60
tctcgaggtt tcttcggatc tctatttggg ggatcaagtc gtattgaaga tgcagtggaa 120
tgttacacaa gagctgcaaa cctttttaaa atggccaaga gctgggatgc tgccggtaaa 180
gccttttgtg aggctgctaa tttgcattcc agaactggtg ctcgtcatga cgctgccact 240
aattatatag atgctgcaaa ttgttacaaa aaagccgatg tatttgaggc tgtaaactgc 300
tttataaaag ctatagacat ttataccgaa atgggtcgct ttacaatggc tgcaaaacac 360
catcagacta ttgcagaaat gtatgagact gatgctgtgg acatcgaaag ggctgttcaa 420
cactatgaac aggcggctga ttacttcaga ggagaagaaa gcaatgcttc cgccaataag 480
tgtcttctta aagtggctca atatgcagcc caacttgaaa actatgaaaa agcagtggga 540
atttatcaag aagtggctta tgcagctctg gaaagctctc ttttaaaata cagtgcaaag 600
gaatacttat tcagagctgc cctttgtcac ctttgtgttg atgtactcaa tgcacaacat 660
gctatagaaa gctatatttc aaggtatccc gcatttcaag attcccgtga atacaaactt 720
ttgaaaaccc tcatagaaaa catcgaagag caaaacgtag atggatatac agaagccgtc 780
aaagattacg attcaatttc tcgtcttgat cagtggtata ctacaattct tttacgtatt 840
aagaaacaag taagcgaaag ccctgactta cgttaa 876
<210> 111
<211> 609
<212> DNA
<213> 玉米根萤叶甲属
<400> 111
atgaatcccg agtatgatta tttattcaaa cttctgctga ttggagattc aggagtagga 60
aaatcttgtc ttctactgag atttgcagat gatacctaca cagaaagcta tattagtacc 120
attggcgtag attttaaaat caggacaatc gatttagatg gaaagacaat taaattgcaa 180
atttgggata cagcaggtca ggaaaggttt agaacgatta catcaagtta ttaccgagga 240
gcacatggta ttattgtagt gtacgattgc acagaccaag attcattcaa taacgttaaa 300
cagtggctcg aagaaatcga ccgttatgcg tgtgacaatg taaacaaatt actggtaggg 360
aataaaagcg atttgacaac taagaaagtt gtcgacttca ctacagccaa ggagtatgcc 420
gaccaattgg gtataccatt tttggaaacc tcagctaaga atgcaaccaa tgtagaacag 480
gcctttatga ctatggccgc tgaaataaaa aatagagtag gacctccatc ttctgcggta 540
gaccaaggaa ataaggttag gttcgatcaa agtcgcccag tcgaaacaac caaatccggt 600
tgctgctga 609
<210> 112
<211> 789
<212> DNA
<213> 玉米根萤叶甲属
<400> 112
atggcagacg ctgatgatct attagattat gaagatgagg aacagacaga acaaaccgca 60
actgaaacgg caactacaga ggtacagaaa aagggtgtca agggcacata tgtatcaata 120
cacagttctg ggtttagaga ttttctgtta aaaccagcaa ttctcagagc tatagtggac 180
tgcgggttcg aacatccttc agaagttcaa catgaatgta ttcctcaagc tgtcattggc 240
atggatattc tgtgccaagc taaatccggt atgggaaaaa cggctgtttt tgtattagct 300
acactccaag taatagatcc tacagaaaat gttgtatatg ttctcgtcat gtgccatacc 360
agagagttag ccttccagat aagcaaagag tacgaacgtt tcagtaaata tatgcccaat 420
attaaagtag gggtcttctt tggtggcttg cctatccaga aagatgagga aacgttaaaa 480
aataattgcc cgcatatcgt tgtgggtact ccaggaagaa ttttagcatt ggtcagatcg 540
aaaaaactta atctcaaaca tctaaagcat tttattttgg atgaatgtga taaaatgttg 600
gagttattag acatgagacg tgatgttcaa gaaatatatc gtaacactcc ccacgaaaaa 660
caagtcatga tgttcagtgc caccttaagt aaagaaatta gaccagtttg caagaaattt 720
atgcaagatg taattcaaaa ttcttataat acacaatttt gtaatgacgc acccactcgc 780
aatgtttga 789
<210> 113
<211> 738
<212> DNA
<213> 玉米根萤叶甲属
<400> 113
atgccggtca ttgatggtta taaagtactt tacattttat tacacagttt atatacaatt 60
tttgaaaata tttggaggac tcttttattt atttatcaaa attgtataag ggttataaac 120
cctgaatcta cattcgatga tgctgaccag ttaaagaaaa gactgtctag actaacaaaa 180
aagcctcaac atttaactat cattattggt gtggaagaat attcattggt agatttggct 240
aacctcgtat attggtgttt aggtcttaat attccgtacg ttagtttcta tgattataaa 300
ggtaatttaa aaaagcatga agagaagttg caacaaattg tagaatccag aaaatcagag 360
aatatcaaca taatttggca cacccatgca gaacaaaggc ataaaaatgg atttttgggt 420
ccaaaaatcc acgtaaaagt gttaacacac gcggacggaa agcaaagtat agtaaatgtt 480
actaaaaaat tagctctaaa taaagaaaaa gacattagta aagaaaaaat tagtgaatta 540
ctattaaggc agtatgaatt tccagatcca gaaatggcta ttatttgtgg aaagaaactg 600
aacatttata attatcctcc ttggcagtta agactcacag aattctttaa agtcaacaaa 660
gtcaacaaca tcacattccc agtgtttgtg gaaaaattgg aaaagtacag caaatgtgaa 720
cagagggtgg gaaaataa 738
<210> 114
<211> 480
<212> DNA
<213> 玉米根萤叶甲属
<400> 114
atgaccaact ctaaaggtta ccgccgagga accagggatc tatttgcccg caagtttaaa 60
aaacgtggtg taattccact ttccacatat ttgagagtct acaaagttgg agatattgta 120
gatatcaagg gtaatggtgc agttcaaaag ggtatgcccc acaaagtgta ccatggtaag 180
acaggacgtg ttttcaatgt tactgcacat gcattaggtg taattgtaaa caaaagggtt 240
cgaggaagaa tcatccccaa aagaatcaat ctccgtattg aacatgtaaa ccactccaag 300
tgtcgtcaag acttcttgca aagagtaaaa tccaacgaaa agctacgtaa agaagctaaa 360
gaaaagaaca ttaaagtaga acttaggaga caacctgccc aacctaggcc agcacatatt 420
gttagcggaa aggttccagc acaggtgctt gctcctatcc catatgaatt cattgcttag 480
<210> 115
<211> 537
<212> DNA
<213> 玉米根萤叶甲属
<400> 115
atggaaggaa tactactgga accaacattg tataccataa aaggtattgc tatattggac 60
tatgatggta atagagtgct ggctaaatac tacgataaag atatatttcc tacagcaaaa 120
gagcagaaag cttttgagaa aaatttgttc aataaaactc atagggcaga cgcagaaatt 180
atcatgttgg atggtttaac ttgtgtgtat agaagtaatg tagatttatt cttttatgtt 240
atgggcagtt cacatgaaaa tgagctaatt ttaatgagtg ttttaaattg cttgtatgac 300
tcagtaagtc aaatattgaa gaaaaatatg caaaaacgag ctgtcttgga atcactagat 360
attgttatgc tggctatgga tgaaattgtt gatggaggaa taattataga ttctgattca 420
agttcagtag tatctagaat agcattaagg actgatgata ttccattagg agaacaaact 480
gtagctcagg tattccaaac ggccaaagaa cagctgaaat ggtcattgct gaaataa 537
<210> 116
<211> 450
<212> DNA
<213> 玉米根萤叶甲属
<400> 116
atggctgacc aactcaccga agaacaaatt gctgaattca aagaagcttt ctcactattc 60
gataaagatg gtgatggtac aattacgact aaagaattag gaacagtaat gagatctcta 120
ggacaaaatc caacagaggc tgaattacag gatatgatca atgaagtaga tgccgatggt 180
aacggcacga tcgatttccc agaattttta acgatgatgg cacgtaaaat gaaagatacc 240
gatagtgagg aagaaattcg tgaagcattc cgagtgttcg acaaagacgg caatggtttc 300
atctcagcag cagaattgcg ccacgtcatg accaacttgg gtgaaaaatt gacagacgaa 360
gaagtcgatg aaatgattcg ggaggccgat atcgatggtg atggtcaagt caattacgaa 420
gagttcgtca ccatgatgac ttcaaagtga 450
<210> 117
<211> 2013
<212> DNA
<213> 玉米根萤叶甲属
<400> 117
atgatgcaag caaacaatcg agtcccacct ataaagttgg aaaacgatat agatctttac 60
gccgatgata tcgaggattt cgcacaagat gactttggtg gtgaaaatgt tgatctatat 120
gacgatgtaa tatccgctcc tcctggaaat aatgacaacc caggtgattc aaatcatcat 180
gctcctcctg gtgctggtga agatggtgga ggtaattttg ttgggtcagg aggagcaccc 240
aataatataa attcttctgg aagaagacat cagctgtatg ttggaaatct gacttggtgg 300
acaactgatc aagatataga aaatgcagtg catgatatag gggtaaccga cttccatgaa 360
gttaagtttt ttgaacacag agcaaatggt caatccaagg gattctgtgt catatctttg 420
ggatctgagg gaagcatgag actctgcctg gaactcctat ctaaaaaaga gatcaatggc 480
caaaatcccc ttgttaccct tcccacaaaa caagctctta gtaactttga aagtcagtct 540
aaaacacgcc cttctcctac taataattct aactcacgtc ctccccatcc taataataat 600
gttcattcag gtcctatgca gaattatgga ggtagaatgc ctatgaaccc ttccatgcgt 660
cccatgcccc caggtatgca aggtgctcca agaatgcagg gtccacctgg atttaatgga 720
ccaccaaaca tgaatcagca accccccagg ttccaaggta atccacaatg gaatggacct 780
agacctaatg gtcctgggcc caatatggga atgagaccca tggggccacc tcatggacaa 840
caagggcccc caagaccacc aatgcaggga ccaccgcagc aaggtcctcc aagaggaatg 900
ccgccacaag gtccaccgca gatgcgtcca gaatggaatc gaccaccaat gcaacaaggg 960
taccctcaag gcccgccgca tatgcaagga cctaacatgg gtccaagagg tccaccccaa 1020
atgggaccac ccggggcgcc tcaacagcaa ggaccagctc cgcacgtaaa tccagcattc 1080
tttcaacaag gaggaggacc accgccccca atgcaacaca tgcctggacc agggcccgtc 1140
atgcctcctc aaggaccccc gcaaggtcca ccacacggac ccgttggacc tccacacggc 1200
ccaccattgg gtccagcgaa tgttccgcct catggaccac ctcacggata tggtccacct 1260
gcagcgatgc cacagccgcc atacggtggc ccacctccag accaccgcgc tgagattcct 1320
cagttaacag agcaagagtt tgaggatata atgtcccgga atagaacagt ttccagttcg 1380
gcgattgggc gggccgtatc cgacgccgca gctggagaat ttgcaagcgc cattgagact 1440
ttggttactg ctatttcact catcaaacaa tccaaagtgg ctaacgacga tcgttgcaag 1500
atccttataa gttcgctgca agatactttg cgtggtgtcg aagacaaaag ctacagctcc 1560
agccgcagag accggtcaag atccagggac agatcacata gaagaactag aagagaacga 1620
tcctcgtcac ggtacagaga cagaagcaga gagagggagc gtgaacgcga tagagatcgt 1680
gatcgtgaac gtgacagata ttatgataga tacagcgaaa gagaaagaga ccgagatcgt 1740
tcaagaagca gagaaagaac agaaagggat agagaacgag attatagaga ccgggaaccc 1800
gaagagacag ataaagaaaa atctaaagta tccagagtct caagatcaag aaacaaatct 1860
ccggaacctg tcgaacctag cagcgaggta ccgaaatcat cccgctatta tgaggatagg 1920
tatcgggaac gagagagaga aggtcgacga gagagcgatc gcgaaagaga aagagataga 1980
agaggggaag acagccatag gtctcgacac tag 2013
<210> 118
<211> 765
<212> DNA
<213> 玉米根萤叶甲属
<400> 118
atgagttcta ttggaactgg gtacgattta tcagcttccc aattctctcc tgatggaaga 60
gtatttcaag ttgaatatgc aatgaaagca gttgaaaata gtggcaccgt aataggcctc 120
cgaggtacag atggcattgt attggctgct gaaaagctca ttatgtcaaa attgcatgaa 180
ccaagtacaa ataaacgaat tttcaacatt gataaacaca taggaatggc attttcaggc 240
ttaatagctg atgcaaggca aatcgttgag attgctagaa aagaagcatc aaattataga 300
catcaatatg gttcaaatat tcctcttaaa tacctaaatg atagagtaag catgtacatg 360
catgcataca ctttatacag tgctgttaga ccatttggtt gcagtgtcat cttggccagt 420
tatgaagata gtgacccatc tatgtatctg attgatccat ctggagttag ctatggatac 480
tttggatgtg ctacaggtaa agcaaaacag tctgcaaaga ctgaaataga aaaattgaag 540
atggggaatc taacatgcaa agaacttgtt aaagaagcag ccaaaatcat ttatttggtc 600
catgatgagc tgaaggataa gaattttgaa ctggaacttt catgggtatg caaagatacg 660
aatggtttac ataccaaagt gcctgaatca gtgtttgctg atgcagaaaa agctgccaaa 720
caagcaatgg aagcagattc agaatcagat acagaagata tgtaa 765
<210> 119
<211> 744
<212> DNA
<213> 玉米根萤叶甲属
<400> 119
atggcttcaa aagacagatt gatgattttt ccatctagag gagcccaaat gatgatgaaa 60
tccaggctaa agggagccca aaagggacat agtttattaa agaagaaagc tgatgcttta 120
caaatgagat ttagaatgat tttgaacaaa attattgaga ccaaaactct catgggtgaa 180
gtaatgaaag aagctgcctt ttctttagct gaagcaaagt ttgcaactgg tgacttcaat 240
caagttgttc ttcaaaatgt caccaaggct caaataaaaa taagaactaa gaaagacaac 300
gttgctggtg ttactttacc agtgtttgaa tgctaccaag atggtacaga tacatatgag 360
ttggctggtt tggctagggg aggtcaacaa ttgacaaaac tcaagaagaa ttatcaaagt 420
gctgttaaac tgttggttga attagcctct ttgcaaactt cttttgtaac tcttgatgat 480
gtaatcaaaa taacaaacag aagagtcaat gccattgaac atgttatcat tccaagaata 540
gagcgtactt tggcttacat catatccgaa ctggacgagt tagaaagaga ggagttctat 600
agattaaaga agatccagga caaaaagaag atcagcagag caaaggccga gaaacaaaaa 660
caagctcttc tccaagctgg gctacttaaa gagtcccagg caaacatgct tttggatgag 720
ggcgatgaag atctactttt ctag 744
<210> 120
<211> 4818
<212> DNA
<213> 玉米根萤叶甲属
<400> 120
atggaggcgg ctcccaagtt accgatgctc tcgttcgagt taaatacttg tacagagaac 60
gtccactttg gcccccagtt aaaacagtat attgctgctt tttatggtga agatccagaa 120
tcctacatta cagaaatcag caatcttgaa tccttaagat cagctgcagt tcgaccatca 180
acggatgtaa atggtgtaca actgttgaaa aagtatttct gtcagcttcg ttttctcaaa 240
tctaggtttc ccatggaaga gaatcaagat gctgcagttc tattttcatg gaaaaataat 300
gaattagaca taacttcaac atccagtgat atcagatatg aattaatggt aataatgtat 360
aacattggag ccttacacac ttttcttgga gccaacgact caagaaacaa tccggatggt 420
atgaaaatgg catgtactca ttttcaatgt gctgcatggg cttttcaaaa cgtaaaagaa 480
aagtaccacc aattcatatc aaacatctca ttggtagaac tggttcattt ttttcaacaa 540
gtctgtttag ctcaggctca ggagtgtata ttagagaaga gcatgtttga caataggaaa 600
cctaccatca ttgcaaaagt tgctatccaa gtctacagtt attacagaca gtctttacgt 660
gtcttggaat cagtaaatga agcctacttt agggataaaa cctacaagga gtggatgaaa 720
tatcttcaat tcaagctgac ctactacaaa tgcatctcgt tcctattcca agggcaacaa 780
gctgaggaac aacagaaaat gggagaaagg gttgcattct atcaagctgc atgtgaacag 840
ctggacgagg caaagaaaat tgctgctaca ttaaaaaacc aacaccacca gcaagaaata 900
aatgagggac tagcattcac tactgatgtg gttgaaggta aaagaaaagc agctaaaaat 960
gaaaatgagt tcatctacca tgaatcagtg cctgataaag accaattgcc agaggttaag 1020
ggtgcttcat tagtcaaagg aataccattc agtataaatg atatagaagt ttcaggacca 1080
gatattttct cccgattggt cccaatggag gcacacgaag cagcttcctt gtacagcgag 1140
aagaaagctc agagattaag acagatcggg gaacttattg aaaataaaga tcaaacattg 1200
gctgaattta tgtcgtcaat gcagctagat ctattgacca agatgcacca ggctactgga 1260
ataccgcagg agttgattga tagagcagcg gctctatctg ctaaacctaa cgccattcaa 1320
gatcttataa gtgctatggg aaagctatct aatatatacc aagacgttga agcaagtttg 1380
aatgagattg attctttatt aaaggccgaa gaacaaagtg aacaaaagta ccaagaaacg 1440
attggtaaaa gaccaccgag cattttagct acagatttaa ctagggaagc ggcaaaatac 1500
agggaggctc atactaaagc gaacgactca aaccaaactt tacacagggc gatgatggct 1560
cacgtggcta atctgaaaat actccaacaa ccgctaaagc agctgcaaca tcagctgccc 1620
tttgtcgagt ttccaaatcc aaatatcgac gaaaaatctt tgaaagatct ggaagcgcta 1680
gttgcaaaag tagacgaaat gagaacccaa agagccatgc tatgggctca acttcgagaa 1740
tctattcacc aagacgatat tacaagttcc cttgtaacga aacaaccaaa tcagtcgctg 1800
gaacagctgt tccagcaaga acttcaaaag catcaaaatc tgatttcgtt gattgaacaa 1860
aacacctcgg cacaagaaaa cattaagagc gccttagtcg attcttacgc ttacgctgta 1920
aattcaagaa aatacatcca agatatactc caaaagagaa ccacaaccat aacgtcactg 1980
atagcatcgt tcgactctta cgaagactta ttggcaaaag ctaacaaagg gatagagttt 2040
tactcaaaac ttgaaacgaa cgtatccaag ttactgcaaa gaataaggag tacctgcaaa 2100
gttcaacaag aagagcgaga tcagatgatg tcgactgcgc aagtgcctca atgggagagt 2160
catacgtcac ttgccgctcc taaactgaaa gattacttgg actccaggaa gaagagtgct 2220
gcgtattcgg agccgagtgt tcaaccacaa cagccaactt taagttactc agctgctatg 2280
gatctgcctc ctggtattag gccgactcca gttggatcag aaataacgga tgtaccgaaa 2340
aatattcaag gtgaaccaca aggttatatt ccatataatt accaacaacc ttctgttcct 2400
gcctcacaga atattgatga agagactatt aaaaaaatga acgcattgat gccaggtgct 2460
aagacgtcag tgcctagtca gtacggatac agcaactaca ttccaccaac ataccctcaa 2520
agtgcgtacc aaccaggtaa tcagtcttac ggaaaagaaa ctccagatat taactcaccg 2580
tacgacccta ccaaggcgtt cacggctact actaacgctt atcgttcggt gcagagctcc 2640
tcaactcaag gatacgtacc gtacgcagaa tctaacgttt cgaatgttga cagagttgga 2700
tatcctagca ggtatcagta ccaacaagta cctgagatag ctactactcc agctgatccc 2760
aatattaatg cgtactaccc acatgggtac tcaccgagcc agaatttacc gaatgctaat 2820
actcaacata ttaccggcca actgaagtac cattcggtgg agtacgcttc ttctgtgccg 2880
aacaacatca attataacag ctctacctac tcgtcgccgc tttctaatat gtctagtacc 2940
aattcctcaa atcctagtaa cttgaataat tcttacgagt actactatga cccgaatacc 3000
agtagtggtg cagtaccgaa tgcttcaaag cctcaacagt cgagcgccag ctctgcaaac 3060
ccgagtaccg ctatgaacaa ctacaattat tactacaata caagtaccag cggtagtgta 3120
gcagcggata cttcaaaaat acaacaacaa caacagtacc caggtactca gatgagtcaa 3180
gcgcagtact atcccgccaa tgccagttat tactcaacca gtacttacaa taccaacgtc 3240
caaggtggta ccaatccctc gtacgcaact ggacaaacat ataatcaagt gacaccagtg 3300
acctctcaaa atgtttctca aaattacaac tttaaccaag ttggttctgg agcaggacac 3360
cagcatcagt actactcgtc cgctaacgcc gcagtaccat cccaacaagc tgtaaataac 3420
agttcattac caaactacgg atacgatcag tattacggca acaactataa ttccagtcaa 3480
ccgagtacct acagcgcaaa ccaagcacct cctgcagcac aagctgctcc aagtaatatt 3540
cctgctgcca ccaaatcctc ctctaatgtg gatctgctca gtggcttgga cttcagcata 3600
agccaagctc ctctagtgcc tcaacaaaac attacgataa aaccccaaga aaaggaaaca 3660
aaaccaccgg ctgtttcttc tgaaaccaaa aaccaagatc caacaccagt aaccacgccc 3720
aaacaaccca ctggaccaga agtaaagcgc ttgtacgtca aaatcctgcc gagcaaaccc 3780
ttaaacaacg atgatgtgaa gaaattgttc ggccaagagc tggacaggta tgagaagttc 3840
gtggagacct tgacccacaa aactttgagc ggtccgacca ctctggatat taaatggaag 3900
gagatccaag accagcagga ttgcgagccg cagaagaaga tcatttccgt cgctagatgt 3960
tatcctatga agaataggtt cccggatatc ttgccttacg acttttccag ggtggagttg 4020
tgcgatagta aagatgatta tatcaacgct tcatacatta aggatatctc gccatatgct 4080
ccgtcattta ttgttacaca agtgccgttg tcttcaactg ttggtgatat gtggacgatg 4140
attagagaac aacaggtcga actgatcctc tgtttggtaa acgacaatga gatcggtgaa 4200
gatatttact ggcccaaaga aaaaggcagt agtcttaaca tacttaacat ggtcataacg 4260
ttgcaaaacg ttatagttaa gtctcattgg actgaaagac tgatagcgat aaacttacct 4320
gaaaaacggg agtcccgtgt gataatgcat ctacaattta catcgtggcc tggcagcttg 4380
tttccaacaa atcctgaacc gttcgtcagc tacaccttgg aatccatcaa cctataccaa 4440
caacagaaga ccaacaccca tccggtggtg gtccattgtt catctggcat aggaagaagc 4500
ggcctgctct gtttactgac agctgctatg ttcgatgctg ccaacaatgc taactcgata 4560
ccagatctta cagctttgag tatcaagttg tccaattgca ggaagaatat tctcagagat 4620
cgagagcatt tgaagtttgg ttacgaaagt tttttggcgt atattaggca tatagtttgt 4680
gaagataaag ccagaaagaa actgaacgag atccagccca aggttaagga ggaaccactg 4740
gaaccacctg tcatagttcc agaaccaaat atagatcctt taagtacttt agacccattt 4800
tgggctagta aaagataa 4818
<210> 121
<211> 478
<212> RNA
<213> 玉米根萤叶甲属
<400> 121
gguuucauga ccccugagag aaagaagaaa cuuagguuac uguugagaaa gaaagccgcc 60
gaagaauuaa agaaagaaca agaacgcaaa gcagccgaaa ggaggcguau cauugaagaa 120
aggugcggua aacccaaacu ugucgaugac gcaaaugaag gcccauuaaa acaaguaugu 180
gagggauauc acagacguau uguagaccua gaaaauaaga aauuugaccu cgaaaaagaa 240
guggaauuca gagauuuuca gaucuccgaa uugaacagcc aaguaaacga ccuuagaggc 300
aaauucguca aaccaaccuu gaagaaggua uccaaauacg aaaacaaauu cgccaaacuu 360
caaaagaagg cagcugaauu uaacuuccgu aaccaacuca aaguugucaa gaagaaagaa 420
uucaccuuag aagaagaaga caaagaaaag aaaccagacu ggucaaagaa gggagacg 478
<210> 122
<211> 529
<212> RNA
<213> 玉米根萤叶甲属
<400> 122
agaaguugcc gcuuuagucg uagacaaugg auccgguaug ugcaaagcug guuuugcugg 60
ggaugaugca ccucgugcug uauucccuuc aauuguugga cgcccaagac aucagggugu 120
gaugguagga augggacaaa aagauuccua uguaggugau gaagcucaaa guaaaagagg 180
uauccuuacc uuaaaauacc ccaucgagca cggaauaguc acaaacuggg augauaugga 240
gaaaauuugg caucauacau ucuacaauga acucagagua gccccagaag aacacccugu 300
ucuguugaca gaagcuccuc ucaaccccaa ggccaacagg gaaaagauga cacaaauaau 360
guuugaaacu uucaacaccc cagccaugua uguugccauc caggcuguac ucuccuugua 420
ugcaucuggu cguacaacug guauuguguu ggauucuggu gaugguguau cccacacugu 480
cccaaucuau gaagguuaug cucuuccuca ugcaauccuu cguuuggac 529
<210> 123
<211> 592
<212> RNA
<213> 玉米根萤叶甲属
<400> 123
accggccuuu guaugucuug ggauaugugc cuaaagacga uagauuauac cucguagaua 60
aagaguugcg cguaguaagc uaccaauuac uucuuucugu ucuugaauau caaacugccg 120
ucaugagaag agacuuucca acagcagaca gaguacuucc guccauuccu aaggagcaca 180
gaacgagagu ggcacauuuc uuagaaaagc aaggcuucaa acagcaagcu uuggccguaa 240
guacagaucc agagcacaga uucgagcugg caguagcauu agaggaucuu aauauagcca 300
aaacucuagc ucaagaagcg aacaguccgc aaaaguggaa ucaacuagca gaauuggcag 360
cugcuacuaa uaauguaagc guagccaagg aauguaugca aaaagcgcaa gauuauggag 420
gcuuguugcu ucuugcuacg agcuccggug augaaaauuu aguccguacu cuaggagaaa 480
cgacacaagc ugaaagcaaa cauaacuuag cauuuuuguc acacuuguua guaggugauu 540
uaaacaaaug ucuagacauu cuuauuaaua ccgguagauu gccagaagcu gc 592
<210> 124
<211> 594
<212> RNA
<213> 玉米根萤叶甲属
<400> 124
gaaagcaguu ggaagauggc cguacucucu cagacuacaa cauucaaaaa gagucuaccc 60
uccauuuggu acuucgucuu agaggaggua ugcagauuuu uguuaaaacu uuaacuggaa 120
agaccaucac ccuugaagua gaaccuucug auaccaucga aaaugucaaa gccaaaauuc 180
aagacaaaga agguauucca ccagaucaac aaagauuaau cuuugccgga aagcaauugg 240
aagauggucg uacacucuca gacuacaaca uucaaaagga aucuacccuc cauuugguac 300
uucgucuuag aggagguaug caaaucuuug uaaaaacacu cacugguaag accaucaccc 360
ucgagguuga accaucagau accaucgaga augucaaagc uaaaauucaa gacaaagaag 420
guauuccacc agaucaacag agauuaaucu ucgcuggaaa gcaguuggaa gauggccgua 480
cucucucaga cuacaauauu cagaaagagu cuacccucca uuugguacuu cgucuuagag 540
gagguaugca aaucuuugua aaaacucuca cugguaagac caucacccuc gagg 594
<210> 125
<211> 547
<212> RNA
<213> 玉米根萤叶甲属
<400> 125
uaccccauug aacacggaau uaucacuaac ugggacgaua uggaaaagau cuggcaucac 60
accuucuaca augaacuuag aguagccccc gaagaacauc ccauucuuuu gacugaagcu 120
ccacuuaacc caaaagccaa cagagaaaag augacucaaa ucauguuuga aacuuucaau 180
accccugcca uguauguugc cauucaagcu guauugucuc uguacgcuuc cggucguacc 240
acugguauug uacuugauuc uggagauggu guaucccaca caguacccau cuaugaaggu 300
uacgcucucc cacacgccau cuugcguuug gacuuggccg guagagacuu gacugacuac 360
cuuaugaaga ucuuaaccga aagagguuac ucuuucacca ccacagcuga aagagaaaua 420
guucgugaca ucaaggaaaa auugugcuau guagcuuugg acuucgaaca ggaaauggcc 480
acagcagcca gcuccaccuc cuuagaaaag aguuaugaac uuccugacgg ucaagucauc 540
accauug 547
<210> 126
<211> 592
<212> RNA
<213> 玉米根萤叶甲属
<400> 126
aggaaaggaa ggacccaaga ccaaacaacc aucgagauac auccguuuua ucuacaaucg 60
cgucauauug gaauguccuu cuguaagagc ugcugcaguc uccgccaugg cacaauucgg 120
agccucuugu cccgauuugu uagaaaauau ccaaauauua cuuucgaggu gucagaugga 180
uucagacgau gaaguuaggg acagagcuac auauuauagu aauauacuua acaaaaauga 240
uaaaaguuua uacaacaauu acauuuugga uucuuugcag guuucaauuc cuucacuaga 300
aagaucgcuu agagaauaca uucaaaaucc aacugacgaa ccauuugaca uuaaguccgu 360
accuguagca gcagugccaa cagcagaaga acgagaaguu aaaaacaaau cugaaggacu 420
gcuagucucu caagguccag uccgaccucc uccggugucu agagaagaaa acuucgccga 480
aaaacuuagu aacguuccgg guauacaaca guuaggaccu uuguucaaaa cuuccgacgu 540
cguugaacuc acugaaucug aaacagagua uuuuguccgc uguaucaagc ac 592
<210> 127
<211> 500
<212> RNA
<213> 玉米根萤叶甲属
<400> 127
uacuaggcga aagugccguc gguaagucga guuugguacu gagguucguc aaaggacagu 60
uccacgaaua ccaggagagu accauaggag cagcuuuccu uacacaaacc auaugccucg 120
acgauacaac uguuaaauuu gaaauuuggg acacagcggg ucaagaaagg uaccacaguu 180
uagcuccuau guacuauagg ggcgcacagg cagcuauagu cgucuacgac auaaccaauc 240
aagacacauu cggcagggcg aaaacguggg ugaaggaacu ucaaaggcag gccaguccga 300
cgaucgugau agcuuuggcc ggcaacaagc aagauuuggc caacaaacgu augguagaau 360
acgaagaggc gcagacguau gcugacgaaa acggcuuacu uuuuauggaa acuuccgcaa 420
agacggcaau gaacgucaac gauauauuuu uagcaauagc uaagaaacug cccaagaaug 480
aacaaaccac aggucaaggc 500
<210> 128
<211> 189
<212> RNA
<213> 玉米根萤叶甲属
<400> 128
cgaugagguu gaaggaagga gaaaaauuuu gauggggcga aaaagcauua ccaggacaua 60
ucuucgugga aaugcuguuc cugcguaugu gauaauaauc cuuguaggaa uuggucaaau 120
cauccuggga gggauauugu acguugcauu gaggaagaag aucauugcug caccuguaac 180
ggcaucaua 189
<210> 129
<211> 393
<212> RNA
<213> 玉米根萤叶甲属
<400> 129
ugaggucgag cccucagaua cuaucgaaaa ugugaaagcu aaaauccagg auaaagaagg 60
aauuccccca gaccagcaac gucucaucuu cgcuggaaaa caacucgaag auggucguac 120
cuugucugac uauaauauuc aaaaagaauc aacccuucac uugguguuga gauugagagg 180
aggugcuaag aaacguaaga agaagaauua cuccaccccc aagaaaauca agcacaagaa 240
gaagaagguu aaguuagcug uauugaaauu uuauaagguu gacgaaaaug guaaaaucca 300
ccgauugaga cgugaaugcc ccgcugaaca auguggagcu ggugucuuca uggcagccau 360
ggaagacagg cauuacugug gcaagugcgg uua 393
<210> 130
<211> 524
<212> RNA
<213> 玉米根萤叶甲属
<400> 130
ucuuugccuu uggcuacgau ucgaggaggg cauucaagaa uggcuccaga aauuggauuc 60
agaacaauuu uacaucggag uauauguacu uauagucgcu ucacugaucg ucaugauugu 120
guccuuuaua ggauguauua gugcccugca ggagaguacc auggcccuuu uaguguacau 180
cggcacccaa gugcucaguu uuauauucgg uuuauccggu ucggcgguuc uucuggauaa 240
cagcgccaga gauucccacu uccaaccgag gauccgagag aguaugcgac gucuuaucau 300
gaaugcucau cacgaccaau ccagacaaac acuagccaug auucaggaaa auguugguug 360
cugcggagcu gauggcgcaa cagacuaccu cucucuucag cagccccuuc caagucagug 420
cagagacacc guuacuggaa acccauucuu ccacggaugu guagaugaac ucaccugguu 480
cuucgaagaa aaaugugguu ggauagcagg uuuagcuaug gcga 524
<210> 131
<211> 267
<212> RNA
<213> 玉米根萤叶甲属
<400> 131
ucgugcuggu uuacaauuuc cuguaggucg uauucaucgu uuauugagaa aaggaaauua 60
ugccgaaaga guuggugcug gagcuccugu auacuuggca gcuguuaugg aauauuuagc 120
ugcugaaguu uuggaauugg caggaaaugc agcuagagau aacaaaaaga cccguauaau 180
uccuagacau uuacaauugg ccauaagaaa ugacgaggaa uugaacaaau uacugucagg 240
aguuaccauc gcccaaggug gaguauu 267
<210> 132
<211> 558
<212> RNA
<213> 玉米根萤叶甲属
<400> 132
gugcaugaag uuggauggug uagaucugcc cccaccaauu agcuucgaca uugcggaaga 60
gcaaccguua ccaccuugcc aacagacguu cuuauguaau ggugauggag gauccauagu 120
gcgacaguuu cucgagcugu auuucguaau auaugauuca gauaauaggc agucccuucu 180
ucaggcauau cacgaaaaag ccacauuuuc aaugacaaug gccuacccgu acggcuauuc 240
caaagacagu aaaggaguau cgugguugaa uugguaugcc accgauaaua gaaauuuauu 300
acgaguucaa gauccagaca gaagaaacaa guuguuaaga cagggacaag uugcuguagu 360
uucguucuug caagauaugc cgcacacgaa gcacgauauu cacaguuuua caguagauuu 420
gacaguuuuu acaccccaga uguuauguuu gacaguggcu gguauguuua aagaauugaa 480
aaguggccac aaaguaccuc cuuuaagaua uuucuucaga acccuuguaa uuguaccugc 540
uggaucaggu uuuugcau 558
<210> 133
<211> 582
<212> RNA
<213> 玉米根萤叶甲属
<400> 133
gauucggagc ccuacaauga aaugcaacua aaaauggauu uagaaaaggg ugagguuaaa 60
guaaaaauaa gagcauuaga aaaaauaauu cacaugauuc uggcaggaga aagguugccg 120
aauggauuuc uaaugaccau cauaagaaac guuuuaccuu uacaagauca uuuggcaaaa 180
aaacuauuau ugauuuucug ggaaauaguu ccaaaaacaa auccagaagg uaaacuacua 240
caagagauga uuuugguaug ugaugccuau agaaaagauc ugcaacaccc aaaugaauuu 300
uugagagguu cuacacuucg cuucuugugc aaacugaagg aaccagaauu guuggaacca 360
uuaaugccca guauuagagc uuguuuggau cauaggcaca gcuaugugag gaggaaugcu 420
guacuggcaa uuuuuaccau uuacaaaaau uuugaagccc ucauuccaga ugcuccugaa 480
cugaucucca auuauuugga uggugagcaa gacaugucuu guaaaagaaa ugcguuuuua 540
augcuucuuc augcugacca agaaagggcg uugucguauu ug 582
<210> 134
<211> 458
<212> RNA
<213> 玉米根萤叶甲属
<400> 134
uuggcaaaaa ggaaaugagg auauugaugg uaggacucga ugcagcuggu aaaaccacaa 60
uuuuauauaa acuuaaauua ggagaaauug uaacaacuau uccaacaauu ggauuuaaug 120
uggagacugu agaauauaag aacauuaguu uuacaguaug ggauguaggu ggucaagaua 180
aaauuaggcc auuguggaga cacuauuucc aaaacacaca aggccuaauu uucguaguag 240
acaguaacga cagggaacgu aucacugagg cuaaagauga auuaaugcgu auguuggccg 300
aagaugaacu uagagaugcc guacuucuca uuuucgccaa caaacaagau uugcccaaug 360
caaugaacgc ugcagaaauc accgacaaac ucggucucca uucacuacgc aaccgcaacu 420
gguacauuca agcuaccugu gcaacuagcg gagauggu 458
<210> 135
<211> 592
<212> RNA
<213> 玉米根萤叶甲属
<400> 135
ggucgcgaaa gaacagaaag auuuuuugac cguaucugcc gauggaugcg uacaaguaac 60
aaaaccucuc gaccgagauc cgccuuucgg uagcccaaca cgacaagucu ucaucuaugc 120
ucgugauaau gauggaggca caaauucauu guuggccacu gcagaaauug aaauuauuuu 180
aauagauaua aacgauaaug cucccuuuuu aaauguuaca gaaauuguuu auuaugaaaa 240
ccaggaucca gguuuuauag guaaccuaag ugccgaugau uacgaugguc cugauaaugg 300
accuccguuu gcuuuucgau uaucagacac ugcuucagau aguauuagau cgaaauuuuc 360
cauuaucgga aaccagcuuu ucgcuuuaga aauguuugau agagaagagc aaaaauauua 420
ugacauugcc auugacauua cagauagugg aguaccucca cuaacaggaa cuaguauucu 480
uagaguuaua aucggagaug uaaaugauaa uccagcuaca gacggaaaca gcacgaucuu 540
uguguauaag uacgucaaug ggccagaaaa uuucauggaa aucggacgug ua 592
<210> 136
<211> 214
<212> RNA
<213> 玉米根萤叶甲属
<400> 136
gcauggggua ucauccaguu ggguuucaug gguguauucu auuacauugg ggcuguggcu 60
uuagcagaag auauuccaga gguugaguuu aagggcgauu uagacaaauu uuauagcgac 120
gucaacacgg guuucacaca gaaugcuuac aacugcugga uugcugcucu ccuauaccug 180
auaacauuag caguaucagc ucaccaauuc uggg 214
<210> 137
<211> 459
<212> RNA
<213> 玉米根萤叶甲属
<400> 137
uagaugccgc agguaaaacc acaaucuuau acaaauugaa gcuuggugaa aucguaacua 60
caauaccaac caucggcuuc aauguagaaa ccguugagua caagaauaua ucuuucacgg 120
uaugggaugu agguggccag acgagaauca gaaaacucug gagacacuau uucgccaaca 180
cugauggacu cauuuuugug guugauucca acgaccgaga ccguaucgcg gaagccgaag 240
aagaauugca caauauguua ggagaggacg auuuaagaga cugcauuuug uuaauauucg 300
ccaacaaaca agauuuaccg aacucgaugu ccacugcuga auugaccgau aagcuuaagu 360
ugcacacuuu gaagaauagg aggugguaca uacaagccac augugcuacu caagggaaug 420
guuuguacga aggacuagau ugguugucga augaauugg 459
<210> 138
<211> 536
<212> RNA
<213> 玉米根萤叶甲属
<400> 138
gcuacccuag cguccacugu aacacugauu uuugcccucu acuacugccu cacgggaaaa 60
ggagagcaag uuaguuuagc augguuauug uugaaugugu cuccccacau gugggcaggu 120
cuaggaauug gccuugcugu aucauuauca guuguaggag cugcugcagg aauucacacu 180
acaggaguca guaucguagg agcugguguu aaagccccca gaaucaaaac caaaaauuua 240
auuucuauua uuuucuguga agcuguggcu aucuaugggu uaauuauggc uauaguacuc 300
uguggaaguu ggaagaauuu cgauguagac cuauucaacc ucaaaacuca uaacuuugcu 360
caaaaccauu auggaucaca uguuauuuuu ggauccgguu uaacuguugg auuuguaaau 420
cuauuaugug gauuuugugu uggaguaguu gguucuggug cagccauuuc ugaugcagcc 480
aauucaucau uauucgucaa aauuuugauu auugagauuu uuggaagugc cauugg 536
<210> 139
<211> 547
<212> RNA
<213> 玉米根萤叶甲属
<400> 139
accuuauggg aaagcgugug gacuuuucug cacguacugu caucacacca gaucccaauu 60
uacguaucga ccaaguagga gugccuagaa guauugcuca aaacaugacg uuuccagaaa 120
ucgucacacc uuucaauuuu gacaaaaugu uggaauuggu acagagaggu aauucucagu 180
auccaggagc uaaguauauc aucagagaca auggagagag gauugauuua cguuuccacc 240
caaaaccguc agauuuacau uugcagugug guuauaaggu agaaagacac aucagagacg 300
gcgaucuagu aaucuucaac cgucaaccaa cccuccacaa gaugaguaug augggccaca 360
gagucaaagu cuuacccugg ucgacguucc guaugaaucu cucgugcacc ucucccuaca 420
acgccgauuu ugacggcgac gaaaugaacc uccaugugcc ccaaaguaug gaaacucgag 480
cugaagucga aaaccuccac aucacuccca ggcaaaucau uacuccgcaa gcuaaccaac 540
ccgucau 547
<210> 140
<211> 564
<212> RNA
<213> 玉米根萤叶甲属
<400> 140
cggaucucua uuugggggau caagucguau ugaagaugca guggaauguu acacaagagc 60
ugcaaaccuu uuuaaaaugg ccaagagcug ggaugcugcc gguaaagccu uuugugaggc 120
ugcuaauuug cauuccagaa cuggugcucg ucaugacgcu gccacuaauu auauagaugc 180
ugcaaauugu uacaaaaaag ccgauguauu ugaggcugua aacugcuuua uaaaagcuau 240
agacauuuau accgaaaugg gucgcuuuac aauggcugca aaacaccauc agacuauugc 300
agaaauguau gagacugaug cuguggacau cgaaagggcu guucaacacu augaacaggc 360
ggcugauuac uucagaggag aagaaagcaa ugcuuccgcc aauaaguguc uucuuaaagu 420
ggcucaauau gcagcccaac uugaaaacua ugaaaaagca gugggaauuu aucaagaagu 480
ggcuuaugca gcucuggaaa gcucucuuuu aaaauacagu gcaaaggaau acuuauucag 540
agcugcccuu ugucaccuuu gugu 564
<210> 141
<211> 564
<212> RNA
<213> 玉米根萤叶甲属
<400> 141
ugcugauugg agauucagga guaggaaaau cuugucuucu acugagauuu gcagaugaua 60
ccuacacaga aagcuauauu aguaccauug gcguagauuu uaaaaucagg acaaucgauu 120
uagauggaaa gacaauuaaa uugcaaauuu gggauacagc aggucaggaa agguuuagaa 180
cgauuacauc aaguuauuac cgaggagcac augguauuau uguaguguac gauugcacag 240
accaagauuc auucaauaac guuaaacagu ggcucgaaga aaucgaccgu uaugcgugug 300
acaauguaaa caaauuacug guagggaaua aaagcgauuu gacaacuaag aaaguugucg 360
acuucacuac agccaaggag uaugccgacc aauuggguau accauuuuug gaaaccucag 420
cuaagaaugc aaccaaugua gaacaggccu uuaugacuau ggccgcugaa auaaaaaaua 480
gaguaggacc uccaucuucu gcgguagacc aaggaaauaa gguuagguuc gaucaaaguc 540
gcccagucga aacaaccaaa uccg 564
<210> 142
<211> 502
<212> RNA
<213> 玉米根萤叶甲属
<400> 142
gagcuauagu ggacugcggg uucgaacauc cuucagaagu ucaacaugaa uguauuccuc 60
aagcugucau uggcauggau auucugugcc aagcuaaauc cgguauggga aaaacggcug 120
uuuuuguauu agcuacacuc caaguaauag auccuacaga aaauguugua uauguucucg 180
ucaugugcca uaccagagag uuagccuucc agauaagcaa agaguacgaa cguuucagua 240
aauauaugcc caauauuaaa guaggggucu ucuuuggugg cuugccuauc cagaaagaug 300
aggaaacguu aaaaaauaau ugcccgcaua ucguuguggg uacuccagga agaauuuuag 360
cauuggucag aucgaaaaaa cuuaaucuca aacaucuaaa gcauuuuauu uuggaugaau 420
gugauaaaau guuggaguua uuagacauga gacgugaugu ucaagaaaua uaucguaaca 480
cuccccacga aaaacaaguc au 502
<210> 143
<211> 505
<212> RNA
<213> 玉米根萤叶甲属
<400> 143
ugguagauuu ggcuaaccuc guauauuggu guuuaggucu uaauauuccg uacguuaguu 60
ucuaugauua uaaagguaau uuaaaaaagc augaagagaa guugcaacaa auuguagaau 120
ccagaaaauc agagaauauc aacauaauuu ggcacaccca ugcagaacaa aggcauaaaa 180
auggauuuuu ggguccaaaa auccacguaa aaguguuaac acacgcggac ggaaagcaaa 240
guauaguaaa uguuacuaaa aaauuagcuc uaaauaaaga aaaagacauu aguaaagaaa 300
aaauuaguga auuacuauua aggcaguaug aauuuccaga uccagaaaug gcuauuauuu 360
guggaaagaa acugaacauu uauaauuauc cuccuuggca guuaagacuc acagaauucu 420
uuaaagucaa caaagucaac aacaucacau ucccaguguu uguggaaaaa uuggaaaagu 480
acagcaaaug ugaacagagg guggg 505
<210> 144
<211> 410
<212> RNA
<213> 玉米根萤叶甲属
<400> 144
aaccagggau cuauuugccc gcaaguuuaa aaaacguggu guaauuccac uuuccacaua 60
uuugagaguc uacaaaguug gagauauugu agauaucaag gguaauggug caguucaaaa 120
ggguaugccc cacaaagugu accaugguaa gacaggacgu guuuucaaug uuacugcaca 180
ugcauuaggu guaauuguaa acaaaagggu ucgaggaaga aucaucccca aaagaaucaa 240
ucuccguauu gaacauguaa accacuccaa gugucgucaa gacuucuugc aaagaguaaa 300
auccaacgaa aagcuacgua aagaagcuaa agaaaagaac auuaaaguag aacuuaggag 360
acaaccugcc caaccuaggc cagcacauau uguuagcgga aagguuccag 410
<210> 145
<211> 467
<212> RNA
<213> 玉米根萤叶甲属
<400> 145
ugaugguaau agagugcugg cuaaauacua cgauaaagau auauuuccua cagcaaaaga 60
gcagaaagcu uuugagaaaa auuuguucaa uaaaacucau agggcagacg cagaaauuau 120
cauguuggau gguuuaacuu guguguauag aaguaaugua gauuuauucu uuuauguuau 180
gggcaguuca caugaaaaug agcuaauuuu aaugaguguu uuaaauugcu uguaugacuc 240
aguaagucaa auauugaaga aaaauaugca aaaacgagcu gucuuggaau cacuagauau 300
uguuaugcug gcuauggaug aaauuguuga uggaggaaua auuauagauu cugauucaag 360
uucaguagua ucuagaauag cauuaaggac ugaugauauu ccauuaggag aacaaacugu 420
agcucaggua uuccaaacgg ccaaagaaca gcugaaaugg ucauugc 467
<210> 146
<211> 368
<212> RNA
<213> 玉米根萤叶甲属
<400> 146
caccgaagaa caaauugcug aauucaaaga agcuuucuca cuauucgaua aagaugguga 60
ugguacaauu acgacuaaag aauuaggaac aguaaugaga ucucuaggac aaaauccaac 120
agaggcugaa uuacaggaua ugaucaauga aguagaugcc gaugguaacg gcacgaucga 180
uuucccagaa uuuuuaacga ugauggcacg uaaaaugaaa gauaccgaua gugaggaaga 240
aauucgugaa gcauuccgag uguucgacaa agacggcaau gguuucaucu cagcagcaga 300
auugcgccac gucaugacca acuuggguga aaaauugaca gacgaagaag ucgaugaaau 360
gauucggg 368
<210> 147
<211> 430
<212> RNA
<213> 玉米根萤叶甲属
<400> 147
uucgcacaag augacuuugg uggugaaaau guugaucuau augacgaugu aauauccgcu 60
ccuccuggaa auaaugacaa cccaggugau ucaaaucauc augcuccucc uggugcuggu 120
gaagauggug gagguaauuu uguuggguca ggaggagcac ccaauaauau aaauucuucu 180
ggaagaagac aucagcugua uguuggaaau cugacuuggu ggacaacuga ucaagauaua 240
gaaaaugcag ugcaugauau agggguaacc gacuuccaug aaguuaaguu uuuugaacac 300
agagcaaaug gucaauccaa gggauucugu gucauaucuu ugggaucuga gggaagcaug 360
agacucugcc uggaacuccu aucuaaaaaa gagaucaaug gccaaaaucc ccuuguuacc 420
cuucccacaa 430
<210> 148
<211> 505
<212> RNA
<213> 玉米根萤叶甲属
<400> 148
uaggaauggc auuuucaggc uuaauagcug augcaaggca aaucguugag auugcuagaa 60
aagaagcauc aaauuauaga caucaauaug guucaaauau uccucuuaaa uaccuaaaug 120
auagaguaag cauguacaug caugcauaca cuuuauacag ugcuguuaga ccauuugguu 180
gcagugucau cuuggccagu uaugaagaua gugacccauc uauguaucug auugauccau 240
cuggaguuag cuauggauac uuuggaugug cuacagguaa agcaaaacag ucugcaaaga 300
cugaaauaga aaaauugaag auggggaauc uaacaugcaa agaacuuguu aaagaagcag 360
ccaaaaucau uuauuugguc caugaugagc ugaaggauaa gaauuuugaa cuggaacuuu 420
cauggguaug caaagauacg aaugguuuac auaccaaagu gccugaauca guguuugcug 480
augcagaaaa agcugccaaa caagc 505
<210> 149
<211> 548
<212> RNA
<213> 玉米根萤叶甲属
<400> 149
uccaucuaga ggagcccaaa ugaugaugaa auccaggcua aagggagccc aaaagggaca 60
uaguuuauua aagaagaaag cugaugcuuu acaaaugaga uuuagaauga uuuugaacaa 120
aauuauugag accaaaacuc ucauggguga aguaaugaaa gaagcugccu uuucuuuagc 180
ugaagcaaag uuugcaacug gugacuucaa ucaaguuguu cuucaaaaug ucaccaaggc 240
ucaaauaaaa auaagaacua agaaagacaa cguugcuggu guuacuuuac caguguuuga 300
augcuaccaa gaugguacag auacauauga guuggcuggu uuggcuaggg gaggucaaca 360
auugacaaaa cucaagaaga auuaucaaag ugcuguuaaa cuguugguug aauuagccuc 420
uuugcaaacu ucuuuuguaa cucuugauga uguaaucaaa auaacaaaca gaagagucaa 480
ugccauugaa cauguuauca uuccaagaau agagcguacu uuggcuuaca ucauauccga 540
acuggacg 548
<210> 150
<211> 521
<212> RNA
<213> 玉米根萤叶甲属
<400> 150
gaucuggaag cgcuaguugc aaaaguagac gaaaugagaa cccaaagagc caugcuaugg 60
gcucaacuuc gagaaucuau ucaccaagac gauauuacaa guucccuugu aacgaaacaa 120
ccaaaucagu cgcuggaaca gcuguuccag caagaacuuc aaaagcauca aaaucugauu 180
ucguugauug aacaaaacac cucggcacaa gaaaacauua agagcgccuu agucgauucu 240
uacgcuuacg cuguaaauuc aagaaaauac auccaagaua uacuccaaaa gagaaccaca 300
accauaacgu cacugauagc aucguucgac ucuuacgaag acuuauuggc aaaagcuaac 360
aaagggauag aguuuuacuc aaaacuugaa acgaacguau ccaaguuacu gcaaagaaua 420
aggaguaccu gcaaaguuca acaagaagag cgagaucaga ugaugucgac ugcgcaagug 480
ccucaauggg agagucauac gucacuugcc gcuccuaaac u 521
<210> 151
<211> 633
<212> RNA
<213> 玉米根萤叶甲属
<400> 151
auggcggacg augagagaaa gaaacuggag gaggaaaaga agaggaaaca ggccgaaauu 60
gaacgcaaaa gggccgaggu cagggcucgu auggaagagg ccucaaaagc caagaaggcc 120
aagaaagguu ucaugacccc ugagagaaag aagaaacuua gguuacuguu gagaaagaaa 180
gccgccgaag aauuaaagaa agaacaagaa cgcaaagcag ccgaaaggag gcguaucauu 240
gaagaaaggu gcgguaaacc caaacuuguc gaugacgcaa augaaggccc auuaaaacaa 300
guaugugagg gauaucacag acguauugua gaccuagaaa auaagaaauu ugaccucgaa 360
aaagaagugg aauucagaga uuuucagauc uccgaauuga acagccaagu aaacgaccuu 420
agaggcaaau ucgucaaacc aaccuugaag aagguaucca aauacgaaaa caaauucgcc 480
aaacuucaaa agaaggcagc ugaauuuaac uuccguaacc aacucaaagu ugucaagaag 540
aaagaauuca ccuuagaaga agaagacaaa gaaaagaaac cagacugguc aaagaaggga 600
gacgaaaaga agguacaaga ggcugaagca uga 633
<210> 152
<211> 603
<212> RNA
<213> 玉米根萤叶甲属
<400> 152
augugugaag aagaaguugc cgcuuuaguc guagacaaug gauccgguau gugcaaagcu 60
gguuuugcug gggaugaugc accucgugcu guauucccuu caauuguugg acgcccaaga 120
caucagggug ugaugguagg aaugggacaa aaagauuccu auguagguga ugaagcucaa 180
aguaaaagag guauccuuac cuuaaaauac cccaucgagc acggaauagu cacaaacugg 240
gaugauaugg agaaaauuug gcaucauaca uucuacaaug aacucagagu agccccagaa 300
gaacacccug uucuguugac agaagcuccu cucaacccca aggccaacag ggaaaagaug 360
acacaaauaa uguuugaaac uuucaacacc ccagccaugu auguugccau ccaggcugua 420
cucuccuugu augcaucugg ucguacaacu gguauugugu uggauucugg ugauggugua 480
ucccacacug ucccaaucua ugaagguuau gcucuuccuc augcaauccu ucguuuggac 540
uuagcuggua gagacuugac ugauuaccuc augaaaauuu ugacugaacg uggcuacucu 600
uuc 603
<210> 153
<211> 2742
<212> RNA
<213> 玉米根萤叶甲属
<400> 153
augccacuuc gauuagauau aaaaagaaag cuaacagcuc gcucagaccg gguaaaaugu 60
guggaucuuc acccuacaga accuuggaug cuguguucuc uuuacagcgg aaauauaaac 120
guuuggaaca ccgaaaauca gcaacugguu aagacuuuug aaguauguga uguaccuguu 180
cggacagcua aguuuuugcc caggaagaac uggauaguca gugggucuga ugauaugcag 240
auucgaguuu ucaauuacaa uaccuuagau cggguacauu cuuuugaggc ucauucggau 300
uaugugagau guauugucgu acacccuaca caaccuuaua uauuaacaag uagugaugau 360
augcuuauca agcuuuggaa uugggaaaaa gcaugggcuu gucagcaagu uuucgaagga 420
cacacucauu auauuaugca aaucgccaua aauccaaaag acaacaacac auuugccagu 480
gcaucccuag auagaacauu gaaaguaugg caauugggag cguccacagc gaauuucaca 540
cuagaagguc augagaaagg cguuaacugu guggacuauu aucacggugg agauaaaccu 600
uauuuaaucu caggcgcuga ugauagauua guaaaaaucu gggauuauca aaacaaaacu 660
uguguucaaa cuuuggaagg acaugcucaa aauguaaccg cugcauguuu ccauccagaa 720
cuuccuguag cucuuacugg aagugaagau gguacuguca gaguguggca ugccaacacc 780
cauagguuag aaaguagcuu aaauuauggc uuugaaagag uauggacuau uuucugccua 840
aagggaucca auaacguggc auuggguuau gaugaaggua gcauuuuggu uaaaguuggu 900
agagaagaac cagcuguuag uauggaugcc aguggaggca aaauuauuug ggccagacac 960
ucugaacuuc aacaggcaaa ucucaaggcg uuagcugaag gugcggaaau aagagaugga 1020
gaacgccuuc caguuucugu aaaagauaug ggugcuugcg agauauaccc ucagacaauu 1080
caacacaauc ccaauggccg uuuuguuguu gucugugggg auggagaaua cauaaucuac 1140
acagcaaugg cuuuaagaaa caaagcguuu gguagcgcac aagaauuugu gugggcucaa 1200
gauuccagcg aauaugccau cagagaaucc ggaucuacua ucagaauuuu uaagaauuuc 1260
aaagagaaga agaauuuuaa guccgauuuu ggagcugaag guauauacgg uggauaccuu 1320
uugggaguca aaucgguuuc ugguuugacu uucuaugauu gggaaacucu cgauuuaguc 1380
agaagaaucg agauacaacc aaaagcaguu uacuggucag auagugguaa auuaguaugu 1440
uuggccacag aagauagcua cuuuauucuu ucuuaugauu cugaugaagu ucaaaaagcc 1500
agagauaaca aucagguugc ggaugaugga guagaaucgg cuuucaaucu ucuaggugaa 1560
auaaacgaau cagugcgaac uggucucugg guaggcgacu guuuuaucua cacgaauucu 1620
guuaaucgua ucaacuacuu cguuggaggu gaacugguua caauugcuca uuuggaccgg 1680
ccuuuguaug ucuugggaua ugugccuaaa gacgauagau uauaccucgu agauaaagag 1740
uugcgcguag uaagcuacca auuacuucuu ucuguucuug aauaucaaac ugccgucaug 1800
agaagagacu uuccaacagc agacagagua cuuccgucca uuccuaagga gcacagaacg 1860
agaguggcac auuucuuaga aaagcaaggc uucaaacagc aagcuuuggc cguaaguaca 1920
gauccagagc acagauucga gcuggcagua gcauuagagg aucuuaauau agccaaaacu 1980
cuagcucaag aagcgaacag uccgcaaaag uggaaucaac uagcagaauu ggcagcugcu 2040
acuaauaaug uaagcguagc caaggaaugu augcaaaaag cgcaagauua uggaggcuug 2100
uugcuucuug cuacgagcuc cggugaugaa aauuuagucc guacucuagg agaaacgaca 2160
caagcugaaa gcaaacauaa cuuagcauuu uugucacacu uguuaguagg ugauuuaaac 2220
aaaugucuag acauucuuau uaauaccggu agauugccag aagcugcauu uuucgccaga 2280
ucuuaccuuc cugauaagau uacagaaguc guggaacugu ggaagacuca guuaucuuca 2340
gucaaucaaa aagcuggaca gagccuugcc gauccuaaaa acuacgaaaa ucuguucccu 2400
gguuuacaag aggcgguggu agcucagaaa uuuuuggaac agcagaauaa agguuuagcg 2460
cccgcaagag uugccaccac cauuccuccu aaucacgaca ggaauguugu agccgaaguu 2520
caagcacaau cgaaacacga uguaccauca uuuaguucuu cguuuauuuc aucagaaaua 2580
gaagcacaaa caaggaguuc ugcuaaaccu gaagaaucuu caaacauuau acagcuggac 2640
caagaugacg acgauaucga uuuagauuug gacgguguaa auaucgauga gaacauugac 2700
acgacggaua ucaacaucga ugaugauuug cugagugauu ga 2742
<210> 154
<211> 1146
<212> RNA
<213> 玉米根萤叶甲属
<400> 154
augcagaucu uuguaaaaac acucacuggu aaaaccauca cccucgaggu ugaaccauca 60
gauaccaucg agaaugucaa agcuaaaauu caagacaaag aagguauucc accagaucaa 120
cagagauuaa ucuuugcugg aaagcaguua gaagauggcc guacucucuc agacuacaac 180
auucagaaag aaucuacacu acacuuagug cuucgucuua gaggagguau gcacaucuuu 240
guaaaaacuc ucacugguaa gaccaucacc cuugagguug aaccaucaga uaccaucgag 300
aaugucaaag cuaaaauuca agacaaagaa gguauuccac cagaucaaca gagauuaauc 360
uuugcuggaa agcaguugga agauggccgu acucucucag acuacaacau ucaaaaagag 420
ucuacccucc auuugguacu ucgucuuaga ggagguaugc agauuuuugu uaaaacuuua 480
acuggaaaga ccaucacccu ugaaguagaa ccuucugaua ccaucgaaaa ugucaaagcc 540
aaaauucaag acaaagaagg uauuccacca gaucaacaaa gauuaaucuu ugccggaaag 600
caauuggaag auggucguac acucucagac uacaacauuc aaaaggaauc uacccuccau 660
uugguacuuc gucuuagagg agguaugcaa aucuuuguaa aaacacucac ugguaagacc 720
aucacccucg agguugaacc aucagauacc aucgagaaug ucaaagcuaa aauucaagac 780
aaagaaggua uuccaccaga ucaacagaga uuaaucuucg cuggaaagca guuggaagau 840
ggccguacuc ucucagacua caauauucag aaagagucua cccuccauuu gguacuucgu 900
cuuagaggag guaugcaaau cuuuguaaaa acucucacug guaagaccau cacccucgag 960
guugaaccau cagauaccau cgagaauguc aaagcuaaaa uucaagacaa agaagguauu 1020
ccaccagauc aacaaagauu aaucuuugcc ggaaagcagu uggaagaugg ccguacucuc 1080
ucagacuaca acauucaaaa agagucuacc cuucacuugg uacuucguuu aagaggagga 1140
aauuaa 1146
<210> 155
<211> 1131
<212> RNA
<213> 玉米根萤叶甲属
<400> 155
augugugacg acgauguagc ggcucuuguc gucgacaaug gcuccggaau gugcaaagcc 60
gguuucgccg gugaugacgc cccucgugcu gucuuuccau ccaucguagg ucgucccaga 120
caccaaggug ucaugguggg uaugggucaa aaagacuccu acguaggaga cgaagcccaa 180
agcaaaagag guauccucac cuuaaaauac cccauugaac acggaauuau cacuaacugg 240
gacgauaugg aaaagaucug gcaucacacc uucuacaaug aacuuagagu agcccccgaa 300
gaacauccca uucuuuugac ugaagcucca cuuaacccaa aagccaacag agaaaagaug 360
acucaaauca uguuugaaac uuucaauacc ccugccaugu auguugccau ucaagcugua 420
uugucucugu acgcuuccgg ucguaccacu gguauuguac uugauucugg agauggugua 480
ucccacacag uacccaucua ugaagguuac gcucucccac acgccaucuu gcguuuggac 540
uuggccggua gagacuugac ugacuaccuu augaagaucu uaaccgaaag agguuacucu 600
uucaccacca cagcugaaag agaaauaguu cgugacauca aggaaaaauu gugcuaugua 660
gcuuuggacu ucgaacagga aauggccaca gcagccagcu ccaccuccuu agaaaagagu 720
uaugaacuuc cugacgguca agucaucacc auugguaaug aaagguuccg uugcccugaa 780
gcucucuucc aaccuuccuu cuuggguaug gaaucuugcg guauccacga aacugucuac 840
aacuccauca ugaagugcga ugucgacauc cguaaagacu uguacgccaa cacuguccuu 900
ucuggaggua ccacaaugua cccugguauu gccgaucgua ugcaaaagga aaucacugcc 960
uuggcuccau caaccaucaa aaucaagauc aucgcucccc cagaaagaaa guacuccguu 1020
uggaucggug gcuccaucuu ggccucccuc uccaccuucc aacagaugug gaucuccaaa 1080
caagaauacg acgaauccgg cccuggaauu guucaccgca aaugcuucua a 1131
<210> 156
<211> 2640
<212> RNA
<213> 玉米根萤叶甲属
<400> 156
auggguacuu uuaaaagaga uacucaugau gaggacgggg gaucaagugc uuuucaaaau 60
cuggagaaaa cuacuguuuu gcaggaagcu agaguuuuua augaaacuag uguaaaucca 120
agaaaaugua caccgauacu aacaaaacug uuguacuuau ugaaccaggg ugaaacuuua 180
agugccaaag aggccacaga uguuuucuuu gccaugacca aacuguucca aucaaaagau 240
guaauauuga gaaggauggu uuauuuggga auuaaagaac ucaguucugu ugcugaugau 300
gucauuauug uaacauccag ucuuacaaaa gauaugacug guaaagaaga cauguacaga 360
gcagcugcua uaagagcauu augcaguauu acugaugcua cuaugcuuca agcuauagaa 420
cguuauauga agcaagcuau uguagauaga aacgcagcug ucaguucagc agcacuaauu 480
aguucauuac auaugagcaa auuagcucca gauguaguaa aaagaugggu aaaugaagcu 540
caggaagcag uaaauaguga uaaugcaaug guacaguauc acgcauuagg ucuucuauac 600
cauauuagga agacugauaa gcuagcagug acaaaauuga uuuccaagcu gaauucaaug 660
gguuuaaaga gcccuuaugc uuuguguaug uugauaagaa ucacugcaaa acuuuuagaa 720
gaagaggacc aagagucacu ccucaacucc ccauauacaa uaauauuuac aaugggcuua 780
aggaacaaau cugaaauggu gguguaugaa gcugcacaug ccaugguuaa ccugaaguuc 840
acgaguagua augugcuagc acccgcuaua aguguucuac aacuauuuug uggaucuccu 900
aaagccacac ucagauuugc ugcuguuaga acuuuaaauc aaguggccac cacccacccu 960
gcgucaguga cagcuuguaa uuuggaucua gaaaauuuga uuacugaucc uaauagguca 1020
auugcuacac uggccauuac uacucuuuug aaaacaggug ccgaaucuuc uguugacaga 1080
cuaaugaaac aaaucgcuac uuuuguaucu gaaaucagug augaauuuaa agugguuguc 1140
auucaggcaa uuaagguauu agcuuugaaa uuuccaagga aacauagcac gcuuaugaau 1200
uuccuauccg ccauguuaag agaugaggga gguuuagaau auaaagcauc cauagcagau 1260
accauuauaa cccuaaucga agauaauccc gaagcuaaag aaucugguuu ggcgcaucuu 1320
ugcgaguuca uugaagacug ugaacauguu ucuuuggcug ugagaaucuu gcauuuguua 1380
ggaaaggaag gacccaagac caaacaacca ucgagauaca uccguuuuau cuacaaucgc 1440
gucauauugg aauguccuuc uguaagagcu gcugcagucu ccgccauggc acaauucgga 1500
gccucuuguc ccgauuuguu agaaaauauc caaauauuac uuucgaggug ucagauggau 1560
ucagacgaug aaguuaggga cagagcuaca uauuauagua auauacuuaa caaaaaugau 1620
aaaaguuuau acaacaauua cauuuuggau ucuuugcagg uuucaauucc uucacuagaa 1680
agaucgcuua gagaauacau ucaaaaucca acugacgaac cauuugacau uaaguccgua 1740
ccuguagcag cagugccaac agcagaagaa cgagaaguua aaaacaaauc ugaaggacug 1800
cuagucucuc aagguccagu ccgaccuccu ccggugucua gagaagaaaa cuucgccgaa 1860
aaacuuagua acguuccggg uauacaacag uuaggaccuu uguucaaaac uuccgacguc 1920
guugaacuca cugaaucuga aacagaguau uuuguccgcu guaucaagca cuguuucaaa 1980
caucacaucg uccuccaauu cgauugucug aauaccuugc cagaccagcu uuuagaaaac 2040
guuagagugg agauagacgc cggugaaacc uucgaaauuu uggcagaaau accuugugaa 2100
aaguugcacu auaacgaaac cgguaccaca uauguaguag uuaaguugcc ugaugaugau 2160
cuccccaacu cuguugguac guguggagcc guguugaagu ucuuagugaa agauugugau 2220
ccaucaacgg gaauaccaga uucugaugag gguuacgaug augaauauac acuggaagac 2280
aucgaaauaa cauuagggga ccaaauucaa aaaguaagca aaguaaauug ggcugcagcc 2340
ugggaagaag cugcagcuac uuauguagaa aaagaggaua cauacuccuu gaccaucaau 2400
acgcuaagug gcgcuguuaa gaauauuauu caguucuugg gauuacagcc ugcggaaagg 2460
acugacagag uaccggaggg uaaaucuacg cacacauuac uucuugcugg uguauucagg 2520
ggagguauug acauacuagu aagagcgaaa cuagcuuugg gcgaaugugu uacgaugcaa 2580
cuaacaguca ggucgccaga uccugacguu gcugagcuua uaacuucaac uguagguuaa 2640
<210> 157
<211> 651
<212> RNA
<213> 玉米根萤叶甲属
<400> 157
auggcggcaa acagaacugg accugcucag agaccaaaug gcgcuaccca aggaaagaua 60
ugucaguuca aacugguccu acuaggcgaa agugccgucg guaagucgag uuugguacug 120
agguucguca aaggacaguu ccacgaauac caggagagua ccauaggagc agcuuuccuu 180
acacaaacca uaugccucga cgauacaacu guuaaauuug aaauuuggga cacagcgggu 240
caagaaaggu accacaguuu agcuccuaug uacuauaggg gcgcacaggc agcuauaguc 300
gucuacgaca uaaccaauca agacacauuc ggcagggcga aaacgugggu gaaggaacuu 360
caaaggcagg ccaguccgac gaucgugaua gcuuuggccg gcaacaagca agauuuggcc 420
aacaaacgua ugguagaaua cgaagaggcg cagacguaug cugacgaaaa cggcuuacuu 480
uuuauggaaa cuuccgcaaa gacggcaaug aacgucaacg auauauuuuu agcaauagcu 540
aagaaacugc ccaagaauga acaaaccaca ggucaaggcg gcagugccca aggcaggcgg 600
cuagcggagg gcgauucggg cgccaaggca cccggaaauu guugcaagug a 651
<210> 158
<211> 279
<212> RNA
<213> 玉米根萤叶甲属
<400> 158
augaaguuuu uaagaucgac agugugcuac auugccaucu uggcaauucu cuuuacccuc 60
ugugccgaug agguugaagg aaggagaaaa auuuugaugg ggcgaaaaag cauuaccagg 120
acauaucuuc guggaaaugc uguuccugcg uaugugauaa uaauccuugu aggaauuggu 180
caaaucaucc ugggagggau auuguacguu gcauugagga agaagaucau ugcugcaccu 240
guaacggcau cauaugcagu ggcuagacaa gaaccauaa 279
<210> 159
<211> 474
<212> RNA
<213> 玉米根萤叶甲属
<400> 159
augcagaucu ucguuaaaac cuuaacgggu aagaccauca cucuugaggu cgagcccuca 60
gauacuaucg aaaaugugaa agcuaaaauc caggauaaag aaggaauucc cccagaccag 120
caacgucuca ucuucgcugg aaaacaacuc gaagaugguc guaccuuguc ugacuauaau 180
auucaaaaag aaucaacccu ucacuuggug uugagauuga gaggaggugc uaagaaacgu 240
aagaagaaga auuacuccac ccccaagaaa aucaagcaca agaagaagaa gguuaaguua 300
gcuguauuga aauuuuauaa gguugacgaa aaugguaaaa uccaccgauu gagacgugaa 360
ugccccgcug aacaaugugg agcugguguc uucauggcag ccauggaaga caggcauuac 420
uguggcaagu gcgguuacac ucuugucuuc uccaaaccag gagaugagaa auag 474
<210> 160
<211> 747
<212> RNA
<213> 玉米根萤叶甲属
<400> 160
augaugucca aagcagacac acaggaagau gccuccuucg ccaaauugga aaaucagauu 60
gcuaucauca aauacguaau acucuuuacc aacguuuugc aaugggcucu cggugcagca 120
aucuucgcuc uuugccuuug gcuacgauuc gaggagggca uucaagaaug gcuccagaaa 180
uuggauucag aacaauuuua caucggagua uauguacuua uagucgcuuc acugaucguc 240
augauugugu ccuuuauagg auguauuagu gcccugcagg agaguaccau ggcccuuuua 300
guguacaucg gcacccaagu gcucaguuuu auauucgguu uauccgguuc ggcgguucuu 360
cuggauaaca gcgccagaga uucccacuuc caaccgagga uccgagagag uaugcgacgu 420
cuuaucauga augcucauca cgaccaaucc agacaaacac uagccaugau ucaggaaaau 480
guugguugcu gcggagcuga uggcgcaaca gacuaccucu cucuucagca gccccuucca 540
agucagugca gagacaccgu uacuggaaac ccauucuucc acggaugugu agaugaacuc 600
accugguucu ucgaagaaaa augugguugg auagcagguu uagcuauggc gauaugcaug 660
auuaacgucc uuaguauugu uuuaucuacg guacucaucc aggcauugaa aaaagaagaa 720
gaagcauccg auucauacag gagauag 747
<210> 161
<211> 375
<212> RNA
<213> 玉米根萤叶甲属
<400> 161
augucuggac guggcaaggg aggcaaagua aagggaaaag caaagucccg aucaaaucgu 60
gcugguuuac aauuuccugu aggucguauu caucguuuau ugagaaaagg aaauuaugcc 120
gaaagaguug gugcuggagc uccuguauac uuggcagcug uuauggaaua uuuagcugcu 180
gaaguuuugg aauuggcagg aaaugcagcu agagauaaca aaaagacccg uauaauuccu 240
agacauuuac aauuggccau aagaaaugac gaggaauuga acaaauuacu gucaggaguu 300
accaucgccc aagguggagu auugccuaau auacaagcag uacuguuacc uaaaaaaacu 360
gaaaagaaag cuuaa 375
<210> 162
<211> 864
<212> RNA
<213> 玉米根萤叶甲属
<400> 162
augaaguugg augguguaga ucugccccca ccaauuagcu ucgacauugc ggaagagcaa 60
ccguuaccac cuugccaaca gacguucuua uguaauggug auggaggauc cauagugcga 120
caguuucucg agcuguauuu cguaauauau gauucagaua auaggcaguc ccuucuucag 180
gcauaucacg aaaaagccac auuuucaaug acaauggccu acccguacgg cuauuccaaa 240
gacaguaaag gaguaucgug guugaauugg uaugccaccg auaauagaaa uuuauuacga 300
guucaagauc cagacagaag aaacaaguug uuaagacagg gacaaguugc uguaguuucg 360
uucuugcaag auaugccgca cacgaagcac gauauucaca guuuuacagu agauuugaca 420
guuuuuacac cccagauguu auguuugaca guggcuggua uguuuaaaga auugaaaagu 480
ggccacaaag uaccuccuuu aagauauuuc uucagaaccc uuguaauugu accugcugga 540
ucagguuuuu gcauagcaaa ugaagaacuu cacauaucca augcaacucc ggaccaagca 600
aaagaugcuu ucaagaccac cguuaaugua gcuccggcac cagccccugu gauuaccucu 660
ccuggaccca guauaccaca acccgcugug ccagaugaug cuacaaaaca agaaauggua 720
aaacagaugu ccgcaguauc cggaaugaau cucgaguggu cgcuacagug ucucgaagaa 780
acacaauggg acuaccagaa agccauaaug guauuccaaa auuuaaacgc acaagguguu 840
guaccacaag cagcauuuau uaaa 864
<210> 163
<211> 2868
<212> RNA
<213> 玉米根萤叶甲属
<400> 163
augacugcgg uagaacaacc uuguuacaca cuaauaaacu ugccaacaga uucggagccc 60
uacaaugaaa ugcaacuaaa aauggauuua gaaaagggug agguuaaagu aaaaauaaga 120
gcauuagaaa aaauaauuca caugauucug gcaggagaaa gguugccgaa uggauuucua 180
augaccauca uaagaaacgu uuuaccuuua caagaucauu uggcaaaaaa acuauuauug 240
auuuucuggg aaauaguucc aaaaacaaau ccagaaggua aacuacuaca agagaugauu 300
uugguaugug augccuauag aaaagaucug caacacccaa augaauuuuu gagagguucu 360
acacuucgcu ucuugugcaa acugaaggaa ccagaauugu uggaaccauu aaugcccagu 420
auuagagcuu guuuggauca uaggcacagc uaugugagga ggaaugcugu acuggcaauu 480
uuuaccauuu acaaaaauuu ugaagcccuc auuccagaug cuccugaacu gaucuccaau 540
uauuuggaug gugagcaaga caugucuugu aaaagaaaug cguuuuuaau gcuucuucau 600
gcugaccaag aaagggcguu gucguauuug gcaucauguu uagaucaagu aaauucauuu 660
ggagauauuc uacaacuggu caucguugag uugauauaua agguguguca uuccaauccu 720
gcggaaagau cuagauuuau uagauguaua uauaacuugu ugaacucaag caguccugcu 780
gucagguacg aagcugcagg aacuuuaguc acccucucca gugccccgac ugccguuaaa 840
gcugcugcua gcuguuacau ugaguuaauu aucaaagaaa gugacaacaa uguaaaacuc 900
aucguuuugg acaggcugau agcacuuaag gagcuuccua aucacgaaag aauucugcag 960
gauuuaguua uggacauacu gagaguacuc ucugcuccug acuuagaagu ccgcaagaag 1020
acuuuaaguc uagcccuuga auuagucucu ucacggaaca uagaagaaau gguauuagua 1080
uuaacaaagg aagugaguaa aacgguagac agugaacaug aggauacagg aaaguacagg 1140
caauuguuag uaaggacucu acauucgugu uccauuaagu ucccagauau cgcacguagu 1200
guuauaccag ucuugauuga auuuuuaucc gauaauaaug aacuggcugc cacagaugua 1260
uugcuguucu uaagggaagc cauacagaag uuuaaagaau ugcaaccguu aauuauugag 1320
aaacucaucg aaacuuucaa agacauuaaa uuggucaaag uccauagagc agcaauuugg 1380
auuuugggag aauacgcgag uacugcuucc gauauagaag uuaucguugg agaaauuaac 1440
agauuguugg gugaaggauc ccucguugaa gcugagcaga aguuaauagc aggagauacg 1500
gaagagaaug cuccugcacc ugcugcaggc gccaccacuu uaguuacuuc cgauggaaca 1560
uaugcuaccc aaucagcuuu caacacuguc agccaaacca cuaaagaagc acgaccuccu 1620
cuaagacaau accucaugga uggugauuuu uucaucggag ccucuuuggc aucuacauua 1680
accaaacugu cuuugcggua ugaggaccuc accucuccug cugcuagcaa uggauucaau 1740
gccaaaauua ugcuuauuau ggcuggaauu cuucacuugg gaaaaucagg acuucccaca 1800
aaaucaauaa ccaacgacga uaaagaccac auucuguucu guuuacgagu ccuaucugau 1860
cguucuccaa ucauuguuga aauuuucaaa aaauugugcc gcucggcacu aaaugagaug 1920
cuucuagcua aggaaucggu agaagcgauc ucgcaaaaga gcaaagaaaa aaacaagcgu 1980
acgauucaaa cugacgacgc uauaagcuuc cugcaauuag agacagauaa aaguggagag 2040
cuaggagaaa acguauucga gaugucgcug ucacaagcuu uaguaggagg ucgaacggga 2100
gguggcgaau caguauuaag uuccaauaaa uuagauaaaa ucacacaacu gacugguuuu 2160
uccgauccag uuuauuccga agcauacguu cacgugaauc aguacgauau cgugcuugau 2220
gucuuaaucg uaaaccaaac uaacgauacu uuacaaaacu gcacgcuaga gcuggcuacu 2280
uuaggcgauu ugaaguuggu agagaagcca caaccugucg uauuggcgcc caaagacuuu 2340
ugcaacauua aagcuaacgu gaaaguggcc ucaacugaaa acggaauuau auuuggcaac 2400
auuguguaug augucauagg agcgggguca gauaggaaug uuguaguuuu gaaugauaua 2460
cacauagaua uaauggacua uauagugccu gcuaguugua cagauagcga guuuaugaga 2520
augugggcgg aauuugaaug ggaaaauaag guaaccguua acacaccccu cacggaacuu 2580
ucagaauacc ucgaacaucu acucaaaagc acaaauuuga aauguuuaac aucagaaaaa 2640
gcucugagcg ggcagugugg uuuuauggca gccaauuuau augcaaaauc cauuuuugga 2700
gaagacgcuu uggccaacuu aaguauagag aaaccuuuua auaaacccga ugcgccagua 2760
agcggucaua uuagaauaag ggccaaaagu cagggcaugg ccuuaaguuu aggagacaaa 2820
gucaauauga cacagaagag cacacaacau aaaguaguag cugcauaa 2868
<210> 164
<211> 549
<212> RNA
<213> 玉米根萤叶甲属
<400> 164
auggguaaug uguuugcaaa uuuauucaaa ggccucuuug gcaaaaagga aaugaggaua 60
uugaugguag gacucgaugc agcugguaaa accacaauuu uauauaaacu uaaauuagga 120
gaaauuguaa caacuauucc aacaauugga uuuaaugugg agacuguaga auauaagaac 180
auuaguuuua caguauggga uguagguggu caagauaaaa uuaggccauu guggagacac 240
uauuuccaaa acacacaagg ccuaauuuuc guaguagaca guaacgacag ggaacguauc 300
acugaggcua aagaugaauu aaugcguaug uuggccgaag augaacuuag agaugccgua 360
cuucucauuu ucgccaacaa acaagauuug cccaaugcaa ugaacgcugc agaaaucacc 420
gacaaacucg gucuccauuc acuacgcaac cgcaacuggu acauucaagc uaccugugca 480
acuagcggag auggucucua ugaaggucug gacugguugu ccaaucaauu aaagaacgcc 540
aaucgcuag 549
<210> 165
<211> 4425
<212> RNA
<213> 玉米根萤叶甲属
<400> 165
augggacggu ugcacuguuu auuuuguauu uuccuauguu uuaccgucau caacacgcag 60
acaacgaaua uacauggauu cucggaaaau uccguggaua cauuucuauc accucauggg 120
aaaagugcaa aauucgugca ccaaaaucac aaacccaaaa uugaaaauug ucagaacuac 180
aaacccucgg ugaaagaaga acagccaggc ggaacguacg uaacaacggu uaccgcuauc 240
gaugacgauc cuagggaggg aggaggaaca auuaguuaca aacuaauuca uagagaagga 300
gaacauguuu uauuugacau agacaacguu acugguguuu ugacaacuau ccagccauuu 360
gaucgggaug aaccaguaag gcagaaggaa cuuuauguaa ccguacaagc uucagacaac 420
ggcaggccac cauuagcaga ugucuguaca uuuacaguua ccauuaccga cauuaaugau 480
aaugcgccac agcuugauaa acugaaauac gaugcacaag uuucugaaga uuuaaaagua 540
ggaagugaag ugaugagagu uuuugcuuac gacauugaug auggggaaaa uucaagauua 600
ucguauaacu uuucaaacga aaaugcucaa uucacccagu auuucaggau agaucgagau 660
acuggcguug uguauuuaaa ggaagcuuua acagacaaaa agaauacuag auuuaacagu 720
gcuguuuaug uagccgauaa uggcguuaac gaucaagaag gccaaaaaga uucaaccgcu 780
aagauaucua uaacaguagu agggucugau aaacagccuc ccagauuuac ucaaaaaaug 840
ccugauggaa ucuuggagau ccccgaagau uuuaaagacu uuucuaaaca uauugucaca 900
gucgaagcaa cguccaacau ugcggaucca caacuugcuu uugaauuggu gaagggaaag 960
acauaucaaa ccaauaaaga ccaaacguuu cuuuuggagg cagaaggaaa uaaagcgcac 1020
auaaagcuag ugcguccacu ggauuaugaa acaguaacgg aauauacucu aacuauucga 1080
guaaaaaaca aagauuuaau ggauucuucc auaaauauac caauuaaagu auuagauguu 1140
aaugaugaaa uuccuaauuu ccuugaauuu cuuaaaggua gugucgugga aaaugacaag 1200
ccaggugcac aagcgauuca aguaagagca aucgauaaag acggaacugc ugcuaacaac 1260
auugugagcu augaacucgu ugacaauaca gauuuguuug caauaaaccg aucuacggga 1320
guaauuacgu cgagagugga guuugaucgu gaaacuguac cucuauauca cguaaacguu 1380
aaagcuuaug auaacucucc gucugcuuug uauaacacga cauugccuaa cauuguaauu 1440
cagacauucc aaaucaguau agaagaucaa aaugacaaca aaccuguauu uacucaucca 1500
auuuaucagu ucaguaauau uacugagcuu gcugauaaau cgaguauugu uggugaaguc 1560
aaagcuuuag auaaugacac ggcuucaguu auaaguuaua guauuacaaa uggaaauauu 1620
gacgaugcgu uuaugauuga aaauucuacc ggcagaauaa gaguuaaugg aaaacuggau 1680
uacgagaaaa ucgaacaaua caacuuaacc guucgcgcau uugauggggc auuugaagau 1740
uuugcaauug uuuuaauuuc cauacuuaau gaaaaugacg aaccuccagu uuuugacgac 1800
uauaucagag aaauucaaau uaaagaggaa gaaccuauga uauccggaug cguuguuaga 1860
gugacugcuc augauccaga uauuaaagac aggcaugcug aucaacacau aguauaugag 1920
gucgcgaaag aacagaaaga uuuuuugacc guaucugccg auggaugcgu acaaguaaca 1980
aaaccucucg accgagaucc gccuuucggu agcccaacac gacaagucuu caucuaugcu 2040
cgugauaaug auggaggcac aaauucauug uuggccacug cagaaauuga aauuauuuua 2100
auagauauaa acgauaaugc ucccuuuuua aauguuacag aaauuguuua uuaugaaaac 2160
caggauccag guuuuauagg uaaccuaagu gccgaugauu acgauggucc ugauaaugga 2220
ccuccguuug cuuuucgauu aucagacacu gcuucagaua guauuagauc gaaauuuucc 2280
auuaucggaa accagcuuuu cgcuuuagaa auguuugaua gagaagagca aaaauauuau 2340
gacauugcca uugacauuac agauagugga guaccuccac uaacaggaac uaguauucuu 2400
agaguuauaa ucggagaugu aaaugauaau ccagcuacag acggaaacag cacgaucuuu 2460
guguauaagu acgucaaugg gccagaaaau uucauggaaa ucggacgugu auauguuaca 2520
gaccuagacg auugggauuu aaaugacaaa gucuuuguuc aagaagauaa cuuugaugaa 2580
uuuguguuaa accagcauaa caacgguaug auucugauga aaccaacaac ggcugaggga 2640
acuuaugagg uucauuacag ggucacugaa acccaugaac ccacaauaca cgaacauaca 2700
guuaaugcaa uagucacgau uacaguuaaa guacuuccag aggaagcggu uguaaaauca 2760
ggaucaauuc gauugagagg aacaacuaag gaagaauuca uagaaaauuc auugaaugga 2820
aagagcaaaa gagacauauu acaccaagaa cucuccaaaa uauuaaauac aucuuuagcg 2880
aauguugaug uauuuacugu uuuaaauuca ccccaccaga auaguucguu uguggauguu 2940
cgauuuucug cucauggauc uccauauuau gcuccagaga aacucgaaaa caaaguuaca 3000
gaucaucaaa uggagcuuga acaaaaauua gauguggaau ucuacaugau caacguaaac 3060
gagugccuua acgaaacaac guguggagcu gaaaacucau guacgaacaa auuaaacaua 3120
acacgagaac cagcuguagu guuuacuaac agaacauccu uugucggugu aaaugcauuu 3180
auugauccug ugugugccgc uuuaccaaga gauguuaugg aauguuucaa cggaggcguc 3240
cuuaucgaaa acacagcgug uaauuguccu gcaggauuug aaggaccaca uugugaaauc 3300
cuagcuauag gauuuacagg aacugguugg gcuauguauc cauccuuuga cgcuacaaac 3360
aggacugaga uuauacugca uauuuuauca caaacugaua augguuugau auuuuacaau 3420
ggaccuuuaa auauaagaca aacuucuuug ucuaaagauu auauaucauu agaacuuaaa 3480
gacggauauc cauuacuuca aauuugcacc ggcucaagca cucaagaaau uuaucugaaa 3540
gagcgcauuc acaaauugag cgauggaucg uuacacaaaa uaaaaauagg aucuggauuu 3600
gacgauauau cccuggaagu agacgacugu ggaacaacgu guucaauuug gacuaauaaa 3660
cuacauaaag guguuauccg agcaaauggc ccccuucaac ugggagguau gaaaaacaga 3720
uucaccgauc aagaauucaa acgaauuugg gaccauuugc caccgacugc cacccguuuc 3780
ucugguugua uuagaaauuu gacguauaau gaauuuuacu acaaccucgg ugcaccuucu 3840
gaugcauucc aagcguaucc cgacuguaac uaugcaguga ugcaagcugu gacuuucggu 3900
aucgacucca auuucuuggu ugcuauucug guuuguguag caauuuugau aauucuucuu 3960
cuggcaguag uuguacauag acguaaacac gacaacuuua acgaaaaaga aaucgaugau 4020
acucgcgaaa acauuaucaa cuacgaagau gaagguggcg gcgaauguga caccaacuac 4080
gaccugucug uuuuccauca gaacaacauu guggacgaaa aaccauugau gagagacaac 4140
cccgauguac cugcagauau aaguggcuuu uuagauaaca agaaagacaa cugugauaaa 4200
gaccccgaua auuugccuua ugacgacguu cgccauuaug ccuacgaggg agacggaaau 4260
agcaccggau ccuuaucuuc ucucgcuuca uguacggacg aaggagauuu aaaguucaac 4320
uacuuaucaa guuuuggacc cagauucaga aaguuagccg acauguaugg agaagaucca 4380
agcgaugaag acucacacga uggaaacgaa gaauccuggu gcuag 4425
<210> 166
<211> 291
<212> RNA
<213> 玉米根萤叶甲属
<400> 166
augccuuucu guggucccaa auugucccuc ugcggccuga uuaucagugc augggguauc 60
auccaguugg guuucauggg uguauucuau uacauugggg cuguggcuuu agcagaagau 120
auuccagagg uugaguuuaa gggcgauuua gacaaauuuu auagcgacgu caacacgggu 180
uucacacaga augcuuacaa cugcuggauu gcugcucucc uauaccugau aacauuagca 240
guaucagcuc accaauucug ggccaacaac agaucaucau ugaacgucua a 291
<210> 167
<211> 540
<212> RNA
<213> 玉米根萤叶甲属
<400> 167
augggucuua ccauaucagc aguguuuaau agguuguuua guaaaaagcc uaugagaauu 60
uuaaugguag gauuagaugc cgcagguaaa accacaaucu uauacaaauu gaagcuuggu 120
gaaaucguaa cuacaauacc aaccaucggc uucaauguag aaaccguuga guacaagaau 180
auaucuuuca cgguauggga uguagguggc cagacgagaa ucagaaaacu cuggagacac 240
uauuucgcca acacugaugg acucauuuuu gugguugauu ccaacgaccg agaccguauc 300
gcggaagccg aagaagaauu gcacaauaug uuaggagagg acgauuuaag agacugcauu 360
uuguuaauau ucgccaacaa acaagauuua ccgaacucga uguccacugc ugaauugacc 420
gauaagcuua aguugcacac uuugaagaau aggagguggu acauacaagc cacaugugcu 480
acucaaggga augguuugua cgaaggacua gauugguugu cgaaugaauu ggccaaguga 540
<210> 168
<211> 618
<212> RNA
<213> 玉米根萤叶甲属
<400> 168
augcgguaca cuuugaguua caucggugcu acccuagcgu ccacuguaac acugauuuuu 60
gcccucuacu acugccucac gggaaaagga gagcaaguua guuuagcaug guuauuguug 120
aaugugucuc cccacaugug ggcaggucua ggaauuggcc uugcuguauc auuaucaguu 180
guaggagcug cugcaggaau ucacacuaca ggagucagua ucguaggagc ugguguuaaa 240
gcccccagaa ucaaaaccaa aaauuuaauu ucuauuauuu ucugugaagc uguggcuauc 300
uauggguuaa uuauggcuau aguacucugu ggaaguugga agaauuucga uguagaccua 360
uucaaccuca aaacucauaa cuuugcucaa aaccauuaug gaucacaugu uauuuuugga 420
uccgguuuaa cuguuggauu uguaaaucua uuauguggau uuuguguugg aguaguuggu 480
ucuggugcag ccauuucuga ugcagccaau ucaucauuau ucgucaaaau uuugauuauu 540
gagauuuuug gaagugccau uggucucuuc ggucugauug uuggaguaua cuugacguca 600
agaggcucua ugguuuaa 618
<210> 169
<211> 5694
<212> RNA
<213> 玉米根萤叶甲属
<400> 169
auggcuacca acgauaguaa agcuccguug aggacaguua aaagagugca auuuggaaua 60
cuuaguccag augaaauuag acgaauguca gucacagaag ggggcauccg cuucccagaa 120
accauggaag caggccgccc caaacuaugc ggucuuaugg accccagaca aggugucaua 180
gacagaagcu caagaugcca gacaugugcc ggaaauauga cagaaugucc uggacauuuc 240
ggacauaucg agcuggcaaa accaguuuuc cacguaggau ucguaacaaa aacaauaaag 300
aucuugagau gcguuugcuu cuuuugcagu aaauuauuag ucaguccaaa uaauccgaaa 360
auuaaagaag uuguaaugaa aucaaaggga cagccacgua aaagauuagc uuucguuuau 420
gaucugugua aagguaaaaa uauuugugaa gguggagaug aaauggaugu ggguaaagaa 480
agcgaagauc ccaauaaaaa agcaggccau ggugguugug gucgauauca accaaauauc 540
agacgugccg guuuagauuu aacagcagaa uggaaacacg ucaaugaaga cacacaagaa 600
aagaaaaucg cacuaucugc cgaacguguc ugggaaaucc uaaaacauau cacagaugaa 660
gaauguuuca uucuugguau ggaucccaaa uuugcuagac cagauuggau gauaguaacg 720
guacuuccug uuccuccccu agcaguacga ccugcuguag uuaugcacgg aucugcaagg 780
aaucaggaug auaucacuca caaauuggcc gacauuauca aggcgaauaa cgaauuacag 840
aagaacgagu cugcaggugc agccgcucau auaaucacag aaaauauuaa gauguugcaa 900
uuucacgucg ccacuuuagu ugacaacgau augccgggaa ugccgagagc aaugcaaaaa 960
ucuggaaaac cccuaaaagc uaucaaagcu cggcugaaag guaaagaagg aaggauucga 1020
gguaaccuua ugggaaagcg uguggacuuu ucugcacgua cugucaucac accagauccc 1080
aauuuacgua ucgaccaagu aggagugccu agaaguauug cucaaaacau gacguuucca 1140
gaaaucguca caccuuucaa uuuugacaaa auguuggaau ugguacagag agguaauucu 1200
caguauccag gagcuaagua uaucaucaga gacaauggag agaggauuga uuuacguuuc 1260
cacccaaaac cgucagauuu acauuugcag ugugguuaua agguagaaag acacaucaga 1320
gacggcgauc uaguaaucuu caaccgucaa ccaacccucc acaagaugag uaugaugggc 1380
cacagaguca aagucuuacc cuggucgacg uuccguauga aucucucgug caccucuccc 1440
uacaacgccg auuuugacgg cgacgaaaug aaccuccaug ugccccaaag uauggaaacu 1500
cgagcugaag ucgaaaaccu ccacaucacu cccaggcaaa ucauuacucc gcaagcuaac 1560
caacccguca uggguauugu acaagauacg uugacagcug uuaggaagau gacaaaaagg 1620
gauguauuca ucgagaagga acaaaugaug aauauauuga uguucuugcc aauuugggau 1680
gguaaaaugc cccguccagc cauccucaaa cccaaaccgu uguggacagg aaaacagaua 1740
uuuucccuga ucauuccugg caauguaaau augauacgua cccauucuac gcauccagac 1800
gacgaggacg acggucccua uaaauggaua ucgccaggag auacgaaagu uaugguagaa 1860
cauggagaau uggucauggg uauauugugu aagaaaaguc uuggaacauc agcagguucc 1920
cugcugcaua uuuguauguu ggaauuagga cacgaagugu gugguagauu uuaugguaac 1980
auucaaacug uaaucaacaa cugguuguug uuagaagguc acagcaucgg uauuggagac 2040
accauugccg auccucagac uuacacagaa auucagagag ccaucaggaa agccaaagaa 2100
gauguaauag aagucaucca gaaagcucac aacauggaac uggaaccgac ucccgguaau 2160
acguugcguc agacuuucga aaaucaagua aacagaauuc uaaacgacgc ucgugacaaa 2220
acuggugguu ccgcuaagaa aucuuugacu gaauacaaua accuaaaggc uauggucgua 2280
ucgggaucca agggauccaa cauuaauauu ucccagguua uugcuugcgu gggucaacag 2340
aacguagaag guaaacguau uccauuuggc uucagaaaac gcacguugcc gcacuucauc 2400
aaggacgauu acgguccuga auccagaggu uucguagaaa auucguaucu ugccggucuc 2460
acuccuucgg aguucuauuu ccacgcuaug ggaggucgug aaggucuuau cgauacugcu 2520
guaaaaacug ccgaaacugg uuacauccag cgucgucuga ucaaggcuau ggagagugua 2580
augguacacu acgacgguac cguaagaaau ucuguaggac aacuuaucca guugagauac 2640
ggugaggacg gacucugugg agagauggua gaguuucaau auuuagcaac ggucaaauua 2700
aguaacaagg cguuugagag aaaauucaga uuugauccga guaaugaaag guauuugaga 2760
agaguuuuca augaagaagu uaucaagcaa cugauggguu caggggaagu cauuuccgaa 2820
cuugagagag aaugggaaca acuccagaaa gacagagaag ccuuaagaca aaucuucccu 2880
agcggagaau ccaaaguagu acuccccugu aauuuacaac guaugaucug gaauguacaa 2940
aaaauuuucc acauaaacaa acgagccccg acagaccugu ccccguuaag aguuauccaa 3000
ggcguucgag aauuacucag gaaaugcguc aucguagcug gcgaggaucg ucuguccaaa 3060
caagccaacg aaaacgcaac guuacucuuc cagugucuag ucagaucgac ccucugcacc 3120
aaaugcguuu cugaagaauu caggcucagc accgaagccu ucgagugguu gauaggagaa 3180
aucgagacga gguuccaaca agcccaagcc aauccuggag aaaugguggg cgcucuggcc 3240
gcgcagucac ugggagaacc cgcuacucag augacacuga acacuuucca uuuugcuggu 3300
guauccucca agaacguaac ccugggugua ccuagauuaa aggaaauuau uaauauuucc 3360
aagaaaccca aggcuccauc ucuaaccgug uuuuuaacug gugcggcugc uagagaugcg 3420
gaaaaagcga agaauguguu augcagacuu gaacacacca cucuucguaa aguaaccgcc 3480
aacaccgcca ucuauuacga uccugaccca caaaauaccg ucauuccuga ggaucaggag 3540
uucguuaacg ucuacuauga aaugcccgau uucgauccua cccguauauc gccgugguug 3600
cuucguaucg aacuggacag aaagagaaug acagauaaga aacuaacuau ggaacaaauu 3660
gcugaaaaga ucaacgcugg guucggggac gauuugaauu guauuuucaa cgacgacaau 3720
gcugaaaagu uggugcugcg uaucagaauc augaacagcg acgauggaaa auucggagaa 3780
ggugcugaug aggacguaga caaaauggau gacgacaugu uuuugagaug caucgaagcg 3840
aacaugcuga gcgauaugac cuugcaaggu auagaagcga uuuccaaggu auacaugcac 3900
uugccacaga cugacucgaa aaaaaggauc gucaucacug aaacaggcga auuuaaggcc 3960
aucgcagaau ggcuauugga aacugacggu accagcauga ugaaaguacu gucagaaaga 4020
gacgucgauc cggucaggac guuuucuaac gacauuugug aaauauuuuc gguacuuggu 4080
aucgaggcug ugcguaaguc uguagagaag gaaaugaacg cuguccuuuc guucuacggu 4140
cuguauguaa acuaucgcca ucuugccuug cuuugugacg uaaugacagc caaaggucac 4200
uuaauggcca ucacccguca cgguaucaac agacaagaca cuggagcucu gaugaggugu 4260
uccuucgagg aaacuguaga uguauugaug gacgcugcca gucaugcgga ggucgaccca 4320
augagaggag uaucugaaaa cauuauccuc ggucaacuac caagaauggg cacaggcugc 4380
uucgaucuuu ugcuggacgc cgaaaaaugu aaaaugggaa uugccauacc ucaagcgcac 4440
agcagcgauc uaauggcuuc aggaauguuc uuuggauuag ccgcuacacc cagcaguaug 4500
aguccaggug gugcuaugac cccauggaau caagcagcua caccauacgu uggcaguauc 4560
uggucuccac agaauuuaau gggcagugga augacaccag guggugccgc uuucucccca 4620
ucagcugcgu cagaugcauc aggaauguca ccagcuuaug gcgguugguc accaacacca 4680
caaucuccug caaugucgcc auauauggcu ucuccacaug gacaaucgcc uuccuacagu 4740
ccaucaaguc cagcguucca accuacuuca ccauccauga cgccgaccuc uccuggauau 4800
ucucccaguu cuccugguua uucaccuacc agucucaauu acaguccaac gagucccagu 4860
uauucaccca cuucucagag uuacucccca accucaccua guuacucacc gacuucucca 4920
aauuauucac cuacuucccc aagcuacagu ccaacauccc cuaacuauuc accaacaucu 4980
cccaacuauu cacccacuuc accuaguuau ccuucaacuu cgccagguua cagccccacu 5040
ucacgcagcu acucacccac aucuccuagu uacucaggaa cuucgcccuc uuauucacca 5100
acuucgccaa guuacucccc uacuucuccu aguuauucgc cgucgucucc uaauuacucu 5160
cccacuucuc caaauuacag ucccacuucu ccuaauuacu caccguccuc uccuagguac 5220
acgcccgguu cuccuaguuu uuccccaagu ucgaacaguu acucucccac aucuccucaa 5280
uauucuccaa caucuccaag uuauucgccu ucuucgccca aauauucacc aacuuccccc 5340
aauuauucgc caacaucucc aucauuuucu ggaggaaguc cacaauauuc acccacauca 5400
ccgaaauacu cuccaaccuc gcccaauuac acucugucga guccgcagca cacuccaaca 5460
gguagcaguc gauauucacc gucaacuucg aguuauucuc cuaauucgcc caauuauuca 5520
ccgacgucuc cacaauacuc cauccacagu acaaaauauu ccccugcaag uccuacauuc 5580
acacccacca guccuaguuu cucucccgcu ucacccgcau auucgccuca accuauguau 5640
ucaccuucuu cuccuaauua uucucccacu agucccaguc aagacacuga cuaa 5694
<210> 170
<211> 876
<212> RNA
<213> 玉米根萤叶甲属
<400> 170
augucgucaa auauucaaaa ggcccagcag uugauggcgg augcagaaaa gaaaguaaca 60
ucucgagguu ucuucggauc ucuauuuggg ggaucaaguc guauugaaga ugcaguggaa 120
uguuacacaa gagcugcaaa ccuuuuuaaa auggccaaga gcugggaugc ugccgguaaa 180
gccuuuugug aggcugcuaa uuugcauucc agaacuggug cucgucauga cgcugccacu 240
aauuauauag augcugcaaa uuguuacaaa aaagccgaug uauuugaggc uguaaacugc 300
uuuauaaaag cuauagacau uuauaccgaa augggucgcu uuacaauggc ugcaaaacac 360
caucagacua uugcagaaau guaugagacu gaugcugugg acaucgaaag ggcuguucaa 420
cacuaugaac aggcggcuga uuacuucaga ggagaagaaa gcaaugcuuc cgccaauaag 480
ugucuucuua aaguggcuca auaugcagcc caacuugaaa acuaugaaaa agcaguggga 540
auuuaucaag aaguggcuua ugcagcucug gaaagcucuc uuuuaaaaua cagugcaaag 600
gaauacuuau ucagagcugc ccuuugucac cuuuguguug auguacucaa ugcacaacau 660
gcuauagaaa gcuauauuuc aagguauccc gcauuucaag auucccguga auacaaacuu 720
uugaaaaccc ucauagaaaa caucgaagag caaaacguag auggauauac agaagccguc 780
aaagauuacg auucaauuuc ucgucuugau cagugguaua cuacaauucu uuuacguauu 840
aagaaacaag uaagcgaaag cccugacuua cguuaa 876
<210> 171
<211> 609
<212> RNA
<213> 玉米根萤叶甲属
<400> 171
augaaucccg aguaugauua uuuauucaaa cuucugcuga uuggagauuc aggaguagga 60
aaaucuuguc uucuacugag auuugcagau gauaccuaca cagaaagcua uauuaguacc 120
auuggcguag auuuuaaaau caggacaauc gauuuagaug gaaagacaau uaaauugcaa 180
auuugggaua cagcagguca ggaaagguuu agaacgauua caucaaguua uuaccgagga 240
gcacauggua uuauuguagu guacgauugc acagaccaag auucauucaa uaacguuaaa 300
caguggcucg aagaaaucga ccguuaugcg ugugacaaug uaaacaaauu acugguaggg 360
aauaaaagcg auuugacaac uaagaaaguu gucgacuuca cuacagccaa ggaguaugcc 420
gaccaauugg guauaccauu uuuggaaacc ucagcuaaga augcaaccaa uguagaacag 480
gccuuuauga cuauggccgc ugaaauaaaa aauagaguag gaccuccauc uucugcggua 540
gaccaaggaa auaagguuag guucgaucaa agucgcccag ucgaaacaac caaauccggu 600
ugcugcuga 609
<210> 172
<211> 789
<212> RNA
<213> 玉米根萤叶甲属
<400> 172
auggcagacg cugaugaucu auuagauuau gaagaugagg aacagacaga acaaaccgca 60
acugaaacgg caacuacaga gguacagaaa aaggguguca agggcacaua uguaucaaua 120
cacaguucug gguuuagaga uuuucuguua aaaccagcaa uucucagagc uauaguggac 180
ugcggguucg aacauccuuc agaaguucaa caugaaugua uuccucaagc ugucauuggc 240
auggauauuc ugugccaagc uaaauccggu augggaaaaa cggcuguuuu uguauuagcu 300
acacuccaag uaauagaucc uacagaaaau guuguauaug uucucgucau gugccauacc 360
agagaguuag ccuuccagau aagcaaagag uacgaacguu ucaguaaaua uaugcccaau 420
auuaaaguag gggucuucuu ugguggcuug ccuauccaga aagaugagga aacguuaaaa 480
aauaauugcc cgcauaucgu uguggguacu ccaggaagaa uuuuagcauu ggucagaucg 540
aaaaaacuua aucucaaaca ucuaaagcau uuuauuuugg augaauguga uaaaauguug 600
gaguuauuag acaugagacg ugauguucaa gaaauauauc guaacacucc ccacgaaaaa 660
caagucauga uguucagugc caccuuaagu aaagaaauua gaccaguuug caagaaauuu 720
augcaagaug uaauucaaaa uucuuauaau acacaauuuu guaaugacgc acccacucgc 780
aauguuuga 789
<210> 173
<211> 738
<212> RNA
<213> 玉米根萤叶甲属
<400> 173
augccgguca uugaugguua uaaaguacuu uacauuuuau uacacaguuu auauacaauu 60
uuugaaaaua uuuggaggac ucuuuuauuu auuuaucaaa auuguauaag gguuauaaac 120
ccugaaucua cauucgauga ugcugaccag uuaaagaaaa gacugucuag acuaacaaaa 180
aagccucaac auuuaacuau cauuauuggu guggaagaau auucauuggu agauuuggcu 240
aaccucguau auugguguuu aggucuuaau auuccguacg uuaguuucua ugauuauaaa 300
gguaauuuaa aaaagcauga agagaaguug caacaaauug uagaauccag aaaaucagag 360
aauaucaaca uaauuuggca cacccaugca gaacaaaggc auaaaaaugg auuuuugggu 420
ccaaaaaucc acguaaaagu guuaacacac gcggacggaa agcaaaguau aguaaauguu 480
acuaaaaaau uagcucuaaa uaaagaaaaa gacauuagua aagaaaaaau uagugaauua 540
cuauuaaggc aguaugaauu uccagaucca gaaauggcua uuauuugugg aaagaaacug 600
aacauuuaua auuauccucc uuggcaguua agacucacag aauucuuuaa agucaacaaa 660
gucaacaaca ucacauuccc aguguuugug gaaaaauugg aaaaguacag caaaugugaa 720
cagagggugg gaaaauaa 738
<210> 174
<211> 480
<212> RNA
<213> 玉米根萤叶甲属
<400> 174
augaccaacu cuaaagguua ccgccgagga accagggauc uauuugcccg caaguuuaaa 60
aaacguggug uaauuccacu uuccacauau uugagagucu acaaaguugg agauauugua 120
gauaucaagg guaauggugc aguucaaaag gguaugcccc acaaagugua ccaugguaag 180
acaggacgug uuuucaaugu uacugcacau gcauuaggug uaauuguaaa caaaaggguu 240
cgaggaagaa ucauccccaa aagaaucaau cuccguauug aacauguaaa ccacuccaag 300
ugucgucaag acuucuugca aagaguaaaa uccaacgaaa agcuacguaa agaagcuaaa 360
gaaaagaaca uuaaaguaga acuuaggaga caaccugccc aaccuaggcc agcacauauu 420
guuagcggaa agguuccagc acaggugcuu gcuccuaucc cauaugaauu cauugcuuag 480
<210> 175
<211> 537
<212> RNA
<213> 玉米根萤叶甲属
<400> 175
auggaaggaa uacuacugga accaacauug uauaccauaa aagguauugc uauauuggac 60
uaugauggua auagagugcu ggcuaaauac uacgauaaag auauauuucc uacagcaaaa 120
gagcagaaag cuuuugagaa aaauuuguuc aauaaaacuc auagggcaga cgcagaaauu 180
aucauguugg augguuuaac uuguguguau agaaguaaug uagauuuauu cuuuuauguu 240
augggcaguu cacaugaaaa ugagcuaauu uuaaugagug uuuuaaauug cuuguaugac 300
ucaguaaguc aaauauugaa gaaaaauaug caaaaacgag cugucuugga aucacuagau 360
auuguuaugc uggcuaugga ugaaauuguu gauggaggaa uaauuauaga uucugauuca 420
aguucaguag uaucuagaau agcauuaagg acugaugaua uuccauuagg agaacaaacu 480
guagcucagg uauuccaaac ggccaaagaa cagcugaaau ggucauugcu gaaauaa 537
<210> 176
<211> 450
<212> RNA
<213> 玉米根萤叶甲属
<400> 176
auggcugacc aacucaccga agaacaaauu gcugaauuca aagaagcuuu cucacuauuc 60
gauaaagaug gugaugguac aauuacgacu aaagaauuag gaacaguaau gagaucucua 120
ggacaaaauc caacagaggc ugaauuacag gauaugauca augaaguaga ugccgauggu 180
aacggcacga ucgauuuccc agaauuuuua acgaugaugg cacguaaaau gaaagauacc 240
gauagugagg aagaaauucg ugaagcauuc cgaguguucg acaaagacgg caaugguuuc 300
aucucagcag cagaauugcg ccacgucaug accaacuugg gugaaaaauu gacagacgaa 360
gaagucgaug aaaugauucg ggaggccgau aucgauggug auggucaagu caauuacgaa 420
gaguucguca ccaugaugac uucaaaguga 450
<210> 177
<211> 2013
<212> RNA
<213> 玉米根萤叶甲属
<400> 177
augaugcaag caaacaaucg agucccaccu auaaaguugg aaaacgauau agaucuuuac 60
gccgaugaua ucgaggauuu cgcacaagau gacuuuggug gugaaaaugu ugaucuauau 120
gacgauguaa uauccgcucc uccuggaaau aaugacaacc caggugauuc aaaucaucau 180
gcuccuccug gugcugguga agauggugga gguaauuuug uugggucagg aggagcaccc 240
aauaauauaa auucuucugg aagaagacau cagcuguaug uuggaaaucu gacuuggugg 300
acaacugauc aagauauaga aaaugcagug caugauauag ggguaaccga cuuccaugaa 360
guuaaguuuu uugaacacag agcaaauggu caauccaagg gauucugugu cauaucuuug 420
ggaucugagg gaagcaugag acucugccug gaacuccuau cuaaaaaaga gaucaauggc 480
caaaaucccc uuguuacccu ucccacaaaa caagcucuua guaacuuuga aagucagucu 540
aaaacacgcc cuucuccuac uaauaauucu aacucacguc cuccccaucc uaauaauaau 600
guucauucag guccuaugca gaauuaugga gguagaaugc cuaugaaccc uuccaugcgu 660
cccaugcccc cagguaugca aggugcucca agaaugcagg guccaccugg auuuaaugga 720
ccaccaaaca ugaaucagca accccccagg uuccaaggua auccacaaug gaauggaccu 780
agaccuaaug guccugggcc caauauggga augagaccca uggggccacc ucauggacaa 840
caagggcccc caagaccacc aaugcaggga ccaccgcagc aagguccucc aagaggaaug 900
ccgccacaag guccaccgca gaugcgucca gaauggaauc gaccaccaau gcaacaaggg 960
uacccucaag gcccgccgca uaugcaagga ccuaacaugg guccaagagg uccaccccaa 1020
augggaccac ccggggcgcc ucaacagcaa ggaccagcuc cgcacguaaa uccagcauuc 1080
uuucaacaag gaggaggacc accgccccca augcaacaca ugccuggacc agggcccguc 1140
augccuccuc aaggaccccc gcaaggucca ccacacggac ccguuggacc uccacacggc 1200
ccaccauugg guccagcgaa uguuccgccu cauggaccac cucacggaua ugguccaccu 1260
gcagcgaugc cacagccgcc auacgguggc ccaccuccag accaccgcgc ugagauuccu 1320
caguuaacag agcaagaguu ugaggauaua augucccgga auagaacagu uuccaguucg 1380
gcgauugggc gggccguauc cgacgccgca gcuggagaau uugcaagcgc cauugagacu 1440
uugguuacug cuauuucacu caucaaacaa uccaaagugg cuaacgacga ucguugcaag 1500
auccuuauaa guucgcugca agauacuuug cguggugucg aagacaaaag cuacagcucc 1560
agccgcagag accggucaag auccagggac agaucacaua gaagaacuag aagagaacga 1620
uccucgucac gguacagaga cagaagcaga gagagggagc gugaacgcga uagagaucgu 1680
gaucgugaac gugacagaua uuaugauaga uacagcgaaa gagaaagaga ccgagaucgu 1740
ucaagaagca gagaaagaac agaaagggau agagaacgag auuauagaga ccgggaaccc 1800
gaagagacag auaaagaaaa aucuaaagua uccagagucu caagaucaag aaacaaaucu 1860
ccggaaccug ucgaaccuag cagcgaggua ccgaaaucau cccgcuauua ugaggauagg 1920
uaucgggaac gagagagaga aggucgacga gagagcgauc gcgaaagaga aagagauaga 1980
agaggggaag acagccauag gucucgacac uag 2013
<210> 178
<211> 765
<212> RNA
<213> 玉米根萤叶甲属
<400> 178
augaguucua uuggaacugg guacgauuua ucagcuuccc aauucucucc ugauggaaga 60
guauuucaag uugaauaugc aaugaaagca guugaaaaua guggcaccgu aauaggccuc 120
cgagguacag auggcauugu auuggcugcu gaaaagcuca uuaugucaaa auugcaugaa 180
ccaaguacaa auaaacgaau uuucaacauu gauaaacaca uaggaauggc auuuucaggc 240
uuaauagcug augcaaggca aaucguugag auugcuagaa aagaagcauc aaauuauaga 300
caucaauaug guucaaauau uccucuuaaa uaccuaaaug auagaguaag cauguacaug 360
caugcauaca cuuuauacag ugcuguuaga ccauuugguu gcagugucau cuuggccagu 420
uaugaagaua gugacccauc uauguaucug auugauccau cuggaguuag cuauggauac 480
uuuggaugug cuacagguaa agcaaaacag ucugcaaaga cugaaauaga aaaauugaag 540
auggggaauc uaacaugcaa agaacuuguu aaagaagcag ccaaaaucau uuauuugguc 600
caugaugagc ugaaggauaa gaauuuugaa cuggaacuuu cauggguaug caaagauacg 660
aaugguuuac auaccaaagu gccugaauca guguuugcug augcagaaaa agcugccaaa 720
caagcaaugg aagcagauuc agaaucagau acagaagaua uguaa 765
<210> 179
<211> 744
<212> RNA
<213> 玉米根萤叶甲属
<400> 179
auggcuucaa aagacagauu gaugauuuuu ccaucuagag gagcccaaau gaugaugaaa 60
uccaggcuaa agggagccca aaagggacau aguuuauuaa agaagaaagc ugaugcuuua 120
caaaugagau uuagaaugau uuugaacaaa auuauugaga ccaaaacucu caugggugaa 180
guaaugaaag aagcugccuu uucuuuagcu gaagcaaagu uugcaacugg ugacuucaau 240
caaguuguuc uucaaaaugu caccaaggcu caaauaaaaa uaagaacuaa gaaagacaac 300
guugcuggug uuacuuuacc aguguuugaa ugcuaccaag augguacaga uacauaugag 360
uuggcugguu uggcuagggg aggucaacaa uugacaaaac ucaagaagaa uuaucaaagu 420
gcuguuaaac uguugguuga auuagccucu uugcaaacuu cuuuuguaac ucuugaugau 480
guaaucaaaa uaacaaacag aagagucaau gccauugaac auguuaucau uccaagaaua 540
gagcguacuu uggcuuacau cauauccgaa cuggacgagu uagaaagaga ggaguucuau 600
agauuaaaga agauccagga caaaaagaag aucagcagag caaaggccga gaaacaaaaa 660
caagcucuuc uccaagcugg gcuacuuaaa gagucccagg caaacaugcu uuuggaugag 720
ggcgaugaag aucuacuuuu cuag 744
<210> 180
<211> 4818
<212> RNA
<213> 玉米根萤叶甲属
<400> 180
auggaggcgg cucccaaguu accgaugcuc ucguucgagu uaaauacuug uacagagaac 60
guccacuuug gcccccaguu aaaacaguau auugcugcuu uuuaugguga agauccagaa 120
uccuacauua cagaaaucag caaucuugaa uccuuaagau cagcugcagu ucgaccauca 180
acggauguaa augguguaca acuguugaaa aaguauuucu gucagcuucg uuuucucaaa 240
ucuagguuuc ccauggaaga gaaucaagau gcugcaguuc uauuuucaug gaaaaauaau 300
gaauuagaca uaacuucaac auccagugau aucagauaug aauuaauggu aauaauguau 360
aacauuggag ccuuacacac uuuucuugga gccaacgacu caagaaacaa uccggauggu 420
augaaaaugg cauguacuca uuuucaaugu gcugcauggg cuuuucaaaa cguaaaagaa 480
aaguaccacc aauucauauc aaacaucuca uugguagaac ugguucauuu uuuucaacaa 540
gucuguuuag cucaggcuca ggaguguaua uuagagaaga gcauguuuga caauaggaaa 600
ccuaccauca uugcaaaagu ugcuauccaa gucuacaguu auuacagaca gucuuuacgu 660
gucuuggaau caguaaauga agccuacuuu agggauaaaa ccuacaagga guggaugaaa 720
uaucuucaau ucaagcugac cuacuacaaa ugcaucucgu uccuauucca agggcaacaa 780
gcugaggaac aacagaaaau gggagaaagg guugcauucu aucaagcugc augugaacag 840
cuggacgagg caaagaaaau ugcugcuaca uuaaaaaacc aacaccacca gcaagaaaua 900
aaugagggac uagcauucac uacugaugug guugaaggua aaagaaaagc agcuaaaaau 960
gaaaaugagu ucaucuacca ugaaucagug ccugauaaag accaauugcc agagguuaag 1020
ggugcuucau uagucaaagg aauaccauuc aguauaaaug auauagaagu uucaggacca 1080
gauauuuucu cccgauuggu cccaauggag gcacacgaag cagcuuccuu guacagcgag 1140
aagaaagcuc agagauuaag acagaucggg gaacuuauug aaaauaaaga ucaaacauug 1200
gcugaauuua ugucgucaau gcagcuagau cuauugacca agaugcacca ggcuacugga 1260
auaccgcagg aguugauuga uagagcagcg gcucuaucug cuaaaccuaa cgccauucaa 1320
gaucuuauaa gugcuauggg aaagcuaucu aauauauacc aagacguuga agcaaguuug 1380
aaugagauug auucuuuauu aaaggccgaa gaacaaagug aacaaaagua ccaagaaacg 1440
auugguaaaa gaccaccgag cauuuuagcu acagauuuaa cuagggaagc ggcaaaauac 1500
agggaggcuc auacuaaagc gaacgacuca aaccaaacuu uacacagggc gaugauggcu 1560
cacguggcua aucugaaaau acuccaacaa ccgcuaaagc agcugcaaca ucagcugccc 1620
uuugucgagu uuccaaaucc aaauaucgac gaaaaaucuu ugaaagaucu ggaagcgcua 1680
guugcaaaag uagacgaaau gagaacccaa agagccaugc uaugggcuca acuucgagaa 1740
ucuauucacc aagacgauau uacaaguucc cuuguaacga aacaaccaaa ucagucgcug 1800
gaacagcugu uccagcaaga acuucaaaag caucaaaauc ugauuucguu gauugaacaa 1860
aacaccucgg cacaagaaaa cauuaagagc gccuuagucg auucuuacgc uuacgcugua 1920
aauucaagaa aauacaucca agauauacuc caaaagagaa ccacaaccau aacgucacug 1980
auagcaucgu ucgacucuua cgaagacuua uuggcaaaag cuaacaaagg gauagaguuu 2040
uacucaaaac uugaaacgaa cguauccaag uuacugcaaa gaauaaggag uaccugcaaa 2100
guucaacaag aagagcgaga ucagaugaug ucgacugcgc aagugccuca augggagagu 2160
cauacgucac uugccgcucc uaaacugaaa gauuacuugg acuccaggaa gaagagugcu 2220
gcguauucgg agccgagugu ucaaccacaa cagccaacuu uaaguuacuc agcugcuaug 2280
gaucugccuc cugguauuag gccgacucca guuggaucag aaauaacgga uguaccgaaa 2340
aauauucaag gugaaccaca agguuauauu ccauauaauu accaacaacc uucuguuccu 2400
gccucacaga auauugauga agagacuauu aaaaaaauga acgcauugau gccaggugcu 2460
aagacgucag ugccuaguca guacggauac agcaacuaca uuccaccaac auacccucaa 2520
agugcguacc aaccagguaa ucagucuuac ggaaaagaaa cuccagauau uaacucaccg 2580
uacgacccua ccaaggcguu cacggcuacu acuaacgcuu aucguucggu gcagagcucc 2640
ucaacucaag gauacguacc guacgcagaa ucuaacguuu cgaauguuga cagaguugga 2700
uauccuagca gguaucagua ccaacaagua ccugagauag cuacuacucc agcugauccc 2760
aauauuaaug cguacuaccc acauggguac ucaccgagcc agaauuuacc gaaugcuaau 2820
acucaacaua uuaccggcca acugaaguac cauucggugg aguacgcuuc uucugugccg 2880
aacaacauca auuauaacag cucuaccuac ucgucgccgc uuucuaauau gucuaguacc 2940
aauuccucaa auccuaguaa cuugaauaau ucuuacgagu acuacuauga cccgaauacc 3000
aguaguggug caguaccgaa ugcuucaaag ccucaacagu cgagcgccag cucugcaaac 3060
ccgaguaccg cuaugaacaa cuacaauuau uacuacaaua caaguaccag cgguagugua 3120
gcagcggaua cuucaaaaau acaacaacaa caacaguacc cagguacuca gaugagucaa 3180
gcgcaguacu aucccgccaa ugccaguuau uacucaacca guacuuacaa uaccaacguc 3240
caagguggua ccaaucccuc guacgcaacu ggacaaacau auaaucaagu gacaccagug 3300
accucucaaa auguuucuca aaauuacaac uuuaaccaag uugguucugg agcaggacac 3360
cagcaucagu acuacucguc cgcuaacgcc gcaguaccau cccaacaagc uguaaauaac 3420
aguucauuac caaacuacgg auacgaucag uauuacggca acaacuauaa uuccagucaa 3480
ccgaguaccu acagcgcaaa ccaagcaccu ccugcagcac aagcugcucc aaguaauauu 3540
ccugcugcca ccaaauccuc cucuaaugug gaucugcuca guggcuugga cuucagcaua 3600
agccaagcuc cucuagugcc ucaacaaaac auuacgauaa aaccccaaga aaaggaaaca 3660
aaaccaccgg cuguuucuuc ugaaaccaaa aaccaagauc caacaccagu aaccacgccc 3720
aaacaaccca cuggaccaga aguaaagcgc uuguacguca aaauccugcc gagcaaaccc 3780
uuaaacaacg augaugugaa gaaauuguuc ggccaagagc uggacaggua ugagaaguuc 3840
guggagaccu ugacccacaa aacuuugagc gguccgacca cucuggauau uaaauggaag 3900
gagauccaag accagcagga uugcgagccg cagaagaaga ucauuuccgu cgcuagaugu 3960
uauccuauga agaauagguu cccggauauc uugccuuacg acuuuuccag gguggaguug 4020
ugcgauagua aagaugauua uaucaacgcu ucauacauua aggauaucuc gccauaugcu 4080
ccgucauuua uuguuacaca agugccguug ucuucaacug uuggugauau guggacgaug 4140
auuagagaac aacaggucga acugauccuc uguuugguaa acgacaauga gaucggugaa 4200
gauauuuacu ggcccaaaga aaaaggcagu agucuuaaca uacuuaacau ggucauaacg 4260
uugcaaaacg uuauaguuaa gucucauugg acugaaagac ugauagcgau aaacuuaccu 4320
gaaaaacggg agucccgugu gauaaugcau cuacaauuua caucguggcc uggcagcuug 4380
uuuccaacaa auccugaacc guucgucagc uacaccuugg aauccaucaa ccuauaccaa 4440
caacagaaga ccaacaccca uccgguggug guccauuguu caucuggcau aggaagaagc 4500
ggccugcucu guuuacugac agcugcuaug uucgaugcug ccaacaaugc uaacucgaua 4560
ccagaucuua cagcuuugag uaucaaguug uccaauugca ggaagaauau ucucagagau 4620
cgagagcauu ugaaguuugg uuacgaaagu uuuuuggcgu auauuaggca uauaguuugu 4680
gaagauaaag ccagaaagaa acugaacgag auccagccca agguuaagga ggaaccacug 4740
gaaccaccug ucauaguucc agaaccaaau auagauccuu uaaguacuuu agacccauuu 4800
ugggcuagua aaagauaa 4818
<210> 181
<211> 888
<212> RNA
<213> 玉米根萤叶甲属
<400> 181
auggcggacg augagagaaa gaaacuggag gaggaaaaga agaggaaaca ggccgaaauu 60
gaacgcaaaa gggccgaggu cagggcucgu auggaagagg ccucaaaagc caagaaggcc 120
aagaaagguu ucaugacccc ugagagaaag aagaaacuua gguuacuguu gagaaagaaa 180
gccgccgaag aauuaaagaa agaacaagaa cgcaaagcag ccgaaaggag gcguaucauu 240
gaagaaaggu gcgguaaacc caaacuuguc gaugacgcaa augaaggccc auuaaaacaa 300
guaugugagg gauaucacag acguauugua gaccuagaaa auaagaaauu ugaccucgaa 360
aaagaagugg aauucagaga uuuucagauc uccgaauuga acagccaagu aaacgaccuu 420
agaggcaaau ucgucaaacc aaccuugaag aagguaucca aauacgaaaa caaauucgcc 480
aaacuucaaa agaaggcagc ugaauuuaac uuccguaacc aacucaaagu ugucaagaag 540
aaagaauuca ccuuagaaga agaagacaaa gaaaagaaac cagacugguc aaagaaggga 600
gacgaaaaga agguacaaga ggcugaagca ugauuuuucu ccuuuguuaa agcccuuuug 660
ucaacaucaa gggauauguc guuauuucga ugaucccauc gugauuucga uaucuuaaau 720
auauuuauuu uauucauuac uuuccagacu aaaagagugu cuguccgcau guauauuauu 780
uguuuaugua uaacuuauua aaaaauguga aguauuguaa aaaaaaaaaa aaaaaaaaaa 840
aaaaaaaaaa aaaaaaaaaa aaaaaaaaga aaaaaaaaaa aaaaaaaa 888
<210> 182
<211> 791
<212> RNA
<213> 玉米根萤叶甲属
<400> 182
ccgugaucuc gagcgguuuu uaacagagag ggaaaaugua aucauauaua ugguugaauu 60
cuugaagguu agacuucauu ccagucuugu gauauuuagu gcuuacuugg uacagcaguu 120
ucagugcugu gcuuuagaau aauuuauuuu uuaacauuua uauagaaauc aaauacuaac 180
caaucaacau gugugaagaa gaaguugccg cuuuagucgu agacaaugga uccgguaugu 240
gcaaagcugg uuuugcuggg gaugaugcac cucgugcugu auucccuuca auuguuggac 300
gcccaagaca ucagggugug augguaggaa ugggacaaaa agauuccuau guaggugaug 360
aagcucaaag uaaaagaggu auccuuaccu uaaaauaccc caucgagcac ggaauaguca 420
caaacuggga ugauauggag aaaauuuggc aucauacauu cuacaaugaa cucagaguag 480
ccccagaaga acacccuguu cuguugacag aagcuccucu caaccccaag gccaacaggg 540
aaaagaugac acaaauaaug uuugaaacuu ucaacacccc agccauguau guugccaucc 600
aggcuguacu cuccuuguau gcaucugguc guacaacugg uauuguguug gauucuggug 660
augguguauc ccacacuguc ccaaucuaug aagguuaugc ucuuccucau gcaauccuuc 720
guuuggacuu agcugguaga gacuugacug auuaccucau gaaaauuuug acugaacgug 780
gcuacucuuu c 791
<210> 183
<211> 2927
<212> RNA
<213> 玉米根萤叶甲属
<400> 183
uaauuuuuga caguucgaaa auggugaugu guuuauaauu cgugucaauu uagugaaaua 60
gcauucaauu uuuuguauaa gaucucuuca aaaugccacu ucgauuagau auaaaaagaa 120
agcuaacagc ucgcucagac cggguaaaau guguggaucu ucacccuaca gaaccuugga 180
ugcuguguuc ucuuuacagc ggaaauauaa acguuuggaa caccgaaaau cagcaacugg 240
uuaagacuuu ugaaguaugu gauguaccug uucggacagc uaaguuuuug cccaggaaga 300
acuggauagu cagugggucu gaugauaugc agauucgagu uuucaauuac aauaccuuag 360
aucggguaca uucuuuugag gcucauucgg auuaugugag auguauuguc guacacccua 420
cacaaccuua uauauuaaca aguagugaug auaugcuuau caagcuuugg aauugggaaa 480
aagcaugggc uugucagcaa guuuucgaag gacacacuca uuauauuaug caaaucgcca 540
uaaauccaaa agacaacaac acauuugcca gugcaucccu agauagaaca uugaaaguau 600
ggcaauuggg agcguccaca gcgaauuuca cacuagaagg ucaugagaaa ggcguuaacu 660
guguggacua uuaucacggu ggagauaaac cuuauuuaau cucaggcgcu gaugauagau 720
uaguaaaaau cugggauuau caaaacaaaa cuuguguuca aacuuuggaa ggacaugcuc 780
aaaauguaac cgcugcaugu uuccauccag aacuuccugu agcucuuacu ggaagugaag 840
augguacugu cagagugugg caugccaaca cccauagguu agaaaguagc uuaaauuaug 900
gcuuugaaag aguauggacu auuuucugcc uaaagggauc caauaacgug gcauuggguu 960
augaugaagg uagcauuuug guuaaaguug guagagaaga accagcuguu aguauggaug 1020
ccaguggagg caaaauuauu ugggccagac acucugaacu ucaacaggca aaucucaagg 1080
cguuagcuga aggugcggaa auaagagaug gagaacgccu uccaguuucu guaaaagaua 1140
ugggugcuug cgagauauac ccucagacaa uucaacacaa ucccaauggc cguuuuguug 1200
uugucugugg ggauggagaa uacauaaucu acacagcaau ggcuuuaaga aacaaagcgu 1260
uugguagcgc acaagaauuu gugugggcuc aagauuccag cgaauaugcc aucagagaau 1320
ccggaucuac uaucagaauu uuuaagaauu ucaaagagaa gaagaauuuu aaguccgauu 1380
uuggagcuga agguauauac gguggauacc uuuugggagu caaaucgguu ucugguuuga 1440
cuuucuauga uugggaaacu cucgauuuag ucagaagaau cgagauacaa ccaaaagcag 1500
uuuacugguc agauaguggu aaauuaguau guuuggccac agaagauagc uacuuuauuc 1560
uuucuuauga uucugaugaa guucaaaaag ccagagauaa caaucagguu gcggaugaug 1620
gaguagaauc ggcuuucaau cuucuaggug aaauaaacga aucagugcga acuggucucu 1680
ggguaggcga cuguuuuauc uacacgaauu cuguuaaucg uaucaacuac uucguuggag 1740
gugaacuggu uacaauugcu cauuuggacc ggccuuugua ugucuuggga uaugugccua 1800
aagacgauag auuauaccuc guagauaaag aguugcgcgu aguaagcuac caauuacuuc 1860
uuucuguucu ugaauaucaa acugccguca ugagaagaga cuuuccaaca gcagacagag 1920
uacuuccguc cauuccuaag gagcacagaa cgagaguggc acauuucuua gaaaagcaag 1980
gcuucaaaca gcaagcuuug gccguaagua cagauccaga gcacagauuc gagcuggcag 2040
uagcauuaga ggaucuuaau auagccaaaa cucuagcuca agaagcgaac aguccgcaaa 2100
aguggaauca acuagcagaa uuggcagcug cuacuaauaa uguaagcgua gccaaggaau 2160
guaugcaaaa agcgcaagau uauggaggcu uguugcuucu ugcuacgagc uccggugaug 2220
aaaauuuagu ccguacucua ggagaaacga cacaagcuga aagcaaacau aacuuagcau 2280
uuuugucaca cuuguuagua ggugauuuaa acaaaugucu agacauucuu auuaauaccg 2340
guagauugcc agaagcugca uuuuucgcca gaucuuaccu uccugauaag auuacagaag 2400
ucguggaacu guggaagacu caguuaucuu cagucaauca aaaagcugga cagagccuug 2460
ccgauccuaa aaacuacgaa aaucuguucc cugguuuaca agaggcggug guagcucaga 2520
aauuuuugga acagcagaau aaagguuuag cgcccgcaag aguugccacc accauuccuc 2580
cuaaucacga caggaauguu guagccgaag uucaagcaca aucgaaacac gauguaccau 2640
cauuuaguuc uucguuuauu ucaucagaaa uagaagcaca aacaaggagu ucugcuaaac 2700
cugaagaauc uucaaacauu auacagcugg accaagauga cgacgauauc gauuuagauu 2760
uggacggugu aaauaucgau gagaacauug acacgacgga uaucaacauc gaugaugauu 2820
ugcugaguga uugaaaauaa cuuuuuuacu uuaguauuaa aucuguauau ucauuccuau 2880
ucuuaagaaa aucuauauga auuuuaaugu uuuaauaguu aagaaau 2927
<210> 184
<211> 1620
<212> RNA
<213> 玉米根萤叶甲属
<400> 184
uucauuucgc cauuuugauu auuucaauuu uagaaaacgu ucaauaagca gggugcaggg 60
ucaagaagua aaguguucua gaaauacaga uuaaauuguu uccuuugugu uauugaaagg 120
caaaaauaaa auaaugcaga ucuuuguaaa aacacucacu gguaaaacca ucacccucga 180
gguugaacca ucagauacca ucgagaaugu caaagcuaaa auucaagaca aagaagguau 240
uccaccagau caacagagau uaaucuuuag aaagcguucg aguucgauaa gcaagcaggu 300
caagaaguaa aguguucuag aaauacagau uaaauuguuu ccuuuguguu auugaaaggc 360
aaaaaucaaa uaaugcagau cuuuguaaaa acacucacug guaaaaccau cacccucgag 420
guugaaccau cagauaccau cgagaauguc aaagcuaaaa uucaagacaa agaagguauu 480
ccaccagauc aacagagauu aaucuuugcu ggaaagcagu uagaagaugg ccguacucuc 540
ucagacuaca acauucagaa agaaucuaca cuacacuuag ugcuucgucu uagaggaggu 600
augcacaucu uuguaaaaac ucucacuggu aagaccauca cccuugaggu ugaaccauca 660
gauaccaucg agaaugucaa agcuaaaauu caagacaaag aagguauucc accagaucaa 720
cagagauuaa ucuuugcugg aaagcaguug gaagauggcc guacucucuc agacuacaac 780
auucaaaaag agucuacccu ccauuuggua cuucgucuua gaggagguau gcagauuuuu 840
guuaaaacuu uaacuggaaa gaccaucacc cuugaaguag aaccuucuga uaccaucgaa 900
aaugucaaag ccaaaauuca agacaaagaa gguauuccac cagaucaaca aagauuaauc 960
uuugccggaa agcaauugga agauggucgu acacucucag acuacaacau ucaaaaggaa 1020
ucuacccucc auuugguacu ucgucuuaga ggagguaugc aaaucuuugu aaaaacacuc 1080
acugguaaga ccaucacccu cgagguugaa ccaucagaua ccaucgagaa ugucaaagcu 1140
aaaauucaag acaaagaagg uauuccacca gaucaacaga gauuaaucuu cgcuggaaag 1200
caguuggaag auggccguac ucucucagac uacaauauuc agaaagaguc uacccuccau 1260
uugguacuuc gucuuagagg agguaugcaa aucuuuguaa aaacucucac ugguaagacc 1320
aucacccucg agguugaacc aucagauacc aucgagaaug ucaaagcuaa aauucaagac 1380
aaagaaggua uuccaccaga ucaacaaaga uuaaucuuug ccggaaagca guuggaagau 1440
ggccguacuc ucucagacua caacauucaa aaagagucua cccuucacuu gguacuucgu 1500
uuaagaggag gaaauuaaua ugguugaaau uaagcacauu uuuauauuuu caauaaauaa 1560
auuauaaauu auuaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1620
<210> 185
<211> 2430
<212> RNA
<213> 玉米根萤叶甲属
<400> 185
cucgaaacaa augacuguga augaaaagcc cuauagcaac agcaacaaca aauauuugaa 60
uuuucgguaa auaacguuua ugaaaaauaa uaauaauuuu aauuguuaca uaagacacau 120
aagaguacau aagauuguau uauggaagaa cgagauuuag uuucuaauag acgcagauca 180
uugcauaacu auggcgaaga gaaaccuguu ugucgaaaau uuccggauaa ugaugaacaa 240
aauuuccaau auccuccaga uuauauccac aaaguaaaua cagaaacgga aaaauggaau 300
gcucaccuca guaacaucuu agucgaccaa cagagguaca aagaaaaaau acgugaacuu 360
aaaacuguag uguacaauga aaacguuuug ucacaugauc agcaggaauu uuuaaaaucu 420
auagacuuua auacauacuu gagacaaacu gaaauauuuu guaaaaaggu gcauuuagcc 480
gcagaacuau acaguuucaa uaaaagugaa aaauaucaag aguuacagaa aaccauugaa 540
cauguucaag aaaucauuga uaguaaacug aagacauuua aaggucgcug uaccaccaaa 600
ccaaaaaacc auaaaucaug ugugacgacg auguagcggc ucuugucguc gacaauggcu 660
ccggaaugug caaagccggu uucgccggug augacgcccc ucgugcuguc uuuccaucca 720
ucguaggucg ucccagacac caagguguca ugguggguau gggucaaaaa gacuccuacg 780
uaggagacga agcccaaagc aaaagaggua uccucaccuu aaaauacccc auugaacacg 840
gaauuaucac uaacugggac gauauggaaa agaucuggca ucacaccuuc uacaaugaac 900
uuagaguagc ccccgaagaa caucccauuc uuuugacuga agcuccacuu aacccaaaag 960
ccaacagaga aaagaugacu caaaucaugu uugaaacuuu caauaccccu gccauguaug 1020
uugccauuca agcuguauug ucucuguacg cuuccggucg uaccacuggu auuguacuug 1080
auucuggaga ugguguaucc cacacaguac ccaucuauga agguuacgcu cucccacacg 1140
ccaucuugcg uuuggacuug gccgguagag acuugacuga cuaccuuaug aagaucuuaa 1200
ccgaaagagg uuacucuuuc accaccacag cugaaagaga aauaguucgu gacaucaagg 1260
aaaaauugug cuauguagcu uuggacuucg aacaggaaau ggccacagca gccagcucca 1320
ccuccuuaga aaagaguuau gaacuuccug acggucaagu caucaccauu gguaaugaaa 1380
gguuccguug cccugaagcu cucuuccaac cuuccuucuu ggguauggaa ucuugcggua 1440
uccacgaaac ugucuacaac uccaucauga agugcgaugu cgacauccgu aaagacuugu 1500
acgccaacac uguccuuucu ggagguacca caauguaccc ugguauugcc gaucguaugc 1560
aaaaggaaau cacugccuug gcuccaucaa ccaucaaaau caagaucauc gcucccccag 1620
aaagaaagua cuccguuugg aucgguggcu ccaucuuggc cucccucucc accuuccaac 1680
agauguggau cuccaaacaa gaauacgacg aauccggccc uggaauuguu caccgcaaau 1740
gcuucuaaac uacuuuauau auuuaucgua uacauauuaa guacaauacu gagaguugga 1800
gcaugaaugu auguuuuuau uuaugguuau auauaugaug acuuguugau auuguaacaa 1860
uaaauucauu uuguauuacu cugguaauau uuuauuuaug agaacaacca gauugaaguc 1920
guaaagagcc aauaacaacc ugaagauauc aauugucaau ugcuacugag uaauaguuug 1980
agguacuuag cuccagcucc uuuucacacg aaaguaagaa guaucugagc gagaauacau 2040
ucuguauugu acuuuuuaaa uaugcgacuu uuguaacauc aauuucauug uaaaauauca 2100
ucaucauugu uuuuauggac cuacgugaca guaggagaca ccugacaaga cuucuuuucc 2160
ugcuguguau acgcaccuuu acaaacaucc ugcuggccua auuguagucc uguaagggac 2220
auccuuuguu gggcauggag uuuguuugug ggaauuguau ugacuacuau uauauaccua 2280
cuuuuauuau uaaggcauuu gaaaucguaa acuaaaauug guuguuuaua uuuuauauga 2340
guauuuuuag uaguagauaa gauuucaauu gcaaaacuac cuauguaugu auucauuaga 2400
aauaaauucu uauuccaaaa uaaaguuuug 2430
<210> 186
<211> 2822
<212> RNA
<213> 玉米根萤叶甲属
<400> 186
guuuauuuug gucgucggcu gaugugacgu guaaagaaau uaaaucaaau uauuuuaaag 60
uuuuuaaauu aaaaugggua cuuuuaaaag agauacucau gaugaggacg ggggaucaag 120
ugcuuuucaa aaucuggaga aaacuacugu uuugcaggaa gcuagaguuu uuaaugaaac 180
uaguguaaau ccaagaaaau guacaccgau acuaacaaaa cuguuguacu uauugaacca 240
gggugaaacu uuaagugcca aagaggccac agauguuuuc uuugccauga ccaaacuguu 300
ccaaucaaaa gauguaauau ugagaaggau gguuuauuug ggaauuaaag aacucaguuc 360
uguugcugau gaugucauua uuguaacauc cagucuuaca aaagauauga cugguaaaga 420
agacauguac agagcagcug cuauaagagc auuaugcagu auuacugaug cuacuaugcu 480
ucaagcuaua gaacguuaua ugaagcaagc uauuguagau agaaacgcag cugucaguuc 540
agcagcacua auuaguucau uacauaugag caaauuagcu ccagauguag uaaaaagaug 600
gguaaaugaa gcucaggaag caguaaauag ugauaaugca augguacagu aucacgcauu 660
aggucuucua uaccauauua ggaagacuga uaagcuagca gugacaaaau ugauuuccaa 720
gcugaauuca auggguuuaa agagcccuua ugcuuugugu auguugauaa gaaucacugc 780
aaaacuuuua gaagaagagg accaagaguc acuccucaac uccccauaua caauaauauu 840
uacaaugggc uuaaggaaca aaucugaaau ggugguguau gaagcugcac augccauggu 900
uaaccugaag uucacgagua guaaugugcu agcacccgcu auaaguguuc uacaacuauu 960
uuguggaucu ccuaaagcca cacucagauu ugcugcuguu agaacuuuaa aucaaguggc 1020
caccacccac ccugcgucag ugacagcuug uaauuuggau cuagaaaauu ugauuacuga 1080
uccuaauagg ucaauugcua cacuggccau uacuacucuu uugaaaacag gugccgaauc 1140
uucuguugac agacuaauga aacaaaucgc uacuuuugua ucugaaauca gugaugaauu 1200
uaaagugguu gucauucagg caauuaaggu auuagcuuug aaauuuccaa ggaaacauag 1260
cacgcuuaug aauuuccuau ccgccauguu aagagaugag ggagguuuag aauauaaagc 1320
auccauagca gauaccauua uaacccuaau cgaagauaau cccgaagcua aagaaucugg 1380
uuuggcgcau cuuugcgagu ucauugaaga cugugaacau guuucuuugg cugugagaau 1440
cuugcauuug uuaggaaagg aaggacccaa gaccaaacaa ccaucgagau acauccguuu 1500
uaucuacaau cgcgucauau uggaaugucc uucuguaaga gcugcugcag ucuccgccau 1560
ggcacaauuc ggagccucuu gucccgauuu guuagaaaau auccaaauau uacuuucgag 1620
gugucagaug gauucagacg augaaguuag ggacagagcu acauauuaua guaauauacu 1680
uaacaaaaau gauaaaaguu uauacaacaa uuacauuuug gauucuuugc agguuucaau 1740
uccuucacua gaaagaucgc uuagagaaua cauucaaaau ccaacugacg aaccauuuga 1800
cauuaagucc guaccuguag cagcagugcc aacagcagaa gaacgagaag uuaaaaacaa 1860
aucugaagga cugcuagucu cucaaggucc aguccgaccu ccuccggugu cuagagaaga 1920
aaacuucgcc gaaaaacuua guaacguucc ggguauacaa caguuaggac cuuuguucaa 1980
aacuuccgac gucguugaac ucacugaauc ugaaacagag uauuuugucc gcuguaucaa 2040
gcacuguuuc aaacaucaca ucguccucca auucgauugu cugaauaccu ugccagacca 2100
gcuuuuagaa aacguuagag uggagauaga cgccggugaa accuucgaaa uuuuggcaga 2160
aauaccuugu gaaaaguugc acuauaacga aaccgguacc acauauguag uaguuaaguu 2220
gccugaugau gaucucccca acucuguugg uacgugugga gccguguuga aguucuuagu 2280
gaaagauugu gauccaucaa cgggaauacc agauucugau gaggguuacg augaugaaua 2340
uacacuggaa gacaucgaaa uaacauuagg ggaccaaauu caaaaaguaa gcaaaguaaa 2400
uugggcugca gccugggaag aagcugcagc uacuuaugua gaaaaagagg auacauacuc 2460
cuugaccauc aauacgcuaa guggcgcugu uaagaauauu auucaguucu ugggauuaca 2520
gccugcggaa aggacugaca gaguaccgga ggguaaaucu acgcacacau uacuucuugc 2580
ugguguauuc aggggaggua uugacauacu aguaagagcg aaacuagcuu ugggcgaaug 2640
uguuacgaug caacuaacag ucaggucgcc agauccugac guugcugagc uuauaacuuc 2700
aacuguaggu uaaguuuaaa ggcuacguua augauuauau uguauuacaa uuuuuccaua 2760
uguauaaaua uuuugauuua uuuaaauuuu auuagaaauu aaacaauuuu aaguaaaaaa 2820
aa 2822
<210> 187
<211> 3701
<212> RNA
<213> 玉米根萤叶甲属
<400> 187
gucguuuagu agaaguguca uuuuugucug cucucguuuu caucuuuugu guauaaaauu 60
ggugcuaagg uagauaaacu auauucgaau aggcguuuuu augucgcaag aaaguguuuu 120
guauauuugu gauacgugau cucguagagg gaguccagaa auucauaauu uauaguuaag 180
uaaaaaaugg cggcaaacag aacuggaccu gcucagagac caaauggcgc uacccaagga 240
aagauauguc aguucaaacu gguccuacua ggcgaaagug ccgucgguaa gucgaguuug 300
guacugaggu ucgucaaagg acaguuccac gaauaccagg agaguaccau aggagcagcu 360
uuccuuacac aaaccauaug ccucgacgau acaacuguua aauuugaaau uugggacaca 420
gcgggucaag aaagguacca caguuuagcu ccuauguacu auaggggcgc acaggcagcu 480
auagucgucu acgacauaac caaucaagac acauucggca gggcgaaaac gugggugaag 540
gaacuucaaa ggcaggccag uccgacgauc gugauagcuu uggccggcaa caagcaagau 600
uuggccaaca aacguauggu agaauacgaa gaggcgcaga cguaugcuga cgaaaacggc 660
uuacuuuuua uggaaacuuc cgcaaagacg gcaaugaacg ucaacgauau auuuuuagca 720
auagcuaaga aacugcccaa gaaugaacaa accacagguc aaggcggcag ugcccaaggc 780
aggcggcuag cggagggcga uucgggcgcc aaggcacccg gaaauuguug caagugaugg 840
uauacgccug caggucgagu guuguuauua aaccgucacg agaaaggacu ggcaagugca 900
gcggcacucu agugauaucu auguguaaua aagguccuuc uauuaacaaa aaaaaauuaa 960
uaaaaaaaua uauuaaacuu cauauacacu gucacauauu ccauuuagau gaugaaaaac 1020
aaaagagcag aagcauuuug gucucuaacg gucauguuga guuggaaugu cucguggggc 1080
auuuaaauuu gugauaauug uaccuauauu uuguuuuuuu uauauauaua auuaauauau 1140
cugacagugu aaguuaagcg uaaacuguuu auaucgaucg uauuagcagc accaauuaaa 1200
aaaauaaaua aaauugaaga ucuuuuuuau uguuuuugua auuuuaacuc uuuggggaag 1260
guuacaaaag aauugauaac guuguguuga aaacauugca uuauaauaaa guaauuccac 1320
ccacuuacga auuauuuuga aaggagaauc aaaaaugugu caauugucac cugucaucuu 1380
augaugacac ucuaugagag ugggggcaau ugcagcgcgc gcauacuauu cagucuagcu 1440
ucauuauguc aauuaaaugu cgauguuuaa aauuucaucg auuaguuuga caguucccaa 1500
augaaaaaac aguuacuucc gcauaaaaau guauugcuuc aaaacuuacu uucaguggca 1560
uuucaguggg uuuuagccau cuuauggaga guggucuaac acacuuucua gguacagcug 1620
ugguuuuuaa aaggaaauau aaugaaucgc uuuuguuuuc gaaauuuaca uugggaaucc 1680
cuuagugucc uuauauuugc ccuacagagu uucauuuaaa cugaaauugg guaauuuaca 1740
uagaccuaaa gcuuucacgu cuauauagcc uaauuagacc acaaaauuga cuugcuuuac 1800
gugaaaugua ugaaccauuc aaacauauuu auaugacaac aauauaauau aauacaauau 1860
aauauaaaua acugacaaca uuuaaaauua gaauuuuuac ccacaauuuu ucucugccau 1920
gugucaucua guacaagaag uauggcaauu uagcccuacu ccucuuauug uacauuucuu 1980
guaauuccuc auuuucuuuu ucaauuacuu cgaucuuuua aacuuaauuc accaaguuua 2040
uuugcaauau uguucuugaa gucuauaguu ugaauuccuu caauauauuu guaauguuuu 2100
ucuucagugu caauccuugc auuccaugaa agaacacgaa aaagaugcag cacuuuauuu 2160
guccauauuc cugcuacaua guuauuuuuc gauuaacaca uucacugcca gcguauagua 2220
aacuggcacu uuacuauacg cuaaacaaaa auuuggccuu gcagauuuuc cuaaaacacg 2280
uagcagcuac gccacugaau ggacuuagga cccccuaucg uuucgcuggu ugcaauaguc 2340
cgguaacuag agaagcaauu auuauauaaa aguaaauuua aauaauugua uuuugcuugc 2400
aguacugcau uuuaauaauu aguuuucuuu acuacauaca auuguuaccu uuuaauaaca 2460
uaaucuaaau cuuacuuuuu uuuuuuuauu uuuuugggcu auggccuuga caauuaucca 2520
guaaccagga cuaauauaau uggccaauau aauuaaaagu gcgaauaaua guacagagcg 2580
uagaaauagg ggucgcuuug ccgaacuugc acggucccaa uaguaaacug gcacuuuacu 2640
auacgcuaaa caaaaauuug gccuugcaua uuuuccuaaa acacguagca gcuacgccac 2700
ugaauggacu uaggaccccc uaucguuucg cugguugcaa uaguccggua acuagagaca 2760
aaaguggguc auuuugcaca cuucauauaa uucucguuuu cuguacuuca cgaguauuuu 2820
uuuugucgga uauguauuga auaguaucgg ugacaacaca cugcccugcg ugacaccccu 2880
uucaaaaccu augugggaac aauauguauc uuuagagaac uuuuuguacc cuuccccuaa 2940
aaacuaaaua uuuuuucacu uagugcaauu uuaauauuag ucucaccuua uuucuagaau 3000
acggucgguc uaaauguaua aucucaacuu ucagcuuaua cuucgaaaac cgagaaguau 3060
aaacuguaag aauauuuaac guuauaugua uuuuauuagg auaaugauug uugaaaguau 3120
guuucccaac acguguaagu uacauuucgu acucuuucca uuuuuauuac aaaaaaaaau 3180
guuuauuuag auaaacuguu gcggaacaua cuuucucgau gcaugacuga aaagaacguu 3240
aacauuuaua cguauuuguu uuuauuuugu gucugaugug acaacccaga ucuuagcgaa 3300
agcguagagg acaaaagaug aguuuuuaag uuucccauug aacuuuuucc guccuuaaau 3360
caaauacaac aacuauucac uuaaguuuuc uaaacauuuu ugcuaauaug ggccccugca 3420
ccauucccga cuuaacaguc uucauuuguu uuuauuaggu uuguucguag uacgauccaa 3480
acgaucggca accguugcca accauuagac gcaugcgcag uuaacaguga gugaucguag 3540
acuacgaaca cgcaugcguc ugaugguugg caacgguugc ugaucguucu acgaacaaac 3600
cuauuauaaa ccgccucuuu uguagguaaa guaaaccuua uaaucauuuu ccgagucuac 3660
aauuucugua ggacaucaag cauuguaaag uuuaaugaaa u 3701
<210> 188
<211> 671
<212> RNA
<213> 玉米根萤叶甲属
<400> 188
cugcgauaua gguguguauu ucagcuggaa uuuguaauga aaaaaacgua gaaauauaua 60
cuacaaugaa guuuuuaaga ucgacagugu gcuacauugc caucuuggca auucucuuua 120
cccucugugc cgaugagguu gaaggaagga gaaaaauuuu gauggggcga aaaagcauua 180
ccaggacaua ucuucgugga aaugcuguuc cugcguaugu gauaauaauc cuuguaggaa 240
uuggucaaau cauccuggga gggauauugu acguugcauu gaggaagaag aucauugcug 300
caccuguaac ggcaucauau gcaguggcua gacaagaacc auaaauuuua uuugucuaga 360
auauuauuuu cuaaauaugc aucuuuuuua aauuauuguc uacguaaaua auaagucuag 420
aaauauauaa aaauuguaua aaaucaugua ccuauauuuu ucaauuuuua uaaaaaacaa 480
cccgaaauuu aauauuuuac ugaauuaaca uuaucauuuc uaucuacacu caccggcaca 540
aaauuccguc acccaaaauu uuugauuaag cuugacaauu uauaacuaua uuauuugugc 600
uccgauuuuc aacuucaugu ugugcaucag uuuguaggua cauguauuga uauuguuugg 660
uauuauuccg g 671
<210> 189
<211> 693
<212> RNA
<213> 玉米根萤叶甲属
<400> 189
guagucggua uguauuugaa uuuaucuuaa uuuguuuaau auuaguaguu aaauauuaaa 60
cuauaguuag aaguuguuau auaguggacc aguaguugac uccccaaaau gcagaucuuc 120
guuaaaaccu uaacggguaa gaccaucacu cuugaggucg agcccucaga uacuaucgaa 180
aaugugaaag cuaaaaucca ggauaaagaa ggaauucccc cagaccagca acgucucauc 240
uucgcuggaa aacaacucga agauggucgu accuugucug acuauaauau ucaaaaagaa 300
ucaacccuuc acuugguguu gagauugaga ggaggugcua agaaacguaa gaagaagaau 360
uacuccaccc ccaagaaaau caagcacaag aagaagaagg uuaaguuagc uguauugaaa 420
uuuuauaagg uugacgaaaa ugguaaaauc caccgauuga gacgugaaug ccccgcugaa 480
caauguggag cuggugucuu cauggcagcc auggaagaca ggcauuacug uggcaagugc 540
gguuacacuc uugucuucuc caaaccagga gaugagaaau agauauaugu ccuuguauau 600
uguuuaagaa aaaaauagaa aaaccuuugu uuauuugaau aaaauauucg aggaagaaaa 660
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa 693
<210> 190
<211> 2684
<212> RNA
<213> 玉米根萤叶甲属
<400> 190
gaauuuaauu ucaaagauuu auguucagua agcuuuguuu caaagugcgg uacuaucggu 60
uucauuuuau aucucuuuau aacggugucg cgaguuuucu ugugaaaaau gauguccaaa 120
gcagacacac aggaagaugc cuccuucgcc aaauuggaaa aucagauugc uaucaucaaa 180
uacguaauac ucuuuaccaa cguuuugcaa ugggcucucg gugcagcaau cuucgcucuu 240
ugccuuuggc uacgauucga ggagggcauu caagaauggc uccagaaauu ggauucagaa 300
caauuuuaca ucggaguaua uguacuuaua gucgcuucac ugaucgucau gauugugucc 360
uuuauaggau guauuagugc ccugcaggag aguaccaugg cccuuuuagu guacaucggc 420
acccaagugc ucaguuuuau auucgguuua uccgguucgg cgguucuucu ggauaacagc 480
gccagagauu cccacuucca accgaggauc cgagagagua ugcgacgucu uaucaugaau 540
gcucaucacg accaauccag acaaacacua gccaugauuc aggaaaaugu ugguugcugc 600
ggagcugaug gcgcaacaga cuaccucucu cuucagcagc cccuuccaag ucagugcaga 660
gacaccguua cuggaaaccc auucuuccac ggauguguag augaacucac cugguucuuc 720
gaagaaaaau gugguuggau agcagguuua gcuauggcga uaugcaugau uaacguccuu 780
aguauuguuu uaucuacggu acucauccag gcauugaaaa aagaagaaga agcauccgau 840
ucauacagga gauagauuua gugagauaga gauauaaugu aguaauuaga auuuaaugua 900
ucuucaacua aauuacuuuu ucuuuagaga uauaccugaa auuguaaaga acaggaaaau 960
uaaauaagaa ccaaaaacua aagugaacca acaauaauug aacauuccaa aauacacuuu 1020
uuuuguuaag uuaacuaaac gacauaaauu uuucauuuuu uaaguuuuuu auuguuuuuu 1080
uuaguauuau aauuuggaua agguguuuuu auauuaagug uguaauuaua aaguuuuuuu 1140
auaggacgga accuaaauua uauagaauca uacaauaaac uauugucugc uuauugaauu 1200
uggaaaauaa acauuuggua uauauuaaaa auaauaauau augucuuaau gaggaacuaa 1260
ugaaaacguc uauacauuuu ugaauuuaau accaacagau auuguaauua uuaauuuuaa 1320
uuaaucaacu ccaagucaac aucuggaaag caauagaaau uaaaguaauu aacuaacuag 1380
uaacauucua gcaaccugua caugugguug uauuacucug uuuugacauu gacaaaacua 1440
gcuuugugau caguuaucuc uagcaguaau aaacucuagc uguauuuugu uuuauauauu 1500
uguccaaaga auugguuuau uuuaaagcaa auauaauggu uuaacccagg ggugggcaaa 1560
cuuuuuuugg uaagggccau aaaauauuuu ugaucuauua ccgagagccg caauauuugu 1620
uaccuuaaca uauucgaauu uuuaacuuuu uacuaauuuu guuuacgugu guuggggggg 1680
ggggaugguu aaauuaaaua aacacaaaua aacauauuca guacgauuca aagauuauuc 1740
aaaaaaauuu aaaaccaaau auugaaaaau aagccaacgg uggcaaauuu uuacaggcag 1800
cucuaagaaa aaauggauuu ugcaguagau caaugcauau gaaacaaaaa auucaaaaau 1860
auguuauuag cuuaugaguu ucucgaggua acgcuguuga guuuuuuagu uuuaacgauu 1920
uuugaguuuu ugauaucacu caaaauacca gucaaaauaa ggaaauuuuu guaaaaagug 1980
ugaaaaucaa cauauuuauu auuguuaacc aaaacauauc ucgauagagu uacuucacga 2040
agugaccaaa gaaaaucuau gaaaaauuuu agguggagcu gucaaguagg uaguuuuuga 2100
guuacaaugu ccaccgccuu ugaaaaaagc aguuuugaga aaaacgcauu ugguuugaca 2160
acuuuuauuu cccuuuguuu uuugucuguc aaaucguaaa gugauguacg ccggaauaug 2220
uuuuugaauc guagaguaau uuauaaaaga gacgagaaca gcuguuguac guuucucccu 2280
acgcucaggc uacugcaaag cgagucgaag guagggauau uaaacaugcu uuacuuccgg 2340
uaauuuuacu ccagucaagc ugaaaauuuu agagaguagu cuugaaguuu auuuauuuau 2400
uuauuuaugu acuuucauuu aaaauauaaa aaaaaaucaa acauuuaaaa caaaaaaaau 2460
uuucaaaccg uaacccuccc cucuaaacag cauucgcauu uaauuaagca ugguauuucc 2520
cucaaauaua uguguaucua uauauuuauu uauuuuuauu uuaucucuga accuauuucu 2580
uuuuccuuaa uauuguucug aagcuauuug cuuguaacau cuuaugcaau uuacuguuuu 2640
guuugagaau gggccacagu uugaguuuga aauuaaauuu auuu 2684
<210> 191
<211> 458
<212> RNA
<213> 玉米根萤叶甲属
<400> 191
gcuugaucuu caagguagaa caacgcaagu agaaaucuaa aacaugucug gacguggcaa 60
gggaggcaaa guaaagggaa aagcaaaguc ccgaucaaau cgugcugguu uacaauuucc 120
uguaggucgu auucaucguu uauugagaaa aggaaauuau gccgaaagag uuggugcugg 180
agcuccugua uacuuggcag cuguuaugga auauuuagcu gcugaaguuu uggaauuggc 240
aggaaaugca gcuagagaua acaaaaagac ccguauaauu ccuagacauu uacaauuggc 300
cauaagaaau gacgaggaau ugaacaaauu acugucagga guuaccaucg cccaaggugg 360
aguauugccu aauauacaag caguacuguu accuaaaaaa acugaaaaga aagcuuaaga 420
guuaguauuc cuuuuuaucc aacccggccc uuuucagg 458
<210> 192
<211> 2478
<212> RNA
<213> 玉米根萤叶甲属
<400> 192
acuagguaaa uauaccuugu auauuugucu auuaacggua uuauuuauau uauuuuaaag 60
ugcgcaugcg uguugugaca guuuauccaa uuuucuuaca auuucaggua cccucgaacg 120
caacuaaugu ucuuuuagau uguuagccag auuucacaca auacgugguc acgucaggcc 180
gccguauuca guuuuugauu ucaaacaaac ugacugcugu cugcauaaaa auccaaaaaa 240
ucuuuugcca auauuguugu gacauuuuau uacguauacu cguaaaggcg uaauaaucaa 300
cuugaaauug caauaaaacu aaaagcuaaa uaacgauuuu ucuaguaaua ucugucauac 360
gaauuugaau acuggaaaua gaugaaauua guaucguauu uuuucuuaaa uuuugucacc 420
caugaauuaa aagcuucaga gucaaauuag auacauuaau uuguaaugca gaaauuuaac 480
uguaacuucc ugucauggaa cuugauuuug auauuugaug guacuuucuu ucuauuguua 540
cugauaaaag auuaccaauu aaaaaauaua auguucauug uauucacaug auguauuaca 600
uuuguuggua cucuuuugcg acaauuucga ccaauguaca uuuagauuuu gagcuuuaca 660
gcaauggaac ugaaauuaac guucaagaua ucaauacaaa uaaaaagcuc aaaaucucug 720
gacgauaaga acacuuucau cacaugccaa ugcuugcuua acuuuuuuaa auuaaauuuu 780
uuuguuaaaa ucaaugcagu aaaaguuuuu uauguaagau aucucucccu aguguggaga 840
uaaacuaagg cauauagaau augacaguag uuauauucua uacaaaaaaa auuaaguauc 900
cauguggugg ggguaaaugu augaggguca acaggaucga uacugucugu guauuaaaua 960
aucuaugcuc uuagcgaaug uucccagguu aguguuaugu aaucgugggc cucaucggcc 1020
ucaacacuuu uuuuugucua cagugaagua agaaagcggu uuccaaagug caugaaguug 1080
gaugguguag aucugccccc accaauuagc uucgacauug cggaagagca accguuacca 1140
ccuugccaac agacguucuu auguaauggu gauggaggau ccauagugcg acaguuucuc 1200
gagcuguauu ucguaauaua ugauucagau aauaggcagu cccuucuuca ggcauaucac 1260
gaaaaagcca cauuuucaau gacaauggcc uacccguacg gcuauuccaa agacaguaaa 1320
ggaguaucgu gguugaauug guaugccacc gauaauagaa auuuauuacg aguucaagau 1380
ccagacagaa gaaacaaguu guuaagacag ggacaaguug cuguaguuuc guucuugcaa 1440
gauaugccgc acacgaagca cgauauucac aguuuuacag uagauuugac aguuuuuaca 1500
ccccagaugu uauguuugac aguggcuggu auguuuaaag aauugaaaag uggccacaaa 1560
guaccuccuu uaagauauuu cuucagaacc cuuguaauug uaccugcugg aucagguuuu 1620
ugcauagcaa augaagaacu ucacauaucc aaugcaacuc cggaccaagc aaaagaugcu 1680
uucaagacca ccguuaaugu agcuccggca ccagccccug ugauuaccuc uccuggaccc 1740
aguauaccac aacccgcugu gccagaugau gcuacaaaac aagaaauggu aaaacagaug 1800
uccgcaguau ccggaaugaa ucucgagugg ucgcuacagu gucucgaaga aacacaaugg 1860
gacuaccaga aagccauaau gguauuccaa aauuuaaacg cacaaggugu uguaccacaa 1920
gcagcauuua uuaaaugaua cgaagauuau guuaacuuug guuaauuaau ugacaguuag 1980
uuauaucuug gcaaauguaa auaguaucuu aaauuauagc uaauuuuuag uuuuauuauu 2040
guuuaaggua auguuaguuu aagaugucga auuuuaaguu uguuacauac gaaaaucaaa 2100
ucgaaaaaau guuaauaguc ccuuagaaau accgguagcc cuuaucuuag aagaaaggug 2160
guagauuuua aauaaaaacu ggguuacuuc acaaaaaaau gucuauuuua auuaguugau 2220
cucgauaacu uaauaugauu auauaaauac uggccuaacc uaacaaaucg gacugaaacu 2280
uuuaauauua ccuaaacaag caagauccau uuagacuaaa guuugaaguu uuggaauugu 2340
auugaaauuu gaacauuaca uaaacgguua auuuuauaug agaacuucuu agacaauaau 2400
acuaauuaac uauuuucaau gggaaauaag ccacaauuuu accaaaaaaa ugauuuuauu 2460
aacguuucga cgcccaag 2478
<210> 193
<211> 3274
<212> RNA
<213> 玉米根萤叶甲属
<400> 193
caaaacguca acaaucugac acgucuguaa uguuuagccu gacuuuugag uaaaauuacc 60
gaaaaacaua auuaaaauug auuuauuaag aguacauaau aacguacgaa aaugacugcg 120
guagaacaac cuuguuacac acuaauaaac uugccaacag auucggagcc cuacaaugaa 180
augcaacuaa aaauggauuu agaaaagggu gagguuaaag uaaaaauaag agcauuagaa 240
aaaauaauuc acaugauucu ggcaggagaa agguugccga auggauuucu aaugaccauc 300
auaagaaacg uuuuaccuuu acaagaucau uuggcaaaaa aacuauuauu gauuuucugg 360
gaaauaguuc caaaaacaaa uccagaaggu aaacuacuac aagagaugau uuugguaugu 420
gaugccuaua gaaaagaucu gcaacaccca aaugaauuuu ugagagguuc uacacuucgc 480
uucuugugca aacugaagga accagaauug uuggaaccau uaaugcccag uauuagagcu 540
uguuuggauc auaggcacag cuaugugagg aggaaugcug uacuggcaau uuuuaccauu 600
uacaaaaauu uugaagcccu cauuccagau gcuccugaac ugaucuccaa uuauuuggau 660
ggugagcaag acaugucuug uaaaagaaau gcguuuuuaa ugcuucuuca ugcugaccaa 720
gaaagggcgu ugucguauuu ggcaucaugu uuagaucaag uaaauucauu uggagauauu 780
cuacaacugg ucaucguuga guugauauau aagguguguc auuccaaucc ugcggaaaga 840
ucuagauuua uuagauguau auauaacuug uugaacucaa gcaguccugc ugucagguac 900
gaagcugcag gaacuuuagu cacccucucc agugccccga cugccguuaa agcugcugcu 960
agcuguuaca uugaguuaau uaucaaagaa agugacaaca auguaaaacu caucguuuug 1020
gacaggcuga uagcacuuaa ggagcuuccu aaucacgaaa gaauucugca ggauuuaguu 1080
auggacauac ugagaguacu cucugcuccu gacuuagaag uccgcaagaa gacuuuaagu 1140
cuagcccuug aauuagucuc uucacggaac auagaagaaa ugguauuagu auuaacaaag 1200
gaagugagua aaacgguaga cagugaacau gaggauacag gaaaguacag gcaauuguua 1260
guaaggacuc uacauucgug uuccauuaag uucccagaua ucgcacguag uguuauacca 1320
gucuugauug aauuuuuauc cgauaauaau gaacuggcug ccacagaugu auugcuguuc 1380
uuaagggaag ccauacagaa guuuaaagaa uugcaaccgu uaauuauuga gaaacucauc 1440
gaaacuuuca aagacauuaa auuggucaaa guccauagag cagcaauuug gauuuuggga 1500
gaauacgcga guacugcuuc cgauauagaa guuaucguug gagaaauuaa cagauuguug 1560
ggugaaggau cccucguuga agcugagcag aaguuaauag caggagauac ggaagagaau 1620
gcuccugcac cugcugcagg cgccaccacu uuaguuacuu ccgauggaac auaugcuacc 1680
caaucagcuu ucaacacugu cagccaaacc acuaaagaag cacgaccucc ucuaagacaa 1740
uaccucaugg auggugauuu uuucaucgga gccucuuugg caucuacauu aaccaaacug 1800
ucuuugcggu augaggaccu caccucuccu gcugcuagca auggauucaa ugccaaaauu 1860
augcuuauua uggcuggaau ucuucacuug ggaaaaucag gacuucccac aaaaucaaua 1920
accaacgacg auaaagacca cauucuguuc uguuuacgag uccuaucuga ucguucucca 1980
aucauuguug aaauuuucaa aaaauugugc cgcucggcac uaaaugagau gcuucuagcu 2040
aaggaaucgg uagaagcgau cucgcaaaag agcaaagaaa aaaacaagcg uacgauucaa 2100
acugacgacg cuauaagcuu ccugcaauua gagacagaua aaaguggaga gcuaggagaa 2160
aacguauucg agaugucgcu gucacaagcu uuaguaggag gucgaacggg agguggcgaa 2220
ucaguauuaa guuccaauaa auuagauaaa aucacacaac ugacugguuu uuccgaucca 2280
guuuauuccg aagcauacgu ucacgugaau caguacgaua ucgugcuuga ugucuuaauc 2340
guaaaccaaa cuaacgauac uuuacaaaac ugcacgcuag agcuggcuac uuuaggcgau 2400
uugaaguugg uagagaagcc acaaccuguc guauuggcgc ccaaagacuu uugcaacauu 2460
aaagcuaacg ugaaaguggc cucaacugaa aacggaauua uauuuggcaa cauuguguau 2520
gaugucauag gagcgggguc agauaggaau guuguaguuu ugaaugauau acacauagau 2580
auaauggacu auauagugcc ugcuaguugu acagauagcg aguuuaugag aaugugggcg 2640
gaauuugaau gggaaaauaa gguaaccguu aacacacccc ucacggaacu uucagaauac 2700
cucgaacauc uacucaaaag cacaaauuug aaauguuuaa caucagaaaa agcucugagc 2760
gggcagugug guuuuauggc agccaauuua uaugcaaaau ccauuuuugg agaagacgcu 2820
uuggccaacu uaaguauaga gaaaccuuuu aauaaacccg augcgccagu aagcggucau 2880
auuagaauaa gggccaaaag ucagggcaug gccuuaaguu uaggagacaa agucaauaug 2940
acacagaaga gcacacaaca uaaaguagua gcugcauaaa uaaaaacguu uuuuucuguu 3000
ucaacccuuu ucuucauuuu aaaucauauu cuguaauuua auuaauuuaa uaucauauua 3060
cugguuacac auuguuagau uauaaauauc uuuuaaagaa uaaaauuuau uuagcuuuua 3120
caaagagcgg aaagcaaucu uuaucuucuu cuaauaagau caacaucaag uuuucuggaa 3180
aauauaauau auaauuuaaa gauguuuagc auuuauuagu gaaauacaug gcuuacuauu 3240
uugaugacaa agucagugua auauagaaaa gcag 3274
<210> 194
<211> 3652
<212> RNA
<213> 玉米根萤叶甲属
<400> 194
gguagaugau gaugacccua auaaugaaaa caacucaagu cuucuuuuua aaaacugcaa 60
aaaagaaccc uacggagucc accucugaau uagguccaaa ucuuugaaca aaacuccuug 120
cuucgucuga aagagaaguu ccgagccauc cagguucucc uaaaguuaaa ucugguugga 180
cuaauuccaa gaaauuaaaa cuuuuuaaag cccaagcuac aaugccuucg uucucagaua 240
ggguuauagu acauguacug uagaccauga uuccaccugg cuucaauaau uuugcugcau 300
uuucaaacag uuuucuuugu aauggaacga augacuugau cucguuuucu gacgaccuau 360
uggccaguug uggucuuuuc ccaagaacac uacacggugc aucuaaaagu auuuugucga 420
acgauugggg ugcgaauggu ggaccgucua uaacauuuuu ggaacuuaaa guaucugaaa 480
uuauugcagu agaaucagcu ugaaaacuau acacuuuggc gccgaaaucc ucacagcguu 540
uuugaaguug ggcaaccuuc uuuggaguuu ugucuauggc uauuaauauu cccuuauucu 600
gcauuagcuc ugcuauauga guaguuuuau ucccaggaga agcacacaua ucaaguacua 660
caucaccagg uuugggauua agauuaugaa cacauacaau agaugguaua uuuuguagua 720
aaauaucucc gacugguaau aaacuuucgc uuaucgguac acauccugaa auuguuucug 780
uuacuucuac agcaauacca cugggcgcaa uauuaucagu ugcaaacaac uggugccucu 840
gcaucuuaac uaucccauuu ccaaugaaua uuuugaaguc gucuucauau auuuucugaa 900
gucccuuuuu acacuuuuuu gcaacaucgg cguaaauacu aacguuuucg uuuaucugac 960
aaccugaaac cauuccuaau acuccugggg caaauauaug agcaccccga agaauugcug 1020
cagcacaauc agugucuacu auaauuucuu uaucaaacuu uuuaaagccu gcuggacaau 1080
uuaaacuguc aauuauuauc acauugguaa aagagggaug uauguaaaca uuugguguau 1140
cauuacaauu uucugcaaaa uagguuuuua auaccuuuaa aacuuuggca guauuuguuu 1200
uuauuguauu uacucuuauc gaaguuauuu uuggugcaga acauaaccau ugcuguauuu 1260
uuaauaauuc uucauuugaa accgauguuu uuguuuuggu gacacuaucg ucaagaaaua 1320
aauucguuau uaauuuauug uugaacguug ucuuaaacgg ggaauuaggg uaaaccaucu 1380
uaaccuuuaa gauaauuuaa aaucaacgua aaauaauucu guaaacaaaa uuaaagucac 1440
aaaacgugca uacuugauag guuagaaguu gaugacuaaa aacuaaaagg ucacguguca 1500
ugcgccaacc aaugcgacac gcugguuccc aagacguggc augagcuacg auucucuuuc 1560
aaguccgcca uauugacauu cgacaacuuu uuugggagga caggugaaug uuauagcguu 1620
uuucaaagug uaagguguuu auuuucaaaa aguuuauaaa auaagcaauc acuaugggua 1680
auguguuugc aaauuuauuc aaaggccucu uuggcaaaaa ggaaaugagg auauugaugg 1740
uaggacucga ugcagcuggu aaaaccacaa uuuuauauaa acuuaaauua ggagaaauug 1800
uaacaacuau uccaacaauu ggauuuaaug uggagacugu agaauauaag aacauuaguu 1860
uuacaguaug ggauguaggu ggucaagaua aaauuaggcc auuguggaga cacuauuucc 1920
aaaacacaca aggccuaauu uucguaguag acaguaacga cagggaacgu aucacugagg 1980
cuaaagauga auuaaugcgu auguuggccg aagaugaacu uagagaugcc guacuucuca 2040
uuuucgccaa caaacaagau uugcccaaug caaugaacgc ugcagaaauc accgacaaac 2100
ucggucucca uucacuacgc aaccgcaacu gguacauuca agcuaccugu gcaacuagcg 2160
gagauggucu cuaugaaggu cuggacuggu uguccaauca auuaaagaac gccaaucgcu 2220
agaacauagg aaaagagagg agugcgcaaa aaagugugcg agugcagaug guuuuuuucu 2280
uguacucuug ucgugucuac uaccucgcgc gcgcgcgaaa cguuucguau accauauguu 2340
gcaauuuuug uguccgaugg ggaaauucgu cauguuucac uuuggggguu auaaaauugg 2400
cuguuggcga caaguggugu augaggugaa uccgaaaaua guuuuaggca auauaucaua 2460
ugaaucucag cuuucuuuag guugagaguu guuuuuguag uaaaagaacc uuaaacaucc 2520
uucauuggcg auuuauuauu uuuuaacugc augaaaaucu uaguuuuaau guuuuuauau 2580
aacacuuuua gauuguacua acuuuuaaca uuccacaaau uucucauaaa auugugauau 2640
cuuuucuaau accucuuaua ucaaugaacu uauuuuugca ggccaaaaua uuuucuuaga 2700
aaguaauuuu guaucaucua uaaaaauuua auuuugggaa uucaauuaaa gacuuuaacu 2760
ggcaaauuuu aaaauguuac aagguucuag ucacguauuu agucuggacu ugcacuuuau 2820
agcacuuguc gcagaaagca ucuaaccccc aaacacguau ggugaugucc uauuugcaga 2880
aaaugcacua uaauauacga uaguaauuau uuauuguccu cuuucuggug aaaacaggcu 2940
uuuuguaaca uauauuuuuu auuguuugua auagguuaaa auguacagua gacacuuaua 3000
uuuaaaauaa auauuauuuu uuaucuauca auccuuuuug uaacauaugg uaaggcuguu 3060
cguauuuaaa aaauaaaaca agaacauguu uuugaaaagu uuugggcacg gcucaaguau 3120
uuuauuguuu aaauuuaaga ugaaguacga augggcgcgc ccgaauccga caagauauug 3180
uauagccccg uauucguugu caaaccaacg ggucuugcuc ugguaguuaa uuucggcguu 3240
agaguucaag aaggaaguag auuuuuuuau acuuauagua aauauauuau gggauauuua 3300
aagacuggcc gauuggcuua ccagacuuac gucccgcaga ucuuaaccgg cgaugaguau 3360
ucgagguuug uuucugguca gauucgggca cgucguacug uacauaaaau acuuuuguuu 3420
acuucucaaa uuuguaacuc aguuguggau acuuugaggc guaauuuuau uuugacaaac 3480
uguggauuua agguuacauu auaagugauu uauuuaaaga aauauguuuu ugauuuaucg 3540
uggacauucc cuguuuguca gcuguauuau caaauaaauu uauacuauua aaaaaaaaaa 3600
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aa 3652
<210> 195
<211> 7631
<212> RNA
<213> 玉米根萤叶甲属
<400> 195
cuucacuuuu acggguaaac gugcggaagg uacguuccaa guucgaaccu agcgcuugau 60
guucugugua cacucuguac aauuaaaaug gcuuccaagu ucuuugugag uuaacuuugu 120
uaggugcauu uuaguaaauu ucugaauugg guuguuaaau uuagaguguu agugcauagu 180
aacuauuaaa uaguggccuu aaaaagugcu cuuaaugagg auuauaauaa gaaaaacgaa 240
cuuuuaacaa gaacaacgag uguccauucu aauguuauua caaguagcua cuaaaaauau 300
ugcaaaaaaa acuuucggua acuaaaaugg gacgguugca cuguuuauuu uguauuuucc 360
uauguuuuac cgucaucaac acgcagacaa cgaauauaca uggauucucg gaaaauuccg 420
uggauacauu ucuaucaccu caugggaaaa gugcaaaauu cgugcaccaa aaucacaaac 480
ccaaaauuga aaauugucag aacuacaaac ccucggugaa agaagaacag ccaggcggaa 540
cguacguaac aacgguuacc gcuaucgaug acgauccuag ggagggagga ggaacaauua 600
guuacaaacu aauucauaga gaaggagaac auguuuuauu ugacauagac aacguuacug 660
guguuuugac aacuauccag ccauuugauc gggaugaacc aguaaggcag aaggaacuuu 720
auguaaccgu acaagcuuca gacaacggca ggccaccauu agcagauguc uguacauuua 780
caguuaccau uaccgacauu aaugauaaug cgccacagcu ugauaaacug aaauacgaug 840
cacaaguuuc ugaagauuua aaaguaggaa gugaagugau gagaguuuuu gcuuacgaca 900
uugaugaugg ggaaaauuca agauuaucgu auaacuuuuc aaacgaaaau gcucaauuca 960
cccaguauuu caggauagau cgagauacug gcguugugua uuuaaaggaa gcuuuaacag 1020
acaaaaagaa uacuagauuu aacagugcug uuuauguagc cgauaauggc guuaacgauc 1080
aagaaggcca aaaagauuca accgcuaaga uaucuauaac aguaguaggg ucugauaaac 1140
agccucccag auuuacucaa aaaaugccug auggaaucuu ggagaucccc gaagauuuua 1200
aagacuuuuc uaaacauauu gucacagucg aagcaacguc caacauugcg gauccacaac 1260
uugcuuuuga auuggugaag ggaaagacau aucaaaccaa uaaagaccaa acguuucuuu 1320
uggaggcaga aggaaauaaa gcgcacauaa agcuagugcg uccacuggau uaugaaacag 1380
uaacggaaua uacucuaacu auucgaguaa aaaacaaaga uuuaauggau ucuuccauaa 1440
auauaccaau uaaaguauua gauguuaaug augaaauucc uaauuuccuu gaauuucuua 1500
aagguagugu cguggaaaau gacaagccag gugcacaagc gauucaagua agagcaaucg 1560
auaaagacgg aacugcugcu aacaacauug ugagcuauga acucguugac aauacagauu 1620
uguuugcaau aaaccgaucu acgggaguaa uuacgucgag aguggaguuu gaucgugaaa 1680
cuguaccucu auaucacgua aacguuaaag cuuaugauaa cucuccgucu gcuuuguaua 1740
acacgacauu gccuaacauu guaauucaga cauuccaaau caguauagaa gaucaaaaug 1800
acaacaaacc uguauuuacu cauccaauuu aucaguucag uaauauuacu gagcuugcug 1860
auaaaucgag uauuguuggu gaagucaaag cuuuagauaa ugacacggcu ucaguuauaa 1920
guuauaguau uacaaaugga aauauugacg augcguuuau gauugaaaau ucuaccggca 1980
gaauaagagu uaauggaaaa cuggauuacg agaaaaucga acaauacaac uuaaccguuc 2040
gcgcauuuga uggggcauuu gaagauuuug caauuguuuu aauuuccaua cuuaaugaaa 2100
augacgaacc uccaguuuuu gacgacuaua ucagagaaau ucaaauuaaa gaggaagaac 2160
cuaugauauc cggaugcguu guuagaguga cugcucauga uccagauauu aaagacaggc 2220
augcugauca acacauagua uaugaggucg cgaaagaaca gaaagauuuu uugaccguau 2280
cugccgaugg augcguacaa guaacaaaac cucucgaccg agauccgccu uucgguagcc 2340
caacacgaca agucuucauc uaugcucgug auaaugaugg aggcacaaau ucauuguugg 2400
ccacugcaga aauugaaauu auuuuaauag auauaaacga uaaugcuccc uuuuuaaaug 2460
uuacagaaau uguuuauuau gaaaaccagg auccagguuu uauagguaac cuaagugccg 2520
augauuacga ugguccugau aauggaccuc cguuugcuuu ucgauuauca gacacugcuu 2580
cagauaguau uagaucgaaa uuuuccauua ucggaaacca gcuuuucgcu uuagaaaugu 2640
uugauagaga agagcaaaaa uauuaugaca uugccauuga cauuacagau aguggaguac 2700
cuccacuaac aggaacuagu auucuuagag uuauaaucgg agauguaaau gauaauccag 2760
cuacagacgg aaacagcacg aucuuugugu auaaguacgu caaugggcca gaaaauuuca 2820
uggaaaucgg acguguauau guuacagacc uagacgauug ggauuuaaau gacaaagucu 2880
uuguucaaga agauaacuuu gaugaauuug uguuaaacca gcauaacaac gguaugauuc 2940
ugaugaaacc aacaacggcu gagggaacuu augagguuca uuacaggguc acugaaaccc 3000
augaacccac aauacacgaa cauacaguua augcaauagu cacgauuaca guuaaaguac 3060
uuccagagga agcgguugua aaaucaggau caauucgauu gagaggaaca acuaaggaag 3120
aauucauaga aaauucauug aauggaaaga gcaaaagaga cauauuacac caagaacucu 3180
ccaaaauauu aaauacaucu uuagcgaaug uugauguauu uacuguuuua aauucacccc 3240
accagaauag uucguuugug gauguucgau uuucugcuca uggaucucca uauuaugcuc 3300
cagagaaacu cgaaaacaaa guuacagauc aucaaaugga gcuugaacaa aaauuagaug 3360
uggaauucua caugaucaac guaaacgagu gccuuaacga aacaacgugu ggagcugaaa 3420
acucauguac gaacaaauua aacauaacac gagaaccagc uguaguguuu acuaacagaa 3480
cauccuuugu cgguguaaau gcauuuauug auccugugug ugccgcuuua ccaagagaug 3540
uuauggaaug uuucaacgga ggcguccuua ucgaaaacac agcguguaau uguccugcag 3600
gauuugaagg accacauugu gaaauccuag cuauaggauu uacaggaacu gguugggcua 3660
uguauccauc cuuugacgcu acaaacagga cugagauuau acugcauauu uuaucacaaa 3720
cugauaaugg uuugauauuu uacaauggac cuuuaaauau aagacaaacu ucuuugucua 3780
aagauuauau aucauuagaa cuuaaagacg gauauccauu acuucaaauu ugcaccggcu 3840
caagcacuca agaaauuuau cugaaagagc gcauucacaa auugagcgau ggaucguuac 3900
acaaaauaaa aauaggaucu ggauuugacg auauaucccu ggaaguagac gacuguggaa 3960
caacguguuc aauuuggacu aauaaacuac auaaaggugu uauccgagca aauggccccc 4020
uucaacuggg agguaugaaa aacagauuca ccgaucaaga auucaaacga auuugggacc 4080
auuugccacc gacugccacc cguuucucug guuguauuag aaauuugacg uauaaugaau 4140
uuuacuacaa ccucggugca ccuucugaug cauuccaagc guaucccgac uguaacuaug 4200
cagugaugca agcugugacu uucgguaucg acuccaauuu cuugguugcu auucugguuu 4260
guguagcaau uuugauaauu cuucuucugg caguaguugu acauagacgu aaacacgaca 4320
acuuuaacga aaaagaaauc gaugauacuc gcgaaaacau uaucaacuac gaagaugaag 4380
guggcggcga augugacacc aacuacgacc ugucuguuuu ccaucagaac aacauugugg 4440
acgaaaaacc auugaugaga gacaaccccg auguaccugc agauauaagu ggcuuuuuag 4500
auaacaagaa agacaacugu gauaaagacc ccgauaauuu gccuuaugac gacguucgcc 4560
auuaugccua cgagggagac ggaaauagca ccggauccuu aucuucucuc gcuucaugua 4620
cggacgaagg agauuuaaag uucaacuacu uaucaaguuu uggacccaga uucagaaagu 4680
uagccgacau guauggagaa gauccaagcg augaagacuc acacgaugga aacgaagaau 4740
ccuggugcua gacuaaauuc caugacuccu uagaaaguga cauuuuugua cuuaaauucu 4800
uuuauguuaa ccaaagauug cgaaauuuuu uggaauggaa cugaacaaac uuguacauau 4860
uuuugaaaaa ggauguuuca acuguuugca auacaagaau uguuacguau gcguuucaaa 4920
acauauauuu acauguaacc guucuuguau uguaaauauu uuuauacuau cuuuauacug 4980
acacuuagau aauguuaucu auuaaguggc aagcaguuug guuaagaaua acuuugaguc 5040
uucuauccca agguacugug auuugaacaa aguuagaaua agcaacuucc auaccaucaa 5100
uccacuucau uuuauucaac auacugguuu uccgaaacua aagaagcagc cuaauacaua 5160
gaguucaaua acuuuuauua aacaauccgu auuaggugca cauauucauc gcgcacaagu 5220
acauauugug gaacuguaca cugacgagga acaaauauuc aauaagaauc uuuaaagcga 5280
aaaauauuga aauacacgau cagauuuaac auauuauugu cuagcucaaa agaaaauaau 5340
uuuuaucuug acaauagaca uuuuucauau auaagcuagu agauuuauau caaaaucuau 5400
aaaaaaagug gcuuaaaagu uucuuugauu uuugcgauuc ucuugaauga cuucaguauu 5460
uuuuacaaaa uuauaagacu guuaaucguu uaagcuggca gcaugguaag auacguaugu 5520
gaaaacuugu auauuuuaca aaauaacuga aaauuaguau guugcgaaaa acaaauggaa 5580
acaaauauac agugaauguc gaauaguauu gucaaaauua aagucauaau uuuuaaagau 5640
uguccaaacu agguuguuga uguauguuaa ucucugaaug ccauccuuua gccaucauug 5700
uguauaucag cuguuuccuu uugcaguuug ugugguacuc cugaauguac ggaauaauca 5760
cauaguuuuc acgaucuuuu acuuuucuuu ucugcagcua auuacagggu gguuguuccc 5820
uuaucaauau ugaaauucuu auauaaucag uuuuuccaga cuuagaaaag uuaaaaaaac 5880
agcaucauau cccugcuaug uuuauucuuc guuguaagga cacucuaauu auuuccaauu 5940
agagaucuca agauguuaug uuuacuagca gguauugaac gaaaacaaua auaaaagcaa 6000
accgaguaau caacaauagu uacaauaccu acaauuuacu augccuucgu agcuuauauu 6060
ggagagauga guggaauuuc ucaauaggcg cauauacaca uaauaauaag acacauauuc 6120
aaauugguug gugguuaaau agcucaaaaa cgauacaaag gaaacauaua gguaacaaca 6180
aaauacaagg uaaaaauaga gguacuuaca aucaggacgc uccaacgaag ucacuauuuu 6240
acgucuuuac acucgaaucg ucugaaugca ucucuuuccu uauguccucg ggguagaauc 6300
uugcaccuca agugguugaa aguuccuuuc uucgucacau guggauuucg cuaauaugcg 6360
aacaaggaac aacacaggcg ucaaaaagga auaugauuuc gaacuauuua aaacaaucaa 6420
aucaauuuau cggaauucca acacaagauu uauaucagca uuucaguagu aaucucacca 6480
uauuccaaau uugucuuuuu guauccaucu uauuuugucu uuaucuuacu uuauauuguu 6540
uuguuuauuu uuuauuuauc uguauccuua ucuuuuucuc uuugacuauc acagcaaauu 6600
uuuguaauuu ggauuuacaa uuaguuauga cuuuaaugaa aacaauacuu accaucucuu 6660
uccuuaaauc gugcgauaua auuuaucaac aauguaguau cauaccacaa caaugucuaa 6720
uaugaaacau acgagaggcc uuaugcauau gccuuaacac guuuaggcac aaauaccaau 6780
aaaaaccuua cacgguucgg caauaaaaug agagaaccgc uauuuuauau acacgcauuu 6840
uuucuauuuu uauuaggauu uuguuaguuu uguuauuacu uaguauaaau uuaggauagu 6900
uauuuuguau uuuuauucuc acguagggcu cagucgacgu agucguuuga auaacauuuu 6960
auuuaaaacu gcagguuuga gaauagccau ucauuggugc cguauuuaaa aaaagauuuu 7020
aaauguuucu uuacuggaua uaucgaaaua caguuuuuuc uuuacguaag gcaguuagau 7080
uauuaaaaua gucuugugaa agcguauagc ucagauguuu uuacaauaua guuucuaaau 7140
uagucuuaaa aggcaacuau ucuuauugca auauuuccca agcuaaauuc acucgagacu 7200
uuggacucau aaauauacaa ugacugaguu cuacuugauu uccguuucau ugacuauuua 7260
auauuuauuu uuacaagucu ugaugaaaau uuggaaauac augauaucaa agcaauuuau 7320
aaucguaauu aggcuaauag uauccuaagg ugauaguauu uccugauuuu caucaauuau 7380
auauauauau uaguaucaca aaauacaguc uguagagacc cgugucauag auaaccuaac 7440
cuuccauugu uacacaucuu guuaacaagu cgauuugaag uggugccggu ugugaagaug 7500
cguaucuuga uguuccacgg cuaaaaucgg uucguucaca aaagcuuuua uauauuuuuu 7560
auuuacuuuu cuucuuaaau guccggcuug aauuuucaag auccagugcu gguacacagg 7620
agggcgcgac g 7631
<210> 196
<211> 1028
<212> RNA
<213> 玉米根萤叶甲属
<400> 196
gucagugcug ugugcuguca cugucacuac ucuaguguca cgauuuuuua gcccugaaau 60
ugucagauua ucacaugaca uuuugagaaa acuaaauuag uaagugaaca cgaauuuagu 120
ucaaaaugcc uuucuguggu cccaaauugu cccucugcgg ccugauuauc agugcauggg 180
guaucaucca guuggguuuc auggguguau ucuauuacau uggggcugug gcuuuagcag 240
aagauauucc agagguugag uuuaagggcg auuuagacaa auuuuauagc gacgucaaca 300
cggguuucac acagaaugcu uacaacugcu ggauugcugc ucuccuauac cugauaacau 360
uagcaguauc agcucaccaa uucugggcca acaacagauc aucauugaac gucuaagaau 420
cuuuuaauuu gcuuuuguau auauuuauuu acauuccuuc uccagcugua gauuuuaaau 480
guauagaccc ucaaauacuu cagaguacca auuuuucugu uaaacuuagc ggacucguac 540
accaaagcaa uauccaaacg caacaugaaa cacucaacau cccauaaaua ucagagguua 600
uguaucccuu gaacgcgcuu aaaacuaccu aggaguccuc gaacuuuguu caccgacgca 660
augcggauaa ggaucaucca ccgaaaacca gaugcgacac ucagugcaua uuaucuuucc 720
acaguguaug uuauaugcga uuucgauagg guauugcuuu gguguacgag uccgcuuagg 780
ucagucaaug uagcauguug uuguuuuuaa aguuuucauu gguacuaaaa cuuuuguuca 840
ccaacguagu auuauaaaaa ucauaguaau auaaauaacg cugcaauguu augaaucuua 900
ccaaauuauu uuaugguucu guuguauuag ugauuugaau uuaaaaauug uacacauuua 960
cuaugaaaua aauugauuua uuguuaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1020
aaaaaaaa 1028
<210> 197
<211> 3012
<212> RNA
<213> 玉米根萤叶甲属
<400> 197
auuuauggua cacaucacua aaguauuaau caccacacuu uauaaauuca aauuuuuaga 60
guuggaaucu acaaaguuag ugauaauuag aaccauugug gcauucagaa acggucuaaa 120
cuuucaaugu uagcaaccuu uuguauauag cuacaauacc gauauaacag ugaacugaaa 180
aacucuagug uagugauaca aagcauuuac caugggucuu accauaucag caguguuuaa 240
uagguuguuu aguaaaaagc cuaugagaau uuuaauggua ggauuagaug ccgcagguaa 300
aaccacaauc uuauacaaau ugaagcuugg ugaaaucgua acuacaauac caaccaucgg 360
cuucaaugua gaaaccguug aguacaagaa uauaucuuuc acgguauggg auguaggugg 420
ccagacgaga aucagaaaac ucuggagaca cuauuucgcc aacacugaug gacucauuuu 480
ugugguugau uccaacgacc gagaccguau cgcggaagcc gaagaagaau ugcacaauau 540
guuaggagag gacgauuuaa gagacugcau uuuguuaaua uucgccaaca aacaagauuu 600
accgaacucg auguccacug cugaauugac cgauaagcuu aaguugcaca cuuugaagaa 660
uaggaggugg uacauacaag ccacaugugc uacucaaggg aaugguuugu acgaaggacu 720
agauugguug ucgaaugaau uggccaagug aauagguaga uguugugaag aguagcuuuu 780
auauuuuuua uugauaaugu aaaauccugu uuuauuugcc ucaguuuggu agaauucaua 840
caauauugcu ucuauguuga uaacuuuaua uguuucuauc auuaaaauau ggauaauuuu 900
auccaaaauc uagcuaaacc acuauauaac gcguuggcug uguuacgaga caaauagacc 960
ucguauuaua cuaacaguug gcucugucga cuauguugua auacacugug gcauuacugg 1020
acauauaugc cucguaaagc acucaaugcg ggcuuauaaa uaucucuaaa ugcagccaaa 1080
guauuaaucu uagcuuuaac ucuugauauu uuucaggauu uguguauaau uuauugaaag 1140
uuuaguuaaa uauuacauuu uucuucgauu uuguucauuu aggcaaucuc acauuauuua 1200
uauguaacau guuauugaau cauacccgcc auagccaauu auauacccgc cauagucuaa 1260
uuucagagau uauuuuuuug uuuuauugac gugaguacau uuuuuaagaa auucguuuca 1320
acauuuuugu cucuccucua uucauucauu cauuaaagcc ggauuuacau auacaaguac 1380
uuggauccga gauaucagua gaacugcgaa uuuuuacauu aaauaccagu gauucaaagg 1440
aaauauucau uauaucugca auagcuucau augcauuauu uauuuuguug auaucacaau 1500
auuccuugag uuuacauucc acaaacauuc aaaugaucag uaaaaacuaa uaaacugucc 1560
caucuguucu ccagacaauc uuaguuccac uuuuuuuuuu auuuguaaau uacaugugcu 1620
cuuguauugc uacuugguuu guguucaacu uccauccaug cucguaucca aguaacaaga 1680
ggcccugaga aggcguuuau acuucugaca ugcacucuga guagaguaac ucgaauucaa 1740
guacuuguaa gggcgacuuu agaguuacaa ucccuaggga uuauaucuaa ugccauggug 1800
uucaaaaucc uaugcuuagg ugagguggau uauuccuuug ugucauguau aguuuccugu 1860
augucuuugc ugcuguugug acucuuuuuc auuucuuccu guccuugcac cgaaauuucg 1920
agucuuguuu ugaauuuaau aguauuuugu auuuugacaa cgaaacccga uuugggcuuc 1980
gaaacguuaa uaaauucauu uuuuaguaaa auuguggcuu auuucccaua aaaaauacgu 2040
aauuguuuug aauuauuugc ucaaucuuau caauuguuac auuuacauau uuuuuauuug 2100
ggucgauuuu cuuuguuuau ucacaaaauu guuauaaccu gucugagaaa ucuuucaaau 2160
guuuuuuuac auuuccauua uucaauauua cacaauaucu cuauugggaa cgugcuuagu 2220
uuaucucuau uaggaagacu aguuuuaaaa acuaaaaaug uuuucuucga uaguaaaaua 2280
aacaaccagu guuuaacauc uuugccacau uuaaagauau uuauaacacu acguugggug 2340
cgucaugagg cauauauguc ccgaauaccc ccauuugcca cuguguuacg acacaguuga 2400
cguagccaag cguuagugua guacgaggug ucccguaaca cagccaagau guuaacaaua 2460
aacauaaaca aaauacaauu uugugcuacu aucuaaauaa gaugucaaug aguuauagaa 2520
caaagaauau uguaugaauc uauucgacca uaucauaaac ugacuuuuuc auguaguaaa 2580
caacguuucu gcaacuccuu cuucugugaa uucacuuuuu guucuaccua gauuuaaaua 2640
uuuauauaaa uaaguuuccu auuauauacu acaguggaac cucgauaacu cggauuaauc 2700
gggaccgcgg ccgauccggg uuaucgaaaa uccggguuag ccggagaaua uaguaaaaau 2760
uaauaaauaa ccuccauuau aauuacaaaa acaugaaaca cauaugcaca guacacaucu 2820
aaauuacgua uaguuguaua gaguguagag uuuuguucau uucuugguaa aaaacucagu 2880
cauacuguag agauguaccg acaccauaau augguagguc uggauccugc guacaaaaaa 2940
aaauugauaa auagcaagcc gaaaauuugu uguuagcuug ggggugucua gucggacaga 3000
cauugauaua ug 3012
<210> 198
<211> 1212
<212> RNA
<213> 玉米根萤叶甲属
<400> 198
caacacugcu aucugggcug uugcacuaau cauauggguu gguuguuagu ucacuuuuuu 60
cuacaguacc augugucacu agcauaauac ucaaaaaauu aauauuuaau uuugugaacu 120
uaguuaacau cauuuugaaa aaugcgguac acuuugaguu acaucggugc uacccuagcg 180
uccacuguaa cacugauuuu ugcccucuac uacugccuca cgggaaaagg agagcaaguu 240
aguuuagcau gguuauuguu gaaugugucu ccccacaugu gggcaggucu aggaauuggc 300
cuugcuguau cauuaucagu uguaggagcu gcugcaggaa uucacacuac aggagucagu 360
aucguaggag cugguguuaa agcccccaga aucaaaacca aaaauuuaau uucuauuauu 420
uucugugaag cuguggcuau cuauggguua auuauggcua uaguacucug uggaaguugg 480
aagaauuucg auguagaccu auucaaccuc aaaacucaua acuuugcuca aaaccauuau 540
ggaucacaug uuauuuuugg auccgguuua acuguuggau uuguaaaucu auuaugugga 600
uuuuguguug gaguaguugg uucuggugca gccauuucug augcagccaa uucaucauua 660
uucgucaaaa uuuugauuau ugagauuuuu ggaagugcca uuggucucuu cggucugauu 720
guuggaguau acuugacguc aagaggcucu augguuuaaa uguucaguaa augaacauga 780
aaaauaaaau gaauaauuug uuaaaagugu gugugaugaa gauacaacua uuuucacuag 840
uuuacgcccc aaaauauuuc auugugguuu uugaacaaua uuuaaaaucc auucuaaauu 900
aaaauucuuc aaauaauugu ugaaaacaga uucaaggguc uuuaaucagu guauauaaua 960
guauuaaaua aauauuccac uaauuuuguu aaucauuguu aaacauugua uaguuacaaa 1020
agaaauuuuu aauuuagaau aauaugcuug uacagcaugu agucccacua guauuaguau 1080
uuaaaaacug uauuaaaaau ugucuuuucu augucuaaua aaauuacaga cgauuuuguu 1140
aaauuuuaau acagaucuaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1200
aaaaaaaaaa aa 1212
<210> 199
<211> 7646
<212> RNA
<213> 玉米根萤叶甲属
<400> 199
cggauuucgg agaguucgau ucguugucga gccuucaaaa uggcuaccaa cgauaguaaa 60
gcuccguuga ggacaguuaa aagagugcaa uuuggaauac uuaguccaga ugaaauuaga 120
cgaaugucag ucacagaagg gggcauccgc uucccagaaa ccauggaagc aggccgcccc 180
aaacuaugcg gucuuaugga ccccagacaa ggugucauag acagaagcuc aagaugccag 240
acaugugccg gaaauaugac agaauguccu ggacauuucg gacauaucga gcuggcaaaa 300
ccaguuuucc acguaggauu cguaacaaaa acaauaaaga ucuugagaug cguuugcuuc 360
uuuugcagua aauuauuagu caguccaaau aauccgaaaa uuaaagaagu uguaaugaaa 420
ucaaagggac agccacguaa aagauuagcu uucguuuaug aucuguguaa agguaaaaau 480
auuugugaag guggagauga aauggaugug gguaaagaaa gcgaagaucc caauaaaaaa 540
gcaggccaug gugguugugg ucgauaucaa ccaaauauca gacgugccgg uuuagauuua 600
acagcagaau ggaaacacgu caaugaagac acacaagaaa agaaaaucgc acuaucugcc 660
gaacgugucu gggaaauccu aaaacauauc acagaugaag aauguuucau ucuugguaug 720
gaucccaaau uugcuagacc agauuggaug auaguaacgg uacuuccugu uccuccccua 780
gcaguacgac cugcuguagu uaugcacgga ucugcaagga aucaggauga uaucacucac 840
aaauuggccg acauuaucaa ggcgaauaac gaauuacaga agaacgaguc ugcaggugca 900
gccgcucaua uaaucacaga aaauauuaag auguugcaau uucacgucgc cacuuuaguu 960
gacaacgaua ugccgggaau gccgagagca augcaaaaau cuggaaaacc ccuaaaagcu 1020
aucaaagcuc ggcugaaagg uaaagaagga aggauucgag guaaccuuau gggaaagcgu 1080
guggacuuuu cugcacguac ugucaucaca ccagauccca auuuacguau cgaccaagua 1140
ggagugccua gaaguauugc ucaaaacaug acguuuccag aaaucgucac accuuucaau 1200
uuugacaaaa uguuggaauu gguacagaga gguaauucuc aguauccagg agcuaaguau 1260
aucaucagag acaauggaga gaggauugau uuacguuucc acccaaaacc gucagauuua 1320
cauuugcagu gugguuauaa gguagaaaga cacaucagag acggcgaucu aguaaucuuc 1380
aaccgucaac caacccucca caagaugagu augaugggcc acagagucaa agucuuaccc 1440
uggucgacgu uccguaugaa ucucucgugc accucucccu acaacgccga uuuugacggc 1500
gacgaaauga accuccaugu gccccaaagu auggaaacuc gagcugaagu cgaaaaccuc 1560
cacaucacuc ccaggcaaau cauuacuccg caagcuaacc aacccgucau ggguauugua 1620
caagauacgu ugacagcugu uaggaagaug acaaaaaggg auguauucau cgagaaggaa 1680
caaaugauga auauauugau guucuugcca auuugggaug guaaaaugcc ccguccagcc 1740
auccucaaac ccaaaccguu guggacagga aaacagauau uuucccugau cauuccuggc 1800
aauguaaaua ugauacguac ccauucuacg cauccagacg acgaggacga cggucccuau 1860
aaauggauau cgccaggaga uacgaaaguu augguagaac auggagaauu ggucaugggu 1920
auauugugua agaaaagucu uggaacauca gcagguuccc ugcugcauau uuguauguug 1980
gaauuaggac acgaagugug ugguagauuu uaugguaaca uucaaacugu aaucaacaac 2040
ugguuguugu uagaagguca cagcaucggu auuggagaca ccauugccga uccucagacu 2100
uacacagaaa uucagagagc caucaggaaa gccaaagaag auguaauaga agucauccag 2160
aaagcucaca acauggaacu ggaaccgacu cccgguaaua cguugcguca gacuuucgaa 2220
aaucaaguaa acagaauucu aaacgacgcu cgugacaaaa cuggugguuc cgcuaagaaa 2280
ucuuugacug aauacaauaa ccuaaaggcu auggucguau cgggauccaa gggauccaac 2340
auuaauauuu cccagguuau ugcuugcgug ggucaacaga acguagaagg uaaacguauu 2400
ccauuuggcu ucagaaaacg cacguugccg cacuucauca aggacgauua cgguccugaa 2460
uccagagguu ucguagaaaa uucguaucuu gccggucuca cuccuucgga guucuauuuc 2520
cacgcuaugg gaggucguga aggucuuauc gauacugcug uaaaaacugc cgaaacuggu 2580
uacauccagc gucgucugau caaggcuaug gagaguguaa ugguacacua cgacgguacc 2640
guaagaaauu cuguaggaca acuuauccag uugagauacg gugaggacgg acucugugga 2700
gagaugguag aguuucaaua uuuagcaacg gucaaauuaa guaacaaggc guuugagaga 2760
aaauucagau uugauccgag uaaugaaagg uauuugagaa gaguuuucaa ugaagaaguu 2820
aucaagcaac ugauggguuc aggggaaguc auuuccgaac uugagagaga augggaacaa 2880
cuccagaaag acagagaagc cuuaagacaa aucuucccua gcggagaauc caaaguagua 2940
cuccccugua auuuacaacg uaugaucugg aauguacaaa aaauuuucca cauaaacaaa 3000
cgagccccga cagaccuguc cccguuaaga guuauccaag gcguucgaga auuacucagg 3060
aaaugcguca ucguagcugg cgaggaucgu cuguccaaac aagccaacga aaacgcaacg 3120
uuacucuucc agugucuagu cagaucgacc cucugcacca aaugcguuuc ugaagaauuc 3180
aggcucagca ccgaagccuu cgagugguug auaggagaaa ucgagacgag guuccaacaa 3240
gcccaagcca auccuggaga aauggugggc gcucuggccg cgcagucacu gggagaaccc 3300
gcuacucaga ugacacugaa cacuuuccau uuugcuggug uauccuccaa gaacguaacc 3360
cuggguguac cuagauuaaa ggaaauuauu aauauuucca agaaacccaa ggcuccaucu 3420
cuaaccgugu uuuuaacugg ugcggcugcu agagaugcgg aaaaagcgaa gaauguguua 3480
ugcagacuug aacacaccac ucuucguaaa guaaccgcca acaccgccau cuauuacgau 3540
ccugacccac aaaauaccgu cauuccugag gaucaggagu ucguuaacgu cuacuaugaa 3600
augcccgauu ucgauccuac ccguauaucg ccgugguugc uucguaucga acuggacaga 3660
aagagaauga cagauaagaa acuaacuaug gaacaaauug cugaaaagau caacgcuggg 3720
uucggggacg auuugaauug uauuuucaac gacgacaaug cugaaaaguu ggugcugcgu 3780
aucagaauca ugaacagcga cgauggaaaa uucggagaag gugcugauga ggacguagac 3840
aaaauggaug acgacauguu uuugagaugc aucgaagcga acaugcugag cgauaugacc 3900
uugcaaggua uagaagcgau uuccaaggua uacaugcacu ugccacagac ugacucgaaa 3960
aaaaggaucg ucaucacuga aacaggcgaa uuuaaggcca ucgcagaaug gcuauuggaa 4020
acugacggua ccagcaugau gaaaguacug ucagaaagag acgucgaucc ggucaggacg 4080
uuuucuaacg acauuuguga aauauuuucg guacuuggua ucgaggcugu gcguaagucu 4140
guagagaagg aaaugaacgc uguccuuucg uucuacgguc uguauguaaa cuaucgccau 4200
cuugccuugc uuugugacgu aaugacagcc aaaggucacu uaauggccau cacccgucac 4260
gguaucaaca gacaagacac uggagcucug augagguguu ccuucgagga aacuguagau 4320
guauugaugg acgcugccag ucaugcggag gucgacccaa ugagaggagu aucugaaaac 4380
auuauccucg gucaacuacc aagaaugggc acaggcugcu ucgaucuuuu gcuggacgcc 4440
gaaaaaugua aaaugggaau ugccauaccu caagcgcaca gcagcgaucu aauggcuuca 4500
ggaauguucu uuggauuagc cgcuacaccc agcaguauga guccaggugg ugcuaugacc 4560
ccauggaauc aagcagcuac accauacguu ggcaguaucu ggucuccaca gaauuuaaug 4620
ggcaguggaa ugacaccagg uggugccgcu uucuccccau cagcugcguc agaugcauca 4680
ggaaugucac cagcuuaugg cgguugguca ccaacaccac aaucuccugc aaugucgcca 4740
uauauggcuu cuccacaugg acaaucgccu uccuacaguc caucaagucc agcguuccaa 4800
ccuacuucac cauccaugac gccgaccucu ccuggauauu cucccaguuc uccugguuau 4860
ucaccuacca gucucaauua caguccaacg agucccaguu auucacccac uucucagagu 4920
uacuccccaa ccucaccuag uuacucaccg acuucuccaa auuauucacc uacuucccca 4980
agcuacaguc caacaucccc uaacuauuca ccaacaucuc ccaacuauuc acccacuuca 5040
ccuaguuauc cuucaacuuc gccagguuac agccccacuu cacgcagcua cucacccaca 5100
ucuccuaguu acucaggaac uucgcccucu uauucaccaa cuucgccaag uuacuccccu 5160
acuucuccua guuauucgcc gucgucuccu aauuacucuc ccacuucucc aaauuacagu 5220
cccacuucuc cuaauuacuc accguccucu ccuagguaca cgcccgguuc uccuaguuuu 5280
uccccaaguu cgaacaguua cucucccaca ucuccucaau auucuccaac aucuccaagu 5340
uauucgccuu cuucgcccaa auauucacca acuuccccca auuauucgcc aacaucucca 5400
ucauuuucug gaggaagucc acaauauuca cccacaucac cgaaauacuc uccaaccucg 5460
cccaauuaca cucugucgag uccgcagcac acuccaacag guagcagucg auauucaccg 5520
ucaacuucga guuauucucc uaauucgccc aauuauucac cgacgucucc acaauacucc 5580
auccacagua caaaauauuc cccugcaagu ccuacauuca cacccaccag uccuaguuuc 5640
ucucccgcuu cacccgcaua uucgccucaa ccuauguauu caccuucuuc uccuaauuau 5700
ucucccacua gucccaguca agacacugac uaaauauaau cauaagauug uagugguuag 5760
uuguauuuua uacauagauu uuaauucaga auuuaauauu auuuuuuacu auuuaccagg 5820
gacauuuuua aaguuguaaa aacacuuaca uuuguuccaa cggauuuuug cacaaacgua 5880
acgaaguuaa aucaaaacau uacaacugaa acauacgucg guauguacug ucaaugugau 5940
cauuaggaaa uggcuauuau cccggaggac guauuuuaua aaguuauuuu auugaagugu 6000
uugaucuuuu uucacuauug aggagauuua uggacucaac auuaaacagc uugaacauca 6060
uaccgacuac uacuaauaua aagauaaaua uagaacggua agaaauagau uaaaaaaaaa 6120
uacaauaagu uaaacaguaa ucuuaaaaau aaacaaauaa guuuccguuc cgacagaacu 6180
auagccagau ucuuguagua uaaugaaaau uuguagguua aaaauauuac uugucacauu 6240
agcuuaaaaa uaaauaauua ccggaaguaa ucaaauaaga gagcaacagu uagucguucu 6300
aacaauuagg uuugaaaaua aaaauuauaa ugaaauauac aaagacuaaa aguuuaaaua 6360
auaugaaaac cauuuuuaac ccuccguuag ucgcuaucgg ugucacacac cgacgacaga 6420
auuauucauu cgggauuuac aaaauaacug uuuuuuucua uaccacuuuc uguaccucuu 6480
ccuaucaacu aaccucgugu uaguauacau ucuuaccuac uuggguacag uugugcgagu 6540
uuuauagcua uuuucggugc aagacuccgu agcgacuaac ggugugguua uuacuuuauu 6600
ggcguaacuu gcaguucagg uaaagauuua gcaucuuauu auugcauuuu aucgguaagu 6660
uuuggucaaa uuuuauuuau agauguccuu ugaagccgaa cgaaaacguu uguuggaauu 6720
augggaaaug guuccuagcg aauauuuuua uauuacaaau aacaauaugu uuauuguuau 6780
acuaggguuu uucauuuuau aucugucguu uuucaaugaa gcauuuucaa aaauauuuuu 6840
uagucauucc uauacacaau auaaaauauu uguaugaauu uuauagcaau aacuuuuuuu 6900
uuuaaauugu cggucucuga caccguagcg acucuugaug cuccguuuau ccuagcgacu 6960
aacggggguu aaugugaauu uaucacaagc acaaagcaca uagauaaaac caaaccuaua 7020
aguuacuucu aauacaaaau aauuaaaugg uuuugaaguc auaaucucgc caguuuugug 7080
aagaauuauu aaggaccaga caggucuuca agucgugccg uuuucugucu gcuugucucu 7140
gcaauaaauc uuaaacgaaa aguccuaaug auuuaaaacu uaauauuaug uauauuugua 7200
cauaggauau uuacgaucca aggaagaaca aaggaauuug gugucaaugg gucaaaguuu 7260
accaaaaaaa guuacugaua auuccgauua gaaaggacgu agagccuuuu accaucaguu 7320
agagguuaga gguucggcaa aggcuuuugu uacuuuacau aauucuguca gguccuuugg 7380
uacucauuau acaaaagcuu uuaauuuugc accuuucacu uccuuccacu aauucgguac 7440
cacggacuau cgaaggacca aauauaauuu uuuaacauac cuaaagcccu ugccgaaugu 7500
cuaaccgaag auucaauauu ucacauccca ucuggaguuc ucuguaacuu ugagaugauu 7560
uuuaaucuau ugacgcgaaa uucaauaguu uucuuggacc aaaaggacgc uguguaccaa 7620
guuucaugac uuuuccugaa agaguu 7646
<210> 200
<211> 2600
<212> RNA
<213> 玉米根萤叶甲属
<400> 200
guugacauug acagugggga caccuauucu ggcaauugga auuaauuugu caauaucauu 60
uuuugucgug aaauugugug uuguaggaga aaguaauauu auuaaaaaau gucgucaaau 120
auucaaaagg cccagcaguu gauggcggau gcagaaaaga aaguaacauc ucgagguuuc 180
uucggaucuc uauuuggggg aucaagucgu auugaagaug caguggaaug uuacacaaga 240
gcugcaaacc uuuuuaaaau ggccaagagc ugggaugcug ccgguaaagc cuuuugugag 300
gcugcuaauu ugcauuccag aacuggugcu cgucaugacg cugccacuaa uuauauagau 360
gcugcaaauu guuacaaaaa agccgaugua uuugaggcug uaaacugcuu uauaaaagcu 420
auagacauuu auaccgaaau gggucgcuuu acaauggcug caaaacacca ucagacuauu 480
gcagaaaugu augagacuga ugcuguggac aucgaaaggg cuguucaaca cuaugaacag 540
gcggcugauu acuucagagg agaagaaagc aaugcuuccg ccaauaagug ucuucuuaaa 600
guggcucaau augcagccca acuugaaaac uaugaaaaag cagugggaau uuaucaagaa 660
guggcuuaug cagcucugga aagcucucuu uuaaaauaca gugcaaagga auacuuauuc 720
agagcugccc uuugucaccu uuguguugau guacucaaug cacaacaugc uauagaaagc 780
uauauuucaa gguaucccgc auuucaagau ucccgugaau acaaacuuuu gaaaacccuc 840
auagaaaaca ucgaagagca aaacguagau ggauauacag aagccgucaa agauuacgau 900
ucaauuucuc gucuugauca gugguauacu acaauucuuu uacguauuaa gaaacaagua 960
agcgaaagcc cugacuuacg uuaagacgua uuuagaaauu ucuuauuaau auuucuuuaa 1020
guguuauuua agagacccau aguuuauuua uuguuggauu auuauuugag uaugucggua 1080
uacaugaagu gauuuaaccu ucauauagca uuuuuaaaua uggaagcgau acauuuaaca 1140
uucauuuuuu acaaaaaaaa uuaguauagc auuuugcaau uuaugguuuc uaaggauaaa 1200
guuaucaaau gagcaaauuc acuuaaguuc uaacauguuc gauaguauaa uaagauuaac 1260
gauacuuuua acgauauauc uguauaaaua uucaaugaaa auacguaagu auuaagcaau 1320
uaccaacuaa uauuauuauu ugcucauuua gguucucauu uuucaauuuu ugaaauuguu 1380
auucuugcua uuagaguaaa aagacuuaac aauaacaguu gguacuacaa uugcuuacua 1440
uauuagcagg caaaucuugg uuaucacaac accaucuuau aucagacuuu agaaacagcu 1500
uguaaaauua uauauaauga gcacuguuau auugauucuu uuaaauauuu ucucaucauc 1560
uaaaggugcg aacugacuug agcauuuuuc uccgaacgaa cugaaugagc agagcgugcu 1620
guagcaaauu gcucuagucu cuagucagua cguguucacg agcaaaauuc ugcugaacug 1680
aauuuucgga ggagugucgg uaaucguuga aagaucaaaa gcuaucauuu guucuuuugu 1740
gcucaauagc uacagacauu uaguguauuu gguguguucc gauugauuua uaugagcccu 1800
uuucuguagu auguaaucca aucaucauaa ucauucaacu cggaucuauc cacugcugga 1860
uguaggucuc ccucagucug uuccauguau uucuguuuug ugcuguuugc auccaauuuu 1920
ugucgagccg uuuuauguca ucagaccauc uuguuggcgg augaccucua cuucgauauu 1980
cuuguuguca ugguauccag uguauuauuc gcugugucca uuuguuauuc gacauucuag 2040
cuaugugucc agcccaguuc cacuuuaggg ucauaauuau uucuauagca ucugucacuc 2100
cuguucuucg ucuuauuucc uuguuuguaa uucgaucccu acgagaaaug uguaauauug 2160
agcguuccau ggcucuuugg guaacacaaa uuuuauuucu uacuuuguug guuauuguua 2220
aaguuucugc accauaugua aguacuggca acacacacug aucaaagacu uuccuuuuga 2280
gacauacaua uguguaaucc aauacacggg caauauuauc auaaaauaua gaaaaugaug 2340
cauggauauu aauagcugaa guaggaugua ccuccaaaga agcaaagaaa aagucauauu 2400
uauaugaauc uccugucgcu auuaaccaca aaguaacagu uaagcgaucu cgaagagaua 2460
uauauuauac cuuuucgaaa uguuuugcaa uuguaggacc uacuauaguc cgucccaucu 2520
ugacuuuaca guauggggag caggggcggu gcaguccuua ugagaguuuu uagcgcggug 2580
augccgauuu uaccaaaaac 2600
<210> 201
<211> 4648
<212> RNA
<213> 玉米根萤叶甲属
<400> 201
uuggcaauac gucuaauacg ucagucaaau gaaucaaguu guugggauug ggauuuaaca 60
aauacgucgu ucuaaauuuc uaaaacugca uacaaaaaau ccuuaaaaaa cuagugaaga 120
uuacuaaagu gcaauagaua caaaaacaau auuuuuacgu auucaccaua auaauauaaa 180
cguagaaaau gaaucccgag uaugauuauu uauucaaacu ucugcugauu ggagauucag 240
gaguaggaaa aucuugucuu cuacugagau uugcagauga uaccuacaca gaaagcuaua 300
uuaguaccau uggcguagau uuuaaaauca ggacaaucga uuuagaugga aagacaauua 360
aauugcaaau uugggauaca gcaggucagg aaagguuuag aacgauuaca ucaaguuauu 420
accgaggagc acaugguauu auuguagugu acgauugcac agaccaagau ucauucaaua 480
acguuaaaca guggcucgaa gaaaucgacc guuaugcgug ugacaaugua aacaaauuac 540
ugguagggaa uaaaagcgau uugacaacua agaaaguugu cgacuucacu acagccaagg 600
aguaugccga ccaauugggu auaccauuuu uggaaaccuc agcuaagaau gcaaccaaug 660
uagaacaggc cuuuaugacu auggccgcug aaauaaaaaa uagaguagga ccuccaucuu 720
cugcgguaga ccaaggaaau aagguuaggu ucgaucaaag ucgcccaguc gaaacaacca 780
aauccgguug cugcugaaaa uauuuaguuc ucuuugguug aaggugguca cucaugucau 840
cucuuuugua cgguagcagc gcuacaguua cucuuauucu aguaauaucc gucuguaugu 900
auaguuauuu uaaaguuuuu gauuugauuu ucacuuuaga uuuuuuuuuc aaaaauauag 960
aaaacaccaa uaucucuuag ccuaaucuua acucccuuag auaaauaugu aauuaacaaa 1020
aauauuuaua aaaccauuuu uacuaaacua uuaguccaca guauaucguu ccuuuucaaa 1080
aaugauuaca aaagucuuaa aaaugguuuu aauuguuucg uuuaaacgag ucuaccacuu 1140
uaugaaaaau uucagaaaga uuauacguua aaauaaaaua aauuauuuuu gucggacaaa 1200
aaagaggggc aaacacccgc aagaugucac ucuagcgcgg uacaguuuug uuaauuuauu 1260
aguccacagu auaucguucc uuuucaaaaa ugauuacaaa agucuuaaaa augguuuuaa 1320
uuguuucguu uaaacgaguc uaccacuuua ugaaaaauuu cagaaagauu auacguugaa 1380
auaaaaugaa uuauuuuugu cggacaaaaa agaggggcaa acacccgcaa gaugucacuc 1440
uagcgcggua caguuuuguu aauuacaaaa cucauaauuu ucaauugaaa guauugauaa 1500
uuacuaaaua uuguggaucu cuuguccgac agauuugcag ccucguuucu uuagugggac 1560
agcggaaaua uguuaauuau gguagguuau gguuugcggu uacaaaaguc auaauuuuaa 1620
uuuuuuaacg uucaaacugu ucaaaaagua auuguccgac aaaaaaauug uucgacgcaa 1680
acuuauaaca aaaacaguga cagucuacuu guguuugucg guauaaucgc aaauuaccua 1740
ccucuaguau agaugcuuag ucuaguaauu agucuguucc gcucugcgga auugaguaaa 1800
uggaauuaau uaaaaauaag uguaaacuau gaacaauaau auauuuauuu gugucgauaa 1860
aaagacgugu uuugcucgaa aggguguguu guugacgcac cgcaucuauc ccgacgcccg 1920
cuggggugug acugccgacg ggagguugua aaucgacccg ugguuaugua ucgacugacg 1980
cuguggcuga aaaaaaccac aaacgcuaau auguuuguac uucuaacauc uggccagaaa 2040
agcgauaaau uacuacaucu guaguuuuaa gaaacgucuu cgaaugcauu uccaugcauu 2100
gacauggaau cugauaaggu aauuaauuca uggggaauuc aguuuguuaa acugaacaca 2160
ccaaaaauau gguuagggau acauuucguu ucguaaaacu ucuuagcauc ugguuuguau 2220
auuaaaugug aauuuauaug accguuuuga gaaaugauau uaaaaaauaa aauuagcaaa 2280
aauaguaauu aaagcguguc gccacaaguc uauguuuaca uucgguuucu auacauuuuc 2340
ugacguucua gacgucacua gacauaaaac uaaacagugc aacaaguaaa ggguccaggg 2400
cuauguuagc ucaauuuauc uaguacugug gcgguugcag uaaaaaaagu uuugaaauua 2460
auuugucgga cuggcuaucu gauacccaau uuuugucgga cauugcucua uuuggauaaa 2520
gaacccucca aucuaucacu uucguguucg aaauuuuaua aucgcaaucu gcauaauugu 2580
uaauuauuaa cauauuuacg uuguucgacu aaagagggga ggcugcgaau uugucugaca 2640
agagaucaac aaucuaguaa uuaauuauua auaaacuaaa aguggguagu ucccuggguu 2700
ggcuguauac cauguaguua aaaaacucua aacauguagu aaaaccacuu cuauauaaaa 2760
gguauaugua cugaaauuuu aaaaaucccg auggauugaa uaaacuaggu ucauucagaa 2820
cguuuucgga ccugucaguc caucaucagu gaauucauau auacuugcgc uaacuagucc 2880
cagaaccaaa caaugguugu acuuauaaau caaucuacau uauaguguua uguauuugga 2940
aaggcuaagc gccgauguuc ugauggacug auagguccga aaacguucug aaugaaccua 3000
uuuuauuuaa uucauuggga uuuuuaaaau uguaguaaau auaccuuuua cacagaagug 3060
guuuuauuuc auguuuuuug uccgacgaaa auaauuuauu uuauucuaac gcguaauuuu 3120
uugauuaaaa uuaaaaauua uaguuuugca aucucaaaac aaccgcgcua gagcuacagg 3180
uuuaaaagga agucaucaaa accauuuuua agacuuuugu aaucauuuuu gaaaaacgac 3240
gauauacugu ggacuacaua uaugagauca uuagaacgag aaagauauac ucaucgacug 3300
uuaauuuugu auuguuuuua guauaccuuu cucuguuuua uaaauccaga ucaugauaga 3360
uugacguuag aaauuauugu ucgcuuuuca aacguguauu agcagaauuu uggcaaauua 3420
aauauaaaaa uucgagaucc uuuauaaaaa gguauauuua aaauucccua aguaaggguu 3480
auauuaagua acagaacguu uucggauuaa aaaauccacc aucaguguua caaaaaaaaa 3540
auagcaugcc ugaggcaccg aaauguuacg gguaaaaacc cuuuaaaugu ugacaguugu 3600
ugucuuauac uaugauguug cuaauauucc uggauauuac ccagggcaac acaggacucu 3660
ucccacgugg uugaaauuug agaauuucaa ucacgaauuu gacacuucca aaauacguaa 3720
aguuuuccac aucuuuaauc ugcauguugu gaauaguaaa uagagugucg uuccuugcau 3780
ugguucucau ggacuugauu uuacuaauau uaauuuucaa aucuauuuua uuggcuucag 3840
uggaaaguga auuuugaaaa aaaaucugcu aaaaauaguu uaaaaaauau uuuuuuuuag 3900
uugugacacu auacagauaa aauaacgaaa aauuuacgaa auaauauuua ucaaaagugg 3960
cucuugcagu acagaauaua auaucauuau uauuaucuag uuaauagacu cguacuuuuu 4020
aacgauguaa cuuuaaaaac aauuaacaac guuaaaaaaa uacugcaaaa aggaagauaa 4080
auaagugaua aaugugucac uuuucuugag cacuacgcag auuguuugga auaaagcuau 4140
cacguauuau acuguguaaa cuccacaaac gacacuuuau acacaucuua gaugaacaaa 4200
ugccuggcac aagucgauau gugacacugu uuauguucgg ugcaccguca guuuugguua 4260
aucauuagug ucagcuaacg aaaauacuuu aacuauaaac uugacacuuu uugagauaag 4320
auuuuguagu uuuauuaaac uguuggugca auaaaaccgu uuuuaaaauu uuuugaguug 4380
ugacacuauu ugagcggagg ugcgacaugu ggaaaauauu uacaauuauu ucauauauca 4440
cuuguucuau acaauaaaaa aacgucauuu cuaacaugau uuuuagugau uucuguuggu 4500
ccaaaacaau uuauuauauu auccauauau acuuguuauu uuuuauguaa ugauuuuucu 4560
uauucgcuac acauuaacgu cauuuuuugu uuauaucaaa uaaaaaguaa uauuguaagu 4620
aauaugugau uguaaaucuu ggucaaua 4648
<210> 202
<211> 1577
<212> RNA
<213> 玉米根萤叶甲属
<400> 202
agaaaacaag cgguuuauuu gccgccauuu ugacaagcuu acguaccuac auuuuuaauu 60
cguuguuuua uuuuuaguuu ucaauaauac ucuaaaaaau ggcagacgcu gaugaucuau 120
uagauuauga agaugaggaa cagacagaac aaaccgcaac ugaaacggca acuacagagg 180
uacagaaaaa gggugucaag ggcacauaug uaucaauaca caguucuggg uuuagagauu 240
uucuguuaaa accagcaauu cucagagcua uaguggacug cggguucgaa cauccuucag 300
aaguucaaca ugaauguauu ccucaagcug ucauuggcau ggauauucug ugccaagcua 360
aauccgguau gggaaaaacg gcuguuuuug uauuagcuac acuccaagua auagauccua 420
cagaaaaugu uguauauguu cucgucaugu gccauaccag agaguuagcc uuccagauaa 480
gcaaagagua cgaacguuuc aguaaauaua ugcccaauau uaaaguaggg gucuucuuug 540
guggcuugcc uauccagaaa gaugaggaaa cguuaaaaaa uaauugcccg cauaucguug 600
uggguacucc aggaagaauu uuagcauugg ucagaucgaa aaaacuuaau cucaaacauc 660
uaaagcauuu uauuuuggau gaaugugaua aaauguugga guuauuagac augagacgug 720
auguucaaga aauauaucgu aacacucccc acgaaaaaca agucaugaug uucagugcca 780
ccuuaaguaa agaaauuaga ccaguuugca agaaauuuau gcaagaugua auucaaaauu 840
cuuauaauac acaauuuugu aaugacgcac ccacucgcaa uguuugaaaa guucuaauuc 900
aaaauacggg ccaaaguuuu auuaaaucga aucauuuaaa agaugagggu guuggguuua 960
aaucauuagc gcauuguagc ccaacgagua ggcaggggug ggccgggcca agaagaggac 1020
acuauacagg auuuucuaaa uuuccguaaa ggguuccgcg accacauuuu cggccgagcu 1080
acaauguaga aaaauuuaca cacccuccaa guccaauaga auuaaugggg uuaauguuug 1140
aaguaccgau ggaguucuuu guugauuuau uuagaagagu auuuuuggau aauuguucag 1200
agaucauuuc aauggugaug cagcgaugau gggaugcgag auaucaacug acuguaugua 1260
cgauggauuc agucaagacu ccugagccuc guaacugcag acuauuucau uauuuuauua 1320
acuaaaguaa uaauugaguu uuuuaucgcc ucaauuauua uuucaaauaa uuuauauaaa 1380
gauuuagaaa acuaugauuu uguugauggu uauauuuaac ugucggugag uauuuucauu 1440
cacaaaagug uguguguuau ugcguugccg cuccugaaau aucuagaggc caccuaucga 1500
uggaucaaga guaaacuacc uuuauaguuc auaacauuua aauuagaaug auauaauugc 1560
acauuaaaac auaauua 1577
<210> 203
<211> 1039
<212> RNA
<213> 玉米根萤叶甲属
<400> 203
gcaucugguc ugcccgcaua agcuuguugg aacugccagu gaguacacag ugauguucac 60
agggaagaca gugaacggug agguguggag uaaugagaau caugaguuuc aucaggugcu 120
aaugcaacuu ccaacaaaaa cagauuuuuu ucguaaaaug ugaacguguu agucagucaa 180
aauagcauca aaaugccggu cauugauggu uauaaaguac uuuacauuuu auuacacagu 240
uuauauacaa uuuuugaaaa uauuuggagg acucuuuuau uuauuuauca aaauuguaua 300
aggguuauaa acccugaauc uacauucgau gaugcugacc aguuaaagaa aagacugucu 360
agacuaacaa aaaagccuca acauuuaacu aucauuauug guguggaaga auauucauug 420
guagauuugg cuaaccucgu auauuggugu uuaggucuua auauuccgua cguuaguuuc 480
uaugauuaua aagguaauuu aaaaaagcau gaagagaagu ugcaacaaau uguagaaucc 540
agaaaaucag agaauaucaa cauaauuugg cacacccaug cagaacaaag gcauaaaaau 600
ggauuuuugg guccaaaaau ccacguaaaa guguuaacac acgcggacgg aaagcaaagu 660
auaguaaaug uuacuaaaaa auuagcucua aauaaagaaa aagacauuag uaaagaaaaa 720
auuagugaau uacuauuaag gcaguaugaa uuuccagauc cagaaauggc uauuauuugu 780
ggaaagaaac ugaacauuua uaauuauccu ccuuggcagu uaagacucac agaauucuuu 840
aaagucaaca aagucaacaa caucacauuc ccaguguuug uggaaaaauu ggaaaaguac 900
agcaaaugug aacagagggu gggaaaauaa uuguuuuaua aaaaacuguu uuuguuuugg 960
uuaucuuuau uauuaagaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1020
aaaaagaaca gaggguggg 1039
<210> 204
<211> 697
<212> RNA
<213> 玉米根萤叶甲属
<400> 204
cucgaauaaa cgccacaucc aaacaagagu ugacguugac agcgacauuu ccuccgccau 60
uuccuuuuac uguguuucuu aggauuugga agguacaaaa ugaccaacuc uaaagguuac 120
cgccgaggaa ccagggaucu auuugcccgc aaguuuaaaa aacguggugu aauuccacuu 180
uccacauauu ugagagucua caaaguugga gauauuguag auaucaaggg uaauggugca 240
guucaaaagg guaugcccca caaaguguac caugguaaga caggacgugu uuucaauguu 300
acugcacaug cauuaggugu aauuguaaac aaaaggguuc gaggaagaau cauccccaaa 360
agaaucaauc uccguauuga acauguaaac cacuccaagu gucgucaaga cuucuugcaa 420
agaguaaaau ccaacgaaaa gcuacguaaa gaagcuaaag aaaagaacau uaaaguagaa 480
cuuaggagac aaccugccca accuaggcca gcacauauug uuagcggaaa gguuccagca 540
caggugcuug cuccuauccc auaugaauuc auugcuuagg uuuguuuauc uuaaaauaaa 600
auccuuuaua uaauaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 660
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa 697
<210> 205
<211> 784
<212> RNA
<213> 玉米根萤叶甲属
<400> 205
cugacuucaa uuaaauggcc ucugucaaug ucagaguaaa ugucaaagug uaaaaaauaa 60
aaaaaguaac aacacaacag auuuuauuaa guuguagacg uguaauuuuu guaaauuuau 120
ucuaagccau uauuaauuaa aguaauacua aaauggaagg aauacuacug gaaccaacau 180
uguauaccau aaaagguauu gcuauauugg acuaugaugg uaauagagug cuggcuaaau 240
acuacgauaa agauauauuu ccuacagcaa aagagcagaa agcuuuugag aaaaauuugu 300
ucaauaaaac ucauagggca gacgcagaaa uuaucauguu ggaugguuua acuugugugu 360
auagaaguaa uguagauuua uucuuuuaug uuaugggcag uucacaugaa aaugagcuaa 420
uuuuaaugag uguuuuaaau ugcuuguaug acucaguaag ucaaauauug aagaaaaaua 480
ugcaaaaacg agcugucuug gaaucacuag auauuguuau gcuggcuaug gaugaaauug 540
uugauggagg aauaauuaua gauucugauu caaguucagu aguaucuaga auagcauuaa 600
ggacugauga uauuccauua ggagaacaaa cuguagcuca gguauuccaa acggccaaag 660
aacagcugaa auggucauug cugaaauaaa gugcguauuu uaaaacaagg uaaucgguau 720
uuauuucaug uacaauuuaa uuauuaagug uaaauaaauu uuuucuguuu aaagaugaaa 780
aaaa 784
<210> 206
<211> 1850
<212> RNA
<213> 玉米根萤叶甲属
<400> 206
guuugacgca uugcagguac aagccuacca aucacaaaca cacauuguac gaguagacgu 60
uguaaacaag ccgcacccga uguucaaacu ugacauaaca uacgccacgu caacuagauc 120
gcccaacacu acacaauuuc ccucacgaaa aaccaccgca uccaacacag uuuaaaauug 180
uacagcgaaa auaccaucuu caauaguuug uaaagcuggg aaaacaaaua ggaaaauuua 240
aacguuucgu guguuggcgu aggaauauuu cuaccgguuu cugucauugc cgcacuauug 300
cgcuuuggug guuucgcagc agcaguuccc cccucucuuc ucuuacgucg auccuaaaca 360
aaacgagaaa agacaguugg cuggugcacu uugacgagag gcguuguuag uuacuucguu 420
gugucgacau uuuucuguuu aguguaccga uuaaucuuca aauauuacaa uggcugacca 480
acucaccgaa gaacaaauug cugaauucaa agaagcuuuc ucacuauucg auaaagaugg 540
ugaugguaca auuacgacua aagaauuagg aacaguaaug agaucucuag gacaaaaucc 600
aacagaggcu gaauuacagg auaugaucaa ugaaguagau gccgauggua acggcacgau 660
cgauuuccca gaauuuuuaa cgaugauggc acguaaaaug aaagauaccg auagugagga 720
agaaauucgu gaagcauucc gaguguucga caaagacggc aaugguuuca ucucagcagc 780
agaauugcgc cacgucauga ccaacuuggg ugaaaaauug acagacgaag aagucgauga 840
aaugauucgg gaggccgaua ucgaugguga uggucaaguc aauuacgaag aguucgucac 900
caugaugacu ucaaagugag gaaaccagag uugcauuuuc agcuuuccau uguuucaucc 960
cggcucauug ucuacauuuu ucaacaccuc gaacuuuuug cuuuguggcc ggguccacua 1020
aagugaauaa cuuaaccguu auuugauuua caagacaaau uuaauuaaua aauauauuua 1080
uaaauuaaua uaauugugua aauuuuugua auauauuugu uuuucuuucg caggagucgu 1140
uggcgagauc uagucgcugg cgauucuugu acuguaucaa auucugugcu acaugucuag 1200
uugaaguuua aaaagaguua caaagucaca aaguaugaaa acacguauau auaaugaaga 1260
uaaaaaaauc guucuauuug auguuaguuu aggggauaca gcauuagaca cuuucaauuu 1320
uuuucauacu uugugaugua aauuacaucu gaucugugca cuuacuauau aguauguauc 1380
cccuuuuuug acauguucau ucauuuuucc ccuuucuugu acuguagguu uuacauuuuu 1440
agucuaggau uugaauuugc uggaaaguaa gcacauuuuu auugucaucc ugugaugcgu 1500
auggaaaugu uaauauuuuu uggcucuuac uguaucacag gaaauggucu uuagaaauug 1560
aucgaaauuu uuuacaacaa aaauuuuauu cuaauuucuu aagaccacuc ccuuucuuau 1620
uguaagauuu cguuuuuauu caagccuguu aauuuuuuua uuuauucuua cuauuuaaga 1680
uccaguaacg cauccguuac aaugugaaau uauuagaugu uuaaauuuga aauaaaugug 1740
acuaaaauau uuuccuguga cuuacuggua cccaauauau uauuacuaua aaguuuuuau 1800
guuagaauuu uuuucuuuau uuaaaauaaa auauuugaau uaaaaaaaaa 1850
<210> 207
<211> 3245
<212> RNA
<213> 玉米根萤叶甲属
<400> 207
cagcgccauu uuaguauuua gcucugaaga uugaguuuuu acuuccguga aaaauuuuua 60
cgaguuuuua uugucaucgu uauaauauuu ucgacaacug caguucugua uauagucggu 120
uugaaaaucu uaaagaaaau aaugagggua ugaauuuaaa cgugcuccaa uauuagcgcu 180
uagcguguau uugccggugu auuguguuca acgucaccau caaccaugau gcaagcaaac 240
aaucgagucc caccuauaaa guuggaaaac gauauagauc uuuacgccga ugauaucgag 300
gauuucgcac aagaugacuu ugguggugaa aauguugauc uauaugacga uguaauaucc 360
gcuccuccug gaaauaauga caacccaggu gauucaaauc aucaugcucc uccuggugcu 420
ggugaagaug guggagguaa uuuuguuggg ucaggaggag cacccaauaa uauaaauucu 480
ucuggaagaa gacaucagcu guauguugga aaucugacuu gguggacaac ugaucaagau 540
auagaaaaug cagugcauga uauaggggua accgacuucc augaaguuaa guuuuuugaa 600
cacagagcaa auggucaauc caagggauuc ugugucauau cuuugggauc ugagggaagc 660
augagacucu gccuggaacu ccuaucuaaa aaagagauca auggccaaaa uccccuuguu 720
acccuuccca caaaacaagc ucuuaguaac uuugaaaguc agucuaaaac acgcccuucu 780
ccuacuaaua auucuaacuc acguccuccc cauccuaaua auaauguuca uucagguccu 840
augcagaauu auggagguag aaugccuaug aacccuucca ugcgucccau gcccccaggu 900
augcaaggug cuccaagaau gcagggucca ccuggauuua auggaccacc aaacaugaau 960
cagcaacccc ccagguucca agguaaucca caauggaaug gaccuagacc uaaugguccu 1020
gggcccaaua ugggaaugag acccaugggg ccaccucaug gacaacaagg gcccccaaga 1080
ccaccaaugc agggaccacc gcagcaaggu ccuccaagag gaaugccgcc acaaggucca 1140
ccgcagaugc guccagaaug gaaucgacca ccaaugcaac aaggguaccc ucaaggcccg 1200
ccgcauaugc aaggaccuaa caugggucca agagguccac cccaaauggg accacccggg 1260
gcgccucaac agcaaggacc agcuccgcac guaaauccag cauucuuuca acaaggagga 1320
ggaccaccgc ccccaaugca acacaugccu ggaccagggc ccgucaugcc uccucaagga 1380
cccccgcaag guccaccaca cggacccguu ggaccuccac acggcccacc auugggucca 1440
gcgaauguuc cgccucaugg accaccucac ggauaugguc caccugcagc gaugccacag 1500
ccgccauacg guggcccacc uccagaccac cgcgcugaga uuccucaguu aacagagcaa 1560
gaguuugagg auauaauguc ccggaauaga acaguuucca guucggcgau ugggcgggcc 1620
guauccgacg ccgcagcugg agaauuugca agcgccauug agacuuuggu uacugcuauu 1680
ucacucauca aacaauccaa aguggcuaac gacgaucguu gcaagauccu uauaaguucg 1740
cugcaagaua cuuugcgugg ugucgaagac aaaagcuaca gcuccagccg cagagaccgg 1800
ucaagaucca gggacagauc acauagaaga acuagaagag aacgauccuc gucacgguac 1860
agagacagaa gcagagagag ggagcgugaa cgcgauagag aucgugaucg ugaacgugac 1920
agauauuaug auagauacag cgaaagagaa agagaccgag aucguucaag aagcagagaa 1980
agaacagaaa gggauagaga acgagauuau agagaccggg aacccgaaga gacagauaaa 2040
gaaaaaucua aaguauccag agucucaaga ucaagaaaca aaucuccgga accugucgaa 2100
ccuagcagcg agguaccgaa aucaucccgc uauuaugagg auagguaucg ggaacgagag 2160
agagaagguc gacgagagag cgaucgcgaa agagaaagag auagaagagg ggaagacagc 2220
cauaggucuc gacacuagca uauaguuagc gguucacaga aacaacaaca aacaaguuau 2280
aguuggaguu caaacaaaga uuaucguguu uaauuuagag auagguuaua auaugugaug 2340
uuacuuuaca uaaauuuaaa cagguuccga auguguaagu uaaaagagca aaggaaacau 2400
ucacuaacuc auauuuugcu acuuguuuca uuuugcaaug gaacuucugg cuaucuuaaa 2460
uguauucaua aaaaggauaa aaaacauuua ccuguuuauu guuguauuaa guaaaucauu 2520
gacuauaaua uugcgauaaa uguuagauau uuuacuucau gggguuauac uuuugaugua 2580
aaaguuucac auauuuagcu uuaagcaaac gugacuuacu augaauauaa uaaauuaugu 2640
aagaacaaaa uuaaguaaug aauauaccac auaauucuuc aacuaaugag uuacuaugua 2700
caguauauuc uucuuacuau uucuucaguu uucuccugga guagcaaaac uuguuuuugu 2760
aaaaugaauu uauuguuuag gcugugcuau auuaacacug ggucgucuau uaaucuguua 2820
aaaaacaguu uuugagagaa uucuguucuu uauaaaaaau uacaggaggg ugaugaaagg 2880
cuuuauuguu acgugcaugu ugguaaaucg ggaugcuucu aaaugauaaa uaacuuuagg 2940
caacuuguug uuucugugag cuguauuuuu ucacauuuuu auguaaucau acauaaguaa 3000
aucauguaac guauaguuga uaaguacaau auagauggaa aucaaguuuu guuuuugugu 3060
guaguauaug gauuuaaaac auguaucuuu uaauaagaug auguuucaac auacaaugaa 3120
uaauuugugu aauaaauuga auacuauuca acaugaauug ucaaauucuu acaaaaaauc 3180
augauaauuu gaaggcaauu gaccaugaac uacucacaau gcaucuaaau gugaguguau 3240
ccucc 3245
<210> 208
<211> 1023
<212> RNA
<213> 玉米根萤叶甲属
<400> 208
gucgucaguc gucaacauca auuucaaguu ucaagaaaaa gcaaaucacu acgacuugcc 60
ggauuuugua guaguguuaa uuuuguauua aaaaaucaaa augaguucua uuggaacugg 120
guacgauuua ucagcuuccc aauucucucc ugauggaaga guauuucaag uugaauaugc 180
aaugaaagca guugaaaaua guggcaccgu aauaggccuc cgagguacag auggcauugu 240
auuggcugcu gaaaagcuca uuaugucaaa auugcaugaa ccaaguacaa auaaacgaau 300
uuucaacauu gauaaacaca uaggaauggc auuuucaggc uuaauagcug augcaaggca 360
aaucguugag auugcuagaa aagaagcauc aaauuauaga caucaauaug guucaaauau 420
uccucuuaaa uaccuaaaug auagaguaag cauguacaug caugcauaca cuuuauacag 480
ugcuguuaga ccauuugguu gcagugucau cuuggccagu uaugaagaua gugacccauc 540
uauguaucug auugauccau cuggaguuag cuauggauac uuuggaugug cuacagguaa 600
agcaaaacag ucugcaaaga cugaaauaga aaaauugaag auggggaauc uaacaugcaa 660
agaacuuguu aaagaagcag ccaaaaucau uuauuugguc caugaugagc ugaaggauaa 720
gaauuuugaa cuggaacuuu cauggguaug caaagauacg aaugguuuac auaccaaagu 780
gccugaauca guguuugcug augcagaaaa agcugccaaa caagcaaugg aagcagauuc 840
agaaucagau acagaagaua uguaauaacu acauuuaguu uuuaauauuu cgcugauggu 900
ggcuguucuu acaauauuuc guguguuaug uucauauauu auguaauacu gugagaauuu 960
ccauuucaag gauagguuua uaacuuuuuu uucuaauaaa uacauaacuu uaaaaaaaaa 1020
aaa 1023
<210> 209
<211> 1439
<212> RNA
<213> 玉米根萤叶甲属
<400> 209
aaagugucag gugcuguugg ugucagcuga cuuuuuacuu uuuguuccag uccuauauuc 60
aacacacuuu ggauauuaaa uauuuuauuc uaaaucucaa gaauggcuuc aaaagacaga 120
uugaugauuu uuccaucuag aggagcccaa augaugauga aauccaggcu aaagggagcc 180
caaaagggac auaguuuauu aaagaagaaa gcugaugcuu uacaaaugag auuuagaaug 240
auuuugaaca aaauuauuga gaccaaaacu cucaugggug aaguaaugaa agaagcugcc 300
uuuucuuuag cugaagcaaa guuugcaacu ggugacuuca aucaaguugu ucuucaaaau 360
gucaccaagg cucaaauaaa aauaagaacu aagaaagaca acguugcugg uguuacuuua 420
ccaguguuug aaugcuacca agaugguaca gauacauaug aguuggcugg uuuggcuagg 480
ggaggucaac aauugacaaa acucaagaag aauuaucaaa gugcuguuaa acuguugguu 540
gaauuagccu cuuugcaaac uucuuuugua acucuugaug auguaaucaa aauaacaaac 600
agaagaguca augccauuga acauguuauc auuccaagaa uagagcguac uuuggcuuac 660
aucauauccg aacuggacga guuagaaaga gaggaguucu auagauuaaa gaagauccag 720
gacaaaaaga agaucagcag agcaaaggcc gagaaacaaa aacaagcucu ucuccaagcu 780
gggcuacuua aagaguccca ggcaaacaug cuuuuggaug agggcgauga agaucuacuu 840
uucuagaaca ucaaacagcc ugaagugugg uucuguacau augaauaaau auauaacgcu 900
aacuuguuuu uagacgguaa cuguuuauuu uucgcauuaa uuaauacauu uuuaagauau 960
aucuuuauuu uuaacugguu uuuauucuua ugccuugcaa uaguaaaaga uauacgaacc 1020
cggauauuuc cuauauaaau aacuuccuac uuuuuauuaa cuccaguuuu uaggauuuua 1080
auacaauauu caacacaucg ugcaauagaa uuugaaguga auauuacuug cgaaaauuaa 1140
aaagguacca aauauuuuuc uuaauauuga acguauauuc cagaauagua uuauaaaagu 1200
uuugauugaa auucuuguac gugacacuau gaacuuguag auuuuagaga aagcagcuuu 1260
ucaauggaaa augcuuuauu gauauggaca cuagauaaug uaaauacuug uuaauauacu 1320
uugaacagau aaaauaguuu auucguuuua aauucuuuaa aacucuaaau uguuuuucau 1380
caaauguaua cauggccugu aaauuguugg uuagaaauaa aacucuguuc aaaaaaaaa 1439
<210> 210
<211> 5226
<212> RNA
<213> 玉米根萤叶甲属
<400> 210
gcgauugcgu cugcugaaug cacccauagu auugugguga cugugacagg ugacagucgg 60
agucgacagu gaaguacaaa ucaaaaucau ucccuauuug ucaaacaaaa augaaauuac 120
guguauuauu auuuacccaa guaacuuuuu aauaauuuaa gccgauuauu gcacuguuag 180
uacguaugua caccacaaau aaccaauaua uucuuuguuu cccaugauaa cacaguucua 240
auuucacugu uagaugcaug uuacauguuu uuaguggaau cauuacugga uaaaguccuc 300
ucccauaauu aaauaaauac uuaaaaaaug gaggcggcuc ccaaguuacc gaugcucucg 360
uucgaguuaa auacuuguac agagaacguc cacuuuggcc cccaguuaaa acaguauauu 420
gcugcuuuuu auggugaaga uccagaaucc uacauuacag aaaucagcaa ucuugaaucc 480
uuaagaucag cugcaguucg accaucaacg gauguaaaug guguacaacu guugaaaaag 540
uauuucuguc agcuucguuu ucucaaaucu agguuuccca uggaagagaa ucaagaugcu 600
gcaguucuau uuucauggaa aaauaaugaa uuagacauaa cuucaacauc cagugauauc 660
agauaugaau uaaugguaau aauguauaac auuggagccu uacacacuuu ucuuggagcc 720
aacgacucaa gaaacaaucc ggaugguaug aaaauggcau guacucauuu ucaaugugcu 780
gcaugggcuu uucaaaacgu aaaagaaaag uaccaccaau ucauaucaaa caucucauug 840
guagaacugg uucauuuuuu ucaacaaguc uguuuagcuc aggcucagga guguauauua 900
gagaagagca uguuugacaa uaggaaaccu accaucauug caaaaguugc uauccaaguc 960
uacaguuauu acagacaguc uuuacguguc uuggaaucag uaaaugaagc cuacuuuagg 1020
gauaaaaccu acaaggagug gaugaaauau cuucaauuca agcugaccua cuacaaaugc 1080
aucucguucc uauuccaagg gcaacaagcu gaggaacaac agaaaauggg agaaaggguu 1140
gcauucuauc aagcugcaug ugaacagcug gacgaggcaa agaaaauugc ugcuacauua 1200
aaaaaccaac accaccagca agaaauaaau gagggacuag cauucacuac ugaugugguu 1260
gaagguaaaa gaaaagcagc uaaaaaugaa aaugaguuca ucuaccauga aucagugccu 1320
gauaaagacc aauugccaga gguuaagggu gcuucauuag ucaaaggaau accauucagu 1380
auaaaugaua uagaaguuuc aggaccagau auuuucuccc gauugguccc aauggaggca 1440
cacgaagcag cuuccuugua cagcgagaag aaagcucaga gauuaagaca gaucggggaa 1500
cuuauugaaa auaaagauca aacauuggcu gaauuuaugu cgucaaugca gcuagaucua 1560
uugaccaaga ugcaccaggc uacuggaaua ccgcaggagu ugauugauag agcagcggcu 1620
cuaucugcua aaccuaacgc cauucaagau cuuauaagug cuaugggaaa gcuaucuaau 1680
auauaccaag acguugaagc aaguuugaau gagauugauu cuuuauuaaa ggccgaagaa 1740
caaagugaac aaaaguacca agaaacgauu gguaaaagac caccgagcau uuuagcuaca 1800
gauuuaacua gggaagcggc aaaauacagg gaggcucaua cuaaagcgaa cgacucaaac 1860
caaacuuuac acagggcgau gauggcucac guggcuaauc ugaaaauacu ccaacaaccg 1920
cuaaagcagc ugcaacauca gcugcccuuu gucgaguuuc caaauccaaa uaucgacgaa 1980
aaaucuuuga aagaucugga agcgcuaguu gcaaaaguag acgaaaugag aacccaaaga 2040
gccaugcuau gggcucaacu ucgagaaucu auucaccaag acgauauuac aaguucccuu 2100
guaacgaaac aaccaaauca gucgcuggaa cagcuguucc agcaagaacu ucaaaagcau 2160
caaaaucuga uuucguugau ugaacaaaac accucggcac aagaaaacau uaagagcgcc 2220
uuagucgauu cuuacgcuua cgcuguaaau ucaagaaaau acauccaaga uauacuccaa 2280
aagagaacca caaccauaac gucacugaua gcaucguucg acucuuacga agacuuauug 2340
gcaaaagcua acaaagggau agaguuuuac ucaaaacuug aaacgaacgu auccaaguua 2400
cugcaaagaa uaaggaguac cugcaaaguu caacaagaag agcgagauca gaugaugucg 2460
acugcgcaag ugccucaaug ggagagucau acgucacuug ccgcuccuaa acugaaagau 2520
uacuuggacu ccaggaagaa gagugcugcg uauucggagc cgaguguuca accacaacag 2580
ccaacuuuaa guuacucagc ugcuauggau cugccuccug guauuaggcc gacuccaguu 2640
ggaucagaaa uaacggaugu accgaaaaau auucaaggug aaccacaagg uuauauucca 2700
uauaauuacc aacaaccuuc uguuccugcc ucacagaaua uugaugaaga gacuauuaaa 2760
aaaaugaacg cauugaugcc aggugcuaag acgucagugc cuagucagua cggauacagc 2820
aacuacauuc caccaacaua cccucaaagu gcguaccaac cagguaauca gucuuacgga 2880
aaagaaacuc cagauauuaa cucaccguac gacccuacca aggcguucac ggcuacuacu 2940
aacgcuuauc guucggugca gagcuccuca acucaaggau acguaccgua cgcagaaucu 3000
aacguuucga auguugacag aguuggauau ccuagcaggu aucaguacca acaaguaccu 3060
gagauagcua cuacuccagc ugaucccaau auuaaugcgu acuacccaca uggguacuca 3120
ccgagccaga auuuaccgaa ugcuaauacu caacauauua ccggccaacu gaaguaccau 3180
ucgguggagu acgcuucuuc ugugccgaac aacaucaauu auaacagcuc uaccuacucg 3240
ucgccgcuuu cuaauauguc uaguaccaau uccucaaauc cuaguaacuu gaauaauucu 3300
uacgaguacu acuaugaccc gaauaccagu aguggugcag uaccgaaugc uucaaagccu 3360
caacagucga gcgccagcuc ugcaaacccg aguaccgcua ugaacaacua caauuauuac 3420
uacaauacaa guaccagcgg uaguguagca gcggauacuu caaaaauaca acaacaacaa 3480
caguacccag guacucagau gagucaagcg caguacuauc ccgccaaugc caguuauuac 3540
ucaaccagua cuuacaauac caacguccaa ggugguacca aucccucgua cgcaacugga 3600
caaacauaua aucaagugac accagugacc ucucaaaaug uuucucaaaa uuacaacuuu 3660
aaccaaguug guucuggagc aggacaccag caucaguacu acucguccgc uaacgccgca 3720
guaccauccc aacaagcugu aaauaacagu ucauuaccaa acuacggaua cgaucaguau 3780
uacggcaaca acuauaauuc cagucaaccg aguaccuaca gcgcaaacca agcaccuccu 3840
gcagcacaag cugcuccaag uaauauuccu gcugccacca aauccuccuc uaauguggau 3900
cugcucagug gcuuggacuu cagcauaagc caagcuccuc uagugccuca acaaaacauu 3960
acgauaaaac cccaagaaaa ggaaacaaaa ccaccggcug uuucuucuga aaccaaaaac 4020
caagauccaa caccaguaac cacgcccaaa caacccacug gaccagaagu aaagcgcuug 4080
uacgucaaaa uccugccgag caaacccuua aacaacgaug augugaagaa auuguucggc 4140
caagagcugg acagguauga gaaguucgug gagaccuuga cccacaaaac uuugagcggu 4200
ccgaccacuc uggauauuaa auggaaggag auccaagacc agcaggauug cgagccgcag 4260
aagaagauca uuuccgucgc uagauguuau ccuaugaaga auagguuccc ggauaucuug 4320
ccuuacgacu uuuccagggu ggaguugugc gauaguaaag augauuauau caacgcuuca 4380
uacauuaagg auaucucgcc auaugcuccg ucauuuauug uuacacaagu gccguugucu 4440
ucaacuguug gugauaugug gacgaugauu agagaacaac aggucgaacu gauccucugu 4500
uugguaaacg acaaugagau cggugaagau auuuacuggc ccaaagaaaa aggcaguagu 4560
cuuaacauac uuaacauggu cauaacguug caaaacguua uaguuaaguc ucauuggacu 4620
gaaagacuga uagcgauaaa cuuaccugaa aaacgggagu cccgugugau aaugcaucua 4680
caauuuacau cguggccugg cagcuuguuu ccaacaaauc cugaaccguu cgucagcuac 4740
accuuggaau ccaucaaccu auaccaacaa cagaagacca acacccaucc gguggugguc 4800
cauuguucau cuggcauagg aagaagcggc cugcucuguu uacugacagc ugcuauguuc 4860
gaugcugcca acaaugcuaa cucgauacca gaucuuacag cuuugaguau caaguugucc 4920
aauugcagga agaauauucu cagagaucga gagcauuuga aguuugguua cgaaaguuuu 4980
uuggcguaua uuaggcauau aguuugugaa gauaaagcca gaaagaaacu gaacgagauc 5040
cagcccaagg uuaaggagga accacuggaa ccaccuguca uaguuccaga accaaauaua 5100
gauccuuuaa guacuuuaga cccauuuugg gcuaguaaaa gauaagcuuu acauaguaaa 5160
uauuuauaca augauguauu auuauuuuga auguuaucua caccuucauu aauauuaaau 5220
ucccug 5226
<210> 211
<211> 633
<212> RNA
<213> 玉米根萤叶甲属
<400> 211
uaccgccugc uacucucuuu cuuugaccuc cuccuuuucu ucuccuuugu ccggcuuuaa 60
cuugcguuuu cccggcucca gucccgagca uaccuucucc ggaguuuucg guucuuccgg 120
uucuuuccaa aguacugggg acucucuuuc uucuuugaau ccaaugacaa cucuuucuuu 180
cggcggcuuc uuaauuucuu ucuuguucuu gcguuucguc ggcuuuccuc cgcauaguaa 240
cuucuuucca cgccauuugg guuugaacag cuacugcguu uacuuccggg uaauuuuguu 300
cauacacucc cuauaguguc ugcauaacau cuggaucuuu uauucuuuaa acuggagcuu 360
uuucuucacc uuaagucucu aaaagucuag aggcuuaacu ugucgguuca uuugcuggaa 420
ucuccguuua agcaguuugg uuggaacuuc uuccauaggu uuaugcuuuu guuuaagcgg 480
uuugaaguuu ucuuccgucg acuuaaauug aaggcauugg uugaguuuca acaguucuuc 540
uuucuuaagu ggaaucuucu ucuucuguuu cuuuucuuug gucugaccag uuucuucccu 600
cugcuuuucu uccauguucu ccgacuucgu acu 633
<210> 212
<211> 603
<212> RNA
<213> 玉米根萤叶甲属
<400> 212
uacacacuuc uucuucaacg gcgaaaucag caucuguuac cuaggccaua cacguuucga 60
ccaaaacgac cccuacuacg uggagcacga cauaagggaa guuaacaacc ugcggguucu 120
guagucccac acuaccaucc uuacccuguu uuucuaagga uacauccacu acuucgaguu 180
ucauuuucuc cauaggaaug gaauuuuaug ggguagcucg ugccuuauca guguuugacc 240
cuacuauacc ucuuuuaaac cguaguaugu aagauguuac uugagucuca ucggggucuu 300
cuugugggac aagacaacug ucuucgagga gaguuggggu uccgguuguc ccuuuucuac 360
uguguuuauu acaaacuuug aaaguugugg ggucgguaca uacaacggua gguccgacau 420
gagaggaaca uacguagacc agcauguuga ccauaacaca accuaagacc acuaccacau 480
agggugugac aggguuagau acuuccaaua cgagaaggag uacguuagga agcaaaccug 540
aaucgaccau cucugaacug acuaauggag uacuuuuaaa acugacuugc accgaugaga 600
aag 603
<210> 213
<211> 2742
<212> RNA
<213> 玉米根萤叶甲属
<400> 213
uacggugaag cuaaucuaua uuuuucuuuc gauugucgag cgagucuggc ccauuuuaca 60
caccuagaag ugggaugucu uggaaccuac gacacaagag aaaugucgcc uuuauauuug 120
caaaccuugu ggcuuuuagu cguugaccaa uucugaaaac uucauacacu acauggacaa 180
gccugucgau ucaaaaacgg guccuucuug accuaucagu cacccagacu acuauacguc 240
uaagcucaaa aguuaauguu auggaaucua gcccauguaa gaaaacuccg aguaagccua 300
auacacucua cauaacagca ugugggaugu guuggaauau auaauuguuc aucacuacua 360
uacgaauagu ucgaaaccuu aacccuuuuu cguacccgaa cagucguuca aaagcuuccu 420
gugugaguaa uauaauacgu uuagcgguau uuagguuuuc uguuguugug uaaacgguca 480
cguagggauc uaucuuguaa cuuucauacc guuaacccuc gcaggugucg cuuaaagugu 540
gaucuuccag uacucuuucc gcaauugaca caccugauaa uagugccacc ucuauuugga 600
auaaauuaga guccgcgacu acuaucuaau cauuuuuaga cccuaauagu uuuguuuuga 660
acacaaguuu gaaaccuucc uguacgaguu uuacauuggc gacguacaaa gguaggucuu 720
gaaggacauc gagaaugacc uucacuucua ccaugacagu cucacaccgu acgguugugg 780
guauccaauc uuucaucgaa uuuaauaccg aaacuuucuc auaccugaua aaagacggau 840
uucccuaggu uauugcaccg uaacccaaua cuacuuccau cguaaaacca auuucaacca 900
ucucuucuug gucgacaauc auaccuacgg ucaccuccgu uuuaauaaac ccggucugug 960
agacuugaag uuguccguuu agaguuccgc aaucgacuuc cacgccuuua uucucuaccu 1020
cuugcggaag gucaaagaca uuuucuauac ccacgaacgc ucuauauggg agucuguuaa 1080
guuguguuag gguuaccggc aaaacaacaa cagacacccc uaccucuuau guauuagaug 1140
ugucguuacc gaaauucuuu guuucgcaaa ccaucgcgug uucuuaaaca cacccgaguu 1200
cuaaggucgc uuauacggua gucucuuagg ccuagaugau agucuuaaaa auucuuaaag 1260
uuucucuucu ucuuaaaauu caggcuaaaa ccucgacuuc cauauaugcc accuauggaa 1320
aacccucagu uuagccaaag accaaacuga aagauacuaa cccuuugaga gcuaaaucag 1380
ucuucuuagc ucuauguugg uuuucgucaa augaccaguc uaucaccauu uaaucauaca 1440
aaccgguguc uucuaucgau gaaauaagaa agaauacuaa gacuacuuca aguuuuucgg 1500
ucucuauugu uaguccaacg ccuacuaccu caucuuagcc gaaaguuaga agauccacuu 1560
uauuugcuua gucacgcuug accagagacc cauccgcuga caaaauagau gugcuuaaga 1620
caauuagcau aguugaugaa gcaaccucca cuugaccaau guuaacgagu aaaccuggcc 1680
ggaaacauac agaacccuau acacggauuu cugcuaucua auauggagca ucuauuucuc 1740
aacgcgcauc auucgauggu uaaugaagaa agacaagaac uuauaguuug acggcaguac 1800
ucuucucuga aagguugucg ucugucucau gaaggcaggu aaggauuccu cgugucuugc 1860
ucucaccgug uaaagaaucu uuucguuccg aaguuugucg uucgaaaccg gcauucaugu 1920
cuaggucucg ugucuaagcu cgaccgucau cguaaucucc uagaauuaua ucgguuuuga 1980
gaucgaguuc uucgcuuguc aggcguuuuc accuuaguug aucgucuuaa ccgucgacga 2040
ugauuauuac auucgcaucg guuccuuaca uacguuuuuc gcguucuaau accuccgaac 2100
aacgaagaac gaugcucgag gccacuacuu uuaaaucagg caugagaucc ucuuugcugu 2160
guucgacuuu cguuuguauu gaaucguaaa aacaguguga acaaucaucc acuaaauuug 2220
uuuacagauc uguaagaaua auuauggcca ucuaacgguc uucgacguaa aaagcggucu 2280
agaauggaag gacuauucua augucuucag caccuugaca ccuucugagu caauagaagu 2340
caguuaguuu uucgaccugu cucggaacgg cuaggauuuu ugaugcuuuu agacaaggga 2400
ccaaauguuc uccgccacca ucgagucuuu aaaaaccuug ucgucuuauu uccaaaucgc 2460
gggcguucuc aacgguggug guaaggagga uuagugcugu ccuuacaaca ucggcuucaa 2520
guucguguua gcuuugugcu acaugguagu aaaucaagaa gcaaauaaag uagucuuuau 2580
cuucguguuu guuccucaag acgauuugga cuucuuagaa guuuguaaua ugucgaccug 2640
guucuacugc ugcuauagcu aaaucuaaac cugccacauu uauagcuacu cuuguaacug 2700
ugcugccuau aguuguagcu acuacuaaac gacucacuaa cu 2742
<210> 214
<211> 1173
<212> RNA
<213> 玉米根萤叶甲属
<400> 214
cacaauaacu uuccguuuuu aguuuauuac gucuagaaac auuuuuguga gugaccauuu 60
ugguaguggg agcuccaacu ugguagucua ugguagcucu uacaguuucg auuuuaaguu 120
cuguuucuuc cauaaggugg ucuaguuguc ucuaauuaga aacgaccuuu cgucaaucuu 180
cuaccggcau gagagagucu gauguuguaa gucuuucuua gaugugaugu gaaucacgaa 240
gcagaaucuc cuccauacgu guagaaacau uuuugagagu gaccauucug guagugggaa 300
cuccaacuug guagucuaug guagcucuua caguuucgau uuuaaguucu guuucuucca 360
uaaggugguc uaguugucuc uaauuagaaa cgaccuuucg ucaaccuucu accggcauga 420
gagagucuga uguuguaagu uuuucucaga ugggagguaa accaugaagc agaaucuccu 480
ccauacgucu aaaaacaauu uugaaauuga ccuuucuggu agugggaacu ucaucuugga 540
agacuauggu agcuuuuaca guuucgguuu uaaguucugu uucuuccaua agguggucua 600
guuguuucua auuagaaacg gccuuucguu aaccuucuac cagcauguga gagucugaug 660
uuguaaguuu uccuuagaug ggagguaaac caugaagcag aaucuccucc auacguuuag 720
aaacauuuuu gugagugacc auucugguag ugggagcucc aacuugguag ucuaugguag 780
cucuuacagu uucgauuuua aguucuguuu cuuccauaag guggucuagu ugucucuaau 840
uagaagcgac cuuucgucaa ccuucuaccg gcaugagaga gucugauguu auaagucuuu 900
cucagauggg agguaaacca ugaagcagaa ucuccuccau acguuuagaa acauuuuuga 960
gagugaccau ucugguagug ggagcuccaa cuugguaguc uaugguagcu cuuacaguuu 1020
cgauuuuaag uucuguuucu uccauaaggu ggucuaguug uuucuaauua gaaacggccu 1080
uucgucaacc uucuaccggc augagagagu cugauguugu aaguuuuucu cagaugggaa 1140
gugaaccaug aagcaaauuc uccuccuuua auu 1173
<210> 215
<211> 1131
<212> RNA
<213> 玉米根萤叶甲属
<400> 215
uacacacugc ugcuacaucg ccgagaacag cagcuguuac cgaggccuua cacguuucgg 60
ccaaagcggc cacuacugcg gggagcacga cagaaaggua gguagcaucc agcagggucu 120
gugguuccac aguaccaccc auacccaguu uuucugagga ugcauccucu gcuucggguu 180
ucguuuucuc cauaggagug gaauuuuaug ggguaacuug ugccuuaaua gugauugacc 240
cugcuauacc uuuucuagac cguagugugg aagauguuac uugaaucuca ucgggggcuu 300
cuuguagggu aagaaaacug acuucgaggu gaauuggguu uucgguuguc ucuuuucuac 360
ugaguuuagu acaaacuuug aaaguuaugg ggacgguaca uacaacggua aguucgacau 420
aacagagaca ugcgaaggcc agcaugguga ccauaacaug aacuaagacc ucuaccacau 480
aggguguguc auggguagau acuuccaaug cgagagggug ugcgguagaa cgcaaaccug 540
aaccggccau cucugaacug acugauggaa uacuucuaga auuggcuuuc uccaaugaga 600
aagugguggu gucgacuuuc ucuuuaucaa gcacuguagu uccuuuuuaa cacgauacau 660
cgaaaccuga agcuuguccu uuaccggugu cgucggucga gguggaggaa ucuuuucuca 720
auacuugaag gacugccagu ucaguagugg uaaccauuac uuuccaaggc aacgggacuu 780
cgagagaagg uuggaaggaa gaacccauac cuuagaacgc cauaggugcu uugacagaug 840
uugagguagu acuucacgcu acagcuguag gcauuucuga acaugcgguu gugacaggaa 900
agaccuccau gguguuacau gggaccauaa cggcuagcau acguuuuccu uuagugacgg 960
aaccgaggua guugguaguu uuaguucuag uagcgagggg gucuuucuuu caugaggcaa 1020
accuagccac cgagguagaa ccggagggag agguggaagg uugucuacac cuagagguuu 1080
guucuuaugc ugcuuaggcc gggaccuuaa caaguggcgu uuacgaagau u 1131
<210> 216
<211> 2640
<212> RNA
<213> 玉米根萤叶甲属
<400> 216
uacccaugaa aauuuucucu augaguacua cuccugcccc cuaguucacg aaaaguuuua 60
gaccucuuuu gaugacaaaa cguccuucga ucucaaaaau uacuuugauc acauuuaggu 120
ucuuuuacau guggcuauga uuguuuugac aacaugaaua acuugguccc acuuugaaau 180
ucacgguuuc uccggugucu acaaaagaaa cgguacuggu uugacaaggu uaguuuucua 240
cauuauaacu cuuccuacca aauaaacccu uaauuucuug agucaagaca acgacuacua 300
caguaauaac auuguagguc agaauguuuu cuauacugac cauuucuucu guacaugucu 360
cgucgacgau auucucguaa uacgucauaa ugacuacgau gauacgaagu ucgauaucuu 420
gcaauauacu ucguucgaua acaucuaucu uugcgucgac agucaagucg ucgugauuaa 480
ucaaguaaug uauacucguu uaaucgaggu cuacaucauu uuucuaccca uuuacuucga 540
guccuucguc auuuaucacu auuacguuac caugucauag ugcguaaucc agaagauaug 600
guauaauccu ucugacuauu cgaucgucac uguuuuaacu aaagguucga cuuaaguuac 660
ccaaauuucu cgggaauacg aaacacauac aacuauucuu agugacguuu ugaaaaucuu 720
cuucuccugg uucucaguga ggaguugagg gguauauguu auuauaaaug uuacccgaau 780
uccuuguuua gacuuuacca ccacauacuu cgacguguac gguaccaauu ggacuucaag 840
ugcucaucau uacacgaucg ugggcgauau ucacaagaug uugauaaaac accuagagga 900
uuucggugug agucuaaacg acgacaaucu ugaaauuuag uucaccggug guggguggga 960
cgcagucacu gucgaacauu aaaccuagau cuuuuaaacu aaugacuagg auuauccagu 1020
uaacgaugug accgguaaug augagaaaac uuuuguccac ggcuuagaag acaacugucu 1080
gauuacuuug uuuagcgaug aaaacauaga cuuuagucac uacuuaaauu ucaccaacag 1140
uaaguccguu aauuccauaa ucgaaacuuu aaagguuccu uuguaucgug cgaauacuua 1200
aaggauaggc gguacaauuc ucuacucccu ccaaaucuua uauuucguag guaucgucua 1260
ugguaauauu gggauuagcu ucuauuaggg cuucgauuuc uuagaccaaa ccgcguagaa 1320
acgcucaagu aacuucugac acuuguacaa agaaaccgac acucuuagaa cguaaacaau 1380
ccuuuccuuc cuggguucug guuuguuggu agcucuaugu aggcaaaaua gauguuagcg 1440
caguauaacc uuacaggaag acauucucga cgacgucaga ggcgguaccg uguuaagccu 1500
cggagaacag ggcuaaacaa ucuuuuauag guuuauaaug aaagcuccac agucuaccua 1560
agucugcuac uucaaucccu gucucgaugu auaauaucau uauaugaauu guuuuuacua 1620
uuuucaaaua uguuguuaau guaaaaccua agaaacgucc aaaguuaagg aagugaucuu 1680
ucuagcgaau cucuuaugua aguuuuaggu ugacugcuug guaaacugua auucaggcau 1740
ggacaucguc gucacgguug ucgucuucuu gcucuucaau uuuuguuuag acuuccugac 1800
gaucagagag uuccagguca ggcuggagga ggccacagau cucuucuuuu gaagcggcuu 1860
uuugaaucau ugcaaggccc auauguuguc aauccuggaa acaaguuuug aaggcugcag 1920
caacuugagu gacuuagacu uugucucaua aaacaggcga cauaguucgu gacaaaguuu 1980
guaguguagc aggagguuaa gcuaacagac uuauggaacg gucuggucga aaaucuuuug 2040
caaucucacc ucuaucugcg gccacuuugg aagcuuuaaa accgucuuua uggaacacuu 2100
uucaacguga uauugcuuug gccauggugu auacaucauc aauucaacgg acuacuacua 2160
gagggguuga gacaaccaug cacaccucgg cacaacuuca agaaucacuu ucuaacacua 2220
gguaguugcc cuuauggucu aagacuacuc ccaaugcuac uacuuauaug ugaccuucug 2280
uagcuuuauu guaauccccu gguuuaaguu uuucauucgu uucauuuaac ccgacgucgg 2340
acccuucuuc gacgucgaug aauacaucuu uuucuccuau guaugaggaa cugguaguua 2400
ugcgauucac cgcgacaauu cuuauaauaa gucaagaacc cuaaugucgg acgccuuucc 2460
ugacugucuc auggccuccc auuuagaugc guguguaaug aagaacgacc acauaagucc 2520
ccuccauaac uguaugauca uucucgcuuu gaucgaaacc cgcuuacaca augcuacguu 2580
gauugucagu ccagcggucu aggacugcaa cgacucgaau auugaaguug acauccaauu 2640
<210> 217
<211> 651
<212> RNA
<213> 玉米根萤叶甲属
<400> 217
uaccgccguu ugucuugacc uggacgaguc ucugguuuac cgcgaugggu uccuuucuau 60
acagucaagu uugaccagga ugauccgcuu ucacggcagc cauucagcuc aaaccaugac 120
uccaagcagu uuccugucaa ggugcuuaug guccucucau gguauccucg ucgaaaggaa 180
uguguuuggu auacggagcu gcuauguuga caauuuaaac uuuaaacccu gugucgccca 240
guucuuucca uggugucaaa ucgaggauac augauauccc cgcguguccg ucgauaucag 300
cagaugcugu auugguuagu ucuguguaag ccgucccgcu uuugcaccca cuuccuugaa 360
guuuccgucc ggucaggcug cuagcacuau cgaaaccggc cguuguucgu ucuaaaccgg 420
uuguuugcau accaucuuau gcuucuccgc gucugcauac gacugcuuuu gccgaaugaa 480
aaauaccuuu gaaggcguuu cugccguuac uugcaguugc uauauaaaaa ucguuaucga 540
uucuuugacg gguucuuacu uguuuggugu ccaguuccgc cgucacgggu uccguccgcc 600
gaucgccucc cgcuaagccc gcgguuccgu gggccuuuaa caacguucac u 651
<210> 218
<211> 279
<212> RNA
<213> 玉米根萤叶甲属
<400> 218
uacuucaaaa auucuagcug ucacacgaug uaacgguaga accguuaaga gaaaugggag 60
acacggcuac uccaacuucc uuccucuuuu uaaaacuacc ccgcuuuuuc guaauggucc 120
uguauagaag caccuuuacg acaaggacgc auacacuauu auuaggaaca uccuuaacca 180
guuuaguagg acccucccua uaacaugcaa cguaacuccu ucuucuagua acgacgugga 240
cauugccgua guauacguca ccgaucuguu cuugguauu 279
<210> 219
<211> 474
<212> RNA
<213> 玉米根萤叶甲属
<400> 219
uacgucuaga agcaauuuug gaauugccca uucugguagu gagaacucca gcucgggagu 60
cuaugauagc uuuuacacuu ucgauuuuag guccuauuuc uuccuuaagg gggucugguc 120
guugcagagu agaagcgacc uuuuguugag cuucuaccag cauggaacag acugauauua 180
uaaguuuuuc uuaguuggga agugaaccac aacucuaacu cuccuccacg auucuuugca 240
uucuucuucu uaaugaggug gggguucuuu uaguucgugu ucuucuucuu ccaauucaau 300
cgacauaacu uuaaaauauu ccaacugcuu uuaccauuuu agguggcuaa cucugcacuu 360
acggggcgac uuguuacacc ucgaccacag aaguaccguc gguaccuucu guccguaaug 420
acaccguuca cgccaaugug agaacagaag agguuugguc cucuacucuu uauc 474
<210> 220
<211> 747
<212> RNA
<213> 玉米根萤叶甲属
<400> 220
uacuacaggu uucgucugug uguccuucua cggaggaagc gguuuaaccu uuuagucuaa 60
cgauaguagu uuaugcauua ugagaaaugg uugcaaaacg uuacccgaga gccacgucgu 120
uagaagcgag aaacggaaac cgaugcuaag cuccucccgu aaguucuuac cgaggucuuu 180
aaccuaaguc uuguuaaaau guagccucau auacaugaau aucagcgaag ugacuagcag 240
uacuaacaca ggaaauaucc uacauaauca cgggacgucc ucucauggua ccgggaaaau 300
cacauguagc cguggguuca cgagucaaaa uauaagccaa auaggccaag ccgccaagaa 360
gaccuauugu cgcggucucu aagggugaag guuggcuccu aggcucucuc auacgcugca 420
gaauaguacu uacgaguagu gcugguuagg ucuguuugug aucgguacua aguccuuuua 480
caaccaacga cgccucgacu accgcguugu cugauggaga gagaagucgu cggggaaggu 540
ucagucacgu cucuguggca augaccuuug gguaagaagg ugccuacaca ucuacuugag 600
uggaccaaga agcuucuuuu uacaccaacc uaucguccaa aucgauaccg cuauacguac 660
uaauugcagg aaucauaaca aaauagaugc caugaguagg uccguaacuu uuuucuucuu 720
cuucguaggc uaaguauguc cucuauc 747
<210> 221
<211> 375
<212> RNA
<213> 玉米根萤叶甲属
<400> 221
uacagaccug caccguuccc uccguuucau uucccuuuuc guuucagggc uaguuuagca 60
cgaccaaaug uuaaaggaca uccagcauaa guagcaaaua acucuuuucc uuuaauacgg 120
cuuucucaac cacgaccucg aggacauaug aaccgucgac aauaccuuau aaaucgacga 180
cuucaaaacc uuaaccgucc uuuacgucga ucucuauugu uuuucugggc auauuaagga 240
ucuguaaaug uuaaccggua uucuuuacug cuccuuaacu uguuuaauga caguccucaa 300
ugguagcggg uuccaccuca uaacggauua uauguucguc augacaaugg auuuuuuuga 360
cuuuucuuuc gaauu 375
<210> 222
<211> 864
<212> RNA
<213> 玉米根萤叶甲属
<400> 222
uacuucaacc uaccacaucu agacgggggu gguuaaucga agcuguaacg ccuucucguu 60
ggcaauggug gaacgguugu cugcaagaau acauuaccac uaccuccuag guaucacgcu 120
gucaaagagc ucgacauaaa gcauuauaua cuaagucuau uauccgucag ggaagaaguc 180
cguauagugc uuuuucggug uaaaaguuac uguuaccgga ugggcaugcc gauaagguuu 240
cugucauuuc cucauagcac caacuuaacc auacgguggc uauuaucuuu aaauaaugcu 300
caaguucuag gucugucuuc uuuguucaac aauucugucc cuguucaacg acaucaaagc 360
aagaacguuc uauacggcgu gugcuucgug cuauaagugu caaaauguca ucuaaacugu 420
caaaaaugug gggucuacaa uacaaacugu caccgaccau acaaauuucu uaacuuuuca 480
ccgguguuuc auggaggaaa uucuauaaag aagucuuggg aacauuaaca uggacgaccu 540
aguccaaaaa cguaucguuu acuucuugaa guguauaggu uacguugagg ccugguucgu 600
uuucuacgaa aguucuggug gcaauuacau cgaggccgug gucggggaca cuaauggaga 660
ggaccugggu cauauggugu ugggcgacac ggucuacuac gauguuuugu ucuuuaccau 720
uuugucuaca ggcgucauag gccuuacuua gagcucacca gcgaugucac agagcuucuu 780
uguguuaccc ugauggucuu ucgguauuac cauaagguuu uaaauuugcg uguuccacaa 840
caugguguuc gucguaaaua auuu 864
<210> 223
<211> 2868
<212> RNA
<213> 玉米根萤叶甲属
<400> 223
uacugacgcc aucuuguugg aacaaugugu gauuauuuga acgguugucu aagccucggg 60
auguuacuuu acguugauuu uuaccuaaau cuuuucccac uccaauuuca uuuuuauucu 120
cguaaucuuu uuuauuaagu guacuaagac cguccucuuu ccaacggcuu accuaaagau 180
uacugguagu auucuuugca aaauggaaau guucuaguaa accguuuuuu ugauaauaac 240
uaaaagaccc uuuaucaagg uuuuuguuua ggucuuccau uugaugaugu ucucuacuaa 300
aaccauacac uacggauauc uuuucuagac guuguggguu uacuuaaaaa cucuccaaga 360
ugugaagcga agaacacguu ugacuuccuu ggucuuaaca accuugguaa uuacggguca 420
uaaucucgaa caaaccuagu auccgugucg auacacuccu ccuuacgaca ugaccguuaa 480
aaaugguaaa uguuuuuaaa acuucgggag uaaggucuac gaggacuuga cuagagguua 540
auaaaccuac cacucguucu guacagaaca uuuucuuuac gcaaaaauua cgaagaagua 600
cgacugguuc uuucccgcaa cagcauaaac cguaguacaa aucuaguuca uuuaaguaaa 660
ccucuauaag auguugacca guagcaacuc aacuauauau uccacacagu aagguuagga 720
cgccuuucua gaucuaaaua aucuacauau auauugaaca acuugaguuc gucaggacga 780
caguccaugc uucgacgucc uugaaaucag ugggagaggu cacggggcug acggcaauuu 840
cgacgacgau cgacaaugua acucaauuaa uaguuucuuu cacuguuguu acauuuugag 900
uagcaaaacc uguccgacua ucgugaauuc cucgaaggau uagugcuuuc uuaagacguc 960
cuaaaucaau accuguauga cucucaugag agacgaggac ugaaucuuca ggcguucuuc 1020
ugaaauucag aucgggaacu uaaucagaga agugccuugu aucuucuuua ccauaaucau 1080
aauuguuucc uucacucauu uugccaucug ucacuuguac uccuaugucc uuucaugucc 1140
guuaacaauc auuccugaga uguaagcaca agguaauuca agggucuaua gcgugcauca 1200
caauaugguc agaacuaacu uaaaaauagg cuauuauuac uugaccgacg gugucuacau 1260
aacgacaaga auucccuucg guaugucuuc aaauuucuua acguuggcaa uuaauaacuc 1320
uuugaguagc uuugaaaguu ucuguaauuu aaccaguuuc agguaucucg ucguuaaacc 1380
uaaaacccuc uuaugcgcuc augacgaagg cuauaucuuc aauagcaacc ucuuuaauug 1440
ucuaacaacc cacuuccuag ggagcaacuu cgacucgucu ucaauuaucg uccucuaugc 1500
cuucucuuac gaggacgugg acgacguccg cgguggugaa aucaaugaag gcuaccuugu 1560
auacgauggg uuagucgaaa guugugacag ucgguuuggu gauuucuucg ugcuggagga 1620
gauucuguua uggaguaccu accacuaaaa aaguagccuc ggagaaaccg uagauguaau 1680
ugguuugaca gaaacgccau acuccuggag uggagaggac gacgaucguu accuaaguua 1740
cgguuuuaau acgaauaaua ccgaccuuaa gaagugaacc cuuuuagucc ugaagggugu 1800
uuuaguuauu gguugcugcu auuucuggug uaagacaaga caaaugcuca ggauagacua 1860
gcaagagguu aguaacaacu uuaaaaguuu uuuaacacgg cgagccguga uuuacucuac 1920
gaagaucgau uccuuagcca ucuucgcuag agcguuuucu cguuucuuuu uuuguucgca 1980
ugcuaaguuu gacugcugcg auauucgaag gacguuaauc ucugucuauu uucaccucuc 2040
gauccucuuu ugcauaagcu cuacagcgac aguguucgaa aucauccucc agcuugcccu 2100
ccaccgcuua gucauaauuc aagguuauuu aaucuauuuu aguguguuga cugaccaaaa 2160
aggcuagguc aaauaaggcu ucguaugcaa gugcacuuag ucaugcuaua gcacgaacua 2220
cagaauuagc auuugguuug auugcuauga aauguuuuga cgugcgaucu cgaccgauga 2280
aauccgcuaa acuucaacca ucucuucggu guuggacagc auaaccgcgg guuucugaaa 2340
acguuguaau uucgauugca cuuucaccgg aguugacuuu ugccuuaaua uaaaccguug 2400
uaacacauac uacaguaucc ucgccccagu cuauccuuac aacaucaaaa cuuacuauau 2460
guguaucuau auuaccugau auaucacgga cgaucaacau gucuaucgcu caaauacucu 2520
uacacccgcc uuaaacuuac ccuuuuauuc cauuggcaau ugugugggga gugccuugaa 2580
agucuuaugg agcuuguaga ugaguuuucg uguuuaaacu uuacaaauug uagucuuuuu 2640
cgagacucgc ccgucacacc aaaauaccgu cgguuaaaua uacguuuuag guaaaaaccu 2700
cuucugcgaa accgguugaa uucauaucuc uuuggaaaau uauuugggcu acgcggucau 2760
ucgccaguau aaucuuauuc ccgguuuuca gucccguacc ggaauucaaa uccucuguuu 2820
caguuauacu gugucuucuc guguguugua uuucaucauc gacguauu 2868
<210> 224
<211> 549
<212> RNA
<213> 玉米根萤叶甲属
<400> 224
uacccauuac acaaacguuu aaauaaguuu ccggagaaac cguuuuuccu uuacuccuau 60
aacuaccauc cugagcuacg ucgaccauuu ugguguuaaa auauauuuga auuuaauccu 120
cuuuaacauu guugauaagg uuguuaaccu aaauuacacc ucugacaucu uauauucuug 180
uaaucaaaau gucauacccu acauccacca guucuauuuu aauccgguaa caccucugug 240
auaaagguuu uguguguucc ggauuaaaag caucaucugu cauugcuguc ccuugcauag 300
ugacuccgau uucuacuuaa uuacgcauac aaccggcuuc uacuugaauc ucuacggcau 360
gaagaguaaa agcgguuguu uguucuaaac ggguuacguu acuugcgacg ucuuuagugg 420
cuguuugagc cagagguaag ugaugcguug gcguugacca uguaaguucg auggacacgu 480
ugaucgccuc uaccagagau acuuccagac cugaccaaca gguuaguuaa uuucuugcgg 540
uuagcgauc 549
<210> 225
<211> 4425
<212> RNA
<213> 玉米根萤叶甲属
<400> 225
uacccugcca acgugacaaa uaaaacauaa aaggauacaa aauggcagua guugugcguc 60
uguugcuuau auguaccuaa gagccuuuua aggcaccuau guaaagauag uggaguaccc 120
uuuucacguu uuaagcacgu gguuuuagug uuuggguuuu aacuuuuaac agucuugaug 180
uuugggagcc acuuucuucu ugucgguccg ccuugcaugc auuguugcca auggcgauag 240
cuacugcuag gaucccuccc uccuccuugu uaaucaaugu uugauuaagu aucucuuccu 300
cuuguacaaa auaaacugua ucuguugcaa ugaccacaaa acuguugaua ggucgguaaa 360
cuagcccuac uuggucauuc cgucuuccuu gaaauacauu ggcauguucg aagucuguug 420
ccguccggug guaaucgucu acagacaugu aaaugucaau gguaauggcu guaauuacua 480
uuacgcggug ucgaacuauu ugacuuuaug cuacguguuc aaagacuucu aaauuuucau 540
ccuucacuuc acuacucuca aaaacgaaug cuguaacuac uaccccuuuu aaguucuaau 600
agcauauuga aaaguuugcu uuuacgaguu aaguggguca uaaaguccua ucuagcucua 660
ugaccgcaac acauaaauuu ccuucgaaau ugucuguuuu ucuuaugauc uaaauuguca 720
cgacaaauac aucggcuauu accgcaauug cuaguucuuc cgguuuuucu aaguuggcga 780
uucuauagau auugucauca ucccagacua uuugucggag ggucuaaaug aguuuuuuac 840
ggacuaccuu agaaccucua ggggcuucua aaauuucuga aaagauuugu auaacagugu 900
cagcuucguu gcagguugua acgccuaggu guugaacgaa aacuuaacca cuucccuuuc 960
uguauaguuu gguuauuucu gguuugcaaa gaaaaccucc gucuuccuuu auuucgcgug 1020
uauuucgauc acgcagguga ccuaauacuu ugucauugcc uuauaugaga uugauaagcu 1080
cauuuuuugu uucuaaauua ccuaagaagg uauuuauaug guuaauuuca uaaucuacaa 1140
uuacuacuuu aaggauuaaa ggaacuuaaa gaauuuccau cacagcaccu uuuacuguuc 1200
gguccacgug uucgcuaagu ucauucucgu uagcuauuuc ugccuugacg acgauuguug 1260
uaacacucga uacuugagca acuguuaugu cuaaacaaac guuauuuggc uagaugcccu 1320
cauuaaugca gcucucaccu caaacuagca cuuugacaug gagauauagu gcauuugcaa 1380
uuucgaauac uauugagagg cagacgaaac auauugugcu guaacggauu guaacauuaa 1440
gucuguaagg uuuagucaua ucuucuaguu uuacuguugu uuggacauaa augaguaggu 1500
uaaauaguca agucauuaua augacucgaa cgacuauuua gcucauaaca accacuucag 1560
uuucgaaauc uauuacugug ccgaagucaa uauucaauau cauaauguuu accuuuauaa 1620
cugcuacgca aauacuaacu uuuaagaugg ccgucuuauu cucaauuacc uuuugaccua 1680
augcucuuuu agcuuguuau guugaauugg caagcgcgua aacuaccccg uaaacuucua 1740
aaacguuaac aaaauuaaag guaugaauua cuuuuacugc uuggagguca aaaacugcug 1800
auauagucuc uuuaaguuua auuucuccuu cuuggauacu auaggccuac gcaacaaucu 1860
cacugacgag uacuaggucu auaauuucug uccguacgac uaguugugua ucauauacuc 1920
cagcgcuuuc uugucuuucu aaaaaacugg cauagacggc uaccuacgca uguucauugu 1980
uuuggagagc uggcucuagg cggaaagcca ucggguugug cuguucagaa guagauacga 2040
gcacuauuac uaccuccgug uuuaaguaac aaccggugac gucuuuaacu uuaauaaaau 2100
uaucuauauu ugcuauuacg agggaaaaau uuacaauguc uuuaacaaau aauacuuuug 2160
guccuagguc caaaauaucc auuggauuca cggcuacuaa ugcuaccagg acuauuaccu 2220
ggaggcaaac gaaaagcuaa uagucuguga cgaagucuau cauaaucuag cuuuaaaagg 2280
uaauagccuu uggucgaaaa gcgaaaucuu uacaaacuau cucuucucgu uuuuauaaua 2340
cuguaacggu aacuguaaug ucuaucaccu cauggaggug auuguccuug aucauaagaa 2400
ucucaauauu agccucuaca uuuacuauua ggucgauguc ugccuuuguc gugcuagaaa 2460
cacauauuca ugcaguuacc cggucuuuua aaguaccuuu agccugcaca uauacaaugu 2520
cuggaucugc uaacccuaaa uuuacuguuu cagaaacaag uucuucuauu gaaacuacuu 2580
aaacacaauu uggucguauu guugccauac uaagacuacu uugguuguug ccgacucccu 2640
ugaauacucc aaguaauguc ccagugacuu uggguacuug gguguuaugu gcuuguaugu 2700
caauuacguu aucagugcua augucaauuu caugaagguc uccuucgcca acauuuuagu 2760
ccuaguuaag cuaacucucc uuguugauuc cuucuuaagu aucuuuuaag uaacuuaccu 2820
uucucguuuu cucuguauaa ugugguucuu gagagguuuu auaauuuaug uagaaaucgc 2880
uuacaacuac auaaaugaca aaauuuaagu gggguggucu uaucaagcaa acaccuacaa 2940
gcuaaaagac gaguaccuag agguauaaua cgaggucucu uugagcuuuu guuucaaugu 3000
cuaguaguuu accucgaacu uguuuuuaau cuacaccuua agauguacua guugcauuug 3060
cucacggaau ugcuuuguug cacaccucga cuuuugagua caugcuuguu uaauuuguau 3120
ugugcucuug gucgacauca caaaugauug ucuuguagga aacagccaca uuuacguaaa 3180
uaacuaggac acacacggcg aaaugguucu cuacaauacc uuacaaaguu gccuccgcag 3240
gaauagcuuu ugugucgcac auuaacagga cguccuaaac uuccuggugu aacacuuuag 3300
gaucgauauc cuaaaugucc uugaccaacc cgauacauag guaggaaacu gcgauguuug 3360
uccugacucu aauaugacgu auaaaauagu guuugacuau uaccaaacua uaaaauguua 3420
ccuggaaauu uauauucugu uugaagaaac agauuucuaa uauauaguaa ucuugaauuu 3480
cugccuauag guaaugaagu uuaaacgugg ccgaguucgu gaguucuuua aauagacuuu 3540
cucgcguaag uguuuaacuc gcuaccuagc aauguguuuu auuuuuaucc uagaccuaaa 3600
cugcuauaua gggaccuuca ucugcugaca ccuuguugca caaguuaaac cugauuauuu 3660
gauguauuuc cacaauaggc ucguuuaccg ggggaaguug acccuccaua cuuuuugucu 3720
aaguggcuag uucuuaaguu ugcuuaaacc cugguaaacg guggcugacg gugggcaaag 3780
agaccaacau aaucuuuaaa cugcauauua cuuaaaauga uguuggagcc acguggaaga 3840
cuacguaagg uucgcauagg gcugacauug auacgucacu acguucgaca cugaaagcca 3900
uagcugaggu uaaagaacca acgauaagac caaacacauc guuaaaacua uuaagaagaa 3960
gaccgucauc aacauguauc ugcauuugug cuguugaaau ugcuuuuucu uuagcuacua 4020
ugagcgcuuu uguaauaguu gaugcuucua cuuccaccgc cgcuuacacu gugguugaug 4080
cuggacagac aaaagguagu cuuguuguaa caccugcuuu uugguaacua cucucuguug 4140
gggcuacaug gacgucuaua uucaccgaaa aaucuauugu ucuuucuguu gacacuauuu 4200
cuggggcuau uaaacggaau acugcugcaa gcgguaauac ggaugcuccc ucugccuuua 4260
ucguggccua ggaauagaag agagcgaagu acaugccugc uuccucuaaa uuucaaguug 4320
augaauaguu caaaaccugg gucuaagucu uucaaucggc uguacauacc ucuucuaggu 4380
ucgcuacuuc ugagugugcu accuuugcuu cuuaggacca cgauc 4425
<210> 226
<211> 291
<212> RNA
<213> 玉米根萤叶甲属
<400> 226
uacggaaaga caccaggguu uaacagggag acgccggacu aauagucacg uaccccauag 60
uaggucaacc caaaguaccc acauaagaua auguaacccc gacaccgaaa ucgucuucua 120
uaaggucucc aacucaaauu cccgcuaaau cuguuuaaaa uaucgcugca guugugccca 180
aagugugucu uacgaauguu gacgaccuaa cgacgagagg auauggacua uuguaaucgu 240
cauagucgag ugguuaagac ccgguuguug ucuaguagua acuugcagau u 291
<210> 227
<211> 540
<212> RNA
<213> 玉米根萤叶甲属
<400> 227
uacccagaau gguauagucg ucacaaauua uccaacaaau cauuuuucgg auacucuuaa 60
aauuaccauc cuaaucuacg gcguccauuu ugguguuaga auauguuuaa cuucgaacca 120
cuuuagcauu gauguuaugg uugguagccg aaguuacauc uuuggcaacu cauguucuua 180
uauagaaagu gccauacccu acauccaccg gucugcucuu agucuuuuga gaccucugug 240
auaaagcggu ugugacuacc ugaguaaaaa caccaacuaa gguugcuggc ucuggcauag 300
cgccuucggc uucuucuuaa cguguuauac aauccucucc ugcuaaauuc ucugacguaa 360
aacaauuaua agcgguuguu uguucuaaau ggcuugagcu acaggugacg acuuaacugg 420
cuauucgaau ucaacgugug aaacuucuua uccuccacca uguauguucg guguacacga 480
ugaguucccu uaccaaacau gcuuccugau cuaaccaaca gcuuacuuaa ccgguucacu 540
<210> 228
<211> 618
<212> RNA
<213> 玉米根萤叶甲属
<400> 228
uacgccaugu gaaacucaau guagccacga ugggaucgca ggugacauug ugacuaaaaa 60
cgggagauga ugacggagug cccuuuuccu cucguucaau caaaucguac caauaacaac 120
uuacacagag ggguguacac ccguccagau ccuuaaccgg aacgacauag uaauagucaa 180
cauccucgac gacguccuua agugugaugu ccucagucau agcauccucg accacaauuu 240
cgggggucuu aguuuugguu uuuaaauuaa agauaauaaa agacacuucg acaccgauag 300
auacccaauu aauaccgaua ucaugagaca ccuucaaccu ucuuaaagcu acaucuggau 360
aaguuggagu uuugaguauu gaaacgaguu uugguaauac cuaguguaca auaaaaaccu 420
aggccaaauu gacaaccuaa acauuuagau aauacaccua aaacacaacc ucaucaacca 480
agaccacguc gguaaagacu acgucgguua aguaguaaua agcaguuuua aaacuaauaa 540
cucuaaaaac cuucacggua accagagaag ccagacuaac aaccucauau gaacugcagu 600
ucuccgagau accaaauu 618
<210> 229
<211> 5694
<212> RNA
<213> 玉米根萤叶甲属
<400> 229
uaccgauggu ugcuaucauu ucgaggcaac uccugucaau uuucucacgu uaaaccuuau 60
gaaucagguc uacuuuaauc ugcuuacagu cagugucuuc ccccguaggc gaagggucuu 120
ugguaccuuc guccggcggg guuugauacg ccagaauacc uggggucugu uccacaguau 180
cugucuucga guucuacggu cuguacacgg ccuuuauacu gucuuacagg accuguaaag 240
ccuguauagc ucgaccguuu uggucaaaag gugcauccua agcauuguuu uuguuauuuc 300
uagaacucua cgcaaacgaa gaaaacguca uuuaauaauc agucagguuu auuaggcuuu 360
uaauuucuuc aacauuacuu uaguuucccu gucggugcau uuucuaaucg aaagcaaaua 420
cuagacacau uuccauuuuu auaaacacuu ccaccucuac uuuaccuaca cccauuucuu 480
ucgcuucuag gguuauuuuu ucguccggua ccaccaacac cagcuauagu ugguuuauag 540
ucugcacggc caaaucuaaa uugucgucuu accuuugugc aguuacuucu guguguucuu 600
uucuuuuagc gugauagacg gcuugcacag acccuuuagg auuuuguaua gugucuacuu 660
cuuacaaagu aagaaccaua ccuaggguuu aaacgaucug gucuaaccua cuaucauugc 720
caugaaggac aaggagggga ucgucaugcu ggacgacauc aauacgugcc uagacguucc 780
uuaguccuac uauagugagu guuuaaccgg cuguaauagu uccgcuuauu gcuuaauguc 840
uucuugcuca gacguccacg ucggcgagua uauuaguguc uuuuauaauu cuacaacguu 900
aaagugcagc ggugaaauca acuguugcua uacggcccuu acggcucucg uuacguuuuu 960
agaccuuuug gggauuuucg auaguuucga gccgacuuuc cauuucuucc uuccuaagcu 1020
ccauuggaau acccuuucgc acaccugaaa agacgugcau gacaguagug uggucuaggg 1080
uuaaaugcau agcugguuca uccucacgga ucuucauaac gaguuuugua cugcaaaggu 1140
cuuuagcagu guggaaaguu aaaacuguuu uacaaccuua accaugucuc uccauuaaga 1200
gucauagguc cucgauucau auaguagucu cuguuaccuc ucuccuaacu aaaugcaaag 1260
guggguuuug gcagucuaaa uguaaacguc acaccaauau uccaucuuuc uguguagucu 1320
cugccgcuag aucauuagaa guuggcaguu gguugggagg uguucuacuc auacuacccg 1380
gugucucagu uucagaaugg gaccagcugc aaggcauacu uagagagcac guggagaggg 1440
auguugcggc uaaaacugcc gcugcuuuac uuggagguac acgggguuuc auaccuuuga 1500
gcucgacuuc agcuuuugga gguguaguga ggguccguuu aguaaugagg cguucgauug 1560
guugggcagu acccauaaca uguucuaugc aacugucgac aauccuucua cuguuuuucc 1620
cuacauaagu agcucuuccu uguuuacuac uuauauaacu acaagaacgg uuaaacccua 1680
ccauuuuacg gggcaggucg guaggaguuu ggguuuggca acaccugucc uuuugucuau 1740
aaaagggacu aguaaggacc guuacauuua uacuaugcau ggguaagaug cguaggucug 1800
cugcuccugc ugccagggau auuuaccuau agcgguccuc uaugcuuuca auaccaucuu 1860
guaccucuua accaguaccc auauaacaca uucuuuucag aaccuuguag ucguccaagg 1920
gacgacguau aaacauacaa ccuuaauccu gugcuucaca caccaucuaa aauaccauug 1980
uaaguuugac auuaguuguu gaccaacaac aaucuuccag ugucguagcc auaaccucug 2040
ugguaacggc uaggagucug aaugugucuu uaagucucuc gguaguccuu ucgguuucuu 2100
cuacauuauc uucaguaggu cuuucgagug uuguaccuug accuuggcug agggccauua 2160
ugcaacgcag ucugaaagcu uuuaguucau uugucuuaag auuugcugcg agcacuguuu 2220
ugaccaccaa ggcgauucuu uagaaacuga cuuauguuau uggauuuccg auaccagcau 2280
agcccuaggu ucccuagguu guaauuauaa aggguccaau aacgaacgca cccaguuguc 2340
uugcaucuuc cauuugcaua agguaaaccg aagucuuuug cgugcaacgg cgugaaguag 2400
uuccugcuaa ugccaggacu uaggucucca aagcaucuuu uaagcauaga acggccagag 2460
ugaggaagcc ucaagauaaa ggugcgauac ccuccagcac uuccagaaua gcuaugacga 2520
cauuuuugac ggcuuugacc aauguagguc gcagcagacu aguuccgaua ccucucacau 2580
uaccauguga ugcugccaug gcauucuuua agacauccug uugaauaggu caacucuaug 2640
ccacuccugc cugagacacc ucucuaccau cucaaaguua uaaaucguug ccaguuuaau 2700
ucauuguucc gcaaacucuc uuuuaagucu aaacuaggcu cauuacuuuc cauaaacucu 2760
ucucaaaagu uacuucuuca auaguucguu gacuacccaa guccccuuca guaaaggcuu 2820
gaacucucuc uuacccuugu ugaggucuuu cugucucuuc ggaauucugu uuagaaggga 2880
ucgccucuua gguuucauca ugaggggaca uuaaauguug cauacuagac cuuacauguu 2940
uuuuaaaagg uguauuuguu ugcucggggc ugucuggaca ggggcaauuc ucaauagguu 3000
ccgcaagcuc uuaaugaguc cuuuacgcag uagcaucgac cgcuccuagc agacagguuu 3060
guucgguugc uuuugcguug caaugagaag gucacagauc agucuagcug ggagacgugg 3120
uuuacgcaaa gacuucuuaa guccgagucg uggcuucgga agcucaccaa cuauccucuu 3180
uagcucugcu ccaagguugu ucggguucgg uuaggaccuc uuuaccaccc gcgagaccgg 3240
cgcgucagug acccucuugg gcgaugaguc uacugugacu ugugaaaggu aaaacgacca 3300
cauaggaggu ucuugcauug ggacccacau ggaucuaauu uccuuuaaua auuauaaagg 3360
uucuuugggu uccgagguag agauuggcac aaaaauugac cacgccgacg aucucuacgc 3420
cuuuuucgcu ucuuacacaa uacgucugaa cuuguguggu gagaagcauu ucauuggcgg 3480
uuguggcggu agauaaugcu aggacugggu guuuuauggc aguaaggacu ccuaguccuc 3540
aagcaauugc agaugauacu uuacgggcua aagcuaggau gggcauauag cggcaccaac 3600
gaagcauagc uugaccuguc uuucucuuac ugucuauucu uugauugaua ccuuguuuaa 3660
cgacuuuucu aguugcgacc caagccccug cuaaacuuaa cauaaaaguu gcugcuguua 3720
cgacuuuuca accacgacgc auagucuuag uacuugucgc ugcuaccuuu uaagccucuu 3780
ccacgacuac uccugcaucu guuuuaccua cugcuguaca aaaacucuac guagcuucgc 3840
uuguacgacu cgcuauacug gaacguucca uaucuucgcu aaagguucca uauguacgug 3900
aacggugucu gacugagcuu uuuuuccuag caguagugac uuuguccgcu uaaauuccgg 3960
uagcgucuua ccgauaaccu uugacugcca uggucguacu acuuucauga cagucuuucu 4020
cugcagcuag gccaguccug caaaagauug cuguaaacac uuuauaaaag ccaugaacca 4080
uagcuccgac acgcauucag acaucucuuc cuuuacuugc gacaggaaag caagaugcca 4140
gacauacauu ugauagcggu agaacggaac gaaacacugc auuacugucg guuuccagug 4200
aauuaccggu agugggcagu gccauaguug ucuguucugu gaccucgaga cuacuccaca 4260
aggaagcucc uuugacaucu acauaacuac cugcgacggu caguacgccu ccagcugggu 4320
uacucuccuc auagacuuuu guaauaggag ccaguugaug guucuuaccc guguccgacg 4380
aagcuagaaa acgaccugcg gcuuuuuaca uuuuacccuu aacgguaugg aguucgcgug 4440
ucgucgcuag auuaccgaag uccuuacaag aaaccuaauc ggcgaugugg gucgucauac 4500
ucagguccac cacgauacug ggguaccuua guucgucgau gugguaugca accgucauag 4560
accagaggug ucuuaaauua cccgucaccu uacugugguc caccacggcg aaagaggggu 4620
agucgacgca gucuacguag uccuuacagu ggucgaauac cgccaaccag ugguuguggu 4680
guuagaggac guuacagcgg uauauaccga agagguguac cuguuagcgg aaggauguca 4740
gguaguucag gucgcaaggu uggaugaagu gguagguacu gcggcuggag aggaccuaua 4800
agagggucaa gaggaccaau aaguggaugg ucagaguuaa ugucagguug cucaggguca 4860
auaagugggu gaagagucuc aaugaggggu uggaguggau caaugagugg cugaagaggu 4920
uuaauaagug gaugaagggg uucgauguca gguuguaggg gauugauaag ugguuguaga 4980
ggguugauaa gugggugaag uggaucaaua ggaaguugaa gcgguccaau gucgggguga 5040
agugcgucga ugagugggug uagaggauca augaguccuu gaagcgggag aauaaguggu 5100
ugaagcgguu caaugagggg augaagagga ucaauaagcg gcagcagagg auuaaugaga 5160
gggugaagag guuuaauguc agggugaaga ggauuaauga guggcaggag aggauccaug 5220
ugcgggccaa gaggaucaaa aagggguuca agcuugucaa ugagagggug uagaggaguu 5280
auaagagguu guagagguuc aauaagcgga agaagcgggu uuauaagugg uugaaggggg 5340
uuaauaagcg guuguagagg uaguaaaaga ccuccuucag guguuauaag uggguguagu 5400
ggcuuuauga gagguuggag cggguuaaug ugagacagcu caggcgucgu gugagguugu 5460
ccaucgucag cuauaagugg caguugaagc ucaauaagag gauuaagcgg guuaauaagu 5520
ggcugcagag guguuaugag guagguguca uguuuuauaa ggggacguuc aggauguaag 5580
uguggguggu caggaucaaa gagagggcga agugggcgua uaagcggagu uggauacaua 5640
aguggaagaa gaggauuaau aagaggguga ucagggucag uucugugacu gauu 5694
<210> 230
<211> 876
<212> RNA
<213> 玉米根萤叶甲属
<400> 230
uacagcaguu uauaaguuuu ccgggucguc aacuaccgcc uacgucuuuu cuuucauugu 60
agagcuccaa agaagccuag agauaaaccc ccuaguucag cauaacuucu acgucaccuu 120
acaauguguu cucgacguuu ggaaaaauuu uaccgguucu cgacccuacg acggccauuu 180
cggaaaacac uccgacgauu aaacguaagg ucuugaccac gagcaguacu gcgacgguga 240
uuaauauauc uacgacguuu aacaauguuu uuucggcuac auaaacuccg acauuugacg 300
aaauauuuuc gauaucugua aauauggcuu uacccagcga aauguuaccg acguuuugug 360
guagucugau aacgucuuua cauacucuga cuacgacacc uguagcuuuc ccgacaaguu 420
gugauacuug uccgccgacu aaugaagucu ccucuucuuu cguuacgaag gcgguuauuc 480
acagaagaau uucaccgagu uauacgucgg guugaacuuu ugauacuuuu ucgucacccu 540
uaaauaguuc uucaccgaau acgucgagac cuuucgagag aaaauuuuau gucacguuuc 600
cuuaugaaua agucucgacg ggaaacagug gaaacacaac uacaugaguu acguguugua 660
cgauaucuuu cgauauaaag uuccauaggg cguaaaguuc uaagggcacu uauguuugaa 720
aacuuuuggg aguaucuuuu guagcuucuc guuuugcauc uaccuauaug ucuucggcag 780
uuucuaaugc uaaguuaaag agcagaacua gucaccauau gauguuaaga aaaugcauaa 840
uucuuuguuc auucgcuuuc gggacugaau gcaauu 876
<210> 231
<211> 609
<212> RNA
<213> 玉米根萤叶甲属
<400> 231
uacuuagggc ucauacuaau aaauaaguuu gaagacgacu aaccucuaag uccucauccu 60
uuuagaacag aagaugacuc uaaacgucua cuauggaugu gucuuucgau auaaucaugg 120
uaaccgcauc uaaaauuuua guccuguuag cuaaaucuac cuuucuguua auuuaacguu 180
uaaacccuau gucguccagu ccuuuccaaa ucuugcuaau guaguucaau aauggcuccu 240
cguguaccau aauaacauca caugcuaacg ugucugguuc uaaguaaguu auugcaauuu 300
gucaccgagc uucuuuagcu ggcaauacgc acacuguuac auuuguuuaa ugaccauccc 360
uuauuuucgc uaaacuguug auucuuucaa cagcugaagu gaugucgguu ccucauacgg 420
cugguuaacc cauaugguaa aaaccuuugg agucgauucu uacguugguu acaucuuguc 480
cggaaauacu gauaccggcg acuuuauuuu uuaucucauc cuggagguag aagacgccau 540
cugguuccuu uauuccaauc caagcuaguu ucagcggguc agcuuuguug guuuaggcca 600
acgacgacu 609
<210> 232
<211> 789
<212> RNA
<213> 玉米根萤叶甲属
<400> 232
uaccgucugc gacuacuaga uaaucuaaua cuucuacucc uugucugucu uguuuggcgu 60
ugacuuugcc guugaugucu ccaugucuuu uucccacagu ucccguguau acauaguuau 120
gugucaagac ccaaaucucu aaaagacaau uuuggucguu aagagucucg auaucaccug 180
acgcccaagc uuguaggaag ucuucaaguu guacuuacau aaggaguucg acaguaaccg 240
uaccuauaag acacgguucg auuuaggcca uacccuuuuu gccgacaaaa acauaaucga 300
ugugagguuc auuaucuagg augucuuuua caacauauac aagagcagua cacgguaugg 360
ucucucaauc ggaaggucua uucguuucuc augcuugcaa agucauuuau auacggguua 420
uaauuucauc cccagaagaa accaccgaac ggauaggucu uucuacuccu uugcaauuuu 480
uuauuaacgg gcguauagca acacccauga gguccuucuu aaaaucguaa ccagucuagc 540
uuuuuugaau uagaguuugu agauuucgua aaauaaaacc uacuuacacu auuuuacaac 600
cucaauaauc uguacucugc acuacaaguu cuuuauauag cauugugagg ggugcuuuuu 660
guucaguacu acaagucacg guggaauuca uuucuuuaau cuggucaaac guucuuuaaa 720
uacguucuac auuaaguuuu aagaauauua uguguuaaaa cauuacugcg ugggugagcg 780
uuacaaacu 789
<210> 233
<211> 738
<212> RNA
<213> 玉米根萤叶甲属
<400> 233
uacggccagu aacuaccaau auuucaugaa auguaaaaua augugucaaa uauauguuaa 60
aaacuuuuau aaaccuccug agaaaauaaa uaaauaguuu uaacauauuc ccaauauuug 120
ggacuuagau guaagcuacu acgacugguc aauuucuuuu cugacagauc ugauuguuuu 180
uucggaguug uaaauugaua guaauaacca caccuucuua uaaguaacca ucuaaaccga 240
uuggagcaua uaaccacaaa uccagaauua uaaggcaugc aaucaaagau acuaauauuu 300
ccauuaaauu uuuucguacu ucucuucaac guuguuuaac aucuuagguc uuuuagucuc 360
uuauaguugu auuaaaccgu guggguacgu cuuguuuccg uauuuuuacc uaaaaaccca 420
gguuuuuagg ugcauuuuca caauugugug cgccugccuu ucguuucaua ucauuuacaa 480
ugauuuuuua aucgagauuu auuucuuuuu cuguaaucau uucuuuuuua aucacuuaau 540
gauaauuccg ucauacuuaa aggucuaggu cuuuaccgau aauaaacacc uuucuuugac 600
uuguaaauau uaauaggagg aaccgucaau ucugaguguc uuaagaaauu ucaguuguuu 660
caguuguugu aguguaaggg ucacaaacac cuuuuuaacc uuuucauguc guuuacacuu 720
gucucccacc cuuuuauu 738
<210> 234
<211> 480
<212> RNA
<213> 玉米根萤叶甲属
<400> 234
uacugguuga gauuuccaau ggcggcuccu uggucccuag auaaacgggc guucaaauuu 60
uuugcaccac auuaagguga aagguguaua aacucucaga uguuucaacc ucuauaacau 120
cuauaguucc cauuaccacg ucaaguuuuc ccauacgggg uguuucacau gguaccauuc 180
uguccugcac aaaaguuaca augacgugua cguaauccac auuaacauuu guuuucccaa 240
gcuccuucuu aguagggguu uucuuaguua gaggcauaac uuguacauuu ggugagguuc 300
acagcaguuc ugaagaacgu uucucauuuu agguugcuuu ucgaugcauu ucuucgauuu 360
cuuuucuugu aauuucaucu ugaauccucu guuggacggg uuggauccgg ucguguauaa 420
caaucgccuu uccaaggucg uguccacgaa cgaggauagg guauacuuaa guaacgaauc 480
<210> 235
<211> 537
<212> RNA
<213> 玉米根萤叶甲属
<400> 235
uaccuuccuu augaugaccu ugguuguaac auaugguauu uuccauaacg auauaaccug 60
auacuaccau uaucucacga ccgauuuaug augcuauuuc uauauaaagg augucguuuu 120
cucgucuuuc gaaaacucuu uuuaaacaag uuauuuugag uaucccgucu gcgucuuuaa 180
uaguacaacc uaccaaauug aacacacaua ucuucauuac aucuaaauaa gaaaauacaa 240
uacccgucaa guguacuuuu acucgauuaa aauuacucac aaaauuuaac gaacauacug 300
agucauucag uuuauaacuu cuuuuuauac guuuuugcuc gacagaaccu uagugaucua 360
uaacaauacg accgauaccu acuuuaacaa cuaccuccuu auuaauaucu aagacuaagu 420
ucaagucauc auagaucuua ucguaauucc ugacuacuau aagguaaucc ucuuguuuga 480
caucgagucc auaagguuug ccgguuucuu gucgacuuua ccaguaacga cuuuauu 537
<210> 236
<211> 450
<212> RNA
<213> 玉米根萤叶甲属
<400> 236
uaccgacugg uugaguggcu ucuuguuuaa cgacuuaagu uucuucgaaa gagugauaag 60
cuauuucuac cacuaccaug uuaaugcuga uuucuuaauc cuugucauua cucuagagau 120
ccuguuuuag guugucuccg acuuaauguc cuauacuagu uacuucaucu acggcuacca 180
uugccgugcu agcuaaaggg ucuuaaaaau ugcuacuacc gugcauuuua cuuucuaugg 240
cuaucacucc uucuuuaagc acuucguaag gcucacaagc uguuucugcc guuaccaaag 300
uagagucguc gucuuaacgc ggugcaguac ugguugaacc cacuuuuuaa cugucugcuu 360
cuucagcuac uuuacuaagc ccuccggcua uagcuaccac uaccaguuca guuaaugcuu 420
cucaagcagu gguacuacug aaguuucacu 450
<210> 237
<211> 2013
<212> RNA
<213> 玉米根萤叶甲属
<400> 237
uacuacguuc guuuguuagc ucagggugga uauuucaacc uuuugcuaua ucuagaaaug 60
cggcuacuau agcuccuaaa gcguguucua cugaaaccac cacuuuuaca acuagauaua 120
cugcuacauu auaggcgagg aggaccuuua uuacuguugg guccacuaag uuuaguagua 180
cgaggaggac cacgaccacu ucuaccaccu ccauuaaaac aacccagucc uccucguggg 240
uuauuauauu uaagaagacc uucuucugua gucgacauac aaccuuuaga cugaaccacc 300
uguugacuag uucuauaucu uuuacgucac guacuauauc cccauuggcu gaagguacuu 360
caauucaaaa aacuuguguc ucguuuacca guuagguucc cuaagacaca guauagaaac 420
ccuagacucc cuucguacuc ugagacggac cuugaggaua gauuuuuucu cuaguuaccg 480
guuuuagggg aacaauggga aggguguuuu guucgagaau cauugaaacu uucagucaga 540
uuuugugcgg gaagaggaug auuauuaaga uugagugcag gagggguagg auuauuauua 600
caaguaaguc caggauacgu cuuaauaccu ccaucuuacg gauacuuggg aagguacgca 660
ggguacgggg guccauacgu uccacgaggu ucuuacgucc cagguggacc uaaauuaccu 720
ggugguuugu acuuagucgu uggggggucc aagguuccau uagguguuac cuuaccugga 780
ucuggauuac caggacccgg guuauacccu uacucugggu accccggugg aguaccuguu 840
guucccgggg guucuggugg uuacgucccu gguggcgucg uuccaggagg uucuccuuac 900
ggcgguguuc cagguggcgu cuacgcaggu cuuaccuuag cuggugguua cguuguuccc 960
augggaguuc cgggcggcgu auacguuccu ggauuguacc cagguucucc aggugggguu 1020
uacccuggug ggccccgcgg aguugucguu ccuggucgag gcgugcauuu aggucguaag 1080
aaaguuguuc cuccuccugg uggcgggggu uacguugugu acggaccugg ucccgggcag 1140
uacggaggag uuccuggggg cguuccaggu ggugugccug ggcaaccugg aggugugccg 1200
ggugguaacc caggucgcuu acaaggcgga guaccuggug gagugccuau accaggugga 1260
cgucgcuacg gugucggcgg uaugccaccg gguggagguc ugguggcgcg acucuaagga 1320
gucaauuguc ucguucucaa acuccuauau uacagggccu uaucuuguca aaggucaagc 1380
cgcuaacccg cccggcauag gcugcggcgu cgaccucuua aacguucgcg guaacucuga 1440
aaccaaugac gauaaaguga guaguuuguu agguuucacc gauugcugcu agcaacguuc 1500
uaggaauauu caagcgacgu ucuaugaaac gcaccacagc uucuguuuuc gaugucgagg 1560
ucggcgucuc uggccaguuc uaggucccug ucuaguguau cuucuugauc uucucuugcu 1620
aggagcagug ccaugucucu gucuucgucu cucucccucg cacuugcgcu aucucuagca 1680
cuagcacuug cacugucuau aauacuaucu augucgcuuu cucuuucucu ggcucuagca 1740
aguucuucgu cucuuucuug ucuuucccua ucucuugcuc uaauaucucu ggcccuuggg 1800
cuucucuguc uauuucuuuu uagauuucau aggucucaga guucuaguuc uuuguuuaga 1860
ggccuuggac agcuuggauc gucgcuccau ggcuuuagua gggcgauaau acuccuaucc 1920
auagcccuug cucucucucu uccagcugcu cucucgcuag cgcuuucucu uucucuaucu 1980
ucuccccuuc ugucgguauc cagagcugug auc 2013
<210> 238
<211> 765
<212> RNA
<213> 玉米根萤叶甲属
<400> 238
uacucaagau aaccuugacc caugcuaaau agucgaaggg uuaagagagg acuaccuucu 60
cauaaaguuc aacuuauacg uuacuuucgu caacuuuuau caccguggca uuauccggag 120
gcuccauguc uaccguaaca uaaccgacga cuuuucgagu aauacaguuu uaacguacuu 180
gguucauguu uauuugcuua aaaguuguaa cuauuugugu auccuuaccg uaaaaguccg 240
aauuaucgac uacguuccgu uuagcaacuc uaacgaucuu uucuucguag uuuaauaucu 300
guaguuauac caaguuuaua aggagaauuu auggauuuac uaucucauuc guacauguac 360
guacguaugu gaaauauguc acgacaaucu gguaaaccaa cgucacagua gaaccgguca 420
auacuucuau cacuggguag auacauagac uaacuaggua gaccucaauc gauaccuaug 480
aaaccuacac gauguccauu ucguuuuguc agacguuucu gacuuuaucu uuuuaacuuc 540
uaccccuuag auuguacguu ucuugaacaa uuucuucguc gguuuuagua aauaaaccag 600
guacuacucg acuuccuauu cuuaaaacuu gaccuugaaa guacccauac guuucuaugc 660
uuaccaaaug uaugguuuca cggacuuagu cacaaacgac uacgucuuuu ucgacgguuu 720
guucguuacc uucgucuaag ucuuagucua ugucuucuau acauu 765
<210> 239
<211> 744
<212> RNA
<213> 玉米根萤叶甲属
<400> 239
uaccgaaguu uucugucuaa cuacuaaaaa gguagaucuc cucggguuua cuacuacuuu 60
agguccgauu ucccucgggu uuucccugua ucaaauaauu ucuucuuucg acuacgaaau 120
guuuacucua aaucuuacua aaacuuguuu uaauaacucu gguuuugaga guacccacuu 180
cauuacuuuc uucgacggaa aagaaaucga cuucguuuca aacguugacc acugaaguua 240
guucaacaag aaguuuuaca gugguuccga guuuauuuuu auucuugauu cuuucuguug 300
caacgaccac aaugaaaugg ucacaaacuu acgaugguuc uaccaugucu auguauacuc 360
aaccgaccaa accgaucccc uccaguuguu aacuguuuug aguucuucuu aauaguuuca 420
cgacaauuug acaaccaacu uaaucggaga aacguuugaa gaaaacauug agaacuacua 480
cauuaguuuu auuguuuguc uucucaguua cgguaacuug uacaauagua agguucuuau 540
cucgcaugaa accgaaugua guauaggcuu gaccugcuca aucuuucucu ccucaagaua 600
ucuaauuucu ucuagguccu guuuuucuuc uagucgucuc guuuccggcu cuuuguuuuu 660
guucgagaag agguucgacc cgaugaauuu cucagggucc guuuguacga aaaccuacuc 720
ccgcuacuuc uagaugaaaa gauc 744
<210> 240
<211> 4818
<212> RNA
<213> 玉米根萤叶甲属
<400> 240
uaccuccgcc gaggguucaa uggcuacgag agcaagcuca auuuaugaac augucucuug 60
caggugaaac cgggggucaa uuuugucaua uaacgacgaa aaauaccacu ucuaggucuu 120
aggauguaau gucuuuaguc guuagaacuu aggaauucua gucgacguca agcugguagu 180
ugccuacauu uaccacaugu ugacaacuuu uucauaaaga cagucgaagc aaaagaguuu 240
agauccaaag gguaccuucu cuuaguucua cgacgucaag auaaaaguac cuuuuuauua 300
cuuaaucugu auugaaguug uaggucacua uagucuauac uuaauuacca uuauuacaua 360
uuguaaccuc ggaaugugug aaaagaaccu cgguugcuga guucuuuguu aggccuacca 420
uacuuuuacc guacaugagu aaaaguuaca cgacguaccc gaaaaguuuu gcauuuucuu 480
uucauggugg uuaaguauag uuuguagagu aaccaucuug accaaguaaa aaaaguuguu 540
cagacaaauc gaguccgagu ccucacauau aaucucuucu cguacaaacu guuauccuuu 600
ggaugguagu aacguuuuca acgauagguu cagaugucaa uaaugucugu cagaaaugca 660
cagaaccuua gucauuuacu ucggaugaaa ucccuauuuu ggauguuccu caccuacuuu 720
auagaaguua aguucgacug gaugauguuu acguagagca aggauaaggu ucccguuguu 780
cgacuccuug uugucuuuua cccucuuucc caacguaaga uaguucgacg uacacuuguc 840
gaccugcucc guuucuuuua acgacgaugu aauuuuuugg uugugguggu cguucuuuau 900
uuacucccug aucguaagug augacuacac caacuuccau uuucuuuucg ucgauuuuua 960
cuuuuacuca aguagauggu acuuagucac ggacuauuuc ugguuaacgg ucuccaauuc 1020
ccacgaagua aucaguuucc uuaugguaag ucauauuuac uauaucuuca aaguccuggu 1080
cuauaaaaga gggcuaacca ggguuaccuc cgugugcuuc gucgaaggaa caugucgcuc 1140
uucuuucgag ucucuaauuc ugucuagccc cuugaauaac uuuuauuucu aguuuguaac 1200
cgacuuaaau acagcaguua cgucgaucua gauaacuggu ucuacguggu ccgaugaccu 1260
uauggcgucc ucaacuaacu aucucgucgc cgagauagac gauuuggauu gcgguaaguu 1320
cuagaauauu cacgauaccc uuucgauaga uuauauaugg uucugcaacu ucguucaaac 1380
uuacucuaac uaagaaauaa uuuccggcuu cuuguuucac uuguuuucau gguucuuugc 1440
uaaccauuuu cugguggcuc guaaaaucga ugucuaaauu gaucccuucg ccguuuuaug 1500
ucccuccgag uaugauuucg cuugcugagu uugguuugaa augugucccg cuacuaccga 1560
gugcaccgau uagacuuuua ugagguuguu ggcgauuucg ucgacguugu agucgacggg 1620
aaacagcuca aagguuuagg uuuauagcug cuuuuuagaa acuuucuaga ccuucgcgau 1680
caacguuuuc aucugcuuua cucuuggguu ucucgguacg auacccgagu ugaagcucuu 1740
agauaagugg uucugcuaua auguucaagg gaacauugcu uuguugguuu agucagcgac 1800
cuugucgaca aggucguucu ugaaguuuuc guaguuuuag acuaaagcaa cuaacuuguu 1860
uuguggagcc guguucuuuu guaauucucg cggaaucagc uaagaaugcg aaugcgacau 1920
uuaaguucuu uuauguaggu ucuauaugag guuuucucuu gguguuggua uugcagugac 1980
uaucguagca agcugagaau gcuucugaau aaccguuuuc gauuguuucc cuaucucaaa 2040
augaguuuug aacuuugcuu gcauagguuc aaugacguuu cuuauuccuc auggacguuu 2100
caaguuguuc uucucgcucu agucuacuac agcugacgcg uucacggagu uacccucuca 2160
guaugcagug aacggcgagg auuugacuuu cuaaugaacc ugagguccuu cuucucacga 2220
cgcauaagcc ucggcucaca aguugguguu gucgguugaa auucaaugag ucgacgauac 2280
cuagacggag gaccauaauc cggcugaggu caaccuaguc uuuauugccu acauggcuuu 2340
uuauaaguuc cacuuggugu uccaauauaa gguauauuaa ugguuguugg aagacaagga 2400
cggagugucu uauaacuacu ucucugauaa uuuuuuuacu ugcguaacua cgguccacga 2460
uucugcaguc acggaucagu caugccuaug ucguugaugu aaggugguug uaugggaguu 2520
ucacgcaugg uugguccauu agucagaaug ccuuuucuuu gaggucuaua auugaguggc 2580
augcugggau gguuccgcaa gugccgauga ugauugcgaa uagcaagcca cgucucgagg 2640
aguugaguuc cuaugcaugg caugcgucuu agauugcaaa gcuuacaacu gucucaaccu 2700
auaggaucgu ccauagucau gguuguucau ggacucuauc gaugaugagg ucgacuaggg 2760
uuauaauuac gcaugauggg uguacccaug aguggcucgg ucuuaaaugg cuuacgauua 2820
ugaguuguau aauggccggu ugacuucaug guaagccacc ucaugcgaag aagacacggc 2880
uuguuguagu uaauauuguc gagauggaug agcagcggcg aaagauuaua cagaucaugg 2940
uuaaggaguu uaggaucauu gaacuuauua agaaugcuca ugaugauacu gggcuuaugg 3000
ucaucaccac gucauggcuu acgaaguuuc ggaguuguca gcucgcgguc gagacguuug 3060
ggcucauggc gauacuuguu gauguuaaua augauguuau guucaugguc gccaucacau 3120
cgucgccuau gaaguuuuua uguuguuguu guugucaugg guccaugagu cuacucaguu 3180
cgcgucauga uagggcgguu acggucaaua augaguuggu caugaauguu augguugcag 3240
guuccaccau gguuagggag caugcguuga ccuguuugua uauuaguuca cuguggucac 3300
uggagaguuu uacaaagagu uuuaauguug aaauugguuc aaccaagacc ucguccugug 3360
gucguaguca ugaugagcag gcgauugcgg cgucauggua ggguuguucg acauuuauug 3420
ucaaguaaug guuugaugcc uaugcuaguc auaaugccgu uguugauauu aaggucaguu 3480
ggcucaugga ugucgcguuu gguucgugga ggacgucgug uucgacgagg uucauuauaa 3540
ggacgacggu gguuuaggag gagauuacac cuagacgagu caccgaaccu gaagucguau 3600
ucgguucgag gagaucacgg aguuguuuug uaaugcuauu uugggguucu uuuccuuugu 3660
uuugguggcc gacaaagaag acuuugguuu uugguucuag guugugguca uuggugcggg 3720
uuuguugggu gaccuggucu ucauuucgcg aacaugcagu uuuaggacgg cucguuuggg 3780
aauuuguugc uacuacacuu cuuuaacaag ccgguucucg accuguccau acucuucaag 3840
caccucugga acuggguguu uugaaacucg ccaggcuggu gagaccuaua auuuaccuuc 3900
cucuagguuc uggucguccu aacgcucggc gucuucuucu aguaaaggca gcgaucuaca 3960
auaggauacu ucuuauccaa gggccuauag aacggaaugc ugaaaagguc ccaccucaac 4020
acgcuaucau uucuacuaau auaguugcga aguauguaau uccuauagag cgguauacga 4080
ggcaguaaau aacaaugugu ucacggcaac agaaguugac aaccacuaua caccugcuac 4140
uaaucucuug uuguccagcu ugacuaggag acaaaccauu ugcuguuacu cuagccacuu 4200
cuauaaauga ccggguuucu uuuuccguca ucagaauugu augaauugua ccaguauugc 4260
aacguuuugc aauaucaauu cagaguaacc ugacuuucug acuaucgcua uuugaaugga 4320
cuuuuugccc ucagggcaca cuauuacgua gauguuaaau guagcaccgg accgucgaac 4380
aaagguuguu uaggacuugg caagcagucg auguggaacc uuagguaguu ggauaugguu 4440
guugucuucu gguugugggu aggccaccac cagguaacaa guagaccgua uccuucuucg 4500
ccggacgaga caaaugacug ucgacgauac aagcuacgac gguuguuacg auugagcuau 4560
ggucuagaau gucgaaacuc auaguucaac agguuaacgu ccuucuuaua agagucucua 4620
gcucucguaa acuucaaacc aaugcuuuca aaaaaccgca uauaauccgu auaucaaaca 4680
cuucuauuuc ggucuuucuu ugacuugcuc uaggucgggu uccaauuccu ccuuggugac 4740
cuugguggac aguaucaagg ucuugguuua uaucuaggaa auucaugaaa ucuggguaaa 4800
acccgaucau uuucuauu 4818
<210> 241
<211> 166
<212> PRT
<213> 玉米根萤叶甲属
<400> 241
Met Thr Pro Glu Arg Lys Lys Lys Leu Arg Leu Leu Leu Arg Lys Lys
1 5 10 15
Ala Ala Glu Glu Leu Lys Lys Glu Gln Glu Arg Lys Ala Ala Glu Arg
20 25 30
Arg Arg Ile Ile Glu Glu Arg Cys Gly Lys Pro Lys Leu Val Asp Asp
35 40 45
Ala Asn Glu Gly Pro Leu Lys Gln Val Cys Glu Gly Tyr His Arg Arg
50 55 60
Ile Val Asp Leu Glu Asn Lys Lys Phe Asp Leu Glu Lys Glu Val Glu
65 70 75 80
Phe Arg Asp Phe Gln Ile Ser Glu Leu Asn Ser Gln Val Asn Asp Leu
85 90 95
Arg Gly Lys Phe Val Lys Pro Thr Leu Lys Lys Val Ser Lys Tyr Glu
100 105 110
Asn Lys Phe Ala Lys Leu Gln Lys Lys Ala Ala Glu Phe Asn Phe Arg
115 120 125
Asn Gln Leu Lys Val Val Lys Lys Lys Glu Phe Thr Leu Glu Glu Glu
130 135 140
Asp Lys Glu Lys Lys Pro Asp Trp Ser Lys Lys Gly Asp Glu Lys Lys
145 150 155 160
Val Gln Glu Ala Glu Ala
165
<210> 242
<211> 201
<212> PRT
<213> 玉米根萤叶甲属
<400> 242
Met Cys Glu Glu Glu Val Ala Ala Leu Val Val Asp Asn Gly Ser Gly
1 5 10 15
Met Cys Lys Ala Gly Phe Ala Gly Asp Asp Ala Pro Arg Ala Val Phe
20 25 30
Pro Ser Ile Val Gly Arg Pro Arg His Gln Gly Val Met Val Gly Met
35 40 45
Gly Gln Lys Asp Ser Tyr Val Gly Asp Glu Ala Gln Ser Lys Arg Gly
50 55 60
Ile Leu Thr Leu Lys Tyr Pro Ile Glu His Gly Ile Val Thr Asn Trp
65 70 75 80
Asp Asp Met Glu Lys Ile Trp His His Thr Phe Tyr Asn Glu Leu Arg
85 90 95
Val Ala Pro Glu Glu His Pro Val Leu Leu Thr Glu Ala Pro Leu Asn
100 105 110
Pro Lys Ala Asn Arg Glu Lys Met Thr Gln Ile Met Phe Glu Thr Phe
115 120 125
Asn Thr Pro Ala Met Tyr Val Ala Ile Gln Ala Val Leu Ser Leu Tyr
130 135 140
Ala Ser Gly Arg Thr Thr Gly Ile Val Leu Asp Ser Gly Asp Gly Val
145 150 155 160
Ser His Thr Val Pro Ile Tyr Glu Gly Tyr Ala Leu Pro His Ala Ile
165 170 175
Leu Arg Leu Asp Leu Ala Gly Arg Asp Leu Thr Asp Tyr Leu Met Lys
180 185 190
Ile Leu Thr Glu Arg Gly Tyr Ser Phe
195 200
<210> 243
<211> 913
<212> PRT
<213> 玉米根萤叶甲属
<400> 243
Met Pro Leu Arg Leu Asp Ile Lys Arg Lys Leu Thr Ala Arg Ser Asp
1 5 10 15
Arg Val Lys Cys Val Asp Leu His Pro Thr Glu Pro Trp Met Leu Cys
20 25 30
Ser Leu Tyr Ser Gly Asn Ile Asn Val Trp Asn Thr Glu Asn Gln Gln
35 40 45
Leu Val Lys Thr Phe Glu Val Cys Asp Val Pro Val Arg Thr Ala Lys
50 55 60
Phe Leu Pro Arg Lys Asn Trp Ile Val Ser Gly Ser Asp Asp Met Gln
65 70 75 80
Ile Arg Val Phe Asn Tyr Asn Thr Leu Asp Arg Val His Ser Phe Glu
85 90 95
Ala His Ser Asp Tyr Val Arg Cys Ile Val Val His Pro Thr Gln Pro
100 105 110
Tyr Ile Leu Thr Ser Ser Asp Asp Met Leu Ile Lys Leu Trp Asn Trp
115 120 125
Glu Lys Ala Trp Ala Cys Gln Gln Val Phe Glu Gly His Thr His Tyr
130 135 140
Ile Met Gln Ile Ala Ile Asn Pro Lys Asp Asn Asn Thr Phe Ala Ser
145 150 155 160
Ala Ser Leu Asp Arg Thr Leu Lys Val Trp Gln Leu Gly Ala Ser Thr
165 170 175
Ala Asn Phe Thr Leu Glu Gly His Glu Lys Gly Val Asn Cys Val Asp
180 185 190
Tyr Tyr His Gly Gly Asp Lys Pro Tyr Leu Ile Ser Gly Ala Asp Asp
195 200 205
Arg Leu Val Lys Ile Trp Asp Tyr Gln Asn Lys Thr Cys Val Gln Thr
210 215 220
Leu Glu Gly His Ala Gln Asn Val Thr Ala Ala Cys Phe His Pro Glu
225 230 235 240
Leu Pro Val Ala Leu Thr Gly Ser Glu Asp Gly Thr Val Arg Val Trp
245 250 255
His Ala Asn Thr His Arg Leu Glu Ser Ser Leu Asn Tyr Gly Phe Glu
260 265 270
Arg Val Trp Thr Ile Phe Cys Leu Lys Gly Ser Asn Asn Val Ala Leu
275 280 285
Gly Tyr Asp Glu Gly Ser Ile Leu Val Lys Val Gly Arg Glu Glu Pro
290 295 300
Ala Val Ser Met Asp Ala Ser Gly Gly Lys Ile Ile Trp Ala Arg His
305 310 315 320
Ser Glu Leu Gln Gln Ala Asn Leu Lys Ala Leu Ala Glu Gly Ala Glu
325 330 335
Ile Arg Asp Gly Glu Arg Leu Pro Val Ser Val Lys Asp Met Gly Ala
340 345 350
Cys Glu Ile Tyr Pro Gln Thr Ile Gln His Asn Pro Asn Gly Arg Phe
355 360 365
Val Val Val Cys Gly Asp Gly Glu Tyr Ile Ile Tyr Thr Ala Met Ala
370 375 380
Leu Arg Asn Lys Ala Phe Gly Ser Ala Gln Glu Phe Val Trp Ala Gln
385 390 395 400
Asp Ser Ser Glu Tyr Ala Ile Arg Glu Ser Gly Ser Thr Ile Arg Ile
405 410 415
Phe Lys Asn Phe Lys Glu Lys Lys Asn Phe Lys Ser Asp Phe Gly Ala
420 425 430
Glu Gly Ile Tyr Gly Gly Tyr Leu Leu Gly Val Lys Ser Val Ser Gly
435 440 445
Leu Thr Phe Tyr Asp Trp Glu Thr Leu Asp Leu Val Arg Arg Ile Glu
450 455 460
Ile Gln Pro Lys Ala Val Tyr Trp Ser Asp Ser Gly Lys Leu Val Cys
465 470 475 480
Leu Ala Thr Glu Asp Ser Tyr Phe Ile Leu Ser Tyr Asp Ser Asp Glu
485 490 495
Val Gln Lys Ala Arg Asp Asn Asn Gln Val Ala Asp Asp Gly Val Glu
500 505 510
Ser Ala Phe Asn Leu Leu Gly Glu Ile Asn Glu Ser Val Arg Thr Gly
515 520 525
Leu Trp Val Gly Asp Cys Phe Ile Tyr Thr Asn Ser Val Asn Arg Ile
530 535 540
Asn Tyr Phe Val Gly Gly Glu Leu Val Thr Ile Ala His Leu Asp Arg
545 550 555 560
Pro Leu Tyr Val Leu Gly Tyr Val Pro Lys Asp Asp Arg Leu Tyr Leu
565 570 575
Val Asp Lys Glu Leu Arg Val Val Ser Tyr Gln Leu Leu Leu Ser Val
580 585 590
Leu Glu Tyr Gln Thr Ala Val Met Arg Arg Asp Phe Pro Thr Ala Asp
595 600 605
Arg Val Leu Pro Ser Ile Pro Lys Glu His Arg Thr Arg Val Ala His
610 615 620
Phe Leu Glu Lys Gln Gly Phe Lys Gln Gln Ala Leu Ala Val Ser Thr
625 630 635 640
Asp Pro Glu His Arg Phe Glu Leu Ala Val Ala Leu Glu Asp Leu Asn
645 650 655
Ile Ala Lys Thr Leu Ala Gln Glu Ala Asn Ser Pro Gln Lys Trp Asn
660 665 670
Gln Leu Ala Glu Leu Ala Ala Ala Thr Asn Asn Val Ser Val Ala Lys
675 680 685
Glu Cys Met Gln Lys Ala Gln Asp Tyr Gly Gly Leu Leu Leu Leu Ala
690 695 700
Thr Ser Ser Gly Asp Glu Asn Leu Val Arg Thr Leu Gly Glu Thr Thr
705 710 715 720
Gln Ala Glu Ser Lys His Asn Leu Ala Phe Leu Ser His Leu Leu Val
725 730 735
Gly Asp Leu Asn Lys Cys Leu Asp Ile Leu Ile Asn Thr Gly Arg Leu
740 745 750
Pro Glu Ala Ala Phe Phe Ala Arg Ser Tyr Leu Pro Asp Lys Ile Thr
755 760 765
Glu Val Val Glu Leu Trp Lys Thr Gln Leu Ser Ser Val Asn Gln Lys
770 775 780
Ala Gly Gln Ser Leu Ala Asp Pro Lys Asn Tyr Glu Asn Leu Phe Pro
785 790 795 800
Gly Leu Gln Glu Ala Val Val Ala Gln Lys Phe Leu Glu Gln Gln Asn
805 810 815
Lys Gly Leu Ala Pro Ala Arg Val Ala Thr Thr Ile Pro Pro Asn His
820 825 830
Asp Arg Asn Val Val Ala Glu Val Gln Ala Gln Ser Lys His Asp Val
835 840 845
Pro Ser Phe Ser Ser Ser Phe Ile Ser Ser Glu Ile Glu Ala Gln Thr
850 855 860
Arg Ser Ser Ala Lys Pro Glu Glu Ser Ser Asn Ile Ile Gln Leu Asp
865 870 875 880
Gln Asp Asp Asp Asp Ile Asp Leu Asp Leu Asp Gly Val Asn Ile Asp
885 890 895
Glu Asn Ile Asp Thr Thr Asp Ile Asn Ile Asp Asp Asp Leu Leu Ser
900 905 910
Asp
<210> 244
<211> 381
<212> PRT
<213> 玉米根萤叶甲属
<400> 244
Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu
1 5 10 15
Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp
20 25 30
Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys
35 40 45
Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu
50 55 60
Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Met His Ile Phe
65 70 75 80
Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser
85 90 95
Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile
100 105 110
Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu Glu Asp
115 120 125
Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr Leu His
130 135 140
Leu Val Leu Arg Leu Arg Gly Gly Met Gln Ile Phe Val Lys Thr Leu
145 150 155 160
Thr Gly Lys Thr Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu
165 170 175
Asn Val Lys Ala Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln
180 185 190
Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu
195 200 205
Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg
210 215 220
Leu Arg Gly Gly Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr
225 230 235 240
Ile Thr Leu Glu Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala
245 250 255
Lys Ile Gln Asp Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile
260 265 270
Phe Ala Gly Lys Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn
275 280 285
Ile Gln Lys Glu Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly
290 295 300
Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu
305 310 315 320
Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp
325 330 335
Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys
340 345 350
Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu
355 360 365
Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Asn
370 375 380
<210> 245
<211> 376
<212> PRT
<213> 玉米根萤叶甲属
<400> 245
Met Cys Asp Asp Asp Val Ala Ala Leu Val Val Asp Asn Gly Ser Gly
1 5 10 15
Met Cys Lys Ala Gly Phe Ala Gly Asp Asp Ala Pro Arg Ala Val Phe
20 25 30
Pro Ser Ile Val Gly Arg Pro Arg His Gln Gly Val Met Val Gly Met
35 40 45
Gly Gln Lys Asp Ser Tyr Val Gly Asp Glu Ala Gln Ser Lys Arg Gly
50 55 60
Ile Leu Thr Leu Lys Tyr Pro Ile Glu His Gly Ile Ile Thr Asn Trp
65 70 75 80
Asp Asp Met Glu Lys Ile Trp His His Thr Phe Tyr Asn Glu Leu Arg
85 90 95
Val Ala Pro Glu Glu His Pro Ile Leu Leu Thr Glu Ala Pro Leu Asn
100 105 110
Pro Lys Ala Asn Arg Glu Lys Met Thr Gln Ile Met Phe Glu Thr Phe
115 120 125
Asn Thr Pro Ala Met Tyr Val Ala Ile Gln Ala Val Leu Ser Leu Tyr
130 135 140
Ala Ser Gly Arg Thr Thr Gly Ile Val Leu Asp Ser Gly Asp Gly Val
145 150 155 160
Ser His Thr Val Pro Ile Tyr Glu Gly Tyr Ala Leu Pro His Ala Ile
165 170 175
Leu Arg Leu Asp Leu Ala Gly Arg Asp Leu Thr Asp Tyr Leu Met Lys
180 185 190
Ile Leu Thr Glu Arg Gly Tyr Ser Phe Thr Thr Thr Ala Glu Arg Glu
195 200 205
Ile Val Arg Asp Ile Lys Glu Lys Leu Cys Tyr Val Ala Leu Asp Phe
210 215 220
Glu Gln Glu Met Ala Thr Ala Ala Ser Ser Thr Ser Leu Glu Lys Ser
225 230 235 240
Tyr Glu Leu Pro Asp Gly Gln Val Ile Thr Ile Gly Asn Glu Arg Phe
245 250 255
Arg Cys Pro Glu Ala Leu Phe Gln Pro Ser Phe Leu Gly Met Glu Ser
260 265 270
Cys Gly Ile His Glu Thr Val Tyr Asn Ser Ile Met Lys Cys Asp Val
275 280 285
Asp Ile Arg Lys Asp Leu Tyr Ala Asn Thr Val Leu Ser Gly Gly Thr
290 295 300
Thr Met Tyr Pro Gly Ile Ala Asp Arg Met Gln Lys Glu Ile Thr Ala
305 310 315 320
Leu Ala Pro Ser Thr Ile Lys Ile Lys Ile Ile Ala Pro Pro Glu Arg
325 330 335
Lys Tyr Ser Val Trp Ile Gly Gly Ser Ile Leu Ala Ser Leu Ser Thr
340 345 350
Phe Gln Gln Met Trp Ile Ser Lys Gln Glu Tyr Asp Glu Ser Gly Pro
355 360 365
Gly Ile Val His Arg Lys Cys Phe
370 375
<210> 246
<211> 879
<212> PRT
<213> 玉米根萤叶甲属
<400> 246
Met Gly Thr Phe Lys Arg Asp Thr His Asp Glu Asp Gly Gly Ser Ser
1 5 10 15
Ala Phe Gln Asn Leu Glu Lys Thr Thr Val Leu Gln Glu Ala Arg Val
20 25 30
Phe Asn Glu Thr Ser Val Asn Pro Arg Lys Cys Thr Pro Ile Leu Thr
35 40 45
Lys Leu Leu Tyr Leu Leu Asn Gln Gly Glu Thr Leu Ser Ala Lys Glu
50 55 60
Ala Thr Asp Val Phe Phe Ala Met Thr Lys Leu Phe Gln Ser Lys Asp
65 70 75 80
Val Ile Leu Arg Arg Met Val Tyr Leu Gly Ile Lys Glu Leu Ser Ser
85 90 95
Val Ala Asp Asp Val Ile Ile Val Thr Ser Ser Leu Thr Lys Asp Met
100 105 110
Thr Gly Lys Glu Asp Met Tyr Arg Ala Ala Ala Ile Arg Ala Leu Cys
115 120 125
Ser Ile Thr Asp Ala Thr Met Leu Gln Ala Ile Glu Arg Tyr Met Lys
130 135 140
Gln Ala Ile Val Asp Arg Asn Ala Ala Val Ser Ser Ala Ala Leu Ile
145 150 155 160
Ser Ser Leu His Met Ser Lys Leu Ala Pro Asp Val Val Lys Arg Trp
165 170 175
Val Asn Glu Ala Gln Glu Ala Val Asn Ser Asp Asn Ala Met Val Gln
180 185 190
Tyr His Ala Leu Gly Leu Leu Tyr His Ile Arg Lys Thr Asp Lys Leu
195 200 205
Ala Val Thr Lys Leu Ile Ser Lys Leu Asn Ser Met Gly Leu Lys Ser
210 215 220
Pro Tyr Ala Leu Cys Met Leu Ile Arg Ile Thr Ala Lys Leu Leu Glu
225 230 235 240
Glu Glu Asp Gln Glu Ser Leu Leu Asn Ser Pro Tyr Thr Ile Ile Phe
245 250 255
Thr Met Gly Leu Arg Asn Lys Ser Glu Met Val Val Tyr Glu Ala Ala
260 265 270
His Ala Met Val Asn Leu Lys Phe Thr Ser Ser Asn Val Leu Ala Pro
275 280 285
Ala Ile Ser Val Leu Gln Leu Phe Cys Gly Ser Pro Lys Ala Thr Leu
290 295 300
Arg Phe Ala Ala Val Arg Thr Leu Asn Gln Val Ala Thr Thr His Pro
305 310 315 320
Ala Ser Val Thr Ala Cys Asn Leu Asp Leu Glu Asn Leu Ile Thr Asp
325 330 335
Pro Asn Arg Ser Ile Ala Thr Leu Ala Ile Thr Thr Leu Leu Lys Thr
340 345 350
Gly Ala Glu Ser Ser Val Asp Arg Leu Met Lys Gln Ile Ala Thr Phe
355 360 365
Val Ser Glu Ile Ser Asp Glu Phe Lys Val Val Val Ile Gln Ala Ile
370 375 380
Lys Val Leu Ala Leu Lys Phe Pro Arg Lys His Ser Thr Leu Met Asn
385 390 395 400
Phe Leu Ser Ala Met Leu Arg Asp Glu Gly Gly Leu Glu Tyr Lys Ala
405 410 415
Ser Ile Ala Asp Thr Ile Ile Thr Leu Ile Glu Asp Asn Pro Glu Ala
420 425 430
Lys Glu Ser Gly Leu Ala His Leu Cys Glu Phe Ile Glu Asp Cys Glu
435 440 445
His Val Ser Leu Ala Val Arg Ile Leu His Leu Leu Gly Lys Glu Gly
450 455 460
Pro Lys Thr Lys Gln Pro Ser Arg Tyr Ile Arg Phe Ile Tyr Asn Arg
465 470 475 480
Val Ile Leu Glu Cys Pro Ser Val Arg Ala Ala Ala Val Ser Ala Met
485 490 495
Ala Gln Phe Gly Ala Ser Cys Pro Asp Leu Leu Glu Asn Ile Gln Ile
500 505 510
Leu Leu Ser Arg Cys Gln Met Asp Ser Asp Asp Glu Val Arg Asp Arg
515 520 525
Ala Thr Tyr Tyr Ser Asn Ile Leu Asn Lys Asn Asp Lys Ser Leu Tyr
530 535 540
Asn Asn Tyr Ile Leu Asp Ser Leu Gln Val Ser Ile Pro Ser Leu Glu
545 550 555 560
Arg Ser Leu Arg Glu Tyr Ile Gln Asn Pro Thr Asp Glu Pro Phe Asp
565 570 575
Ile Lys Ser Val Pro Val Ala Ala Val Pro Thr Ala Glu Glu Arg Glu
580 585 590
Val Lys Asn Lys Ser Glu Gly Leu Leu Val Ser Gln Gly Pro Val Arg
595 600 605
Pro Pro Pro Val Ser Arg Glu Glu Asn Phe Ala Glu Lys Leu Ser Asn
610 615 620
Val Pro Gly Ile Gln Gln Leu Gly Pro Leu Phe Lys Thr Ser Asp Val
625 630 635 640
Val Glu Leu Thr Glu Ser Glu Thr Glu Tyr Phe Val Arg Cys Ile Lys
645 650 655
His Cys Phe Lys His His Ile Val Leu Gln Phe Asp Cys Leu Asn Thr
660 665 670
Leu Pro Asp Gln Leu Leu Glu Asn Val Arg Val Glu Ile Asp Ala Gly
675 680 685
Glu Thr Phe Glu Ile Leu Ala Glu Ile Pro Cys Glu Lys Leu His Tyr
690 695 700
Asn Glu Thr Gly Thr Thr Tyr Val Val Val Lys Leu Pro Asp Asp Asp
705 710 715 720
Leu Pro Asn Ser Val Gly Thr Cys Gly Ala Val Leu Lys Phe Leu Val
725 730 735
Lys Asp Cys Asp Pro Ser Thr Gly Ile Pro Asp Ser Asp Glu Gly Tyr
740 745 750
Asp Asp Glu Tyr Thr Leu Glu Asp Ile Glu Ile Thr Leu Gly Asp Gln
755 760 765
Ile Gln Lys Val Ser Lys Val Asn Trp Ala Ala Ala Trp Glu Glu Ala
770 775 780
Ala Ala Thr Tyr Val Glu Lys Glu Asp Thr Tyr Ser Leu Thr Ile Asn
785 790 795 800
Thr Leu Ser Gly Ala Val Lys Asn Ile Ile Gln Phe Leu Gly Leu Gln
805 810 815
Pro Ala Glu Arg Thr Asp Arg Val Pro Glu Gly Lys Ser Thr His Thr
820 825 830
Leu Leu Leu Ala Gly Val Phe Arg Gly Gly Ile Asp Ile Leu Val Arg
835 840 845
Ala Lys Leu Ala Leu Gly Glu Cys Val Thr Met Gln Leu Thr Val Arg
850 855 860
Ser Pro Asp Pro Asp Val Ala Glu Leu Ile Thr Ser Thr Val Gly
865 870 875
<210> 247
<211> 216
<212> PRT
<213> 玉米根萤叶甲属
<400> 247
Met Ala Ala Asn Arg Thr Gly Pro Ala Gln Arg Pro Asn Gly Ala Thr
1 5 10 15
Gln Gly Lys Ile Cys Gln Phe Lys Leu Val Leu Leu Gly Glu Ser Ala
20 25 30
Val Gly Lys Ser Ser Leu Val Leu Arg Phe Val Lys Gly Gln Phe His
35 40 45
Glu Tyr Gln Glu Ser Thr Ile Gly Ala Ala Phe Leu Thr Gln Thr Ile
50 55 60
Cys Leu Asp Asp Thr Thr Val Lys Phe Glu Ile Trp Asp Thr Ala Gly
65 70 75 80
Gln Glu Arg Tyr His Ser Leu Ala Pro Met Tyr Tyr Arg Gly Ala Gln
85 90 95
Ala Ala Ile Val Val Tyr Asp Ile Thr Asn Gln Asp Thr Phe Gly Arg
100 105 110
Ala Lys Thr Trp Val Lys Glu Leu Gln Arg Gln Ala Ser Pro Thr Ile
115 120 125
Val Ile Ala Leu Ala Gly Asn Lys Gln Asp Leu Ala Asn Lys Arg Met
130 135 140
Val Glu Tyr Glu Glu Ala Gln Thr Tyr Ala Asp Glu Asn Gly Leu Leu
145 150 155 160
Phe Met Glu Thr Ser Ala Lys Thr Ala Met Asn Val Asn Asp Ile Phe
165 170 175
Leu Ala Ile Ala Lys Lys Leu Pro Lys Asn Glu Gln Thr Thr Gly Gln
180 185 190
Gly Gly Ser Ala Gln Gly Arg Arg Leu Ala Glu Gly Asp Ser Gly Ala
195 200 205
Lys Ala Pro Gly Asn Cys Cys Lys
210 215
<210> 248
<211> 92
<212> PRT
<213> 玉米根萤叶甲属
<400> 248
Met Lys Phe Leu Arg Ser Thr Val Cys Tyr Ile Ala Ile Leu Ala Ile
1 5 10 15
Leu Phe Thr Leu Cys Ala Asp Glu Val Glu Gly Arg Arg Lys Ile Leu
20 25 30
Met Gly Arg Lys Ser Ile Thr Arg Thr Tyr Leu Arg Gly Asn Ala Val
35 40 45
Pro Ala Tyr Val Ile Ile Ile Leu Val Gly Ile Gly Gln Ile Ile Leu
50 55 60
Gly Gly Ile Leu Tyr Val Ala Leu Arg Lys Lys Ile Ile Ala Ala Pro
65 70 75 80
Val Thr Ala Ser Tyr Ala Val Ala Arg Gln Glu Pro
85 90
<210> 249
<211> 157
<212> PRT
<213> 玉米根萤叶甲属
<400> 249
Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu
1 5 10 15
Val Glu Pro Ser Asp Thr Ile Glu Asn Val Lys Ala Lys Ile Gln Asp
20 25 30
Lys Glu Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys
35 40 45
Gln Leu Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu
50 55 60
Ser Thr Leu His Leu Val Leu Arg Leu Arg Gly Gly Ala Lys Lys Arg
65 70 75 80
Lys Lys Lys Asn Tyr Ser Thr Pro Lys Lys Ile Lys His Lys Lys Lys
85 90 95
Lys Val Lys Leu Ala Val Leu Lys Phe Tyr Lys Val Asp Glu Asn Gly
100 105 110
Lys Ile His Arg Leu Arg Arg Glu Cys Pro Ala Glu Gln Cys Gly Ala
115 120 125
Gly Val Phe Met Ala Ala Met Glu Asp Arg His Tyr Cys Gly Lys Cys
130 135 140
Gly Tyr Thr Leu Val Phe Ser Lys Pro Gly Asp Glu Lys
145 150 155
<210> 250
<211> 248
<212> PRT
<213> 玉米根萤叶甲属
<400> 250
Met Met Ser Lys Ala Asp Thr Gln Glu Asp Ala Ser Phe Ala Lys Leu
1 5 10 15
Glu Asn Gln Ile Ala Ile Ile Lys Tyr Val Ile Leu Phe Thr Asn Val
20 25 30
Leu Gln Trp Ala Leu Gly Ala Ala Ile Phe Ala Leu Cys Leu Trp Leu
35 40 45
Arg Phe Glu Glu Gly Ile Gln Glu Trp Leu Gln Lys Leu Asp Ser Glu
50 55 60
Gln Phe Tyr Ile Gly Val Tyr Val Leu Ile Val Ala Ser Leu Ile Val
65 70 75 80
Met Ile Val Ser Phe Ile Gly Cys Ile Ser Ala Leu Gln Glu Ser Thr
85 90 95
Met Ala Leu Leu Val Tyr Ile Gly Thr Gln Val Leu Ser Phe Ile Phe
100 105 110
Gly Leu Ser Gly Ser Ala Val Leu Leu Asp Asn Ser Ala Arg Asp Ser
115 120 125
His Phe Gln Pro Arg Ile Arg Glu Ser Met Arg Arg Leu Ile Met Asn
130 135 140
Ala His His Asp Gln Ser Arg Gln Thr Leu Ala Met Ile Gln Glu Asn
145 150 155 160
Val Gly Cys Cys Gly Ala Asp Gly Ala Thr Asp Tyr Leu Ser Leu Gln
165 170 175
Gln Pro Leu Pro Ser Gln Cys Arg Asp Thr Val Thr Gly Asn Pro Phe
180 185 190
Phe His Gly Cys Val Asp Glu Leu Thr Trp Phe Phe Glu Glu Lys Cys
195 200 205
Gly Trp Ile Ala Gly Leu Ala Met Ala Ile Cys Met Ile Asn Val Leu
210 215 220
Ser Ile Val Leu Ser Thr Val Leu Ile Gln Ala Leu Lys Lys Glu Glu
225 230 235 240
Glu Ala Ser Asp Ser Tyr Arg Arg
245
<210> 251
<211> 124
<212> PRT
<213> 玉米根萤叶甲属
<400> 251
Met Ser Gly Arg Gly Lys Gly Gly Lys Val Lys Gly Lys Ala Lys Ser
1 5 10 15
Arg Ser Asn Arg Ala Gly Leu Gln Phe Pro Val Gly Arg Ile His Arg
20 25 30
Leu Leu Arg Lys Gly Asn Tyr Ala Glu Arg Val Gly Ala Gly Ala Pro
35 40 45
Val Tyr Leu Ala Ala Val Met Glu Tyr Leu Ala Ala Glu Val Leu Glu
50 55 60
Leu Ala Gly Asn Ala Ala Arg Asp Asn Lys Lys Thr Arg Ile Ile Pro
65 70 75 80
Arg His Leu Gln Leu Ala Ile Arg Asn Asp Glu Glu Leu Asn Lys Leu
85 90 95
Leu Ser Gly Val Thr Ile Ala Gln Gly Gly Val Leu Pro Asn Ile Gln
100 105 110
Ala Val Leu Leu Pro Lys Lys Thr Glu Lys Lys Ala
115 120
<210> 252
<211> 288
<212> PRT
<213> 玉米根萤叶甲属
<400> 252
Met Lys Leu Asp Gly Val Asp Leu Pro Pro Pro Ile Ser Phe Asp Ile
1 5 10 15
Ala Glu Glu Gln Pro Leu Pro Pro Cys Gln Gln Thr Phe Leu Cys Asn
20 25 30
Gly Asp Gly Gly Ser Ile Val Arg Gln Phe Leu Glu Leu Tyr Phe Val
35 40 45
Ile Tyr Asp Ser Asp Asn Arg Gln Ser Leu Leu Gln Ala Tyr His Glu
50 55 60
Lys Ala Thr Phe Ser Met Thr Met Ala Tyr Pro Tyr Gly Tyr Ser Lys
65 70 75 80
Asp Ser Lys Gly Val Ser Trp Leu Asn Trp Tyr Ala Thr Asp Asn Arg
85 90 95
Asn Leu Leu Arg Val Gln Asp Pro Asp Arg Arg Asn Lys Leu Leu Arg
100 105 110
Gln Gly Gln Val Ala Val Val Ser Phe Leu Gln Asp Met Pro His Thr
115 120 125
Lys His Asp Ile His Ser Phe Thr Val Asp Leu Thr Val Phe Thr Pro
130 135 140
Gln Met Leu Cys Leu Thr Val Ala Gly Met Phe Lys Glu Leu Lys Ser
145 150 155 160
Gly His Lys Val Pro Pro Leu Arg Tyr Phe Phe Arg Thr Leu Val Ile
165 170 175
Val Pro Ala Gly Ser Gly Phe Cys Ile Ala Asn Glu Glu Leu His Ile
180 185 190
Ser Asn Ala Thr Pro Asp Gln Ala Lys Asp Ala Phe Lys Thr Thr Val
195 200 205
Asn Val Ala Pro Ala Pro Ala Pro Val Ile Thr Ser Pro Gly Pro Ser
210 215 220
Ile Pro Gln Pro Ala Val Pro Asp Asp Ala Thr Lys Gln Glu Met Val
225 230 235 240
Lys Gln Met Ser Ala Val Ser Gly Met Asn Leu Glu Trp Ser Leu Gln
245 250 255
Cys Leu Glu Glu Thr Gln Trp Asp Tyr Gln Lys Ala Ile Met Val Phe
260 265 270
Gln Asn Leu Asn Ala Gln Gly Val Val Pro Gln Ala Ala Phe Ile Lys
275 280 285
<210> 253
<211> 955
<212> PRT
<213> 玉米根萤叶甲属
<400> 253
Met Thr Ala Val Glu Gln Pro Cys Tyr Thr Leu Ile Asn Leu Pro Thr
1 5 10 15
Asp Ser Glu Pro Tyr Asn Glu Met Gln Leu Lys Met Asp Leu Glu Lys
20 25 30
Gly Glu Val Lys Val Lys Ile Arg Ala Leu Glu Lys Ile Ile His Met
35 40 45
Ile Leu Ala Gly Glu Arg Leu Pro Asn Gly Phe Leu Met Thr Ile Ile
50 55 60
Arg Asn Val Leu Pro Leu Gln Asp His Leu Ala Lys Lys Leu Leu Leu
65 70 75 80
Ile Phe Trp Glu Ile Val Pro Lys Thr Asn Pro Glu Gly Lys Leu Leu
85 90 95
Gln Glu Met Ile Leu Val Cys Asp Ala Tyr Arg Lys Asp Leu Gln His
100 105 110
Pro Asn Glu Phe Leu Arg Gly Ser Thr Leu Arg Phe Leu Cys Lys Leu
115 120 125
Lys Glu Pro Glu Leu Leu Glu Pro Leu Met Pro Ser Ile Arg Ala Cys
130 135 140
Leu Asp His Arg His Ser Tyr Val Arg Arg Asn Ala Val Leu Ala Ile
145 150 155 160
Phe Thr Ile Tyr Lys Asn Phe Glu Ala Leu Ile Pro Asp Ala Pro Glu
165 170 175
Leu Ile Ser Asn Tyr Leu Asp Gly Glu Gln Asp Met Ser Cys Lys Arg
180 185 190
Asn Ala Phe Leu Met Leu Leu His Ala Asp Gln Glu Arg Ala Leu Ser
195 200 205
Tyr Leu Ala Ser Cys Leu Asp Gln Val Asn Ser Phe Gly Asp Ile Leu
210 215 220
Gln Leu Val Ile Val Glu Leu Ile Tyr Lys Val Cys His Ser Asn Pro
225 230 235 240
Ala Glu Arg Ser Arg Phe Ile Arg Cys Ile Tyr Asn Leu Leu Asn Ser
245 250 255
Ser Ser Pro Ala Val Arg Tyr Glu Ala Ala Gly Thr Leu Val Thr Leu
260 265 270
Ser Ser Ala Pro Thr Ala Val Lys Ala Ala Ala Ser Cys Tyr Ile Glu
275 280 285
Leu Ile Ile Lys Glu Ser Asp Asn Asn Val Lys Leu Ile Val Leu Asp
290 295 300
Arg Leu Ile Ala Leu Lys Glu Leu Pro Asn His Glu Arg Ile Leu Gln
305 310 315 320
Asp Leu Val Met Asp Ile Leu Arg Val Leu Ser Ala Pro Asp Leu Glu
325 330 335
Val Arg Lys Lys Thr Leu Ser Leu Ala Leu Glu Leu Val Ser Ser Arg
340 345 350
Asn Ile Glu Glu Met Val Leu Val Leu Thr Lys Glu Val Ser Lys Thr
355 360 365
Val Asp Ser Glu His Glu Asp Thr Gly Lys Tyr Arg Gln Leu Leu Val
370 375 380
Arg Thr Leu His Ser Cys Ser Ile Lys Phe Pro Asp Ile Ala Arg Ser
385 390 395 400
Val Ile Pro Val Leu Ile Glu Phe Leu Ser Asp Asn Asn Glu Leu Ala
405 410 415
Ala Thr Asp Val Leu Leu Phe Leu Arg Glu Ala Ile Gln Lys Phe Lys
420 425 430
Glu Leu Gln Pro Leu Ile Ile Glu Lys Leu Ile Glu Thr Phe Lys Asp
435 440 445
Ile Lys Leu Val Lys Val His Arg Ala Ala Ile Trp Ile Leu Gly Glu
450 455 460
Tyr Ala Ser Thr Ala Ser Asp Ile Glu Val Ile Val Gly Glu Ile Asn
465 470 475 480
Arg Leu Leu Gly Glu Gly Ser Leu Val Glu Ala Glu Gln Lys Leu Ile
485 490 495
Ala Gly Asp Thr Glu Glu Asn Ala Pro Ala Pro Ala Ala Gly Ala Thr
500 505 510
Thr Leu Val Thr Ser Asp Gly Thr Tyr Ala Thr Gln Ser Ala Phe Asn
515 520 525
Thr Val Ser Gln Thr Thr Lys Glu Ala Arg Pro Pro Leu Arg Gln Tyr
530 535 540
Leu Met Asp Gly Asp Phe Phe Ile Gly Ala Ser Leu Ala Ser Thr Leu
545 550 555 560
Thr Lys Leu Ser Leu Arg Tyr Glu Asp Leu Thr Ser Pro Ala Ala Ser
565 570 575
Asn Gly Phe Asn Ala Lys Ile Met Leu Ile Met Ala Gly Ile Leu His
580 585 590
Leu Gly Lys Ser Gly Leu Pro Thr Lys Ser Ile Thr Asn Asp Asp Lys
595 600 605
Asp His Ile Leu Phe Cys Leu Arg Val Leu Ser Asp Arg Ser Pro Ile
610 615 620
Ile Val Glu Ile Phe Lys Lys Leu Cys Arg Ser Ala Leu Asn Glu Met
625 630 635 640
Leu Leu Ala Lys Glu Ser Val Glu Ala Ile Ser Gln Lys Ser Lys Glu
645 650 655
Lys Asn Lys Arg Thr Ile Gln Thr Asp Asp Ala Ile Ser Phe Leu Gln
660 665 670
Leu Glu Thr Asp Lys Ser Gly Glu Leu Gly Glu Asn Val Phe Glu Met
675 680 685
Ser Leu Ser Gln Ala Leu Val Gly Gly Arg Thr Gly Gly Gly Glu Ser
690 695 700
Val Leu Ser Ser Asn Lys Leu Asp Lys Ile Thr Gln Leu Thr Gly Phe
705 710 715 720
Ser Asp Pro Val Tyr Ser Glu Ala Tyr Val His Val Asn Gln Tyr Asp
725 730 735
Ile Val Leu Asp Val Leu Ile Val Asn Gln Thr Asn Asp Thr Leu Gln
740 745 750
Asn Cys Thr Leu Glu Leu Ala Thr Leu Gly Asp Leu Lys Leu Val Glu
755 760 765
Lys Pro Gln Pro Val Val Leu Ala Pro Lys Asp Phe Cys Asn Ile Lys
770 775 780
Ala Asn Val Lys Val Ala Ser Thr Glu Asn Gly Ile Ile Phe Gly Asn
785 790 795 800
Ile Val Tyr Asp Val Ile Gly Ala Gly Ser Asp Arg Asn Val Val Val
805 810 815
Leu Asn Asp Ile His Ile Asp Ile Met Asp Tyr Ile Val Pro Ala Ser
820 825 830
Cys Thr Asp Ser Glu Phe Met Arg Met Trp Ala Glu Phe Glu Trp Glu
835 840 845
Asn Lys Val Thr Val Asn Thr Pro Leu Thr Glu Leu Ser Glu Tyr Leu
850 855 860
Glu His Leu Leu Lys Ser Thr Asn Leu Lys Cys Leu Thr Ser Glu Lys
865 870 875 880
Ala Leu Ser Gly Gln Cys Gly Phe Met Ala Ala Asn Leu Tyr Ala Lys
885 890 895
Ser Ile Phe Gly Glu Asp Ala Leu Ala Asn Leu Ser Ile Glu Lys Pro
900 905 910
Phe Asn Lys Pro Asp Ala Pro Val Ser Gly His Ile Arg Ile Arg Ala
915 920 925
Lys Ser Gln Gly Met Ala Leu Ser Leu Gly Asp Lys Val Asn Met Thr
930 935 940
Gln Lys Ser Thr Gln His Lys Val Val Ala Ala
945 950 955
<210> 254
<211> 447
<212> PRT
<213> 玉米根萤叶甲属
<400> 254
Met Val Tyr Pro Asn Ser Pro Phe Lys Thr Thr Phe Asn Asn Lys Leu
1 5 10 15
Ile Thr Asn Leu Phe Leu Asp Asp Ser Val Thr Lys Thr Lys Thr Ser
20 25 30
Val Ser Asn Glu Glu Leu Leu Lys Ile Gln Gln Trp Leu Cys Ser Ala
35 40 45
Pro Lys Ile Thr Ser Ile Arg Val Asn Thr Ile Lys Thr Asn Thr Ala
50 55 60
Lys Val Leu Lys Val Leu Lys Thr Tyr Phe Ala Glu Asn Cys Asn Asp
65 70 75 80
Thr Pro Asn Val Tyr Ile His Pro Ser Phe Thr Asn Val Ile Ile Ile
85 90 95
Asp Ser Leu Asn Cys Pro Ala Gly Phe Lys Lys Phe Asp Lys Glu Ile
100 105 110
Ile Val Asp Thr Asp Cys Ala Ala Ala Ile Leu Arg Gly Ala His Ile
115 120 125
Phe Ala Pro Gly Val Leu Gly Met Val Ser Gly Cys Gln Ile Asn Glu
130 135 140
Asn Val Ser Ile Tyr Ala Asp Val Ala Lys Lys Cys Lys Lys Gly Leu
145 150 155 160
Gln Lys Ile Tyr Glu Asp Asp Phe Lys Ile Phe Ile Gly Asn Gly Ile
165 170 175
Val Lys Met Gln Arg His Gln Leu Phe Ala Thr Asp Asn Ile Ala Pro
180 185 190
Ser Gly Ile Ala Val Glu Val Thr Glu Thr Ile Ser Gly Cys Val Pro
195 200 205
Ile Ser Glu Ser Leu Leu Pro Val Gly Asp Ile Leu Leu Gln Asn Ile
210 215 220
Pro Ser Ile Val Cys Val His Asn Leu Asn Pro Lys Pro Gly Asp Val
225 230 235 240
Val Leu Asp Met Cys Ala Ser Pro Gly Asn Lys Thr Thr His Ile Ala
245 250 255
Glu Leu Met Gln Asn Lys Gly Ile Leu Ile Ala Ile Asp Lys Thr Pro
260 265 270
Lys Lys Val Ala Gln Leu Gln Lys Arg Cys Glu Asp Phe Gly Ala Lys
275 280 285
Val Tyr Ser Phe Gln Ala Asp Ser Thr Ala Ile Ile Ser Asp Thr Leu
290 295 300
Ser Ser Lys Asn Val Ile Asp Gly Pro Pro Phe Ala Pro Gln Ser Phe
305 310 315 320
Asp Lys Ile Leu Leu Asp Ala Pro Cys Ser Val Leu Gly Lys Arg Pro
325 330 335
Gln Leu Ala Asn Arg Ser Ser Glu Asn Glu Ile Lys Ser Phe Val Pro
340 345 350
Leu Gln Arg Lys Leu Phe Glu Asn Ala Ala Lys Leu Leu Lys Pro Gly
355 360 365
Gly Ile Met Val Tyr Ser Thr Cys Thr Ile Thr Leu Ser Glu Asn Glu
370 375 380
Gly Ile Val Ala Trp Ala Leu Lys Ser Phe Asn Phe Leu Glu Leu Val
385 390 395 400
Gln Pro Asp Leu Thr Leu Gly Glu Pro Gly Trp Leu Gly Thr Ser Leu
405 410 415
Ser Asp Glu Ala Arg Ser Phe Val Gln Arg Phe Gly Pro Asn Ser Glu
420 425 430
Val Asp Ser Val Gly Phe Phe Phe Ala Val Phe Lys Lys Lys Thr
435 440 445
<210> 255
<211> 1474
<212> PRT
<213> 玉米根萤叶甲属
<400> 255
Met Gly Arg Leu His Cys Leu Phe Cys Ile Phe Leu Cys Phe Thr Val
1 5 10 15
Ile Asn Thr Gln Thr Thr Asn Ile His Gly Phe Ser Glu Asn Ser Val
20 25 30
Asp Thr Phe Leu Ser Pro His Gly Lys Ser Ala Lys Phe Val His Gln
35 40 45
Asn His Lys Pro Lys Ile Glu Asn Cys Gln Asn Tyr Lys Pro Ser Val
50 55 60
Lys Glu Glu Gln Pro Gly Gly Thr Tyr Val Thr Thr Val Thr Ala Ile
65 70 75 80
Asp Asp Asp Pro Arg Glu Gly Gly Gly Thr Ile Ser Tyr Lys Leu Ile
85 90 95
His Arg Glu Gly Glu His Val Leu Phe Asp Ile Asp Asn Val Thr Gly
100 105 110
Val Leu Thr Thr Ile Gln Pro Phe Asp Arg Asp Glu Pro Val Arg Gln
115 120 125
Lys Glu Leu Tyr Val Thr Val Gln Ala Ser Asp Asn Gly Arg Pro Pro
130 135 140
Leu Ala Asp Val Cys Thr Phe Thr Val Thr Ile Thr Asp Ile Asn Asp
145 150 155 160
Asn Ala Pro Gln Leu Asp Lys Leu Lys Tyr Asp Ala Gln Val Ser Glu
165 170 175
Asp Leu Lys Val Gly Ser Glu Val Met Arg Val Phe Ala Tyr Asp Ile
180 185 190
Asp Asp Gly Glu Asn Ser Arg Leu Ser Tyr Asn Phe Ser Asn Glu Asn
195 200 205
Ala Gln Phe Thr Gln Tyr Phe Arg Ile Asp Arg Asp Thr Gly Val Val
210 215 220
Tyr Leu Lys Glu Ala Leu Thr Asp Lys Lys Asn Thr Arg Phe Asn Ser
225 230 235 240
Ala Val Tyr Val Ala Asp Asn Gly Val Asn Asp Gln Glu Gly Gln Lys
245 250 255
Asp Ser Thr Ala Lys Ile Ser Ile Thr Val Val Gly Ser Asp Lys Gln
260 265 270
Pro Pro Arg Phe Thr Gln Lys Met Pro Asp Gly Ile Leu Glu Ile Pro
275 280 285
Glu Asp Phe Lys Asp Phe Ser Lys His Ile Val Thr Val Glu Ala Thr
290 295 300
Ser Asn Ile Ala Asp Pro Gln Leu Ala Phe Glu Leu Val Lys Gly Lys
305 310 315 320
Thr Tyr Gln Thr Asn Lys Asp Gln Thr Phe Leu Leu Glu Ala Glu Gly
325 330 335
Asn Lys Ala His Ile Lys Leu Val Arg Pro Leu Asp Tyr Glu Thr Val
340 345 350
Thr Glu Tyr Thr Leu Thr Ile Arg Val Lys Asn Lys Asp Leu Met Asp
355 360 365
Ser Ser Ile Asn Ile Pro Ile Lys Val Leu Asp Val Asn Asp Glu Ile
370 375 380
Pro Asn Phe Leu Glu Phe Leu Lys Gly Ser Val Val Glu Asn Asp Lys
385 390 395 400
Pro Gly Ala Gln Ala Ile Gln Val Arg Ala Ile Asp Lys Asp Gly Thr
405 410 415
Ala Ala Asn Asn Ile Val Ser Tyr Glu Leu Val Asp Asn Thr Asp Leu
420 425 430
Phe Ala Ile Asn Arg Ser Thr Gly Val Ile Thr Ser Arg Val Glu Phe
435 440 445
Asp Arg Glu Thr Val Pro Leu Tyr His Val Asn Val Lys Ala Tyr Asp
450 455 460
Asn Ser Pro Ser Ala Leu Tyr Asn Thr Thr Leu Pro Asn Ile Val Ile
465 470 475 480
Gln Thr Phe Gln Ile Ser Ile Glu Asp Gln Asn Asp Asn Lys Pro Val
485 490 495
Phe Thr His Pro Ile Tyr Gln Phe Ser Asn Ile Thr Glu Leu Ala Asp
500 505 510
Lys Ser Ser Ile Val Gly Glu Val Lys Ala Leu Asp Asn Asp Thr Ala
515 520 525
Ser Val Ile Ser Tyr Ser Ile Thr Asn Gly Asn Ile Asp Asp Ala Phe
530 535 540
Met Ile Glu Asn Ser Thr Gly Arg Ile Arg Val Asn Gly Lys Leu Asp
545 550 555 560
Tyr Glu Lys Ile Glu Gln Tyr Asn Leu Thr Val Arg Ala Phe Asp Gly
565 570 575
Ala Phe Glu Asp Phe Ala Ile Val Leu Ile Ser Ile Leu Asn Glu Asn
580 585 590
Asp Glu Pro Pro Val Phe Asp Asp Tyr Ile Arg Glu Ile Gln Ile Lys
595 600 605
Glu Glu Glu Pro Met Ile Ser Gly Cys Val Val Arg Val Thr Ala His
610 615 620
Asp Pro Asp Ile Lys Asp Arg His Ala Asp Gln His Ile Val Tyr Glu
625 630 635 640
Val Ala Lys Glu Gln Lys Asp Phe Leu Thr Val Ser Ala Asp Gly Cys
645 650 655
Val Gln Val Thr Lys Pro Leu Asp Arg Asp Pro Pro Phe Gly Ser Pro
660 665 670
Thr Arg Gln Val Phe Ile Tyr Ala Arg Asp Asn Asp Gly Gly Thr Asn
675 680 685
Ser Leu Leu Ala Thr Ala Glu Ile Glu Ile Ile Leu Ile Asp Ile Asn
690 695 700
Asp Asn Ala Pro Phe Leu Asn Val Thr Glu Ile Val Tyr Tyr Glu Asn
705 710 715 720
Gln Asp Pro Gly Phe Ile Gly Asn Leu Ser Ala Asp Asp Tyr Asp Gly
725 730 735
Pro Asp Asn Gly Pro Pro Phe Ala Phe Arg Leu Ser Asp Thr Ala Ser
740 745 750
Asp Ser Ile Arg Ser Lys Phe Ser Ile Ile Gly Asn Gln Leu Phe Ala
755 760 765
Leu Glu Met Phe Asp Arg Glu Glu Gln Lys Tyr Tyr Asp Ile Ala Ile
770 775 780
Asp Ile Thr Asp Ser Gly Val Pro Pro Leu Thr Gly Thr Ser Ile Leu
785 790 795 800
Arg Val Ile Ile Gly Asp Val Asn Asp Asn Pro Ala Thr Asp Gly Asn
805 810 815
Ser Thr Ile Phe Val Tyr Lys Tyr Val Asn Gly Pro Glu Asn Phe Met
820 825 830
Glu Ile Gly Arg Val Tyr Val Thr Asp Leu Asp Asp Trp Asp Leu Asn
835 840 845
Asp Lys Val Phe Val Gln Glu Asp Asn Phe Asp Glu Phe Val Leu Asn
850 855 860
Gln His Asn Asn Gly Met Ile Leu Met Lys Pro Thr Thr Ala Glu Gly
865 870 875 880
Thr Tyr Glu Val His Tyr Arg Val Thr Glu Thr His Glu Pro Thr Ile
885 890 895
His Glu His Thr Val Asn Ala Ile Val Thr Ile Thr Val Lys Val Leu
900 905 910
Pro Glu Glu Ala Val Val Lys Ser Gly Ser Ile Arg Leu Arg Gly Thr
915 920 925
Thr Lys Glu Glu Phe Ile Glu Asn Ser Leu Asn Gly Lys Ser Lys Arg
930 935 940
Asp Ile Leu His Gln Glu Leu Ser Lys Ile Leu Asn Thr Ser Leu Ala
945 950 955 960
Asn Val Asp Val Phe Thr Val Leu Asn Ser Pro His Gln Asn Ser Ser
965 970 975
Phe Val Asp Val Arg Phe Ser Ala His Gly Ser Pro Tyr Tyr Ala Pro
980 985 990
Glu Lys Leu Glu Asn Lys Val Thr Asp His Gln Met Glu Leu Glu Gln
995 1000 1005
Lys Leu Asp Val Glu Phe Tyr Met Ile Asn Val Asn Glu Cys Leu
1010 1015 1020
Asn Glu Thr Thr Cys Gly Ala Glu Asn Ser Cys Thr Asn Lys Leu
1025 1030 1035
Asn Ile Thr Arg Glu Pro Ala Val Val Phe Thr Asn Arg Thr Ser
1040 1045 1050
Phe Val Gly Val Asn Ala Phe Ile Asp Pro Val Cys Ala Ala Leu
1055 1060 1065
Pro Arg Asp Val Met Glu Cys Phe Asn Gly Gly Val Leu Ile Glu
1070 1075 1080
Asn Thr Ala Cys Asn Cys Pro Ala Gly Phe Glu Gly Pro His Cys
1085 1090 1095
Glu Ile Leu Ala Ile Gly Phe Thr Gly Thr Gly Trp Ala Met Tyr
1100 1105 1110
Pro Ser Phe Asp Ala Thr Asn Arg Thr Glu Ile Ile Leu His Ile
1115 1120 1125
Leu Ser Gln Thr Asp Asn Gly Leu Ile Phe Tyr Asn Gly Pro Leu
1130 1135 1140
Asn Ile Arg Gln Thr Ser Leu Ser Lys Asp Tyr Ile Ser Leu Glu
1145 1150 1155
Leu Lys Asp Gly Tyr Pro Leu Leu Gln Ile Cys Thr Gly Ser Ser
1160 1165 1170
Thr Gln Glu Ile Tyr Leu Lys Glu Arg Ile His Lys Leu Ser Asp
1175 1180 1185
Gly Ser Leu His Lys Ile Lys Ile Gly Ser Gly Phe Asp Asp Ile
1190 1195 1200
Ser Leu Glu Val Asp Asp Cys Gly Thr Thr Cys Ser Ile Trp Thr
1205 1210 1215
Asn Lys Leu His Lys Gly Val Ile Arg Ala Asn Gly Pro Leu Gln
1220 1225 1230
Leu Gly Gly Met Lys Asn Arg Phe Thr Asp Gln Glu Phe Lys Arg
1235 1240 1245
Ile Trp Asp His Leu Pro Pro Thr Ala Thr Arg Phe Ser Gly Cys
1250 1255 1260
Ile Arg Asn Leu Thr Tyr Asn Glu Phe Tyr Tyr Asn Leu Gly Ala
1265 1270 1275
Pro Ser Asp Ala Phe Gln Ala Tyr Pro Asp Cys Asn Tyr Ala Val
1280 1285 1290
Met Gln Ala Val Thr Phe Gly Ile Asp Ser Asn Phe Leu Val Ala
1295 1300 1305
Ile Leu Val Cys Val Ala Ile Leu Ile Ile Leu Leu Leu Ala Val
1310 1315 1320
Val Val His Arg Arg Lys His Asp Asn Phe Asn Glu Lys Glu Ile
1325 1330 1335
Asp Asp Thr Arg Glu Asn Ile Ile Asn Tyr Glu Asp Glu Gly Gly
1340 1345 1350
Gly Glu Cys Asp Thr Asn Tyr Asp Leu Ser Val Phe His Gln Asn
1355 1360 1365
Asn Ile Val Asp Glu Lys Pro Leu Met Arg Asp Asn Pro Asp Val
1370 1375 1380
Pro Ala Asp Ile Ser Gly Phe Leu Asp Asn Lys Lys Asp Asn Cys
1385 1390 1395
Asp Lys Asp Pro Asp Asn Leu Pro Tyr Asp Asp Val Arg His Tyr
1400 1405 1410
Ala Tyr Glu Gly Asp Gly Asn Ser Thr Gly Ser Leu Ser Ser Leu
1415 1420 1425
Ala Ser Cys Thr Asp Glu Gly Asp Leu Lys Phe Asn Tyr Leu Ser
1430 1435 1440
Ser Phe Gly Pro Arg Phe Arg Lys Leu Ala Asp Met Tyr Gly Glu
1445 1450 1455
Asp Pro Ser Asp Glu Asp Ser His Asp Gly Asn Glu Glu Ser Trp
1460 1465 1470
Cys
<210> 256
<211> 96
<212> PRT
<213> 玉米根萤叶甲属
<400> 256
Met Pro Phe Cys Gly Pro Lys Leu Ser Leu Cys Gly Leu Ile Ile Ser
1 5 10 15
Ala Trp Gly Ile Ile Gln Leu Gly Phe Met Gly Val Phe Tyr Tyr Ile
20 25 30
Gly Ala Val Ala Leu Ala Glu Asp Ile Pro Glu Val Glu Phe Lys Gly
35 40 45
Asp Leu Asp Lys Phe Tyr Ser Asp Val Asn Thr Gly Phe Thr Gln Asn
50 55 60
Ala Tyr Asn Cys Trp Ile Ala Ala Leu Leu Tyr Leu Ile Thr Leu Ala
65 70 75 80
Val Ser Ala His Gln Phe Trp Ala Asn Asn Arg Ser Ser Leu Asn Val
85 90 95
<210> 257
<211> 179
<212> PRT
<213> 玉米根萤叶甲属
<400> 257
Met Gly Leu Thr Ile Ser Ala Val Phe Asn Arg Leu Phe Ser Lys Lys
1 5 10 15
Pro Met Arg Ile Leu Met Val Gly Leu Asp Ala Ala Gly Lys Thr Thr
20 25 30
Ile Leu Tyr Lys Leu Lys Leu Gly Glu Ile Val Thr Thr Ile Pro Thr
35 40 45
Ile Gly Phe Asn Val Glu Thr Val Glu Tyr Lys Asn Ile Ser Phe Thr
50 55 60
Val Trp Asp Val Gly Gly Gln Thr Arg Ile Arg Lys Leu Trp Arg His
65 70 75 80
Tyr Phe Ala Asn Thr Asp Gly Leu Ile Phe Val Val Asp Ser Asn Asp
85 90 95
Arg Asp Arg Ile Ala Glu Ala Glu Glu Glu Leu His Asn Met Leu Gly
100 105 110
Glu Asp Asp Leu Arg Asp Cys Ile Leu Leu Ile Phe Ala Asn Lys Gln
115 120 125
Asp Leu Pro Asn Ser Met Ser Thr Ala Glu Leu Thr Asp Lys Leu Lys
130 135 140
Leu His Thr Leu Lys Asn Arg Arg Trp Tyr Ile Gln Ala Thr Cys Ala
145 150 155 160
Thr Gln Gly Asn Gly Leu Tyr Glu Gly Leu Asp Trp Leu Ser Asn Glu
165 170 175
Leu Ala Lys
<210> 258
<211> 205
<212> PRT
<213> 玉米根萤叶甲属
<400> 258
Met Arg Tyr Thr Leu Ser Tyr Ile Gly Ala Thr Leu Ala Ser Thr Val
1 5 10 15
Thr Leu Ile Phe Ala Leu Tyr Tyr Cys Leu Thr Gly Lys Gly Glu Gln
20 25 30
Val Ser Leu Ala Trp Leu Leu Leu Asn Val Ser Pro His Met Trp Ala
35 40 45
Gly Leu Gly Ile Gly Leu Ala Val Ser Leu Ser Val Val Gly Ala Ala
50 55 60
Ala Gly Ile His Thr Thr Gly Val Ser Ile Val Gly Ala Gly Val Lys
65 70 75 80
Ala Pro Arg Ile Lys Thr Lys Asn Leu Ile Ser Ile Ile Phe Cys Glu
85 90 95
Ala Val Ala Ile Tyr Gly Leu Ile Met Ala Ile Val Leu Cys Gly Ser
100 105 110
Trp Lys Asn Phe Asp Val Asp Leu Phe Asn Leu Lys Thr His Asn Phe
115 120 125
Ala Gln Asn His Tyr Gly Ser His Val Ile Phe Gly Ser Gly Leu Thr
130 135 140
Val Gly Phe Val Asn Leu Leu Cys Gly Phe Cys Val Gly Val Val Gly
145 150 155 160
Ser Gly Ala Ala Ile Ser Asp Ala Ala Asn Ser Ser Leu Phe Val Lys
165 170 175
Ile Leu Ile Ile Glu Ile Phe Gly Ser Ala Ile Gly Leu Phe Gly Leu
180 185 190
Ile Val Gly Val Tyr Leu Thr Ser Arg Gly Ser Met Val
195 200 205
<210> 259
<211> 1897
<212> PRT
<213> 玉米根萤叶甲属
<400> 259
Met Ala Thr Asn Asp Ser Lys Ala Pro Leu Arg Thr Val Lys Arg Val
1 5 10 15
Gln Phe Gly Ile Leu Ser Pro Asp Glu Ile Arg Arg Met Ser Val Thr
20 25 30
Glu Gly Gly Ile Arg Phe Pro Glu Thr Met Glu Ala Gly Arg Pro Lys
35 40 45
Leu Cys Gly Leu Met Asp Pro Arg Gln Gly Val Ile Asp Arg Ser Ser
50 55 60
Arg Cys Gln Thr Cys Ala Gly Asn Met Thr Glu Cys Pro Gly His Phe
65 70 75 80
Gly His Ile Glu Leu Ala Lys Pro Val Phe His Val Gly Phe Val Thr
85 90 95
Lys Thr Ile Lys Ile Leu Arg Cys Val Cys Phe Phe Cys Ser Lys Leu
100 105 110
Leu Val Ser Pro Asn Asn Pro Lys Ile Lys Glu Val Val Met Lys Ser
115 120 125
Lys Gly Gln Pro Arg Lys Arg Leu Ala Phe Val Tyr Asp Leu Cys Lys
130 135 140
Gly Lys Asn Ile Cys Glu Gly Gly Asp Glu Met Asp Val Gly Lys Glu
145 150 155 160
Ser Glu Asp Pro Asn Lys Lys Ala Gly His Gly Gly Cys Gly Arg Tyr
165 170 175
Gln Pro Asn Ile Arg Arg Ala Gly Leu Asp Leu Thr Ala Glu Trp Lys
180 185 190
His Val Asn Glu Asp Thr Gln Glu Lys Lys Ile Ala Leu Ser Ala Glu
195 200 205
Arg Val Trp Glu Ile Leu Lys His Ile Thr Asp Glu Glu Cys Phe Ile
210 215 220
Leu Gly Met Asp Pro Lys Phe Ala Arg Pro Asp Trp Met Ile Val Thr
225 230 235 240
Val Leu Pro Val Pro Pro Leu Ala Val Arg Pro Ala Val Val Met His
245 250 255
Gly Ser Ala Arg Asn Gln Asp Asp Ile Thr His Lys Leu Ala Asp Ile
260 265 270
Ile Lys Ala Asn Asn Glu Leu Gln Lys Asn Glu Ser Ala Gly Ala Ala
275 280 285
Ala His Ile Ile Thr Glu Asn Ile Lys Met Leu Gln Phe His Val Ala
290 295 300
Thr Leu Val Asp Asn Asp Met Pro Gly Met Pro Arg Ala Met Gln Lys
305 310 315 320
Ser Gly Lys Pro Leu Lys Ala Ile Lys Ala Arg Leu Lys Gly Lys Glu
325 330 335
Gly Arg Ile Arg Gly Asn Leu Met Gly Lys Arg Val Asp Phe Ser Ala
340 345 350
Arg Thr Val Ile Thr Pro Asp Pro Asn Leu Arg Ile Asp Gln Val Gly
355 360 365
Val Pro Arg Ser Ile Ala Gln Asn Met Thr Phe Pro Glu Ile Val Thr
370 375 380
Pro Phe Asn Phe Asp Lys Met Leu Glu Leu Val Gln Arg Gly Asn Ser
385 390 395 400
Gln Tyr Pro Gly Ala Lys Tyr Ile Ile Arg Asp Asn Gly Glu Arg Ile
405 410 415
Asp Leu Arg Phe His Pro Lys Pro Ser Asp Leu His Leu Gln Cys Gly
420 425 430
Tyr Lys Val Glu Arg His Ile Arg Asp Gly Asp Leu Val Ile Phe Asn
435 440 445
Arg Gln Pro Thr Leu His Lys Met Ser Met Met Gly His Arg Val Lys
450 455 460
Val Leu Pro Trp Ser Thr Phe Arg Met Asn Leu Ser Cys Thr Ser Pro
465 470 475 480
Tyr Asn Ala Asp Phe Asp Gly Asp Glu Met Asn Leu His Val Pro Gln
485 490 495
Ser Met Glu Thr Arg Ala Glu Val Glu Asn Leu His Ile Thr Pro Arg
500 505 510
Gln Ile Ile Thr Pro Gln Ala Asn Gln Pro Val Met Gly Ile Val Gln
515 520 525
Asp Thr Leu Thr Ala Val Arg Lys Met Thr Lys Arg Asp Val Phe Ile
530 535 540
Glu Lys Glu Gln Met Met Asn Ile Leu Met Phe Leu Pro Ile Trp Asp
545 550 555 560
Gly Lys Met Pro Arg Pro Ala Ile Leu Lys Pro Lys Pro Leu Trp Thr
565 570 575
Gly Lys Gln Ile Phe Ser Leu Ile Ile Pro Gly Asn Val Asn Met Ile
580 585 590
Arg Thr His Ser Thr His Pro Asp Asp Glu Asp Asp Gly Pro Tyr Lys
595 600 605
Trp Ile Ser Pro Gly Asp Thr Lys Val Met Val Glu His Gly Glu Leu
610 615 620
Val Met Gly Ile Leu Cys Lys Lys Ser Leu Gly Thr Ser Ala Gly Ser
625 630 635 640
Leu Leu His Ile Cys Met Leu Glu Leu Gly His Glu Val Cys Gly Arg
645 650 655
Phe Tyr Gly Asn Ile Gln Thr Val Ile Asn Asn Trp Leu Leu Leu Glu
660 665 670
Gly His Ser Ile Gly Ile Gly Asp Thr Ile Ala Asp Pro Gln Thr Tyr
675 680 685
Thr Glu Ile Gln Arg Ala Ile Arg Lys Ala Lys Glu Asp Val Ile Glu
690 695 700
Val Ile Gln Lys Ala His Asn Met Glu Leu Glu Pro Thr Pro Gly Asn
705 710 715 720
Thr Leu Arg Gln Thr Phe Glu Asn Gln Val Asn Arg Ile Leu Asn Asp
725 730 735
Ala Arg Asp Lys Thr Gly Gly Ser Ala Lys Lys Ser Leu Thr Glu Tyr
740 745 750
Asn Asn Leu Lys Ala Met Val Val Ser Gly Ser Lys Gly Ser Asn Ile
755 760 765
Asn Ile Ser Gln Val Ile Ala Cys Val Gly Gln Gln Asn Val Glu Gly
770 775 780
Lys Arg Ile Pro Phe Gly Phe Arg Lys Arg Thr Leu Pro His Phe Ile
785 790 795 800
Lys Asp Asp Tyr Gly Pro Glu Ser Arg Gly Phe Val Glu Asn Ser Tyr
805 810 815
Leu Ala Gly Leu Thr Pro Ser Glu Phe Tyr Phe His Ala Met Gly Gly
820 825 830
Arg Glu Gly Leu Ile Asp Thr Ala Val Lys Thr Ala Glu Thr Gly Tyr
835 840 845
Ile Gln Arg Arg Leu Ile Lys Ala Met Glu Ser Val Met Val His Tyr
850 855 860
Asp Gly Thr Val Arg Asn Ser Val Gly Gln Leu Ile Gln Leu Arg Tyr
865 870 875 880
Gly Glu Asp Gly Leu Cys Gly Glu Met Val Glu Phe Gln Tyr Leu Ala
885 890 895
Thr Val Lys Leu Ser Asn Lys Ala Phe Glu Arg Lys Phe Arg Phe Asp
900 905 910
Pro Ser Asn Glu Arg Tyr Leu Arg Arg Val Phe Asn Glu Glu Val Ile
915 920 925
Lys Gln Leu Met Gly Ser Gly Glu Val Ile Ser Glu Leu Glu Arg Glu
930 935 940
Trp Glu Gln Leu Gln Lys Asp Arg Glu Ala Leu Arg Gln Ile Phe Pro
945 950 955 960
Ser Gly Glu Ser Lys Val Val Leu Pro Cys Asn Leu Gln Arg Met Ile
965 970 975
Trp Asn Val Gln Lys Ile Phe His Ile Asn Lys Arg Ala Pro Thr Asp
980 985 990
Leu Ser Pro Leu Arg Val Ile Gln Gly Val Arg Glu Leu Leu Arg Lys
995 1000 1005
Cys Val Ile Val Ala Gly Glu Asp Arg Leu Ser Lys Gln Ala Asn
1010 1015 1020
Glu Asn Ala Thr Leu Leu Phe Gln Cys Leu Val Arg Ser Thr Leu
1025 1030 1035
Cys Thr Lys Cys Val Ser Glu Glu Phe Arg Leu Ser Thr Glu Ala
1040 1045 1050
Phe Glu Trp Leu Ile Gly Glu Ile Glu Thr Arg Phe Gln Gln Ala
1055 1060 1065
Gln Ala Asn Pro Gly Glu Met Val Gly Ala Leu Ala Ala Gln Ser
1070 1075 1080
Leu Gly Glu Pro Ala Thr Gln Met Thr Leu Asn Thr Phe His Phe
1085 1090 1095
Ala Gly Val Ser Ser Lys Asn Val Thr Leu Gly Val Pro Arg Leu
1100 1105 1110
Lys Glu Ile Ile Asn Ile Ser Lys Lys Pro Lys Ala Pro Ser Leu
1115 1120 1125
Thr Val Phe Leu Thr Gly Ala Ala Ala Arg Asp Ala Glu Lys Ala
1130 1135 1140
Lys Asn Val Leu Cys Arg Leu Glu His Thr Thr Leu Arg Lys Val
1145 1150 1155
Thr Ala Asn Thr Ala Ile Tyr Tyr Asp Pro Asp Pro Gln Asn Thr
1160 1165 1170
Val Ile Pro Glu Asp Gln Glu Phe Val Asn Val Tyr Tyr Glu Met
1175 1180 1185
Pro Asp Phe Asp Pro Thr Arg Ile Ser Pro Trp Leu Leu Arg Ile
1190 1195 1200
Glu Leu Asp Arg Lys Arg Met Thr Asp Lys Lys Leu Thr Met Glu
1205 1210 1215
Gln Ile Ala Glu Lys Ile Asn Ala Gly Phe Gly Asp Asp Leu Asn
1220 1225 1230
Cys Ile Phe Asn Asp Asp Asn Ala Glu Lys Leu Val Leu Arg Ile
1235 1240 1245
Arg Ile Met Asn Ser Asp Asp Gly Lys Phe Gly Glu Gly Ala Asp
1250 1255 1260
Glu Asp Val Asp Lys Met Asp Asp Asp Met Phe Leu Arg Cys Ile
1265 1270 1275
Glu Ala Asn Met Leu Ser Asp Met Thr Leu Gln Gly Ile Glu Ala
1280 1285 1290
Ile Ser Lys Val Tyr Met His Leu Pro Gln Thr Asp Ser Lys Lys
1295 1300 1305
Arg Ile Val Ile Thr Glu Thr Gly Glu Phe Lys Ala Ile Ala Glu
1310 1315 1320
Trp Leu Leu Glu Thr Asp Gly Thr Ser Met Met Lys Val Leu Ser
1325 1330 1335
Glu Arg Asp Val Asp Pro Val Arg Thr Phe Ser Asn Asp Ile Cys
1340 1345 1350
Glu Ile Phe Ser Val Leu Gly Ile Glu Ala Val Arg Lys Ser Val
1355 1360 1365
Glu Lys Glu Met Asn Ala Val Leu Ser Phe Tyr Gly Leu Tyr Val
1370 1375 1380
Asn Tyr Arg His Leu Ala Leu Leu Cys Asp Val Met Thr Ala Lys
1385 1390 1395
Gly His Leu Met Ala Ile Thr Arg His Gly Ile Asn Arg Gln Asp
1400 1405 1410
Thr Gly Ala Leu Met Arg Cys Ser Phe Glu Glu Thr Val Asp Val
1415 1420 1425
Leu Met Asp Ala Ala Ser His Ala Glu Val Asp Pro Met Arg Gly
1430 1435 1440
Val Ser Glu Asn Ile Ile Leu Gly Gln Leu Pro Arg Met Gly Thr
1445 1450 1455
Gly Cys Phe Asp Leu Leu Leu Asp Ala Glu Lys Cys Lys Met Gly
1460 1465 1470
Ile Ala Ile Pro Gln Ala His Ser Ser Asp Leu Met Ala Ser Gly
1475 1480 1485
Met Phe Phe Gly Leu Ala Ala Thr Pro Ser Ser Met Ser Pro Gly
1490 1495 1500
Gly Ala Met Thr Pro Trp Asn Gln Ala Ala Thr Pro Tyr Val Gly
1505 1510 1515
Ser Ile Trp Ser Pro Gln Asn Leu Met Gly Ser Gly Met Thr Pro
1520 1525 1530
Gly Gly Ala Ala Phe Ser Pro Ser Ala Ala Ser Asp Ala Ser Gly
1535 1540 1545
Met Ser Pro Ala Tyr Gly Gly Trp Ser Pro Thr Pro Gln Ser Pro
1550 1555 1560
Ala Met Ser Pro Tyr Met Ala Ser Pro His Gly Gln Ser Pro Ser
1565 1570 1575
Tyr Ser Pro Ser Ser Pro Ala Phe Gln Pro Thr Ser Pro Ser Met
1580 1585 1590
Thr Pro Thr Ser Pro Gly Tyr Ser Pro Ser Ser Pro Gly Tyr Ser
1595 1600 1605
Pro Thr Ser Leu Asn Tyr Ser Pro Thr Ser Pro Ser Tyr Ser Pro
1610 1615 1620
Thr Ser Gln Ser Tyr Ser Pro Thr Ser Pro Ser Tyr Ser Pro Thr
1625 1630 1635
Ser Pro Asn Tyr Ser Pro Thr Ser Pro Ser Tyr Ser Pro Thr Ser
1640 1645 1650
Pro Asn Tyr Ser Pro Thr Ser Pro Asn Tyr Ser Pro Thr Ser Pro
1655 1660 1665
Ser Tyr Pro Ser Thr Ser Pro Gly Tyr Ser Pro Thr Ser Arg Ser
1670 1675 1680
Tyr Ser Pro Thr Ser Pro Ser Tyr Ser Gly Thr Ser Pro Ser Tyr
1685 1690 1695
Ser Pro Thr Ser Pro Ser Tyr Ser Pro Thr Ser Pro Ser Tyr Ser
1700 1705 1710
Pro Ser Ser Pro Asn Tyr Ser Pro Thr Ser Pro Asn Tyr Ser Pro
1715 1720 1725
Thr Ser Pro Asn Tyr Ser Pro Ser Ser Pro Arg Tyr Thr Pro Gly
1730 1735 1740
Ser Pro Ser Phe Ser Pro Ser Ser Asn Ser Tyr Ser Pro Thr Ser
1745 1750 1755
Pro Gln Tyr Ser Pro Thr Ser Pro Ser Tyr Ser Pro Ser Ser Pro
1760 1765 1770
Lys Tyr Ser Pro Thr Ser Pro Asn Tyr Ser Pro Thr Ser Pro Ser
1775 1780 1785
Phe Ser Gly Gly Ser Pro Gln Tyr Ser Pro Thr Ser Pro Lys Tyr
1790 1795 1800
Ser Pro Thr Ser Pro Asn Tyr Thr Leu Ser Ser Pro Gln His Thr
1805 1810 1815
Pro Thr Gly Ser Ser Arg Tyr Ser Pro Ser Thr Ser Ser Tyr Ser
1820 1825 1830
Pro Asn Ser Pro Asn Tyr Ser Pro Thr Ser Pro Gln Tyr Ser Ile
1835 1840 1845
His Ser Thr Lys Tyr Ser Pro Ala Ser Pro Thr Phe Thr Pro Thr
1850 1855 1860
Ser Pro Ser Phe Ser Pro Ala Ser Pro Ala Tyr Ser Pro Gln Pro
1865 1870 1875
Met Tyr Ser Pro Ser Ser Pro Asn Tyr Ser Pro Thr Ser Pro Ser
1880 1885 1890
Gln Asp Thr Asp
1895
<210> 260
<211> 291
<212> PRT
<213> 玉米根萤叶甲属
<400> 260
Met Ser Ser Asn Ile Gln Lys Ala Gln Gln Leu Met Ala Asp Ala Glu
1 5 10 15
Lys Lys Val Thr Ser Arg Gly Phe Phe Gly Ser Leu Phe Gly Gly Ser
20 25 30
Ser Arg Ile Glu Asp Ala Val Glu Cys Tyr Thr Arg Ala Ala Asn Leu
35 40 45
Phe Lys Met Ala Lys Ser Trp Asp Ala Ala Gly Lys Ala Phe Cys Glu
50 55 60
Ala Ala Asn Leu His Ser Arg Thr Gly Ala Arg His Asp Ala Ala Thr
65 70 75 80
Asn Tyr Ile Asp Ala Ala Asn Cys Tyr Lys Lys Ala Asp Val Phe Glu
85 90 95
Ala Val Asn Cys Phe Ile Lys Ala Ile Asp Ile Tyr Thr Glu Met Gly
100 105 110
Arg Phe Thr Met Ala Ala Lys His His Gln Thr Ile Ala Glu Met Tyr
115 120 125
Glu Thr Asp Ala Val Asp Ile Glu Arg Ala Val Gln His Tyr Glu Gln
130 135 140
Ala Ala Asp Tyr Phe Arg Gly Glu Glu Ser Asn Ala Ser Ala Asn Lys
145 150 155 160
Cys Leu Leu Lys Val Ala Gln Tyr Ala Ala Gln Leu Glu Asn Tyr Glu
165 170 175
Lys Ala Val Gly Ile Tyr Gln Glu Val Ala Tyr Ala Ala Leu Glu Ser
180 185 190
Ser Leu Leu Lys Tyr Ser Ala Lys Glu Tyr Leu Phe Arg Ala Ala Leu
195 200 205
Cys His Leu Cys Val Asp Val Leu Asn Ala Gln His Ala Ile Glu Ser
210 215 220
Tyr Ile Ser Arg Tyr Pro Ala Phe Gln Asp Ser Arg Glu Tyr Lys Leu
225 230 235 240
Leu Lys Thr Leu Ile Glu Asn Ile Glu Glu Gln Asn Val Asp Gly Tyr
245 250 255
Thr Glu Ala Val Lys Asp Tyr Asp Ser Ile Ser Arg Leu Asp Gln Trp
260 265 270
Tyr Thr Thr Ile Leu Leu Arg Ile Lys Lys Gln Val Ser Glu Ser Pro
275 280 285
Asp Leu Arg
290
<210> 261
<211> 202
<212> PRT
<213> 玉米根萤叶甲属
<400> 261
Met Asn Pro Glu Tyr Asp Tyr Leu Phe Lys Leu Leu Leu Ile Gly Asp
1 5 10 15
Ser Gly Val Gly Lys Ser Cys Leu Leu Leu Arg Phe Ala Asp Asp Thr
20 25 30
Tyr Thr Glu Ser Tyr Ile Ser Thr Ile Gly Val Asp Phe Lys Ile Arg
35 40 45
Thr Ile Asp Leu Asp Gly Lys Thr Ile Lys Leu Gln Ile Trp Asp Thr
50 55 60
Ala Gly Gln Glu Arg Phe Arg Thr Ile Thr Ser Ser Tyr Tyr Arg Gly
65 70 75 80
Ala His Gly Ile Ile Val Val Tyr Asp Cys Thr Asp Gln Asp Ser Phe
85 90 95
Asn Asn Val Lys Gln Trp Leu Glu Glu Ile Asp Arg Tyr Ala Cys Asp
100 105 110
Asn Val Asn Lys Leu Leu Val Gly Asn Lys Ser Asp Leu Thr Thr Lys
115 120 125
Lys Val Val Asp Phe Thr Thr Ala Lys Glu Tyr Ala Asp Gln Leu Gly
130 135 140
Ile Pro Phe Leu Glu Thr Ser Ala Lys Asn Ala Thr Asn Val Glu Gln
145 150 155 160
Ala Phe Met Thr Met Ala Ala Glu Ile Lys Asn Arg Val Gly Pro Pro
165 170 175
Ser Ser Ala Val Asp Gln Gly Asn Lys Val Arg Phe Asp Gln Ser Arg
180 185 190
Pro Val Glu Thr Thr Lys Ser Gly Cys Cys
195 200
<210> 262
<211> 262
<212> PRT
<213> 玉米根萤叶甲属
<400> 262
Met Ala Asp Ala Asp Asp Leu Leu Asp Tyr Glu Asp Glu Glu Gln Thr
1 5 10 15
Glu Gln Thr Ala Thr Glu Thr Ala Thr Thr Glu Val Gln Lys Lys Gly
20 25 30
Val Lys Gly Thr Tyr Val Ser Ile His Ser Ser Gly Phe Arg Asp Phe
35 40 45
Leu Leu Lys Pro Ala Ile Leu Arg Ala Ile Val Asp Cys Gly Phe Glu
50 55 60
His Pro Ser Glu Val Gln His Glu Cys Ile Pro Gln Ala Val Ile Gly
65 70 75 80
Met Asp Ile Leu Cys Gln Ala Lys Ser Gly Met Gly Lys Thr Ala Val
85 90 95
Phe Val Leu Ala Thr Leu Gln Val Ile Asp Pro Thr Glu Asn Val Val
100 105 110
Tyr Val Leu Val Met Cys His Thr Arg Glu Leu Ala Phe Gln Ile Ser
115 120 125
Lys Glu Tyr Glu Arg Phe Ser Lys Tyr Met Pro Asn Ile Lys Val Gly
130 135 140
Val Phe Phe Gly Gly Leu Pro Ile Gln Lys Asp Glu Glu Thr Leu Lys
145 150 155 160
Asn Asn Cys Pro His Ile Val Val Gly Thr Pro Gly Arg Ile Leu Ala
165 170 175
Leu Val Arg Ser Lys Lys Leu Asn Leu Lys His Leu Lys His Phe Ile
180 185 190
Leu Asp Glu Cys Asp Lys Met Leu Glu Leu Leu Asp Met Arg Arg Asp
195 200 205
Val Gln Glu Ile Tyr Arg Asn Thr Pro His Glu Lys Gln Val Met Met
210 215 220
Phe Ser Ala Thr Leu Ser Lys Glu Ile Arg Pro Val Cys Lys Lys Phe
225 230 235 240
Met Gln Asp Val Ile Gln Asn Ser Tyr Asn Thr Gln Phe Cys Asn Asp
245 250 255
Ala Pro Thr Arg Asn Val
260
<210> 263
<211> 245
<212> PRT
<213> 玉米根萤叶甲属
<400> 263
Met Pro Val Ile Asp Gly Tyr Lys Val Leu Tyr Ile Leu Leu His Ser
1 5 10 15
Leu Tyr Thr Ile Phe Glu Asn Ile Trp Arg Thr Leu Leu Phe Ile Tyr
20 25 30
Gln Asn Cys Ile Arg Val Ile Asn Pro Glu Ser Thr Phe Asp Asp Ala
35 40 45
Asp Gln Leu Lys Lys Arg Leu Ser Arg Leu Thr Lys Lys Pro Gln His
50 55 60
Leu Thr Ile Ile Ile Gly Val Glu Glu Tyr Ser Leu Val Asp Leu Ala
65 70 75 80
Asn Leu Val Tyr Trp Cys Leu Gly Leu Asn Ile Pro Tyr Val Ser Phe
85 90 95
Tyr Asp Tyr Lys Gly Asn Leu Lys Lys His Glu Glu Lys Leu Gln Gln
100 105 110
Ile Val Glu Ser Arg Lys Ser Glu Asn Ile Asn Ile Ile Trp His Thr
115 120 125
His Ala Glu Gln Arg His Lys Asn Gly Phe Leu Gly Pro Lys Ile His
130 135 140
Val Lys Val Leu Thr His Ala Asp Gly Lys Gln Ser Ile Val Asn Val
145 150 155 160
Thr Lys Lys Leu Ala Leu Asn Lys Glu Lys Asp Ile Ser Lys Glu Lys
165 170 175
Ile Ser Glu Leu Leu Leu Arg Gln Tyr Glu Phe Pro Asp Pro Glu Met
180 185 190
Ala Ile Ile Cys Gly Lys Lys Leu Asn Ile Tyr Asn Tyr Pro Pro Trp
195 200 205
Gln Leu Arg Leu Thr Glu Phe Phe Lys Val Asn Lys Val Asn Asn Ile
210 215 220
Thr Phe Pro Val Phe Val Glu Lys Leu Glu Lys Tyr Ser Lys Cys Glu
225 230 235 240
Gln Arg Val Gly Lys
245
<210> 264
<211> 159
<212> PRT
<213> 玉米根萤叶甲属
<400> 264
Met Thr Asn Ser Lys Gly Tyr Arg Arg Gly Thr Arg Asp Leu Phe Ala
1 5 10 15
Arg Lys Phe Lys Lys Arg Gly Val Ile Pro Leu Ser Thr Tyr Leu Arg
20 25 30
Val Tyr Lys Val Gly Asp Ile Val Asp Ile Lys Gly Asn Gly Ala Val
35 40 45
Gln Lys Gly Met Pro His Lys Val Tyr His Gly Lys Thr Gly Arg Val
50 55 60
Phe Asn Val Thr Ala His Ala Leu Gly Val Ile Val Asn Lys Arg Val
65 70 75 80
Arg Gly Arg Ile Ile Pro Lys Arg Ile Asn Leu Arg Ile Glu His Val
85 90 95
Asn His Ser Lys Cys Arg Gln Asp Phe Leu Gln Arg Val Lys Ser Asn
100 105 110
Glu Lys Leu Arg Lys Glu Ala Lys Glu Lys Asn Ile Lys Val Glu Leu
115 120 125
Arg Arg Gln Pro Ala Gln Pro Arg Pro Ala His Ile Val Ser Gly Lys
130 135 140
Val Pro Ala Gln Val Leu Ala Pro Ile Pro Tyr Glu Phe Ile Ala
145 150 155
<210> 265
<211> 178
<212> PRT
<213> 玉米根萤叶甲属
<400> 265
Met Glu Gly Ile Leu Leu Glu Pro Thr Leu Tyr Thr Ile Lys Gly Ile
1 5 10 15
Ala Ile Leu Asp Tyr Asp Gly Asn Arg Val Leu Ala Lys Tyr Tyr Asp
20 25 30
Lys Asp Ile Phe Pro Thr Ala Lys Glu Gln Lys Ala Phe Glu Lys Asn
35 40 45
Leu Phe Asn Lys Thr His Arg Ala Asp Ala Glu Ile Ile Met Leu Asp
50 55 60
Gly Leu Thr Cys Val Tyr Arg Ser Asn Val Asp Leu Phe Phe Tyr Val
65 70 75 80
Met Gly Ser Ser His Glu Asn Glu Leu Ile Leu Met Ser Val Leu Asn
85 90 95
Cys Leu Tyr Asp Ser Val Ser Gln Ile Leu Lys Lys Asn Met Gln Lys
100 105 110
Arg Ala Val Leu Glu Ser Leu Asp Ile Val Met Leu Ala Met Asp Glu
115 120 125
Ile Val Asp Gly Gly Ile Ile Ile Asp Ser Asp Ser Ser Ser Val Val
130 135 140
Ser Arg Ile Ala Leu Arg Thr Asp Asp Ile Pro Leu Gly Glu Gln Thr
145 150 155 160
Val Ala Gln Val Phe Gln Thr Ala Lys Glu Gln Leu Lys Trp Ser Leu
165 170 175
Leu Lys
<210> 266
<211> 149
<212> PRT
<213> 玉米根萤叶甲属
<400> 266
Met Ala Asp Gln Leu Thr Glu Glu Gln Ile Ala Glu Phe Lys Glu Ala
1 5 10 15
Phe Ser Leu Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu
20 25 30
Leu Gly Thr Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu
35 40 45
Leu Gln Asp Met Ile Asn Glu Val Asp Ala Asp Gly Asn Gly Thr Ile
50 55 60
Asp Phe Pro Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys Asp Thr
65 70 75 80
Asp Ser Glu Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp Lys Asp
85 90 95
Gly Asn Gly Phe Ile Ser Ala Ala Glu Leu Arg His Val Met Thr Asn
100 105 110
Leu Gly Glu Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu
115 120 125
Ala Asp Ile Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe Val Thr
130 135 140
Met Met Thr Ser Lys
145
<210> 267
<211> 670
<212> PRT
<213> 玉米根萤叶甲属
<400> 267
Met Met Gln Ala Asn Asn Arg Val Pro Pro Ile Lys Leu Glu Asn Asp
1 5 10 15
Ile Asp Leu Tyr Ala Asp Asp Ile Glu Asp Phe Ala Gln Asp Asp Phe
20 25 30
Gly Gly Glu Asn Val Asp Leu Tyr Asp Asp Val Ile Ser Ala Pro Pro
35 40 45
Gly Asn Asn Asp Asn Pro Gly Asp Ser Asn His His Ala Pro Pro Gly
50 55 60
Ala Gly Glu Asp Gly Gly Gly Asn Phe Val Gly Ser Gly Gly Ala Pro
65 70 75 80
Asn Asn Ile Asn Ser Ser Gly Arg Arg His Gln Leu Tyr Val Gly Asn
85 90 95
Leu Thr Trp Trp Thr Thr Asp Gln Asp Ile Glu Asn Ala Val His Asp
100 105 110
Ile Gly Val Thr Asp Phe His Glu Val Lys Phe Phe Glu His Arg Ala
115 120 125
Asn Gly Gln Ser Lys Gly Phe Cys Val Ile Ser Leu Gly Ser Glu Gly
130 135 140
Ser Met Arg Leu Cys Leu Glu Leu Leu Ser Lys Lys Glu Ile Asn Gly
145 150 155 160
Gln Asn Pro Leu Val Thr Leu Pro Thr Lys Gln Ala Leu Ser Asn Phe
165 170 175
Glu Ser Gln Ser Lys Thr Arg Pro Ser Pro Thr Asn Asn Ser Asn Ser
180 185 190
Arg Pro Pro His Pro Asn Asn Asn Val His Ser Gly Pro Met Gln Asn
195 200 205
Tyr Gly Gly Arg Met Pro Met Asn Pro Ser Met Arg Pro Met Pro Pro
210 215 220
Gly Met Gln Gly Ala Pro Arg Met Gln Gly Pro Pro Gly Phe Asn Gly
225 230 235 240
Pro Pro Asn Met Asn Gln Gln Pro Pro Arg Phe Gln Gly Asn Pro Gln
245 250 255
Trp Asn Gly Pro Arg Pro Asn Gly Pro Gly Pro Asn Met Gly Met Arg
260 265 270
Pro Met Gly Pro Pro His Gly Gln Gln Gly Pro Pro Arg Pro Pro Met
275 280 285
Gln Gly Pro Pro Gln Gln Gly Pro Pro Arg Gly Met Pro Pro Gln Gly
290 295 300
Pro Pro Gln Met Arg Pro Glu Trp Asn Arg Pro Pro Met Gln Gln Gly
305 310 315 320
Tyr Pro Gln Gly Pro Pro His Met Gln Gly Pro Asn Met Gly Pro Arg
325 330 335
Gly Pro Pro Gln Met Gly Pro Pro Gly Ala Pro Gln Gln Gln Gly Pro
340 345 350
Ala Pro His Val Asn Pro Ala Phe Phe Gln Gln Gly Gly Gly Pro Pro
355 360 365
Pro Pro Met Gln His Met Pro Gly Pro Gly Pro Val Met Pro Pro Gln
370 375 380
Gly Pro Pro Gln Gly Pro Pro His Gly Pro Val Gly Pro Pro His Gly
385 390 395 400
Pro Pro Leu Gly Pro Ala Asn Val Pro Pro His Gly Pro Pro His Gly
405 410 415
Tyr Gly Pro Pro Ala Ala Met Pro Gln Pro Pro Tyr Gly Gly Pro Pro
420 425 430
Pro Asp His Arg Ala Glu Ile Pro Gln Leu Thr Glu Gln Glu Phe Glu
435 440 445
Asp Ile Met Ser Arg Asn Arg Thr Val Ser Ser Ser Ala Ile Gly Arg
450 455 460
Ala Val Ser Asp Ala Ala Ala Gly Glu Phe Ala Ser Ala Ile Glu Thr
465 470 475 480
Leu Val Thr Ala Ile Ser Leu Ile Lys Gln Ser Lys Val Ala Asn Asp
485 490 495
Asp Arg Cys Lys Ile Leu Ile Ser Ser Leu Gln Asp Thr Leu Arg Gly
500 505 510
Val Glu Asp Lys Ser Tyr Ser Ser Ser Arg Arg Asp Arg Ser Arg Ser
515 520 525
Arg Asp Arg Ser His Arg Arg Thr Arg Arg Glu Arg Ser Ser Ser Arg
530 535 540
Tyr Arg Asp Arg Ser Arg Glu Arg Glu Arg Glu Arg Asp Arg Asp Arg
545 550 555 560
Asp Arg Glu Arg Asp Arg Tyr Tyr Asp Arg Tyr Ser Glu Arg Glu Arg
565 570 575
Asp Arg Asp Arg Ser Arg Ser Arg Glu Arg Thr Glu Arg Asp Arg Glu
580 585 590
Arg Asp Tyr Arg Asp Arg Glu Pro Glu Glu Thr Asp Lys Glu Lys Ser
595 600 605
Lys Val Ser Arg Val Ser Arg Ser Arg Asn Lys Ser Pro Glu Pro Val
610 615 620
Glu Pro Ser Ser Glu Val Pro Lys Ser Ser Arg Tyr Tyr Glu Asp Arg
625 630 635 640
Tyr Arg Glu Arg Glu Arg Glu Gly Arg Arg Glu Ser Asp Arg Glu Arg
645 650 655
Glu Arg Asp Arg Arg Gly Glu Asp Ser His Arg Ser Arg His
660 665 670
<210> 268
<211> 254
<212> PRT
<213> 玉米根萤叶甲属
<400> 268
Met Ser Ser Ile Gly Thr Gly Tyr Asp Leu Ser Ala Ser Gln Phe Ser
1 5 10 15
Pro Asp Gly Arg Val Phe Gln Val Glu Tyr Ala Met Lys Ala Val Glu
20 25 30
Asn Ser Gly Thr Val Ile Gly Leu Arg Gly Thr Asp Gly Ile Val Leu
35 40 45
Ala Ala Glu Lys Leu Ile Met Ser Lys Leu His Glu Pro Ser Thr Asn
50 55 60
Lys Arg Ile Phe Asn Ile Asp Lys His Ile Gly Met Ala Phe Ser Gly
65 70 75 80
Leu Ile Ala Asp Ala Arg Gln Ile Val Glu Ile Ala Arg Lys Glu Ala
85 90 95
Ser Asn Tyr Arg His Gln Tyr Gly Ser Asn Ile Pro Leu Lys Tyr Leu
100 105 110
Asn Asp Arg Val Ser Met Tyr Met His Ala Tyr Thr Leu Tyr Ser Ala
115 120 125
Val Arg Pro Phe Gly Cys Ser Val Ile Leu Ala Ser Tyr Glu Asp Ser
130 135 140
Asp Pro Ser Met Tyr Leu Ile Asp Pro Ser Gly Val Ser Tyr Gly Tyr
145 150 155 160
Phe Gly Cys Ala Thr Gly Lys Ala Lys Gln Ser Ala Lys Thr Glu Ile
165 170 175
Glu Lys Leu Lys Met Gly Asn Leu Thr Cys Lys Glu Leu Val Lys Glu
180 185 190
Ala Ala Lys Ile Ile Tyr Leu Val His Asp Glu Leu Lys Asp Lys Asn
195 200 205
Phe Glu Leu Glu Leu Ser Trp Val Cys Lys Asp Thr Asn Gly Leu His
210 215 220
Thr Lys Val Pro Glu Ser Val Phe Ala Asp Ala Glu Lys Ala Ala Lys
225 230 235 240
Gln Ala Met Glu Ala Asp Ser Glu Ser Asp Thr Glu Asp Met
245 250
<210> 269
<211> 246
<212> PRT
<213> 玉米根萤叶甲属
<400> 269
Met Ala Ser Lys Asp Arg Leu Met Ile Phe Pro Ser Arg Gly Ala Gln
1 5 10 15
Met Met Met Lys Ser Arg Leu Lys Gly Ala Gln Lys Gly His Ser Leu
20 25 30
Leu Lys Lys Lys Ala Asp Ala Leu Gln Met Arg Phe Arg Met Ile Leu
35 40 45
Asn Lys Ile Ile Glu Thr Lys Thr Leu Met Gly Glu Val Met Lys Glu
50 55 60
Ala Ala Phe Ser Leu Ala Glu Ala Lys Phe Ala Thr Gly Asp Phe Asn
65 70 75 80
Gln Val Val Leu Gln Asn Val Thr Lys Ala Gln Ile Lys Ile Arg Thr
85 90 95
Lys Lys Asp Asn Val Ala Gly Val Thr Leu Pro Val Phe Glu Cys Tyr
100 105 110
Gln Asp Gly Thr Asp Thr Tyr Glu Leu Ala Gly Leu Ala Arg Gly Gly
115 120 125
Gln Gln Leu Thr Lys Leu Lys Lys Asn Tyr Gln Ser Ala Val Lys Leu
130 135 140
Leu Val Glu Leu Ala Ser Leu Gln Thr Ser Phe Val Thr Leu Asp Asp
145 150 155 160
Val Ile Lys Ile Thr Asn Arg Arg Val Asn Ala Ile Glu His Val Ile
165 170 175
Ile Pro Arg Ile Glu Arg Thr Leu Ala Tyr Ile Ile Ser Glu Leu Asp
180 185 190
Glu Leu Glu Arg Glu Glu Phe Tyr Arg Leu Lys Lys Ile Gln Asp Lys
195 200 205
Lys Lys Ile Ser Arg Ala Lys Ala Glu Lys Gln Lys Gln Ala Leu Leu
210 215 220
Gln Ala Gly Leu Leu Lys Glu Ser Gln Ala Asn Met Leu Leu Asp Glu
225 230 235 240
Gly Asp Glu Asp Leu Leu
245
<210> 270
<211> 1605
<212> PRT
<213> 玉米根萤叶甲属
<400> 270
Met Glu Ala Ala Pro Lys Leu Pro Met Leu Ser Phe Glu Leu Asn Thr
1 5 10 15
Cys Thr Glu Asn Val His Phe Gly Pro Gln Leu Lys Gln Tyr Ile Ala
20 25 30
Ala Phe Tyr Gly Glu Asp Pro Glu Ser Tyr Ile Thr Glu Ile Ser Asn
35 40 45
Leu Glu Ser Leu Arg Ser Ala Ala Val Arg Pro Ser Thr Asp Val Asn
50 55 60
Gly Val Gln Leu Leu Lys Lys Tyr Phe Cys Gln Leu Arg Phe Leu Lys
65 70 75 80
Ser Arg Phe Pro Met Glu Glu Asn Gln Asp Ala Ala Val Leu Phe Ser
85 90 95
Trp Lys Asn Asn Glu Leu Asp Ile Thr Ser Thr Ser Ser Asp Ile Arg
100 105 110
Tyr Glu Leu Met Val Ile Met Tyr Asn Ile Gly Ala Leu His Thr Phe
115 120 125
Leu Gly Ala Asn Asp Ser Arg Asn Asn Pro Asp Gly Met Lys Met Ala
130 135 140
Cys Thr His Phe Gln Cys Ala Ala Trp Ala Phe Gln Asn Val Lys Glu
145 150 155 160
Lys Tyr His Gln Phe Ile Ser Asn Ile Ser Leu Val Glu Leu Val His
165 170 175
Phe Phe Gln Gln Val Cys Leu Ala Gln Ala Gln Glu Cys Ile Leu Glu
180 185 190
Lys Ser Met Phe Asp Asn Arg Lys Pro Thr Ile Ile Ala Lys Val Ala
195 200 205
Ile Gln Val Tyr Ser Tyr Tyr Arg Gln Ser Leu Arg Val Leu Glu Ser
210 215 220
Val Asn Glu Ala Tyr Phe Arg Asp Lys Thr Tyr Lys Glu Trp Met Lys
225 230 235 240
Tyr Leu Gln Phe Lys Leu Thr Tyr Tyr Lys Cys Ile Ser Phe Leu Phe
245 250 255
Gln Gly Gln Gln Ala Glu Glu Gln Gln Lys Met Gly Glu Arg Val Ala
260 265 270
Phe Tyr Gln Ala Ala Cys Glu Gln Leu Asp Glu Ala Lys Lys Ile Ala
275 280 285
Ala Thr Leu Lys Asn Gln His His Gln Gln Glu Ile Asn Glu Gly Leu
290 295 300
Ala Phe Thr Thr Asp Val Val Glu Gly Lys Arg Lys Ala Ala Lys Asn
305 310 315 320
Glu Asn Glu Phe Ile Tyr His Glu Ser Val Pro Asp Lys Asp Gln Leu
325 330 335
Pro Glu Val Lys Gly Ala Ser Leu Val Lys Gly Ile Pro Phe Ser Ile
340 345 350
Asn Asp Ile Glu Val Ser Gly Pro Asp Ile Phe Ser Arg Leu Val Pro
355 360 365
Met Glu Ala His Glu Ala Ala Ser Leu Tyr Ser Glu Lys Lys Ala Gln
370 375 380
Arg Leu Arg Gln Ile Gly Glu Leu Ile Glu Asn Lys Asp Gln Thr Leu
385 390 395 400
Ala Glu Phe Met Ser Ser Met Gln Leu Asp Leu Leu Thr Lys Met His
405 410 415
Gln Ala Thr Gly Ile Pro Gln Glu Leu Ile Asp Arg Ala Ala Ala Leu
420 425 430
Ser Ala Lys Pro Asn Ala Ile Gln Asp Leu Ile Ser Ala Met Gly Lys
435 440 445
Leu Ser Asn Ile Tyr Gln Asp Val Glu Ala Ser Leu Asn Glu Ile Asp
450 455 460
Ser Leu Leu Lys Ala Glu Glu Gln Ser Glu Gln Lys Tyr Gln Glu Thr
465 470 475 480
Ile Gly Lys Arg Pro Pro Ser Ile Leu Ala Thr Asp Leu Thr Arg Glu
485 490 495
Ala Ala Lys Tyr Arg Glu Ala His Thr Lys Ala Asn Asp Ser Asn Gln
500 505 510
Thr Leu His Arg Ala Met Met Ala His Val Ala Asn Leu Lys Ile Leu
515 520 525
Gln Gln Pro Leu Lys Gln Leu Gln His Gln Leu Pro Phe Val Glu Phe
530 535 540
Pro Asn Pro Asn Ile Asp Glu Lys Ser Leu Lys Asp Leu Glu Ala Leu
545 550 555 560
Val Ala Lys Val Asp Glu Met Arg Thr Gln Arg Ala Met Leu Trp Ala
565 570 575
Gln Leu Arg Glu Ser Ile His Gln Asp Asp Ile Thr Ser Ser Leu Val
580 585 590
Thr Lys Gln Pro Asn Gln Ser Leu Glu Gln Leu Phe Gln Gln Glu Leu
595 600 605
Gln Lys His Gln Asn Leu Ile Ser Leu Ile Glu Gln Asn Thr Ser Ala
610 615 620
Gln Glu Asn Ile Lys Ser Ala Leu Val Asp Ser Tyr Ala Tyr Ala Val
625 630 635 640
Asn Ser Arg Lys Tyr Ile Gln Asp Ile Leu Gln Lys Arg Thr Thr Thr
645 650 655
Ile Thr Ser Leu Ile Ala Ser Phe Asp Ser Tyr Glu Asp Leu Leu Ala
660 665 670
Lys Ala Asn Lys Gly Ile Glu Phe Tyr Ser Lys Leu Glu Thr Asn Val
675 680 685
Ser Lys Leu Leu Gln Arg Ile Arg Ser Thr Cys Lys Val Gln Gln Glu
690 695 700
Glu Arg Asp Gln Met Met Ser Thr Ala Gln Val Pro Gln Trp Glu Ser
705 710 715 720
His Thr Ser Leu Ala Ala Pro Lys Leu Lys Asp Tyr Leu Asp Ser Arg
725 730 735
Lys Lys Ser Ala Ala Tyr Ser Glu Pro Ser Val Gln Pro Gln Gln Pro
740 745 750
Thr Leu Ser Tyr Ser Ala Ala Met Asp Leu Pro Pro Gly Ile Arg Pro
755 760 765
Thr Pro Val Gly Ser Glu Ile Thr Asp Val Pro Lys Asn Ile Gln Gly
770 775 780
Glu Pro Gln Gly Tyr Ile Pro Tyr Asn Tyr Gln Gln Pro Ser Val Pro
785 790 795 800
Ala Ser Gln Asn Ile Asp Glu Glu Thr Ile Lys Lys Met Asn Ala Leu
805 810 815
Met Pro Gly Ala Lys Thr Ser Val Pro Ser Gln Tyr Gly Tyr Ser Asn
820 825 830
Tyr Ile Pro Pro Thr Tyr Pro Gln Ser Ala Tyr Gln Pro Gly Asn Gln
835 840 845
Ser Tyr Gly Lys Glu Thr Pro Asp Ile Asn Ser Pro Tyr Asp Pro Thr
850 855 860
Lys Ala Phe Thr Ala Thr Thr Asn Ala Tyr Arg Ser Val Gln Ser Ser
865 870 875 880
Ser Thr Gln Gly Tyr Val Pro Tyr Ala Glu Ser Asn Val Ser Asn Val
885 890 895
Asp Arg Val Gly Tyr Pro Ser Arg Tyr Gln Tyr Gln Gln Val Pro Glu
900 905 910
Ile Ala Thr Thr Pro Ala Asp Pro Asn Ile Asn Ala Tyr Tyr Pro His
915 920 925
Gly Tyr Ser Pro Ser Gln Asn Leu Pro Asn Ala Asn Thr Gln His Ile
930 935 940
Thr Gly Gln Leu Lys Tyr His Ser Val Glu Tyr Ala Ser Ser Val Pro
945 950 955 960
Asn Asn Ile Asn Tyr Asn Ser Ser Thr Tyr Ser Ser Pro Leu Ser Asn
965 970 975
Met Ser Ser Thr Asn Ser Ser Asn Pro Ser Asn Leu Asn Asn Ser Tyr
980 985 990
Glu Tyr Tyr Tyr Asp Pro Asn Thr Ser Ser Gly Ala Val Pro Asn Ala
995 1000 1005
Ser Lys Pro Gln Gln Ser Ser Ala Ser Ser Ala Asn Pro Ser Thr
1010 1015 1020
Ala Met Asn Asn Tyr Asn Tyr Tyr Tyr Asn Thr Ser Thr Ser Gly
1025 1030 1035
Ser Val Ala Ala Asp Thr Ser Lys Ile Gln Gln Gln Gln Gln Tyr
1040 1045 1050
Pro Gly Thr Gln Met Ser Gln Ala Gln Tyr Tyr Pro Ala Asn Ala
1055 1060 1065
Ser Tyr Tyr Ser Thr Ser Thr Tyr Asn Thr Asn Val Gln Gly Gly
1070 1075 1080
Thr Asn Pro Ser Tyr Ala Thr Gly Gln Thr Tyr Asn Gln Val Thr
1085 1090 1095
Pro Val Thr Ser Gln Asn Val Ser Gln Asn Tyr Asn Phe Asn Gln
1100 1105 1110
Val Gly Ser Gly Ala Gly His Gln His Gln Tyr Tyr Ser Ser Ala
1115 1120 1125
Asn Ala Ala Val Pro Ser Gln Gln Ala Val Asn Asn Ser Ser Leu
1130 1135 1140
Pro Asn Tyr Gly Tyr Asp Gln Tyr Tyr Gly Asn Asn Tyr Asn Ser
1145 1150 1155
Ser Gln Pro Ser Thr Tyr Ser Ala Asn Gln Ala Pro Pro Ala Ala
1160 1165 1170
Gln Ala Ala Pro Ser Asn Ile Pro Ala Ala Thr Lys Ser Ser Ser
1175 1180 1185
Asn Val Asp Leu Leu Ser Gly Leu Asp Phe Ser Ile Ser Gln Ala
1190 1195 1200
Pro Leu Val Pro Gln Gln Asn Ile Thr Ile Lys Pro Gln Glu Lys
1205 1210 1215
Glu Thr Lys Pro Pro Ala Val Ser Ser Glu Thr Lys Asn Gln Asp
1220 1225 1230
Pro Thr Pro Val Thr Thr Pro Lys Gln Pro Thr Gly Pro Glu Val
1235 1240 1245
Lys Arg Leu Tyr Val Lys Ile Leu Pro Ser Lys Pro Leu Asn Asn
1250 1255 1260
Asp Asp Val Lys Lys Leu Phe Gly Gln Glu Leu Asp Arg Tyr Glu
1265 1270 1275
Lys Phe Val Glu Thr Leu Thr His Lys Thr Leu Ser Gly Pro Thr
1280 1285 1290
Thr Leu Asp Ile Lys Trp Lys Glu Ile Gln Asp Gln Gln Asp Cys
1295 1300 1305
Glu Pro Gln Lys Lys Ile Ile Ser Val Ala Arg Cys Tyr Pro Met
1310 1315 1320
Lys Asn Arg Phe Pro Asp Ile Leu Pro Tyr Asp Phe Ser Arg Val
1325 1330 1335
Glu Leu Cys Asp Ser Lys Asp Asp Tyr Ile Asn Ala Ser Tyr Ile
1340 1345 1350
Lys Asp Ile Ser Pro Tyr Ala Pro Ser Phe Ile Val Thr Gln Val
1355 1360 1365
Pro Leu Ser Ser Thr Val Gly Asp Met Trp Thr Met Ile Arg Glu
1370 1375 1380
Gln Gln Val Glu Leu Ile Leu Cys Leu Val Asn Asp Asn Glu Ile
1385 1390 1395
Gly Glu Asp Ile Tyr Trp Pro Lys Glu Lys Gly Ser Ser Leu Asn
1400 1405 1410
Ile Leu Asn Met Val Ile Thr Leu Gln Asn Val Ile Val Lys Ser
1415 1420 1425
His Trp Thr Glu Arg Leu Ile Ala Ile Asn Leu Pro Glu Lys Arg
1430 1435 1440
Glu Ser Arg Val Ile Met His Leu Gln Phe Thr Ser Trp Pro Gly
1445 1450 1455
Ser Leu Phe Pro Thr Asn Pro Glu Pro Phe Val Ser Tyr Thr Leu
1460 1465 1470
Glu Ser Ile Asn Leu Tyr Gln Gln Gln Lys Thr Asn Thr His Pro
1475 1480 1485
Val Val Val His Cys Ser Ser Gly Ile Gly Arg Ser Gly Leu Leu
1490 1495 1500
Cys Leu Leu Thr Ala Ala Met Phe Asp Ala Ala Asn Asn Ala Asn
1505 1510 1515
Ser Ile Pro Asp Leu Thr Ala Leu Ser Ile Lys Leu Ser Asn Cys
1520 1525 1530
Arg Lys Asn Ile Leu Arg Asp Arg Glu His Leu Lys Phe Gly Tyr
1535 1540 1545
Glu Ser Phe Leu Ala Tyr Ile Arg His Ile Val Cys Glu Asp Lys
1550 1555 1560
Ala Arg Lys Lys Leu Asn Glu Ile Gln Pro Lys Val Lys Glu Glu
1565 1570 1575
Pro Leu Glu Pro Pro Val Ile Val Pro Glu Pro Asn Ile Asp Pro
1580 1585 1590
Leu Ser Thr Leu Asp Pro Phe Trp Ala Ser Lys Arg
1595 1600 1605
<210> 271
<211> 513
<212> DNA
<213> 巴氏根萤叶甲
<400> 271
atgaaacctt tcttggcccc tgagagaaag aagaaactta ggttactgtt gagaaagaaa 60
gccgctgaag aattaaagaa agaacaagaa cgcaaagcag ccgaaaggag gcgtattatt 120
gaagaaaggt gcggtaaacc caaacttgtc gacgacgcaa atgaaggctc attaaaacaa 180
gtatgtgagg gatatcacag acgtattgta gacctagaaa ataagaaatt tgacctcgaa 240
aaggaagtgg aattcagaga ttttcagatc tccgaattga acagccaagt aaacgacctt 300
agaggcaaat tcgtcaaacc aaccttgaag aaggtatcca aatacgaaaa caaattcgcc 360
aaacttcaaa agaaggcagc tgaatttaac ttccgtaacc aactcaaagt tgtcaagaag 420
aaagaattca ccttagaaga agaagacaaa gaaaagaaac cagactggtc aaagaaggga 480
gacgaaaaga aggtacaaga ggctgaagca tga 513
<210> 272
<211> 279
<212> DNA
<213> 巴氏根萤叶甲
<400> 272
atgaagtttt taaaatcgac agtgtgctac attgccatct tggcaattct ctttaccctc 60
tgtgccgatg aggttgaagg aaggagaaaa attttgatgg ggcgaaaaag cattaccagg 120
acatatcttc gtggaaatgc tgttcctgcg tatgtgataa taatccttgt aggaattggt 180
caaatcatcc ttggagggat attgtacgtg gcattgaaga agaagatcat tgatgcacct 240
gtaacgggat catatgcagt ggctagacaa gaaccataa 279
<210> 273
<211> 651
<212> DNA
<213> 巴氏根萤叶甲
<400> 273
atggcggcaa acagaactgg acctgctcag agaccaaatg gcgctaccca aggaaagata 60
tgtcagttca aactggtcct actaggcgaa agtgccgtcg gtaagtcgag tttggtactg 120
aggttcgtca aaggacagtt ccacgaatac caggagagta ccataggagc agctttcctt 180
acacaaacca tatgcctcga cgatacaact gttaaatttg aaatttggga cacagcgggt 240
caagaaaggt accacagttt agctcctatg tactataggg gcgcacaggc agctatagtc 300
gtctacgaca taaccaatca agacacattc ggcagggcga aaacgtgggt gaaggaactt 360
caaaggcagg ccagtccgac gatcgtgata gctttggccg gcaacaagca ggatttggcc 420
aacaaacgta tggtagaata cgaagaggcg caaacgtatg ctgacgaaaa cggcttactt 480
tttatggaaa cttccgcaaa gacggcaatg aacgtcaacg atatattttt agcaatagct 540
aagaaactgc ccaagaatga acaaaccaca ggtcaaggcg gcagtgccca aggcaggcgg 600
ctagcggaag gcgattcggg cgccaaggca cccggaaatt gttgcaagtg a 651
<210> 274
<211> 513
<212> RNA
<213> 巴氏根萤叶甲
<400> 274
augaaaccuu ucuuggcccc ugagagaaag aagaaacuua gguuacuguu gagaaagaaa 60
gccgcugaag aauuaaagaa agaacaagaa cgcaaagcag ccgaaaggag gcguauuauu 120
gaagaaaggu gcgguaaacc caaacuuguc gacgacgcaa augaaggcuc auuaaaacaa 180
guaugugagg gauaucacag acguauugua gaccuagaaa auaagaaauu ugaccucgaa 240
aaggaagugg aauucagaga uuuucagauc uccgaauuga acagccaagu aaacgaccuu 300
agaggcaaau ucgucaaacc aaccuugaag aagguaucca aauacgaaaa caaauucgcc 360
aaacuucaaa agaaggcagc ugaauuuaac uuccguaacc aacucaaagu ugucaagaag 420
aaagaauuca ccuuagaaga agaagacaaa gaaaagaaac cagacugguc aaagaaggga 480
gacgaaaaga agguacaaga ggcugaagca uga 513
<210> 275
<211> 279
<212> RNA
<213> 巴氏根萤叶甲
<400> 275
augaaguuuu uaaaaucgac agugugcuac auugccaucu uggcaauucu cuuuacccuc 60
ugugccgaug agguugaagg aaggagaaaa auuuugaugg ggcgaaaaag cauuaccagg 120
acauaucuuc guggaaaugc uguuccugcg uaugugauaa uaauccuugu aggaauuggu 180
caaaucaucc uuggagggau auuguacgug gcauugaaga agaagaucau ugaugcaccu 240
guaacgggau cauaugcagu ggcuagacaa gaaccauaa 279
<210> 276
<211> 651
<212> RNA
<213> 巴氏根萤叶甲
<400> 276
auggcggcaa acagaacugg accugcucag agaccaaaug gcgcuaccca aggaaagaua 60
ugucaguuca aacugguccu acuaggcgaa agugccgucg guaagucgag uuugguacug 120
agguucguca aaggacaguu ccacgaauac caggagagua ccauaggagc agcuuuccuu 180
acacaaacca uaugccucga cgauacaacu guuaaauuug aaauuuggga cacagcgggu 240
caagaaaggu accacaguuu agcuccuaug uacuauaggg gcgcacaggc agcuauaguc 300
gucuacgaca uaaccaauca agacacauuc ggcagggcga aaacgugggu gaaggaacuu 360
caaaggcagg ccaguccgac gaucgugaua gcuuuggccg gcaacaagca ggauuuggcc 420
aacaaacgua ugguagaaua cgaagaggcg caaacguaug cugacgaaaa cggcuuacuu 480
uuuauggaaa cuuccgcaaa gacggcaaug aacgucaacg auauauuuuu agcaauagcu 540
aagaaacugc ccaagaauga acaaaccaca ggucaaggcg gcagugccca aggcaggcgg 600
cuagcggaag gcgauucggg cgccaaggca cccggaaauu guugcaagug a 651
<210> 277
<211> 744
<212> DNA
<213> 黄瓜十一星叶甲
<400> 277
atggcggacg atgaggaaaa gaagaggaaa caggccgaaa ttgaacgcaa aagggccgag 60
gtcagggctc gtatggaaga ggcctcaaaa gccaagaagg ccaagaaagg tttggcggac 120
gatgagagaa agaaactgga ggaggaaaag aagaggaaac aggccgaaat tgaacgcaaa 180
agggccgagg tcagggctcg tatggaagag gcctcaaaag ccaagaaggc caagaaaggt 240
ttcatgaccc ctgagagaaa gaagaaactt aggttactgt tgagaaagaa agccgctgaa 300
gaattaaaga aagaacaaga acgcaaagca gctgaaagga ggcgtatcat tgaagaaagg 360
tgcggtaaac ccaaacttgt cgatgatgca aatgaagcct cattaaaaca agtatgtgag 420
ggatatcaca gacgtattgt agacctagaa aataagaaat ttgacctcga aaaggaagtg 480
gaattcagag attttcagat ctccgaattg aacagccaag taaacgacct tagaggcaaa 540
ttcgtcaaac caaccttgaa gaaggtatcc aaatacgaaa acaaattcgc caaactccaa 600
aagaaggcag ctgaatttaa cttccgtaac caactcaaag ttgtcaagaa gaaagaattc 660
accttagaag aagaagacaa agaaaagaaa ccagactggt caaagaaggg agacgaaaag 720
aaggtacaag aggctgaagc atga 744
<210> 278
<211> 279
<212> DNA
<213> 黄瓜十一星叶甲
<400> 278
atgaagtttt taaaatcgac agtgtgctac attgccatct tggcaattct ctttaccctc 60
tgtgccgatg aggttgaagg aaggagaaaa attttgatgg ggcgaaaaag cattaccagg 120
acatatcttc gtggaaatgc tgttcctgcg tatgtgataa taatccttgt aggaattggt 180
caaatcatcc ttggagggat attgtacgtg gcattgaaga agaagatcat tgatgcacct 240
gtaacgggat catatgcagt ggctagacaa gaaccataa 279
<210> 279
<211> 651
<212> DNA
<213> 黄瓜十一星叶甲
<400> 279
atggcggcaa acagaactgg acctgctcag agaccaaatg gcgctaccca aggaaagata 60
tgccagttca aacttgtcct actaggcgaa agtgccgtcg gtaagtcgag tttggtactg 120
aggtttgtca aaggacagtt ccacgaatac caggagagta ccataggagc agctttcctt 180
acacaaacca tatgcctcga cgatacaacc gttaaatttg aaatttggga cacagcgggt 240
caagaaaggt accacagttt agctcctatg tactataggg gcgcacaggc agctatagtc 300
gtctacgaca taaccaatca agacacattc ggcagggcga aaacgtgggt taaggaactt 360
caaaggcagg cgagtccgac gatcgtgata gctttggccg gcaacaagca ggatttggcc 420
aataaacgta tggtagaata cgaagaggcg caaacgtatg ctgacgaaaa tggcttactt 480
tttatggaaa cttccgcaaa gacggcaatg aacgtcaacg atatattttt agcaatagct 540
aagaaactgc ccaagaatga acaaacaaca ggtcaaggag gcagtgccca aggtaggcgg 600
ctagcagaag gcgattcggg ggccaaggca cctggaaact gttgcaagtg a 651
<210> 280
<211> 744
<212> RNA
<213> 黄瓜十一星叶甲
<400> 280
auggcggacg augaggaaaa gaagaggaaa caggccgaaa uugaacgcaa aagggccgag 60
gucagggcuc guauggaaga ggccucaaaa gccaagaagg ccaagaaagg uuuggcggac 120
gaugagagaa agaaacugga ggaggaaaag aagaggaaac aggccgaaau ugaacgcaaa 180
agggccgagg ucagggcucg uauggaagag gccucaaaag ccaagaaggc caagaaaggu 240
uucaugaccc cugagagaaa gaagaaacuu agguuacugu ugagaaagaa agccgcugaa 300
gaauuaaaga aagaacaaga acgcaaagca gcugaaagga ggcguaucau ugaagaaagg 360
ugcgguaaac ccaaacuugu cgaugaugca aaugaagccu cauuaaaaca aguaugugag 420
ggauaucaca gacguauugu agaccuagaa aauaagaaau uugaccucga aaaggaagug 480
gaauucagag auuuucagau cuccgaauug aacagccaag uaaacgaccu uagaggcaaa 540
uucgucaaac caaccuugaa gaagguaucc aaauacgaaa acaaauucgc caaacuccaa 600
aagaaggcag cugaauuuaa cuuccguaac caacucaaag uugucaagaa gaaagaauuc 660
accuuagaag aagaagacaa agaaaagaaa ccagacuggu caaagaaggg agacgaaaag 720
aagguacaag aggcugaagc auga 744
<210> 281
<211> 279
<212> RNA
<213> 黄瓜十一星叶甲
<400> 281
augaaguuuu uaaaaucgac agugugcuac auugccaucu uggcaauucu cuuuacccuc 60
ugugccgaug agguugaagg aaggagaaaa auuuugaugg ggcgaaaaag cauuaccagg 120
acauaucuuc guggaaaugc uguuccugcg uaugugauaa uaauccuugu aggaauuggu 180
caaaucaucc uuggagggau auuguacgug gcauugaaga agaagaucau ugaugcaccu 240
guaacgggau cauaugcagu ggcuagacaa gaaccauaa 279
<210> 282
<211> 651
<212> RNA
<213> 黄瓜十一星叶甲
<400> 282
auggcggcaa acagaacugg accugcucag agaccaaaug gcgcuaccca aggaaagaua 60
ugccaguuca aacuuguccu acuaggcgaa agugccgucg guaagucgag uuugguacug 120
agguuuguca aaggacaguu ccacgaauac caggagagua ccauaggagc agcuuuccuu 180
acacaaacca uaugccucga cgauacaacc guuaaauuug aaauuuggga cacagcgggu 240
caagaaaggu accacaguuu agcuccuaug uacuauaggg gcgcacaggc agcuauaguc 300
gucuacgaca uaaccaauca agacacauuc ggcagggcga aaacgugggu uaaggaacuu 360
caaaggcagg cgaguccgac gaucgugaua gcuuuggccg gcaacaagca ggauuuggcc 420
aauaaacgua ugguagaaua cgaagaggcg caaacguaug cugacgaaaa uggcuuacuu 480
uuuauggaaa cuuccgcaaa gacggcaaug aacgucaacg auauauuuuu agcaauagcu 540
aagaaacugc ccaagaauga acaaacaaca ggucaaggag gcagugccca agguaggcgg 600
cuagcagaag gcgauucggg ggccaaggca ccuggaaacu guugcaagug a 651
<210> 283
<211> 478
<212> DNA
<213> 玉米根萤叶甲属
<400> 283
ggtttcatga cccctgagag aaagaagaaa cttaggttac tgttgagaaa gaaagccgcc 60
gaagaattaa agaaagaaca agaacgcaaa gcagccgaaa ggaggcgtat cattgaagaa 120
aggtgcggta aacccaaact tgtcgatgac gcaaatgaag gcccattaaa acaagtatgt 180
gagggatatc acagacgtat tgtagaccta gaaaataaga aatttgacct cgaaaaagaa 240
gtggaattca gagattttca gatctccgaa ttgaacagcc aagtaaacga ccttagaggc 300
aaattcgtca aaccaacctt gaagaaggta tccaaatacg aaaacaaatt cgccaaactt 360
caaaagaagg cagctgaatt taacttccgt aaccaactca aagttgtcaa gaagaaagaa 420
ttcaccttag aagaagaaga caaagaaaag aaaccagact ggtcaaagaa gggagacg 478
<210> 284
<211> 413
<212> DNA
<213> 玉米根萤叶甲属
<400> 284
atggcggacg atgagagaaa gaaactggag gaggaaaaga agaggaaaca ggccgaaatt 60
gaacgcaaaa gggccgaggt cagggctcgt atggaagagg cctcaaaagc caagaaggcc 120
aagaaaggtt tcatgacccc tgagagaaag aagaaactta ggttactgtt gagaaagaaa 180
gccgccgaag aattaaagaa agaacaagaa cgcaaagcag ccgaaaggag gcgtatcatt 240
gaagaaaggt gcggtaaacc caaacttgtc gatgacgcaa atgaaggccc attaaaacaa 300
gtatgtgagg gatatcacag acgtattgta gacctagaaa ataagaaatt tgacctcgaa 360
aaagaagtgg aattcagaga ttttcagatc tccgaattga acagccaagt aaa 413
<210> 285
<211> 100
<212> DNA
<213> 玉米根萤叶甲属
<400> 285
agatctccga attgaacagc caagtaaacg accttagagg caaattcgtc aaaccaacct 60
tgaagaaggt atccaaatac gaaaacaaat tcgccaaact 100
<210> 286
<211> 100
<212> DNA
<213> 玉米根萤叶甲属
<400> 286
tcaaaagaag gcagctgaat ttaacttccg taaccaactc aaagttgtca agaagaaaga 60
attcacctta gaagaagaag acaaagaaaa gaaaccagac 100
<210> 287
<211> 133
<212> DNA
<213> 玉米根萤叶甲属
<400> 287
gaccttagag gcaaattcgt caaaccaacc ttgaagaagg tatccaaata cgaaaacaaa 60
ttcgccaaac ttcaaaagaa ggcagctgaa tttaacttcc gtaaccaact caaagttgtc 120
aagaagaaag aat 133
<210> 288
<211> 500
<212> DNA
<213> 玉米根萤叶甲属
<400> 288
tactaggcga aagtgccgtc ggtaagtcga gtttggtact gaggttcgtc aaaggacagt 60
tccacgaata ccaggagagt accataggag cagctttcct tacacaaacc atatgcctcg 120
acgatacaac tgttaaattt gaaatttggg acacagcggg tcaagaaagg taccacagtt 180
tagctcctat gtactatagg ggcgcacagg cagctatagt cgtctacgac ataaccaatc 240
aagacacatt cggcagggcg aaaacgtggg tgaaggaact tcaaaggcag gccagtccga 300
cgatcgtgat agctttggcc ggcaacaagc aagatttggc caacaaacgt atggtagaat 360
acgaagaggc gcagacgtat gctgacgaaa acggcttact ttttatggaa acttccgcaa 420
agacggcaat gaacgtcaac gatatatttt tagcaatagc taagaaactg cccaagaatg 480
aacaaaccac aggtcaaggc 500
<210> 289
<211> 648
<212> DNA
<213> 玉米根萤叶甲属
<400> 289
atggcggcaa acagaactgg acctgctcag agaccaaatg gcgctaccca aggaaagata 60
tgtcagttca aactggtcct actaggcgaa agtgccgtcg gtaagtcgag tttggtactg 120
aggttcgtca aaggacagtt ccacgaatac caggagagta ccataggagc agctttcctt 180
acacaaacca tatgcctcga cgatacaact gttaaatttg aaatttggga cacagcgggt 240
caagaaaggt accacagttt agctcctatg tactataggg gcgcacaggc agctatagtc 300
gtctacgaca taaccaatca agacacattc ggcagggcga aaacgtgggt gaaggaactt 360
caaaggcagg ccagtccgac gatcgtgata gctttggccg gcaacaagca agatttggcc 420
aacaaacgta tggtagaata cgaagaggcg cagacgtatg ctgacgaaaa cggcttactt 480
tttatggaaa cttccgcaaa gacggcaatg aacgtcaacg atatattttt agcaatagct 540
aagaaactgc ccaagaatga acaaaccaca ggtcaaggcg gcagtgccca aggcaggcgg 600
ctagcggagg gcgattcggg cgccaaggca cccggaaatt gttgcaag 648
<210> 290
<211> 188
<212> DNA
<213> 玉米根萤叶甲属
<400> 290
atggcggcaa acagaactgg acctgctcag agaccaaatg gcgctaccca aggaaagata 60
tgtcagttca aactggtcct actaggcgaa agtgccgtcg gtaagtcgag tttggtactg 120
aggttcgtca aaggacagtt ccacgaatac caggagagta ccataggagc agctttcctt 180
acacaaac 188
<210> 291
<211> 205
<212> DNA
<213> 玉米根萤叶甲属
<400> 291
ggacagttcc acgaatacca ggagagtacc ataggagcag ctttccttac acaaaccata 60
tgcctcgacg atacaactgt taaatttgaa atttgggaca cagcgggtca agaaaggtac 120
cacagtttag ctcctatgta ctataggggc gcacaggcag ctatagtcgt ctacgacata 180
accaatcaag acacattcgg caggg 205
<210> 292
<211> 224
<212> DNA
<213> 玉米根萤叶甲属
<400> 292
gcacaggcag ctatagtcgt ctacgacata accaatcaag acacattcgg cagggcgaaa 60
acgtgggtga aggaacttca aaggcaggcc agtccgacga tcgtgatagc tttggccggc 120
aacaagcaag atttggccaa caaacgtatg gtagaatacg aagaggcgca gacgtatgct 180
gacgaaaacg gcttactttt tatggaaact tccgcaaaga cggc 224
<210> 293
<211> 196
<212> DNA
<213> 玉米根萤叶甲属
<400> 293
gacgtatgct gacgaaaacg gcttactttt tatggaaact tccgcaaaga cggcaatgaa 60
cgtcaacgat atatttttag caatagctaa gaaactgccc aagaatgaac aaaccacagg 120
tcaaggcggc agtgcccaag gcaggcggct agcggagggc gattcgggcg ccaaggcacc 180
cggaaattgt tgcaag 196
<210> 294
<211> 189
<212> DNA
<213> 玉米根萤叶甲属
<400> 294
cgatgaggtt gaaggaagga gaaaaatttt gatggggcga aaaagcatta ccaggacata 60
tcttcgtgga aatgctgttc ctgcgtatgt gataataatc cttgtaggaa ttggtcaaat 120
catcctggga gggatattgt acgttgcatt gaggaagaag atcattgctg cacctgtaac 180
ggcatcata 189
<210> 295
<211> 330
<212> DNA
<213> 玉米根萤叶甲属
<400> 295
gtgtgtattt cagctggaat ttgtaatgaa aaaaacgtag aaatatatac tacaatgaag 60
tttttaagat cgacagtgtg ctacattgcc atcttggcaa ttctctttac cctctgtgcc 120
gatgaggttg aaggaaggag aaaaattttg atggggcgaa aaagcattac caggacatat 180
cttcgtggaa atgctgttcc tgcgtatgtg ataataatcc ttgtaggaat tggtcaaatc 240
atcctgggag ggatattgta cgttgcattg aggaagaaga tcattgctgc acctgtaacg 300
gcatcatatg cagtggctag acaagaacca 330
<210> 296
<211> 197
<212> DNA
<213> 玉米根萤叶甲属
<400> 296
gtgtgtattt cagctggaat ttgtaatgaa aaaaacgtag aaatatatac tacaatgaag 60
tttttaagat cgacagtgtg ctacattgcc atcttggcaa ttctctttac cctctgtgcc 120
gatgaggttg aaggaaggag aaaaattttg atggggcgaa aaagcattac caggacatat 180
cttcgtggaa atgctgt 197
<210> 297
<211> 179
<212> DNA
<213> 玉米根萤叶甲属
<400> 297
atggggcgaa aaagcattac caggacatat cttcgtggaa atgctgttcc tgcgtatgtg 60
ataataatcc ttgtaggaat tggtcaaatc atcctgggag ggatattgta cgttgcattg 120
aggaagaaga tcattgctgc acctgtaacg gcatcatatg cagtggctag acaagaacc 179
<210> 298
<211> 478
<212> RNA
<213> 玉米根萤叶甲属
<400> 298
gguuucauga ccccugagag aaagaagaaa cuuagguuac uguugagaaa gaaagccgcc 60
gaagaauuaa agaaagaaca agaacgcaaa gcagccgaaa ggaggcguau cauugaagaa 120
aggugcggua aacccaaacu ugucgaugac gcaaaugaag gcccauuaaa acaaguaugu 180
gagggauauc acagacguau uguagaccua gaaaauaaga aauuugaccu cgaaaaagaa 240
guggaauuca gagauuuuca gaucuccgaa uugaacagcc aaguaaacga ccuuagaggc 300
aaauucguca aaccaaccuu gaagaaggua uccaaauacg aaaacaaauu cgccaaacuu 360
caaaagaagg cagcugaauu uaacuuccgu aaccaacuca aaguugucaa gaagaaagaa 420
uucaccuuag aagaagaaga caaagaaaag aaaccagacu ggucaaagaa gggagacg 478
<210> 299
<211> 413
<212> RNA
<213> 玉米根萤叶甲属
<400> 299
auggcggacg augagagaaa gaaacuggag gaggaaaaga agaggaaaca ggccgaaauu 60
gaacgcaaaa gggccgaggu cagggcucgu auggaagagg ccucaaaagc caagaaggcc 120
aagaaagguu ucaugacccc ugagagaaag aagaaacuua gguuacuguu gagaaagaaa 180
gccgccgaag aauuaaagaa agaacaagaa cgcaaagcag ccgaaaggag gcguaucauu 240
gaagaaaggu gcgguaaacc caaacuuguc gaugacgcaa augaaggccc auuaaaacaa 300
guaugugagg gauaucacag acguauugua gaccuagaaa auaagaaauu ugaccucgaa 360
aaagaagugg aauucagaga uuuucagauc uccgaauuga acagccaagu aaa 413
<210> 300
<211> 100
<212> RNA
<213> 玉米根萤叶甲属
<400> 300
agaucuccga auugaacagc caaguaaacg accuuagagg caaauucguc aaaccaaccu 60
ugaagaaggu auccaaauac gaaaacaaau ucgccaaacu 100
<210> 301
<211> 100
<212> RNA
<213> 玉米根萤叶甲属
<400> 301
ucaaaagaag gcagcugaau uuaacuuccg uaaccaacuc aaaguuguca agaagaaaga 60
auucaccuua gaagaagaag acaaagaaaa gaaaccagac 100
<210> 302
<211> 133
<212> RNA
<213> 玉米根萤叶甲属
<400> 302
gaccuuagag gcaaauucgu caaaccaacc uugaagaagg uauccaaaua cgaaaacaaa 60
uucgccaaac uucaaaagaa ggcagcugaa uuuaacuucc guaaccaacu caaaguuguc 120
aagaagaaag aau 133
<210> 303
<211> 500
<212> RNA
<213> 玉米根萤叶甲属
<400> 303
uacuaggcga aagugccguc gguaagucga guuugguacu gagguucguc aaaggacagu 60
uccacgaaua ccaggagagu accauaggag cagcuuuccu uacacaaacc auaugccucg 120
acgauacaac uguuaaauuu gaaauuuggg acacagcggg ucaagaaagg uaccacaguu 180
uagcuccuau guacuauagg ggcgcacagg cagcuauagu cgucuacgac auaaccaauc 240
aagacacauu cggcagggcg aaaacguggg ugaaggaacu ucaaaggcag gccaguccga 300
cgaucgugau agcuuuggcc ggcaacaagc aagauuuggc caacaaacgu augguagaau 360
acgaagaggc gcagacguau gcugacgaaa acggcuuacu uuuuauggaa acuuccgcaa 420
agacggcaau gaacgucaac gauauauuuu uagcaauagc uaagaaacug cccaagaaug 480
aacaaaccac aggucaaggc 500
<210> 304
<211> 648
<212> RNA
<213> 玉米根萤叶甲属
<400> 304
auggcggcaa acagaacugg accugcucag agaccaaaug gcgcuaccca aggaaagaua 60
ugucaguuca aacugguccu acuaggcgaa agugccgucg guaagucgag uuugguacug 120
agguucguca aaggacaguu ccacgaauac caggagagua ccauaggagc agcuuuccuu 180
acacaaacca uaugccucga cgauacaacu guuaaauuug aaauuuggga cacagcgggu 240
caagaaaggu accacaguuu agcuccuaug uacuauaggg gcgcacaggc agcuauaguc 300
gucuacgaca uaaccaauca agacacauuc ggcagggcga aaacgugggu gaaggaacuu 360
caaaggcagg ccaguccgac gaucgugaua gcuuuggccg gcaacaagca agauuuggcc 420
aacaaacgua ugguagaaua cgaagaggcg cagacguaug cugacgaaaa cggcuuacuu 480
uuuauggaaa cuuccgcaaa gacggcaaug aacgucaacg auauauuuuu agcaauagcu 540
aagaaacugc ccaagaauga acaaaccaca ggucaaggcg gcagugccca aggcaggcgg 600
cuagcggagg gcgauucggg cgccaaggca cccggaaauu guugcaag 648
<210> 305
<211> 188
<212> RNA
<213> 玉米根萤叶甲属
<400> 305
auggcggcaa acagaacugg accugcucag agaccaaaug gcgcuaccca aggaaagaua 60
ugucaguuca aacugguccu acuaggcgaa agugccgucg guaagucgag uuugguacug 120
agguucguca aaggacaguu ccacgaauac caggagagua ccauaggagc agcuuuccuu 180
acacaaac 188
<210> 306
<211> 205
<212> RNA
<213> 玉米根萤叶甲属
<400> 306
ggacaguucc acgaauacca ggagaguacc auaggagcag cuuuccuuac acaaaccaua 60
ugccucgacg auacaacugu uaaauuugaa auuugggaca cagcggguca agaaagguac 120
cacaguuuag cuccuaugua cuauaggggc gcacaggcag cuauagucgu cuacgacaua 180
accaaucaag acacauucgg caggg 205
<210> 307
<211> 224
<212> RNA
<213> 玉米根萤叶甲属
<400> 307
gcacaggcag cuauagucgu cuacgacaua accaaucaag acacauucgg cagggcgaaa 60
acguggguga aggaacuuca aaggcaggcc aguccgacga ucgugauagc uuuggccggc 120
aacaagcaag auuuggccaa caaacguaug guagaauacg aagaggcgca gacguaugcu 180
gacgaaaacg gcuuacuuuu uauggaaacu uccgcaaaga cggc 224
<210> 308
<211> 196
<212> RNA
<213> 玉米根萤叶甲属
<400> 308
gacguaugcu gacgaaaacg gcuuacuuuu uauggaaacu uccgcaaaga cggcaaugaa 60
cgucaacgau auauuuuuag caauagcuaa gaaacugccc aagaaugaac aaaccacagg 120
ucaaggcggc agugcccaag gcaggcggcu agcggagggc gauucgggcg ccaaggcacc 180
cggaaauugu ugcaag 196
<210> 309
<211> 189
<212> RNA
<213> 玉米根萤叶甲属
<400> 309
cgaugagguu gaaggaagga gaaaaauuuu gauggggcga aaaagcauua ccaggacaua 60
ucuucgugga aaugcuguuc cugcguaugu gauaauaauc cuuguaggaa uuggucaaau 120
cauccuggga gggauauugu acguugcauu gaggaagaag aucauugcug caccuguaac 180
ggcaucaua 189
<210> 310
<211> 330
<212> RNA
<213> 玉米根萤叶甲属
<400> 310
guguguauuu cagcuggaau uuguaaugaa aaaaacguag aaauauauac uacaaugaag 60
uuuuuaagau cgacagugug cuacauugcc aucuuggcaa uucucuuuac ccucugugcc 120
gaugagguug aaggaaggag aaaaauuuug auggggcgaa aaagcauuac caggacauau 180
cuucguggaa augcuguucc ugcguaugug auaauaaucc uuguaggaau uggucaaauc 240
auccugggag ggauauugua cguugcauug aggaagaaga ucauugcugc accuguaacg 300
gcaucauaug caguggcuag acaagaacca 330
<210> 311
<211> 197
<212> RNA
<213> 玉米根萤叶甲属
<400> 311
guguguauuu cagcuggaau uuguaaugaa aaaaacguag aaauauauac uacaaugaag 60
uuuuuaagau cgacagugug cuacauugcc aucuuggcaa uucucuuuac ccucugugcc 120
gaugagguug aaggaaggag aaaaauuuug auggggcgaa aaagcauuac caggacauau 180
cuucguggaa augcugu 197
<210> 312
<211> 179
<212> RNA
<213> 玉米根萤叶甲属
<400> 312
auggggcgaa aaagcauuac caggacauau cuucguggaa augcuguucc ugcguaugug 60
auaauaaucc uuguaggaau uggucaaauc auccugggag ggauauugua cguugcauug 120
aggaagaaga ucauugcugc accuguaacg gcaucauaug caguggcuag acaagaacc 179
<210> 313
<211> 44
<212> DNA
<213> 玉米根萤叶甲属
<400> 313
gaccttagag gcaaattcgt caaaccaacc ttgaagaagg tatc 44
<210> 314
<211> 45
<212> DNA
<213> 玉米根萤叶甲属
<400> 314
caaatacgaa aacaaattcg ccaaacttca aaagaaggca gctga 45
<210> 315
<211> 44
<212> DNA
<213> 玉米根萤叶甲属
<400> 315
atttaacttc cgtaaccaac tcaaagttgt caagaagaaa gaat 44
<210> 316
<211> 42
<212> DNA
<213> 玉米根萤叶甲属
<400> 316
accaaccttg aagaaggtat ccaaatacga aaacaaattc gc 42
<210> 317
<211> 46
<212> DNA
<213> 玉米根萤叶甲属
<400> 317
caaacttcaa aagaaggcag ctgaatttaa cttccgtaac caactc 46
<210> 318
<211> 133
<212> DNA
<213> 玉米根萤叶甲属
<400> 318
gaccttagag gcaaattcgt caaaccaacc ttgaagaagg tatcgacatg gtgagcaagg 60
gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 120
gccacaagtt cag 133
<210> 319
<211> 134
<212> DNA
<213> 玉米根萤叶甲属
<400> 319
caaatacgaa aacaaattcg ccaaacttca aaagaaggca gctgagacat ggtgagcaag 60
ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac 120
ggccacaagt tcag 134
<210> 320
<211> 133
<212> DNA
<213> 玉米根萤叶甲属
<400> 320
atttaacttc cgtaaccaac tcaaagttgt caagaagaaa gaatgacatg gtgagcaagg 60
gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 120
gccacaagtt cag 133
<210> 321
<211> 131
<212> DNA
<213> 玉米根萤叶甲属
<400> 321
accaaccttg aagaaggtat ccaaatacga aaacaaattc gcgacatggt gagcaagggc 60
gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc 120
cacaagttca g 131
<210> 322
<211> 135
<212> DNA
<213> 玉米根萤叶甲属
<400> 322
caaacttcaa aagaaggcag ctgaatttaa cttccgtaac caactcgaca tggtgagcaa 60
gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 120
cggccacaag ttcag 135
<210> 323
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 323
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gaccaacctt gaagaaggta tcagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 324
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 324
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gccaaccttg aagaaggtat ccagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 325
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 325
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gcaaccttga agaaggtatc caagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 326
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 326
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gaaccttgaa gaaggtatcc aaagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 327
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 327
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gaccttgaag aaggtatcca atagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 328
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 328
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gccttgaaga aggtatccaa taagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 329
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 329
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gcttgaagaa ggtatccaat acagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 330
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 330
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gttgaagaag gtatccaata cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 331
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 331
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gtgaagaagg tatccaatac gaagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 332
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 332
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
ggaagaaggt atccaatacg aaagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 333
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 333
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gaagaaggta tccaatacga aaagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 334
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 334
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gagaaggtat ccaatacgaa aaagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 335
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 335
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
ggaaggtatc caatacgaaa acagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 336
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 336
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gaaggtatcc aatacgaaaa caagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 337
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 337
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gaggtatcca atacgaaaac aaagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 338
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 338
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gggtatccaa tacgaaaaca aaagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 339
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 339
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
ggtatccaat acgaaaacaa atagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 340
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 340
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gtatccaata cgaaaacaaa ttagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 341
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 341
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gatccaatac gaaaacaaat tcagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 342
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 342
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gtccaatacg aaaacaaatt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 343
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 343
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gccaaatacg aaaacaaatt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 344
<211> 145
<212> DNA
<213> 玉米根萤叶甲属
<400> 344
ggatccgaca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 60
gcaaatacga aaacaaattc gcagctggac ggcgacgtaa acggccacaa gttcagcgtg 120
tccggcgagg gcgaggggcg gccgc 145
<210> 345
<211> 44
<212> RNA
<213> 玉米根萤叶甲属
<400> 345
gaccuuagag gcaaauucgu caaaccaacc uugaagaagg uauc 44
<210> 346
<211> 45
<212> RNA
<213> 玉米根萤叶甲属
<400> 346
caaauacgaa aacaaauucg ccaaacuuca aaagaaggca gcuga 45
<210> 347
<211> 44
<212> RNA
<213> 玉米根萤叶甲属
<400> 347
auuuaacuuc cguaaccaac ucaaaguugu caagaagaaa gaau 44
<210> 348
<211> 42
<212> RNA
<213> 玉米根萤叶甲属
<400> 348
accaaccuug aagaagguau ccaaauacga aaacaaauuc gc 42
<210> 349
<211> 46
<212> RNA
<213> 玉米根萤叶甲属
<400> 349
caaacuucaa aagaaggcag cugaauuuaa cuuccguaac caacuc 46
<210> 350
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 350
accaaccuug aagaagguau c 21
<210> 351
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 351
ccaaccuuga agaagguauc c 21
<210> 352
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 352
caaccuugaa gaagguaucc a 21
<210> 353
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 353
aaccuugaag aagguaucca a 21
<210> 354
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 354
accuugaaga agguauccaa u 21
<210> 355
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 355
ccuugaagaa gguauccaau a 21
<210> 356
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 356
cuugaagaag guauccaaua c 21
<210> 357
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 357
uugaagaagg uauccaauac g 21
<210> 358
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 358
ugaagaaggu auccaauacg a 21
<210> 359
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 359
gaagaaggua uccaauacga a 21
<210> 360
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 360
aagaagguau ccaauacgaa a 21
<210> 361
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 361
agaagguauc caauacgaaa a 21
<210> 362
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 362
gaagguaucc aauacgaaaa c 21
<210> 363
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 363
aagguaucca auacgaaaac a 21
<210> 364
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 364
agguauccaa uacgaaaaca a 21
<210> 365
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 365
gguauccaau acgaaaacaa a 21
<210> 366
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 366
guauccaaua cgaaaacaaa u 21
<210> 367
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 367
uauccaauac gaaaacaaau u 21
<210> 368
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 368
auccaauacg aaaacaaauu c 21
<210> 369
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 369
uccaauacga aaacaaauuc g 21
<210> 370
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 370
ccaaauacga aaacaaauuc g 21
<210> 371
<211> 21
<212> RNA
<213> 玉米根萤叶甲属
<400> 371
caaauacgaa aacaaauucg c 21
<210> 372
<211> 5055
<212> DNA
<213> 玉米根萤叶甲属
<400> 372
atgacgcagc aactgttacc tatcaagttc caagaacatt tgcagcttac caatgttggt 60
atccatgcta ccaacatcac gttcgccaat ctcaccatgg agagcgacaa gtacatctgc 120
gtgagagaaa aagttggaga gacgtcccaa gtagtcatca tagatatggc cgatacggcc 180
aatcccatca gacgaccaat caccgccgaa agtgctatta tgaatccagc atccaaagtc 240
atcgctctta aaggtaaagc aggcgtagaa acacaaaaaa cccttcaaat attcaacata 300
gaaatgaagt caaaaatgaa agcccacacc atgtctgaag acgtaatttt ctggaaatgg 360
atcagcccca atacgttagc gctagtcact gaaacttcag tgtatcattg gtccatggaa 420
ggtgattcaa ttccacagaa aatgtttgac aggcattctt ctcttaacgg ctgccaaatc 480
atcaactacc gaacagatcc caaacaaaac tggctgctac tggtcggtat tagcgctcag 540
caatctcgcg tcgttggtgc tatgcaactg tactcagtag aaagaaaatg ctcgcagcca 600
atcgaaggtc acgccgcttc tttcgcgacc ttcaaaatgg aaggaaaccc tgaaccatcg 660
actctcttct gttttgcagt gaggacttta caaggaggaa agttacatat tatcgaggtt 720
ggtcaaagtc cagcaggcaa ccaatcgttt cctaaaaaga cagtggatgt tttcttccca 780
cccgaggccc aaaatgattt tcctgtagct atgcaagtgt ccgctaaata cgacgtcatt 840
tacctgatta ccaaatacgg ctatattcat atgtatgaca tcgagagcgg cacctgtatc 900
tacatgaaca gaatatctag tgacacaata ttcgtaaccg caccccacga agctacggga 960
ggaatcattg gtgttaatag aaagggtcaa gtcctatctg tctcggtaga tgaggagagc 1020
attattcgat acatcaacac cgtcttacac aatgctgact tagcgctgcg tctagctacg 1080
agaaacaatc tttcaggagc cgaagaactg tttgttagta aattccagat gttgttccag 1140
aacggacagt acgccgaagc tgctaaagtg gctgccaacg ctccaaaagg aattttaaga 1200
accccagcta caattcaaat gtttcaacaa gtcccgactc aacctggaca aaacagtccc 1260
cttttgcaat atttcggtat acttctagac caagggcaac taaacagata cgaatctttg 1320
gaattgtgta aaccagtttt gttgcaaggt cgcaaacagc ttctagaaaa atggttaaaa 1380
gaggaaaaat tagaatgttc cgaagaatta ggagacctag tcaaacaggc agatcctact 1440
ctggctttat ctgtgtatct tagagctaat gtacctgcaa aagttatcca aagctttgcc 1500
gaaactggcc aattccaaaa gatagtcctc tacgccaaaa aagtgtctta tactcccgac 1560
tatgtattcc tgcttcgcca agtcatgcgc accaatcccg accaaggcgc agcatttgca 1620
gggatgttgg tagcggacga cgaacctttg gccgatatca accaaatcgt ggacattttc 1680
atggagcaaa acatggtgca gcagtgtact gcgttcttgt tggacgcctt aaagaataac 1740
aggcctactg aggggcatct acaaactaga ctattagaaa tgaacctgat gtccgcaccg 1800
caagtagcag acgccattct cggcaacaac atgttcactc actacgaccg gccccacgta 1860
gcccagctct gcgaaaaagc tggtctttta caaagagctc tagaacacta cactgatttg 1920
tatgacatca aacgtgctgt ggtacacacc cacttgcttc cagctgattg gttggttaac 1980
ttcttcggaa ctctcagcgt ggaagacagt ttggagtgct tgagggccat gctgaccgcg 2040
aatattcgac agaatttgca gatttgcgtg cagatcgcta ccaagtacca cgaacaactc 2100
accactaagg ctttgatcga tttgtttgaa ggatttaaga gctatgaggg tttgttctat 2160
ttcctcggct ccatcgtcaa cttctcccaa gaccaagaag tgcacttcaa gtacatccaa 2220
gccgcatgca agactggcca aatcaaagaa gtcgagcgta tctgtaggga atcaaactgc 2280
tacaatcccg aaagagtcaa gaatttcctg aaggaagcca aacttacaga tcagttgccg 2340
ttgattattg tctgtgacag atttgatttc gtccacgact tagtgttgta tctatataga 2400
aattcgctgc agaaatatat cgagatttat gtccagaagg tcaatcccag ccgtttaccg 2460
gttgtggtag gaggtctttt agatgtcgac tgttccgaag acataatcaa aaacctaatt 2520
cttgtcgtca gaggccagtt ctccactgac gaattagtag aagaagtaga aaagaggaac 2580
agattgaaac tgttgttgcc ctggttggag agcagagtac acgaaggatg tgtcgagcca 2640
gctacgcaca atgctttggc caagatctac atcgattcca acaacaatgc cgaaagattc 2700
ttgaaggaaa atcaatggta cgattcccga gttgtgggac gttattgcga aaagcgcgat 2760
ccacatctgg cttgcgttgc ttacgaacga ggccaatgcg acagagaact gatagctgta 2820
tgtaacgaaa actctctatt taagtctgaa gctcgttatt tggtccgtag acgtgacgga 2880
gaattatggg ctgaggtttt gaacgagagc aatccttaca gacgtcagtt gatagaccaa 2940
gtagtgcaaa cagctttaag tgaaacccaa gaccccgaag atatctctgt taccgttaaa 3000
gcattcatga cagctgattt accaaatgag ctcattgaat tgttggaaaa gattgtattg 3060
gatagttctg tgttctccga gcatagaaac cttcaaaatt tgcttatcct aacagcaatc 3120
aaagccgatg ctacaagagt tatggactac atcaaccgct tggataacta cgatgcccct 3180
gatatcgcga atatagccat caacaaccat ctctatgaag aagctttcgc tattttcaag 3240
aaatttgatg tcaacacctc agctattcaa gtattgatcg aacaagtcaa caatctggat 3300
cgtgcttatg aattcgcaga acgttgcaat gaaccggccg tatggagtca gctggccaaa 3360
gcgcaactga accaaggctt agtcaaggaa gcaatcgatt cttacattaa agctgatgat 3420
ccttcagctt ataaggacgt cgttgagacc gcttcgaaaa ataatagctg ggaggactta 3480
gtgcggtatt tgcagatggc aagaaagaag gccagagaga gctacatcga atctgaattg 3540
atttattcgt acgcgaagac cggaagattg gctgatttgg aagaattcat cagtggaccc 3600
aaccatgctg atatccagaa gattggtgat aggtgctttg atgacaagat gtatgatgct 3660
gctaagctac tctacaacaa tgtatccaac ttcgctcgtc tcgctattac tttagtgcat 3720
ctaaaagaat tccagggagc tgttgacagt gctaggaagg ctaatagtac cagaacatgg 3780
aaggaagtgt gttttgcttg cgtcgatgcc gaagaattca gattggctca gatgtgtggt 3840
atgcacatcg tggtacacgc tgatgagttg caagatttga ttaattatta tcaagataga 3900
ggatactttg aagaattaat cggcctattg gaagcagcat tgggcttgga aagggcacac 3960
atgggtatgt tcaccgaatt agcaattttg tattccaaat ataagcccgc caaaatgcgc 4020
gaacatctgg aactcttctg gtctcgagtg aatatcccca aagttcttag ggcagctgaa 4080
caagcacatt tgtgggcaga gctagtgttt ttgtatgaca aatatgaaga atatgataac 4140
gctgtgttag ccatgatggc tcatcccacc gaagcttggc gcgaaggtca tttcaaggat 4200
attattacaa aagttgccaa tatcgaactt tattatagag ctattcaatt ttacttggat 4260
tacaaaccgc tgttgttaaa tgaccttttg ttggtgttgg cacccaggat ggatcatact 4320
agggctgttt ctttctttac aaaaacagga cacttacagt tggttaaatc ctatctccgg 4380
tctgtgcaaa atttgaacaa caaagctatc aacgaggccc tcaactctct acttatcgaa 4440
gaagaagact tccagggtct gagaacgtcc atcgacgcct tcgataactt cgacaacatc 4500
ggcttggctc agaaattgga gaagcacgaa ctgacggagt tcagacgcat cgccgcctac 4560
ttgtacaaag gcaacaaccg gtggaagcag agcgtagaac tgtgcaaaaa ggatagacta 4620
tttagagacg ctatggagta cacttctgaa tctagaaatc aagaattggc cgaagagttg 4680
ctggcatggt tcttggatag gaaggcctat gattgttttt cggcatgttt gtatcactgc 4740
tacgacttgt tacggcccga cgttatcctt gaactagcat ggaaacacaa cattatggac 4800
ttcgcaatgc ctttccttat tcaagtaact agagaactga cgacaaaagt agaaaagcta 4860
gaacaatcag atgcccaacg acaaagcgag gctgctgaag aaacgaacaa gccaatgatg 4920
ataccagaac cccaacttat gctaacggct ggccctggca tgggtattcc accccaacag 4980
tatgtacctc cccaaggcta cgcgcagcca ggctacgccc cgcaaatggc ttaccaggga 5040
tacccaggca tgtaa 5055
<210> 373
<211> 5055
<212> RNA
<213> 玉米根萤叶甲属
<400> 373
augacgcagc aacuguuacc uaucaaguuc caagaacauu ugcagcuuac caauguuggu 60
auccaugcua ccaacaucac guucgccaau cucaccaugg agagcgacaa guacaucugc 120
gugagagaaa aaguuggaga gacgucccaa guagucauca uagauauggc cgauacggcc 180
aaucccauca gacgaccaau caccgccgaa agugcuauua ugaauccagc auccaaaguc 240
aucgcucuua aagguaaagc aggcguagaa acacaaaaaa cccuucaaau auucaacaua 300
gaaaugaagu caaaaaugaa agcccacacc augucugaag acguaauuuu cuggaaaugg 360
aucagcccca auacguuagc gcuagucacu gaaacuucag uguaucauug guccauggaa 420
ggugauucaa uuccacagaa aauguuugac aggcauucuu cucuuaacgg cugccaaauc 480
aucaacuacc gaacagaucc caaacaaaac uggcugcuac uggucgguau uagcgcucag 540
caaucucgcg ucguuggugc uaugcaacug uacucaguag aaagaaaaug cucgcagcca 600
aucgaagguc acgccgcuuc uuucgcgacc uucaaaaugg aaggaaaccc ugaaccaucg 660
acucucuucu guuuugcagu gaggacuuua caaggaggaa aguuacauau uaucgagguu 720
ggucaaaguc cagcaggcaa ccaaucguuu ccuaaaaaga caguggaugu uuucuuccca 780
cccgaggccc aaaaugauuu uccuguagcu augcaagugu ccgcuaaaua cgacgucauu 840
uaccugauua ccaaauacgg cuauauucau auguaugaca ucgagagcgg caccuguauc 900
uacaugaaca gaauaucuag ugacacaaua uucguaaccg caccccacga agcuacggga 960
ggaaucauug guguuaauag aaagggucaa guccuaucug ucucgguaga ugaggagagc 1020
auuauucgau acaucaacac cgucuuacac aaugcugacu uagcgcugcg ucuagcuacg 1080
agaaacaauc uuucaggagc cgaagaacug uuuguuagua aauuccagau guuguuccag 1140
aacggacagu acgccgaagc ugcuaaagug gcugccaacg cuccaaaagg aauuuuaaga 1200
accccagcua caauucaaau guuucaacaa gucccgacuc aaccuggaca aaacaguccc 1260
cuuuugcaau auuucgguau acuucuagac caagggcaac uaaacagaua cgaaucuuug 1320
gaauugugua aaccaguuuu guugcaaggu cgcaaacagc uucuagaaaa augguuaaaa 1380
gaggaaaaau uagaauguuc cgaagaauua ggagaccuag ucaaacaggc agauccuacu 1440
cuggcuuuau cuguguaucu uagagcuaau guaccugcaa aaguuaucca aagcuuugcc 1500
gaaacuggcc aauuccaaaa gauaguccuc uacgccaaaa aagugucuua uacucccgac 1560
uauguauucc ugcuucgcca agucaugcgc accaaucccg accaaggcgc agcauuugca 1620
gggauguugg uagcggacga cgaaccuuug gccgauauca accaaaucgu ggacauuuuc 1680
auggagcaaa acauggugca gcaguguacu gcguucuugu uggacgccuu aaagaauaac 1740
aggccuacug aggggcaucu acaaacuaga cuauuagaaa ugaaccugau guccgcaccg 1800
caaguagcag acgccauucu cggcaacaac auguucacuc acuacgaccg gccccacgua 1860
gcccagcucu gcgaaaaagc uggucuuuua caaagagcuc uagaacacua cacugauuug 1920
uaugacauca aacgugcugu gguacacacc cacuugcuuc cagcugauug guugguuaac 1980
uucuucggaa cucucagcgu ggaagacagu uuggagugcu ugagggccau gcugaccgcg 2040
aauauucgac agaauuugca gauuugcgug cagaucgcua ccaaguacca cgaacaacuc 2100
accacuaagg cuuugaucga uuuguuugaa ggauuuaaga gcuaugaggg uuuguucuau 2160
uuccucggcu ccaucgucaa cuucucccaa gaccaagaag ugcacuucaa guacauccaa 2220
gccgcaugca agacuggcca aaucaaagaa gucgagcgua ucuguaggga aucaaacugc 2280
uacaaucccg aaagagucaa gaauuuccug aaggaagcca aacuuacaga ucaguugccg 2340
uugauuauug ucugugacag auuugauuuc guccacgacu uaguguugua ucuauauaga 2400
aauucgcugc agaaauauau cgagauuuau guccagaagg ucaaucccag ccguuuaccg 2460
guugugguag gaggucuuuu agaugucgac uguuccgaag acauaaucaa aaaccuaauu 2520
cuugucguca gaggccaguu cuccacugac gaauuaguag aagaaguaga aaagaggaac 2580
agauugaaac uguuguugcc cugguuggag agcagaguac acgaaggaug ugucgagcca 2640
gcuacgcaca augcuuuggc caagaucuac aucgauucca acaacaaugc cgaaagauuc 2700
uugaaggaaa aucaauggua cgauucccga guugugggac guuauugcga aaagcgcgau 2760
ccacaucugg cuugcguugc uuacgaacga ggccaaugcg acagagaacu gauagcugua 2820
uguaacgaaa acucucuauu uaagucugaa gcucguuauu ugguccguag acgugacgga 2880
gaauuauggg cugagguuuu gaacgagagc aauccuuaca gacgucaguu gauagaccaa 2940
guagugcaaa cagcuuuaag ugaaacccaa gaccccgaag auaucucugu uaccguuaaa 3000
gcauucauga cagcugauuu accaaaugag cucauugaau uguuggaaaa gauuguauug 3060
gauaguucug uguucuccga gcauagaaac cuucaaaauu ugcuuauccu aacagcaauc 3120
aaagccgaug cuacaagagu uauggacuac aucaaccgcu uggauaacua cgaugccccu 3180
gauaucgcga auauagccau caacaaccau cucuaugaag aagcuuucgc uauuuucaag 3240
aaauuugaug ucaacaccuc agcuauucaa guauugaucg aacaagucaa caaucuggau 3300
cgugcuuaug aauucgcaga acguugcaau gaaccggccg uauggaguca gcuggccaaa 3360
gcgcaacuga accaaggcuu agucaaggaa gcaaucgauu cuuacauuaa agcugaugau 3420
ccuucagcuu auaaggacgu cguugagacc gcuucgaaaa auaauagcug ggaggacuua 3480
gugcgguauu ugcagauggc aagaaagaag gccagagaga gcuacaucga aucugaauug 3540
auuuauucgu acgcgaagac cggaagauug gcugauuugg aagaauucau caguggaccc 3600
aaccaugcug auauccagaa gauuggugau aggugcuuug augacaagau guaugaugcu 3660
gcuaagcuac ucuacaacaa uguauccaac uucgcucguc ucgcuauuac uuuagugcau 3720
cuaaaagaau uccagggagc uguugacagu gcuaggaagg cuaauaguac cagaacaugg 3780
aaggaagugu guuuugcuug cgucgaugcc gaagaauuca gauuggcuca gauguguggu 3840
augcacaucg ugguacacgc ugaugaguug caagauuuga uuaauuauua ucaagauaga 3900
ggauacuuug aagaauuaau cggccuauug gaagcagcau ugggcuugga aagggcacac 3960
auggguaugu ucaccgaauu agcaauuuug uauuccaaau auaagcccgc caaaaugcgc 4020
gaacaucugg aacucuucug gucucgagug aauaucccca aaguucuuag ggcagcugaa 4080
caagcacauu ugugggcaga gcuaguguuu uuguaugaca aauaugaaga auaugauaac 4140
gcuguguuag ccaugauggc ucaucccacc gaagcuuggc gcgaagguca uuucaaggau 4200
auuauuacaa aaguugccaa uaucgaacuu uauuauagag cuauucaauu uuacuuggau 4260
uacaaaccgc uguuguuaaa ugaccuuuug uugguguugg cacccaggau ggaucauacu 4320
agggcuguuu cuuucuuuac aaaaacagga cacuuacagu ugguuaaauc cuaucuccgg 4380
ucugugcaaa auuugaacaa caaagcuauc aacgaggccc ucaacucucu acuuaucgaa 4440
gaagaagacu uccagggucu gagaacgucc aucgacgccu ucgauaacuu cgacaacauc 4500
ggcuuggcuc agaaauugga gaagcacgaa cugacggagu ucagacgcau cgccgccuac 4560
uuguacaaag gcaacaaccg guggaagcag agcguagaac ugugcaaaaa ggauagacua 4620
uuuagagacg cuauggagua cacuucugaa ucuagaaauc aagaauuggc cgaagaguug 4680
cuggcauggu ucuuggauag gaaggccuau gauuguuuuu cggcauguuu guaucacugc 4740
uacgacuugu uacggcccga cguuauccuu gaacuagcau ggaaacacaa cauuauggac 4800
uucgcaaugc cuuuccuuau ucaaguaacu agagaacuga cgacaaaagu agaaaagcua 4860
gaacaaucag augcccaacg acaaagcgag gcugcugaag aaacgaacaa gccaaugaug 4920
auaccagaac cccaacuuau gcuaacggcu ggcccuggca uggguauucc accccaacag 4980
uauguaccuc cccaaggcua cgcgcagcca ggcuacgccc cgcaaauggc uuaccaggga 5040
uacccaggca uguaa 5055
<210> 374
<211> 7132
<212> RNA
<213> 玉米根萤叶甲属
<400> 374
ccggccugua caaaagggcg acgccauauu agauguuuga uucagcuagc ugucaguuua 60
gugaaaaaau ugucauuuuc cuuaaauuuc cgaaaacguu ucaauaagua aaguggaaag 120
uguuuaaauu uuauuuugug uuuuguugca aagaaaaagc aacaugacgc agcaacuguu 180
accuaucaag uuccaagaac auuugcagcu uaccaauguu gguauccaug cuaccaacau 240
cacguucgcc aaucucacca uggagagcga caaguacauc ugcgugagag aaaaaguugg 300
agagacgucc caaguaguca ucauagauau ggccgauacg gccaauccca ucagacgacc 360
aaucaccgcc gaaagugcua uuaugaaucc agcauccaaa gucaucgcuc uuaaagguaa 420
agcaggcgua gaaacacaaa aaacccuuca aauauucaac auagaaauga agucaaaaau 480
gaaagcccac accaugucug aagacguaau uuucuggaaa uggaucagcc ccaauacguu 540
agcgcuaguc acugaaacuu caguguauca uugguccaug gaaggugauu caauuccaca 600
gaaaauguuu gacaggcauu cuucucuuaa cggcugccaa aucaucaacu accgaacaga 660
ucccaaacaa aacuggcugc uacuggucgg uauuagcgcu cagcaaucuc gcgucguugg 720
ugcuaugcaa cuguacucag uagaaagaaa augcucgcag ccaaucgaag gucacgccgc 780
uucuuucgcg accuucaaaa uggaaggaaa cccugaacca ucgacucucu ucuguuuugc 840
agugaggacu uuacaaggag gaaaguuaca uauuaucgag guuggucaaa guccagcagg 900
caaccaaucg uuuccuaaaa agacagugga uguuuucuuc ccacccgagg cccaaaauga 960
uuuuccugua gcuaugcaag uguccgcuaa auacgacguc auuuaccuga uuaccaaaua 1020
cggcuauauu cauauguaug acaucgagag cggcaccugu aucuacauga acagaauauc 1080
uagugacaca auauucguaa ccgcacccca cgaagcuacg ggaggaauca uugguguuaa 1140
uagaaagggu caaguccuau cugucucggu agaugaggag agcauuauuc gauacaucaa 1200
caccgucuua cacaaugcug acuuagcgcu gcgucuagcu acgagaaaca aucuuucagg 1260
agccgaagaa cuguuuguua guaaauucca gauguuguuc cagaacggac aguacgccga 1320
agcugcuaaa guggcugcca acgcuccaaa aggaauuuua agaaccccag cuacaauuca 1380
aauguuucaa caagucccga cucaaccugg acaaaacagu ccccuuuugc aauauuucgg 1440
uauacuucua gaccaagggc aacuaaacag auacgaaucu uuggaauugu guaaaccagu 1500
uuuguugcaa ggucgcaaac agcuucuaga aaaaugguua aaagaggaaa aauuagaaug 1560
uuccgaagaa uuaggagacc uagucaaaca ggcagauccu acucuggcuu uaucugugua 1620
ucuuagagcu aauguaccug caaaaguuau ccaaagcuuu gccgaaacug gccaauucca 1680
aaagauaguc cucuacgcca aaaaaguguc uuauacuccc gacuauguau uccugcuucg 1740
ccaagucaug cgcaccaauc ccgaccaagg cgcagcauuu gcagggaugu ugguagcgga 1800
cgacgaaccu uuggccgaua ucaaccaaau cguggacauu uucauggagc aaaacauggu 1860
gcagcagugu acugcguucu uguuggacgc cuuaaagaau aacaggccua cugaggggca 1920
ucuacaaacu agacuauuag aaaugaaccu gauguccgca ccgcaaguag cagacgccau 1980
ucucggcaac aacauguuca cucacuacga ccggccccac guagcccagc ucugcgaaaa 2040
agcuggucuu uuacaaagag cucuagaaca cuacacugau uuguaugaca ucaaacgugc 2100
ugugguacac acccacuugc uuccagcuga uugguugguu aacuucuucg gaacucucag 2160
cguggaagac aguuuggagu gcuugagggc caugcugacc gcgaauauuc gacagaauuu 2220
gcagauuugc gugcagaucg cuaccaagua ccacgaacaa cucaccacua aggcuuugau 2280
cgauuuguuu gaaggauuua agagcuauga ggguuuguuc uauuuccucg gcuccaucgu 2340
caacuucucc caagaccaag aagugcacuu caaguacauc caagccgcau gcaagacugg 2400
ccaaaucaaa gaagucgagc guaucuguag ggaaucaaac ugcuacaauc ccgaaagagu 2460
caagaauuuc cugaaggaag ccaaacuuac agaucaguug ccguugauua uugucuguga 2520
cagauuugau uucguccacg acuuaguguu guaucuauau agaaauucgc ugcagaaaua 2580
uaucgagauu uauguccaga aggucaaucc cagccguuua ccgguugugg uaggaggucu 2640
uuuagauguc gacuguuccg aagacauaau caaaaaccua auucuugucg ucagaggcca 2700
guucuccacu gacgaauuag uagaagaagu agaaaagagg aacagauuga aacuguuguu 2760
gcccugguug gagagcagag uacacgaagg augugucgag ccagcuacgc acaaugcuuu 2820
ggccaagauc uacaucgauu ccaacaacaa ugccgaaaga uucuugaagg aaaaucaaug 2880
guacgauucc cgaguugugg gacguuauug cgaaaagcgc gauccacauc uggcuugcgu 2940
ugcuuacgaa cgaggccaau gcgacagaga acugauagcu guauguaacg aaaacucucu 3000
auuuaagucu gaagcucguu auuugguccg uagacgugac ggagaauuau gggcugaggu 3060
uuugaacgag agcaauccuu acagacguca guugauagac caaguagugc aaacagcuuu 3120
aagugaaacc caagaccccg aagauaucuc uguuaccguu aaagcauuca ugacagcuga 3180
uuuaccaaau gagcucauug aauuguugga aaagauugua uuggauaguu cuguguucuc 3240
cgagcauaga aaccuucaaa auuugcuuau ccuaacagca aucaaagccg augcuacaag 3300
aguuauggac uacaucaacc gcuuggauaa cuacgaugcc ccugauaucg cgaauauagc 3360
caucaacaac caucucuaug aagaagcuuu cgcuauuuuc aagaaauuug augucaacac 3420
cucagcuauu caaguauuga ucgaacaagu caacaaucug gaucgugcuu augaauucgc 3480
agaacguugc aaugaaccgg ccguauggag ucagcuggcc aaagcgcaac ugaaccaagg 3540
cuuagucaag gaagcaaucg auucuuacau uaaagcugau gauccuucag cuuauaagga 3600
cgucguugag accgcuucga aaaauaauag cugggaggac uuagugcggu auuugcagau 3660
ggcaagaaag aaggccagag agagcuacau cgaaucugaa uugauuuauu cguacgcgaa 3720
gaccggaaga uuggcugauu uggaagaauu caucagugga cccaaccaug cugauaucca 3780
gaagauuggu gauaggugcu uugaugacaa gauguaugau gcugcuaagc uacucuacaa 3840
caauguaucc aacuucgcuc gucucgcuau uacuuuagug caucuaaaag aauuccaggg 3900
agcuguugac agugcuagga aggcuaauag uaccagaaca uggaaggaag uguguuuugc 3960
uugcgucgau gccgaagaau ucagauuggc ucagaugugu gguaugcaca ucgugguaca 4020
cgcugaugag uugcaagauu ugauuaauua uuaucaagau agaggauacu uugaagaauu 4080
aaucggccua uuggaagcag cauugggcuu ggaaagggca cacaugggua uguucaccga 4140
auuagcaauu uuguauucca aauauaagcc cgccaaaaug cgcgaacauc uggaacucuu 4200
cuggucucga gugaauaucc ccaaaguucu uagggcagcu gaacaagcac auuugugggc 4260
agagcuagug uuuuuguaug acaaauauga agaauaugau aacgcugugu uagccaugau 4320
ggcucauccc accgaagcuu ggcgcgaagg ucauuucaag gauauuauua caaaaguugc 4380
caauaucgaa cuuuauuaua gagcuauuca auuuuacuug gauuacaaac cgcuguuguu 4440
aaaugaccuu uuguuggugu uggcacccag gauggaucau acuagggcug uuucuuucuu 4500
uacaaaaaca ggacacuuac aguugguuaa auccuaucuc cggucugugc aaaauuugaa 4560
caacaaagcu aucaacgagg cccucaacuc ucuacuuauc gaagaagaag acuuccaggg 4620
ucugagaacg uccaucgacg ccuucgauaa cuucgacaac aucggcuugg cucagaaauu 4680
ggagaagcac gaacugacgg aguucagacg caucgccgcc uacuuguaca aaggcaacaa 4740
ccgguggaag cagagcguag aacugugcaa aaaggauaga cuauuuagag acgcuaugga 4800
guacacuucu gaaucuagaa aucaagaauu ggccgaagag uugcuggcau gguucuugga 4860
uaggaaggcc uaugauuguu uuucggcaug uuuguaucac ugcuacgacu uguuacggcc 4920
cgacguuauc cuugaacuag cauggaaaca caacauuaug gacuucgcaa ugccuuuccu 4980
uauucaagua acuagagaac ugacgacaaa aguagaaaag cuagaacaau cagaugccca 5040
acgacaaagc gaggcugcug aagaaacgaa caagccaaug augauaccag aaccccaacu 5100
uaugcuaacg gcuggcccug gcauggguau uccaccccaa caguauguac cuccccaagg 5160
cuacgcgcag ccaggcuacg ccccgcaaau ggcuuaccag ggauacccag gcauguaaua 5220
uaaggccccu uuuacagcca acuguauagg aaaguggaau uaguuuucaa guuaaacuga 5280
ggagcuagug cuaaggacaa uagagaaguu auaacuaaaa aaauagcggu uccaauucgu 5340
uucguguauu augaacaacg uuacuuucua aaagaagaag aauauauuga ggaguucguu 5400
uuaaaauuau guacaaguag aaguuuuaau acuugguuaa cuaaaaacgu uacauuuuuu 5460
ugagauuaca aauaacugaa uaauugaaaa uauuacuuac aauuuuaugc gaacugcucg 5520
auaaauuucu caaaaauagc aaaacaaaua auagguuuau ugaaguguua cagaaaauuu 5580
aggcauuucc uuuaaaucaa auaaauguuu cauuauuugu gugaaaaguu auuuauuaac 5640
auuauucauc uuuugcuaaa aaacaguuga gauuauugca aaauguuauu gcauuuugaa 5700
aguaaagaug uaaauguguu acaaaugauc uauuuguagu uuugucauca uuauguuuug 5760
uuuuuccguc cauuguugag uauauuucuu cauaaauaaa guaucagaug auguuuauau 5820
auaauacaau aauuuuuauu cuuuaaucgc uucaauauuu guuauaaugu cuucaauuuc 5880
aacauauugu uuuuuuuguc guauuggucu uuuuaguguu uccugccacc ucuaaccaau 5940
uauuaggcga uuuuacauga ggauuaagcc uacagauguc aaaauggcgu cauaucuauc 6000
gaaugacaac ugacaguuga agugucauau uuaaauuaug aucauacugu uccgucuuuc 6060
uucgucugua auucuaauuc guucauaacg aucuaccuac ucuuuucauu uuuuuuugug 6120
uaguuauuua ggcauuuuua agucuacgac ugucaaaaua gugucaacug acaaguugga 6180
agugucauac uuaaauuauc accauuuuau ccugacuccc uuugucuuca auuuugauuu 6240
cauaauaauu uuuauuucag ugcccacuug aaccuuuuuu uauaauuuac auaucucaua 6300
gucauuuuua ggcguuuaac uuacaacauu guuuccacac acauuuucca gcuucgauau 6360
caguuauuga uuuacaauaa uuuaaauuug cuccuuuaua caaauguaac aacccaauuc 6420
caacccuuuu auuaauuuuc uuuuuguuuu gauaagauuu auuugcaaca aacaauucua 6480
gcgaaggaag aauuuccaac aaguuaaaaa caaacuuauu ucaauuguca aaccuuuuaa 6540
uuuucuuucg aaaucuugau auuucuuucg cuagaauaau ugccccaaau ugaauuucag 6600
guacuuuccu ucgagucaag aucuggucau aaccccaaaa aagacaguug acgugucgug 6660
ucaugacacu gacguuugaa cacuucaauu gucaguuguc auggaagacc guugguagag 6720
auaaaaugac caaauuuucu guagcacauu auguaagcuu aaagcuuaaa gaauaaaugc 6780
uuaaacaaau ucuaguuaaa uacaaucaau cuuguugaac uguuuguugu aguuuuaauu 6840
cauucgcauc auuuucggac auuaggcuuc guuuugacgg gacacuugau uuacuuuuuc 6900
aguuggagaa uguauauaua uuuuuaugua cuagcacccc acuuuagaau guauauaauc 6960
uaaagaaauu auuuuguuuu ccgagagaau uaucaaugcg uucuuuuguu uguuuucuuu 7020
uuuuuagucc guuuaucauu ccuuuauuua aacauagaua uauguauaug aagugacuau 7080
uuaacuuaua uuauuauaug aauuauauga aagugauucu ucaauguuua au 7132
<210> 375
<211> 5055
<212> RNA
<213> 玉米根萤叶甲属
<400> 375
uacugcgucg uugacaaugg auaguucaag guucuuguaa acgucgaaug guuacaacca 60
uagguacgau gguuguagug caagcgguua gagugguacc ucucgcuguu cauguagacg 120
cacucucuuu uucaaccucu cugcaggguu caucaguagu aucuauaccg gcuaugccgg 180
uuaggguagu cugcugguua guggcggcuu ucacgauaau acuuaggucg uagguuucag 240
uagcgagaau uuccauuucg uccgcaucuu uguguuuuuu gggaaguuua uaaguuguau 300
cuuuacuuca guuuuuacuu ucgggugugg uacagacuuc ugcauuaaaa gaccuuuacc 360
uagucggggu uaugcaaucg cgaucaguga cuuugaaguc acauaguaac cagguaccuu 420
ccacuaaguu aaggugucuu uuacaaacug uccguaagaa gagaauugcc gacgguuuag 480
uaguugaugg cuugucuagg guuuguuuug accgacgaug accagccaua aucgcgaguc 540
guuagagcgc agcaaccacg auacguugac augagucauc uuucuuuuac gagcgucggu 600
uagcuuccag ugcggcgaag aaagcgcugg aaguuuuacc uuccuuuggg acuugguagc 660
ugagagaaga caaaacguca cuccugaaau guuccuccuu ucaauguaua auagcuccaa 720
ccaguuucag gucguccguu gguuagcaaa ggauuuuucu gucaccuaca aaagaagggu 780
gggcuccggg uuuuacuaaa aggacaucga uacguucaca ggcgauuuau gcugcaguaa 840
auggacuaau gguuuaugcc gauauaagua uacauacugu agcucucgcc guggacauag 900
auguacuugu cuuauagauc acuguguuau aagcauuggc guggggugcu ucgaugcccu 960
ccuuaguaac cacaauuauc uuucccaguu caggauagac agagccaucu acuccucucg 1020
uaauaagcua uguaguugug gcagaaugug uuacgacuga aucgcgacgc agaucgaugc 1080
ucuuuguuag aaaguccucg gcuucuugac aaacaaucau uuaaggucua caacaagguc 1140
uugccuguca ugcggcuucg acgauuucac cgacgguugc gagguuuucc uuaaaauucu 1200
uggggucgau guuaaguuua caaaguuguu cagggcugag uuggaccugu uuugucaggg 1260
gaaaacguua uaaagccaua ugaagaucug guucccguug auuugucuau gcuuagaaac 1320
cuuaacacau uuggucaaaa caacguucca gcguuugucg aagaucuuuu uaccaauuuu 1380
cuccuuuuua aucuuacaag gcuucuuaau ccucuggauc aguuuguccg ucuaggauga 1440
gaccgaaaua gacacauaga aucucgauua cauggacguu uucaauaggu uucgaaacgg 1500
cuuugaccgg uuaagguuuu cuaucaggag augcgguuuu uucacagaau augagggcug 1560
auacauaagg acgaagcggu ucaguacgcg ugguuagggc ugguuccgcg ucguaaacgu 1620
cccuacaacc aucgccugcu gcuuggaaac cggcuauagu ugguuuagca ccuguaaaag 1680
uaccucguuu uguaccacgu cgucacauga cgcaagaaca accugcggaa uuucuuauug 1740
uccggaugac uccccguaga uguuugaucu gauaaucuuu acuuggacua caggcguggc 1800
guucaucguc ugcgguaaga gccguuguug uacaagugag ugaugcuggc cggggugcau 1860
cgggucgaga cgcuuuuucg accagaaaau guuucucgag aucuugugau gugacuaaac 1920
auacuguagu uugcacgaca ccaugugugg gugaacgaag gucgacuaac caaccaauug 1980
aagaagccuu gagagucgca ccuucuguca aaccucacga acucccggua cgacuggcgc 2040
uuauaagcug ucuuaaacgu cuaaacgcac gucuagcgau gguucauggu gcuuguugag 2100
uggugauucc gaaacuagcu aaacaaacuu ccuaaauucu cgauacuccc aaacaagaua 2160
aaggagccga gguagcaguu gaagaggguu cugguucuuc acgugaaguu cauguagguu 2220
cggcguacgu ucugaccggu uuaguuucuu cagcucgcau agacaucccu uaguuugacg 2280
auguuagggc uuucucaguu cuuaaaggac uuccuucggu uugaaugucu agucaacggc 2340
aacuaauaac agacacuguc uaaacuaaag caggugcuga aucacaacau agauauaucu 2400
uuaagcgacg ucuuuauaua gcucuaaaua caggucuucc aguuaggguc ggcaaauggc 2460
caacaccauc cuccagaaaa ucuacagcug acaaggcuuc uguauuaguu uuuggauuaa 2520
gaacagcagu cuccggucaa gaggugacug cuuaaucauc uucuucaucu uuucuccuug 2580
ucuaacuuug acaacaacgg gaccaaccuc ucgucucaug ugcuuccuac acagcucggu 2640
cgaugcgugu uacgaaaccg guucuagaug uagcuaaggu uguuguuacg gcuuucuaag 2700
aacuuccuuu uaguuaccau gcuaagggcu caacacccug caauaacgcu uuucgcgcua 2760
gguguagacc gaacgcaacg aaugcuugcu ccgguuacgc ugucucuuga cuaucgacau 2820
acauugcuuu ugagagauaa auucagacuu cgagcaauaa accaggcauc ugcacugccu 2880
cuuaauaccc gacuccaaaa cuugcucucg uuaggaaugu cugcagucaa cuaucugguu 2940
caucacguuu gucgaaauuc acuuuggguu cuggggcuuc uauagagaca auggcaauuu 3000
cguaaguacu gucgacuaaa ugguuuacuc gaguaacuua acaaccuuuu cuaacauaac 3060
cuaucaagac acaagaggcu cguaucuuug gaaguuuuaa acgaauagga uugucguuag 3120
uuucggcuac gauguucuca auaccugaug uaguuggcga accuauugau gcuacgggga 3180
cuauagcgcu uauaucggua guuguuggua gagauacuuc uucgaaagcg auaaaaguuc 3240
uuuaaacuac aguuguggag ucgauaaguu cauaacuagc uuguucaguu guuagaccua 3300
gcacgaauac uuaagcgucu ugcaacguua cuuggccggc auaccucagu cgaccgguuu 3360
cgcguugacu ugguuccgaa ucaguuccuu cguuagcuaa gaauguaauu ucgacuacua 3420
ggaagucgaa uauuccugca gcaacucugg cgaagcuuuu uauuaucgac ccuccugaau 3480
cacgccauaa acgucuaccg uucuuucuuc cggucucucu cgauguagcu uagacuuaac 3540
uaaauaagca ugcgcuucug gccuucuaac cgacuaaacc uucuuaagua gucaccuggg 3600
uugguacgac uauaggucuu cuaaccacua uccacgaaac uacuguucua cauacuacga 3660
cgauucgaug agauguuguu acauagguug aagcgagcag agcgauaaug aaaucacgua 3720
gauuuucuua aggucccucg acaacuguca cgauccuucc gauuaucaug gucuuguacc 3780
uuccuucaca caaaacgaac gcagcuacgg cuucuuaagu cuaaccgagu cuacacacca 3840
uacguguagc accaugugcg acuacucaac guucuaaacu aauuaauaau aguucuaucu 3900
ccuaugaaac uucuuaauua gccggauaac cuucgucgua acccgaaccu uucccgugug 3960
uacccauaca aguggcuuaa ucguuaaaac auaagguuua uauucgggcg guuuuacgcg 4020
cuuguagacc uugagaagac cagagcucac uuauaggggu uucaagaauc ccgucgacuu 4080
guucguguaa acacccgucu cgaucacaaa aacauacugu uuauacuucu uauacuauug 4140
cgacacaauc gguacuaccg aguagggugg cuucgaaccg cgcuuccagu aaaguuccua 4200
uaauaauguu uucaacgguu auagcuugaa auaauaucuc gauaaguuaa aaugaaccua 4260
auguuuggcg acaacaauuu acuggaaaac aaccacaacc guggguccua ccuaguauga 4320
ucccgacaaa gaaagaaaug uuuuuguccu gugaauguca accaauuuag gauagaggcc 4380
agacacguuu uaaacuuguu guuucgauag uugcuccggg aguugagaga ugaauagcuu 4440
cuucuucuga aggucccaga cucuugcagg uagcugcgga agcuauugaa gcuguuguag 4500
ccgaaccgag ucuuuaaccu cuucgugcuu gacugccuca agucugcgua gcggcggaug 4560
aacauguuuc cguuguuggc caccuucguc ucgcaucuug acacguuuuu ccuaucugau 4620
aaaucucugc gauaccucau gugaagacuu agaucuuuag uucuuaaccg gcuucucaac 4680
gaccguacca agaaccuauc cuuccggaua cuaacaaaaa gccguacaaa cauagugacg 4740
augcugaaca augccgggcu gcaauaggaa cuugaucgua ccuuuguguu guaauaccug 4800
aagcguuacg gaaaggaaua aguucauuga ucucuugacu gcuguuuuca ucuuuucgau 4860
cuuguuaguc uacggguugc uguuucgcuc cgacgacuuc uuugcuuguu cgguuacuac 4920
uauggucuug ggguugaaua cgauugccga ccgggaccgu acccauaagg ugggguuguc 4980
auacauggag ggguuccgau gcgcgucggu ccgaugcggg gcguuuaccg aauggucccu 5040
auggguccgu acauu 5055
<210> 376
<211> 1684
<212> PRT
<213> 玉米根萤叶甲属
<400> 376
Met Thr Gln Gln Leu Leu Pro Ile Lys Phe Gln Glu His Leu Gln Leu
1 5 10 15
Thr Asn Val Gly Ile His Ala Thr Asn Ile Thr Phe Ala Asn Leu Thr
20 25 30
Met Glu Ser Asp Lys Tyr Ile Cys Val Arg Glu Lys Val Gly Glu Thr
35 40 45
Ser Gln Val Val Ile Ile Asp Met Ala Asp Thr Ala Asn Pro Ile Arg
50 55 60
Arg Pro Ile Thr Ala Glu Ser Ala Ile Met Asn Pro Ala Ser Lys Val
65 70 75 80
Ile Ala Leu Lys Gly Lys Ala Gly Val Glu Thr Gln Lys Thr Leu Gln
85 90 95
Ile Phe Asn Ile Glu Met Lys Ser Lys Met Lys Ala His Thr Met Ser
100 105 110
Glu Asp Val Ile Phe Trp Lys Trp Ile Ser Pro Asn Thr Leu Ala Leu
115 120 125
Val Thr Glu Thr Ser Val Tyr His Trp Ser Met Glu Gly Asp Ser Ile
130 135 140
Pro Gln Lys Met Phe Asp Arg His Ser Ser Leu Asn Gly Cys Gln Ile
145 150 155 160
Ile Asn Tyr Arg Thr Asp Pro Lys Gln Asn Trp Leu Leu Leu Val Gly
165 170 175
Ile Ser Ala Gln Gln Ser Arg Val Val Gly Ala Met Gln Leu Tyr Ser
180 185 190
Val Glu Arg Lys Cys Ser Gln Pro Ile Glu Gly His Ala Ala Ser Phe
195 200 205
Ala Thr Phe Lys Met Glu Gly Asn Pro Glu Pro Ser Thr Leu Phe Cys
210 215 220
Phe Ala Val Arg Thr Leu Gln Gly Gly Lys Leu His Ile Ile Glu Val
225 230 235 240
Gly Gln Ser Pro Ala Gly Asn Gln Ser Phe Pro Lys Lys Thr Val Asp
245 250 255
Val Phe Phe Pro Pro Glu Ala Gln Asn Asp Phe Pro Val Ala Met Gln
260 265 270
Val Ser Ala Lys Tyr Asp Val Ile Tyr Leu Ile Thr Lys Tyr Gly Tyr
275 280 285
Ile His Met Tyr Asp Ile Glu Ser Gly Thr Cys Ile Tyr Met Asn Arg
290 295 300
Ile Ser Ser Asp Thr Ile Phe Val Thr Ala Pro His Glu Ala Thr Gly
305 310 315 320
Gly Ile Ile Gly Val Asn Arg Lys Gly Gln Val Leu Ser Val Ser Val
325 330 335
Asp Glu Glu Ser Ile Ile Arg Tyr Ile Asn Thr Val Leu His Asn Ala
340 345 350
Asp Leu Ala Leu Arg Leu Ala Thr Arg Asn Asn Leu Ser Gly Ala Glu
355 360 365
Glu Leu Phe Val Ser Lys Phe Gln Met Leu Phe Gln Asn Gly Gln Tyr
370 375 380
Ala Glu Ala Ala Lys Val Ala Ala Asn Ala Pro Lys Gly Ile Leu Arg
385 390 395 400
Thr Pro Ala Thr Ile Gln Met Phe Gln Gln Val Pro Thr Gln Pro Gly
405 410 415
Gln Asn Ser Pro Leu Leu Gln Tyr Phe Gly Ile Leu Leu Asp Gln Gly
420 425 430
Gln Leu Asn Arg Tyr Glu Ser Leu Glu Leu Cys Lys Pro Val Leu Leu
435 440 445
Gln Gly Arg Lys Gln Leu Leu Glu Lys Trp Leu Lys Glu Glu Lys Leu
450 455 460
Glu Cys Ser Glu Glu Leu Gly Asp Leu Val Lys Gln Ala Asp Pro Thr
465 470 475 480
Leu Ala Leu Ser Val Tyr Leu Arg Ala Asn Val Pro Ala Lys Val Ile
485 490 495
Gln Ser Phe Ala Glu Thr Gly Gln Phe Gln Lys Ile Val Leu Tyr Ala
500 505 510
Lys Lys Val Ser Tyr Thr Pro Asp Tyr Val Phe Leu Leu Arg Gln Val
515 520 525
Met Arg Thr Asn Pro Asp Gln Gly Ala Ala Phe Ala Gly Met Leu Val
530 535 540
Ala Asp Asp Glu Pro Leu Ala Asp Ile Asn Gln Ile Val Asp Ile Phe
545 550 555 560
Met Glu Gln Asn Met Val Gln Gln Cys Thr Ala Phe Leu Leu Asp Ala
565 570 575
Leu Lys Asn Asn Arg Pro Thr Glu Gly His Leu Gln Thr Arg Leu Leu
580 585 590
Glu Met Asn Leu Met Ser Ala Pro Gln Val Ala Asp Ala Ile Leu Gly
595 600 605
Asn Asn Met Phe Thr His Tyr Asp Arg Pro His Val Ala Gln Leu Cys
610 615 620
Glu Lys Ala Gly Leu Leu Gln Arg Ala Leu Glu His Tyr Thr Asp Leu
625 630 635 640
Tyr Asp Ile Lys Arg Ala Val Val His Thr His Leu Leu Pro Ala Asp
645 650 655
Trp Leu Val Asn Phe Phe Gly Thr Leu Ser Val Glu Asp Ser Leu Glu
660 665 670
Cys Leu Arg Ala Met Leu Thr Ala Asn Ile Arg Gln Asn Leu Gln Ile
675 680 685
Cys Val Gln Ile Ala Thr Lys Tyr His Glu Gln Leu Thr Thr Lys Ala
690 695 700
Leu Ile Asp Leu Phe Glu Gly Phe Lys Ser Tyr Glu Gly Leu Phe Tyr
705 710 715 720
Phe Leu Gly Ser Ile Val Asn Phe Ser Gln Asp Gln Glu Val His Phe
725 730 735
Lys Tyr Ile Gln Ala Ala Cys Lys Thr Gly Gln Ile Lys Glu Val Glu
740 745 750
Arg Ile Cys Arg Glu Ser Asn Cys Tyr Asn Pro Glu Arg Val Lys Asn
755 760 765
Phe Leu Lys Glu Ala Lys Leu Thr Asp Gln Leu Pro Leu Ile Ile Val
770 775 780
Cys Asp Arg Phe Asp Phe Val His Asp Leu Val Leu Tyr Leu Tyr Arg
785 790 795 800
Asn Ser Leu Gln Lys Tyr Ile Glu Ile Tyr Val Gln Lys Val Asn Pro
805 810 815
Ser Arg Leu Pro Val Val Val Gly Gly Leu Leu Asp Val Asp Cys Ser
820 825 830
Glu Asp Ile Ile Lys Asn Leu Ile Leu Val Val Arg Gly Gln Phe Ser
835 840 845
Thr Asp Glu Leu Val Glu Glu Val Glu Lys Arg Asn Arg Leu Lys Leu
850 855 860
Leu Leu Pro Trp Leu Glu Ser Arg Val His Glu Gly Cys Val Glu Pro
865 870 875 880
Ala Thr His Asn Ala Leu Ala Lys Ile Tyr Ile Asp Ser Asn Asn Asn
885 890 895
Ala Glu Arg Phe Leu Lys Glu Asn Gln Trp Tyr Asp Ser Arg Val Val
900 905 910
Gly Arg Tyr Cys Glu Lys Arg Asp Pro His Leu Ala Cys Val Ala Tyr
915 920 925
Glu Arg Gly Gln Cys Asp Arg Glu Leu Ile Ala Val Cys Asn Glu Asn
930 935 940
Ser Leu Phe Lys Ser Glu Ala Arg Tyr Leu Val Arg Arg Arg Asp Gly
945 950 955 960
Glu Leu Trp Ala Glu Val Leu Asn Glu Ser Asn Pro Tyr Arg Arg Gln
965 970 975
Leu Ile Asp Gln Val Val Gln Thr Ala Leu Ser Glu Thr Gln Asp Pro
980 985 990
Glu Asp Ile Ser Val Thr Val Lys Ala Phe Met Thr Ala Asp Leu Pro
995 1000 1005
Asn Glu Leu Ile Glu Leu Leu Glu Lys Ile Val Leu Asp Ser Ser
1010 1015 1020
Val Phe Ser Glu His Arg Asn Leu Gln Asn Leu Leu Ile Leu Thr
1025 1030 1035
Ala Ile Lys Ala Asp Ala Thr Arg Val Met Asp Tyr Ile Asn Arg
1040 1045 1050
Leu Asp Asn Tyr Asp Ala Pro Asp Ile Ala Asn Ile Ala Ile Asn
1055 1060 1065
Asn His Leu Tyr Glu Glu Ala Phe Ala Ile Phe Lys Lys Phe Asp
1070 1075 1080
Val Asn Thr Ser Ala Ile Gln Val Leu Ile Glu Gln Val Asn Asn
1085 1090 1095
Leu Asp Arg Ala Tyr Glu Phe Ala Glu Arg Cys Asn Glu Pro Ala
1100 1105 1110
Val Trp Ser Gln Leu Ala Lys Ala Gln Leu Asn Gln Gly Leu Val
1115 1120 1125
Lys Glu Ala Ile Asp Ser Tyr Ile Lys Ala Asp Asp Pro Ser Ala
1130 1135 1140
Tyr Lys Asp Val Val Glu Thr Ala Ser Lys Asn Asn Ser Trp Glu
1145 1150 1155
Asp Leu Val Arg Tyr Leu Gln Met Ala Arg Lys Lys Ala Arg Glu
1160 1165 1170
Ser Tyr Ile Glu Ser Glu Leu Ile Tyr Ser Tyr Ala Lys Thr Gly
1175 1180 1185
Arg Leu Ala Asp Leu Glu Glu Phe Ile Ser Gly Pro Asn His Ala
1190 1195 1200
Asp Ile Gln Lys Ile Gly Asp Arg Cys Phe Asp Asp Lys Met Tyr
1205 1210 1215
Asp Ala Ala Lys Leu Leu Tyr Asn Asn Val Ser Asn Phe Ala Arg
1220 1225 1230
Leu Ala Ile Thr Leu Val His Leu Lys Glu Phe Gln Gly Ala Val
1235 1240 1245
Asp Ser Ala Arg Lys Ala Asn Ser Thr Arg Thr Trp Lys Glu Val
1250 1255 1260
Cys Phe Ala Cys Val Asp Ala Glu Glu Phe Arg Leu Ala Gln Met
1265 1270 1275
Cys Gly Met His Ile Val Val His Ala Asp Glu Leu Gln Asp Leu
1280 1285 1290
Ile Asn Tyr Tyr Gln Asp Arg Gly Tyr Phe Glu Glu Leu Ile Gly
1295 1300 1305
Leu Leu Glu Ala Ala Leu Gly Leu Glu Arg Ala His Met Gly Met
1310 1315 1320
Phe Thr Glu Leu Ala Ile Leu Tyr Ser Lys Tyr Lys Pro Ala Lys
1325 1330 1335
Met Arg Glu His Leu Glu Leu Phe Trp Ser Arg Val Asn Ile Pro
1340 1345 1350
Lys Val Leu Arg Ala Ala Glu Gln Ala His Leu Trp Ala Glu Leu
1355 1360 1365
Val Phe Leu Tyr Asp Lys Tyr Glu Glu Tyr Asp Asn Ala Val Leu
1370 1375 1380
Ala Met Met Ala His Pro Thr Glu Ala Trp Arg Glu Gly His Phe
1385 1390 1395
Lys Asp Ile Ile Thr Lys Val Ala Asn Ile Glu Leu Tyr Tyr Arg
1400 1405 1410
Ala Ile Gln Phe Tyr Leu Asp Tyr Lys Pro Leu Leu Leu Asn Asp
1415 1420 1425
Leu Leu Leu Val Leu Ala Pro Arg Met Asp His Thr Arg Ala Val
1430 1435 1440
Ser Phe Phe Thr Lys Thr Gly His Leu Gln Leu Val Lys Ser Tyr
1445 1450 1455
Leu Arg Ser Val Gln Asn Leu Asn Asn Lys Ala Ile Asn Glu Ala
1460 1465 1470
Leu Asn Ser Leu Leu Ile Glu Glu Glu Asp Phe Gln Gly Leu Arg
1475 1480 1485
Thr Ser Ile Asp Ala Phe Asp Asn Phe Asp Asn Ile Gly Leu Ala
1490 1495 1500
Gln Lys Leu Glu Lys His Glu Leu Thr Glu Phe Arg Arg Ile Ala
1505 1510 1515
Ala Tyr Leu Tyr Lys Gly Asn Asn Arg Trp Lys Gln Ser Val Glu
1520 1525 1530
Leu Cys Lys Lys Asp Arg Leu Phe Arg Asp Ala Met Glu Tyr Thr
1535 1540 1545
Ser Glu Ser Arg Asn Gln Glu Leu Ala Glu Glu Leu Leu Ala Trp
1550 1555 1560
Phe Leu Asp Arg Lys Ala Tyr Asp Cys Phe Ser Ala Cys Leu Tyr
1565 1570 1575
His Cys Tyr Asp Leu Leu Arg Pro Asp Val Ile Leu Glu Leu Ala
1580 1585 1590
Trp Lys His Asn Ile Met Asp Phe Ala Met Pro Phe Leu Ile Gln
1595 1600 1605
Val Thr Arg Glu Leu Thr Thr Lys Val Glu Lys Leu Glu Gln Ser
1610 1615 1620
Asp Ala Gln Arg Gln Ser Glu Ala Ala Glu Glu Thr Asn Lys Pro
1625 1630 1635
Met Met Ile Pro Glu Pro Gln Leu Met Leu Thr Ala Gly Pro Gly
1640 1645 1650
Met Gly Ile Pro Pro Gln Gln Tyr Val Pro Pro Gln Gly Tyr Ala
1655 1660 1665
Gln Pro Gly Tyr Ala Pro Gln Met Ala Tyr Gln Gly Tyr Pro Gly
1670 1675 1680
Met
<210> 377
<211> 2975
<212> DNA
<213> 巴氏根萤叶甲
<400> 377
atgcaagtgt ccgctaagta cgacgtcatt tacctgatca ccaaatacgg ctatattcat 60
atgtatgaca tcgagagcgg tacctgtatc tacatattcg taaccgcacc ccacgaagct 120
acaggaggaa tcattggtgt taatagaaaa ggtcaagtcc tatccgtctc cgtagacgag 180
gagagcatca ttcgatacat caacaccgtc ttacacaatg ctgacttagc gctgcgtcta 240
gctacgagaa acaatctttc aggagcagaa gaactgtttg ttagtaaatt ccagatgttg 300
ttccagaatg gacagtatgc cgaagctgcc aaagtggctg ccaacgctcc aaaaggaatt 360
ttaagaaccc cagctacaat tcaaatgttt caacaagtcc cgacccaacc tggacaaaac 420
agtcctcttt tgcaatattt cggtatactt ctagaccaag ggcaattgaa cagatatgaa 480
tctttggaac tgtgtaaacc agttttgttg caaggtcgca aacaacttct agaaaaatgg 540
ttaaaagagg aaaaattaga atgttccgaa gaattaggag acctagtcaa acaggcggat 600
cctactctgg ctttatctgt gtatcttaga gctaatgtac ctgcaaaagt tatccaaagc 660
tttgccgaaa caggccaatt ccaaaagata gtcctctacg ccaaaaaagt gtcttatact 720
cccgactatg tattcctgct tcgccaagtc atgcgcacca atcccgacca aggcgccgca 780
ttcgcaggga tgttggtagc agacgacgaa cctttggcag acatcaacca aatcgtggac 840
attttcatgg aacaaaacat ggtgcaacag tgtactgcgt tcctgttgga cgccttaaag 900
aataacaggc ctactgaggg gcatctacaa actagattat tagaaatgaa cctgatgtct 960
gcaccgcaag tggctgacgc catcctcggc aacaacatgt tcactcacta cgaccggccc 1020
cacgtagccc agctctgcga gaaagctggt cttttacaga gagctctaga acactacact 1080
gacttgtatg acatcaaacg tgctgtggta cacactcact tgcttccagc cgattggttg 1140
gttaacttct tcggaactct gagcgtggaa gatagtttgg agtgcttgag ggccatgttg 1200
acggcgaata ttcggcagaa tttacagatt tgtgtgcaga tcgctactaa gtaccacgaa 1260
caactcacca ctaaggcttt gatcgatttg tttgaaggat ttaagagcta tgagggtttg 1320
ttctatttcc tcggctccat cgtcaacttc tcccaagacc aagaagtgca cttcaagtac 1380
atccaagccg catgcaaaac tggccagatc aaagaagtcg agcgtatttg tagggagtcg 1440
aactgctaca atcccgaaag agtcaagaat ttcctgaagg aagccaaact tacagatcag 1500
ttgccgttga ttattgtctg tgacagattt gatttcgtcc acgacttggt gttgtatcta 1560
tatagaaatt cgctgcagaa atatatcgag atttatgtcc agaaggtcaa tcctagccgt 1620
ttaccggttg tagtaggagg tcttttggat gtcgactgtt ccgaagacat aatcaaaaac 1680
cttattctag tcgtcagagg ccagttctcc actgacgaat tagtagaaga agtagaaaag 1740
agaaacagat tgaaactttt gttgccctgg ttggagagca gagtacacga aggatgtgtc 1800
gaaccagcca cgcacaatgc tttggccaag atctacatcg attccaacaa caatgccgaa 1860
agattcttga aggaaaatca atggtacgat tcccgagttg ttggacgtta ttgcgaaaag 1920
cgcgatccac atctggcttg cgttgcttac gaacgaggcc aatgcgacag agaactgata 1980
gctgtatgta acgaaaactc tctattcaag tcagaagcac gttatttggt ccgtagacgt 2040
gacggagaat tatgggctga ggttttgaac gagagcaatc cttacagacg tcagttgata 2100
gaccaagtag tgcaaacagc tttaagtgaa acccaagacc ccgaagatat atccgttacc 2160
gttaaagcat tcatgacagc tgatttacca aatgaactca ttgaattgct ggaaaagatt 2220
gtgctggata gttctgtatt ctccgagcat agaaaccttc aaaacttgct tatcctaaca 2280
gcaatcaaag ccgatgctac aagagttatg gactacatca accgcttgga taactacgat 2340
gctcctgata tcgccaatat agccatcaac aaccatctct atgaagaagc tttcgctatt 2400
ttcaagaaat ttgatgtcaa cacttctgct attcaagtat taatcgaaca agtcaacaac 2460
ctggatcgtg cttacgaatt cgcagaacgt tgcaatgaac cagccgtatg gagccagctg 2520
gccaaagccc aactaaacca aggcttagtt aaagaagcaa tcgattctta cattaaagct 2580
gatgatcctt cagcatacaa ggacgtcgtt gagaccgctt ctaaaaataa tagttgggag 2640
gacttggtcc ggtatttgca gatggcaaga aagaaggcta gagagagcta catcgaatct 2700
gaattgattt attcgtatgc aaagaccgga agattagctg atttggaaga attcatcagt 2760
ggacccaacc atgctgatat ccagaagatt ggtgataggt gctttgacga caagatgtat 2820
gatgctgcca agctactcta caacaatgta tccaacttcg ctcgtctcgc tattacttta 2880
gtgcatctaa aagaattcca gggagctgtt gacagtgcta ggaaggctaa tagtaccaga 2940
acatggaagg aagtgtgttt tgcttgcgtc gatgc 2975
<210> 378
<211> 2975
<212> RNA
<213> 巴氏根萤叶甲
<400> 378
augcaagugu ccgcuaagua cgacgucauu uaccugauca ccaaauacgg cuauauucau 60
auguaugaca ucgagagcgg uaccuguauc uacauauucg uaaccgcacc ccacgaagcu 120
acaggaggaa ucauuggugu uaauagaaaa ggucaagucc uauccgucuc cguagacgag 180
gagagcauca uucgauacau caacaccguc uuacacaaug cugacuuagc gcugcgucua 240
gcuacgagaa acaaucuuuc aggagcagaa gaacuguuug uuaguaaauu ccagauguug 300
uuccagaaug gacaguaugc cgaagcugcc aaaguggcug ccaacgcucc aaaaggaauu 360
uuaagaaccc cagcuacaau ucaaauguuu caacaagucc cgacccaacc uggacaaaac 420
aguccucuuu ugcaauauuu cgguauacuu cuagaccaag ggcaauugaa cagauaugaa 480
ucuuuggaac uguguaaacc aguuuuguug caaggucgca aacaacuucu agaaaaaugg 540
uuaaaagagg aaaaauuaga auguuccgaa gaauuaggag accuagucaa acaggcggau 600
ccuacucugg cuuuaucugu guaucuuaga gcuaauguac cugcaaaagu uauccaaagc 660
uuugccgaaa caggccaauu ccaaaagaua guccucuacg ccaaaaaagu gucuuauacu 720
cccgacuaug uauuccugcu ucgccaaguc augcgcacca aucccgacca aggcgccgca 780
uucgcaggga uguugguagc agacgacgaa ccuuuggcag acaucaacca aaucguggac 840
auuuucaugg aacaaaacau ggugcaacag uguacugcgu uccuguugga cgccuuaaag 900
aauaacaggc cuacugaggg gcaucuacaa acuagauuau uagaaaugaa ccugaugucu 960
gcaccgcaag uggcugacgc cauccucggc aacaacaugu ucacucacua cgaccggccc 1020
cacguagccc agcucugcga gaaagcuggu cuuuuacaga gagcucuaga acacuacacu 1080
gacuuguaug acaucaaacg ugcuguggua cacacucacu ugcuuccagc cgauugguug 1140
guuaacuucu ucggaacucu gagcguggaa gauaguuugg agugcuugag ggccauguug 1200
acggcgaaua uucggcagaa uuuacagauu ugugugcaga ucgcuacuaa guaccacgaa 1260
caacucacca cuaaggcuuu gaucgauuug uuugaaggau uuaagagcua ugaggguuug 1320
uucuauuucc ucggcuccau cgucaacuuc ucccaagacc aagaagugca cuucaaguac 1380
auccaagccg caugcaaaac uggccagauc aaagaagucg agcguauuug uagggagucg 1440
aacugcuaca aucccgaaag agucaagaau uuccugaagg aagccaaacu uacagaucag 1500
uugccguuga uuauugucug ugacagauuu gauuucgucc acgacuuggu guuguaucua 1560
uauagaaauu cgcugcagaa auauaucgag auuuaugucc agaaggucaa uccuagccgu 1620
uuaccgguug uaguaggagg ucuuuuggau gucgacuguu ccgaagacau aaucaaaaac 1680
cuuauucuag ucgucagagg ccaguucucc acugacgaau uaguagaaga aguagaaaag 1740
agaaacagau ugaaacuuuu guugcccugg uuggagagca gaguacacga aggauguguc 1800
gaaccagcca cgcacaaugc uuuggccaag aucuacaucg auuccaacaa caaugccgaa 1860
agauucuuga aggaaaauca augguacgau ucccgaguug uuggacguua uugcgaaaag 1920
cgcgauccac aucuggcuug cguugcuuac gaacgaggcc aaugcgacag agaacugaua 1980
gcuguaugua acgaaaacuc ucuauucaag ucagaagcac guuauuuggu ccguagacgu 2040
gacggagaau uaugggcuga gguuuugaac gagagcaauc cuuacagacg ucaguugaua 2100
gaccaaguag ugcaaacagc uuuaagugaa acccaagacc ccgaagauau auccguuacc 2160
guuaaagcau ucaugacagc ugauuuacca aaugaacuca uugaauugcu ggaaaagauu 2220
gugcuggaua guucuguauu cuccgagcau agaaaccuuc aaaacuugcu uauccuaaca 2280
gcaaucaaag ccgaugcuac aagaguuaug gacuacauca accgcuugga uaacuacgau 2340
gcuccugaua ucgccaauau agccaucaac aaccaucucu augaagaagc uuucgcuauu 2400
uucaagaaau uugaugucaa cacuucugcu auucaaguau uaaucgaaca agucaacaac 2460
cuggaucgug cuuacgaauu cgcagaacgu ugcaaugaac cagccguaug gagccagcug 2520
gccaaagccc aacuaaacca aggcuuaguu aaagaagcaa ucgauucuua cauuaaagcu 2580
gaugauccuu cagcauacaa ggacgucguu gagaccgcuu cuaaaaauaa uaguugggag 2640
gacuuggucc gguauuugca gauggcaaga aagaaggcua gagagagcua caucgaaucu 2700
gaauugauuu auucguaugc aaagaccgga agauuagcug auuuggaaga auucaucagu 2760
ggacccaacc augcugauau ccagaagauu ggugauaggu gcuuugacga caagauguau 2820
gaugcugcca agcuacucua caacaaugua uccaacuucg cucgucucgc uauuacuuua 2880
gugcaucuaa aagaauucca gggagcuguu gacagugcua ggaaggcuaa uaguaccaga 2940
acauggaagg aaguguguuu ugcuugcguc gaugc 2975
<210> 379
<211> 5055
<212> DNA
<213> 黄瓜十一星叶甲
<400> 379
atgacgcagc aactgttacc tatcaagttc caagaacatt tgcagcttac caatgttggt 60
atccatgcta ccaacatcac gttcgccaat ctcaccatgg agagtgacaa gtacatctgc 120
gtgagagaaa aagtcggaga gacgtcccaa gtagtcatca tagatatggc cgacacggcc 180
aatcccatca gacgaccaat caccgccgaa agtgctatta tgaatccagc atccaaagtc 240
attgctctta aaggtaaagc cggcgtagaa acacaaaaaa cacttcaaat atttaacata 300
gaaatgaagt caaaaatgaa agcccacacc atgtccgaag acgtaatttt ctggaaatgg 360
ataagcccca atacgttagc tctagtcacc gagacttcag tgtatcattg gtccatggaa 420
ggtgattcca ctccacagaa aatgttcgac agacactctt ctcttaacgg ctgccaaatc 480
atcaactacc gtacagatcc caaacaaaac tggctgctac ttgtcggtat cagcgctcaa 540
cagtcccgcg tcgttggtgc tatgcaactc tattcagtag aaagaaaatg ctcccagccg 600
atcgaaggac acgccgcttc ttttgccacc ttcaaaatgg aaggaaatcc tgaaccatcg 660
acgctcttct gtttcgcagt gagaacttta cagggaggaa aattgcatat tatcgaggtt 720
ggccaaagtc cagcaggcaa tcaatcgttc cccaaaaaga cagtggacgt tttcttcccg 780
cccgaggccc aaaacgattt tcctgtagcc atgcaagtat cagctaagta cgacgtcatt 840
tacctgatca ccaaatacgg ctacattcat atgtatgata ttgagaccgg cacttgtatc 900
tatatgaaca gaatatccag tgaaaccata ttcgtaaccg caccccacga agctactgga 960
ggaataattg gtgtaaatag aaaaggtcaa gtactatccg tctccgtaga cgaggagagc 1020
atcattcggt acatcaacac cgtcttgcac aatgcagact tagcgctgcg tctagctacg 1080
agaaacaatc tctcaggagc cgaagaactg tttgttagta aatttcagat gttgttccag 1140
aatggacagt acgccgaagc tgccaaagtg gctgccaacg ctccaaaagg aattttaaga 1200
accccagcta caattcaaat gtttcaacaa gtcccgactc aacctggaca aaacagtcct 1260
ctcttacaat atttcggtat acttttagac caaggacaac taaacagata cgaatctttg 1320
gaactatgta aaccagtttt gttgcaaggt cgcaaacagc tgctagaaaa atggttaaaa 1380
gaagaaaaat tagaatgttc cgaggaatta ggagacctag tcaaacaggc agaccctact 1440
ctggctttat ctgtgtatct tagagctaat gtacctgcaa aagttatcca aagctttgct 1500
gaaacaggcc aattccaaaa gatagtcctc tacgccaaaa aagtgtccta tacgcccgac 1560
tatatattcc tgcttcgcca agtcatgcgc accaatcccg accaaggcgc cgcgttcgcc 1620
gggatgttgg tagcagacga agaacctttg gccgacatca accaaatcgt ggacattttc 1680
atggaacaaa acatggtgca gcagtgtact gcgtttttgt tggacgcctt aaagaataac 1740
aggcctactg aggggcatct acaaaccaga ctgttagaaa tgaacctgat gtctgcaccg 1800
caagtggcag acgccattct cggcaacaac atgttcactc actacgaccg gcctcacgta 1860
gcccagctct gcgaaaaagc tggtctttta caaagagctc tggaacacta tactgatttg 1920
tacgatatca aacgtgctgt cgtacacacc cacttgcttc cagctgattg gttggttaac 1980
ttcttcggaa ctctcagcgt ggaagacagt ttggagtgct tgagggccat gctgacggcg 2040
aatattcggc agaatttaca gatttgcgtg cagatcgcta ctaagtacca cgaacaactc 2100
accactaagg ctttgatcga tttgtttgaa gggttcaaga gctatgaggg tttgttctat 2160
ttcctcggct ccatcgtcaa cttctcccaa gaccaagaag tgcacttcaa gtacattcaa 2220
gccgcatgca aaactggcca gatcaaggaa gtggagcgta tttgtaggga atcaaactgc 2280
tacaattccg aaagagtcaa gaatttcctg aaagaagcca aacttacaga tcaattgccg 2340
ttgattattg tctgtgacag atttgatttc gtccacgact tagtgttgta tttatacaga 2400
aactcgctgc agaaatatat cgagatttat gtgcagaaag tcaatcccag ccgtttaccg 2460
gttgtagtag gaggtctttt agatgtcgac tgttccgaag acataatcaa aaacctaatt 2520
ctcgtagtca gaggccaatt ctccactgac gaattagtag aagaagtaga aaagaggaac 2580
agattaaaac tgttgttgcc ctggttagaa agcagagtac acgaaggttg tgtcgaacca 2640
gccacacaca atgctttggc taagatctac atcgattcca acaacaatgc cgaaagattt 2700
ttgaaggaaa atcaatggta cgattcccga gttgtcggac gttattgcga aaagcgcgat 2760
ccacatctgg cttgtgttgc ttacgaacga ggccaatgcg acagagaatt gatagctgta 2820
tgcaacgaaa attctctatt taagtctgaa gcgcgttacc tagtccgtag acgtgacgga 2880
gaattatggg ctgaagtttt gaacgagagc aatcctttca gacgccagtt gatagatcaa 2940
gtagtgcaaa cagctttaag tgaaacccaa gaccctgaag atatttccgt caccgttaaa 3000
gcattcatga cagctgattt accaaatgag ctcattgaat tgctggaaaa gattgtgttg 3060
gataattctg tattctccga gcatagaaac cttcaaaact tgcttatcct tacagcaatc 3120
aaagccgatg ctacaagagt tatggactac atcaaccgct tggacaacta cgatgcccct 3180
gatatcgcga atatagccat caacaaccat ctctacgaag aagctttcgc tattttcaag 3240
aagtttgatg tcaacacctc agctattcaa gtattgatcg aacaagtcaa caatctggat 3300
cgtgcttacg aattcgcaga acgttgcaat gaaccagccg tatggagtca gctggccaaa 3360
gctcaactga accaaggctt agtcaaagaa gcaatcgatt cttacattaa agctgacgat 3420
ccttcggctt acaaggatgt cgtggaaacc gcttcgaaaa ataatagctg ggaggacttg 3480
gtgcggtatt tgcaaatggc aagaaagaag gccagagaga gctacatcga atccgaattg 3540
atttattcgt acgccaagac cggaagattg gctgatttgg aagaattcat cagcgggccc 3600
aaccatgccg atatccagaa gattggtgat aggtgctttg atgacaagat gtatgatgct 3660
gctaagctac tgtacaacaa tgtatccaat ttcgctcgtc tcgctattac tttagttcat 3720
ctaaaagaat tccagggtgc tgttgacagt gctagaaagg ctaacagtac aagaacatgg 3780
aaggaagtgt gttttgcttg tgtcgacgcc gaagaattca gattggctca gatgtgtggt 3840
atgcacatcg tcgtgcacgc tgatgagttg caagacttga ttaattatta ccaggataga 3900
ggatactttg aagaattaat cggcctattg gaagcagcat tgggcttgga aagggcacac 3960
atgggtatgt tcaccgaatt agcaatactg tattccaaat acaagcccgc caaaatgcgc 4020
gaacatctag aactcttctg gtctcgagtg aatatcccca aagttcttag ggcagctgaa 4080
caagcacatt tgtgggctga gctagtgttc ttgtacgaca aatacgaaga atacgataat 4140
gcagtattgg ctatgatggc ccatcctacc gaggcctggc gcgaaggtca tttcaaagat 4200
attattacaa aagtggccaa tatcgaactt tattatagag ctattcaatt ttatctggac 4260
tacaaacccc tgttgttaaa tgaccttttg ttggttttgg cacccagaat ggatcatact 4320
agggctgttt ctttcttcac caaaacagga cacttacagt tggttaaatc ctatctccgt 4380
tctgtgcaaa atttgaacaa caaagctatc aacgaggccc ttaactctct acttattgac 4440
gaagaagact tccagggtct gagaacgtcc atcgatgcct tcgataactt cgacaatatc 4500
ggtttggcgc agaaattaga gaaacacgaa ctgaccgaat ttagacgcat cgccgcctac 4560
ttgtacaaag gaaacaaccg gtggaagcag agcgtagaac tgtgcaaaaa ggatagattg 4620
tttagagacg caatggagta cacttctgaa tctagaaatc aagaattggc cgaggagttg 4680
ctagcctggt tcttggatag gcaggcctat gattgttttt cggcatgttt gtatcactgc 4740
tacgacttgt tacgacctga cgttatcctt gaactagcat ggaaacacaa cattatggac 4800
tttgcgatgc ctttccttat tcaagtaact agagaactga cgacaaaagt agaaaagcta 4860
gaacaatccg acgcccaacg acaaagtgag gctgctgaag aaacgaacaa gccaatgatg 4920
ataccagaac cccaacttat gctaacggct ggccctggca tgggtattcc accccaacag 4980
tatgtacctc cccaagctta cgcgcagcca ggctacgcac cgcaaatggc ttaccaggga 5040
tacccaggca tgtaa 5055
<210> 380
<211> 5055
<212> RNA
<213> 黄瓜十一星叶甲
<400> 380
augacgcagc aacuguuacc uaucaaguuc caagaacauu ugcagcuuac caauguuggu 60
auccaugcua ccaacaucac guucgccaau cucaccaugg agagugacaa guacaucugc 120
gugagagaaa aagucggaga gacgucccaa guagucauca uagauauggc cgacacggcc 180
aaucccauca gacgaccaau caccgccgaa agugcuauua ugaauccagc auccaaaguc 240
auugcucuua aagguaaagc cggcguagaa acacaaaaaa cacuucaaau auuuaacaua 300
gaaaugaagu caaaaaugaa agcccacacc auguccgaag acguaauuuu cuggaaaugg 360
auaagcccca auacguuagc ucuagucacc gagacuucag uguaucauug guccauggaa 420
ggugauucca cuccacagaa aauguucgac agacacucuu cucuuaacgg cugccaaauc 480
aucaacuacc guacagaucc caaacaaaac uggcugcuac uugucgguau cagcgcucaa 540
cagucccgcg ucguuggugc uaugcaacuc uauucaguag aaagaaaaug cucccagccg 600
aucgaaggac acgccgcuuc uuuugccacc uucaaaaugg aaggaaaucc ugaaccaucg 660
acgcucuucu guuucgcagu gagaacuuua cagggaggaa aauugcauau uaucgagguu 720
ggccaaaguc cagcaggcaa ucaaucguuc cccaaaaaga caguggacgu uuucuucccg 780
cccgaggccc aaaacgauuu uccuguagcc augcaaguau cagcuaagua cgacgucauu 840
uaccugauca ccaaauacgg cuacauucau auguaugaua uugagaccgg cacuuguauc 900
uauaugaaca gaauauccag ugaaaccaua uucguaaccg caccccacga agcuacugga 960
ggaauaauug guguaaauag aaaaggucaa guacuauccg ucuccguaga cgaggagagc 1020
aucauucggu acaucaacac cgucuugcac aaugcagacu uagcgcugcg ucuagcuacg 1080
agaaacaauc ucucaggagc cgaagaacug uuuguuagua aauuucagau guuguuccag 1140
aauggacagu acgccgaagc ugccaaagug gcugccaacg cuccaaaagg aauuuuaaga 1200
accccagcua caauucaaau guuucaacaa gucccgacuc aaccuggaca aaacaguccu 1260
cucuuacaau auuucgguau acuuuuagac caaggacaac uaaacagaua cgaaucuuug 1320
gaacuaugua aaccaguuuu guugcaaggu cgcaaacagc ugcuagaaaa augguuaaaa 1380
gaagaaaaau uagaauguuc cgaggaauua ggagaccuag ucaaacaggc agacccuacu 1440
cuggcuuuau cuguguaucu uagagcuaau guaccugcaa aaguuaucca aagcuuugcu 1500
gaaacaggcc aauuccaaaa gauaguccuc uacgccaaaa aaguguccua uacgcccgac 1560
uauauauucc ugcuucgcca agucaugcgc accaaucccg accaaggcgc cgcguucgcc 1620
gggauguugg uagcagacga agaaccuuug gccgacauca accaaaucgu ggacauuuuc 1680
auggaacaaa acauggugca gcaguguacu gcguuuuugu uggacgccuu aaagaauaac 1740
aggccuacug aggggcaucu acaaaccaga cuguuagaaa ugaaccugau gucugcaccg 1800
caaguggcag acgccauucu cggcaacaac auguucacuc acuacgaccg gccucacgua 1860
gcccagcucu gcgaaaaagc uggucuuuua caaagagcuc uggaacacua uacugauuug 1920
uacgauauca aacgugcugu cguacacacc cacuugcuuc cagcugauug guugguuaac 1980
uucuucggaa cucucagcgu ggaagacagu uuggagugcu ugagggccau gcugacggcg 2040
aauauucggc agaauuuaca gauuugcgug cagaucgcua cuaaguacca cgaacaacuc 2100
accacuaagg cuuugaucga uuuguuugaa ggguucaaga gcuaugaggg uuuguucuau 2160
uuccucggcu ccaucgucaa cuucucccaa gaccaagaag ugcacuucaa guacauucaa 2220
gccgcaugca aaacuggcca gaucaaggaa guggagcgua uuuguaggga aucaaacugc 2280
uacaauuccg aaagagucaa gaauuuccug aaagaagcca aacuuacaga ucaauugccg 2340
uugauuauug ucugugacag auuugauuuc guccacgacu uaguguugua uuuauacaga 2400
aacucgcugc agaaauauau cgagauuuau gugcagaaag ucaaucccag ccguuuaccg 2460
guuguaguag gaggucuuuu agaugucgac uguuccgaag acauaaucaa aaaccuaauu 2520
cucguaguca gaggccaauu cuccacugac gaauuaguag aagaaguaga aaagaggaac 2580
agauuaaaac uguuguugcc cugguuagaa agcagaguac acgaagguug ugucgaacca 2640
gccacacaca augcuuuggc uaagaucuac aucgauucca acaacaaugc cgaaagauuu 2700
uugaaggaaa aucaauggua cgauucccga guugucggac guuauugcga aaagcgcgau 2760
ccacaucugg cuuguguugc uuacgaacga ggccaaugcg acagagaauu gauagcugua 2820
ugcaacgaaa auucucuauu uaagucugaa gcgcguuacc uaguccguag acgugacgga 2880
gaauuauggg cugaaguuuu gaacgagagc aauccuuuca gacgccaguu gauagaucaa 2940
guagugcaaa cagcuuuaag ugaaacccaa gacccugaag auauuuccgu caccguuaaa 3000
gcauucauga cagcugauuu accaaaugag cucauugaau ugcuggaaaa gauuguguug 3060
gauaauucug uauucuccga gcauagaaac cuucaaaacu ugcuuauccu uacagcaauc 3120
aaagccgaug cuacaagagu uauggacuac aucaaccgcu uggacaacua cgaugccccu 3180
gauaucgcga auauagccau caacaaccau cucuacgaag aagcuuucgc uauuuucaag 3240
aaguuugaug ucaacaccuc agcuauucaa guauugaucg aacaagucaa caaucuggau 3300
cgugcuuacg aauucgcaga acguugcaau gaaccagccg uauggaguca gcuggccaaa 3360
gcucaacuga accaaggcuu agucaaagaa gcaaucgauu cuuacauuaa agcugacgau 3420
ccuucggcuu acaaggaugu cguggaaacc gcuucgaaaa auaauagcug ggaggacuug 3480
gugcgguauu ugcaaauggc aagaaagaag gccagagaga gcuacaucga auccgaauug 3540
auuuauucgu acgccaagac cggaagauug gcugauuugg aagaauucau cagcgggccc 3600
aaccaugccg auauccagaa gauuggugau aggugcuuug augacaagau guaugaugcu 3660
gcuaagcuac uguacaacaa uguauccaau uucgcucguc ucgcuauuac uuuaguucau 3720
cuaaaagaau uccagggugc uguugacagu gcuagaaagg cuaacaguac aagaacaugg 3780
aaggaagugu guuuugcuug ugucgacgcc gaagaauuca gauuggcuca gauguguggu 3840
augcacaucg ucgugcacgc ugaugaguug caagacuuga uuaauuauua ccaggauaga 3900
ggauacuuug aagaauuaau cggccuauug gaagcagcau ugggcuugga aagggcacac 3960
auggguaugu ucaccgaauu agcaauacug uauuccaaau acaagcccgc caaaaugcgc 4020
gaacaucuag aacucuucug gucucgagug aauaucccca aaguucuuag ggcagcugaa 4080
caagcacauu ugugggcuga gcuaguguuc uuguacgaca aauacgaaga auacgauaau 4140
gcaguauugg cuaugauggc ccauccuacc gaggccuggc gcgaagguca uuucaaagau 4200
auuauuacaa aaguggccaa uaucgaacuu uauuauagag cuauucaauu uuaucuggac 4260
uacaaacccc uguuguuaaa ugaccuuuug uugguuuugg cacccagaau ggaucauacu 4320
agggcuguuu cuuucuucac caaaacagga cacuuacagu ugguuaaauc cuaucuccgu 4380
ucugugcaaa auuugaacaa caaagcuauc aacgaggccc uuaacucucu acuuauugac 4440
gaagaagacu uccagggucu gagaacgucc aucgaugccu ucgauaacuu cgacaauauc 4500
gguuuggcgc agaaauuaga gaaacacgaa cugaccgaau uuagacgcau cgccgccuac 4560
uuguacaaag gaaacaaccg guggaagcag agcguagaac ugugcaaaaa ggauagauug 4620
uuuagagacg caauggagua cacuucugaa ucuagaaauc aagaauuggc cgaggaguug 4680
cuagccuggu ucuuggauag gcaggccuau gauuguuuuu cggcauguuu guaucacugc 4740
uacgacuugu uacgaccuga cguuauccuu gaacuagcau ggaaacacaa cauuauggac 4800
uuugcgaugc cuuuccuuau ucaaguaacu agagaacuga cgacaaaagu agaaaagcua 4860
gaacaauccg acgcccaacg acaaagugag gcugcugaag aaacgaacaa gccaaugaug 4920
auaccagaac cccaacuuau gcuaacggcu ggcccuggca uggguauucc accccaacag 4980
uauguaccuc cccaagcuua cgcgcagcca ggcuacgcac cgcaaauggc uuaccaggga 5040
uacccaggca uguaa 5055
<210> 381
<211> 687
<212> DNA
<213> 玉米根萤叶甲属
<400> 381
atgacgcagc aactgttacc tatcaagttc caagaacatt tgcagcttac caatgttggt 60
atccatgcta ccaacatcac gttcgccaat ctcaccatgg agagcgacaa gtacatctgc 120
gtgagagaaa aagttggaga gacgtcccaa gtagtcatca tagatatggc cgatacggcc 180
aatcccatca gacgaccaat caccgccgaa agtgctatta tgaatccagc atccaaagtc 240
atcgctctta aaggtaaagc aggcgtagaa acacaaaaaa cccttcaaat attcaacata 300
gaaatgaagt caaaaatgaa agcccacacc atgtctgaag acgtaatttt ctggaaatgg 360
atcagcccca atacgttagc gctagtcact gaaacttcag tgtatcattg gtccatggaa 420
ggtgattcaa ttccacagaa aatgtttgac aggcattctt ctcttaacgg ctgccaaatc 480
atcaactacc gaacagatcc caaacaaaac tggctgctac tggtcggtat tagcgctcag 540
caatctcgcg tcgttggtgc tatgcaactg tactcagtag aaagaaaatg ctcgcagcca 600
atcgaaggtc acgccgcttc tttcgcgacc ttcaaaatgg aaggaaaccc tgaaccatcg 660
actctcttct gttttgcagt gaggact 687
<210> 382
<211> 693
<212> DNA
<213> 玉米根萤叶甲属
<400> 382
tcgactctct tctgttttgc agtgaggact ttacaaggag gaaagttaca tattatcgag 60
gttggtcaaa gtccagcagg caaccaatcg tttcctaaaa agacagtgga tgttttcttc 120
ccacccgagg cccaaaatga ttttcctgta gctatgcaag tgtccgctaa atacgacgtc 180
atttacctga ttaccaaata cggctatatt catatgtatg acatcgagag cggcacctgt 240
atctacatga acagaatatc tagtgacaca atattcgtaa ccgcacccca cgaagctacg 300
ggaggaatca ttggtgttaa tagaaagggt caagtcctat ctgtctcggt agatgaggag 360
agcattattc gatacatcaa caccgtctta cacaatgctg acttagcgct gcgtctagct 420
acgagaaaca atctttcagg agccgaagaa ctgtttgtta gtaaattcca gatgttgttc 480
cagaacggac agtacgccga agctgctaaa gtggctgcca acgctccaaa aggaatttta 540
agaaccccag ctacaattca aatgtttcaa caagtcccga ctcaacctgg acaaaacagt 600
ccccttttgc aatatttcgg tatacttcta gaccaagggc aactaaacag atacgaatct 660
ttggaattgt gtaaaccagt tttgttgcaa ggt 693
<210> 383
<211> 700
<212> DNA
<213> 玉米根萤叶甲属
<400> 383
gaattgtgta aaccagtttt gttgcaaggt cgcaaacagc ttctagaaaa atggttaaaa 60
gaggaaaaat tagaatgttc cgaagaatta ggagacctag tcaaacaggc agatcctact 120
ctggctttat ctgtgtatct tagagctaat gtacctgcaa aagttatcca aagctttgcc 180
gaaactggcc aattccaaaa gatagtcctc tacgccaaaa aagtgtctta tactcccgac 240
tatgtattcc tgcttcgcca agtcatgcgc accaatcccg accaaggcgc agcatttgca 300
gggatgttgg tagcggacga cgaacctttg gccgatatca accaaatcgt ggacattttc 360
atggagcaaa acatggtgca gcagtgtact gcgttcttgt tggacgcctt aaagaataac 420
aggcctactg aggggcatct acaaactaga ctattagaaa tgaacctgat gtccgcaccg 480
caagtagcag acgccattct cggcaacaac atgttcactc actacgaccg gccccacgta 540
gcccagctct gcgaaaaagc tggtctttta caaagagctc tagaacacta cactgatttg 600
tatgacatca aacgtgctgt ggtacacacc cacttgcttc cagctgattg gttggttaac 660
ttcttcggaa ctctcagcgt ggaagacagt ttggagtgct 700
<210> 384
<211> 549
<212> DNA
<213> 玉米根萤叶甲属
<400> 384
tcagcgtgga agacagtttg gagtgcttga gggccatgct gaccgcgaat attcgacaga 60
atttgcagat ttgcgtgcag atcgctacca agtaccacga acaactcacc actaaggctt 120
tgatcgattt gtttgaagga tttaagagct atgagggttt gttctatttc ctcggctcca 180
tcgtcaactt ctcccaagac caagaagtgc acttcaagta catccaagcc gcatgcaaga 240
ctggccaaat caaagaagtc gagcgtatct gtagggaatc aaactgctac aatcccgaaa 300
gagtcaagaa tttcctgaag gaagccaaac ttacagatca gttgccgttg attattgtct 360
gtgacagatt tgatttcgtc cacgacttag tgttgtatct atatagaaat tcgctgcaga 420
aatatatcga gatttatgtc cagaaggtca atcccagccg tttaccggtt gtggtaggag 480
gtcttttaga tgtcgactgt tccgaagaca taatcaaaaa cctaattctt gtcgtcagag 540
gccagttct 549
<210> 385
<211> 677
<212> DNA
<213> 玉米根萤叶甲属
<400> 385
taatcaaaaa cctaattctt gtcgtcagag gccagttctc cactgacgaa ttagtagaag 60
aagtagaaaa gaggaacaga ttgaaactgt tgttgccctg gttggagagc agagtacacg 120
aaggatgtgt cgagccagct acgcacaatg ctttggccaa gatctacatc gattccaaca 180
acaatgccga aagattcttg aaggaaaatc aatggtacga ttcccgagtt gtgggacgtt 240
attgcgaaaa gcgcgatcca catctggctt gcgttgctta cgaacgaggc caatgcgaca 300
gagaactgat agctgtatgt aacgaaaact ctctatttaa gtctgaagct cgttatttgg 360
tccgtagacg tgacggagaa ttatgggctg aggttttgaa cgagagcaat ccttacagac 420
gtcagttgat agaccaagta gtgcaaacag ctttaagtga aacccaagac cccgaagata 480
tctctgttac cgttaaagca ttcatgacag ctgatttacc aaatgagctc attgaattgt 540
tggaaaagat tgtattggat agttctgtgt tctccgagca tagaaacctt caaaatttgc 600
ttatcctaac agcaatcaaa gccgatgcta caagagttat ggactacatc aaccgcttgg 660
ataactacga tgcccct 677
<210> 386
<211> 706
<212> DNA
<213> 玉米根萤叶甲属
<400> 386
aaccgcttgg ataactacga tgcccctgat atcgcgaata tagccatcaa caaccatctc 60
tatgaagaag ctttcgctat tttcaagaaa tttgatgtca acacctcagc tattcaagta 120
ttgatcgaac aagtcaacaa tctggatcgt gcttatgaat tcgcagaacg ttgcaatgaa 180
ccggccgtat ggagtcagct ggccaaagcg caactgaacc aaggcttagt caaggaagca 240
atcgattctt acattaaagc tgatgatcct tcagcttata aggacgtcgt tgagaccgct 300
tcgaaaaata atagctggga ggacttagtg cggtatttgc agatggcaag aaagaaggcc 360
agagagagct acatcgaatc tgaattgatt tattcgtacg cgaagaccgg aagattggct 420
gatttggaag aattcatcag tggacccaac catgctgata tccagaagat tggtgatagg 480
tgctttgatg acaagatgta tgatgctgct aagctactct acaacaatgt atccaacttc 540
gctcgtctcg ctattacttt agtgcatcta aaagaattcc agggagctgt tgacagtgct 600
aggaaggcta atagtaccag aacatggaag gaagtgtgtt ttgcttgcgt cgatgccgaa 660
gaattcagat tggctcagat gtgtggtatg cacatcgtgg tacacg 706
<210> 387
<211> 686
<212> DNA
<213> 玉米根萤叶甲属
<400> 387
gcacatcgtg gtacacgctg atgagttgca agatttgatt aattattatc aagatagagg 60
atactttgaa gaattaatcg gcctattgga agcagcattg ggcttggaaa gggcacacat 120
gggtatgttc accgaattag caattttgta ttccaaatat aagcccgcca aaatgcgcga 180
acatctggaa ctcttctggt ctcgagtgaa tatccccaaa gttcttaggg cagctgaaca 240
agcacatttg tgggcagagc tagtgttttt gtatgacaaa tatgaagaat atgataacgc 300
tgtgttagcc atgatggctc atcccaccga agcttggcgc gaaggtcatt tcaaggatat 360
tattacaaaa gttgccaata tcgaacttta ttatagagct attcaatttt acttggatta 420
caaaccgctg ttgttaaatg accttttgtt ggtgttggca cccaggatgg atcatactag 480
ggctgtttct ttctttacaa aaacaggaca cttacagttg gttaaatcct atctccggtc 540
tgtgcaaaat ttgaacaaca aagctatcaa cgaggccctc aactctctac ttatcgaaga 600
agaagacttc cagggtctga gaacgtccat cgacgccttc gataacttcg acaacatcgg 660
cttggctcag aaattggaga agcacg 686
<210> 388
<211> 565
<212> DNA
<213> 玉米根萤叶甲属
<400> 388
cgacaacatc ggcttggctc agaaattgga gaagcacgaa ctgacggagt tcagacgcat 60
cgccgcctac ttgtacaaag gcaacaaccg gtggaagcag agcgtagaac tgtgcaaaaa 120
ggatagacta tttagagacg ctatggagta cacttctgaa tctagaaatc aagaattggc 180
cgaagagttg ctggcatggt tcttggatag gaaggcctat gattgttttt cggcatgttt 240
gtatcactgc tacgacttgt tacggcccga cgttatcctt gaactagcat ggaaacacaa 300
cattatggac ttcgcaatgc ctttccttat tcaagtaact agagaactga cgacaaaagt 360
agaaaagcta gaacaatcag atgcccaacg acaaagcgag gctgctgaag aaacgaacaa 420
gccaatgatg ataccagaac cccaacttat gctaacggct ggccctggca tgggtattcc 480
accccaacag tatgtacctc cccaaggcta cgcgcagcca ggctacgccc cgcaaatggc 540
ttaccaggga tacccaggca tgtaa 565
<210> 389
<211> 687
<212> RNA
<213> 玉米根萤叶甲属
<400> 389
augacgcagc aacuguuacc uaucaaguuc caagaacauu ugcagcuuac caauguuggu 60
auccaugcua ccaacaucac guucgccaau cucaccaugg agagcgacaa guacaucugc 120
gugagagaaa aaguuggaga gacgucccaa guagucauca uagauauggc cgauacggcc 180
aaucccauca gacgaccaau caccgccgaa agugcuauua ugaauccagc auccaaaguc 240
aucgcucuua aagguaaagc aggcguagaa acacaaaaaa cccuucaaau auucaacaua 300
gaaaugaagu caaaaaugaa agcccacacc augucugaag acguaauuuu cuggaaaugg 360
aucagcccca auacguuagc gcuagucacu gaaacuucag uguaucauug guccauggaa 420
ggugauucaa uuccacagaa aauguuugac aggcauucuu cucuuaacgg cugccaaauc 480
aucaacuacc gaacagaucc caaacaaaac uggcugcuac uggucgguau uagcgcucag 540
caaucucgcg ucguuggugc uaugcaacug uacucaguag aaagaaaaug cucgcagcca 600
aucgaagguc acgccgcuuc uuucgcgacc uucaaaaugg aaggaaaccc ugaaccaucg 660
acucucuucu guuuugcagu gaggacu 687
<210> 390
<211> 693
<212> RNA
<213> 玉米根萤叶甲属
<400> 390
ucgacucucu ucuguuuugc agugaggacu uuacaaggag gaaaguuaca uauuaucgag 60
guuggucaaa guccagcagg caaccaaucg uuuccuaaaa agacagugga uguuuucuuc 120
ccacccgagg cccaaaauga uuuuccugua gcuaugcaag uguccgcuaa auacgacguc 180
auuuaccuga uuaccaaaua cggcuauauu cauauguaug acaucgagag cggcaccugu 240
aucuacauga acagaauauc uagugacaca auauucguaa ccgcacccca cgaagcuacg 300
ggaggaauca uugguguuaa uagaaagggu caaguccuau cugucucggu agaugaggag 360
agcauuauuc gauacaucaa caccgucuua cacaaugcug acuuagcgcu gcgucuagcu 420
acgagaaaca aucuuucagg agccgaagaa cuguuuguua guaaauucca gauguuguuc 480
cagaacggac aguacgccga agcugcuaaa guggcugcca acgcuccaaa aggaauuuua 540
agaaccccag cuacaauuca aauguuucaa caagucccga cucaaccugg acaaaacagu 600
ccccuuuugc aauauuucgg uauacuucua gaccaagggc aacuaaacag auacgaaucu 660
uuggaauugu guaaaccagu uuuguugcaa ggu 693
<210> 391
<211> 700
<212> RNA
<213> 玉米根萤叶甲属
<400> 391
gaauugugua aaccaguuuu guugcaaggu cgcaaacagc uucuagaaaa augguuaaaa 60
gaggaaaaau uagaauguuc cgaagaauua ggagaccuag ucaaacaggc agauccuacu 120
cuggcuuuau cuguguaucu uagagcuaau guaccugcaa aaguuaucca aagcuuugcc 180
gaaacuggcc aauuccaaaa gauaguccuc uacgccaaaa aagugucuua uacucccgac 240
uauguauucc ugcuucgcca agucaugcgc accaaucccg accaaggcgc agcauuugca 300
gggauguugg uagcggacga cgaaccuuug gccgauauca accaaaucgu ggacauuuuc 360
auggagcaaa acauggugca gcaguguacu gcguucuugu uggacgccuu aaagaauaac 420
aggccuacug aggggcaucu acaaacuaga cuauuagaaa ugaaccugau guccgcaccg 480
caaguagcag acgccauucu cggcaacaac auguucacuc acuacgaccg gccccacgua 540
gcccagcucu gcgaaaaagc uggucuuuua caaagagcuc uagaacacua cacugauuug 600
uaugacauca aacgugcugu gguacacacc cacuugcuuc cagcugauug guugguuaac 660
uucuucggaa cucucagcgu ggaagacagu uuggagugcu 700
<210> 392
<211> 549
<212> RNA
<213> 玉米根萤叶甲属
<400> 392
ucagcgugga agacaguuug gagugcuuga gggccaugcu gaccgcgaau auucgacaga 60
auuugcagau uugcgugcag aucgcuacca aguaccacga acaacucacc acuaaggcuu 120
ugaucgauuu guuugaagga uuuaagagcu augaggguuu guucuauuuc cucggcucca 180
ucgucaacuu cucccaagac caagaagugc acuucaagua cauccaagcc gcaugcaaga 240
cuggccaaau caaagaaguc gagcguaucu guagggaauc aaacugcuac aaucccgaaa 300
gagucaagaa uuuccugaag gaagccaaac uuacagauca guugccguug auuauugucu 360
gugacagauu ugauuucguc cacgacuuag uguuguaucu auauagaaau ucgcugcaga 420
aauauaucga gauuuauguc cagaagguca aucccagccg uuuaccgguu gugguaggag 480
gucuuuuaga ugucgacugu uccgaagaca uaaucaaaaa ccuaauucuu gucgucagag 540
gccaguucu 549
<210> 393
<211> 677
<212> RNA
<213> 玉米根萤叶甲属
<400> 393
uaaucaaaaa ccuaauucuu gucgucagag gccaguucuc cacugacgaa uuaguagaag 60
aaguagaaaa gaggaacaga uugaaacugu uguugcccug guuggagagc agaguacacg 120
aaggaugugu cgagccagcu acgcacaaug cuuuggccaa gaucuacauc gauuccaaca 180
acaaugccga aagauucuug aaggaaaauc aaugguacga uucccgaguu gugggacguu 240
auugcgaaaa gcgcgaucca caucuggcuu gcguugcuua cgaacgaggc caaugcgaca 300
gagaacugau agcuguaugu aacgaaaacu cucuauuuaa gucugaagcu cguuauuugg 360
uccguagacg ugacggagaa uuaugggcug agguuuugaa cgagagcaau ccuuacagac 420
gucaguugau agaccaagua gugcaaacag cuuuaaguga aacccaagac cccgaagaua 480
ucucuguuac cguuaaagca uucaugacag cugauuuacc aaaugagcuc auugaauugu 540
uggaaaagau uguauuggau aguucugugu ucuccgagca uagaaaccuu caaaauuugc 600
uuauccuaac agcaaucaaa gccgaugcua caagaguuau ggacuacauc aaccgcuugg 660
auaacuacga ugccccu 677
<210> 394
<211> 706
<212> RNA
<213> 玉米根萤叶甲属
<400> 394
aaccgcuugg auaacuacga ugccccugau aucgcgaaua uagccaucaa caaccaucuc 60
uaugaagaag cuuucgcuau uuucaagaaa uuugauguca acaccucagc uauucaagua 120
uugaucgaac aagucaacaa ucuggaucgu gcuuaugaau ucgcagaacg uugcaaugaa 180
ccggccguau ggagucagcu ggccaaagcg caacugaacc aaggcuuagu caaggaagca 240
aucgauucuu acauuaaagc ugaugauccu ucagcuuaua aggacgucgu ugagaccgcu 300
ucgaaaaaua auagcuggga ggacuuagug cgguauuugc agauggcaag aaagaaggcc 360
agagagagcu acaucgaauc ugaauugauu uauucguacg cgaagaccgg aagauuggcu 420
gauuuggaag aauucaucag uggacccaac caugcugaua uccagaagau uggugauagg 480
ugcuuugaug acaagaugua ugaugcugcu aagcuacucu acaacaaugu auccaacuuc 540
gcucgucucg cuauuacuuu agugcaucua aaagaauucc agggagcugu ugacagugcu 600
aggaaggcua auaguaccag aacauggaag gaaguguguu uugcuugcgu cgaugccgaa 660
gaauucagau uggcucagau gugugguaug cacaucgugg uacacg 706
<210> 395
<211> 686
<212> RNA
<213> 玉米根萤叶甲属
<400> 395
gcacaucgug guacacgcug augaguugca agauuugauu aauuauuauc aagauagagg 60
auacuuugaa gaauuaaucg gccuauugga agcagcauug ggcuuggaaa gggcacacau 120
ggguauguuc accgaauuag caauuuugua uuccaaauau aagcccgcca aaaugcgcga 180
acaucuggaa cucuucuggu cucgagugaa uauccccaaa guucuuaggg cagcugaaca 240
agcacauuug ugggcagagc uaguguuuuu guaugacaaa uaugaagaau augauaacgc 300
uguguuagcc augauggcuc aucccaccga agcuuggcgc gaaggucauu ucaaggauau 360
uauuacaaaa guugccaaua ucgaacuuua uuauagagcu auucaauuuu acuuggauua 420
caaaccgcug uuguuaaaug accuuuuguu gguguuggca cccaggaugg aucauacuag 480
ggcuguuucu uucuuuacaa aaacaggaca cuuacaguug guuaaauccu aucuccgguc 540
ugugcaaaau uugaacaaca aagcuaucaa cgaggcccuc aacucucuac uuaucgaaga 600
agaagacuuc cagggucuga gaacguccau cgacgccuuc gauaacuucg acaacaucgg 660
cuuggcucag aaauuggaga agcacg 686
<210> 396
<211> 565
<212> RNA
<213> 玉米根萤叶甲属
<400> 396
cgacaacauc ggcuuggcuc agaaauugga gaagcacgaa cugacggagu ucagacgcau 60
cgccgccuac uuguacaaag gcaacaaccg guggaagcag agcguagaac ugugcaaaaa 120
ggauagacua uuuagagacg cuauggagua cacuucugaa ucuagaaauc aagaauuggc 180
cgaagaguug cuggcauggu ucuuggauag gaaggccuau gauuguuuuu cggcauguuu 240
guaucacugc uacgacuugu uacggcccga cguuauccuu gaacuagcau ggaaacacaa 300
cauuauggac uucgcaaugc cuuuccuuau ucaaguaacu agagaacuga cgacaaaagu 360
agaaaagcua gaacaaucag augcccaacg acaaagcgag gcugcugaag aaacgaacaa 420
gccaaugaug auaccagaac cccaacuuau gcuaacggcu ggcccuggca uggguauucc 480
accccaacag uauguaccuc cccaaggcua cgcgcagcca ggcuacgccc cgcaaauggc 540
uuaccaggga uacccaggca uguaa 565
<210> 397
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 397
aagaaaaagc aacatgacgc 20
<210> 398
<211> 20
<212> DNA
<213> 玉米根萤叶甲属
<400> 398
ggggccttat attacatgcc 20
<210> 399
<211> 130
<212> DNA
<213> 玉米根萤叶甲属
<400> 399
gatatcacag acgtattgta gacctagaaa ataagaaatt tgacctcgaa aaagaagtgg 60
aattcagaga ttttcagatc tccgaattga acagccaagt aaacgacctt agaggcaaat 120
tcgtcaaacc 130
<210> 400
<211> 119
<212> DNA
<213> 玉米根萤叶甲属
<400> 400
gtaaccaact caaagttgtc aagaagaaag aattcacctt agaagaagaa gacaaagaaa 60
agaaaccaga ctggtcaaag aagggagacg aaaagaaggt acaagaggct gaagcatga 119
<210> 401
<211> 130
<212> RNA
<213> 玉米根萤叶甲属
<400> 401
gauaucacag acguauugua gaccuagaaa auaagaaauu ugaccucgaa aaagaagugg 60
aauucagaga uuuucagauc uccgaauuga acagccaagu aaacgaccuu agaggcaaau 120
ucgucaaacc 130
<210> 402
<211> 119
<212> RNA
<213> 玉米根萤叶甲属
<400> 402
guaaccaacu caaaguuguc aagaagaaag aauucaccuu agaagaagaa gacaaagaaa 60
agaaaccaga cuggucaaag aagggagacg aaaagaaggu acaagaggcu gaagcauga 119
<210> 403
<211> 692
<212> DNA
<213> 人工序列
<220>
<223> 玉米根萤叶甲属、 拟南芥
<400> 403
gaccttagag gcaaattcgt caaaccaacc ttgaagaagg tatccaaata cgaaaacaaa 60
ttcgccaaac ttcaaaagaa ggcagctgaa tttaacttcc gtaaccaact caaagttgtc 120
aagaagaaag aatggtacca agctgcgaat cttcgttttt ttaaggaatt ctcgatcttt 180
atggtgtata ggctctgggt tttctgtttt ttgtatctct taggattttg taaattccag 240
atctttctat ggccacttag tagtatattt caaaaattct ccaatcgagt tcttcattcg 300
cattttcagt cattttctct tcgacgttgt ttttaagcct gggtattact cctatttagt 360
tgaactctgc agcaatctta gaaaattagg gttttgaggt ttcgatttct ctaggtaacc 420
gatctattgc attcatctga atttctgcat atatgtctta gatttctgat aagcttacga 480
tacgttaggt gtaattgaag tttatttttc aagagtgtta ttttttgttt ctgaattttt 540
cagtcactcc atggcctaga ttctttcttc ttgacaactt tgagttggtt acggaagtta 600
aattcagctg ccttcttttg aagtttggcg aatttgtttt cgtatttgga taccttcttc 660
aaggttggtt tgacgaattt gcctctaagg tc 692

Claims (70)

1.一种干扰核糖核酸(RNA)分子，其中该RNA包含至少一种dsRNA，其中该dsRNA是包含退火的互补链的双链RNA的区域，其中的一条链包含具有至少19个连续核苷酸的序列，该序列与在根萤叶甲属(Diabrotica spp)靶基因中的靶核苷酸序列是至少部分互补的，并且(i)至少与SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19个连续核苷酸的片段，或其互补序列具有至少85％同一性；(ii)至少包含SEQ ID NO:121-210，SEQ IDNO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19个连续核苷酸的片段，或其互补序列；(iii)至少包含编码由SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400编码的氨基酸序列的核苷酸序列的19个连续核苷酸的片段，或其互补序列，或者(iv)可以在严格条件下与选自下组的多核苷酸杂交，该组由以下组成：SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400，及其互补序列，其中该干扰RNA分子对鞘翅目植物有害生物具有杀昆虫活性。
2.如权利要求1所述的干扰RNA分子，其中所述鞘翅目植物有害生物是根萤叶甲属昆虫。
3.如权利要求1所述的干扰RNA分子，其中该RNA包含至少两种dsRNA，其中每种dsRNA包含核苷酸的序列，该序列与该靶基因中的靶核苷酸序列是至少部分互补的。
4.如权利要求3所述的干扰RNA分子，其中所述dsRNA中的每种包含不同的核苷酸序列，该序列与该靶基因中的不同靶核苷酸序列是至少部分互补的。
5.如权利要求1所述的干扰RNA分子，其中该干扰RNA分子包含SEQ ID NO:121-210，SEQID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ IDNO:373、374、378、380、389-396、399、400，或其互补序列。
6.如权利要求1所述的干扰RNA分子，其中该dsRNA是含有基本上互补的退火链的双链RNA的区域。
7.如权利要求1所述的干扰RNA分子，其中该dsRNA是含有完全互补的退火链的双链RNA的区域。
8.如权利要求1至7中任一项所述的干扰RNA分子，其中该根萤叶甲属昆虫选自下组，该组由以下组成：巴氏根萤叶甲(D.barberi)、西方玉米根萤叶甲(D.virgifera)、黄瓜十一星叶甲(D.undecimpunctata)、黄瓜条根萤叶甲(D.balteata)、黄瓜十一星叶甲(D.undecimpunctata)、斯格尼根萤叶甲(D.significata)以及南美叶甲(D.speciosa)。
9.一种核酸构建体，其包含如权利要求1至8中任一项所述的干扰RNA分子。
10.一种核酸分子，其编码如权利要求1至8中任一项所述的干扰RNA分子。
11.一种核酸构建体，其包含编码如权利要求10所述的核酸分子的核苷酸序列。
12.如权利要求9或11中任一项所述的核酸构建体，其中该核酸构建体是表达载体。
13.一种重组载体，其包含可操作地连接至如下核苷酸序列的调节序列，该核苷酸序列编码如权利要求1至8中任一项所述的干扰RNA分子。
14.一种组合物，其包含如权利要求1至8中任一项所述的干扰RNA分子中的两种或更多种。
15.如权利要求14所述的组合物，其中该两种或更多种干扰RNA分子存在于相同的核酸构建体上、不同的核酸构建体上或其任何组合上。
16.如权利要求14或15中任一项所述的组合物，该组合物包含含有至少一种dsRNA的干扰RNA分子，其中该dsRNA是包含退火的互补链的双链RNA的区域，其中的一条链包含具有至少19个连续核苷酸的序列，该序列(i)至少与SEQ ID NO:121-210，SEQ ID NO:274-276，SEQID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19个连续核苷酸的片段，或其互补序列具有至少85％同一性；或者(ii)至少包含SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19个连续核苷酸的片段，或其互补序列。
17.一种组合物，其包含如权利要求9、11或12中任一项所述的核酸构建体中的两种或更多种，其中该两种或更多种核酸构建体各自包含不同的干扰RNA。
18.一种组合物，其包含如权利要求10所述的核酸分子中的两种或更多种，其中该两种或更多种核酸分子各自编码不同的干扰RNA分子。
19.一种用于抑制根萤叶甲属昆虫靶基因表达的杀昆虫组合物，该组合物包含如权利要求1至8中任一项所述的干扰RNA，以及农业上可接受的载体。
20.如权利要求19所述的杀昆虫组合物，其至少包含用于控制根萤叶甲属昆虫的第二杀昆虫剂。
21.如权利要求20所述的杀昆虫组合物，其中该第二杀昆虫剂是苏云金芽孢杆菌(Bacillus thuringiensis)杀昆虫蛋白。
22.如权利要求20所述的杀昆虫组合物，其中该第二杀昆虫剂不是苏云金芽孢杆菌杀昆虫蛋白。
23.如权利要求20所述的杀昆虫组合物，其中该第二杀昆虫剂是马铃薯糖蛋白、蛋白酶、蛋白酶抑制剂、脲酶、α-淀粉酶抑制剂、成孔蛋白、凝集素、工程化抗体或抗体片段、或几丁质酶。
24.如权利要求22所述的杀昆虫组合物，其中该第二杀昆虫剂是或源自蜡样芽孢杆菌(Bacillus cereus)杀昆虫蛋白、致病杆菌属(Xenorhabdus spp.)杀昆虫蛋白、光杆菌属(Photorhabdus spp.)杀昆虫蛋白、侧孢短芽孢杆菌(Brevibacillus laterosporous)杀昆虫蛋白、球形赖氨酸芽胞杆菌(Lysinibacillus sphearicus)杀昆虫蛋白、色杆菌属(Chromobacterium spp.)杀昆虫蛋白、噬虫霉耶尔森菌(Yersinia entomophaga)杀昆虫蛋白、乳状芽孢杆菌(Paenibacillus popiliae)杀昆虫蛋白、或梭菌属(Clostridium spp.)杀昆虫蛋白。
25.一种转基因植物或其部分，包含如对应的前述权利要求中任一项所述的干扰RNA分子、核酸分子、核酸构建体和/或组合物，其中当与对照植物相比时，该转基因植物对根萤叶甲属昆虫具有增强的抗性。
26.如权利要求25所述的转基因植物或其部分，其中该转基因植物至少包含用于控制根萤叶甲属昆虫的第二杀昆虫剂。
27.如权利要求26所述的转基因植物或其部分，其中该第二杀昆虫剂是苏云金芽孢杆菌杀昆虫蛋白。
28.如权利要求26所述的转基因植物或其部分，其中该第二杀昆虫剂不是苏云金芽孢杆菌杀昆虫蛋白。
29.如权利要求26所述的转基因植物或其部分，其中该第二杀昆虫剂是马铃薯糖蛋白、蛋白酶、蛋白酶抑制剂、脲酶、α-淀粉酶抑制剂、成孔蛋白、凝集素、工程化抗体或抗体片段、或几丁质酶。
30.如权利要求28所述的转基因植物或其部分，其中该第二杀昆虫剂是或源自蜡样芽孢杆菌杀昆虫蛋白、致病杆菌属杀昆虫蛋白、光杆菌属杀昆虫蛋白、侧孢短芽孢杆菌杀昆虫蛋白、球形赖氨酸芽胞杆菌杀昆虫蛋白、色杆菌属杀昆虫蛋白、噬虫霉耶尔森菌杀昆虫蛋白、乳状芽孢杆菌杀昆虫蛋白、或梭菌属杀昆虫蛋白。
31.如权利要求25至30中任一项所述的转基因植物或其部分，其中该转基因植物或其部分是玉蜀黍植物或其部分。
32.一种转基因种子，其是如权利要求25至31中任一项所述的转基因植物的转基因种子。
33.一种生物样品，其来自如权利要求25至31中任一项所述的转基因植物或其部分。
34.一种商品产品，其源自如权利要求25至31中任一项所述的转基因植物或其部分。
35.如权利要求34所述的商品产品，其中该商品产品选自下组，该组由以下各项组成：整个或经处理的种子、豆类、谷物、籽粒、壳、粉、粗碾去壳谷类、面粉、糖、糖、淀粉、蛋白质浓缩物、蛋白质分离物、蜡、油、提取物、汁、浓缩物、液体、糖浆、饲料、青贮饲料、纤维、纸或者其他从植物生产的食物或产品。
36.一种控制根萤叶甲属昆虫的方法，该方法包括使该根萤叶甲属昆虫与作为如权利要求1-8所述的干扰RNA分子或能够产生如权利要求1-8所述的干扰RNA分子的核酸分子接触，该干扰RNA分子用于抑制靶基因在根萤叶甲属昆虫中的表达，从而控制该根萤叶甲属昆虫。
37.如权利要求36所述的方法，其中该靶基因包含如下编码序列，该编码序列：
a)至少与SEQ ID NO:1-30，SEQ ID NO:91-120，SEQ ID NO:271-273，SEQ ID NO:277-279，SEQ ID NO:283-297，SEQ ID NO:313-317，SEQ ID NO:372、377、379，SEQ ID NO:381-388，SEQ ID NO:399；或SEQ ID NO:400的19个核苷酸的连续片段具有至少85％同一性；
b)至少包含SEQ ID NO:1-30，SEQ ID NO:91-120，SEQ ID NO:271-273，SEQ ID NO:277-279，SEQ ID NO:283-297，SEQ ID NO:313-317，SEQ ID NO:372、377、379，SEQ ID NO:381-388，SEQ ID NO:399；或SEQ ID NO:400的19个核苷酸的连续片段；或者
c)至少包含编码由SEQ ID NO:1-30，SEQ ID NO:91-120，SEQ ID NO:271-273，SEQ IDNO:277-279，SEQ ID NO:283-297，SEQ ID NO:313-317，SEQ ID NO:372、377、379，SEQ IDNO:381-388，SEQ ID NO:399；或SEQ ID NO:400编码的氨基酸序列的核苷酸序列的19个核苷酸的连续片段。
38.如权利要求36所述的方法，其中该干扰RNA分子包含至少一种dsRNA，其中该dsRNA是包含退火的互补链的双链RNA的区域，其中的一条链包含具有至少19个连续核苷酸的序列，该序列(i)至少与SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19个连续核苷酸的片段，或其互补序列具有至少85％同一性；或者(ii)至少包含SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400的19个连续核苷酸的片段，或其互补序列；或者(iii)至少包含编码由SEQ ID NO:121-210，SEQ ID NO:274-276，SEQ ID NO:280-282，SEQ ID NO:298-312，SEQ ID NO:345-371，SEQ ID NO:373、374、378、380、389-396、399、400编码的氨基酸序列的核苷酸序列的19个连续核苷酸的片段，或其互补序列。
39.如权利要求36至38中任一项所述的方法，其中该根萤叶甲属昆虫选自下组，该组由以下组成：巴氏根萤叶甲、西方玉米根萤叶甲、黄瓜十一星叶甲、黄瓜条根萤叶甲、黄瓜十一星叶甲、斯格尼根萤叶甲以及南美叶甲。
40.如权利要求39所述的方法，其中接触包括：
a)种植转基因种子，该转基因种子能够产生一种表达该核酸分子的转基因植物，其中该根萤叶甲属昆虫摄食该转基因植物或其部分；或者
b)将包含该核酸分子的组合物施用至种子或植物或其部分，其中该根萤叶甲属昆虫摄食该种子、该植物或其部分。
41.如权利要求39所述的方法，其中该转基因种子和转基因植物是玉米种子和玉米植物。
42.如权利要求39所述的方法，其中该种子或植物是玉米种子或玉米植物。
43.一种控制根萤叶甲属昆虫的方法，该方法包括使该根萤叶甲属昆虫与作为如权利要求1-8所述的干扰RNA分子或能够产生如权利要求1-8所述的干扰RNA分子的核酸分子接触，该干扰RNA分子用于抑制靶基因在根萤叶甲属昆虫中的表达，并且使该根萤叶甲属昆虫至少与第二杀昆虫剂接触以控制根萤叶甲属。
44.一种控制根萤叶甲属昆虫的方法，该方法包括使该根萤叶甲属昆虫与作为如权利要求1-8所述的干扰RNA分子或能够产生如权利要求1-8所述的干扰RNA分子的核酸分子接触，该干扰RNA分子用于抑制靶基因在根萤叶甲属昆虫中的表达，并且使该根萤叶甲属昆虫至少与第二杀昆虫剂接触以控制根萤叶甲属，从而控制该根萤叶甲属昆虫，其中所述第二杀昆虫剂包含苏云金芽孢杆菌杀昆虫蛋白。
45.一种控制根萤叶甲属昆虫的方法，该方法包括使该根萤叶甲属昆虫与作为如权利要求1-8所述的干扰RNA分子或能够产生如权利要求1-8所述的干扰RNA分子的核酸分子接触，该干扰RNA分子用于抑制靶基因在根萤叶甲属昆虫中的表达，并且使该根萤叶甲属昆虫至少与第二杀昆虫剂接触以控制根萤叶甲属，从而控制该根萤叶甲属昆虫，其中所述第二杀昆虫剂不包含苏云金芽孢杆菌杀昆虫蛋白。
46.如权利要求43所述的方法，其中该第二杀昆虫剂包含马铃薯糖蛋白、蛋白酶、蛋白酶抑制剂、脲酶、α-淀粉酶抑制剂、成孔蛋白、凝集素、工程化抗体或抗体片段、或几丁质酶。
47.如权利要求45所述的方法，其中该第二杀昆虫剂包含蜡样芽孢杆菌杀昆虫蛋白、致病杆菌属杀昆虫蛋白、光杆菌属杀昆虫蛋白、侧孢短芽孢杆菌杀昆虫蛋白、球形赖氨酸芽胞杆菌杀昆虫蛋白、色杆菌属杀昆虫蛋白、噬虫霉耶尔森菌杀昆虫蛋白、乳状芽孢杆菌杀昆虫蛋白、或梭菌属杀昆虫蛋白。
48.一种减少转基因植物上的成虫根萤叶甲属昆虫种群的方法，该转基因植物表达Cry蛋白、杂种Cry蛋白或修饰的Cry蛋白，该方法包括在该转基因植物中表达作为如权利要求1所述的干扰RNA分子或能够产生如权利要求1所述的干扰RNA分子的核酸分子，该干扰RNA分子抑制靶基因在成虫根萤叶甲属昆虫中的表达，从而减少该成虫根萤叶甲属昆虫种群。
49.一种减少对如权利要求1所述的干扰RNA分子的抗性在根萤叶甲属昆虫种群中的发展的方法，该方法包括在被该根萤叶甲属昆虫种群摄食的转基因植物中表达如权利要求1所述的干扰RNA分子，该干扰RNA分子抑制靶基因在幼虫和成虫根萤叶甲属昆虫中的表达，从而与暴露于能够抑制靶基因在幼虫或成虫根萤叶甲属昆虫中的表达的干扰RNA分子的根萤叶甲属昆虫种群相比，减少抗性在该根萤叶甲属昆虫种群中的发展。
50.一种降低从靶基因转录的靶RNA在根萤叶甲属昆虫中的水平的方法，该方法包括使该根萤叶甲属昆虫与包含如权利要求1至8中任一项所述的干扰RNA分子的组合物接触，其中该干扰RNA分子降低该靶RNA在该根萤叶甲属昆虫的细胞中的水平。
51.如权利要求50所述的方法，其中该靶RNA编码的蛋白质的产生减少。
52.如权利要求51所述的方法，其中该蛋白质包含与SEQ ID NO:241-270或SEQ ID NO:376具有至少85％同一性的氨基酸序列。
53.如权利要求50所述的方法，其中该干扰RNA来自表达该干扰RNA的转基因生物体。
54.如权利要求53所述的方法，其中该转基因生物体是转基因植物、转基因微生物、转基因细菌或转基因内寄生菌。
55.如权利要求50所述的方法，其中该干扰RNA对于根萤叶甲属昆虫是致死的。
56.如权利要求55所述的方法，其中该根萤叶甲属昆虫选自下组，该组由以下组成：巴氏根萤叶甲、西方玉米根萤叶甲(D.virgifera virgifera)、黄瓜十一星叶甲、黄瓜条根萤叶甲、黄瓜十一星叶甲、斯格尼根萤叶甲以及南美叶甲。
57.一种赋予植物或其部分对根萤叶甲属昆虫耐受性的方法，该方法包括向该植物或其部分中引入如对应的前述权利要求中任一项所述的干扰RNA分子、核酸分子、核酸构建体、和/或组合物，从而赋予该植物或其部分对该根萤叶甲属昆虫的耐受性。
58.一种减少被根萤叶甲属昆虫摄食的植物的根损害的方法，该方法包括向该植物的细胞中引入如对应的前述权利要求中任一项所述的干扰RNA分子、核酸分子、核酸构建体、和/或组合物，从而减少对该植物的根损害。
59.一种产生对根萤叶甲属昆虫具有毒性的转基因植物细胞的方法，该方法包括向植物细胞中引入如对应的前述权利要求中任一项所述的干扰RNA、核酸分子、核酸构建体、和/或组合物，从而产生与对照植物细胞相比，对该根萤叶甲属昆虫具有毒性的转基因植物细胞。
60.多个转基因植物细胞，其由如权利要求59所述的方法产生。
61.如权利要求60所述的多个转基因植物细胞，其中使这些植物细胞在包括天然日光的条件下生长。
62.一种产生对根萤叶甲属昆虫摄食损害具有增强的耐受性的转基因植物的方法，该方法包括向植物中引入如对应的前述权利要求中任一项所述的干扰RNA分子、核酸分子、或核酸构建体，从而产生与对照植物相比，对根萤叶甲属昆虫摄食损害具有增强的耐受性的转基因植物。
63.如权利要求62所述的方法，其中通过转化植物细胞并且自该转化的植物细胞产生该转基因植物来进行该引入步骤。
64.如权利要求62所述的方法，其中通过共同培育两种植物来进行该引入步骤。
65.一种增强根萤叶甲属昆虫种群的控制的方法，该方法包括提供如权利要求25所述的转基因植物或植物部分并且向该植物或植物部分施用化学杀有害生物剂。
66.如权利要求65所述的方法，其中该化学杀有害生物剂是氨基甲酸酯、拟除虫菊酯、有机磷酸酯、friprole、新烟碱、有机氯化物、沙蚕毒素或其组合。
67.如权利要求65所述的方法，其中该化学杀有害生物剂包含选自下组的活性成分，该组由以下组成：克百威、胺甲萘、灭多虫、联苯菊酯、七氟菊酯、氯菊酯、氟氯氰菊酯、λ-氯氟氰菊酯、氯氰菊酯、溴氰菊酯、毒死蜱、氯氧磷、乐果、灭线磷、马拉硫磷、甲基对硫磷、甲拌磷、特丁磷、叔丁嘧啶磷、氟虫腈、啶虫脒、吡虫啉、噻虫啉、噻虫嗪、硫丹、杀虫磺及其组合。
68.如权利要求67所述的方法，其中该活性成分在选自下组的产品中进行递送，该组由以下组成： 及其组合。
69.一种为玉米种植者提供控制玉米作物中的根萤叶甲属昆虫有害生物种群的手段的方法，该方法包括(a)向该种植者销售或为其提供包含本发明的干扰RNA分子、核酸分子、核酸构建体、和/或组合物的转基因玉米种子；并且(b)向该种植者做广告宣传该转基因玉米种子产生控制根萤叶甲属有害生物种群的转基因玉米植物。
70.一种鉴定直系同源靶基因用作控制植物有害生物的RNAi策略的方法，所述方法包括以下步骤：
a)产生将扩增选自下组的靶标的引物对，该组包含SEQ ID NO:31-90、397、398，或其互补序列，或由其组成，
b)扩增来自该植物有害生物的核酸样品的直系同源靶基因，
c)鉴定直系同源靶基因的序列，
d)产生干扰RNA分子，其中该RNA包含至少一种dsRNA，其中该dsRNA是包含退火的互补链的双链RNA的区域，其中的一条链包含具有至少19个连续核苷酸的序列，该序列与该靶基因中的直系同源靶核苷酸序列是至少部分互补的，以及
e)确定步骤(d)的干扰RNA分子是否对该植物有害生物具有杀昆虫活性；
其中如果该干扰RNA对该植物有害生物靶基因具有杀昆虫活性，则已鉴定出用于控制植物有害生物的直系同源靶基因。
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