CN109771352A - It is a kind of repair skin composition and its preparation cosmetics - Google Patents
It is a kind of repair skin composition and its preparation cosmetics Download PDFInfo
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- CN109771352A CN109771352A CN201910252999.1A CN201910252999A CN109771352A CN 109771352 A CN109771352 A CN 109771352A CN 201910252999 A CN201910252999 A CN 201910252999A CN 109771352 A CN109771352 A CN 109771352A
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Abstract
The present invention relates to compound technical more particularly to a kind of compositions for repairing skin and its cosmetics of preparation.Composition provided by the invention is made of extractive of flax seed, common milfoil extract.Composition provided by the invention can be realized the reparation on skin texture, realize the perfect of skin histology framework, it is additionally based on the reparation of nerve signal transmitting, promote cell differentiation, proliferation and migration, to not only repair and maintain skin texture in structure, effective transmitting of intercellular nerve signal more ensure that.The superposition of each component is acted synergistically using apparent synergy is generated, and improves the effect of skin barrier is repaired as a result, being significantly better than control group.
Description
Technical field
The present invention relates to compound technical more particularly to a kind of composition for repairing skin and its makeups of preparation
Product.
Background technique
The physiological structure of skin, including epidermis, basilar memebrane and skin corium and cellular neural communication etc., with people
There is aging phenomenon, show as wrinkle, relaxation, loss of elasticity, coarse, color in the continuous aging of body and the stimulation of outside, skin
Plain calm, atrophoderma and telangiectasis.And the aging phenomenon of these skins can be attributed to epidermis, basilar memebrane and true
What bring nerve communicated after the damage of cortex, and damage has some setbacks.The damage of epidermis and skin corium is embodied in cutin shape
At cell and fibroblastic decline and withered, this is also the point of more cosmetics concern at present, and the damage of basilar memebrane
It is not also many in current concern.The variation of basilar memebrane can generate far-reaching influence to corium and epidermis, basilar memebrane it is continuous
After property is destroyed, epidermal cell loses to be contacted with basilar memebrane, is directly connected with exposed corium, is influenced the knot of epidermis and corium
Structure support and the transport of nutriment, moisture.Epidermis is unable to get normal nutrition and moisture supply, and the keratinization of itself will
Influence is received, the missing of outermost layer protective layer-cuticula barrier function of skin is eventually led to.And basilar memebrane is impaired
Will lead to conduction of several growth factors in corium and epidermis, these conduction be obstructed also will further cause skin texture by
Damage.So repairing cuticula and skin corium, while promoting keratinocyte and fibroblast proliferation, it should also be noted that
The reparation of basilar memebrane, so that the structure of skin keeps integrality.In addition the MC-2R of cell differentiation and cell Proliferation is controlled in skin
It is constantly reduced with the expression quantity of MOR-1 neuroceptor, the expression quantity of neuroceptor reduces, it is meant that promote on kidney when cell issues
When gland cortin and endorphin, due to the missing of MC-2R and MOR-1 neuroceptor, cause differentiation, proliferation and the migration of cell by
Inhibit, is unable to complete the reparation of cell, is unable to complete self-regeneration so as to cause skin.Although most raw material has promotion
The effect of cell Proliferation, but only just can guarantee the cell of skin not only under conditions of reasonably adjusting neuroceptor
Be proliferation, and can normal differentiation and migration in the case where proliferation, to realize the integrality of skin texture.
And the integrality of skin is not limited only to the epidermis as physiologic barrier, also includes micro- life as biochemical barrier
Nitride layer, the microbial layer of skin are the ground stayed in that microbial flora is depended on for existence, and every square centimeter there are about 1,000,000,000 bacteriums peace
Coexist in the epidermis of skin, forms " protection shield " of Tiny ecosystem.The Tiny ecosystem of skin is by skin microbial, host skin and ring
The entity that border collectively forms.They mutually restrict, are mutually coordinated, keep dynamic equilibrium, are first of biological barriers of skin,
Any one ring, which is broken, in balance can all cause Dysbiosis.When such as skin environment exception or by outside stimulus, certain Chang Ding
Disease can also be caused in the flora on skin by planting.For example, propionibacterium acnes are the resident microorganisms of skin, but people pressure apprentice
When increasing causes face's fat secretion excessive, it will lead to propionibacterium acnes mass propagation, lead acnegenic generation, to cause
Disorganization.The excessive multiplication of propionibacterium acnes also leads to entire skin Dysbiosis, and the unbalance of Tiny ecosystem also will
Cause this outmost biochemical barrier of skin impaired, causes the physical barriers of skin to be degenerated, the invasion of harmful substance, thus right
Skin carries out deeper destruction.It therefore, not only will be in view of skin when the integrality in view of repairing skin
Structure, the i.e. reparation of the physiological structure of skin, it is also necessary to which in view of the reparation of microbial layer, i.e. the biochemical structure of skin is repaired
It is multiple.
The integrality of skin is the final goal that repairing type cosmetics are pursued.But reparation of the existing cosmetics to skin
Mechanism be more built upon skin structure reparation, i.e., be more the reparation for focusing on the cuticula of skin surface
Deng, have ignored in the reparation and skin texture of the microbial layer of skin surface it is otherwise reparation and cell nerve signal
The reparation of transmitting.
Summary of the invention
In view of this, the technical problem to be solved in the present invention is that providing a kind of composition for repairing skin and its preparation
Cosmetics, the composition can not only promote the proliferation of epidermal cell, additionally it is possible to inhibit harmful bacteria, and with replenishing water and preserving moisture
Effect.
Moisturizing of the present invention, moisturizing, antibacterial and/or promote the composition of cell Proliferation by the component of following mass parts
Composition:
0.0005~0.0075 part of extractive of flax seed；
0.0005~0.01 part of common milfoil extract.
In some embodiments, the composition is grouped as by the group of following mass parts:
1 part of extractive of flax seed；
1 part of common milfoil extract.
In some embodiments, the composition is grouped as by the group of following mass parts:
0.0075 part of extractive of flax seed；
0.01 part of common milfoil extract.
Composition provided by the invention is made of extractive of flax seed and common milfoil extract.The composition that the present invention uses
It can be realized the reparation on skin texture, realize the perfect of skin histology framework, be additionally based on the reparation of nerve signal transmitting, promote
Into cell differentiation, proliferation and migration, to not only repair and maintain skin texture in structure, more ensure that intercellular
Effective transmitting of nerve signal.The superposition of each component is acted synergistically using apparent synergy is generated, and improves skin barrier reparation
The effect of as a result, being significantly better than control group.
For simple cosmetic base, each object that combines generates significant moisturizing, moisturizing, antibacterial and/or promotion
The effect (p < 0.05) of cell Proliferation；Compared with the combination of constituent part, combined effect of the invention is more significant, illustrates each component
Compounding can generate good synergy synergistic effect.And compared with but the different control sample of proportion comparable with dosage, composition
Effect it is more significant, illustrate it is appropriate proportion be conducive in each component synergy act synergistically generation.
Application of the composition of the present invention in the cosmetics that skin physiology and/or biochemical structure are repaired in preparation.
In the present invention, described repair includes moisturizing, moisturizing, antibacterial and/or promotion cell Proliferation.
In the present invention, the moisturizing includes two aspect of instant moisturizing and long-acting moisturizing.It is provided in the embodiment of the present invention
Composition can the moisture content of skin at 30 minutes when increasing to 171.2%, 120 minutes moisturizing effect still have 167.3%
Left and right, instant moisturizing effect is obvious, and the duration is longer.It is lasting to use group of the invention from the point of view of long-acting moisturizing effect
Object is closed, the moisture content of skin increased about 13.14% at 28 days, continued to use, and the moisture content of skin can sustainable growth.
In the present invention, the moisturizing includes two aspect of instant moisturizing and long-acting moisturizing.From moisture of skin windage
From the point of view of reduction amount, moisture of skin windage can reduce using composition of the invention, when using 30 minutes, the water dispersion of skin
The reduction amount of vector reaches 11.5%.Continue to use composition of the invention in 28 days, the reduction amount of the water loss amount of skin reaches
To 13.31%, i.e. the water loss amount of skin is constantly being reduced, and the barrier function for embodying skin has obtained effectively repairing
It is multiple.
In the present invention, in the promotion cel l proliferation, the cell is fibroblast.From fibroblastic
From the point of view of cultivation effect, the present composition can effectively promote fibroblast proliferation, and proliferation rate has reached 124.40%, combination
Object proves that the composition can effectively improve the damage of skin corium in skin to the facilitation of fibroblast proliferation；To
Further being destroyed for skin texture effectively is prevented, improves the stability of skin, the physiological structure of skin is generated good
Repair.
In the embodiment of the present invention, the harmful bacteria for verifying antibacterial action is staphylococcus aureus.
The inhibiting rate of testing in vitro staphylococcus aureus the results show that composition of the invention to staphylococcus aureus
There are significant inhibiting effect.Composition of the invention can allow the bacterium of skin 6 hours after skin surface Tiny ecosystem is destroyed
It is horizontal to fall number restores to the clump count of skin after 61.82%, 12 hour to be restored to before bacterium colony destroys 90.91%.Separately
Outer composition is inhibited to the proliferation of the harmful staphylococcus aureus of skin, after 12h to the golden yellow of skin surface
Staphylococcic inhibiting rate maintains 60.87%.Composition proves that the composition can to the inhibiting effect of staphylococcus aureus
To repair skin surface flora, so that the biochemical structure to skin generates good repair.
The present invention also provides a kind of reparation skin physiology and/or biochemical structure cosmetics comprising of the present invention
Composition and cosmetic base；Wherein the mass fraction of composition of the present invention is 0.2%~3.5%.
The matrix of the cosmetics includes: lytic agent, moisturizer, penetrating agent, thickener and preservative；It is demonstrated experimentally that this
The cosmetic base that invention uses does not interfere the generation of composition effect, and can play to composition effect and preferably promote to make
With.
The lytic agent is water, glycerol and 1,3 butylene glycol；
In the lytic agent, glycerol and 1,3-BDO serve not only as lytic agent use, also have certain moisture-keeping function.
The mass ratio of the water, glycerol and 1,3 butylene glycol is (81.8545~82.9823): (15.03~15.45): (0.025~
0.5)。
The moisturizer is hyaluronic acid；Moisturizer of the present invention includes the hyaluronic acid that molecular weight is 2,100,000 Da
(high molecular weight hyaluronic acid) and molecular weight Jing Guo digestion are hyaluronic acid (the small molecule hyalomitome of 1000~5000Da
Acid).The mass ratio of the high molecular weight hyaluronic acid and micromolecule hyaluronic acid is 1:20.Wherein, the hyalomitome of macromolecule
Acid acts on skin surface, and micromolecule hyaluronic acid is able to enter inside skin, plays the role of replenishing water and preserving moisture.
The penetrating agent is hydroxyethyl piperazine ethane sulfonic acid and pentanediol；
Penetrating agent is also known as transdermal absorption accelerator (penetration enhancers), can reduce active material and passes through
The resistance of skin accelerates active material to penetrate the substance of skin.The penetrating agent that the present invention selects is harmless to skin skin or pierces
Swash.In the cosmetics, the mass fraction of penetrating agent is 0.41%~0.6%.
The thickener is xanthan gum；The thickener can increase the density of cosmetics.It is thickened in the cosmetics
The mass fraction of agent is 0.0005%~0.01%.
The preservative is parahydroxyacet-ophenone, Phenoxyethanol and sodium benzoate.Preservative is for inhibiting in cosmetics
Microbial growth extends the storage life of cosmetics.The mass fraction of Determination of Preservatives in Cosmetics is 0.5012%~0.518%.Its
Described in parahydroxyacet-ophenone, Phenoxyethanol and sodium benzoate mass ratio be 0.5:(0.0009~0.0135): (0.0003~
0.0045)。
In some embodiments, cosmetics of the present invention are grouped as by the group of following mass fraction:
In some specific embodiments, cosmetics of the present invention are grouped as by the group of following mass fraction:
In some specific embodiments, cosmetics of the present invention are grouped as by the group of following mass fraction:
In some specific embodiments, cosmetics of the present invention are grouped as by the group of following mass fraction:
Cosmetics of the present invention are lotion, stoste, Essence, toner, facial mask, cream, mildy wash etc..The present invention is real
It applies in example, the cosmetics are stoste.
The preparation method of cosmetics of the present invention, comprising:
Prepare the mother liquor A of extractive of flax seed: weighing extractive of flax seed, mixed with the glycerol of 60 times of quality, 25 DEG C with
250~300r/min speed stirring and dissolving, is added sodium benzoate and Phenoxyethanol, after stirring and dissolving, water is added and is diluted to flax
The mass fraction that seed extracts is 0.5%；
Prepare the mother liquor B of common milfoil extract: weigh common milfoil extract with etc. quality xanthan gum, be added common milfoil mention
The water of 128 times of quality of object is taken, 25 DEG C are stirred, addition 1,3-BDO and penta two transparent to dissolving with 250~300r/min speed
Alcohol, the mass fraction for being diluted to common milfoil extraction is 0.5%；
Weigh 2,100,000 hyaluronic acids, digestion hyaluronic acid, hydroxyethyl piperazine ethane sulfonic acid, fructose and inulin, addition go from
Sub- water is heated to 75~80 DEG C, with 250~300r/min, stirring and dissolving 20min；
Glycerol and parahydroxyacet-ophenone is added, temperature control is 65~75 DEG C, with 300r/min, stirring and dissolving 15min；
The mother liquor A, mother liquor B are sequentially added, temperature controls 45 DEG C hereinafter, with 100r/min, stirs 15min, be cooled to
Cosmetics are made in room temperature.
Heretofore described room temperature is 18~30 DEG C.
It is that external application is of the present invention the present invention also provides a kind of method of reparation skin physiology and/or biochemical structure
Cosmetics.The external application is to smear, spray, sticking.
Composition provided by the invention is made of extractive of flax seed, common milfoil extract.Composition provided by the invention
It can be realized the reparation on skin texture, realize the perfect of skin histology framework, be additionally based on the reparation of nerve signal transmitting, promote
Into cell differentiation, proliferation and migration, to not only repair and maintain skin texture in structure, more ensure that intercellular
Effective transmitting of nerve signal.The superposition of each component is acted synergistically using apparent synergy is generated, and improves skin barrier reparation
The effect of as a result, being significantly better than control group.
Detailed description of the invention
The instant moisturizing effect of Fig. 1 patent portfolios；
The long-acting moisturizing effect of Fig. 2 patent portfolios；
The reduction amount of the instant moisture of skin windage of Fig. 3 patent portfolios；
The reduction amount of Fig. 4 patent portfolios long lasting skin water loss amount；
Fig. 5 fibroblast cell proliferation rate；
The recovery effects of Fig. 6 normal skin flora；
The inhibitory effect of Fig. 7 skin staphylococcus aureus.
Specific embodiment
The present invention provides a kind of compositions for repairing skin and its cosmetics of preparation, those skilled in the art to borrow
Reflect present disclosure, is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to this field
It is it will be apparent that they are considered as being included in the present invention for technical staff.Method and application of the invention has passed through
Preferred embodiment is described, related personnel obviously can not depart from the content of present invention, in spirit and scope to the side of this paper
Method and application are modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
The test material that the present invention uses is all common commercially available product, can all be bought in market.
Extractive of flax seed is that the dry mature seed of flax family (Linaceae) plant flax (Linum usitatissimum) is extracted
It hydrolyzes and is made after protein, be rich in a large amount of plant polypeptide.The present invention promotes the synthesis of antibacterial peptide using extractive of flax seed.
Common milfoil extract extracts from the aerial part of common milfoil (Achillea millefolium), and active constituent is Huang
Letones can promote the expression of MC-2R and MOR-1, the transmitting of the nerve signal between skin inner cell be repaired, to promote
Cell Proliferation, migration and differentiation are finally reached the target for repairing skin physiology structure.
The stoste of heretofore described cosmetics, which refers to, adds relatively single high concentration skin care ingredient, can be directed to various fleshes
Skin needs, maintenance more direct to skin, safer, more potent, and the beauty for allowing skin to restore optimum state in a short time produces
Product, full ingredient is no more than 20 in principle.
Below with reference to embodiment, the present invention is further explained:
Examples 1 to 3
1 Examples 1 to 3 prescription of table (unit: g)
Preparation method includes:
1, the mother liquor A of extractive of flax seed is prepared by the way of cold match.Concrete operations are to weigh 0.5% linseed to mention
Take object concentrate, 30% glycerol be added, stirred with 250-300r/min speed to transparent, be added 0.3% sodium benzoate and
0.9% Phenoxyethanol after stirring and dissolving, is added 68.3% deionized water and is diluted.It is refrigerated after finally filtering spare.
2, the mother liquor B of common milfoil extract is prepared by the way of cold match.Concrete operations are to weigh 0.5% common milfoil to mention
64% deionized water is added in the xanthan gum for taking object concentrate and 0.5%, is stirred with 250-300r/min speed saturating to dissolution
It is bright, 25% 1,3-BDO and 10% pentanediol is added, stirs evenly.It is refrigerated after finally filtering spare.
3, according to 1 prescription of table weigh 2,100,000 hyaluronic acids, digestion hyaluronic acid, hydroxyethyl piperazine ethane sulfonic acid, fructose and
Inulin is added deionized water and is heated to 75-80 DEG C, with 250-300r/min, stirs 20min, until dissolving transparent；
4,15g glycerol and parahydroxyacet-ophenone being added according to 1 prescription of table, temperature control is 65-75 DEG C, with 300r/min,
15min is stirred, until all additives all dissolve；
5, mother liquor A, mother liquor B being sequentially added according to prescription, temperature controls 45 DEG C hereinafter, with 100r/min, stirs 15min,
Standing is restored to room temperature.
Comparative example 1~3
2 comparative example of table, 1~3 prescription (unit: g)
Preparation method includes:
1, the mother liquor A of extractive of flax seed is prepared by the way of cold match.Concrete operations are to weigh 0.5% linseed to mention
Take object concentrate, 30% glycerol be added, stirred with 250-300r/min speed to transparent, be added 0.3% sodium benzoate and
0.9% Phenoxyethanol after stirring and dissolving, is added 68.3% deionized water and is diluted.It is refrigerated after finally filtering spare.
2, the mother liquor B of common milfoil extract is prepared by the way of cold match.Concrete operations are to weigh 0.5% common milfoil to mention
64% deionized water is added in the xanthan gum for taking object concentrate and 0.5%, is stirred with 250-300r/min speed saturating to dissolution
It is bright, 25% 1,3-BDO and 10% pentanediol is added, stirs evenly.It is refrigerated after finally filtering spare.
3, according to 1 prescription of table weigh 2,100,000 hyaluronic acids, digestion hyaluronic acid, hydroxyethyl piperazine ethane sulfonic acid, fructose and
Inulin is added deionized water and is heated to 75-80 DEG C, with 250-300r/min, stirs 20min, until dissolving transparent；
4,15g glycerol and parahydroxyacet-ophenone being added according to 1 prescription of table, temperature control is 65-75 DEG C, with 300r/min,
15min is stirred, until all additives all dissolve；
5, mother liquor A, mother liquor B being sequentially added according to prescription, temperature controls 45 DEG C hereinafter, with 100r/min, stirs 15min,
Standing is restored to room temperature.
Effect evaluation and test
1) the instant and long-acting moisturizing effect of skin
Test equipment: German CK company Multi-functional skin tester, model C orneometer CM825MDD,
Test philosophy: using the moisture content of capacitance method test human body keratoderma, its principle is based on water and its
His difference in dielectric constant of substance is significant, and according to moisture content of skin difference, the capacitance for measuring skin is different, and observed parameter can
Represent skin moisture value.
Test environment: test environment temperature is 22 ± 1 DEG C, and humidity is 50 ± 5%, and carries out Real-time and Dynamic Detection；
Test volunteer: effective volunteer at least 30 people, the age, (gestation or the newborn phase women in Pu removed between 16-65 years old
Outside)；Forearm test zone capacitance method moisture of skin measures the basic value of meaning between 15-100；Without serious systemic disease, nothing
Immune deficiency or autoimmune disease person；Previously to skin-care cosmetics without severe allergy history person；Do not made in nearly one month
With hormone medicine and immunosuppressor person；Other clinical test persons are not participated in；Use according to regulations test medicine and all information；
All volunteers should fill in informed consent form before testing.
Testing procedure:
Prepare before test: any product (cosmetics or topical drug) cannot be used within 2-3 days before recipient site.Before experiment,
Subject needs to agree to survey in cleaning both hands forearm, natural air drying.It is surveyed in subject's both hands forearm after cleaning and carries out measured zone
Label.At least 30min that sits quietly in room should be being complied with standard before official testing, cannot drunk water, forearm exposure is put in test mode
It sets, keeps loosening.
Test process: 3 × 3cm of label on the inside of left and right arm in experiment2Test area, same arm can mark multiple areas
Domain, it is interregional every 1cm.Test product and blank control are randomly dispersed in left and right arm.Use probe Corneometer
CM825MDD carries out the skin moisture content measurement of tested region and control zone.Each region is according to parallel determination 15 times.First
The blank value of each test zone is measured, 0.072ml sample/cm is then pressed2Dosage, inject a sample into film cloth, and apply
In trial zone, the time is 20 minutes.It takes facial mask cloth off after twenty minutes, tests the area skin moisture content after ten minutes, as
The moisture content of skin at 30 minutes.It measures respectively later 1 hour, 2 hours, the skin in tested region and blank control region contained
Water (is measured when verifying by this time), and the test of the same volunteer is completed by the same survey crew.
For the test of long-acting moisturizing, subject voluntarily carries out smearing survey according to above-mentioned test method in the same area daily
Test agent, and the moisture content of skin was tested in the case where not smearing sample to be tested at the 14th, 28 day.
Test data: the skin moisture content of each period is measured respectively by the design of experiment, and calculates various time points
Skin moisture content incrementss.
Experimental result:
1.1) result of moisturizing immediately
The instant moisturizing effect of 3 patent portfolios of table
As a result: being formulated from the point of view of instant moisturizing effect, based on control sample -1 has certain moisturizing effect, and 30 minutes
When moisture content incrementss be 127.7%.The moisture content of the skin at 30 minutes of example sample -1 increases to 171.2%, and 120 points
Moisturizing effect still has 167.3% when clock, and instant moisturizing effect is obvious, and the duration is longer.
1.2) long-acting moisturizing effect
The long-acting moisturizing effect of 4 patent portfolios of table
As a result: from the point of view of long-acting moisturizing effect, when lasting use example sample -1, the moisture content of skin has one
The process of growth, at 28 days, the moisture content of skin increased about 13.14%.Also the barrier function for having reacted skin of side simultaneously
It can be repaired, the moisture content of skin is made to get a promotion.
Relative to control group 1, the moisturizing effect of each experimental group is more significant (p < 0.05), illustrates that composition of the invention exists
Significant moisturizing effect；
Compared with control group 2, the moisturizing effect of Examples 1 to 3 is more significant (p < 0.05), illustrates that multi-component combination generates
More significant synergy synergistic effect, effect are better than the combination of part of component；
Compared with control group 3, for the moisturizing effect of example sample 1~3 there are statistical difference (p < 0.05), explanation need to be specific
Under proportion, each component could generate better synergy synergistic effect；
Relative to example sample 1~2, the moisturizing effect of example sample 3 is more significant (p < 0.05), illustrates under concentration appropriate, group
The moisturizing effect for closing object is more significant.
2) the barrier repairing effect (TEWL value) of skin
Test equipment: German CK company Multi-functional skin tester, pop one's head in model Tewameter TM300,
Test philosophy: FICK Fick's law of diffusion: dm/dt=D.A.dp/dx.It is surveyed by two groups of temperature-humidity sensors
The water vapor pressure gradient that fixed nearly epidermis (about in 1cm) is formed by cuticula moisture loss in different bright spots directly measures percutaneous dissipate
The amount of moisture of hair.TEWL value is an important symbol of skin barrier quality, and the TEWL value of skin is lower, illustrates the barrier of skin
Function is better, otherwise poorer.
Test environment: test environment temperature is 22 ± 1 DEG C, and humidity is 50 ± 5%, and carries out Real-time and Dynamic Detection；
Test volunteer: effective volunteer at least 30 people, the age, (gestation or the newborn phase women in Pu removed between 16-65 years old
Outside)；Without serious systemic disease, no immune deficiency or autoimmune disease person；Previously to skin-care cosmetics without severe allergy
Shi Zhe；Hormone medicine and immunosuppressor person are not used in nearly one month；Other clinical test persons are not participated in；Make by regulation
With test medicine and all information；All volunteers should fill in informed consent form before testing.
Testing procedure:
Prepare before test: any product (cosmetics or topical drug) cannot be used within 2-3 days before recipient site.Before experiment,
Subject needs to agree to survey in cleaning both hands forearm, natural air drying.It is surveyed in subject's both hands forearm after cleaning and carries out measured zone
Label.At least 30min that sits quietly in room should be being complied with standard before official testing, cannot drunk water, forearm exposure is put in test mode
It sets, keeps loosening.
Test process: 3 × 3cm of label on the inside of left and right arm in experiment2Test area, same arm can mark multiple areas
Domain, it is interregional every 1cm.Test product and blank control are randomly dispersed in left and right arm.Use probe Tewameter
TM300 carries out the lost measurement of moisture of skin in tested region and control zone.Each region is according to parallel determination 15 times.First survey
The blank value of each test zone is measured, 0.072ml sample/cm is then pressed2Dosage, inject a sample into film cloth, and apply and trying
It tests in area, the time is 20 minutes.It takes facial mask cloth off after twenty minutes, tests the area skin moisture loss content after ten minutes, i.e.,
The water loss amount of skin when being 30 minutes.Later respectively measure 1 hour, 2 hours, the skin in tested region and blank control region
The test of skin water loss amount, the same volunteer is completed by the same survey crew.
For the test of long-acting barrier reparation, subject voluntarily carries out in the same area according to above-mentioned test method daily
Test sample is smeared, and tested the water loss amount of skin in the case where not smearing sample to be tested at the 14th, 28 day
TEWL。
Test data: it measures the moisture of skin windage of each period respectively by the design of experiment, and calculates each time
The reduction amount of the moisture of skin windage of point.The reduction amount of moisture of skin windage is bigger, and the effect of skin barrier reparation is better.
Experimental result:
2.1) instant barrier repairing effect
The reduction amount of the instant moisture of skin windage of table 5
As a result: from the point of view of the reduction amount of moisture of skin windage, control sample -1 is that basic sample has certain reduction skin
The effect of water loss amount, at 30 minutes, the water loss amount of skin is reduced to 5.9%, the moisture of the skin of example sample -1
The reduction amount of windage reaches 11.5%, significantly reduces the water loss amount of skin, embodies repairing for instant skin barrier
Multiple effect.
2.2) long-acting barrier repairing effect
The reduction amount of 6 long lasting skin water loss amount of table
As a result: from the point of view of the result of the reduction amount of long-acting moisture of skin windage, continue the use of 28 days example samples -1,
So that the reduction amount of the water loss amount of skin reaches 13.31%, i.e. the water loss amount of skin is constantly being reduced, and is embodied
The barrier function of skin has obtained effective reparation.
Relative to control group 1, the moistening effect of each experimental group is more significant (p < 0.05), illustrates that composition of the invention exists
Significant moistening effect；
Compared with control group 2, the moistening effect of Examples 1 to 3 is more significant (p < 0.05), illustrates that multi-component combination generates
More significant synergy synergistic effect, effect are better than the combination of part of component；
Compared with control group 3, for the moistening effect of example sample 1~3 there are statistical difference (p < 0.05), explanation need to be specific
Under proportion, each component could generate better synergy synergistic effect；
Relative to example sample 1~2, the moistening effect of example sample 3 is more significant (p < 0.05), illustrates under concentration appropriate, group
The moistening effect for closing object is more significant.
3) fibroblast proliferation effect-MTT decoration method
Experimental principle
MTT full name is 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyl-2-H-tetrazolium
Bromide, entitled 3- (4,5- dimethylthiazole -2) -2, the 5- diphenyltetrazolium bromide bromide of Chinese chemistry, trade name: thiazolyl blue,
It is a kind of dyestuff of yellow color.Mtt assay is also known as MTT colorimetric method, is a kind of method for detecting cell survival and growth.It detects former
Reason is that the bluish violet that the succinate dehydrogenase in living cells mitochondria can make exogenous MTT be reduced to water-insoluble crystallizes first a ceremonial jade-ladle, used in libation
(Formazan) it and is deposited in cell, and dead cell is without this function.Dimethyl sulfoxide (DMSO) can dissolve the first a ceremonial jade-ladle, used in libation in cell,
Its absorbance value is measured at 540 or 720nm wavelength with enzyme-linked immunosorbent assay instrument, can reflect living cells quantity indirectly.Certain
Within the scope of cell number, it is directly proportional to cell number that MTT crystallizes the amount to be formed.
Experimental material and method
Experimental material: DMEM, DPBS, Typsin-EDTA, Petri dish, 96well dish, 0.4% trypan blue are molten
Liquid, dehydrated alcohol or 95% ethanol solution, simple microscope, cell counting board, liquid-transfering gun, MTT, DMSO
Experimental method:
1. Fibroblast cell-culture: it is collected after cultured fibroblast is handled with Typsin-EDTA, it is outstanding with DMEM
Suspension is diluted to the concentration of cell spare for 5 × 104cells/ml after counting after floating using blood cell counting plate.It will prepare
Good cell suspending liquid is distinguished plant division and is cultivated into 96well dish and 6well dish, and wherein 96well dish inoculum concentration is
100ul, 6well dish inoculum concentration are 2ml.At 37 DEG C, 5%CO2Incubator in cultivate 24 hours.
2. sample adds: sample to be tested is trained base with DMEM and is diluted, and the concentration after dilution is respectively as follows: 1% (before the concentration passes through
The MTT in face is tested, and result is nontoxic).After cell culture 24 hours, DMEM before removing, after then carefully being washed with DPBS.
The DMEM culture medium for being added to sample to be tested that the first step prepares is added in sequence.At 37 DEG C, 5%CO2Incubator in train
It supports 48 hours.
3.MTT dyeing: the MTT powder for freezing keeping is dissolved using DPBS, is configured to the solution that concentration is 2mg/ml.48 is small
When culture after 96well dish in, remove culture medium after, respectively add 50ul/well MTT solution.At 37 DEG C, 5%CO2
Incubator in cultivate 3 hours.MTT solution is removed completely after 3 hours.MTT solution removes completely to be added respectively in rear each well
The DMSO solution of 100ul/well is added to make bluish violet crystallization first a ceremonial jade-ladle, used in libation (formazan crystal) dissolution.Extinction is surveyed at 595nm
Luminosity.On the basis of blank sample, the cell proliferation rate of each sample is calculated.
Experimental result:
7 fibroblast proliferation rate of table
| Fibroblast proliferation rate (%) | Standard deviation | |
| Control sample -1 | 98.82 | 4.3 |
| Control sample -2 | 114.62 | 3.2 |
| Control sample -3 | 101.42 | 4.8 |
| Example sample -1 | 124.40 | 2.4 |
| Example sample -2 | 120.54 | 4.7 |
| Example sample -3 | 130.50 | 3.8 |
As a result: from the point of view of fibroblastic cultivation effect, example sample -1 can effectively promote fibroblastic proliferation,
Proliferation rate has reached 124.40%, and fibroblastic proliferation can effectively improve the damage of skin corium in skin, to have
Effect prevents further being destroyed for skin texture, to improve the stability of skin.
Relative to control group 1, the effect of the promotion cell Proliferation of each experimental group is more significant (p < 0.05), illustrates of the invention
There is the significant effect for promoting cell Proliferation in composition；
Compared with control group 2, the effect of the promotion cell Proliferation of Examples 1 to 3 is more significant (p < 0.05), illustrates multicomponent
Combination generate more significant synergy synergistic effect, effect is better than the combination of part of component；
Compared with control group 3, the effect of the promotion cell Proliferation of example sample 1~3 is said there are statistical difference (p < 0.05)
It is bright need to be under specific proportion, each component could generate better synergy synergistic effect；
Relative to example sample 1~2, the effect of the promotion cell Proliferation of example sample 3 is more significant (p < 0.05), illustrates appropriate
Under concentration, the effect of the promotion cell Proliferation of composition is more significant.
4) recovery effects (total plate count detection method) of skin Tiny ecosystem
Experimental principle: the measuring method of living microorganisms quantity commonly uses plate culture counting method.It is by by sample
A series of dilution of uniform different dilutions is made, then certain dilution is taken to be inoculated with, it is made to be uniformly distributed in culture dish
In in specific culture medium, the last viable bacteria calculated according to the clump count grown on plate in every gram of (or every milliliter) sample
Quantity.The acquisition for carrying out skin bacterium colony in this test in the fixed area of skin using the cotton swab of inactivation, by cotton swab after acquisition
It is soaked in the physiological saline of inactivation, the measurement of living microorganisms quantity is carried out after concussion dispersion.
Laboratory apparatus: 90mm culture dish, sterile conical flask, liquid-transfering gun and pipette tips, autoclave, constant incubator, apply stick,
Alcolhol burner, superclean bench, magnetic stirring apparatus, vortex oscillator, cotton swab
Experimental material: physiological saline, 75% alcoholic solution, lecithin, Tween 80-nutrient agar
Medium component: it peptone 20g, beef extract 3g, sodium chloride 5g, agar 15g, lecithin 1g, Tween 80 7g, steams
Distilled water 1000ml.
Lecithin: being first added in a small amount of distilled water by preparation method, dissolves by heating, and Tween 80 is added, and other compositions (are removed agar
It is added in remaining distilled water, dissolves outside).Lecithin, the Tween 80 dissolved is added, mixes, adjusting pH value is 7.1~7.4,
Agar is added, it is spare to be stored in dark cold place by 121 DEG C of high pressure sterilization 20min.
Experimental method:
1, left and right arm inside 3 × 3cm of label in experiment2Test area, same arm can mark multiple regions, region
It is spaced 1cm.Test product and blank control are randomly dispersed in left and right arm.
2,6 regions are chosen, the cotton swab of each region sterilizing dips after a small amount of physiological saline wipes 50 times back and forth, by cotton
Label are dipped in the physiological saline of same volume, are shaken with vortex oscillator, bacterium is distributed in physiological saline.The sample
The clump count initial as skin, is denoted as A0；Wherein the quantity of staphylococcus aureus is B0.
2,6 regions of inboard arm, respectively with 3 × 3cm2The covering of film cloth, 0.4ml75% is injected in the film cloth later
Alcoholic solution sterilizes.After sterilizing, the cotton swab of each region sterilizing is dipped after a small amount of physiological saline wipes 50 times back and forth, will
Cotton swab is dipped in the physiological saline of same volume, is shaken with vortex oscillator.The sample is whether detection bacterium is killed.
3, after 6 region sterilizings of inboard arm, respectively with 3 × 3cm2Film cloth covering, later in each film cloth respectively
Corresponding patent sample is injected, simulation facial mask applies removes membrane removal cloth after twenty minutes
4, it is dipped respectively with the cotton swab of sterilizing after a small amount of physiological saline wipes 50 times back and forth every 6 hours, 12 hours later, it will
Cotton swab is dipped in the physiological saline of same volume, is shaken with vortex oscillator, and bacterium is distributed in physiological saline.The sample
Recovery value of the product as skin clump count, is denoted as A1, and wherein the quantity of staphylococcus aureus is B1.
5,10 multiplying factor dilute sample
6, it will melt and be cooled to 45 DEG C -50 DEG C of lecithin tween 80 nutrient agar culture medium and be poured into culture dish, often
A culture dish about 15ml, rotates culture dish immediately, is sufficiently mixed sample and culture medium uniformly, after agar solidification, overturning training
Ware is supported, is placed in 36 DEG C of incubators and cultivates 48h.
7, bacterium colony count: first detect by an unaided eye, count clump count, then use 5~10 times of lens examinations, to prevent omission.
After the clump count for writing down each culture dish, the average colony number of each culture dish growth of same dilution is found out.
8, the recovery rate of the bacterium colony of skin is calculated by following equation:
Recovery effect=[(A0-A1)/A0] × 100%；
The inhibiting rate of staphylococcus aureus:
S.Aureus Inhibition ratio=[(B0-B1)/B0] × 100%
Experimental result:
The recovery effects of 8 normal skin flora of table
As a result: from the recovery effects of skin flora it can be found that the clump count of impaired skin can be quickly restored to
Clump count before impaired.Example sample -1 can be allowed at 6 hours after Tiny ecosystem is destroyed the clump count of skin restore to
The clump count of skin can be restored to before bacterium colony destroys after 61.82%, 12 hours 90.91% is horizontal, and control sample -1 exists
It can only restore 78.90% after 12 hours.In addition it is inhibited to the proliferation of the harmful staphylococcus aureus of skin, example sample -1
60.87% is maintained to the inhibiting rate of the staphylococcus aureus of skin surface after 12h.
Relative to control group 1, the fungistatic effect of each experimental group is more significant (p < 0.05), illustrates that composition of the invention exists
Significant fungistatic effect；
Compared with control group 2, the fungistatic effect of Examples 1 to 3 is more significant (p < 0.05), illustrates that multi-component combination generates
More significant synergy synergistic effect, effect are better than the combination of part of component；
Compared with control group 3, for the fungistatic effect of example sample 1~3 there are statistical difference (p < 0.05), explanation need to be specific
Under proportion, each component could generate better synergy synergistic effect；
Relative to example sample 1~2, the fungistatic effect of example sample 3 is more significant (p < 0.05), illustrates under concentration appropriate, group
The fungistatic effect for closing object is more significant.
The above is only the preferred embodiment of the present invention, it is noted that those skilled in the art are come
It says, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications also should be regarded as
Protection scope of the present invention.
Claims (9)
1. a kind of moisturizing, moisturizing, antibacterial and/or the composition for promoting cell Proliferation, are grouped as by the group of following mass parts:
0.0005~0.0075 part of extractive of flax seed；
0.0005~0.01 part of common milfoil extract.
2. composition according to claim 1, which is characterized in that be grouped as by the group of following mass parts:
1 part of extractive of flax seed；
1 part of common milfoil extract.
3. composition according to claim 1, which is characterized in that be grouped as by the group of following mass parts:
0.0075 part of extractive of flax seed；
0.01 part of common milfoil extract.
4. the described in any item compositions of claims 1 to 3 are in the cosmetics that skin physiology and/or biochemical structure are repaired in preparation
Application.
5. a kind of cosmetics for repairing skin physiology and/or biochemical structure comprising cosmetic base and claims 1 to 3 are appointed
Composition described in one；Wherein the mass fraction of the described in any item compositions of claims 1 to 3 is 0.2%~3.5%.
6. cosmetics according to claim 5, which is characterized in that the matrix of the cosmetics includes: lytic agent, moisturizing
Agent, penetrating agent, thickener and preservative；
The lytic agent is water, glycerol and 1,3 butylene glycol；
The moisturizer is hyaluronic acid；
The penetrating agent is hydroxyethyl piperazine ethane sulfonic acid and pentanediol；
The thickener is xanthan gum；
The preservative is parahydroxyacet-ophenone, Phenoxyethanol and sodium benzoate.
7. cosmetics according to claim 6, which is characterized in that the macromolecule that the hyaluronic acid is 1:20 by mass ratio
It measures hyaluronic acid and micromolecule hyaluronic acid forms.
8. cosmetics according to claim 5 or 6, which is characterized in that be grouped as by the group of following mass fraction:
9. the preparation method of claim 5~8 cosmetics, comprising:
Prepare the mother liquor A of extractive of flax seed: weighing extractive of flax seed, mixed with the glycerol of 60 times of quality, 25 DEG C with 250~
300r/min speed stirring and dissolving, is added sodium benzoate and Phenoxyethanol, after stirring and dissolving, water is added and is diluted to linseed extraction
Mass fraction be 0.5%；
Prepare the mother liquor B of common milfoil extract: weigh common milfoil extract with etc. quality xanthan gum, be added common milfoil extract
The water of 128 times of quality, 25 DEG C are stirred, addition 1,3-BDO and pentanediol transparent to dissolving with 250~300r/min speed, dilute
Releasing the mass fraction extracted to common milfoil is 0.5%；
2,100,000 hyaluronic acids, digestion hyaluronic acid, hydroxyethyl piperazine ethane sulfonic acid, fructose and inulin are weighed, deionized water is added
75~80 DEG C are heated to, with 250~300r/min, stirring and dissolving 20min；
Glycerol and parahydroxyacet-ophenone is added, temperature control is 65~75 DEG C, with 300r/min, stirring and dissolving 15min；
The mother liquor A, mother liquor B are sequentially added, temperature controls 45 DEG C hereinafter, with 100r/min, stirs 15min, be cooled to room temperature
Cosmetics are made.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110448478A (en) * | 2019-09-26 | 2019-11-15 | 湖南御家化妆品制造有限公司 | Composition and application thereof in preparing skin care product for regulating biological rhythm of skin |
| CN110538111A (en) * | 2019-09-26 | 2019-12-06 | 湖南御家化妆品制造有限公司 | Composition and application thereof in preparation of skin product for caring skin of person staying up at night |
| CN111991309A (en) * | 2020-08-20 | 2020-11-27 | 广州优理氏生物科技有限公司 | Composition with repairing and moisturizing effects and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100513082B1 (en) * | 2002-11-27 | 2005-09-07 | 나드리화장품주식회사 | Cosmetic composition containing ceramide liposome with phyto complex extract and ceramide |
| CN104884074A (en) * | 2012-10-26 | 2015-09-02 | Isp投资公司 | Use of a flax extract, originating from the hydrolysis of flax proteins, as an active antimicrobial agent |
-
2019
- 2019-03-29 CN CN201910252999.1A patent/CN109771352A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100513082B1 (en) * | 2002-11-27 | 2005-09-07 | 나드리화장품주식회사 | Cosmetic composition containing ceramide liposome with phyto complex extract and ceramide |
| CN104884074A (en) * | 2012-10-26 | 2015-09-02 | Isp投资公司 | Use of a flax extract, originating from the hydrolysis of flax proteins, as an active antimicrobial agent |
Non-Patent Citations (4)
| Title |
|---|
| PAIN，S ET AL: "Ability to Achillea millefolium and Prunus spinosa to increase the expression of MC-2R and MOR-1 inkeratinocytes and improve epidermal differentiation", 《JOURNAL OF INVESTIGATIVE DERMATOLOGY》 * |
| 上海禾泊秀化妆品有限公司: "禾泊秀 绿茶男士平衡精华乳", 《HTTP://FTBA.NMPA.GOV.CN:8181/FTBAN/ITOWNET/HZP_BA/FW/PZ.JSP?PROCESSID=201609211447210HTED&NID=201609211447210HTED》 * |
| 欧朵（上海）化妆品有限公司: "花肌粹 蓝莲净润保湿面膜", 《HTTP://FTBA.NMPA.GOV.CN:8181/FTBAN/ITOWNET/HZP_BA/FW/PZ.JSP?PROCESSID=20170703130051AUESF&NID=20170703130051AUESF》 * |
| 王建新等: "《化妆品植物原料大全》", 30 June 2012, 中国纺织出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110448478A (en) * | 2019-09-26 | 2019-11-15 | 湖南御家化妆品制造有限公司 | Composition and application thereof in preparing skin care product for regulating biological rhythm of skin |
| CN110538111A (en) * | 2019-09-26 | 2019-12-06 | 湖南御家化妆品制造有限公司 | Composition and application thereof in preparation of skin product for caring skin of person staying up at night |
| CN110448478B (en) * | 2019-09-26 | 2021-04-23 | 湖南御家化妆品制造有限公司 | Composition and application thereof in preparing skin care product for regulating biological rhythm of skin |
| CN110538111B (en) * | 2019-09-26 | 2021-04-23 | 湖南御家化妆品制造有限公司 | Composition and application thereof in preparation of skin product for caring skin of person staying up at night |
| CN111991309A (en) * | 2020-08-20 | 2020-11-27 | 广州优理氏生物科技有限公司 | Composition with repairing and moisturizing effects and preparation method and application thereof |
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Address after: 410000 No. 390, Guyuan Road, Changsha hi tech Development Zone, Changsha City, Hunan Province Applicant after: Shuiyang Cosmetics Manufacturing Co.,Ltd. Address before: No.668, Qingshan Road, Changsha high tech Development Zone, Changsha, Hunan Province Applicant before: HUNAN YUJIA COSMETICS MANUFACTURING Co.,Ltd. |
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Application publication date: 20190521 |