CN110123700A - A kind of eye cream releived eye circumference wrinkle and improve skin dimness - Google Patents
A kind of eye cream releived eye circumference wrinkle and improve skin dimness Download PDFInfo
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- CN110123700A CN110123700A CN201910518203.2A CN201910518203A CN110123700A CN 110123700 A CN110123700 A CN 110123700A CN 201910518203 A CN201910518203 A CN 201910518203A CN 110123700 A CN110123700 A CN 110123700A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
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Abstract
The invention discloses a kind of eye cream releived eye circumference wrinkle and improve skin dimness, the eye cream mainly contains silk tree (ALBIZIA JULIBRISSIN) bark extract and edelweiss (LEONTOPODIUM ALPINUM) callus extract.With eye circumference skin moisture and nutrition is replenished in time, eye circumference of releiving in time wrinkle and improve that skin is dull, the skin quality such as dry and astringent, moistens and nourish, promotion eye circumference skin maintenance effect.
Description
Technical field
The present invention relates to cosmetic fields, and in particular to a kind of eye cream releived eye circumference wrinkle and improve skin dimness.
Background technique
Eye part skin is especially soft and fine slim, is most thin one of the skin of human body, and thickness is only the 1/3-1/5 of skin of face,
Excessively thin skin corium lacks flexibility fiber and collagen structure, is easy to follow the string because of fatigue to become loose, and then generate wrinkle.
In addition, being influenced with advancing age and by environmental stimuli, the free radical that dissociates in skin is more and more, and free radical destroys
Collagen, active material in normal cell membrane tissue make the speed that collagen and elastic fibers generate in dermal layer of the skin
Degree is unable to catch up with the speed being lost, and loses mesh-supported power, and Elastic fibers and collagenous fibres change and gradually lose skin
Elasticity and tension cause eye skin relaxation.
Secondly, people blinks about 10000 times daily and eye circumference is affected to enclose 14 groups of muscle, to realize this high-frequency movement,
Circumference of eyes is dispersed with the very high nerve fibre of density and capillary, when eye is because of bad life habits over fatigue, such as
It stays up late, the electronic products such as computer cell phone is used for a long time, chronic sun exposure, smokes, drink, the factors such as emotional disturbance, will lead to blood
Recycle it is unsmooth cause extravasated blood, occur that eye is dim, pigmentation.
Therefore, eye circumference of releiving wrinkle and improvement skin dimness are the key that eye product demands.However, current country's alienation
Cosmetic market is inadequate to the attention of eyeware products and solution for lenses and contact lenses, and compared with facial-care product, eyeware products and solution for lenses and contact lenses is fewer.In addition it passes
The eyeware products and solution for lenses and contact lenses of system has only played basic replenishing water and preserving moisture, fragrant atmosphere effect, cannot play effect for eye part skin characteristic,
Cause the ability for solving the problems, such as eye skin limited.
Summary of the invention
The object of the present invention is to provide a kind of eye creams releived eye circumference wrinkle and improve skin dimness.
In order to solve the above-mentioned technical problem, the invention is realized by the following technical scheme:
According to an aspect of the present invention, a kind of edelweiss (LEONTOPODIUM ALPINUM) callus extract is provided
Preparation method, comprising the following steps:
(1), the flower for selecting edelweiss first uses 75% ethanol disinfection 30s in aseptic operating platform, then uses 0.1%HgCl2
Solution disinfection 5-10min, then with aseptic water washing 5-8 times, be placed on aseptic filter paper and dry；
(2), it aseptically by edelweiss flower shears at segment, is inoculated on callus inducing medium and carries out Fiber differentiation
Obtain primary culture edelweiss callus；
(3), by obtained primary culture edelweiss callus be forwarded to again edelweiss callus inducing medium carry out after
It is commissioned to train and supports twice, the edelweiss callus after being proliferated；
(4), 1:(1-3 in mass ratio) edelweiss callus and glycerol are mixed evenly, existed using high pressure homogenizer
Under the conditions of 25000PSI carry out broken wall homogenized to get.
In wherein some embodiments, induced medium selects MS for minimal medium in the step A, adds fine jade respectively
Rouge 3.5g/L, sucrose 30g/L, the basic element of cell division TDZ(Thidiazuron) 1mg/L, auxin 2,4-D(2,4- dichlorphenoxyacetic acid)
1.5mg/L。
In wherein some embodiments, the condition of culture of Fiber differentiation and subculture in the step A are as follows: cultivation temperature (25
± 1) DEG C, humidity 60-70%, first dark culture two weeks, then optical culture 25-35 days, optical culture press 16h under intensity of illumination 1000lx,
8h is alternately under dark condition.
According to another aspect of the present invention, a kind of eye cream is provided, the eye cream contains silk tree (ALBIZIA
JULIBRISSIN) bark extract and edelweiss (LEONTOPODIUM ALPINUM) callus extract.
In wherein some embodiments, silk tree (ALBIZIA JULIBRISSIN) bark is extracted in eye cream of the present invention
The mass percentage summation of object and edelweiss (LEONTOPODIUM ALPINUM) callus extract is 0.1-
10%。
In wherein some embodiments, silk tree (ALBIZIA JULIBRISSIN) bark extract and high mountain tinder
The mass ratio of careless (LEONTOPODIUM ALPINUM) callus extract is (1-10): (1-10).
In the preferred embodiment of the present invention, the eye cream can also contain 1 ‰ aqueous solutions of acetyl group tetrapeptide -5, second
The oily unsaponifiable matter of 1 ‰ aqueous solutions, olive (OLEA EUROPAEA) of acyl group hexapeptide -8.
In wherein some embodiments, the mass percentage of 1 ‰ aqueous solutions of the acetyl group tetrapeptide -5 is 0.1-
5%, the mass percentage of 1 ‰ aqueous solutions of the Argireline is 0.1-5%, the olive (OLEA
EUROPAEA) mass percentage of oily unsaponifiable matter is 0.1-5%.
The embodiment of the invention also provides a kind of eye cream, the eye cream contains the component of following mass percentage: sweet
Oily 1-10%, panthenol 0.1-3%, Sodium Hyaluronate 0.01-0.1%, carbomer 0.05-0.5%, sodium acrylate/acryloyldimethyl
Taurate Copolymer 0.1-1%, butanediol 1-5%, isononyl isononanoate 0.5-5%, caprylic/capric triglyceride 0.5-5%, benzene
The poly- trimethicone 0.5-5% of base, cetearyl glucoside 0.2-2%, cetostearyl alcohol 0.5-5%, olive (OLEA
EUROPAEA) oily unsaponifiable matter 0.1-5%, tocopherol (vitamin E) 0.1-1%, Butyrospermum parkii (BUTYROSPERMUM
PARKII) fruit fatty oil 0.1-5%, entire leaf Queensland nut (MACADAMIA INTEGRIFOLIA) seed oil 0.1-5%, acetyl group four
1 ‰ aqueous solution 0.1-5% of peptide -5,1 ‰ aqueous solution 0.1-5% of Argireline, yeast tunning extract 0.1-5%,
Silk tree (ALBIZIA JULIBRISSIN) bark extract 0.1-5%, edelweiss (LEONTOPODIUM ALPINUM) are more
Injured tissue extract 0.1-5%, phytosterol 0.1-1%, hexylene glycol 0.1-1%, parahydroxyacet-ophenone 0.3-0.5%, arginine
0.05-0.5%, water surplus.
Technical solution provided in an embodiment of the present invention has the benefit that
(1) a kind of edelweiss (LEONTOPODIUM ALPINUM) callus extract has been made in the embodiment of the present invention,
With good oxidation resistance, the synthesis of skin collagen and hyaluronic acid can be promoted, improve skin water humidity, promote skin
Skin elasticity, in addition can also inhibit tyrosinase activity, play the role of bright color.
(2) a kind of clear and rich soft and smooth eye cream of quality has been made in the embodiment of the present invention, is added to various plants and skims the cream off milk and matches
Active peptide components are closed, eye circumference skin moisture and nutrition is replenished in time, eye circumference of releiving in time wrinkle and to improve skin dull, dry and astringent etc.
Skin quality is moistened and is nourished, and eye circumference skin maintenance effect is promoted.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, embodiment of the present invention will be made into one below
Step ground detailed description, embodiments of the present invention are not limited thereto, for not specifically specified technological parameter or condition, can refer to
Routine techniques carries out.
Silk tree (ALBIZIA JULIBRISSIN) bark extract of the present invention is closed from pulse family albizzia plant
Joyous bark, which extracts, to be made, and it is solvent which, which can be with water, is extracted 1-3 time, combined extract, filter to get.Specifically
, it can be according to following technique: by the drying of silk tree bark, crushing；Then the water of 20 times of parts by weight is added, stirs lower immersion 2-3
A hour, filtering obtain first time filtrate；Filter residue is added to the water of 20 times of parts by weight, is stirred 2-3 hour of lower immersion, filtering,
Obtain second of filtrate；Will twice filtrate merge, centrifugation, obtain supernatant to get.
Embodiment 1
A kind of preparation method of edelweiss (LEONTOPODIUM ALPINUM) callus extract, including following step
It is rapid:
(1), the flower for selecting edelweiss first uses 75% ethanol disinfection 30s in aseptic operating platform, then uses 0.1%HgCl2
Solution disinfection 5min, then with aseptic water washing 8 times, be placed on aseptic filter paper and dry；Aseptically by edelweiss flower shears at
Segment is inoculated into progress Fiber differentiation on callus inducing medium and obtains primary culture edelweiss callus, wherein institute
The induced medium stated selects MS for minimal medium, adds agar 3.5g/L, sucrose 30g/L, basic element of cell division TDZ respectively
(Thidiazuron) 1mg/L, auxin 2,4-D(2,4- dichlorphenoxyacetic acid) 1.5mg/L；The primary culture edelweiss callus that will be obtained
Tissue is forwarded to edelweiss callus inducing medium again and carries out squamous subculture twice, the edelweiss callus group after being proliferated
It knits；The wherein condition of culture of Fiber differentiation and subculture are as follows: cultivation temperature (25 ± 1) DEG C, humidity 60-70%, first dark culture two weeks,
Optical culture 25 days again, optical culture press under intensity of illumination 1000lx under 16h, dark condition 8h alternately.
(2), edelweiss callus and glycerol are mixed evenly 1:3 in mass ratio, are existed using high pressure homogenizer
Under the conditions of 25000PSI carry out broken wall homogenized to get.
Embodiment 2
A kind of preparation method of edelweiss (LEONTOPODIUM ALPINUM) callus extract, including following step
It is rapid:
(1), the flower for selecting edelweiss first uses 75% ethanol disinfection 30s in aseptic operating platform, then uses 0.1%HgCl2
Solution disinfection 10min, then with aseptic water washing 5 times, be placed on aseptic filter paper and dry；Aseptically by edelweiss flower shears at
Segment is inoculated into progress Fiber differentiation on callus inducing medium and obtains primary culture edelweiss callus, wherein institute
The induced medium stated selects MS for minimal medium, adds agar 3.5g/L, sucrose 30g/L, basic element of cell division TDZ respectively
(Thidiazuron) 1mg/L, auxin 2,4-D(2,4- dichlorphenoxyacetic acid) 1.5mg/L；The primary culture edelweiss callus that will be obtained
Tissue is forwarded to edelweiss callus inducing medium again and carries out squamous subculture twice, the edelweiss callus group after being proliferated
It knits；The wherein condition of culture of Fiber differentiation and subculture are as follows: cultivation temperature (25 ± 1) DEG C, humidity 60-70%, first dark culture two weeks,
Optical culture 35 days again, optical culture press under intensity of illumination 1000lx under 16h, dark condition 8h alternately.
(2), edelweiss callus and glycerol are mixed evenly 1:1 in mass ratio, are existed using high pressure homogenizer
Under the conditions of 25000PSI carry out broken wall homogenized to get.
Embodiment 3
A kind of eye cream, the eye cream contain the component of following mass percentage: glycerol 1%, panthenol 3%, Sodium Hyaluronate
0.01%, carbomer 0.5%, yeast tunning extract 0.1%, sodium acrylate/sodium acryloyldimethyl taurate copolymers
0.1%, butanediol 5%, isononyl isononanoate 0.5%, caprylic/capric triglyceride 5%, phenyl trimethicone 0.5%, whale
Wax stearyl glucoside 2%, cetostearyl alcohol 0.5%, tocopherol (vitamin E) 1%, Butyrospermum parkii (BUTYROSPERMUM
PARKII) fruit fatty oil 5%, entire leaf Queensland nut (MACADAMIA INTEGRIFOLIA) seed oil 0.1%, phytosterol 1%,
Hexylene glycol 0.1%, parahydroxyacet-ophenone 0.5%, arginine 0.5%, silk tree (ALBIZIA JULIBRISSIN) bark extract
0.1%, edelweiss obtained by embodiment 1 (LEONTOPODIUM ALPINUM) callus extract 5%, water surplus.
Embodiment 4
A kind of eye cream, the eye cream contain the component of following mass percentage: glycerol 10%, panthenol 0.1%, Sodium Hyaluronate
0.1%, carbomer 0.05%, yeast tunning extract 5%, sodium acrylate/sodium acryloyldimethyl taurate copolymers 1%,
Butanediol 1%, isononyl isononanoate 5%, caprylic/capric triglyceride 0.5%, phenyl trimethicone 5%, cetearyl
Glucoside 0.2%, cetostearyl alcohol 5%, tocopherol (vitamin E) 0.1%, Butyrospermum parkii (BUTYROSPERMUM PARKII) fruit
Fatty oil 0.1%, entire leaf Queensland nut (MACADAMIA INTEGRIFOLIA) seed oil 5%, phytosterol 0.1%, hexylene glycol
1%, parahydroxyacet-ophenone 0.3%, arginine 0.05%, silk tree (ALBIZIA JULIBRISSIN) bark extract 5%, embodiment 2
Obtained edelweiss (LEONTOPODIUM ALPINUM) callus extract 0.1%, water surplus.
Embodiment 5
A kind of eye cream, the eye cream contain the component of following mass percentage: glycerol 5%, panthenol 1.5%, Sodium Hyaluronate
0.05%, carbomer 0.25%, yeast tunning extract 2.5%, sodium acrylate/sodium acryloyldimethyl taurate copolymers
0.5%, butanediol 2.5%, isononyl isononanoate 2.5%, caprylic/capric triglyceride 2.5%, phenyl trimethicone
2.5%, cetearyl glucoside 1%, cetostearyl alcohol 2.5%, tocopherol (vitamin E) 0.5%, Butyrospermum parkii
(BUTYROSPERMUM PARKII) fruit fatty oil 2.5%, entire leaf Queensland nut (MACADAMIA INTEGRIFOLIA) seed oil
2.5%, phytosterol 0.5%, hexylene glycol 0.5%, parahydroxyacet-ophenone 0.4%, arginine 0.25%, silk tree (ALBIZIA
JULIBRISSIN) bark extract 2.5%, edelweiss (LEONTOPODIUM ALPINUM) obtained by embodiment 1 be more
1 ‰ aqueous solutions of the oily unsaponifiable matter 0.1% of injured tissue extract 2.5%, olive (OLEA EUROPAEA), acetyl group tetrapeptide -5
5%, 1 ‰ aqueous solutions 0.1%, the water surplus of Argireline.
Embodiment 6
A kind of eye cream, the eye cream contain the component of following mass percentage: glycerol 5%, panthenol 1.5%, Sodium Hyaluronate
0.05%, carbomer 0.25%, yeast tunning extract 2.5%, sodium acrylate/sodium acryloyldimethyl taurate copolymers
0.5%, butanediol 2.5%, isononyl isononanoate 2.5%, caprylic/capric triglyceride 2.5%, phenyl trimethicone
2.5%, cetearyl glucoside 1%, cetostearyl alcohol 2.5%, tocopherol (vitamin E) 0.5%, Butyrospermum parkii
(BUTYROSPERMUM PARKII) fruit fatty oil 2.5%, entire leaf Queensland nut (MACADAMIA INTEGRIFOLIA) seed oil
2.5%, phytosterol 0.5%, hexylene glycol 0.5%, parahydroxyacet-ophenone 0.4%, arginine 0.25%, silk tree (ALBIZIA
JULIBRISSIN) bark extract 2.5%, edelweiss (LEONTOPODIUM ALPINUM) obtained by embodiment 2 be more
1 ‰ aqueous solutions of the oily unsaponifiable matter 5% of injured tissue extract 2.5%, olive (OLEA EUROPAEA), acetyl group tetrapeptide -5
0.1%, 1 ‰ aqueous solutions 5%, the water surplus of Argireline.
Embodiment 7
A kind of eye cream, the eye cream contain the component of following mass percentage: glycerol 5%, panthenol 1.5%, Sodium Hyaluronate
0.05%, carbomer 0.25%, yeast tunning extract 2.5%, sodium acrylate/sodium acryloyldimethyl taurate copolymers
0.5%, butanediol 2.5%, isononyl isononanoate 2.5%, caprylic/capric triglyceride 2.5%, phenyl trimethicone
2.5%, cetearyl glucoside 1%, cetostearyl alcohol 2.5%, tocopherol (vitamin E) 0.5%, Butyrospermum parkii
(BUTYROSPERMUM PARKII) fruit fatty oil 2.5%, entire leaf Queensland nut (MACADAMIA INTEGRIFOLIA) seed oil
2.5%, phytosterol 0.5%, hexylene glycol 0.5%, parahydroxyacet-ophenone 0.4%, arginine 0.25%, silk tree (ALBIZIA
JULIBRISSIN) bark extract 2.5%, edelweiss (LEONTOPODIUM ALPINUM) obtained by embodiment 2 be more
1 ‰ aqueous solutions of the oily unsaponifiable matter 2.5% of injured tissue extract 2.5%, olive (OLEA EUROPAEA), acetyl group tetrapeptide -5
2.5%, 1 ‰ aqueous solutions 2.5%, the water surplus of Argireline.
Embodiment 8 removes free radical test
Edelweiss obtained by embodiment 1-2 (LEONTOPODIUM ALPINUM) callus extract is surveyed
Examination, and organizes 1 with edelweiss extract as a comparison, wherein contrast groups 1 the preparation method comprises the following steps: by edelweiss drying,
It crushes；Then the water of 10 times of parts by weight is added, stirs 2-3 hour of lower immersion, filtering obtains filtrate；Centrifugation obtains supernatant,
To obtain the final product.
The specific test method is as follows:
Compound concentration is 1 × 10-2The DPPH ethanol solution of mol/L.By 80% second of embodiment 1-2 and contrast groups 1
Alcohol is configured to the testing sample solution that concentration is respectively 0.05,0.10,0.15,0.20,0.25mg/mL.Take 5mL sample molten respectively
Liquid and DPPH ethanol solution, and be uniformly mixed, avoid light place 30min, with the zeroing of 80% ethyl alcohol, measured at 517nm
Absorbance A i；Same method takes 5mL80% alcohol solvent and sample solution to be mutually mixed uniformly, avoid light place 30min respectively
Absorbance A j is measured afterwards；5mL80% alcohol solvent adds 5mLDPPH ethanol solution to mix, avoid light place 30min, measurement
Absorbance A c.Finally calculated according to following formula.
Free radical scavenging activity: IP%=[1- (Ai-Aj)/Ac] × 100, Ai is that sample and DPPH dehydrated alcohol are molten in formula
Absorption values after liquid effect；Aj is contribution of the sample itself to absorbance；Ac is DPPH ethanol solution itself
It absorbs.The ability measurement result for removing DPPH dehydrated alcohol see the table below 1.
1 free radical scavenging activity record sheet of table
From the data in table 1, it can be seen that edelweiss obtained by embodiment 1-2 (LEONTOPODIUM ALPINUM) callus mentions
Take object better than the oxidation resistance of edelweiss extract made from common process.
9 inhibitory activity against tyrosinase of embodiment
Test method: with the phosphate buffer (PBS) of 0.2mol/L pH=6.8 for solvent, the height of embodiment 1-2 is prepared respectively
The concentration of mountain edelweiss (LEONTOPODIUM ALPINUM) callus extract and the edelweiss extract of contrast groups 1
For the solution of 0.2mg/mL.Take the edelweiss (LEONTOPODIUM ALPINUM) of 0.9mL above-described embodiment 1-2 respectively more
The solution for the concentration 0.2mg/mL that injured tissue extract and the edelweiss extract of contrast groups 1 are prepared, is added 1mL L- junket
The phosphate buffer (PBS) of propylhomoserin (0.3mol) and 1mL pH=6.8 is preheated 10 minutes in 37 DEG C of thermostatic water baths, is added
0.1ml tyrosinase aqueous solution (1.0mg/ml, mcllvains buffer), is sufficiently stirred, and after 37 DEG C are reacted 15 minutes, surveys
Determine 475nm absorbance (D1).In addition, replace above-described embodiment 1-3's and comparative example 1-2 there is whitening with above-mentioned phosphate buffer
The concentration that active lime tree flower extract is prepared is that the solution of 0 .2mg/mL is similarly reacted, and measures absorbance (D2).For
Control, under conditions of sample solution and tyrosinase is not added, measures absorbance (D3).Tyrosine enzyme activity is calculated with following formula
Property inhibiting rate.
Inhibitory activity against tyrosinase (%)=(D2-D1)/(D2-D3) × 100
The results are shown in Table 2:
Table 2: inhibitory activity against tyrosinase test result table
Conclusion (of pressure testing): experiments have shown that, edelweiss (LEONTOPODIUM ALPINUM) callus of embodiment 1-2 extracts
Object has good tyrosinase activity inhibitory effect.
10 VISIA image analysis wrinkle of embodiment, the test of texture score value
The assessment of volunteer's effect is carried out to eye cream obtained by embodiment 3-7.The specific test method is as follows: (1) experimental subjects: choosing
Take totally 50 Symptoms be that eye part skin is loose, tested volunteer wrinkly, be all women, it is the oldest 50 years old, minimum
35 years old, it is divided into 5 groups at random, every group of 10 people.(2) sample: sample obtained by embodiment 3-7.(3) application method: daily in
Skin is loose, wrinkle position applies given the test agent, and once in the morning and once at night, each usage amount is 0.1g/10cm2.Using being counted after 30 days
Using effect.(4) effect is tested and assessed: taken pictures using VISIA face-image analysis system to volunteer's face (front, left side
45 °, 45 ° of right side direction), it is analyzed for wrinkle, texture two score values, score value is lower, and wrinkle of skin, texture are more unknown
It is aobvious.
Change rate %=(VISIA score valueAfter use- VISIA score valueBefore use)/VISIA score valueBefore use*100%
3 face-image of table analyzes wrinkle, texture score value change rate record
Seen from table 3, heretofore described embodiment 3-7, volunteer 4 weeks after use, wrinkle, texture score value change rate were equal
It is improved.Product has good anti-wrinkle effect.
The above described is only a preferred embodiment of the present invention, limitation in any form not is done to the present invention, therefore
All contents without departing from technical solution of the present invention, it is made to the above embodiment according to the technical essence of the invention any simply to repair
Change, equivalent variations and modification, all of which are still within the scope of the technical scheme of the invention.
Claims (9)
1. a kind of preparation method of edelweiss (LEONTOPODIUM ALPINUM) callus extract, feature exist
In, comprising the following steps:
(1), the flower for selecting edelweiss first uses 75% ethanol disinfection 30s in aseptic operating platform, then uses 0.1%HgCl2
Solution disinfection 5-10min, then with aseptic water washing 5-8 times, be placed on aseptic filter paper and dry；
(2), it aseptically by edelweiss flower shears at segment, is inoculated on callus inducing medium and carries out Fiber differentiation
Obtain primary culture edelweiss callus；
(3), by obtained primary culture edelweiss callus be forwarded to again edelweiss callus inducing medium carry out after
It is commissioned to train and supports twice, the edelweiss callus after being proliferated；
(4), 1:(1-3 in mass ratio) edelweiss callus and glycerol are mixed evenly, existed using high pressure homogenizer
Under the conditions of 25000PSI carry out broken wall homogenized to get.
2. the preparation of edelweiss (LEONTOPODIUM ALPINUM) callus extract according to claim 1
Method, which is characterized in that induced medium selects MS for minimal medium in the step A, adds agar 3.5g/L, sugarcane respectively
Sugared 30g/L, the basic element of cell division TDZ(Thidiazuron) 1mg/L, auxin 2,4-D(2,4- dichlorphenoxyacetic acid) 1.5mg/L.
3. the preparation of edelweiss (LEONTOPODIUM ALPINUM) callus extract according to claim 1
Method, which is characterized in that the condition of culture of Fiber differentiation and subculture in the step A are as follows: cultivation temperature (25 ± 1) DEG C, humidity
60-70%, first dark culture two weeks, then optical culture 25-35 days, optical culture presses 8h friendship under 16h, dark condition under intensity of illumination 1000lx
For progress.
4. a kind of eye cream, which is characterized in that the eye cream contains the described in any item edelweiss of claim 1-3
(LEONTOPODIUM ALPINUM) callus extract and silk tree (ALBIZIA JULIBRISSIN) bark extract.
5. eye cream according to claim 4, which is characterized in that silk tree (ALBIZIA JULIBRISSIN) in the eye cream
The mass percentage summation of bark extract and edelweiss (LEONTOPODIUM ALPINUM) callus extract
For 0.1-10%.
6. eye cream according to claim 4, which is characterized in that silk tree (ALBIZIA JULIBRISSIN) bark mentions
Taking the mass ratio of object and edelweiss (LEONTOPODIUM ALPINUM) callus extract is (1-10): (1-10).
7. eye cream according to claim 4, which is characterized in that the eye cream also 1 ‰ water containing acetyl group tetrapeptide -5
The oily unsaponifiable matter of solution, 1 ‰ aqueous solutions of Argireline, olive (OLEA EUROPAEA).
8. eye cream according to claim 7, which is characterized in that the quality of 1 ‰ aqueous solutions of the acetyl group tetrapeptide -5
Percentage composition is 0.1-5%, the mass percentage of 1 ‰ aqueous solutions of the Argireline is 0.1-5%, the oil
The mass percentage of olive (OLEA EUROPAEA) oil unsaponifiable matter is 0.1-5%.
9. a kind of eye cream, which is characterized in that the eye cream contains the component of following mass percentage: glycerol 1-10%, panthenol
0.1-3%, Sodium Hyaluronate 0.01-0.1%, carbomer 0.05-0.5%, sodium acrylate/sodium acryloyldimethyl taurate copolymerization
Object 0.1-1%, butanediol 1-5%, isononyl isononanoate 0.5-5%, caprylic/capric triglyceride 0.5-5%, the poly- trimethyl silicane of phenyl
Oxygen alkane 0.5-5%, cetearyl glucoside 0.2-2%, cetostearyl alcohol 0.5-5%, olive (OLEA EUROPAEA) oil are not
Saponified 0.1-5%, tocopherol (vitamin E) 0.1-1%, Butyrospermum parkii (BUTYROSPERMUM PARKII) fruit fatty oil 0.1-
5%, entire leaf Queensland nut (MACADAMIA INTEGRIFOLIA) seed oil 0.1-5%, acetyl group tetrapeptide -5 1 ‰ aqueous solutions
0.1-5%, 1 ‰ aqueous solution 0.1-5% of Argireline, yeast tunning extract 0.1-5%, silk tree (ALBIZIA
JULIBRISSIN) described in any item edelweiss (the LEONTOPODIUM of bark extract 0.1-5%, claim 1-3
ALPINUM) callus extract 0.1-5%, phytosterol 0.1-1%, hexylene glycol 0.1-1%, parahydroxyacet-ophenone 0.3-
0.5%, arginine 0.05-0.5%, water surplus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910518203.2A CN110123700A (en) | 2019-06-14 | 2019-06-14 | A kind of eye cream releived eye circumference wrinkle and improve skin dimness |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910518203.2A CN110123700A (en) | 2019-06-14 | 2019-06-14 | A kind of eye cream releived eye circumference wrinkle and improve skin dimness |
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| CN110123700A true CN110123700A (en) | 2019-08-16 |
Family
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|---|---|---|---|
| CN201910518203.2A Pending CN110123700A (en) | 2019-06-14 | 2019-06-14 | A kind of eye cream releived eye circumference wrinkle and improve skin dimness |
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| CN (1) | CN110123700A (en) |
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Application publication date: 20190816 |