CN109770362A - A kind of relieving alcoholism and protecting liver health care product and preparation method thereof - Google Patents
A kind of relieving alcoholism and protecting liver health care product and preparation method thereof Download PDFInfo
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- CN109770362A CN109770362A CN201910198210.9A CN201910198210A CN109770362A CN 109770362 A CN109770362 A CN 109770362A CN 201910198210 A CN201910198210 A CN 201910198210A CN 109770362 A CN109770362 A CN 109770362A
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Abstract
The invention discloses a kind of relieving alcoholism and protecting liver health care products and preparation method thereof.Expected based on the acetic acid fungus extract containing high activity alcohol dehydrogenase, acetaldehyde dehydrogenase and metabolite acetic acid fungin, granule, tablet or capsule is made.This product has good relieving alcoholism and preventing drunkenness effect, after alcohol user takes this product before drinking, can make alcohol in gastrointestinal tract rapid metabolization acetic acid to be absorbed or drain, reduce injury of the excessive alcohol to human body, relieving alcoholism and protecting liver.This product is naturally conveniently, safely eaten, and is easy to save carrying, has biggish industrial production value.
Description
Technical field
The present invention relates to disintoxicating products, and in particular to a kind of relieving alcoholism and protecting liver health care product and preparation method thereof.
Background technique
Long-term heavy drinking will lead to various acute and chronic hepatic diseases.Studies have shown that after ethyl alcohol enters liver cell, warp
Cross alcohol dehydrogenase, three approach of hydrogen peroxide body catabolic enzyme and hepatomicrosome oxidation of ethanol enzyme system are oxidized to acetaldehyde.It is excessive
Drinking causes a large amount of acetaldehyde to be deposited in liver, generates apparent toxic effect to liver cell, directly or indirectly leads to hepatic injury.It is long
Phase heavy drinking, excessive drinking not only result in alcoholism, also result in alcoholic liver disease, including: alcoholic fatty liver, wine
Essence hepatitis, alcoholic fibrosis, alcoholic cirrhosis and mild alcoholic liver disease.Long-term intake alcohol induces hepatic injury etc.
Series of problems has become one of most important public health problem in the whole world, by the concern of academia, but so far still without treatment
The specific medicament of alcoholic liver disease (ALD).And to pass through the conversion of liver into most of intracorporal drug, if sufferer liver function
Decline, drug enter in vivo, certainly will increase burden of liver.Therefore, natural microbial function food is developed to liver function
The protection that can be damaged has important practical significance.
" relieving the effect of alcohol " product currently on the market is broadly divided into chemicals class, enzyme preparation class, Chinese materia medica preparation class.It is wherein chemical
Preparation class is mostly the ingredients such as excitant, vitamin and amino acid, there is certain sobering-up functions, but the organ injury caused by alcohol and
Slow poisoning has little effect；Enzyme preparation class mainly contains acetaldehyde dehydrogenase, but price is costly；Chinese materia medica preparation class a great variety,
But effect is little.Through extensive market survey and crowd's probationary certificate, the effect of these products is undesirable.
Acetic acid bacteria be it is a kind of can general name by alcohol oxidation at the microorganism of acetic acid, be the weight in vinegar and fruit vinegar production
Want microorganism.Acetic acid bacteria somatic cells matter and cell membrane contain complicated alcohol dehydrogenase and acetaldehyde-dehydrogenase enzyme system.In addition, excellent
Strain Acetobacter xylinum has good alcohol metabolism ability, stronger antioxidant activity, and fermentation period is short, Industrialized processing technique
It is more mature.Meanwhile people in recent years increasingly deepen the idea of health care, therefore for the functionality with health ability
The exploitation of microniological proudcts, has much market potential.Therefore according to the physiology of alcoholic liver injury, Biochemical Characteristics, using acetic acid bacteria
Functional characteristic opens promotion liver function health care and Related product for alcoholic liver injury invention acetic acid bacteria relieving alcoholism and protecting liver product
Hair has great importance.
Acetic acid bacteria quasi-microorganism mainly includes acetobacter (Acetobaeter) and gluconobacter suboxydans category
Gluconobaceter microorganism), because the two can generate acetic acid with Oxidation of Alcohol, also referred to as acetic acid bacteria.System
The industrial most common production acetobacter of vinegar is line film acetic acid bacteria (Acetobaete aceti) and stench acetobacter
(Acetobaete rancence), gluconobacter suboxydans microorganism belonging to genus Oxidation of Alcohol are weaker than acetobacter for the ability of acetic acid, but
Relative to other microorganisms, the ability for generating alcohol dehydrogenase and acetaldehyde dehydrogenase is also relatively strong, also is suitable as both enzymes
Extract raw material.
Acetic acid bacteria is functional food, modern microbiology, biochemistry, pathology etc. to the research of liver dysfunction
The height of equal subjects combines, at present it has been reported that relieving alcoholism and protecting liver product relevant to acetic acid bacteria mainly with functional raw material, example
Such as Chinese herbal medicine, fragrance, honey, cereal seed are substrate, by acetic acid bacteria, the mix bacterium agent containing acetic acid bacteria or straight
It connects using dehydrogenation enzyme fermentation and is made, but the component and preparation process complexity of product is made, and product presence is made and relieves the effect of alcohol slowly, and
The problems such as aggravating liver burden.
Acetic acid fungin (Bacterial Cellulose, BC) is the gel mould generated by the bacteriums heterotrophism such as acetobacter
Shape object.Compared with plant cellulose and tunicin, it is the cellulose of a kind of " super clean ", and undope other polysaccharide, tool
There are many good characteristics, have good development prospect in functional food production field.But there is not yet utilizing acetic acid bacteria fibre
Dimension element improves the relieving the effect of alcohol of acetic acid bacteria or its enzyme preparation, the report of protect liver effect, main reason is that: prior art is generally being fermented
After carry out filter progress, cause acetic acid fungin by separation remove.
Summary of the invention
For the deficiency for solving existing disintoxicating product, the present invention provides a kind of relieving alcoholism and protecting liver health care products and preparation method thereof.
In order to achieve the above objectives, the invention adopts the following technical scheme:
A kind of relieving alcoholism and protecting liver health care product, the health care product include major ingredient, and the major ingredient includes component A and component B, component A choosing
From one of acetic acid bacteria quasi-microorganism thallus, acetic acid bacteria quasi-microorganism cellular lysate object, alcohol dehydrogenase, acetaldehyde dehydrogenase or
A variety of, component B is acetic acid fungin (BC).
Preferably, the major ingredient is by physical measure by acetic acid bacteria quasi-microorganism (for example, Dichlorodiphenyl Acetate mushroom microorganism sends out
Zymotic fluid homogenate) carry out cellular lysate (lysate contains alcohol dehydrogenase, acetaldehyde dehydrogenase), and with the acetic acid bacteria quasi-microorganism
After the mixing of metabolite acetic acid fungin, freeze-dried and manufactured acetic acid fungus extract (freeze-dried powder) contains in major ingredient
Some alcohol dehydrogenases, acetaldehyde dehydrogenase are mainly derived from acetic acid bacteria quasi-microorganism, in order to control the content standard of enzyme, can adopt
Use a small amount of commercialization alcohol dehydrogenase, acetaldehyde-dehydrogenase enzyme preparation as supplementing.
Preferably, the content standard of the alcohol dehydrogenase is >=1.2U/mg, the content standard of acetaldehyde dehydrogenase is >=
1.2U/mg, the content standard of acetic acid fungin are >=0.30mg/mg.
Preferably, the acetic acid bacteria quasi-microorganism is selected from wood gluconacetobacter, in fermentation stage, cell membrane and is metabolized
There are the alcohol dehydrogenase of high activity and acetaldehyde dehydrogenases on acetic acid product fungin.
Preferably, the major ingredient further includes enzyme stabilizers, and enzyme stabilizers are selected from Mg2+、Fe2+Or Fe3+, freeze-dried
Before be added (i.e. after homogenate), the dosages of enzyme stabilizers according to final concentration of 0.1 in the system obtained by homogenate (lysate)~
10mM is calculated.
Preferably, the health care product further includes auxiliary material, and the mass ratio of auxiliary material and major ingredient is 0.1~10:1, and auxiliary material is selected from hard
One or both of fatty acid magnesium, cornstarch.
Preferably, the health care product further include have hepatoprotective effect natural origin (for example, Quercetin, dried orange peel, chrysanthemum,
The extract etc. of flower of Radix Puerariae) or synthesis active material, the mass ratio of active material and major ingredient (acetic acid fungus extract) is 1:0.5
~3.
A kind of preparation method of relieving alcoholism and protecting liver health care product, comprising the following steps:
Above-mentioned acetic acid bacteria quasi-microorganism progress fermented and cultured is obtained into thallus fermentation liquid, by thallus homogenate of fermentation broth, so that bacterium
Body cracking and the metabolite mixing generated with thallus in fermented and cultured, obtain containing alcohol dehydrogenase, acetaldehyde dehydrogenase and acetic acid
The lysate of fungin；Enzyme stabilizers are added into the lysate；Then it is freeze-dried, obtains freeze-dried powder (i.e. above-mentioned vinegar
Sour fungus extract).
Preferably, the fermented and cultured is specifically includes the following steps: by acetic acid bacteria quasi-microorganism (for example, the wooden glucose vinegar bar
Bacterium) it is inoculated in fluid nutrient medium, 28~32 DEG C of cultures, 150~180rpm of cycle of oscillation cultivates 70~72 hours, obtains thallus
Fermentation liquid.
Preferably, the homogenate is specifically includes the following steps: 8~10 times of volumes are added into the thallus fermentation liquid
Then the 0.05M phosphate buffer of pH=6~7 is homogenized 3~5 minutes in 25~30 DEG C, 8000~12000rpm, obtains lysate.
Preferably, the enzyme stabilizers are selected from Mg2+、Fe2+Or Fe3+, final concentration of 0.1~10mM in lysate.
Preferably, the condition of the freeze-drying includes: 6~8mm of cellular lysate liquid thickness, and -20 DEG C to -30 DEG C normal pressures are pre-
Freeze 8~10h, then under 10~100pa, -40 to -60 DEG C of dryings are for 24 hours.
Preferably, further comprising the steps of: granule, piece is made after the acetic acid fungus extract is mixed with above-mentioned auxiliary material
The dosage forms such as agent or capsule；Alternatively, by the acetic acid fungus extract and above-mentioned auxiliary material and natural origin with hepatoprotective effect or
The dosage forms such as granule, tablet or capsule are made after the active material mixing of synthesis.
Above-mentioned acetic acid fungus extract prepare relieving alcoholism and protecting liver health care product, the application in drug, which includes
Acetic acid fungin, alcohol dehydrogenase, acetaldehyde dehydrogenase.
The beneficial effects of the present invention are embodied in:
Liver-protecting and alcoholism-relieving health care product provided by the invention is mainly with acetic acid bacteria quasi-microorganism and its metabolite acetic acid bacteria fiber
Element is prepared as a raw material, and promotes alcohol dehydrogenase, acetaldehyde dehydrogenase contained by acetic acid bacteria quasi-microorganism using acetic acid fungin
Effect, effectively accelerate alcohol metabolism in stomach, reduce burden of liver, have the effect of significant relieving alcoholism and protecting liver and liver protection,
Liver protection effect, instant edible are easy to save carrying, have biggish market popularization value.
Further, the present invention is by adding certain active material (for example, Quercetin, dried orange peel, chrysanthemum, flower of Radix Puerariae
Extract etc.), improve liver function, increases the detoxication of liver.
Liver-protecting and alcoholism-relieving health care product provided by the invention, preparation process is simple, and raw material is easy to get, is safe, is at low cost, has larger
Industrial production value, and alcohol dehydrogenase, acetaldehyde dehydrogenase, acetic acid bacteria fiber in health product raw material (acetic acid fungus extract)
Plain reasonable mixture ratio of components, the effect of significant relieving alcoholism and protecting liver can be provided.
Specific embodiment
Technical solution of the present invention is described further below with reference to embodiment.Following embodiments are only used for explaining this
Invention, rather than limiting the scope of the invention.
(1) preparation of relieving alcoholism and protecting liver health care product
Mechanism provided by the invention of relieving the effect of alcohol uses the relieving the effect of alcohol as main active using alcohol dehydrogenase and acetaldehyde dehydrogenase
Formula, taking orally alcohol dehydrogenase containing high activity and acetaldehyde made of acetic acid bacteria thallus and its metabolin acetic acid fungin
The acetic acid fungus extract of dehydrogenase, so that ethyl alcohol is broken down into acetic acid in the gastrointestinal tract.For acetic acid bacteria quasi-microorganism,
Ethanol decomposition is acetic acid by first-pass metabolism by way of almost the same in the intracorporal metabolic pathway of people with ethyl alcohol by the generation of acetic acid,
And acetic acid, so ethyl alcohol absorption can be effectively reduced, achievees the effect that relieving alcoholism and preventing drunkenness to the toxic action very little of human body.
The preparation process of relieving alcoholism and protecting liver health care product is as follows: raw material (for example, being derived from fermentation liquid) acetic acid bacteria quasi-microorganism is thin
Cell space and acetic acid fungin carry out cellular lysate by high-speed homogenization and mix with acetic acid fungin；Enzyme stabilizers are added
Mg2+、Fe2+Or Fe3+, make its final concentration of 0.1~10mM；Then it is freeze-dried, freeze-dried powder is made to get white powder is arrived
Acetic acid fungus extract；Expected based on acetic acid fungus extract, pharmaceutical adjunct is added, tablet, capsule or granule is made.
Since the alcohol dehydrogenase and acetaldehyde dehydrogenase of more amount from acetic acid bacteria quasi-microorganism, can be obtained, therefore with vinegar
Sour mushroom microorganism is raw material.With the bacterial strain with high ethano dehydrogenase and aldehyde dehydrogenase activity, for example, being isolated from edible persimmon
Wood gluconacetobacter in sub- vinegar, is inoculated in fluid nutrient medium, and culture obtains thallus fermentation liquid.When culture is completed, fermentation liquid bacterium
Body quantity reaches 2 × 109CFUs/mL carries out determination of activity by ADH and ALDH kit, and alcohol dehydrogenase enzyme activity reaches
0.15~0.20U/mL, acetaldehyde-dehydrogenase enzyme activity reach 0.12~0.18U/mL.Meanwhile vinegar in visible fermentation liquid under scanning electron microscope
Sour fungin is extracellular products, and measuring acetic acid bacteria cellulose wet-coating weight is 25.36g/100mL, and acetic acid bacteria is fine after freeze-drying
Tieing up plain dry weight is 0.37g/100mL.
Cracking and blending process: phosphate buffer is added into thallus fermentation liquid, is homogenized in high speed homogenizer at room temperature.
Active material is wanted based on acetic acid fungus extract, is mixed with suitable auxiliary material (magnesium stearate, cornstarch etc.),
It is made into tablet, capsule, granule (i.e. health care product) etc. as required and takes that (in liquid preparation, alcohol dehydrogenase and acetaldehyde are de-
Hydrogen enzyme, which is easy to happen variation, leads to loss of activity).The Related Component with hepatoprotective effect can also be added as auxiliary active agent,
As Quercetin, dried orange peel, chrysanthemum, flower of Radix Puerariae extract (additional amount and the ratio of acetic acid fungus extract be 1:0.5~3).Wherein,
Quercetin shows stronger cytotoxicity to liver cancer cells, can effectively inhibit the growth of liver cancer cells；Have to hepatic injury
Good protective effect and the effect of certain anti-hepatic fibrosis have significant protect liver effect.
(2) the effect of relieving alcoholism and protecting liver health care product, tests
Anxious poison test display: acetic acid fungus extract is 30g/kg to the maximal tolerance dose of mouse.
Pharmacodynamic test result proves: acetic acid fungus extract shows stronger alcohol dehydrogenase and aldehyde dehydrogenase activity,
Mice drunk can effectively be prevented, extend the mice drunk time, reduce drunk rate, shorten and sober up the time.
Experiment is compared referring to alcohol dehydrogenase enzyme activity in acetic acid fungus extract: the result shows that, with exclusive use ethyl alcohol
Dehydrogenase enzyme preparation compares, and acetic acid fungin and alcohol dehydrogenase enzyme preparation, which is used in combination, can extend the mice drunk time, solves
Wine effect is more significant.
This health care product is worked, or so half an hour takes before drinking by taking orally through gastrointestinal tract.It is taken before drinking
This health care product can make alcohol be absorbed or drain in gastrointestinal tract rapid metabolization acetic acid, to reduce excessive alcohol to human body
Injury.
(3) formulation examples
Example 1
For preparing capsule 1000:
Wood gluconacetobacter (CGMCC 3917, bibliography: Dehui Lin, Patricia Lopez-Sanchez, Rui
Li,Zhixi Li*.Production of bacterialcellulose by Gluconacetobacterhansenii
CGMCC 3917using only waste beer yeast asnutrient source,Bioresource
Technology, 2014,151C:113-119.) be inoculated in fluid nutrient medium (culture medium composition: glucose 20g, yeast extract 5g,
Dipotassium hydrogen phosphate 1g, magnesium sulfate 15g, distilled water 1L, dehydrated alcohol 20mL), 30 DEG C of cultures, cycle of oscillation 150rpm, culture 72
After hour, the 0.05M phosphate buffer of 10 times of amount pH=6.5 is added, 12000rpm is homogenized 5 minutes in high speed homogenizer, is added
Enzyme stabilizers (such as ferric sulfate, final concentration 0.1mM) it is freeze-dried (freeze-drying condition: cellular lysate liquid thickness 6mm, -20 DEG C
Under pre-freeze 10h, 30Pa, -50 DEG C of dryings for 24 hours), obtain the acetic acid fungus extract dry powder of white powder.
Extract obtained dry powder is directly encapsulated, and every weighs about 0.03g, every dry powder 30mg (its of fungus extract containing acetic acid
Middle alcohol dehydrogenase and aldehyde dehydrogenase activity >=40U, the fungin 12mg containing acetic acid)
Usage and dosage: oral, 1~2 every time, 10~30 DEG C of warm water are taken before drinking.
Example 2
For preparing capsule 1000:
Wood gluconacetobacter in example 1 be inoculated in fluid nutrient medium (culture medium composition: glucose 20g, yeast extract 5g,
Dipotassium hydrogen phosphate 1g, magnesium sulfate 15g, distilled water 1L, dehydrated alcohol 20mL), 30 DEG C of cultures, cycle of oscillation 150rpm, culture 72
After hour, the 0.05M phosphate buffer of 10 times of amount pH=6.5 is added, 12000rpm is homogenized 5 minutes in high speed homogenizer, is added
Enzyme stabilizers (such as ferric sulfate, final concentration 0.1mM) it is freeze-dried (freeze-drying condition: cellular lysate liquid thickness 6mm, -20 DEG C
Under pre-freeze 10h, 30Pa, -50 DEG C of dryings for 24 hours), obtain the acetic acid fungus extract dry powder of white powder.
Extract obtained dry powder mixes well in the following proportions with auxiliary material:
Acetic acid fungus extract dry powder (major ingredient) 30g
Cornstarch (auxiliary material) 35g
Magnesium stearate (auxiliary material) 5g
Preparation is carried out by the technique of galenic pharmacy capsule, every weighs about 0.07g, the every dry powder of fungus extract containing acetic acid 30mg
(wherein alcohol dehydrogenase and aldehyde dehydrogenase activity >=40U, the fungin 12mg containing acetic acid)
Usage and dosage: oral, 1~2 every time, 10~30 DEG C of warm water are taken before drinking.
Example 3
For preparing capsule 1000:
Wood gluconacetobacter in example 1 be inoculated in fluid nutrient medium (culture medium composition: glucose 20g, yeast extract 5g,
Dipotassium hydrogen phosphate 1g, magnesium sulfate 15g, distilled water 1L, dehydrated alcohol 20mL), 30 DEG C of cultures, cycle of oscillation 150rpm, culture 72
After hour, the 0.05M phosphate buffer of 10 times of amount pH=6.5 is added, 12000rpm is homogenized 5 minutes in high speed homogenizer, is added
Enzyme stabilizers (such as ferric sulfate, final concentration 0.1mM) it is freeze-dried (freeze-drying condition: cellular lysate liquid thickness 6mm, -20 DEG C
Under pre-freeze 10h, 30Pa, -50 DEG C of dryings for 24 hours), obtain the acetic acid fungus extract dry powder of white powder.
Extract obtained dry powder and auxiliary material (cornstarch and magnesium stearate) and Quercetin mix well in the following proportions.
Preparation is carried out by the technique of galenic pharmacy capsule, every weighs about 0.07g, the every dry powder of fungus extract containing acetic acid 30mg
(wherein alcohol dehydrogenase and aldehyde dehydrogenase activity >=40U, the fungin 12mg containing acetic acid), 15mg containing Quercetin.
Usage and dosage: oral, 1~2 every time, 10~30 DEG C of warm water are taken before drinking.
Example 4
For preparing capsule 1000, raw materials used weight proportion is as follows:
Acetic acid fungus extract dry powder (with the same batch of example 1) 30g
Quercetin 20g
Cornstarch 20g
Preparation is carried out by the technique of galenic pharmacy capsule, every weighs about 0.07g, every dry powder of fungus extract containing acetic acid
30mg, 20mg containing Quercetin.
Usage and dosage: oral, 1~2 every time, 10~30 DEG C of warm water are taken before drinking.
Example 5
For preparing capsule 1000, raw materials used weight proportion is as follows:
Acetic acid fungus extract dry powder (with the same batch of example 1) 30g
Quercetin 50g
Cornstarch 220g
Preparation is carried out by the technique of galenic pharmacy capsule, every weighs about 0.30g, every dry powder of fungus extract containing acetic acid
30mg, 50mg containing Quercetin.
Usage and dosage: oral, 1~2 every time, 10~30 DEG C of warm water are taken before drinking.
In short, acetic acid bacteria somatic cells and its metabolite acetic acid fungin are passed through homogenate, freeze-drying by the present invention
And etc. relieving alcoholism and protecting liver health care product is made.This product improves the activity of alcohol dehydrogenase, acetaldehyde dehydrogenase, can effectively accelerate wine
Essence metabolism, improves liver function, increases the detoxication of liver, has effects that relieve the effect of alcohol, anti-intoxication, protect liver, and natural safety, square
Just it eats, is easy to save carrying, there is biggish industrialized production and market popularization value.
Claims (10)
1. a kind of relieving alcoholism and protecting liver health care product, it is characterised in that: the health care product includes major ingredient, and the major ingredient includes component A and component
B, component A are selected from acetic acid bacteria quasi-microorganism thallus, acetic acid bacteria quasi-microorganism cellular lysate object, alcohol dehydrogenase, acetaldehyde dehydrogenase
One of or it is a variety of, component B be acetic acid fungin.
2. a kind of relieving alcoholism and protecting liver health care product according to claim 1, it is characterised in that: the major ingredient is by the micro- life of acetic acid mushroom
Part or all of thallus in the cultivating system of object is cracked, and the metabolite acetic acid bacteria with the acetic acid bacteria quasi-microorganism
After cellulose mixing, freeze-dried and manufactured acetic acid fungus extract.
3. a kind of relieving alcoholism and protecting liver health care product according to claim 2, it is characterised in that: in the acetic acid fungus extract, ethyl alcohol
The content of dehydrogenase is >=1.2U/mg, and the content of acetaldehyde dehydrogenase is >=1.2U/mg, the content of acetic acid fungin is >=
0.30mg/mg。
4. a kind of relieving alcoholism and protecting liver health care product according to claim 1, it is characterised in that: the acetic acid bacteria quasi-microorganism is selected from wood
Gluconacetobacter.
5. a kind of relieving alcoholism and protecting liver health care product according to claim 1, it is characterised in that: the major ingredient further includes enzyme stabilizers,
Enzyme stabilizers are selected from Mg2+、Fe2+Or Fe3+。
6. a kind of relieving alcoholism and protecting liver health care product according to claim 1, it is characterised in that: the health care product further includes auxiliary material, or
Person, the health care product further include auxiliary material and the active material with hepatoprotective effect, and the mass ratio of auxiliary material and major ingredient is 0.1~10:
1, the mass ratio of active material and major ingredient is 1:0.5~3.
7. a kind of preparation method of relieving alcoholism and protecting liver health care product, it is characterised in that: the following steps are included:
Acetic acid bacteria quasi-microorganism progress fermented and cultured is obtained into thallus fermentation liquid, by thallus homogenate of fermentation broth, so that a part or complete
Portion's cellular lysate simultaneously in fermented and cultured thallus generate metabolite mix, obtain containing alcohol dehydrogenase, acetaldehyde dehydrogenase and
The lysate of acetic acid fungin；Enzyme stabilizers are added into the lysate；Then it is freeze-dried, obtains acetic acid bacteria extraction
Object.
8. a kind of preparation method of relieving alcoholism and protecting liver health care product according to claim 7, it is characterised in that: the specific packet of homogenate
Include following steps: in Xiang Suoshu thallus fermentation liquid be added 8~10 times of volumes phosphate buffer, then in 25~30 DEG C,
8000~12000rpm is homogenized 3~5 minutes, obtains lysate；The enzyme stabilizers are selected from Mg2+、Fe2+Or Fe3+, enzyme stabilizers exist
Final concentration of 0.1~10mM in lysate.
9. a kind of preparation method of relieving alcoholism and protecting liver health care product according to claim 7, it is characterised in that: mention the acetic acid bacteria
Granule, tablet or capsule are made after taking object to mix with auxiliary material；Alternatively, by the acetic acid fungus extract and auxiliary material and there is guarantor
Granule, tablet or capsule is made after the active material mixing of liver effect.
10. a kind of acetic acid fungus extract prepare relieving alcoholism and protecting liver health care product, the application in drug, it is characterised in that: the acetic acid bacteria
Extract includes acetic acid fungin.
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