CN109762775A - Compound rich strontium bacterial manure of one kind and preparation method thereof - Google Patents
Compound rich strontium bacterial manure of one kind and preparation method thereof Download PDFInfo
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- CN109762775A CN109762775A CN201910214037.7A CN201910214037A CN109762775A CN 109762775 A CN109762775 A CN 109762775A CN 201910214037 A CN201910214037 A CN 201910214037A CN 109762775 A CN109762775 A CN 109762775A
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- strontium
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Abstract
The invention discloses a kind of compound rich strontium bacterial manure and preparation method thereof, preparation methods: strain activation and culture base preparation；Actication of culture: original bacteria suspension is inoculated in strain activation and culture base obtained, culture obtains activated spawn suspension；Three kinds of rich strontium bacterium synchronous fermentations are carried out, rich strontium microbial inoculum is obtained；The rich strontium bacterial manure of rich strontium microbial inoculum is mixed in proportion.Rich strontium bacterial manure is produced using photosynthetic bacteria, lactic acid bacteria and saccharomycetes to make fermentation, both can avoid single bacterium richness strontium, inhibits the growth and breeding of effective viable bacteria since strontium additive amount is too high, also can avoid causing crops richness strontium insufficient since strontium additive amount is very little.Entire fermentation process save the cost, it is high to obtain bacterial manure rate；Three kinds of bacterium are fermented respectively simultaneously, can shorten fermentation period；Using three kinds of bacterium richness strontiums, single bacterium richness strontium can avoid, if the too high growth and breeding that can inhibit effective viable bacteria of strontium additive amount, also can avoid causing crops richness strontium insufficient since strontium additive amount is very little.
Description
Technical field
A kind of compound rich strontium bacterial manure of the invention and preparation method thereof, belongs to technical field of fertilizer production.
Background technique
Strontium element is microelement necessary to human body, very useful to human health.Growth and development of the strontium to bone, machine
The metabolism of body and maintenance human normal physiological function have positive facilitation.Strontium can be with preventing hypertension and painstaking effort
Pipe disease, it is related with longevity.Scarce strontium can cause a series of illnesss, such as internal metabolic disorder, and limbs are out of strength, cold perspiration, bone hair
Slow and osteoporosis etc. is educated, can also make one to suffer from tumour when serious.Therefore, rationally mending strontium is the big of a n-th-trem relation n fitness-for-all
Thing.Often edible rich strontium agricultural product are one of the optimal paths for mending strontium.
Inorganic strontium and base manure are mainly added into soil during cultivated crop for rich strontium agricultural product to promote at present
Into crops richness strontium.But strontium is added directly into soil certain requirement to soil quality, if such as salt-soda soil, strontium ion
Rich strontium not only may be not achieved to crops in this way and imitated with the carbonate or sulfate radical plasma formation strontium salt precipitating in soil
Fruit, instead can because excessive buildup to soil caused by heavy metal pollution.Therefore, inventing a kind of can not only promote crops richness strontium but also not
Cause the rich strontium fertilizer of soil pollution very crucial in application from now on.
Summary of the invention
The present invention provides a kind of compound rich strontium bacterial manure and preparation method thereof, and this method high production efficiency, environmental pollution are small,
It is high to obtain bacterial manure rate for save the cost；Three kinds of bacterium are fermented respectively simultaneously, can shorten fermentation period；Using three kinds of bacterium richness strontiums, can keep away
When exempting from single bacterium richness strontium, if strontium additive amount too the high growth and breeding that can inhibit effective viable bacteria the problem of, also can avoid adding due to strontium
Dosage is very little and causes crops richness strontium insufficient.
The invention is realized by the following technical scheme:
A kind of preparation method of compound rich strontium bacterial manure, comprising the following steps:
1) prepared by strain activation and culture base:
Photosynthetic bacteria activation medium, lactic acid bacteria activation medium and saccharomycete activation medium are prepared respectively；
2) actication of culture:
Original photosynthetic bacteria suspension is inoculated in photosynthetic bacteria activation medium made from step 1), illumination Anaerobic culturel obtains
The photosynthetic bacteria suspension of activation；
Picking is stored in the lactic acid bacteria single colonie access of MRS culture medium in the lactic acid bacteria activation medium made from step 1), detests
Oxygen constant-temperature shaking culture, the lactic acid bacteria suspension activated；
Picking is stored in saccharomycete activation medium made from the saccharomycete single colonie access step 1) of PDA culture medium, aerobic
Constant-temperature shaking culture, the yeast bacteria suspension activated；
3) preparation of rich strontium fermentation culture:
Photosynthetic bacteria richness strontium fermentating formula liquid, lactic acid bacteria richness strontium fermentating formula liquid and saccharomycete richness strontium fermentating formula are prepared respectively
Liquid；
4) rich strontium fermentation prepares rich strontium microbial inoculum:
Rich strontium fermentation, the photosynthetic bacteria, lactic acid bacteria and saccharomycete are carried out to the photosynthetic bacteria, lactic acid bacteria and saccharomycete respectively
The fermentation of rich strontium synchronous carry out:
The fermentation of photosynthetic bacteria richness strontium: the photosynthetic bacteria suspension of activation is inoculated in rich strontium Fermentation by Photosynthetic Bacteria culture medium, is added
Photosynthetic bacteria richness strontium fermentating formula liquid, the Anaerobic culturel under illumination condition obtain photosynthetic bacteria richness strontium microbial inoculum；
The fermentation of lactic acid bacteria richness strontium: by the lactic acid bacteria bacterial suspension inoculation of activation in rich strontium lactobacillus-fermented culture medium, lactic acid is added
Bacterium richness strontium richness strontium fermentating formula liquid, anaerobism constant-temperature shaking culture obtain lactic acid bacteria richness strontium microbial inoculum；
The fermentation of saccharomycete richness strontium: by the saccharomycete bacterial suspension inoculation of activation in rich strontium saccharomycetes to make fermentation culture medium, yeast is added
Bacterium richness strontium richness strontium fermentating formula liquid, aerobic constant temperature shaken cultivation obtain saccharomycete richness strontium microbial inoculum；
5) rich strontium bacterial manure is prepared:
Photosynthetic bacteria richness strontium microbial inoculum, lactic acid bacteria richness strontium microbial inoculum and saccharomycete richness strontium microbial inoculum are mixed in proportion up to compound rich strontium bacterium
Fertilizer.
Preferably, a kind of preparation method of compound rich strontium bacterial manure, comprising the following steps:
1) prepared by strain activation and culture base:
Photosynthetic bacteria activation medium, lactic acid bacteria activation medium and saccharomycete activation medium are prepared respectively；
Preparation photosynthetic bacteria activation medium: 3~5 g/L of DL-malic acid, 2~4 g/L of yeast powder, peptone 2~4 are weighed
G/L, 0.5~2.5 g/L of ammonium sulfate, 50~100 mg/L of calcium chloride, 100~150 mg/L of magnesium sulfate, sodium acetate 10~
30 mg/L are uniformly mixed, and adjusting PH is 6.5~7.2；
Preparation lactic acid bacteria activation medium: 9 ~ 12 g/L of peptone, 9 ~ 12 g/L of beef extract, yeast powder 3 ~ 7 are weighed
G/L, 15 ~ 20 g/L of glucose, 3 ~ 7 g/L of sodium acetate, 1 ~ 3 g/L of citric acid hydrogen diamine, Tween-80 0.5 ~
1.5mL/L, dipotassium hydrogen phosphate 1 ~ 3 g/L, Fe2(SO4)3 *7 H2O 0.1 ~ 0.3 g/L, MnSO4 *7 H2O 0.03~
0.06 g/L is uniformly mixed, and adjusting PH is 6.2 ~ 6.4；
It prepares saccharomycete activation medium: weighing 200 ~ 240 g/L of potato, 15 ~ 20 g/L of glucose, mixing is equal
It is even；
2) actication of culture:
Original photosynthetic bacteria suspension is inoculated in photosynthetic bacteria activation medium made from step 1) with 10%~20% inoculum concentration,
It is 1000~3000Lx in illumination condition, temperature is 48 ~ 72h of Anaerobic culturel under conditions of 30 ± 2 DEG C, and what is activated is photosynthetic thin
Bacteria suspension；
Picking is stored in 3 ~ 5 single colonie access lactic acid bacteria activation mediums made from step 1) of lactic acid bacteria of MRS culture medium
In, under the conditions of 37 DEG C, anaerobism 24 ~ 48h of constant-temperature shaking culture, the lactic acid bacteria suspension activated；
Picking is stored in saccharomycete activation medium made from 3 ~ 5 single colonie access step 1) of saccharomycete of PDA culture medium,
Under conditions of 30 ± 2 DEG C, aerobic constant temperature shaken cultivation 18 ~ for 24 hours, the yeast bacteria suspension activated；
3) preparation of rich strontium fermentation culture:
Photosynthetic bacteria richness strontium fermentating formula liquid, lactic acid bacteria richness strontium fermentating formula liquid and saccharomycete richness strontium fermentating formula are prepared respectively
Liquid；
Preparation photosynthetic bacteria richness strontium fermentating formula liquid: 3~5 g/L of DL-malic acid, 2~4 g/L of yeast powder, peptone 2 are weighed
~4 g/L, 0.5~2.5 g/L of ammonium sulfate, 50~100 mg/L of calcium chloride, 100~150 mg/L of magnesium sulfate, sodium acetate
10~30 mg/L, 200~500 mg/L of strontium acetate are uniformly mixed, and adjusting PH is 6.5~7.2；
Preparation lactic acid bacteria richness strontium fermentating formula liquid: weighing 20 ~ 30g/L of soy peptide powder, 20 ~ 30g/L of PURE WHEY, and lactose 30 ~
40g/L, 6 ~ 10g/L of sodium acetate, 1 ~ 3g/L of citric acid hydrogen diamine, 0.5 ~ 1.5mL/L of Tween-80,1 ~ 3 g/ of dipotassium hydrogen phosphate
L, Fe2(SO4)3 *7 H2O 0.1 ~ 0.3g/L, MnSO4 *7 H2O 0.03 ~ 0.06 g/L, 0.3 ~ 0.8g/L of L-cysteine,
It is uniformly mixed, adjusting PH is 6.8 ~ 7.0；
It prepares saccharomycete richness strontium fermentating formula liquid: weighing 15 ~ 25g/L of glucose, 8 ~ 10g/L of peptone, 8 ~ 10g/L of yeast powder,
Potassium dihydrogen phosphate 0.1 ~ 0.2g/L, MgSO4*7H20.3 ~ 0.5g/L of O is uniformly mixed, and PH is 6.5 ~ 6.8；
4) rich strontium fermentation prepares rich strontium microbial inoculum:
Rich strontium fermentation, the photosynthetic bacteria, lactic acid bacteria and saccharomycete are carried out to the photosynthetic bacteria, lactic acid bacteria and saccharomycete respectively
The fermentation of rich strontium synchronous carry out:
The fermentation of photosynthetic bacteria richness strontium: by the photosynthetic bacteria suspension of activation with 20%~30% inoculum concentration, being inoculated in content of strontium is 200
It is 1000~2000Lx, the item that temperature is 30 ± 2 DEG C in illumination condition in~500mg/L richness strontium Fermentation by Photosynthetic Bacteria culture medium
72 ~ 120h of Anaerobic culturel is under part to get photosynthetic bacteria richness strontium microbial inoculum；
The fermentation of lactic acid bacteria richness strontium: by the lactic acid bacteria bacteria suspension of activation with 15% ~ 25% inoculum concentration, be inoculated in content of strontium be 50 ~
In 200mg/L richness strontium lactobacillus-fermented culture medium, under the conditions of 37 DEG C, 48 ~ 72h is to get lactic acid bacteria for anaerobism constant-temperature shaking culture
Rich strontium microbial inoculum；
The fermentation of saccharomycete richness strontium: by the saccharomycete bacteria suspension of activation with 20% ~ 25% inoculum concentration, be inoculated in content of strontium be 150 ~
In the rich strontium saccharomycetes to make fermentation culture medium of 300mg/L, under the conditions of 30 ± 2 DEG C, 72 ~ 96h is to get ferment for aerobic constant temperature shaken cultivation
Female bacterium richness strontium microbial inoculum；
5) rich strontium bacterial manure is prepared:
Photosynthetic bacteria richness strontium microbial inoculum, lactic acid bacteria richness strontium microbial inoculum and saccharomycete richness strontium microbial inoculum are mixed in proportion up to compound rich strontium bacterium
Fertilizer.
Preferably, the anaerobism constant temperature oscillation revolving speed is 150 ~ 200r/min.
Preferably, the aerobic constant temperature oscillation revolving speed is 200 ~ 300r/min.
Photosynthetic bacteria is a kind of autotrophic microbe, and reproduction speed is fast, is rich in protein, multivitamin, Co-Q10 and rush
Growth factor etc.；It, can be by the organic matter in soil using light as the energy, and hydrogen sulfide, carbon dioxide and nitrogen etc. are converted to carbohydrate, ammonia
Base acids, vitamins, nitrogen compound, antiviral substance and physiological activator etc. are rich soil and promotion animals and plants
The main power of growth.In addition, the metabolite of photosynthetic bacteria can directly be absorbed by plant, can also be mentioned for other microbial reproductions
For nutrient.
The carbohydrate that the ingestible photosynthetic bacteria of lactic acid bacteria generates forms lactic acid.Lactic acid has very strong sterilizing ability, can be effective
The sharply corruption of harmful microbe growth and breeding and organic matter is inhibited to decompose.
Saccharomycete can utilize amino acid, the carbohydrate and other organic for the secretion, photosynthetic bacteria synthesis that plant root generates
Object generates fermenting power, and synthesis promotes root growth and fissional active material.
Compared with prior art, the invention has the following beneficial effects:
The present invention produces a kind of rich strontium bacterial manure using photosynthetic bacteria, lactic acid bacteria and saccharomycetes to make fermentation, and it is rich both to have can avoid single bacterium
Strontium inhibits the growth and breeding of effective viable bacteria since strontium additive amount is too high, also can avoid leading to agriculture since strontium additive amount is very little
Crop richness strontium is insufficient.Richness strontium bacterial manure produced by the invention can not only promote crops richness strontium, but also can increase soil fertility promotion
Plant growth improves plant disease-resistant ability.In addition, this composite bacterial fertilizer also has centainly the soil of remediating heavy metal and pollution by pesticides
Application value.
Save the cost of the present invention, it is simple to operate, it is high to obtain bacterial manure rate；Triplet fermentor is fermented respectively simultaneously,
Fermentation period can be shortened；Using three kinds of bacterium richness strontiums, single bacterium richness strontium can avoid, if strontium additive amount is too high to inhibit effective viable bacteria
Growth and breeding also can avoid causing crops richness strontium insufficient since strontium additive amount is very little；The rich strontium composite bacterial fertilizer of production is
Organic strontium bacterial manure, is more advantageous to plant growth and development.
Detailed description of the invention
Fig. 1 is method therefor flow chart of the present invention.
Specific embodiment
The present invention is described in further detail combined with specific embodiments below, but protection scope of the present invention is not
Be limited to these embodiments, it is all without departing substantially from the change of present inventive concept or equivalent substitute be included in protection scope of the present invention it
It is interior.
A kind of preparation method of compound rich strontium bacterial manure, comprising the following steps:
1) prepared by strain activation and culture base:
Photosynthetic bacteria activation medium, lactic acid bacteria activation medium and saccharomycete activation medium are prepared respectively；
Preparation photosynthetic bacteria activation medium: 3~5 g/L of DL-malic acid, 2~4 g/L of yeast powder, peptone 2~4 are weighed
G/L, 0.5~2.5 g/L of ammonium sulfate, 50~100 mg/L of calcium chloride, 100~150 mg/L of magnesium sulfate, sodium acetate 10~
30 mg/L are uniformly mixed, and adjusting PH is 6.5~7.2；
Preparation lactic acid bacteria activation medium: 9 ~ 12 g/L of peptone, 9 ~ 12 g/L of beef extract, yeast powder 3 ~ 7 are weighed
G/L, 15 ~ 20 g/L of glucose, 3 ~ 7 g/L of sodium acetate, 1 ~ 3 g/L of citric acid hydrogen diamine, Tween-80 0.5 ~
1.5mL/L, dipotassium hydrogen phosphate 1 ~ 3 g/L, Fe2(SO4)3 *7 H2O 0.1 ~ 0.3 g/L, MnSO4 *7 H2O 0.03~
0.06 g/L is uniformly mixed, and adjusting PH is 6.2 ~ 6.4；
It prepares saccharomycete activation medium: weighing 200 ~ 240 g/L of potato, 15 ~ 20 g/L of glucose, mixing is equal
It is even；
2) actication of culture:
Original photosynthetic bacteria suspension is inoculated in photosynthetic bacteria activation medium made from step 1) with 10%~20% inoculum concentration,
It is 1000~3000Lx in illumination condition, temperature is 48 ~ 72h of Anaerobic culturel under conditions of 30 ± 2 DEG C, and what is activated is photosynthetic thin
Bacteria suspension；
Picking is stored in 3 ~ 5 single colonie access lactic acid bacteria activation mediums made from step 1) of lactic acid bacteria of MRS culture medium
In, under the conditions of 37 DEG C, anaerobism 24 ~ 48h of constant-temperature shaking culture, the lactic acid bacteria suspension activated；
Picking is stored in saccharomycete activation medium made from 3 ~ 5 single colonie access step 1) of saccharomycete of PDA culture medium,
Under conditions of 30 ± 2 DEG C, aerobic constant temperature shaken cultivation 18 ~ for 24 hours, the yeast bacteria suspension activated；
3) preparation of rich strontium fermentation culture:
Photosynthetic bacteria richness strontium fermentating formula liquid, lactic acid bacteria richness strontium fermentating formula liquid and saccharomycete richness strontium fermentating formula are prepared respectively
Liquid；
Preparation photosynthetic bacteria richness strontium fermentating formula liquid: 3~5 g/L of DL-malic acid, 2~4 g/L of yeast powder, peptone 2 are weighed
~4 g/L, 0.5~2.5 g/L of ammonium sulfate, 50~100 mg/L of calcium chloride, 100~150 mg/L of magnesium sulfate, sodium acetate
10~30 mg/L, 200~500 mg/L of strontium acetate are uniformly mixed, and adjusting PH is 6.5~7.2；
Preparation lactic acid bacteria richness strontium fermentating formula liquid: weighing 20 ~ 30g/L of soy peptide powder, 20 ~ 30g/L of PURE WHEY, and lactose 30 ~
40g/L, 6 ~ 10g/L of sodium acetate, 1 ~ 3g/L of citric acid hydrogen diamine, 0.5 ~ 1.5mL/L of Tween-80,1 ~ 3 g/ of dipotassium hydrogen phosphate
L, Fe2(SO4)3 *7 H2O 0.1 ~ 0.3g/L, MnSO4 *7 H2O 0.03 ~ 0.06 g/L, 0.3 ~ 0.8g/L of L-cysteine,
It is uniformly mixed, adjusting PH is 6.8 ~ 7.0；
It prepares saccharomycete richness strontium fermentating formula liquid: weighing 15 ~ 25g/L of glucose, 8 ~ 10g/L of peptone, 8 ~ 10g/L of yeast powder,
Potassium dihydrogen phosphate 0.1 ~ 0.2g/L, MgSO4*7H20.3 ~ 0.5g/L of O is uniformly mixed, and PH is 6.5 ~ 6.8；
4) rich strontium fermentation prepares rich strontium microbial inoculum:
Rich strontium fermentation, the photosynthetic bacteria, lactic acid bacteria and saccharomycete are carried out to the photosynthetic bacteria, lactic acid bacteria and saccharomycete respectively
The fermentation of rich strontium synchronous carry out:
The fermentation of photosynthetic bacteria richness strontium: by the photosynthetic bacteria suspension of activation with 20%~30% inoculum concentration, being inoculated in content of strontium is 200
It is 1000~2000Lx, the item that temperature is 30 ± 2 DEG C in illumination condition in~500mg/L richness strontium Fermentation by Photosynthetic Bacteria culture medium
72 ~ 120h of Anaerobic culturel is under part to get photosynthetic bacteria richness strontium microbial inoculum；
The fermentation of lactic acid bacteria richness strontium: by the lactic acid bacteria bacteria suspension of activation with 15% ~ 25% inoculum concentration, be inoculated in content of strontium be 50 ~
In 200mg/L richness strontium lactobacillus-fermented culture medium, under the conditions of 37 DEG C, 48 ~ 72h is to get lactic acid bacteria for anaerobism constant-temperature shaking culture
Rich strontium microbial inoculum；
The fermentation of saccharomycete richness strontium: by the saccharomycete bacteria suspension of activation with 20% ~ 25% inoculum concentration, be inoculated in content of strontium be 150 ~
In the rich strontium saccharomycetes to make fermentation culture medium of 300mg/L, under the conditions of 30 ± 2 DEG C, 72 ~ 96h is to get ferment for aerobic constant temperature shaken cultivation
Female bacterium richness strontium microbial inoculum；
5) rich strontium bacterial manure is prepared:
Photosynthetic bacteria richness strontium microbial inoculum, lactic acid bacteria richness strontium microbial inoculum and saccharomycete richness strontium microbial inoculum are mixed in proportion up to compound rich strontium bacterium
Fertilizer.
Preferably, the anaerobism constant temperature oscillation revolving speed is 150 ~ 200r/min.
Preferably, the aerobic constant temperature oscillation revolving speed is 200 ~ 300r/min.
Photosynthetic bacteria is a kind of autotrophic microbe, and reproduction speed is fast, is rich in protein, multivitamin, Co-Q10 and rush
Growth factor etc.；It, can be by the organic matter in soil using light as the energy, and hydrogen sulfide, carbon dioxide and nitrogen etc. are converted to carbohydrate, ammonia
Base acids, vitamins, nitrogen compound, antiviral substance and physiological activator etc. are rich soil and promotion animals and plants
The main power of growth.In addition, the metabolite of photosynthetic bacteria can directly be absorbed by plant, can also be mentioned for other microbial reproductions
For nutrient.
The carbohydrate that the ingestible photosynthetic bacteria of lactic acid bacteria generates forms lactic acid.Lactic acid has very strong sterilizing ability, can be effective
The sharply corruption of harmful microbe growth and breeding and organic matter is inhibited to decompose.
Saccharomycete can utilize amino acid, the carbohydrate and other organic for the secretion, photosynthetic bacteria synthesis that plant root generates
Object generates fermenting power, and synthesis promotes root growth and fissional active material.
The photosynthetic bacteria that the present invention chooses, lactic acid bacteria and saccharomycete have synergistic effect, this three kinds of bacterium have enrichment to strontium
Ability, absorb be enriched with strontium during, the strontium of mineralized is converted into the strontium for the organic state for being easy to be absorbed by crops,
Single bacterium is limited to the accumulation ability of strontium, is not able to satisfy plant growth demand, and the present invention selects three kinds of bacterium synergistic effects；Three association
Same-action is additionally beneficial to boot environment micropopulation to good direction activity while supplementing strontium to crop, and inhibits corruption
The proliferation of bacterium, pathogeny bacterium, to play the effect for improving environment, improvement soil, purifying water, eliminate stink, inhibiting corruption.
But since the condition of culture of three kinds of bacterium is different, we are fermented respectively simultaneously using three Zymolysis Equipment.
The following are fermentation process of the invention is as shown in Figure 1.
The entire fermentation process automation control of the present invention, it is cost-saved, it is simple to operate, it is high to obtain bacterial manure rate；Three
Fermentor is fermented respectively simultaneously, can shorten fermentation period；Using three kinds of bacterium richness strontiums, single bacterium richness strontium can avoid, if strontium adds
The too high growth and breeding that can inhibit effective viable bacteria of dosage also can avoid leading to crops richness strontium not since strontium additive amount is very little
Foot；The rich strontium composite bacterial fertilizer of production is organic strontium bacterial manure, is more advantageous to plant growth and development.
The present invention is not limited by embodiment illustrated herein, and is to fit to and principles disclosed herein and novelty
The consistent widest range of feature.
Claims (7)
1. a kind of preparation method of compound rich strontium bacterial manure, which comprises the following steps:
1) prepared by strain activation and culture base:
Photosynthetic bacteria activation medium, lactic acid bacteria activation medium and saccharomycete activation medium are prepared respectively；
2) actication of culture:
Original photosynthetic bacteria suspension is inoculated in photosynthetic bacteria activation medium made from step 1), illumination Anaerobic culturel obtains
The photosynthetic bacteria suspension of activation；
Picking is stored in the lactic acid bacteria single colonie access of MRS culture medium in the lactic acid bacteria activation medium made from step 1), detests
Oxygen constant-temperature shaking culture, the lactic acid bacteria suspension activated；
Picking is stored in saccharomycete activation medium made from the saccharomycete single colonie access step 1) of PDA culture medium, aerobic
Constant-temperature shaking culture, the yeast bacteria suspension activated；
3) preparation of rich strontium fermentation medium:
Photosynthetic bacteria richness strontium fermentation medium, lactic acid bacteria richness strontium fermentation medium and saccharomycete richness strontium fermented and cultured are prepared respectively
Base；
4) rich strontium fermentation prepares rich strontium microbial inoculum:
Rich strontium fermentation, the photosynthetic bacteria, lactic acid bacteria and saccharomycete are carried out to the photosynthetic bacteria, lactic acid bacteria and saccharomycete respectively
The fermentation of rich strontium synchronous carry out:
The fermentation of photosynthetic bacteria richness strontium: the photosynthetic bacteria suspension of activation is inoculated in photosynthetic bacteria richness strontium fermentation medium, in light
The Anaerobic culturel according under the conditions of obtains photosynthetic bacteria richness strontium microbial inoculum；
The fermentation of lactic acid bacteria richness strontium: by the lactic acid bacteria bacterial suspension inoculation of activation in lactic acid bacteria richness strontium fermentation medium, anaerobism constant temperature
Shaken cultivation obtains lactic acid bacteria richness strontium microbial inoculum；
The fermentation of saccharomycete richness strontium: by the saccharomycete bacterial suspension inoculation of activation in saccharomycete richness strontium fermentation medium, aerobic constant temperature
Shaken cultivation obtains saccharomycete richness strontium microbial inoculum；
5) rich strontium bacterial manure is prepared:
Photosynthetic bacteria richness strontium microbial inoculum, lactic acid bacteria richness strontium microbial inoculum and saccharomycete richness strontium microbial inoculum are mixed in proportion up to compound rich strontium bacterium
Fertilizer.
2. the preparation method of the compound rich strontium bacterial manure of one kind according to claim 1, which comprises the following steps:
1) prepared by strain activation and culture base:
Photosynthetic bacteria activation medium, lactic acid bacteria activation medium and saccharomycete activation medium are prepared respectively；
Preparation photosynthetic bacteria activation medium: 3~5 g/L of DL-malic acid, 2~4 g/L of yeast powder, peptone 2~4 are weighed
G/L, 0.5~2.5 g/L of ammonium sulfate, 50~100 mg/L of calcium chloride, 100~150 mg/L of magnesium sulfate, sodium acetate 10~
30 mg/L are uniformly mixed, and adjusting PH is 6.5~7.2；
Preparation lactic acid bacteria activation medium: 9 ~ 12 g/L of peptone, 9 ~ 12 g/L of beef extract, yeast powder 3 ~ 7 are weighed
G/L, 15 ~ 20 g/L of glucose, 3 ~ 7 g/L of sodium acetate, 1 ~ 3 g/L of citric acid hydrogen diamine, Tween-80 0.5 ~
1.5mL/L, dipotassium hydrogen phosphate 1 ~ 3 g/L, Fe2(SO4)3 *7 H2O 0.1 ~ 0.3 g/L, MnSO4 *7H2O 0.03~0.06
G/L is uniformly mixed, and adjusting PH is 6.2 ~ 6.4；
It prepares saccharomycete activation medium: weighing 200 ~ 240 g/L of potato, 15 ~ 20 g/L of glucose, mixing is equal
It is even；
2) actication of culture:
Original photosynthetic bacteria suspension is inoculated in photosynthetic bacteria activation medium made from step 1) with 10%~20% inoculum concentration,
It is 1000~3000Lx in illumination condition, temperature is 48 ~ 72h of Anaerobic culturel under conditions of 30 ± 2 DEG C, and what is activated is photosynthetic thin
Bacteria suspension；
Picking is stored in 3 ~ 5 single colonie access lactic acid bacteria activation mediums made from step 1) of lactic acid bacteria of MRS culture medium
In, under the conditions of 37 DEG C, anaerobism 24 ~ 48h of constant-temperature shaking culture, the lactic acid bacteria suspension activated；
Picking is stored in saccharomycete activation medium made from 3 ~ 5 single colonie access step 1) of saccharomycete of PDA culture medium,
Under conditions of 30 ± 2 DEG C, aerobic constant temperature shaken cultivation 18 ~ for 24 hours, the yeast bacteria suspension activated；
3) preparation of rich strontium fermentation medium:
Photosynthetic bacteria richness strontium fermentation medium, lactic acid bacteria richness strontium fermentation medium and saccharomycete richness strontium fermented and cultured are prepared respectively
Base；
Preparation photosynthetic bacteria richness strontium fermentation medium: 3~5 g/L of DL-malic acid, 2~4 g/L of yeast powder, peptone 2 are weighed
~4 g/L, 0.5~2.5 g/L of ammonium sulfate, 50~100 mg/L of calcium chloride, 100~150 mg/L of magnesium sulfate, sodium acetate
10~30 mg/L, 200~500 mg/L of strontium acetate are uniformly mixed, and adjusting PH is 6.5~7.2；
Preparation lactic acid bacteria richness strontium fermentation medium: weighing 20 ~ 30g/L of soy peptide powder, 20 ~ 30g/L of PURE WHEY, and lactose 30 ~
40g/L, 6 ~ 10g/L of sodium acetate, 1 ~ 3g/L of citric acid hydrogen diamine, 0.5 ~ 1.5mL/L of Tween-80,1 ~ 3 g/ of dipotassium hydrogen phosphate
L, Fe2(SO4)3 *7 H2O 0.1 ~ 0.3g/L, MnSO4 *7 H2O 0.03 ~ 0.06 g/L, 0.3 ~ 0.8g/L of L-cysteine,
It is uniformly mixed, adjusting PH is 6.8 ~ 7.0；
It prepares saccharomycete richness strontium fermentation medium: weighing 15 ~ 25g/L of glucose, 8 ~ 10g/L of peptone, 8 ~ 10g/L of yeast powder,
Potassium dihydrogen phosphate 0.1 ~ 0.2g/L, MgSO4*7H20.3 ~ 0.5g/L of O is uniformly mixed, and PH is 6.5 ~ 6.8；
4) rich strontium fermentation prepares rich strontium microbial inoculum:
Rich strontium fermentation, the photosynthetic bacteria, lactic acid bacteria and saccharomycete are carried out to the photosynthetic bacteria, lactic acid bacteria and saccharomycete respectively
The fermentation of rich strontium synchronous carry out:
The fermentation of photosynthetic bacteria richness strontium: by the photosynthetic bacteria suspension of activation with 20%~30% inoculum concentration, being inoculated in content of strontium is 200
It is 1000~2000Lx, the item that temperature is 30 ± 2 DEG C in illumination condition in~500mg/L photosynthetic bacteria richness strontium fermentation medium
72 ~ 120h of Anaerobic culturel is under part to get photosynthetic bacteria richness strontium microbial inoculum；
The fermentation of lactic acid bacteria richness strontium: by the lactic acid bacteria bacteria suspension of activation with 15% ~ 25% inoculum concentration, be inoculated in content of strontium be 50 ~
In 200mg/L lactic acid bacteria richness strontium fermentation medium, under the conditions of 37 DEG C, 48 ~ 72h is to get lactic acid bacteria for anaerobism constant-temperature shaking culture
Rich strontium microbial inoculum；
The fermentation of saccharomycete richness strontium: by the saccharomycete bacteria suspension of activation with 20% ~ 25% inoculum concentration, be inoculated in content of strontium be 150 ~
In the saccharomycete richness strontium fermentation medium of 300mg/L, under the conditions of 30 ± 2 DEG C, 72 ~ 96h is to get ferment for aerobic constant temperature shaken cultivation
Female bacterium richness strontium microbial inoculum；
5) rich strontium bacterial manure is prepared:
Photosynthetic bacteria richness strontium microbial inoculum, lactic acid bacteria richness strontium microbial inoculum and saccharomycete richness strontium microbial inoculum are mixed in proportion up to compound rich strontium bacterium
Fertilizer.
3. the preparation method of the compound rich strontium bacterial manure of one kind according to claim 2, which is characterized in that the anaerobism constant temperature vibration
Swinging revolving speed is 150 ~ 200r/min.
4. the preparation method of the compound rich strontium bacterial manure of one kind according to claim 2, which is characterized in that the aerobic constant temperature vibration
Swinging revolving speed is 200 ~ 300r/min.
5. according to claim 1 or the preparation method of 2 any compound rich strontium bacterial manure of described one kind, which is characterized in that the cream
Sour bacterium activation medium is the MRS fluid nutrient medium without agar.
6. according to claim 1 or the preparation method of 2 any compound rich strontium bacterial manure of described one kind, which is characterized in that the ferment
Female bacterium activation medium is the PDA liquid medium without agar.
7. compound rich strontium bacterial manure made from -6 any preparation methods according to claim 1.
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| CN106399173A (en) * | 2016-09-21 | 2017-02-15 | 光明乳业股份有限公司 | Cheese fermentation agent, and preparation method thereof |
| CN107868778A (en) * | 2017-11-07 | 2018-04-03 | 常州禾吉纺织品有限公司 | A kind of preparation method of Se-enriched yeast |
| CN108424274A (en) * | 2018-05-28 | 2018-08-21 | 张森森 | A kind of rich strontium type microbial-bacterial fertilizer suitable for leaf vegetables |
| CN109182222A (en) * | 2018-10-25 | 2019-01-11 | 山西龙盘微生物科技有限公司 | The method of heavy metal cadmium in selenium-rich microbial agent preparation and its degradation soil and crops |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399173A (en) * | 2016-09-21 | 2017-02-15 | 光明乳业股份有限公司 | Cheese fermentation agent, and preparation method thereof |
| CN107868778A (en) * | 2017-11-07 | 2018-04-03 | 常州禾吉纺织品有限公司 | A kind of preparation method of Se-enriched yeast |
| CN108424274A (en) * | 2018-05-28 | 2018-08-21 | 张森森 | A kind of rich strontium type microbial-bacterial fertilizer suitable for leaf vegetables |
| CN109182222A (en) * | 2018-10-25 | 2019-01-11 | 山西龙盘微生物科技有限公司 | The method of heavy metal cadmium in selenium-rich microbial agent preparation and its degradation soil and crops |
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Application publication date: 20190517 |