CN109757625A - A kind of growth of raising Cuscuta japonicoa and the feed addictive of disease resistance and preparation method thereof - Google Patents
A kind of growth of raising Cuscuta japonicoa and the feed addictive of disease resistance and preparation method thereof Download PDFInfo
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- CN109757625A CN109757625A CN201910107497.XA CN201910107497A CN109757625A CN 109757625 A CN109757625 A CN 109757625A CN 201910107497 A CN201910107497 A CN 201910107497A CN 109757625 A CN109757625 A CN 109757625A
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- 241000207901 Cuscuta Species 0.000 title abstract description 44
- 208000035240 Disease Resistance Diseases 0.000 title description 5
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- 201000010099 disease Diseases 0.000 claims abstract description 32
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 32
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 28
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 24
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000011709 vitamin E Substances 0.000 claims abstract description 24
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 24
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- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 1
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 description 1
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- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention relates to feed additive fields, and in particular to a kind of preparation method for the feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance.Its by weight percentage, the feed addictive include: originate from fermentation of Aspergillus niger production lipase 2%-5%, emulsifier 10%, bacillus amyloliquefaciens 10%-20%, vitamin E 5 10%, lysine 5%, arginine 10%, remaining is carrier.Feed addictive according to the present invention improves the growth performance and survival rate of Cuscuta japonicoa extremely significantly, to significantly improve economic benefit.
Description
Technical field
The invention belongs to field of feed additive technology, and in particular to a kind of raising Cuscuta japonicoa growth and disease resistance
Feed addictive and preparation method thereof.
Background technique
Lipase is also known as triacylglycerol Acyl- hydrolase, is a kind of enzyme decomposed with synthctic fat, it can not only be catalyzed
Ester hydrolysis reaction can also be catalyzed ester synthesis reaction and transesterification reaction.Lipase belongs to a kind of digestive ferment, Exogenous fatty need through
Crossing after lipase digestive is decomposed into free fatty acid, glycerol and monoglyceride could be utilized by animal, the storage of body fat
It also can't do without lipase with employing, lipase also participates in cellular lipid metabolism.In recent years, lipase is because of its unique physiology function
Can with effect and by largely be used in animal and fowl fodder in.And in aquiculture animal, especially current China's high value fingerling, such as
Cuscuta japonicoa, in the cultivation of European eel etc., research and application about lipase are also rarely reported, and this is mainly due to these cultivation
Fish lack the differentiation of stomach during digestive system development, and alimentary canal is mostly the environment of partial neutral, this have with livestock and poultry compared with
Big difference causes the research and development for making it suitable for the lipase that it is used more more complex than livestock and poultry.Lipase can promote
Fat releases fatty acid, while supplementing the nutriments such as part essential fatty acid, improves meat, reduces the wind of fatty,fiss liver
Danger ensures health, improves young survival rate and production performance.In addition to this, the processing technology of aquatic feeds also with animal and fowl fodder
Processing technology have very big difference, this also requires have the lipase for being suitable for Aquatic Feed Processing technique.
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) are aerobic gram positive bacteria, in nature point
Cloth is extensive, free from environmental pollution to people and animals' nonhazardous.Its metabolite enriches, have stronger resistance, growth is fast, stability is good,
Generate antibacterial substance；Destroy the biofilm of bacterium；Adjust animal immunizing power.It is increasingly taken seriously in animal productiong,
It is slightly more to the research of Ba in aquatic animal cultivation, it is found that it can significantly increase fish disease resistance, improves survival rate.Selim will
Ba concentration is 1 × 106CFU/g (G3 group) and 1 × 104CFU/g (G2 group) is added in tilapia feed, as a result feeds G3 after 15d
The fish serum sterilizing ability of group significantly improves, Bacteriolytic activity and NO analysis result are also apparently higher than G2 group, after 30d, G3 group and G2
The fish of the group index increases more, is decreased obviously in test latter stage great-hearted phagocyte percentage, G1, G2, G3 group fish kidney
The mRNA level in-site of dirty IL-1 and TNF-α successively increases, after attacking poison with Luke that yersinia enterocolitica (Y.ruckeri) or C.perfringens
The fish survival rate for feeding bacillus amyloliquefaciens is higher, illustrates that Ba can enhance the immune and disease resistance of bolti.Das etc.
Test discovery Ba can produce anti-Wdwardsiella tarda (E.tarda), Aeromonas hydrophila (Aeromonas in vitro
Hydrophila), the inhibition zone of vibrio parahaemolytious (Vibrio parahaemolyticus) and Vibrio harveyi (V.hatveyi).
After testing determining Ba to the safety of Catla catla ratio, with 1 × 109、1×108、1×107Ba is added to by the concentration of CFU/g
In feed, than detecting behind 4 weeks and 8 weeks, discovery bacillus amyloliquefaciens can be mentioned obviously the Catla catla that feeding weight is 25~30g
The yield of the superoxide anion of high test fish and NO improve myeloperoxidase and total protein concentration, lysozyme activity, improve it
To antibacterial ability, concentration is 1 × 109Effect is most obvious when CFU/g.
Summary of the invention
The object of the present invention is to provide a kind of raising Cuscuta japonicoa growth and the feed addictives of disease resistance, remarkably promote day
The healthy growth and development of this eel effectively improves body's immunity, and has no toxic side effect.
A second object of the present invention is to provide the systems of a kind of raising Cuscuta japonicoa growth and the feed addictive of disease-resistant performance
Preparation Method.
In order to achieve the goal above, the technical scheme adopted by the invention is that:
A kind of feed addictive improving Cuscuta japonicoa growth and disease-resistant performance, the feed addictive use and originate from black song
The lipase of mould fermenting and producing.It is high by fermentation of Aspergillus niger production lipase active, reach 100,000 U/g, there is fabulous hydrolysis rouge
Fat generates unsaturated fatty acid, this is very suitable to Cuscuta japonicoa etc. to the higher fish of unsaturated fatty acid demand content.Addition should
Lipase can make up for it the deficiency of endogenous lipase, improve fish to the digestibility of feed fat.
The bacillus amyloliquefaciens, viable count reach 1.0*108CFU/g, it is free from environmental pollution to people and animals' nonhazardous.
Its metabolite enriches, has that stronger resistance, growth is fast, stability is good, generates antibacterial substance；Destroy the biological quilt of bacterium
Film；Adjust animal immunizing power.
Further, above-mentioned additive feed addictive of the present invention further includes emulsifier, vitamin E, amino acid
And carrier, by weight percentage, each component content are as follows: lipase 2%-5%, emulsifier 10%, bacillus amyloliquefaciens
10%-20%, vitamin E 5 10%, lysine 5%, arginine 10%, remaining is carrier.
Further, the emulsifier is one of sucrose fatty ester, soybean lecithin.Adding emulsifier can be rouge
Good condition is created in the performance of fat enzyme effect.
Further, the vitamin E 5 is a kind of liposoluble vitamin, not only has and promotes fat digestion in feed
The effect of absorption, but also there is preferable antioxidation, the generation of fat oxidation in feed can be prevented, to ensure that
The quality and stability of fat.
In the present invention, lysine plays important function to the growth of fish.It is an important composition of carnitine production
Part.Carnitine is responsible for converting energy for some unsaturated fatty acids, additionally aids reduction cholesterol levels.It can also be with other battalion
It supports and is formed together collagen.Arginine can enhance fish gill tissue physical barriers function, improve phagocyte phagocytic activity and kill
Bacterium activity, improves antibacterial material bactericidal activity and immunoglobulin content, to enhance fish non-specificity and specific immunity function
Energy.Meanwhile arginine has anti-inflammatory effect, and fish body can be protected from the self-inflicted injury of immune response.
Further, the carrier be soluble starch, because its own is not easy to be oxidized, also not with it is other in the present invention
Substance reacts, and is conducive to safeguard stability of the product of the present invention in transport, storage.
The addition of emulsifier and carrier facilitates the dispersion homogeneity and stability of lipase and bacillus amyloliquefaciens.
A kind of preparation method of feed addictive, take respectively lipase, bacillus amyloliquefaciens, emulsifier, vitamin E,
Lysine, arginine and carrier, which are sufficiently mixed and are lyophilized, is made powder to get feed addictive of the present invention is arrived.
Quality adding proportion of the feed addictive in feed is 1.0-2.0%.
The beneficial effects of the present invention are: in the feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the invention,
The lipase of aspergillus niger is originated from, vigor reaches 100,000 U/g, and production cost is low, generates unsaturated lipid with fabulous hydrolyzed fat
Fat acid, this is very suitable to Cuscuta japonicoa etc. to the higher fish of unsaturated fatty acid demand content.Adding the lipase can make up for it
The deficiency of endogenous lipase improves fish to the digestibility of feed fat.Bioavilability is high, drug residue free, nontoxic pair
Effect, it is pollution-free to water body environment, it is easy to use, securely and reliably.Bacillus amyloliquefaciens do not pollute ring to people and animals' nonhazardous
Border.Its metabolite enriches, has that stronger resistance, growth is fast, stability is good, generates antibacterial substance；Destroy the biology of bacterium
Envelope；Cuscuta japonicoa immunity is adjusted, enhances sea eel to the resistivity of pathogenic bacteria, to promote survival rate.
Specific embodiment
The invention will be further described With reference to embodiment.
Reagent used in following embodiment is commercial products.
The present invention utilizes the general character of bacillus amyloliquefaciens, and is not the property of some special bacterial strain, solves starch gemma
The bacterial strain of China typical culture collection center preservation can be used in bacillus, such as CCTCC NO:M2013375 solves starch gemma bar
The bacterial strain of bacterium etc. or the sale of each company, such as Beijing Create Value Biology Co., Ltd..
The lipase for originating from aspergillus niger has following general character: vigor reaches 100,000 U/g, generates with fabulous hydrolyzed fat
Unsaturated fatty acid can make up for it the deficiency of endogenous lipase, improve fish to the digestibility of feed fat.Therefore, this hair
Bright not to be defined to the Aspergillus niger strain for generating lipase, the lipase of any aspergillus niger production is used equally for this hair
It is bright.
Embodiment 1
The feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment, feed addictive is by following component group
At: originate from lipase, bacillus amyloliquefaciens (the CCTCC NO:M of aspergillus niger (Jining Novi believes bioengineering Co., Ltd)
2013375), sucrose fatty ester, vitamin E 5, amino acid, carrier.
The preparation method of the feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment:
100% meter by weight percent, takes lipase 2%, sucrose fatty ester 10%, bacillus amyloliquefaciens respectively
10%, vitamin E 5 10%, lysine 5%, arginine 10%, soluble starch 53% is sufficiently mixed and is lyophilized powdery is made
Object to get arrive feed addictive of the present invention.
The feed addictive of the present embodiment improves the application of the feed of Cuscuta japonicoa growth and disease-resistant performance in preparation, described
Quality adding proportion of the feed addictive in feed is 1.0%.
Embodiment 2
The feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment, feed addictive is by following component group
At: originate from the lipase of aspergillus niger (CCTCC NO:M 206021), bacillus amyloliquefaciens (CCTCC NO:M 2013375),
Sucrose fatty ester, vitamin E 5, amino acid, carrier.
The preparation method of the feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment:
100% meter by weight percent, takes lipase 2%, sucrose fatty ester 10%, bacillus amyloliquefaciens respectively
10%, vitamin E 5 10%, lysine 5%, arginine 10%, soluble starch 53% is sufficiently mixed and is lyophilized powdery is made
Object to get arrive feed addictive of the present invention.
The feed addictive of the present embodiment improves the application of the feed of Cuscuta japonicoa growth and disease-resistant performance in preparation, described
Quality adding proportion of the feed addictive in feed is 2.0%.
Embodiment 3
The feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment, feed addictive is by following component group
At: originate from lipase, bacillus amyloliquefaciens (the CCTCC NO:M of aspergillus niger (Jining Novi believes bioengineering Co., Ltd)
2013375), sucrose fatty ester, vitamin E 5, amino acid, carrier.
The preparation method of the feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment:
100% meter by weight percent, takes lipase 5%, sucrose fatty ester 10%, bacillus amyloliquefaciens respectively
20%, vitamin E 5 10%, lysine 5%, arginine 10%, soluble starch 40% is sufficiently mixed and is lyophilized powdery is made
Object to get arrive feed addictive of the present invention.
The feed addictive of the present embodiment improves the application of the feed of Cuscuta japonicoa growth and disease-resistant performance in preparation, described
Quality adding proportion of the feed addictive in feed is 2.0%.
Embodiment 4
The feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment, feed addictive is by following component group
At: originate from lipase, bacillus amyloliquefaciens (the CCTCC NO:M of aspergillus niger (Jining Novi believes bioengineering Co., Ltd)
2014200), sucrose fatty ester, vitamin E 5, amino acid, carrier.
The preparation method of the feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment:
100% meter by weight percent, takes lipase enzyme 5%, sucrose fatty ester 10%, bacillus amyloliquefaciens respectively
20%, vitamin E 5 10%, lysine 5%, arginine 10%, soluble starch 40% is sufficiently mixed and is lyophilized powdery is made
Object to get arrive feed addictive of the present invention.
The feed addictive of the present embodiment improves the application of the feed of Cuscuta japonicoa growth and disease-resistant performance in preparation, described
Quality adding proportion of the feed addictive in feed is 1.0%.
Embodiment 5
The feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment, feed addictive is by following component group
At: originate from lipase, bacillus amyloliquefaciens (Beijing Lei Li group of aspergillus niger (Jining Novi believes bioengineering Co., Ltd)
Technology R & D Center), sucrose fatty ester, vitamin E 5, amino acid, carrier.
The preparation method of the feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment:
100% meter by weight percent, takes lipase 2%, sucrose fatty ester 10%, bacillus amyloliquefaciens respectively
20%, vitamin E 5 10%, lysine 5%, arginine 10%, soluble starch 43%.It is sufficiently mixed and is lyophilized and powdery is made
Object to get arrive feed addictive of the present invention.
The feed addictive of the present embodiment improves the application of the feed of Cuscuta japonicoa growth and disease-resistant performance in preparation, described
Quality adding proportion of the feed addictive in feed is 1.0%.
Embodiment 6
The feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment, feed addictive is by following component group
At: originate from lipase, the bacillus amyloliquefaciens (CGMCC of aspergillus niger (Jining Novi believes bioengineering Co., Ltd)
No.9649), soybean lecithin, vitamin E 5, amino acid, carrier.
The preparation method of the feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment:
100% meter by weight percent, takes lipase 5% respectively, soybean lecithin 10%, bacillus amyloliquefaciens 10%,
Vitamin E 5 10%, lysine 5%, arginine 10%, soluble starch 50%.It is sufficiently mixed and is lyophilized and powder is made, i.e.,
Obtain feed addictive of the present invention.
The feed addictive of the present embodiment improves the application of the feed of Cuscuta japonicoa growth and disease-resistant performance in preparation, described
Quality adding proportion of the feed addictive in feed is 1.0%.
Embodiment 7
The feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment, feed addictive is by following component group
At: originate from lipase, bacillus amyloliquefaciens (the CCTCC NO:M of aspergillus niger (Jining Novi believes bioengineering Co., Ltd)
2013375), soybean lecithin, vitamin E 5, amino acid, carrier.
The preparation method of the feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment:
100% meter by weight percent, takes lipase 2% respectively, soybean lecithin 10%, bacillus amyloliquefaciens 20%,
Vitamin E 5 10%, lysine 5%, arginine 10%, soluble starch 43%.It is sufficiently mixed and is lyophilized and powder is made, i.e.,
Obtain feed addictive of the present invention.
The feed addictive of the present embodiment improves the application of the feed of Cuscuta japonicoa growth and disease-resistant performance in preparation, described
Quality adding proportion of the feed addictive in feed is 2.0%.
Embodiment 8
The feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment, feed addictive is by following component group
At: originate from lipase, bacillus amyloliquefaciens (the CCTCC NO:M of aspergillus niger (Jining Novi believes bioengineering Co., Ltd)
2013375), soybean lecithin, vitamin E 5, amino acid, carrier.
The preparation method of the feed addictive for improving Cuscuta japonicoa growth and disease-resistant performance of the present embodiment:
100% meter by weight percent, takes lipase 2% respectively, soybean lecithin 10%, bacillus amyloliquefaciens 20%,
Vitamin E 5 10%, lysine 5%, soluble starch 53%.It is sufficiently mixed and is lyophilized and powder is made to get the present invention is arrived
The feed addictive.
The feed addictive of the present embodiment improves the application of the feed of Cuscuta japonicoa growth and disease-resistant performance in preparation, described
Quality adding proportion of the feed addictive in feed is 2.0%.
Using Cuscuta japonicoa as experimental subjects, the performance of the resulting feed addictive of embodiment 1-8 is detected, specific as follows:
1) for trying sea eel: Zhejiang Province, the dam Haining City Ma Jia, Jiaxing riverside cultivation base Cuscuta japonicoa selects 700 tails, is divided into 7
Group.Every group of 700 tails, and counterpoise is not much different (about 20 grams of every tail).
2) application method: blank group uses Fujian heavenly steed eel feed, and it is primary (early to carry out daily feeding by blank group method
Upper 7:00, afternoon 17:00), daily feeding amount is the 4% of fish body weight；Experimental example also presses control group method and carries out feeding, wherein real
Test feed addictive prepared by the embodiment 1 that example 1 is the example addition 1.0% in mass ratio in feed；Experimental example 2 be fishing ground from
The feed addictive that in feed processed prepared by the embodiment 2 of example addition 2.0% in mass ratio；Experimental example 3 is to make feed by oneself in fishing ground
In in mass ratio example addition 2.0% embodiment 3 prepare feed addictive；Experimental example 4 is in fishing ground self-control feed by matter
Feed addictive prepared by the embodiment 4 of amount ratio addition 1.0%；Experimental example 5 is the example in mass ratio in fishing ground self-control feed
Feed addictive prepared by the embodiment 5 of addition 1.0%；Experimental example 6 is that example in mass ratio is added in fishing ground self-control feed
Feed addictive prepared by 1.0% embodiment 6；Experimental example 7 is the example addition 2.0% in mass ratio in fishing ground self-control feed
Feed addictive prepared by embodiment 7；Experimental example 8 is that example adds 2.0% embodiment 8 in mass ratio in fishing ground self-control feed
The feed addictive of preparation；
3) test period: 56 days.
Growth performance test result is as shown in table 1:
1 test result statistical form of table
| Blank example | Experimental example 1 | Experimental example 2 | Experimental example 3 | Experimental example 4 | Experimental example 5 | Experimental example 6 | Experimental example 7 | Experimental example 8 | |
| Initial weight (g) | 20.1±0.2 | 20.4±0.1 | 20.4±0.4 | 19.8±0.2 | 19.4±0.2 | 20.5±0.1 | 20.7±0.2 | 20.3±0.3 | 20.6±0.4 |
| Weight (g) eventually | 35.5±2.4c | 36.5±1.1a | 37.8±1.8b | 39.4±1.4a | 36.5±1.1a | 37.5±1.2a | 38.5±1.1a | 38.1±1.4a | 38.0±1.4a |
| It increases weight (g) | 15.4±1.6c | 16.1±1.5c | 17.0±1.5b | 19.3±1.1a | 17.1±1.3a | 17.0±1.3a | 18.8±1.2a | 17.8±1.5a | 17.4±1.2a |
Aeromonas hydrophila (ATCC strain 7966T) metainfective survival rate:
30 Japanese sea eels are randomly selected from blank example and experimental example respectively, inject Aeromonas hydrophila (ATCC
Strain 7966T), 100 μ L are injected intraperitoneally in every tail fish, and concentration is 1 × 106Cells/mL continues to raise, and observes and records and is real
The survival rate of object is tested, as shown in table 2.
Table 2, each group Cuscuta japonicoa is through Aeromonas hydrophila (ATCC strain 7966T) metainfective survival rate
| Group | Survival rate after 24 hours | Survival rate after 72 hours | Survival rate after 10 days |
| Blank example | 60% | 33.3% | 10% |
| Experimental example 1 | 73.3% | 60% | 53.3% |
| Experimental example 2 | 86.6% | 60% | 56.6% |
| Experimental example 3 | 93.3% | 86.6% | 73.3% |
| Experimental example 4 | 80% | 73.3% | 63.3% |
| Experimental example 5 | 90% | 73.3% | 56.6% |
| Experimental example 6 | 86.6% | 73.3% | 66.7% |
| Experimental example 7 | 86.6% | 80% | 76.7% |
| Experimental example 8 | 90% | 73.3% | 56.6% |
This test result is found through statistical analysis:
Due to not having addition arginine in embodiment 8, there are significant differences with embodiment 6 for overall effect.In the present invention,
Lack any one component, significant difference can be generated to effect, each mutual synergistic of component in the present invention improves Cuscuta japonicoa
Growth and disease-resistant performance.
Experimental example 3, experimental example 6, Cuscuta japonicoa rate of body weight gain is obviously improved compared to blank group in experimental example 7, tests fish
Figure is normal.
Experimental example 3 and experimental example 7 and blank group compare, and the survival rate of Cuscuta japonicoa improves the most significant.
Comprehensive growth performance and disease-resistant performance illustrate in 3 feed addictive preparation method of experimental example and addition mass fraction
The most rationally, Cuscuta japonicoa growth and disease-resistant performance can be significantly improved.
Claims (6)
1. a kind of feed addictive for improving Japanese sea eel growth and disease-resistant performance, which is characterized in that by weight percentage, packet
Include lipase 2%-5%, emulsifier 10%, bacillus amyloliquefaciens 10%-20%, vitamin E 5 10%, lysine 5%, essence
Propylhomoserin 10%, remaining is carrier, and the lipase is using the lipase for originating from fermentation of Aspergillus niger production.
2. feed addictive according to claim 1, which is characterized in that the emulsifier is sucrose fatty ester, soybean
One of phosphatide.
3. feed addictive according to claim 1, which is characterized in that the vitamin E 5 is a kind of fat-soluble dimension life
Element.
4. feed addictive according to claim 1, which is characterized in that the carrier is soluble starch.
5. a kind of preparation method of feed addictive described in claim 1, which is characterized in that take respectively lipase, emulsifier,
Vitamin E 5, arginine, lysine and carrier are sufficiently mixed and are lyophilized and powder is made adds to get to feed of the present invention
Add agent.
6. requiring the application according to claim 5, which is characterized in that quality addition of the feed addictive in feed
Ratio is 1.0%-2.0%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111893066A (en) * | 2020-08-04 | 2020-11-06 | 华南农业大学 | Bacillus amyloliquefaciens SCAU-070 and application thereof |
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| CN105614168A (en) * | 2015-12-29 | 2016-06-01 | 武汉新华扬生物股份有限公司 | Feed additive suitable for stomachless fish and containing high-temperature-resistant neutral lipase and application of feed additive |
| CN107865255A (en) * | 2016-09-26 | 2018-04-03 | 夏安娜 | A kind of eel culture feed |
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| CN102018141A (en) * | 2010-11-19 | 2011-04-20 | 福建天马饲料有限公司 | Anguilla japonica powdery mixed feed |
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Inventor after: Zheng Chengcai Inventor after: Fei Hui Inventor after: Huang Mengmeng Inventor after: Lv Zhengbing Inventor after: Han Bingnan Inventor before: Fei Hui Inventor before: Zheng Chengcai Inventor before: Huang Mengmeng Inventor before: Lv Zhengbing Inventor before: Han Bingnan |
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Application publication date: 20190517 |