CN109303204A - A kind of feed addictive and the preparation method and application thereof for promoting Cuscuta japonicoa growth and improving immunity - Google Patents

A kind of feed addictive and the preparation method and application thereof for promoting Cuscuta japonicoa growth and improving immunity Download PDF

Info

Publication number
CN109303204A
CN109303204A CN201811360125.XA CN201811360125A CN109303204A CN 109303204 A CN109303204 A CN 109303204A CN 201811360125 A CN201811360125 A CN 201811360125A CN 109303204 A CN109303204 A CN 109303204A
Authority
CN
China
Prior art keywords
feed addictive
feed
growth
lipase
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201811360125.XA
Other languages
Chinese (zh)
Inventor
费辉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Huzhou Nanxun Baijiayu Ecological Technology Co ltd
Original Assignee
Hangzhou Bai Anuo Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hangzhou Bai Anuo Biotechnology Co Ltd filed Critical Hangzhou Bai Anuo Biotechnology Co Ltd
Priority to CN201811360125.XA priority Critical patent/CN109303204A/en
Publication of CN109303204A publication Critical patent/CN109303204A/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Animal Husbandry (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Insects & Arthropods (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Birds (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The present invention relates to feed additive fields, and in particular to a kind of preparation method and application for promoting Cuscuta japonicoa growth and the feed addictive for improving immunity.It is by weight percentage, the feed addictive includes: lipase (Lip2) 10%-20% of Yarrowia lipolytica (Yarrowia lipolytica) fermenting and producing, emulsifier 20%, bacillus amyloliquefaciens 20%-40%, vitamin E 10%, remaining is carrier.Feed addictive according to the present invention improves the growth performance and disease resistance of Cuscuta japonicoa extremely significantly, to significantly improve the economic benefit of Cuscuta japonicoa breeding enterprise.

Description

A kind of feed addictive and its preparation side for promoting Cuscuta japonicoa growth and improving immunity Method and application
Technical field
The invention belongs to field of feed additive technology, and in particular to immunity is grown and improved to a kind of promotion Cuscuta japonicoa Feed addictive and the preparation method and application thereof
Background technique
Lipase is also known as triacylglycerol Acyl- hydrolase, is a kind of enzyme decomposed with synthctic fat, it can not only be catalyzed Ester hydrolysis reaction can also be catalyzed ester synthesis reaction and transesterification reaction.Lipase belongs to a kind of digestive ferment, Exogenous fatty need through Crossing after lipase digestive is decomposed into free fatty acid, glycerol and monoglyceride could be utilized by animal, the storage of body fat It also can't do without lipase with employing, lipase also participates in cellular lipid metabolism.In recent years, lipase is because of its unique physiology function Can with effect and by largely be used in animal and fowl fodder in.And in aquiculture animal, especially current China's high value fingerling, such as Cuscuta japonicoa, in the cultivation of European eel etc., research and application about lipase are also rarely reported, and this is mainly due to these cultivation Fish lack the differentiation of stomach during digestive system development, and alimentary canal is mostly the environment of partial neutral, this have with livestock and poultry compared with Big difference causes the research and development for making it suitable for the lipase that it is used more more complex than livestock and poultry.Lipase can promote Fat releases fatty acid, while supplementing the nutriments such as part essential fatty acid, improves meat, reduces the wind of fatty,fiss liver Danger ensures health, improves young survival rate and production performance.In addition to this, the processing technology of aquatic feeds also with animal and fowl fodder Processing technology have very big difference, this also requires have the lipase for being suitable for Aquatic Feed Processing technique.
Bacillus amyloliquefaciens (Bacillus amyloliquefaciens, Ba) are aerobic gram positive bacteria, in nature Boundary is widely distributed, free from environmental pollution to people and animals' nonhazardous.Its metabolite enriches, has stronger resistance, growth fast, stable Property it is good, generate antibacterial substance；Destroy the biofilm of bacterium；Adjust animal immunizing power.Increasingly by weight in animal productiong Depending on, it is slightly more to the research of Ba in aquatic animal cultivation, it is found that it can significantly increase fish disease resistance, improves survival rate. Ba concentration is 1 × 10 by Selim6CFU/g (G3 group) and 1 × 104CFU/g (G2 group) is added in tilapia feed, as a result feeds The fish serum sterilizing ability of G3 group significantly improves after 15d, Bacteriolytic activity and NO analysis result are also apparently higher than G2 group, after 30d, G3 Group and the fish of the G2 group index increase more, are decreased obviously in test latter stage great-hearted phagocyte percentage, G1, G2, G3 The mRNA level in-site of group fish kidney IL-1 and TNF-α successively increases, with Luke that yersinia enterocolitica (Y.ruckeri) or C.perfringens Attack after poison feed bacillus amyloliquefaciens fish survival rate it is higher, illustrate that Ba can enhance the immune and disease-resistant energy of bolti Power.The test discovery Ba of Das etc. can produce anti-Wdwardsiella tarda (E.tarda), Aeromonas hydrophila in vitro (Aeromonas hydrophila), vibrio parahaemolytious (Vibrio parahaemolyticus) and Vibrio harveyi (V.hatveyi) inhibition zone.After testing determining Ba to the safety of Catla catla ratio, with 1 × 109、1×108、1× 107Ba is added in feed by the concentration of CFU/g, and the Catla catla that feeding weight is 25~30g is sent out than detecting behind 4 weeks and 8 weeks Existing bacillus amyloliquefaciens can significantly improve the superoxide anion of test fish and the yield of NO, improve myeloperoxidase and total egg White concentration, lysozyme activity improve it to antibacterial ability, and concentration is 1 × 109Effect is most obvious when CFU/g.
Summary of the invention
The object of the present invention is to provide feed addictive and its preparations of a kind of promotion Cuscuta japonicoa growth and raising immunity Method and application.
The object of the present invention is to provide the feed addictives of a kind of growth of promotion Cuscuta japonicoa and raising immunity, remarkably promote The healthy growth and development of Cuscuta japonicoa effectively improves body's immunity, and has no toxic side effect.
A second object of the present invention is to provide the feed addictives of a kind of promotion Cuscuta japonicoa growth and raising immunity Preparation method.
Promote Cuscuta japonicoa growth in preparation third object of the present invention is to provide a kind of above-mentioned feed addictive and improves The application of the feed of immunity.
In order to achieve the goal above, the technical scheme adopted by the invention is that:
A kind of feed addictive for promoting Cuscuta japonicoa growth and improving immunity, the feed addictive is using solution starch The lipase (Lip2) of bacillus and Yarrowia lipolytica (Yarrowia lipolytica) fermenting and producing.The lipase (Lip2) unsaturated fatty acid, such as docosahexaenoic acid (DHA) and eicosapentaenoic are generated with fabulous hydrolyzed fat Sour (EPA), this is very suitable to Cuscuta japonicoa etc. to the higher fish of unsaturated fatty acid demand content.Adding the lipase can be more The deficiency of endogenous lipase is mended, improves fish to the digestibility of feed fat.The bacillus amyloliquefaciens are in nature It is widely distributed, it is free from environmental pollution to people and animals' nonhazardous.Its metabolite enriches, has stronger resistance, grows fast, stability Good, generation antibacterial substance；Destroy the biofilm of bacterium；Adjust animal immunizing power.The present invention is by bacillus amyloliquefaciens and rouge For both fat enzymes (Lip2) collectively as additive, the unsaturated fatty acid optimization that lipase (Lip2) hydrolysate feed generates solves starch Bacillus growing environment, collaboration promote Cuscuta japonicoa growth, improve immunity.
Further, above-mentioned additive further includes emulsifier, vitamin E, carrier, and by weight percentage, each component contains Amount are as follows: lipase 10%-20%, bacillus amyloliquefaciens 20%-40%, emulsifier 20%, vitamin e1 0%, remaining is load Body.
Further, the emulsifier is one of sucrose fatty ester, soybean lecithin.Adding emulsifier can be rouge Good condition is created in the performance of fat enzyme effect.
Further, the vitamin E is a kind of liposoluble vitamin, not only has and fat digestion in feed is promoted to inhale The effect of receipts, but also there is preferable antioxidation, the generation of fat oxidation in feed can be prevented, to ensure that rouge The quality and stability of fat.
Further, the carrier be soluble starch, because its own is not easy to be oxidized, also not with it is other in the present invention Substance reacts, and is conducive to safeguard stability of the product of the present invention in transport, storage.
A kind of preparation method of feed addictive, which is characterized in that take lipase, bacillus amyloliquefaciens, emulsification respectively Agent, vitamin E and carrier, which are sufficiently mixed and dry, is made powder to get feed addictive of the present invention is arrived.
A kind of feed addictive promotes the application of Cuscuta japonicoa growth and the feed for improving immunity in preparation, and the feed adds Adding quality adding proportion of the agent in feed is 0.5%-1.0%.
In the feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the invention, Yarrowia lipolytica The lipase (Lip2) of (Yarrowia lipolytica) fermenting and producing generates unsaturated fat with fabulous hydrolyzed fat Acid, such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), this is very suitable to Cuscuta japonicoa etc. to unsaturated fat The sour higher fish of demand content.Adding the lipase can make up for it the deficiency of endogenous lipase, improve fish to feed rouge The digestibility of fat.Bioavilability is high, and drug residue free has no toxic side effect, pollution-free to water body environment, easy to use, safety Reliably.Bacillus amyloliquefaciens are free from environmental pollution to people and animals' nonhazardous.Its metabolite enriches, has stronger resistance, growth Fastly, stability is good, generates antibacterial substance；Destroy the biofilm of bacterium；Cuscuta japonicoa immunity is adjusted, enhances sea eel to pathogenic bacteria Resistivity, to promote survival rate.
Detailed description of the invention
Fig. 1 is each group Cuscuta japonicoa through Aeromonas hydrophila (ATCC strain 7966T) metainfective survival rate.
Specific embodiment
The invention will be further described With reference to embodiment
Embodiment 1
The feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment, feed addictive is by following component Composition: the lipase (Lip2) of Yarrowia lipolytica (Yarrowia lipolytica) fermenting and producing, bacillus amyloliquefaciens, Emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment:
100% meter by weight percent, takes lipase (Lip2) 10%, bacillus amyloliquefaciens 20%, Sucrose Fatty Acid Ester respectively Fat acid esters 20%, vitamin E 10%, soluble starch 40%, which is sufficiently mixed and dries, is made powder to get institute of the present invention is arrived The feed addictive stated.
The feed addictive of the present embodiment promotes the application of the feed of Cuscuta japonicoa growth and raising immunity, institute in preparation Stating quality adding proportion of the feed addictive in feed is 1.0%.
Embodiment 2
The feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment, feed addictive is by following component Composition: the lipase (Lip2) of Yarrowia lipolytica (Yarrowia lipolytica) fermenting and producing, bacillus amyloliquefaciens, Emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment:
100% meter by weight percent, takes lipase (Lip2) 10%, bacillus amyloliquefaciens 20%, Sucrose Fatty Acid Ester respectively Fat acid esters 20%, vitamin E 10%, soluble starch 40%, which is sufficiently mixed and dries, is made powder to get institute of the present invention is arrived The feed addictive stated.
The feed addictive of the present embodiment promotes the application of the feed of Cuscuta japonicoa growth and raising immunity, institute in preparation Stating quality adding proportion of the feed addictive in feed is 0.5%.
Embodiment 3
The feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment, feed addictive is by following component Composition: the lipase (Lip2) of Yarrowia lipolytica (Yarrowia lipolytica) fermenting and producing, bacillus amyloliquefaciens, Emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment:
100% meter by weight percent, takes lipase (Lip2) 20%, bacillus amyloliquefaciens 40%, Sucrose Fatty Acid Ester respectively Fat acid esters 20%, 10% soluble starch of vitamin E 10%, which is sufficiently mixed and dries, is made powder to get institute of the present invention is arrived The feed addictive stated.
The feed addictive of the present embodiment promotes the application of the feed of Cuscuta japonicoa growth and raising immunity, institute in preparation Stating quality adding proportion of the feed addictive in feed is 1.0%.
Embodiment 4
The feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment, feed addictive is by following component Composition: the lipase (Lip2) of Yarrowia lipolytica (Yarrowia lipolytica) fermenting and producing, bacillus amyloliquefaciens, Emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment:
100% meter by weight percent, takes lipase (Lip2) 20%, bacillus amyloliquefaciens 40%, Sucrose Fatty Acid Ester respectively Fat acid esters 20%, vitamin E 10%, soluble starch 10%, which is sufficiently mixed and dries, is made powder to get institute of the present invention is arrived The feed addictive stated.
The feed addictive of the present embodiment promotes the application of the feed of Cuscuta japonicoa growth and raising immunity, institute in preparation Stating quality adding proportion of the feed addictive in feed is 0.5%.
Embodiment 5
The feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment, feed addictive is by following component Composition: the lipase (Lip2) of Yarrowia lipolytica (Yarrowia lipolytica) fermenting and producing, bacillus amyloliquefaciens, Emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment:
100% meter by weight percent, takes lipase (Lip2) 10%, bacillus amyloliquefaciens 40%, Sucrose Fatty Acid Ester respectively Fat acid esters 20%, vitamin E 10%, soluble starch 20%, which is sufficiently mixed and dries, is made powder to get institute of the present invention is arrived The feed addictive stated.
The feed addictive of the present embodiment promotes the application of the feed of Cuscuta japonicoa growth and raising immunity, institute in preparation Stating quality adding proportion of the feed addictive in feed is 1.0%.
Embodiment 6
The feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment, feed addictive is by following component Composition: the lipase (Lip2) of Yarrowia lipolytica (Yarrowia lipolytica) fermenting and producing, bacillus amyloliquefaciens, Emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment:
100% meter by weight percent, takes lipase (Lip2) 10%, bacillus amyloliquefaciens 40%, Sucrose Fatty Acid Ester respectively Fat acid esters 20%, vitamin E 10%, soluble starch 20%, which is sufficiently mixed and dries, is made powder to get institute of the present invention is arrived The feed addictive stated.
The feed addictive of the present embodiment promotes the application of the feed of Cuscuta japonicoa growth and raising immunity, institute in preparation Stating quality adding proportion of the feed addictive in feed is 0.5%.
Embodiment 7
The feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment, feed addictive is by following component Composition: the lipase (Lip2) of Yarrowia lipolytica (Yarrowia lipolytica) fermenting and producing, bacillus amyloliquefaciens, Emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment:
100% meter by weight percent, takes bacillus amyloliquefaciens 20%, sucrose fatty ester 20%, vitamin E respectively 10%, soluble starch 50%, which is sufficiently mixed and dries, is made powder to get feed addictive of the present invention is arrived.
The feed addictive of the present embodiment promotes the application of the feed of Cuscuta japonicoa growth and raising immunity, institute in preparation Stating quality adding proportion of the feed addictive in feed is 0.5%.
Embodiment 8
The feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment, feed addictive is by following component Composition: lipase (Lip2), bacillus amyloliquefaciens, emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Cuscuta japonicoa growth and improving immunity of the present embodiment:
100% meter by weight percent, takes bacillus amyloliquefaciens 20%, sucrose fatty ester 20%, vitamin E respectively 10%, soluble starch 50%, which is sufficiently mixed and dries, is made powder to get feed addictive of the present invention is arrived.
The feed addictive of the present embodiment promotes the application of the feed of Cuscuta japonicoa growth and raising immunity in preparation, described Quality adding proportion of the feed addictive in feed is 1.0%.
Using Cuscuta japonicoa as experimental subjects, the performance of the resulting feed addictive of embodiment 1-8 is detected, specific as follows:
1) for trying sea eel: Zhejiang Province Jiaxing dam Haining City Ma Jia riverside cultivation base Cuscuta japonicoa selects 900 tails, is divided into 7 Group.Every group of 700 tails, and counterpoise is not much different (about 18.9 grams of every tail).
2) application method: blank group uses Fujian heavenly steed eel feed, and it is primary (early to carry out daily feeding by blank group method Upper 7:00, afternoon 17:00), daily feeding amount is the 4% of fish body weight；Experimental example also presses control group method and carries out feeding, wherein real Test feed addictive prepared by the embodiment 1 that example 1 is the example addition 1.0% in mass ratio in feed；Experimental example 2 be fishing ground from The feed addictive that in feed processed prepared by the embodiment 2 of example addition 0.5% in mass ratio；Experimental example 3 is to make feed by oneself in fishing ground In in mass ratio example addition 1.0% embodiment 3 prepare feed addictive；Experimental example 4 is in fishing ground self-control feed by matter Feed addictive prepared by the embodiment 4 of amount ratio addition 0.5%；Experimental example 5 is the example in mass ratio in fishing ground self-control feed Feed addictive prepared by the embodiment 5 of addition 1.0%；Experimental example 6 is that example in mass ratio is added in fishing ground self-control feed Feed addictive prepared by 0.5% embodiment 6；Experimental example 7 is the example addition 0.5% in mass ratio in fishing ground self-control feed Feed addictive prepared by embodiment 7；Experimental example 8 is that example adds 1.0% embodiment 8 in mass ratio in fishing ground self-control feed The feed addictive of preparation；
3) test period: 56 days.
Growth performance test result is as shown in table 1:
1 test result statistical form of table
Aeromonas hydrophila (ATCC strain 7966T) metainfective survival rate:
20 Japanese sea eels are randomly selected from blank example and experimental example respectively, inject Aeromonas hydrophila (ATCC Strain 7966T), injection concentration is 1 × 108Cells/mL continues to raise, and observes and record the dead item number of experimental subjects, As shown in Figure 1.
This test result is found through statistical analysis:
Experimental example 1, experimental example 3, Cuscuta japonicoa rate of body weight gain is obviously improved compared to blank group in experimental example 5, tests fish Figure is normal.
Experimental example 3 and experimental example 4 and blank group compare, and the survival rate of Cuscuta japonicoa improves the most significant.
Experimental example 8 and experimental example 2 compare, and lipase (Lip2) and bacillus amyloliquefaciens are combined the growth to Cuscuta japonicoa Synergistic effect can be played.
Comprehensive growth performance and survival rate illustrate in 3 feed addictive preparation method of experimental example and add mass fraction most To be reasonable, Cuscuta japonicoa growth can be remarkably promoted and immunity is promoted.

Claims (8)

1. a kind of feed addictive for promoting Japanese sea eel growth, which is characterized in that the feed addictive is using solution starch The lipase (Lip2) of bacillus and Yarrowia lipolytica (Yarrowia lipolytica) fermenting and producing.
2. feed addictive according to claim 1, which is characterized in that further include emulsifier, vitamin E, carrier；By weight Measure percentages, each component content are as follows: lipase 10%-20%, bacillus amyloliquefaciens 20%-40%, emulsifier 20%, dimension Raw element E 10%, remaining is carrier.
3. feed addictive according to claim 1, which is characterized in that the emulsifier is sucrose fatty ester, soybean One of phosphatide.
4. feed addictive according to claim 1, which is characterized in that the vitamin E is a kind of fat-soluble dimension life Element.
5. feed addictive according to claim 1, which is characterized in that the carrier is soluble starch.
6. a kind of preparation method of feed addictive as claimed in claim 2, which is characterized in that take lipase, solution starch respectively Bacillus, emulsifier, vitamin E and carrier, which are sufficiently mixed and dry, is made powder to get feed of the present invention is arrived Additive.
7. a kind of feed addictive as described in claim 1 promotes the feed of Cuscuta japonicoa growth and raising immunity in preparation Using.
8. requiring the application according to claim 7, which is characterized in that quality addition of the feed addictive in feed Ratio is 0.5%-1.0%.
CN201811360125.XA 2018-11-15 2018-11-15 A kind of feed addictive and the preparation method and application thereof for promoting Cuscuta japonicoa growth and improving immunity Pending CN109303204A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811360125.XA CN109303204A (en) 2018-11-15 2018-11-15 A kind of feed addictive and the preparation method and application thereof for promoting Cuscuta japonicoa growth and improving immunity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201811360125.XA CN109303204A (en) 2018-11-15 2018-11-15 A kind of feed addictive and the preparation method and application thereof for promoting Cuscuta japonicoa growth and improving immunity

Publications (1)

Publication Number Publication Date
CN109303204A true CN109303204A (en) 2019-02-05

Family

ID=65222827

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201811360125.XA Pending CN109303204A (en) 2018-11-15 2018-11-15 A kind of feed addictive and the preparation method and application thereof for promoting Cuscuta japonicoa growth and improving immunity

Country Status (1)

Country Link
CN (1) CN109303204A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111893066A (en) * 2020-08-04 2020-11-06 华南农业大学 Bacillus amyloliquefaciens SCAU-070 and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103478509A (en) * 2013-10-24 2014-01-01 南京农业大学 Production method of bacillus amyloliquefaciens antimicrobial lipopeptide and application thereof in prawn feed
CN105238722A (en) * 2015-11-03 2016-01-13 江苏省苏微微生物研究有限公司 Bacillus amyloliquefaciens strain as well as preparation method and application of strain powder preparation of bacillus amyloliquefaciens strain
CN105614168A (en) * 2015-12-29 2016-06-01 武汉新华扬生物股份有限公司 Feed additive suitable for stomachless fish and containing high-temperature-resistant neutral lipase and application of feed additive
CN106135648A (en) * 2015-04-17 2016-11-23 兰瑛 Lipase compositions and application thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103478509A (en) * 2013-10-24 2014-01-01 南京农业大学 Production method of bacillus amyloliquefaciens antimicrobial lipopeptide and application thereof in prawn feed
CN106135648A (en) * 2015-04-17 2016-11-23 兰瑛 Lipase compositions and application thereof
CN105238722A (en) * 2015-11-03 2016-01-13 江苏省苏微微生物研究有限公司 Bacillus amyloliquefaciens strain as well as preparation method and application of strain powder preparation of bacillus amyloliquefaciens strain
CN105614168A (en) * 2015-12-29 2016-06-01 武汉新华扬生物股份有限公司 Feed additive suitable for stomachless fish and containing high-temperature-resistant neutral lipase and application of feed additive

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111893066A (en) * 2020-08-04 2020-11-06 华南农业大学 Bacillus amyloliquefaciens SCAU-070 and application thereof

Similar Documents

Publication Publication Date Title
CN104431621B (en) A kind of aquatic products compound premix and preparation method thereof
CN102648739A (en) Litopenaeus vannamei compound feed special for winter shed cultivation and preparation method for feed
CN102987096B (en) Microbiological feed additive and preparation method and application thereof
CN105075955B (en) Neat mouth schizothoracin nursery breeding method
CN107529411B (en) Compound feed for soft-shell crabs of scylla paramamosain and preparation method thereof
US10272123B2 (en) Selecting, producing, and feeding whole algae as a feed supplement for cattle and bison to produce meat high in omega 3'S for human health
Megahed et al. Sustainable growth of shrimp aquaculture through biofloc production as alternative to fishmeal in shrimp feeds
CN103210080A (en) Yeast strains and their uses in the production of lipids
GB2437909A (en) Animal feed comprising docosahexaenois acid from a microbial source
CN107319211A (en) A kind of hirudinaria manillensis young perfect compound feed and preparation method thereof
Xiao et al. Effects of the defatted Schizochytrium sp. on growth performance, fatty acid composition, histomorphology and antioxidant status of juvenile mirror carp (Cyprinus carpio var. specularis)
JP6445144B2 (en) Selection, production, and feeding of total algae as feed supplements for cattle and bison to produce omega-3 rich meat for human health
Natsir et al. Utilization of Caulerpa sp. as a feed ingredient for growth and survival of whiteleg shrimp and Chanos chanos in polyculture
CN107404901B (en) Archaebacteria in bioactive animal feed, methods of making compositions and methods of using the compositions
CN106616037A (en) Taurine compound preparation for groupers and application thereof
CN105614168B (en) Feed additive containing high-temperature-resistant neutral lipase and suitable for stomach-free fish and application thereof
CN109303204A (en) A kind of feed addictive and the preparation method and application thereof for promoting Cuscuta japonicoa growth and improving immunity
CN109757625A (en) A kind of growth of raising Cuscuta japonicoa and the feed addictive of disease resistance and preparation method thereof
CN105360679A (en) Feed additive capable of adjusting heat stress broiler nutrient absorption and metabolism
CN104996801A (en) Salmon gairdneri parent fish feed
CN108094714A (en) A kind of feed addictive for promoting Acipenser baeri growth and preparation method and application
CN112913987A (en) Functional feed for improving grass carp quality by using schizochytrium limacinum residues
KR101326267B1 (en) Composition for anti-obesity effect from fermented sea squirt with acid and alkali treatment
CN108902478A (en) A kind of composite feed additive and its application for improving Pelteobagrus fulvidraco immunity
CN104304824B (en) A kind of raw fish of preventing and treating tells material, the feed addictive of alleviation heat stress

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20200525

Address after: No. 1111, beizhuangyang, Jincheng village, Qianjin Town, Nanxun District, Huzhou City, Zhejiang Province

Applicant after: Huzhou Nanxun baijiayu Ecological Technology Co.,Ltd.

Address before: 310000 Workplace 008, Building B5, No. 9, Jiuhuan Road, Jianggan District, Hangzhou City, Zhejiang Province

Applicant before: HANGZHOU BOANNUO BIOLOGICAL TECHNOLOGY Co.,Ltd.

RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20190205