CN108094714A - A kind of feed addictive for promoting Acipenser baeri growth and preparation method and application - Google Patents
A kind of feed addictive for promoting Acipenser baeri growth and preparation method and application Download PDFInfo
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- CN108094714A CN108094714A CN201711245456.4A CN201711245456A CN108094714A CN 108094714 A CN108094714 A CN 108094714A CN 201711245456 A CN201711245456 A CN 201711245456A CN 108094714 A CN108094714 A CN 108094714A
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- feed addictive
- feed
- growth
- lipase
- promoting
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Abstract
The present invention relates to feed additive fields, and in particular to a kind of preparation method and application for the feed addictive that Acipenser baeri is promoted to grow.Its by weight percentage, the feed addictive includes：The lipase (Lip2) of Yarrowia lipolytica (Yarrowialipolytica) fermenting and producing 20% 40%, emulsifier 20%, vitamin E 5 10%, remaining is carrier.Feed addictive pole according to the present invention considerably improves the growth performance of Acipenser baeri and the survival rate of the young, so as to significantly improve economic benefit.
Description
Technical field
The invention belongs to field of feed additive technology, and in particular to a kind of feed for promoting Acipenser baeri growth
Additive and preparation method and application
Background technology
Lipase is also known as triacylglycerol Acyl- hydrolase, is a kind of enzyme decomposed with synthctic fat, it can not only be catalyzed
Ester hydrolysis reaction can also be catalyzed ester synthesis reaction and transesterification reaction.Lipase belongs to a kind of digestive ferment, Exogenous fatty need through
Crossing after lipase digestive is decomposed into free fatty, glycerine and monoglyceride could be utilized by animal, the storage of body fat
It also can't do without lipase with employing, lipase also participates in cellular lipid metabolism.In recent years, lipase is because of its unique physiology work(
It can be largely used in effect in animal and fowl fodder.And in aquiculture animal, especially current China's high value fingerling, such as
In the cultivation of Huso huso, Acipenser baeri etc., research and application on lipase are also rarely reported, and this is mainly due to this
A little cultured fishes lack the differentiation of stomach during digestive system development, and alimentary canal is mostly the environment of partial neutral, this and livestock and poultry
There is larger difference, cause the research and development for being adapted for its lipase used more more complex than livestock and poultry.Lipase can
Fat to be promoted to release aliphatic acid, while the nutriments such as part essential fatty acid are supplemented, improve meat, reduce fatty,fiss
The risk of liver ensures health, improves young survival rate and production performance.In addition, the processing technology of aquatic feeds also with poultry
The processing technology of fowl feed has very big difference, this also requires the lipase for being suitable for Aquatic Feed Processing technique.
The content of the invention
The object of the present invention is to provide a kind of feed addictive and preparation method thereof for promoting Acipenser baeri growth with
Using.
The object of the present invention is to provide a kind of feed addictives for promoting Acipenser baeri growth, remarkably promote western Berli
The healthy growth and development of sub- sturgeon effectively improves body's immunity, and has no toxic side effect.
Second object of the present invention is to provide a kind of preparation side for the feed addictive for promoting Acipenser baeri growth
Method.
Third object of the present invention is to provide a kind of above-mentioned feed addictive and is preparing promotion Acipenser baeri growth
Feed application.
In order to achieve the goal above, the technical solution adopted in the present invention is：
A kind of feed addictive for promoting Acipenser baeri growth, the feed addictive use Yarrowia lipolytica
(Yarrowialipolytica) lipase (Lip2) of fermenting and producing.There is the lipase (Lip2) fabulous hydrolyzed fat to give birth to
Into unrighted acid, such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), this is very suitable for Siberia
The fish higher to unrighted acid demand content such as sturgeon.Endogenous lipase can be made up not by adding the lipase
Foot improves digestibility of the fish to feed fat.
Further, above-mentioned additive includes emulsifier, vitamin E, carrier and the lipase, by weight
Percentages, each component content are：Lipase 20%-40%, emulsifier 20%, vitamin E 5 10%, remaining is carrier.
Further, the emulsifier is one kind in sucrose fatty ester, soybean lecithin.It can be fat to add emulsifier
Good condition is created in the performance of fat enzyme effect.
Further, the vitamin E is a kind of liposoluble vitamin, not only has and fat digestion in feed is promoted to inhale
The effect of receipts, but also with preferable antioxidation, the generation of fat oxidation in feed can be prevented, so as to ensure that fat
The quality and stability of fat.
Further, the carrier be maize cob meal, because its own is not easy to be aoxidized, also not with other objects in the present invention
Matter reacts, and is conducive to safeguard stability of the product of the present invention in transport, storage.
A kind of preparation method of feed addictive, which is characterized in that take lipase, emulsifier, vitamin E and carrier respectively
It is sufficiently mixed and dries and powder is made to get to feed addictive of the present invention.
A kind of feed addictive promotes the application of the feed of Acipenser baeri growth in preparation, and the feed addictive exists
Quality adding proportion in feed is 0.1%-0.5%.
In the feed addictive for promoting Acipenser baeri growth of the present invention, Yarrowia lipolytica
(Yarrowialipolytica) lipase (Lip2) of fermenting and producing has fabulous hydrolyzed fat generation unsaturated fat
Acid, such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), this is very suitable for Acipenser baeri etc. to insatiable hunger
The higher fish with fatty acid requirements content.The deficiency of endogenous lipase can be made up by adding the lipase, improve fish pair
The digestibility of feed fat.Bioavilability is high, and drug residue free has no toxic side effect, pollution-free to water body environment, user
Just, securely and reliably.
Specific embodiment
The invention will be further described With reference to embodiment
Embodiment 1
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 20%, sucrose fatty ester 20%, vitamin E respectively
10%, maize cob meal 50%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.1%.
Embodiment 2
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 30%, sucrose fatty ester 20%, vitamin E respectively
10%, maize cob meal 40%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.1%.
Embodiment 3
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 30%, sucrose fatty ester 20%, vitamin E respectively
10%, maize cob meal 40%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.1%.
Embodiment 4
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 40%, sucrose fatty ester 20%, vitamin E respectively
10%, maize cob meal 30%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.1%.
Embodiment 5
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 20%, sucrose fatty ester 20%, vitamin E respectively
10%, maize cob meal 50%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.5%.
Embodiment 6
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 30%, sucrose fatty ester 20%, vitamin E respectively
10%, maize cob meal 40%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.5%.
Embodiment 7
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 30%, sucrose fatty ester 20%, vitamin E respectively
10%, maize cob meal 40%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.5%.
Embodiment 8
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 40%, sucrose fatty ester 20%, vitamin E respectively
10%, maize cob meal 30%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.5%.
Embodiment 9
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 20% respectively, soybean lecithin 20%, vitamin E 10%,
Maize cob meal 50%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.1%.
Embodiment 10
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 40% respectively, soybean lecithin 20%, vitamin E 10%,
Maize cob meal 30%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.1%.
Embodiment 11
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 20% respectively, soybean lecithin 20%, vitamin E 10%,
Maize cob meal 50%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.5%.
Embodiment 12
The feed addictive for promoting Acipenser baeri growth of the present embodiment, feed addictive consist of the following compositions：
Lipase (Lip2), emulsifier, vitamin E, carrier.
The preparation method of the feed addictive for promoting Acipenser baeri growth of the present embodiment：
100% meter by weight percent, takes lipase (Lip2) 40% respectively, soybean lecithin 20%, vitamin E 10%,
Maize cob meal 30%, which is sufficiently mixed and dries, is made powder to get to feed addictive of the present invention.
The feed addictive of the present embodiment is preparing the application for the feed for promoting Acipenser baeri growth, the feed
Quality adding proportion of the additive in feed is 0.5%.
Using Acipenser baeri as experimental subjects, the performance of the feed addictive of 1~12 gained of detection embodiment, specifically such as
Under：
1) for the sick fish of examination：Qujing City of Yunnan Province Huize County Amur sturgeon group 2 monthly age of cultivation base sturgeon fry, selects
1400 tails are divided into 14 groups.Every group of 100 tails, and counterpoise is not much different (per about 4 grams of tail).
2) application method：Blank group is carried out every using fishing ground self-control feed (crude protein, crude fat etc.) by blank group method
4 it is small when feeding once, daily feeding amount be fish body weight 4%；Experimental example also carries out feeding, wherein experimental example by control group method
1 is to make feed addictive prepared by the embodiment 1 of example addition 0.1% in mass ratio in feed by oneself in fishing ground；Experimental example 2 is in fishing
The feed addictive that in field self-control feed prepared by the embodiment 2 of example addition 0.1% in mass ratio；Experimental example 3 is made by oneself in fishing ground
The feed addictive that in feed prepared by the embodiment 3 of example addition 0.1% in mass ratio；Experimental example 4 is made by oneself in fishing ground in feed
Feed addictive prepared by the embodiment 4 of example addition 0.1% in mass ratio；Experimental example 5 is made by oneself in fishing ground in feed by quality
Feed addictive prepared by the embodiment 5 of ratio addition 0.5%；Experimental example 6 is that example in mass ratio adds in making feed by oneself in fishing ground
Add feed addictive prepared by 0.5% embodiment 6；Experimental example 7 is to make example addition 0.5% in mass ratio in feed by oneself in fishing ground
Embodiment 7 prepare feed addictive；Experimental example 8 is to make the implementation of example addition 0.5% in mass ratio in feed by oneself in fishing ground
Feed addictive prepared by example 8；Experimental example 9 is prepared by the embodiment 9 of the example addition 0.1% in mass ratio in fishing ground self-control feed
Feed addictive；Experimental example 10 is to make feeding prepared by the embodiment 10 of example addition 0.1% in mass ratio in feed by oneself in fishing ground
Feed additives；Experimental example 11 is that the feed for the preparation of embodiment 11 for making example addition 0.5% in mass ratio in feed by oneself in fishing ground adds
Add agent；Experimental example 12 is to make feed addictive prepared by the embodiment 12 of example addition 0.5% in mass ratio in feed by oneself in fishing ground；
3) test period：4 months.
Result of the test is as shown in table 1：
1 result of the test statistical form of table
This result of the test is found through statistical analysis：
Acipenser baeri rate of body weight gain is the most notable in experimental example 8 and experimental example 12, and it is normal to test the build of fish, and reduces
The death rate of the Acipenser baeri young in the breeding process.
Experimental example 8 and experimental example 12 are compared with blank group, the growth-weight gain of Acipenser baeri improve significantly, say
It is bright the most reasonable in experimental example 8 and 12 feed addictive preparation method of experimental example and addition mass fraction, west can be remarkably promoted
The sturgeon growth of Berli Asia.
Claims (8)
1. a kind of feed addictive for promoting Acipenser baeri growth, which is characterized in that the feed addictive is using solution
The lipase (Lip2) of fat Ye Shi yeast (Yarrowialipolytica) fermenting and producing.
2. feed addictive according to claim 1, which is characterized in that including emulsifier, vitamin E, carrier and described
The lipase, by weight percentage, each component content is：Lipase 20%-40%, emulsifier 20%, vitamin
E5 10%, remaining is carrier.
3. feed addictive according to claim 1, which is characterized in that the emulsifier is sucrose fatty ester, soybean
One kind in phosphatide.
4. feed addictive according to claim 1, which is characterized in that the vitamin E is a kind of fat-soluble dimension life
Element.
5. feed addictive according to claim 1, which is characterized in that the carrier is maize cob meal.
6. a kind of preparation method of the feed addictive described in claim 2, which is characterized in that take respectively lipase, emulsifier,
Vitamin E and carrier, which are sufficiently mixed and dry, is made powder to get to feed addictive of the present invention.
7. a kind of feed addictive as described in claim 1 is preparing the application for the feed for promoting Acipenser baeri growth.
8. the application is required according to claim 7, which is characterized in that quality addition of the feed addictive in feed
Ratio is 0.1%-0.5%.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711245456.4A CN108094714A (en) | 2017-12-01 | 2017-12-01 | A kind of feed addictive for promoting Acipenser baeri growth and preparation method and application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711245456.4A CN108094714A (en) | 2017-12-01 | 2017-12-01 | A kind of feed addictive for promoting Acipenser baeri growth and preparation method and application |
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| CN108094714A true CN108094714A (en) | 2018-06-01 |
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| CN201711245456.4A Pending CN108094714A (en) | 2017-12-01 | 2017-12-01 | A kind of feed addictive for promoting Acipenser baeri growth and preparation method and application |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111269919A (en) * | 2020-03-19 | 2020-06-12 | 四川农业大学 | Corn gene ZmLIP2 and application thereof |
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Application publication date: 20180601 |
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