CN109757299A - A kind of cultural method improving lucidum spore powder quality - Google Patents
A kind of cultural method improving lucidum spore powder quality Download PDFInfo
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- CN109757299A CN109757299A CN201910114707.8A CN201910114707A CN109757299A CN 109757299 A CN109757299 A CN 109757299A CN 201910114707 A CN201910114707 A CN 201910114707A CN 109757299 A CN109757299 A CN 109757299A
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Abstract
The invention discloses a kind of cultural method for improving lucidum spore powder quality, include the following steps: generation material bacteria stick and section wood fungus stick three bacteria sticks are tied together one compound new bacteria stick of composition according to generation material bacteria stick-section wood fungus stick-generation material bacteria stick sequence；The compound new bacteria stick is subjected to soil covering culture, then water spray carries out Routine Management, finally collects conidia powder.The present invention can be realized on the basis of spore powder yield remains basically stable, and improve the technical effect of content of polysaccharide in spores powder and conidia powder triterpene content.
Description
Technical field
The present invention relates to agricultural technology fields, more specifically, being related to a kind of cultivation side for improving lucidum spore powder quality
Method.
Background technique
Ganoderma lucidum is Basidiomycetes Polyporaceae Ganoderma fungi, and lucidum spore powder is the essential part of ganoderma lucidum.Ganoderma lucidum spore
Son is the spore that ganoderma lucidum ejects in growth and maturity phase from the cap back side, is the reproduction cell of ganoderma lucidum, medical value positive day
Benefit is taken seriously, and plurality kinds of health care food has been made.The chemical component of Reishi sporule includes the following categories: protein and amino acid
Class, glycopeptide class, vitamins, carrotene, sterols, triterpenes, alkaloids, fatty acid, lactone and inorganic ions class
Deng.
There are two types of existing ganoderma lucidum cultivation methods: substituting stuff cultivation and segment wood cultivated.
Substituting stuff cultivation be with sawdust, cotton seed hulls, sweet wheat bran, the method for the agricultural leftover bits and pieces cultivating ganoderma such as agricultural crop straw,
A large amount of oak can be saved, full of nutrition in compost, culture medium is more loose than timber, so ganoderma lucidum mycelium and fructification are raw
Long fast, growth cycle is short, is only 3-4 months from harvesting is inoculated into, ganoderma lucidum yield is relatively segment wood cultivated higher, and corresponding conidia powder produces
It measures also relatively segment wood cultivated higher；But content of polysaccharide in spores powder and conidia powder triterpene content are relatively segment wood cultivated lower；
Segment wood cultivated is using the method for being suitble to ganoderma lucidum growth tree species to be cut into section cultivating ganoderma, and segment wood cultivated mode needs to disappear
A large amount of a large amount of oaks are consumed, although not needing additional nutrient solution, ratio bacterium germination time, material generation is slow, fructification and spore powder yield
It is lower, but content of polysaccharide in spores powder and conidia powder triterpene content are higher with respect to substituting stuff cultivation.
Summary of the invention
In order to solve the above technical problems, it is an object of the invention to propose a kind of cultivation side for improving lucidum spore powder quality
Method can be realized on the basis of spore powder yield remains basically stable, and improve content of polysaccharide in spores powder and conidia powder triterpene content
Technical effect.
Used technical solution are as follows:
It is a kind of improve lucidum spore powder quality cultural method, include the following steps: by generation material bacteria stick and section wood fungus stick by
According to generation material bacteria stick-section wood fungus stick-generation material bacteria stick sequence, three bacteria sticks are tied together into one compound new bacteria stick of composition；It should
Compound new bacteria stick carries out soil covering culture, then water spray carries out Routine Management, finally collects conidia powder.
Further, by multiple compound new bacteria sticks according to spacing 4-6cm, after line-spacing 8-12cm discharge, earthing 2-3cm
Thickness, water spray, then carries out Routine Management, finally collects conidia powder.
Further, the step of Routine Management includes: when just having gone out sesame, to control the quantity and growth position of ganoderma lucidum,
Ganoderma lucidum is set to grow the reservation on segment wood cultivated material, to pull out by dredging flower bud on long generation material aside.
Further, described section of wood fungus stick the preparation method is as follows:
S1. it selects based on Fagaceae Species, trees select diameter 8-18cm, are cut into the segment of 15-18cm, are packed into polyethylene
Tubular plastic bag, sack are fastened；Normal-pressure sterilization maintains for 24 hours, Temperature fall after bored a period of time is spare at 95-100 DEG C；
S2. routinely sterile working regulation carries out inoculation operation to section wood；It is indoor to be emitted on culture for bacterium bag after inoculation
In layer frame, tube reason is carried out away；A section wood fungus stick can be obtained after mycelia purseful.
Further, generation material bacteria stick the preparation method is as follows:
S1. the preparation of substituting stuff cultivation culture medium: according to sawdust 78%, corn flour 10%, wheat bran 10%, sucrose 1%, gypsum
Add water after 1% weight proportion mixing, makes moisture content in medium 58-62wt%；
S2. prepared cultivation of glossy ganoderma culture medium is subjected to bagging operations, and with lantern ring, cotton plug or with rubber band or rope
Son seals the bacterium bag for completing bagging operations, carries out disinfecting action to bacterium bag thereafter；
S3. routinely sterile working regulation carries out inoculation operation to generation material；It is indoor to be emitted on culture for bacterium bag after inoculation
In layer frame, tube reason is carried out away；Generation material bacteria stick can be obtained after mycelia purseful.
Further, the step of collection conidia powder includes: to carry out conidia powder collection, spore in the ganoderma lucidum fruitbody growth later period
Sub- powder is put up a shed above ganoderma lucidum when collecting, and is then collected with non-woven bag, and then conidia powder is dried or dried, to spore
After powder moisture content is less than 3wt%, packaging storage.
The beneficial effects of the present invention are:
By expecting the sequence of bacteria stick according to generation material bacteria stick-section wood fungus stick-generation, three bacteria sticks are tied together into composition one again
Close new bacteria stick；So that compound new bacteria stick is to press from both sides section wood fungus stick with generation material bacteria stick, this compound new bacteria stick being capable of compound substituting stuff cultivation
With segment wood cultivated advantage；Then compound new bacteria stick is subjected to soil covering culture again, the cultural method of this compound new bacteria stick can
Lucidum spore powder quality is improved, is realized on the basis of spore powder yield remains basically stable, content of polysaccharide in spores powder and spore are improved
The technical effect of powder triterpene content.
Specific embodiment
Below by specific embodiment, the present invention is described in detail, but the purposes of these exemplary embodiments and
Purpose is only used to enumerate the present invention, not constitutes any type of any restriction to real protection scope of the invention, more non-to incite somebody to action
Protection scope of the present invention is confined to this.
Embodiment 1
A kind of cultural method improving lucidum spore powder quality, includes the following steps:
1. lucidum strain is selected: " imperial sesame No.1 "；
2. segment wood cultivated material
It selects based on Fagaceae Species, trees select diameter 8-18cm, are cut into the segment of 15-18cm, are packed into polyethylene cylinder
Shape polybag, sack are fastened.Normal-pressure sterilization maintains for 24 hours, Temperature fall after bored a period of time is spare at 95-100 DEG C.
3. the production of substituting stuff cultivation material
The preparation of substituting stuff cultivation culture medium: by sawdust 78%, corn flour 10%, wheat bran 10%, sucrose 1%, gypsum 1% pressed
Add water after weight proportion mixing, makes moisture content in medium 58-62wt%；Prepared cultivation of glossy ganoderma culture medium is subjected to pack behaviour
Make, pack height as segment wood cultivated material height, and with lantern ring, cotton plug or with rubber band or rope to completion bagging operations
Bacterium bag sealing, thereafter to bacterium bag carry out disinfecting action.
4. bacterium germination
Routinely sterile working regulation carries out inoculation operation to section wood；Bacterium bag after inoculation is emitted on the indoor layer of culture
On frame, tube reason is carried out away；After 15-20 days, same method expects row inoculation operation to generation；Bacterium bag after inoculation is emitted on culture
In indoor layer frame, tube reason is carried out away.
5. going out sesame management
After mycelia purseful, bacterium bag carries out indoor sesame soil covering culture out.The bacterium bag of the bacteria stick of material containing generation and the bacterium of the stick of wood fungus containing section
After the de- bag of bag, according to generation material bacteria stick-section wood fungus stick-generation material bacteria stick sequence, it is one group that every three bacteria sticks, which are tied together, composition
One compound new bacteria stick.Multiple compound new bacteria sticks are according to spacing 5cm, line-spacing 10cm, and after discharging bacterium material, earthing 2cm is thick, water spray,
Carry out Routine Management.When just having gone out sesame, the quantity and growth position of ganoderma lucidum are controlled, ganoderma lucidum grows in segment wood cultivated material (i.e. Duan Mu
Bacteria stick) on reservation, to be pulled out by dredging flower bud on the long material of generation aside (i.e. generation material bacteria stick).
6. conidia powder is collected
Ganoderma lucidum fruitbody grows up the later period into conidia powder collection, and conidia powder is put up a shed above ganoderma lucidum when collecting, and then uses nonwoven
Cloth bag is collected, and then conidia powder is dried or dried, after conidia powder moisture content is less than 3wt%, packaging storage.
Embodiment 2
The cultural method of the present embodiment is implemented referring to embodiment 1, and unlike the first embodiment: lucidum strain is changed to select
With: " celestial sesame No.1 ".
Comparative example 1
A kind of substituting stuff cultivation method, includes the following steps:
1. lucidum strain is selected: " imperial sesame No.1 "；
2. the production of substituting stuff cultivation material
The preparation of substituting stuff cultivation culture medium: by sawdust 78%, corn flour 10%, wheat bran 10%, sucrose 1%, gypsum 1% pressed
Add water after weight proportion mixing, makes moisture content in medium 58-62wt%；Prepared cultivation of glossy ganoderma culture medium is subjected to pack behaviour
Make, pack height as segment wood cultivated material height, and with lantern ring, cotton plug or with rubber band or rope to completion bagging operations
Bacterium bag sealing, thereafter to bacterium bag carry out disinfecting action.
3. bacterium germination
Routinely sterile working regulation carries out inoculation operation to generation material；Bacterium bag after inoculation is emitted on the indoor layer of culture
On frame, tube reason is carried out away.
4. going out sesame management
After mycelia purseful, according to generation material bacteria stick-generation material bacteria stick-generation material bacteria stick sequence after de- bag, by every three bacteria sticks bundle
It is together one group, as material control, bacterium bag carries out indoor sesame soil covering culture out.After the bacterium bag of the bacteria stick of material containing generation takes off bag, generation
Bacteria stick is expected according to spacing 5cm, line-spacing 10cm, and after discharging bacterium material, earthing 2cm is thick, and water spray carries out Routine Management.
5. conidia powder is collected
Ganoderma lucidum fruitbody grows up the later period into conidia powder collection, and conidia powder is put up a shed above ganoderma lucidum when collecting, and then uses nonwoven
Cloth bag is collected, and then conidia powder is dried or dried, after conidia powder moisture content is less than 3wt%, packaging storage.
Comparative example 2
A kind of segment wood cultivated method, includes the following steps:
1. lucidum strain is selected: " imperial sesame No.1 "；
2. segment wood cultivated material
It selects based on Fagaceae Species, trees select diameter 8-18cm, are cut into the segment of 15-18cm, are packed into polyethylene cylinder
Shape polybag, sack are fastened.Normal-pressure sterilization maintains for 24 hours, Temperature fall after bored a period of time is spare at 95-100 DEG C.
3. bacterium germination
Routinely sterile working regulation carries out inoculation operation to section wood；Bacterium bag after inoculation is emitted on the indoor layer of culture
On frame, tube reason is carried out away.
5. going out sesame management
After mycelia purseful, according to section wood fungus stick-section wood fungus stick-section wood fungus stick sequence after de- bag, every three bacteria sticks are tied
Together it is one group, is compareed as section wood, bacterium bag carries out indoor sesame soil covering culture out.The bacterium bag of the stick of wood fungus containing section takes off bag offspring material
Bacteria stick is according to spacing 5cm, line-spacing 10cm, and after discharging bacterium material, earthing 2cm is thick, and water spray carries out Routine Management.
6. conidia powder is collected
Ganoderma lucidum fruitbody grows up the later period into conidia powder collection, and conidia powder is put up a shed above ganoderma lucidum when collecting, and then uses nonwoven
Cloth bag is collected, and then conidia powder is dried or dried, after conidia powder moisture content is less than 3wt%, packaging storage.
Comparative example 3
The substituting stuff cultivation method of this comparative example is implemented referring to comparative example 1, and unlike comparative example 1: lucidum strain is changed to
It selects: " celestial sesame No.1 ".
Comparative example 4
The segment wood cultivated method of this comparative example is implemented referring to comparative example 2, and unlike comparative example 2: lucidum strain is changed to
It selects: " celestial sesame No.1 ".
By embodiment 1-2, the conidia powder that comparative example 1-4 is obtained carries out the test of yield, polyoses content and triterpene content, makes
It is tested with unified conventional test method, obtained data such as the following table 1:
Table 1
As it can be seen from table 1 the spore powder yield that cultural method of the present invention obtains remains basically stable, polyoses content and triterpene contain
Amount improves.Specifically, the yield of compound new bacteria stick is between generation material bacteria stick and section wood fungus stick, but polyoses content and triterpene content
It is all improved relative to generation material bacteria stick and section wood fungus stick.
It should be appreciated that the purposes of these embodiments is merely to illustrate the present invention and is not intended to limit protection model of the invention
It encloses.In addition, it should also be understood that, after reading the technical contents of the present invention, those skilled in the art can make the present invention each
Kind change, modification and/or variation, all these equivalent forms equally fall within guarantor defined by the application the appended claims
Within the scope of shield.
Claims (6)
1. a kind of cultural method for improving lucidum spore powder quality, which comprises the steps of: will be for material bacteria stick and section
Three bacteria sticks are tied together one compound new bacterium of composition according to generation material bacteria stick-section wood fungus stick-generation material bacteria stick sequence by wood fungus stick
Stick；The compound new bacteria stick is subjected to soil covering culture, then water spray carries out Routine Management, finally collects conidia powder.
2. the cultural method according to claim 1 for improving lucidum spore powder quality, which is characterized in that will be multiple described multiple
New bacteria stick is closed according to spacing 4-6cm, after line-spacing 8-12cm discharge, earthing 2-3cm is thick, then water spray carries out Routine Management, finally
Collect conidia powder.
3. the cultural method according to claim 1 for improving lucidum spore powder quality, which is characterized in that the Routine Management
The step of include: just go out sesame when, control the quantity and growth position of ganoderma lucidum, ganoderma lucidum made to grow the reservation on segment wood cultivated material,
To be pulled out by dredging flower bud on long generation material aside.
4. the cultural method according to claim 1 for improving lucidum spore powder quality, which is characterized in that described section of wood fungus stick
The preparation method is as follows:
S1. it selects based on Fagaceae Species, trees select diameter 8-18cm, are cut into the segment of 15-18cm, are packed into polyethylene tubular
Polybag, sack are fastened；Normal-pressure sterilization maintains for 24 hours, Temperature fall after bored a period of time is spare at 95-100 DEG C；
S2. routinely sterile working regulation carries out inoculation operation to section wood；Bacterium bag after inoculation is emitted on the indoor layer frame of culture
On, carry out away tube reason；A section wood fungus stick can be obtained after mycelia purseful.
5. the cultural method according to claim 1 for improving lucidum spore powder quality, which is characterized in that the generation expects bacteria stick
The preparation method is as follows:
S1. the preparation of substituting stuff cultivation culture medium: according to sawdust 78%, corn flour 10%, wheat bran 10%, sucrose 1%, gypsum 1%
Weight proportion mixing after plus water, make moisture content in medium 58-62wt%；
S2. prepared cultivation of glossy ganoderma culture medium is subjected to bagging operations, and with lantern ring, cotton plug or with rubber band or rope pair
The bacterium bag sealing for completing bagging operations, carries out disinfecting action to bacterium bag thereafter；
S3. routinely sterile working regulation carries out inoculation operation to generation material；Bacterium bag after inoculation is emitted on the indoor layer frame of culture
On, carry out away tube reason；Generation material bacteria stick can be obtained after mycelia purseful.
6. the cultural method according to claim 1 for improving lucidum spore powder quality, which is characterized in that the collection spore
The step of powder includes: to carry out conidia powder collection in the ganoderma lucidum fruitbody growth later period, and conidia powder is put up a shed above ganoderma lucidum when collecting, then
It is collected with non-woven bag, then conidia powder is dried or dried, after conidia powder moisture content is less than 3wt%, packaging storage
Hiding.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772281A (en) * | 2021-02-23 | 2021-05-11 | 海南国林沉香村生物科技有限公司 | Planting method of agilawood ganoderma lucidum |
| CN112997804A (en) * | 2021-03-25 | 2021-06-22 | 莆田市农业科学研究所 | Lucid ganoderma sticking and wrapping integrated cut log-imitating cultivation method |
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