CN109744145A - A kind of sapling culture medium Liquid Fertilizer and preparation method thereof - Google Patents
A kind of sapling culture medium Liquid Fertilizer and preparation method thereof Download PDFInfo
- Publication number
- CN109744145A CN109744145A CN201711080266.1A CN201711080266A CN109744145A CN 109744145 A CN109744145 A CN 109744145A CN 201711080266 A CN201711080266 A CN 201711080266A CN 109744145 A CN109744145 A CN 109744145A
- Authority
- CN
- China
- Prior art keywords
- liquid fertilizer
- culture medium
- distilled water
- sapling
- isopentennyladenine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of sapling culture medium Liquid Fertilizers and preparation method thereof and preparation method thereof, which is made of distilled water, glucose, sodium chloride, ammonium nitrate, potassium phosphate, potassium nitrate, vitamin C, indolebutyric acid and isopentennyladenine；Method includes the following steps: carrying out disinfection to sterile vacuum workbench and configuration instrument；Distilled water is added in agitator in aseptic working platform；Stirring in distilled water is added in glucose, chlorination, ammonium nitrate, potassium phosphate, potassium nitrate and vitamin extremely to dissolve；Mixed liquor is stood, is then again stirring for；Indolebutyric acid and isopentennyladenine is added dropwise, stirs evenly and Liquid Fertilizer is made；Liquid Fertilizer is fitted into container, vacuum and low temperature saves.The present invention can provide sufficient nutrient not only for sapling culture medium and can promote sapling cell differentiation and growth, improve the success rate of sapling culture, and preparing process is simple, not be easily introduced germ contamination culture medium using Sterile vacuum operation.
Description
Technical field
The present invention relates to a kind of Liquid Fertilizer, especially a kind of sapling culture medium Liquid Fertilizer and preparation method thereof.
Background technique
Plant Tissue Breeding is called in vitro culture, refers to the tissue isolated and suited the requirements from plant.Organ or cell, it is former
Raw plastid etc. is cultivated under manual control condition by sterile working to obtain regenerated intact plant or production and have
The technology of other products of economic value utilizes plant parts tissue, such as forming layer, parenchymal tissue, mesophyll tissue, endosperm
It carries out culture and obtains regeneration plant, also refer to the culture for generating callus from each organ during the cultivation process, callus is again
By being differentiated to form aftergrowth again.Culture operation, culture medium are carried out using culture medium often carrying out Plant Tissue Breeding
In nutriment need to be added, for plant cell division growth nutrient is provided, at present when carrying out tissue cultures, the Liquid Fertilizer of addition
Or nutriment contained by Liquid Fertilizer is less, it is difficult to provide sufficient nutrient for plant growth, and the operation of adding liquid fertilizer is held
It is easily introduced germ contamination culture medium, plant cell growth differentiation is influenced, tissue cultures is caused to fail.Therefore, existing Liquid Fertilizer
It is less that there are nutritional ingredients, prepares the problem of addition operation is easily introduced germ contamination culture medium, influences the success rate of tissue cultures.
Summary of the invention
The object of the present invention is to provide a kind of sapling culture medium Liquid Fertilizers and preparation method thereof.The present invention can be tree
Seedling culture medium, which provides sufficient nutrient, not only can promote sapling cell differentiation and growth, improve the success rate of sapling culture, and
Preparing process is simple, is not easily introduced germ contamination culture medium using Sterile vacuum operation.
Technical solution of the present invention: a kind of sapling culture medium Liquid Fertilizer, the Liquid Fertilizer are by the raw material of following parts by weight
It is made: 5 ~ 15kg of distilled water, 2 ~ 4kg of glucose, 0.01 ~ 0.05kg of sodium chloride, 0.01 ~ 0.05kg of ammonium nitrate, potassium phosphate 0.01 ~
0.05kg, 0.01 ~ 0.05kg of potassium nitrate, 0.01 ~ 0.05kg of vitamin C, 0.01 ~ 0.05kg of indolebutyric acid, isopentennyladenine
0.01~0.05kg。
In a kind of sapling culture medium Liquid Fertilizer above-mentioned, the Liquid Fertilizer is to be made of raw material from the following weight: being steamed
Distilled water 10kg, glucose 3kg, sodium chloride 0.03kg, ammonium nitrate 0.02kg, potassium phosphate 0.03kg, potassium nitrate 0.03kg, vitamin
C0.04kg, indolebutyric acid 0.02kg, isopentennyladenine 0.03kg.
According to a kind of preparation method of seedling medium fertilizer above-mentioned, this approach includes the following steps；
A: it carries out disinfection to sterile vacuum workbench and configuration instrument.
: distilled water is added in agitator in aseptic working platform.
: stirring in distilled water is added in glucose, sodium chloride, ammonium nitrate, potassium phosphate, potassium nitrate and vitamin and is extremely dissolved.
: mixed liquor is stood into 3 ~ 5h and is again stirring for.
: indolebutyric acid and isopentennyladenine is added dropwise, stirs evenly and Liquid Fertilizer is made.
: Liquid Fertilizer is fitted into container, vacuum and low temperature saves.
The invention has the advantages that providing indispensable nutrition member for plant cell growth by addition glucose and vitamin
Element；By addition sodium chloride, ammonium nitrate, potassium phosphate and potassium nitrate, nitrogen, phosphorus and potassium element can be not only provided for plant cell, and
And water is dissolved in as ionic condition using inorganic salts, it facilitates through cell membrane and is absorbed by plant cell；By adding indolebutyric acid
And isopentennyladenine, promote cell division and differentiation, promotes cell growth；By being configured in gnotobasis, avoid drawing
Enter bacterium, while being saved by vacuum and low temperature, guarantees the activity of Liquid Fertilizer, improve the success rate of tissue cultures.In conclusion this
Invention can provide sufficient nutrient for the culture medium used in tissue cultures and not only can promote plant cell differentiation and growth, raising group
The success rate of culture is knitted, and preparing process is simple, germ contamination culture medium is not easily introduced using Sterile vacuum operation.
Below with reference to embodiment, the present invention is further illustrated, but is not intended as the foundation limited the present invention.
Embodiment 1.A kind of sapling culture medium Liquid Fertilizer, the Liquid Fertilizer are to be made of raw material from the following weight: distillation
Water 10kg, glucose 3kg, sodium chloride 0.03kg, ammonium nitrate 0.02kg, potassium phosphate 0.03kg, potassium nitrate 0.03kg, vitamin
C0.04kg, indolebutyric acid 0.02kg, isopentennyladenine 0.03kg.
The preparation method of above-mentioned sapling culture medium Liquid Fertilizer, this approach includes the following steps；
A: it carries out disinfection to sterile vacuum workbench and configuration instrument.
: distilled water is added in agitator in aseptic working platform.
: stirring in distilled water is added in glucose, chlorination, ammonium nitrate, potassium phosphate, potassium nitrate and vitamin and is extremely dissolved.
: mixed liquor standing 4h is again stirring for.
: indolebutyric acid and isopentennyladenine is added dropwise, stirs evenly and Liquid Fertilizer is made.
: Liquid Fertilizer is fitted into container, vacuum and low temperature saves.
Embodiment 2.A kind of sapling culture medium Liquid Fertilizer, it is characterised in that: the Liquid Fertilizer is by the original of following parts by weight
Material is made: distilled water 5kg, glucose 2kg, sodium chloride 0.01kg, ammonium nitrate 0.01kg, potassium phosphate 0.01kg, potassium nitrate
0.01kg, vitamin C 0.01kg, indolebutyric acid 0.01kg, isopentennyladenine 0.01kg.
The preparation method of above-mentioned seedling medium fertilizer: this approach includes the following steps；
A: it carries out disinfection to sterile vacuum workbench and configuration instrument.
: distilled water is added in agitator in aseptic working platform.
: stirring in distilled water is added in glucose, chlorination, ammonium nitrate, potassium phosphate, potassium nitrate and vitamin and is extremely dissolved.
: mixed liquor standing 3h is again stirring for.
: indolebutyric acid and isopentennyladenine is added dropwise, stirs evenly and Liquid Fertilizer is made.
: Liquid Fertilizer is fitted into container, vacuum and low temperature saves.
Embodiment 3.A kind of sapling culture medium Liquid Fertilizer, the Liquid Fertilizer are to be made of raw material from the following weight: distillation
Water 15kg, glucose 4kg, sodium chloride 0.05kg, ammonium nitrate 0.05kg, potassium phosphate 0.05kg, potassium nitrate 0.05kg, vitamin
C0.05kg, indolebutyric acid 0.05kg, isopentennyladenine 0.05kg.
The preparation method of above-mentioned sapling culture medium Liquid Fertilizer, this approach includes the following steps；
A: it carries out disinfection to sterile vacuum workbench and configuration instrument.
: distilled water is added in agitator in aseptic working platform.
: stirring in distilled water is added in glucose, chlorination, ammonium nitrate, potassium phosphate, potassium nitrate and vitamin and is extremely dissolved.
: mixed liquor standing 5h is again stirring for.
: indolebutyric acid and isopentennyladenine is added dropwise, stirs evenly and Liquid Fertilizer is made.
: Liquid Fertilizer is fitted into container, vacuum and low temperature saves.
Claims (3)
1. a kind of sapling culture medium Liquid Fertilizer, it is characterised in that: the Liquid Fertilizer is to be made of raw material from the following weight: steaming
5 ~ 15kg of distilled water, 2 ~ 4kg of glucose, 0.01 ~ 0.05kg of sodium chloride, 0.01 ~ 0.05kg of ammonium nitrate, 0.01 ~ 0.05kg of potassium phosphate,
0.01 ~ 0.05kg of potassium nitrate, 0.01 ~ 0.05kg of vitamin C, 0.01 ~ 0.05kg of indolebutyric acid, isopentennyladenine 0.01 ~
0.05kg。
2. a kind of sapling culture medium Liquid Fertilizer according to claim 1, it is characterised in that: the Liquid Fertilizer is by following
The raw material of parts by weight is made: distilled water 10kg, glucose 3kg, sodium chloride 0.03kg, ammonium nitrate 0.02kg, potassium phosphate 0.03kg,
Potassium nitrate 0.03kg, vitamin C 0.04kg, indolebutyric acid 0.02kg, isopentennyladenine 0.03kg.
3. a kind of preparation method of sapling culture medium Liquid Fertilizer according to claim 1 or 2, it is characterised in that: the party
Method the following steps are included:
A: it carries out disinfection to sterile vacuum workbench and configuration instrument；
B: distilled water is added in agitator in aseptic working platform；
C: stirring in distilled water is added in glucose, chlorination, ammonium nitrate, potassium phosphate, potassium nitrate and vitamin and is extremely dissolved；
D: mixed liquor is stood into 3 ~ 5h and is again stirring for；
E: being added dropwise indolebutyric acid and isopentennyladenine, stirs evenly and Liquid Fertilizer is made；
F: Liquid Fertilizer is fitted into container, and vacuum and low temperature saves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711080266.1A CN109744145A (en) | 2017-11-06 | 2017-11-06 | A kind of sapling culture medium Liquid Fertilizer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711080266.1A CN109744145A (en) | 2017-11-06 | 2017-11-06 | A kind of sapling culture medium Liquid Fertilizer and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109744145A true CN109744145A (en) | 2019-05-14 |
Family
ID=66401144
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711080266.1A Pending CN109744145A (en) | 2017-11-06 | 2017-11-06 | A kind of sapling culture medium Liquid Fertilizer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109744145A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110327367A (en) * | 2019-06-21 | 2019-10-15 | 北京爱希国际贸易有限公司 | Composition, preparation method, preparation containing nitrate and vitamin and application thereof |
-
2017
- 2017-11-06 CN CN201711080266.1A patent/CN109744145A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110327367A (en) * | 2019-06-21 | 2019-10-15 | 北京爱希国际贸易有限公司 | Composition, preparation method, preparation containing nitrate and vitamin and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102531747A (en) | Compound microbial water-soluble fertilizer and its preparation method | |
| CN105060986A (en) | Fruit swelling type seaweed organic liquid water soluble fertilizer and production method thereof | |
| CN104250172A (en) | Quick collapsed suspension liquid compound fertilizer and preparation method thereof | |
| CN103250556A (en) | Method for cultivating rhodiola rosea bag material healthcare pleurotus nebrodensis | |
| CN104326820A (en) | Integrated special fertilizer for facility cultivation system as well as preparation method and application of integrated special fertilizer | |
| CN106916002A (en) | A kind of organic-inorganic microelement type a great number of elements Liquid Fertilizer and preparation method thereof | |
| CN107721564A (en) | A kind of soilless culture nutrient fluid for increasing production cucumber and preparation method thereof | |
| CN109744145A (en) | A kind of sapling culture medium Liquid Fertilizer and preparation method thereof | |
| CN107721577A (en) | A kind of plant nutrition liquid used for Gynura bicolor and preparation method thereof | |
| CN105732176A (en) | Aquiculture nutrient solution for lettuce | |
| CN103787784B (en) | The agent of compound potato growth nutrition recuperating and application thereof | |
| CN109134034A (en) | A kind of culture medium Liquid Fertilizer and preparation method thereof | |
| CN105777321A (en) | Agricultural liquid microbial preparation containing micromolecular water-soluble organic carbon and preparation method for agricultural liquid microbial preparation | |
| CN106831037A (en) | A kind of edible fungus liquid culture growth medium and preparation method thereof | |
| CN107711415A (en) | A kind of soilless culture nutrient fluid for accelerating Chinese rose growth and preparation method thereof | |
| CN107673800A (en) | A kind of soilless culture nutrient fluid for improving Kidney bean yield and preparation method thereof | |
| CN107879795A (en) | A kind of lily nutrient solution for promoting growth and preparation method thereof | |
| CN107750888A (en) | A kind of soilless culture nutrient fluid for promoting chives to take root and preparation method thereof | |
| CN105646031A (en) | Hydroponics nutrient solution of pakchoi cabbage | |
| CN109627108A (en) | A kind of dedicated liquid suspension fertilizer and preparation method thereof containing nitrification inhibitor of leaf vegetables | |
| CN109534886A (en) | A kind of carrot culture medium Liquid Fertilizer and preparation method thereof | |
| CN105732168A (en) | Aquiculture nutrient solution for Indian lettuce | |
| CN105712779A (en) | Hydroponic nutrient solution for leaf lettuce | |
| CN108863489A (en) | A kind of vegetable hydroponic nutrient solution of the nutrient containing organic carbon and preparation method thereof | |
| CN105198538B (en) | Moringa seedling special nutrient fluid and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190514 |