CN109735449A - A kind of online culture apparatus of biological tissue and observation method - Google Patents
A kind of online culture apparatus of biological tissue and observation method Download PDFInfo
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- CN109735449A CN109735449A CN201910174562.0A CN201910174562A CN109735449A CN 109735449 A CN109735449 A CN 109735449A CN 201910174562 A CN201910174562 A CN 201910174562A CN 109735449 A CN109735449 A CN 109735449A
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- culture chamber
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- feed flow
- waste liquid
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Abstract
The invention discloses a kind of online culture apparatus of biological tissue and observation methods, belong to technical field of biological, the technical problem to be solved in the present invention is that slime mould, fungi and other similar biological cell how to be made to carry out cultivating observation online under simple microscope, a kind of technical solution are as follows: the 1. online culture apparatus of biological tissue, including concave slide and coverslip, reeded disc-shaped structure is set centered on concave slide, the top on concave slide and being located at concave slide groove is arranged in coverslip, and concave slide groove and coverslip form culture chamber；It is provided on concave slide for drainage system, includes feed flow subsystem and drain subsystem for drainage system, feed flow subsystem and drain subsystem are connected to culture chamber.2. observation method is cultivated online by a kind of biological tissue, steps are as follows: (1), building culture chamber；(2), closed culture environment is built；(3), it builds for drainage system；(4), it adjusts for draining flow；(5), nutrient solution and waste collection are injected.
Description
Technical field
The present invention relates to biology, medical detection device and technical fields, and specifically a kind of biological tissue cultivates online
Device and observation method.
Background technique
It is most basic research means for the observation of tissue or cell in biology or field of medicaments, it is common practice to will
After tissue or cell to be observed is cultivated in culture vessel, then test serum or cell be transplanted on glass slide in microscope
Under observed.And it is limited to many limits such as high-power microscope objective table, object lens and glass slide distance, glass slide cover-glass thickness
System can not be cultivated online under the microscope, be observed in real time using dedicated culture vessel.But for field of biomedicine one
A little biologies or histiocytic observation and record need lasting for a long time observation and record, this just needs to carry out biology
Line culture observation.For example the present more slime mould class unicellular organism of foreign study is less in China's research, it is raw to its cell
The research of each processes such as length, sclerotium breeding, spore germination requires to be recorded for a long time, and the collection culture and observation of profession
With the equipment country of one without and external more expensive be difficult to obtain.Therefore how to make slime mould, fungi and other similar
Biological cell can cultivate observation online under simple microscope, meet the research and learning and need to be badly in need of in currently available technology
The technical issues of solution.
The patent document of Patent No. CN107653189A discloses a kind of biological tissue's culture apparatus, including glass slide,
Coverslip, culture solution fluid reservoir, inlet tube, outlet tube and waste liquid tank, the glass slide are equipped with rectangle culture chamber, the coverslip
It is located above rectangle culture chamber, rectangle culture chamber side side wall is equipped with several culture chamber inlets, the rectangle culture chamber
Other side side wall is equipped with several culture chamber liquid outlets；The culture solution fluid reservoir by inlet tube and several culture chamber inlets with
The connection of rectangle culture chamber, the waste liquid tank are connect by outlet tube and several culture chamber liquid outlets with rectangle culture chamber.But it should
It is more demanding and structure is complicated, glass slide, coverslip, culture solution fluid reservoir, inlet tube, outlet tube using equipment in technical solution
Custom-made production is required with critical components such as waste liquid tanks, and these components are due to having complicated its volume of construction and general
Glass slide coverslip compare will be very huge；Furthermore feed flow and Drainage tank are also required to special external component and provide, therefore should
Technical solution is appropriate only for the culture observation of specific macro-organism individual, for as slime mould, fungi and other similar biology it is thin
Online culture and observation of born of the same parents' grade under simple microscope, the technical solution have that at high cost and enforcement difficulty is big.
Summary of the invention
Technical assignment of the invention is to provide a kind of online culture apparatus of biological tissue and observation method, to solve how to make
The problem of slime mould, fungi and other similar biological cell can cultivate observation online under simple microscope.
Technical assignment of the invention realizes in the following manner, a kind of online culture apparatus of biological tissue, including recessed glass
Piece and coverslip, reeded disc-shaped structure is set centered on concave slide, and coverslip is arranged on concave slide and is located at concave slide
The top of groove, concave slide groove and coverslip form culture chamber；
It is provided on concave slide for drainage system, includes feed flow subsystem and drain subsystem, feed flow for drainage system
System and drain subsystem are located at the two sides of culture chamber and feed flow subsystem and drain subsystem are connected to culture chamber respectively.
Preferably, it is respectively arranged with culture chamber inlet and culture chamber leakage fluid dram at the opposite two sides of the culture chamber,
Feed flow subsystem is connected to culture chamber by culture chamber inlet, and drain subsystem is connected to culture chamber, training by culture chamber leakage fluid dram
It supports chamber inlet and culture chamber leakage fluid dram is respectively positioned on coverslip and concave slide junction.
Preferably, the junction of the coverslip and concave slide using sealant be tightly connected, only retain culture chamber into
Liquid mouth and culture chamber leakage fluid dram, control controlling flow direction is formed for apocenosis passage, by that can prevent liquid from permeating to other directions
Diffusion, it is ensured that the leakproofness of culture chamber.
More preferably, the feed flow subsystem includes nutrient solution liquid cartridge, and nutrient solution liquid cartridge bottom offers feed flow open-work,
Feed flow guiding filter paper is provided between feed flow open-work and culture chamber inlet, feed flow guiding filter paper one end is located at feed flow through-hole position,
The feed flow guiding filter paper other end is located at culture chamber inlet, and feed flow guiding filter paper is for being connected to nutrient solution liquid cartridge and culture
Chamber.
More preferably, the feed flow guiding filter paper is arranged on concave slide and between nutrient solution liquid cartridge and coverslip.
More preferably, the drain subsystem includes waste liquid absorption cell, and the bottom of waste liquid absorption cell offers waste liquid and absorbs thoroughly
Hole, waste liquid, which absorbs, is provided with waste liquid guiding filter paper between open-work and culture solution leakage fluid dram, waste liquid guiding filter paper one end is located at waste liquid
Through-hole position is absorbed, the waste liquid guiding filter paper other end is located at culture solution leakage fluid dram, and waste liquid guiding filter paper is for being connected to waste liquid absorption cell
And culture chamber.
More preferably, the waste liquid guiding filter paper is arranged on concave slide and between waste liquid absorption cell and coverslip.
More preferably, it is respectively arranged with water suction block in the nutrient solution liquid cartridge and waste liquid absorption cell, water suction block is using suction
Square structure body made of water material.
Observation method is cultivated online by a kind of biological tissue, and the method steps are as follows:
(1), it builds culture chamber and determinand is put into culture chamber: biological tissues under test or cell are put into concave slide
In groove, appropriate solution and covered are instilled；
(2), it builds closed culture environment: after instilling the air that liquid is discharged in culture chamber into culture chamber, will cover
Fragmentation and concave slide are sealed by sealant and reserve culture chamber inlet and culture chamber leakage fluid dram；
(3), build for drainage system: the online culture apparatus of biological tissue as claimed in one of claims 1-8 is built
For drainage system；
(4), it adjusts for draining flow: filter paper being guided by feed flow and waste liquid guides needed for width and the length adjustment of filter paper
Draining flow；
(5), it injects nutrient solution and waste collection: injecting nutrient solution in the water suction block in nutrient solution liquid cartridge, adjustment supplies
Liquid guiding filter paper and waste liquid guiding filter paper make entirely to form flowing link for drainage system；
(6), nutrition liquid cartridge and water suction block and feed flow guiding filter paper and waste liquid guiding filter in waste liquid absorption cell are checked
Whether paper needs replacing:
1., if desired replace, then replace water suction block in nutrition liquid cartridge and waste liquid absorption cell and feed flow guiding filter paper
Filter paper is guided with waste liquid；
2., if no replacement is required, then follow the steps (7)；
(7), biological tissue or cell are observed online by microscope.
The online culture apparatus of biological tissue of the invention and observation method have the advantage that
(1), the present invention provides one kind and makes full use of existing equipment at hand, and low cost, flexibly carries out biological tissue at simplicity
Or the method that cell is cultivated under high magnification microscope and observed in real time, existing microscopical enlargement ratio, nothing are not influenced
Microscope need to be transformed, size can accomplish the size no more than existing concave slide；
(2), concave slide, coverslip, feed flow guiding filter paper and waste liquid guiding filter paper can use current material, nutrient solution feed flow
The bottom of box and waste liquid absorption cell offers feed flow open-work respectively and waste liquid absorbs open-work, then guides filter paper and waste liquid by feed flow
It guides filter paper connection culture chamber and forms flowing link, the water suction block in nutrient solution liquid cartridge and waste liquid absorption cell can select degreasing
Cotton etc., associated materials, which obtain, to be extremely easy, therefore the configuration of the present invention is simple, easy to make and at low cost；
(3), due to using general concave slide and coverslip, thickness not will increase, and microscope is without transformation, also not shadow
Ring microscope magnification；
(4), only need to adjust feed flow guiding filter paper and waste liquid guiding filter paper width achieve that for draining flow control,
It is easy easily to realize；
(5), feed flow guiding filter paper and waste liquid guiding filter paper are to be realized for drain using capillary water absorption principle without outside
Power；
(6), the effect being filtered to nutrient solution and waste liquid may be implemented in feed flow guiding filter paper and waste liquid guiding filter paper；
(7), nutrient solution liquid cartridge and waste liquid absorption cell as needed can with flexible configuration, for natural evaporation seldom or
Only requiring supplementation with the application scenarios such as tissue, the cell of a small amount of nutrient solution only can not need waste liquid absorption with nutrient solution liquid cartridge
Box；It only needs to seal can sealing coverslip for culture for not needing nutrient solution, is not necessarily to nutrient solution supply and discharge container.
Therefore the present invention has the spies such as reasonable, simple, easy to process, small in size, the easy to use, multi-purpose content of structure of design
Point, thus, it has good value for applications.
Detailed description of the invention
The following further describes the present invention with reference to the drawings.
Attached drawing 1 is the schematic perspective view of the online culture apparatus of biological tissue；
Attached drawing 2 is the top view of the online culture apparatus of biological tissue of attached drawing 1；
Attached drawing 3 is the structural schematic diagram of concave slide in attached drawing 1；
Attached drawing 4 is the structural schematic diagram of nutrient solution liquid cartridge；
Attached drawing 5 is the structural schematic diagram of waste liquid absorption cell；
Attached drawing 6 is that the nutrient solution of the online culture apparatus of biological tissue flows to schematic diagram；
Attached drawing 7 is the flow diagram that observation method is cultivated online by biological tissue.
In figure: 1, concave slide, 2, coverslip, 3, feed flow guiding filter paper, 4, nutrient solution liquid cartridge, 5, waste liquid absorption cell, 6,
Absorb water block, 7, culture chamber inlet, 8, culture chamber leakage fluid dram, 9, sealant, 10, culture chamber, 11, waste liquid guiding filter paper, 12, confession
Liquid open-work, 13, waste liquid absorption open-work, 14, groove.
Specific embodiment
Referring to Figure of description and specific embodiment to the online culture apparatus of a kind of biological tissue of the invention and watcher
Method is described in detail below.
In the description of the present invention, it is to be understood that, term " on ", "lower", "front", "rear", "left", "right", " perpendicular
Directly " orientation or positional relationship of the instructions such as "horizontal", "top", "bottom", "inner", "outside" is orientation based on the figure or position
Relationship is merely for convenience of description of the present invention and simplification of the description.Rather than the device or element of indication or suggestion meaning must have
There is specific orientation, be constructed and operated in a specific orientation, therefore should not be understood as limitation of the invention.In addition, term " the
One ", " second ", " third " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
To be mechanical connection, it is also possible to be electrically connected；It can be and be directly connected to, can also can be indirectly connected through an intermediary
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood at this with concrete condition
Concrete meaning in invention.
Embodiment 1:
As shown in figure 1 and 2, the online culture apparatus of biological tissue of the invention, structure include concave slide 1 and coverslip
2, as shown in Fig. 3, the disc-shaped structure of groove 14 is equipped with centered on concave slide 1, coverslip 2 is located on concave slide 1 and is located at
The top of 1 groove 14 of concave slide, 1 groove 14 of concave slide form culture chamber 10 with coverslip 2；It is equipped on concave slide 1 for drain
System, includes feed flow subsystem and drain subsystem for drainage system, and feed flow subsystem and drain subsystem are located at culture
It the two sides of chamber 10 and is connected to respectively with culture chamber 10.Culture chamber inlet 7 and training are respectively equipped at the opposite two sides of culture chamber 10
Chamber leakage fluid dram 8 is supported, feed flow subsystem is connected to culture chamber 10 by culture chamber inlet 7, and drain subsystem passes through culture chamber drain
Mouth 8 is connected to culture chambers 10, and culture chamber inlet 7 and culture chamber leakage fluid dram 8 are respectively positioned on coverslip 2 and 1 junction of concave slide.Lid glass
The junction of piece 2 and concave slide 1 is tightly connected using sealant 9, only retains culture chamber inlet 7 and culture chamber leakage fluid dram 8, control
Controlling flow direction is formed for apocenosis passage, by preventing liquid to other direction diffusions, it is ensured that the leakproofness of culture chamber 10.
Feed flow subsystem includes nutrient solution liquid cartridge 4, and as shown in Fig. 4,4 bottom of nutrient solution liquid cartridge offers feed flow open-work 12, is supplied
Feed flow guiding filter paper 3 is installed, feed flow guiding 3 one end of filter paper is located at feed flow open-work between liquid open-work 12 and culture chamber inlet 7
At 12, feed flow guiding 3 other end of filter paper is located at culture chamber inlet 7, and feed flow guiding filter paper 3 is for being connected to nutrient solution liquid cartridge
4 and culture chamber 10.Feed flow guiding filter paper 3 is located on concave slide 1 and between nutrient solution liquid cartridge 4 and coverslip 2.Drain
System includes waste liquid absorption cell 5, and as shown in Fig. 5, the bottom of waste liquid absorption cell 5 offers waste liquid and absorbs open-work 13, and waste liquid is inhaled
It receives and waste liquid guiding filter paper 11 is installed between open-work 13 and culture solution leakage fluid dram 8, waste liquid guiding 11 one end of filter paper is located at waste liquid suction
It receives at open-work 13, waste liquid guiding 11 other end of filter paper is located at culture solution leakage fluid dram 8, and waste liquid guiding filter paper 11 is for being connected to waste liquid suction
Receive box 5 and culture chamber 10.Waste liquid guiding filter paper 11 is located on concave slide 1 and between waste liquid absorption cell 5 and coverslip 2.Battalion
Water suction block 6 is separately installed in nutrient solution liquid cartridge 4 and waste liquid absorption cell 5, water suction block 6 is using rectangular knot made of water-absorbent material
Structure body.
As shown in Fig. 6, as follows for drainage system supply and discharge process: the water suction block 6 in nutrient solution liquid cartridge 4 absorbs addition
Nutrient solution, nutrient solution flow to right side feed flow by the feed flow open-work 12 of 4 bottom of nutrient solution liquid cartridge and guide filter paper 3, right side feed flow
Nutrient solution is supplied to culture chamber 10 by 2 right side culture chamber inlet 7 of coverslip by guiding filter paper 3, the waste liquid in culture chamber 10
Left side waste liquid is flowed to by the culture chamber leakage fluid dram 8 at left side coverslip edge and guides filter paper 11, and left side waste liquid guides filter paper 11 will
Waste liquid absorbs open-work 13 by the waste liquid of 5 bottom of waste liquid absorption cell and is transported in the absorption block 6 in waste liquid absorption cell 5, completes one
A feed flow and drain circulation.
Embodiment 2:
As shown in Fig. 7, observation method is cultivated online by biological tissue of the invention, and the method steps are as follows:
(1), it builds culture chamber 10 and determinand is put into culture chamber 10: biological tissues under test or cell are put into recessed glass
In the groove 14 of piece 1, appropriate solution and covered 2 are instilled；
(2), closed culture environment is built: after instilling the air that liquid is discharged in culture chamber 10 into culture chamber 10,
Lid fragmentation 2 and concave slide 1 are sealed by sealant 9 and reserve culture chamber inlet 7 and culture chamber leakage fluid dram 8；
(3), build for drainage system: the online culture apparatus of biological tissue as claimed in one of claims 1-8 is built
For drainage system；
(4), it adjusts for draining flow: filter paper 3 being guided by feed flow and waste liquid guides the width and length adjustment of filter paper 11
Required draining flow；
(5), it injects nutrient solution and waste collection: injecting nutrient solution in the water suction block 6 in nutrient solution liquid cartridge 4, adjust
Feed flow guiding filter paper 3 and waste liquid guiding filter paper 11 make entirely to form flowing link for drainage system；
(6), check that nutrition liquid cartridge 4 and water suction block 6 in waste liquid absorption cell 5 and feed flow guiding filter paper 3 and waste liquid are led
Draw whether filter paper 11 needs replacing:
1., if desired replace, then replace water suction block 6 in nutrition liquid cartridge 4 and waste liquid absorption cell 5 and feed flow guiding filter
Paper 3 and waste liquid guide filter paper 11；
2., if no replacement is required, then follow the steps (7)；
(7), biological tissue or cell are observed online by microscope.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (9)
1. a kind of online culture apparatus of biological tissue, which is characterized in that including concave slide and coverslip, be equipped with centered on concave slide
The top on concave slide and being located at concave slide groove, concave slide groove and lid glass is arranged in the disc-shaped structure of groove, coverslip
Piece forms culture chamber；
It is provided on concave slide for drainage system, includes feed flow subsystem and drain subsystem, feed flow subsystem for drainage system
The two sides of culture chamber are located at drain subsystem and feed flow subsystem and drain subsystem are connected to culture chamber respectively.
2. the online culture apparatus of biological tissue according to claim 1, which is characterized in that the opposite two sides of the culture chamber
Place is respectively arranged with culture chamber inlet and culture chamber leakage fluid dram, and feed flow subsystem is connected to culture chamber by culture chamber inlet,
Drain subsystem is connected to culture chamber by culture chamber leakage fluid dram, culture chamber inlet and culture chamber leakage fluid dram be respectively positioned on coverslip with
Concave slide junction.
3. the online culture apparatus of biological tissue according to claim 2, which is characterized in that the coverslip and concave slide
Junction is tightly connected using sealant.
4. the online culture apparatus of biological tissue according to claim 1 or 2 or 3, which is characterized in that the feed flow subsystem
Including nutrient solution liquid cartridge, nutrient solution liquid cartridge bottom offers feed flow open-work, sets between feed flow open-work and culture chamber inlet
It is equipped with feed flow guiding filter paper, feed flow guiding filter paper one end is located at feed flow through-hole position, and the feed flow guiding filter paper other end is located at culture chamber
At inlet, feed flow guiding filter paper is for being connected to nutrient solution liquid cartridge and culture chamber.
5. the online culture apparatus of biological tissue according to claim 4, which is characterized in that the feed flow guiding filter paper setting
On concave slide and between nutrient solution liquid cartridge and coverslip.
6. the online culture apparatus of biological tissue according to claim 5, which is characterized in that the drain subsystem includes useless
Liquid absorption cell, the bottom of waste liquid absorption cell offer waste liquid and absorb open-work, and waste liquid absorbs to be set between open-work and culture solution leakage fluid dram
It is equipped with waste liquid guiding filter paper, waste liquid guiding filter paper one end is located at waste liquid and absorbs through-hole position, and waste liquid guides the filter paper other end and is located at training
Nutrient solution leakage fluid dram, waste liquid guiding filter paper is for being connected to waste liquid absorption cell and culture chamber.
7. the online culture apparatus of biological tissue according to claim 6, which is characterized in that the waste liquid guiding filter paper setting
On concave slide and between waste liquid absorption cell and coverslip.
8. the online culture apparatus of biological tissue according to claim 7, which is characterized in that the nutrient solution liquid cartridge and useless
Water suction block is respectively arranged in liquid absorption cell, water suction block is using square structure body made of water-absorbent material.
9. observation method is cultivated online by a kind of biological tissue, which is characterized in that the method steps are as follows:
(1), it builds culture chamber and determinand is put into culture chamber: biological tissues under test or cell are put into the groove of concave slide
In, instill appropriate solution and covered；
(2), closed culture environment is built: after instilling the air that liquid is discharged in culture chamber into culture chamber, by lid fragmentation
It is sealed with concave slide by sealant and reserves culture chamber inlet and culture chamber leakage fluid dram；
(3), build for drainage system: the online culture apparatus of biological tissue as claimed in one of claims 1-8 builds supply and discharge
Liquid system；
(4), it adjusts for draining flow: by row needed for the width and length adjustment of feed flow guiding filter paper and waste liquid guiding filter paper
Flow quantity；
(5), it injects nutrient solution and waste collection: injecting nutrient solution in the water suction block in nutrient solution liquid cartridge, adjustment feed flow is led
Drawing filter paper and waste liquid guiding filter paper makes entirely to form flowing link for drainage system；
(6), nutrition liquid cartridge and water suction block in waste liquid absorption cell and feed flow guiding filter paper are checked and waste liquid guiding filter paper is
It is no to need replacing:
1., if desired replace, then replace water suction block in nutrition liquid cartridge and waste liquid absorption cell and feed flow guiding filter paper and useless
Liquid guides filter paper；
2., if no replacement is required, then follow the steps (7)；
(7), biological tissue or cell are observed online by microscope.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910174562.0A CN109735449A (en) | 2019-03-08 | 2019-03-08 | A kind of online culture apparatus of biological tissue and observation method |
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| CN201910174562.0A CN109735449A (en) | 2019-03-08 | 2019-03-08 | A kind of online culture apparatus of biological tissue and observation method |
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