CN109734784B - SlDALR1基因在增强番茄细菌性叶斑病抗性中的应用 - Google Patents
SlDALR1基因在增强番茄细菌性叶斑病抗性中的应用 Download PDFInfo
- Publication number
- CN109734784B CN109734784B CN201910085289.4A CN201910085289A CN109734784B CN 109734784 B CN109734784 B CN 109734784B CN 201910085289 A CN201910085289 A CN 201910085289A CN 109734784 B CN109734784 B CN 109734784B
- Authority
- CN
- China
- Prior art keywords
- sldalr1
- leu
- gene
- tomato
- ser
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 235000007688 Lycopersicon esculentum Nutrition 0.000 title claims abstract description 89
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 89
- 230000001580 bacterial effect Effects 0.000 title claims abstract description 63
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 12
- 240000003768 Solanum lycopersicum Species 0.000 title description 92
- 239000002773 nucleotide Substances 0.000 claims abstract description 11
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 11
- 241000227653 Lycopersicon Species 0.000 claims abstract description 9
- 108700026244 Open Reading Frames Proteins 0.000 claims abstract description 8
- 230000002018 overexpression Effects 0.000 claims description 29
- 241000589158 Agrobacterium Species 0.000 claims description 25
- 239000013598 vector Substances 0.000 claims description 25
- 241000894006 Bacteria Species 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 13
- 101710140185 Receptor-like protein kinase Proteins 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 9
- 230000009261 transgenic effect Effects 0.000 claims description 7
- 238000010353 genetic engineering Methods 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 230000009466 transformation Effects 0.000 claims description 5
- 241000589155 Agrobacterium tumefaciens Species 0.000 claims description 4
- 230000001404 mediated effect Effects 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 2
- 230000001488 breeding effect Effects 0.000 claims description 2
- 238000011144 upstream manufacturing Methods 0.000 claims description 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 description 40
- 239000007788 liquid Substances 0.000 description 34
- 201000010099 disease Diseases 0.000 description 27
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 27
- 102000004169 proteins and genes Human genes 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 16
- 244000052616 bacterial pathogen Species 0.000 description 13
- 108020004414 DNA Proteins 0.000 description 12
- 239000001963 growth medium Substances 0.000 description 12
- 210000000170 cell membrane Anatomy 0.000 description 10
- 230000014509 gene expression Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 102000001253 Protein Kinase Human genes 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 230000001717 pathogenic effect Effects 0.000 description 8
- 108060006633 protein kinase Proteins 0.000 description 8
- 108091000080 Phosphotransferase Proteins 0.000 description 7
- 238000010378 bimolecular fluorescence complementation Methods 0.000 description 7
- 239000005090 green fluorescent protein Substances 0.000 description 7
- 244000052769 pathogen Species 0.000 description 7
- 102000020233 phosphotransferase Human genes 0.000 description 7
- 108091005957 yellow fluorescent proteins Proteins 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 241000207746 Nicotiana benthamiana Species 0.000 description 6
- 235000002560 Solanum lycopersicum Nutrition 0.000 description 6
- 229960005091 chloramphenicol Drugs 0.000 description 6
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 6
- 238000003208 gene overexpression Methods 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 description 5
- 239000002299 complementary DNA Substances 0.000 description 5
- 238000005520 cutting process Methods 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 239000013604 expression vector Substances 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000003752 polymerase chain reaction Methods 0.000 description 5
- 238000001179 sorption measurement Methods 0.000 description 5
- 238000012795 verification Methods 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- 206010020649 Hyperkeratosis Diseases 0.000 description 4
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 4
- OJOBTAOGJIWAGB-UHFFFAOYSA-N acetosyringone Chemical compound COC1=CC(C(C)=O)=CC(OC)=C1O OJOBTAOGJIWAGB-UHFFFAOYSA-N 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- 238000010276 construction Methods 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 4
- VPZXBVLAVMBEQI-UHFFFAOYSA-N glycyl-DL-alpha-alanine Natural products OC(=O)C(C)NC(=O)CN VPZXBVLAVMBEQI-UHFFFAOYSA-N 0.000 description 4
- 208000015181 infectious disease Diseases 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 230000000474 nursing effect Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 108020003175 receptors Proteins 0.000 description 4
- 102000005962 receptors Human genes 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000010839 reverse transcription Methods 0.000 description 4
- 239000008223 sterile water Substances 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 208000024891 symptom Diseases 0.000 description 4
- 108010061238 threonyl-glycine Proteins 0.000 description 4
- 239000002699 waste material Substances 0.000 description 4
- 101001053892 Arabidopsis thaliana LRR receptor-like serine/threonine-protein kinase EFR Proteins 0.000 description 3
- YXVAESUIQFDBHN-SRVKXCTJSA-N Asn-Phe-Ser Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(O)=O YXVAESUIQFDBHN-SRVKXCTJSA-N 0.000 description 3
- YNCHFVRXEQFPBY-BQBZGAKWSA-N Asp-Gly-Arg Chemical compound OC(=O)C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCCN=C(N)N YNCHFVRXEQFPBY-BQBZGAKWSA-N 0.000 description 3
- UMHUHHJMEXNSIV-CIUDSAMLSA-N Asp-Leu-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC(O)=O UMHUHHJMEXNSIV-CIUDSAMLSA-N 0.000 description 3
- 208000035240 Disease Resistance Diseases 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 3
- OJMMVQQUTAEWLP-UHFFFAOYSA-N Lincomycin Natural products CN1CC(CCC)CC1C(=O)NC(C(C)O)C1C(O)C(O)C(O)C(SC)O1 OJMMVQQUTAEWLP-UHFFFAOYSA-N 0.000 description 3
- 108010079364 N-glycylalanine Proteins 0.000 description 3
- 241000208125 Nicotiana Species 0.000 description 3
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 3
- 102000008153 Peptide Elongation Factor Tu Human genes 0.000 description 3
- 108010049977 Peptide Elongation Factor Tu Proteins 0.000 description 3
- 239000008346 aqueous phase Substances 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 238000010790 dilution Methods 0.000 description 3
- 239000012895 dilution Substances 0.000 description 3
- 230000028993 immune response Effects 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 230000003834 intracellular effect Effects 0.000 description 3
- 229960005287 lincomycin Drugs 0.000 description 3
- OJMMVQQUTAEWLP-KIDUDLJLSA-N lincomycin Chemical compound CN1C[C@H](CCC)C[C@H]1C(=O)N[C@H]([C@@H](C)O)[C@@H]1[C@H](O)[C@H](O)[C@@H](O)[C@@H](SC)O1 OJMMVQQUTAEWLP-KIDUDLJLSA-N 0.000 description 3
- 230000004807 localization Effects 0.000 description 3
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L magnesium chloride Substances [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 108010089193 pattern recognition receptors Proteins 0.000 description 3
- 108010051242 phenylalanylserine Proteins 0.000 description 3
- 230000008121 plant development Effects 0.000 description 3
- 230000006916 protein interaction Effects 0.000 description 3
- 210000003491 skin Anatomy 0.000 description 3
- 230000004960 subcellular localization Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- HQJKCXHQNUCKMY-GHCJXIJMSA-N Ala-Ile-Asp Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)O)NC(=O)[C@H](C)N HQJKCXHQNUCKMY-GHCJXIJMSA-N 0.000 description 2
- 241000219195 Arabidopsis thaliana Species 0.000 description 2
- PNHQRQTVBRDIEF-CIUDSAMLSA-N Asn-Leu-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(=O)N)N PNHQRQTVBRDIEF-CIUDSAMLSA-N 0.000 description 2
- 102100032305 Bcl-2 homologous antagonist/killer Human genes 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 2
- 229930182566 Gentamicin Natural products 0.000 description 2
- XFAUJGNLHIGXET-AVGNSLFASA-N Gln-Leu-Leu Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O XFAUJGNLHIGXET-AVGNSLFASA-N 0.000 description 2
- STVHDEHTKFXBJQ-LAEOZQHASA-N Gly-Glu-Ile Chemical compound [H]NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O STVHDEHTKFXBJQ-LAEOZQHASA-N 0.000 description 2
- GDOZQTNZPCUARW-YFKPBYRVSA-N Gly-Gly-Glu Chemical compound NCC(=O)NCC(=O)N[C@H](C(O)=O)CCC(O)=O GDOZQTNZPCUARW-YFKPBYRVSA-N 0.000 description 2
- 101000798320 Homo sapiens Bcl-2 homologous antagonist/killer Proteins 0.000 description 2
- DLCOFDAHNMMQPP-SRVKXCTJSA-N Leu-Asp-Leu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O DLCOFDAHNMMQPP-SRVKXCTJSA-N 0.000 description 2
- HFBCHNRFRYLZNV-GUBZILKMSA-N Leu-Glu-Asp Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O HFBCHNRFRYLZNV-GUBZILKMSA-N 0.000 description 2
- HVHRPWQEQHIQJF-AVGNSLFASA-N Leu-Lys-Glu Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(O)=O)C(O)=O HVHRPWQEQHIQJF-AVGNSLFASA-N 0.000 description 2
- IDGZVZJLYFTXSL-DCAQKATOSA-N Leu-Ser-Arg Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCCN=C(N)N IDGZVZJLYFTXSL-DCAQKATOSA-N 0.000 description 2
- IWMJFLJQHIDZQW-KKUMJFAQSA-N Leu-Ser-Phe Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 IWMJFLJQHIDZQW-KKUMJFAQSA-N 0.000 description 2
- SITLTJHOQZFJGG-UHFFFAOYSA-N N-L-alpha-glutamyl-L-valine Natural products CC(C)C(C(O)=O)NC(=O)C(N)CCC(O)=O SITLTJHOQZFJGG-UHFFFAOYSA-N 0.000 description 2
- XMBSYZWANAQXEV-UHFFFAOYSA-N N-alpha-L-glutamyl-L-phenylalanine Natural products OC(=O)CCC(N)C(=O)NC(C(O)=O)CC1=CC=CC=C1 XMBSYZWANAQXEV-UHFFFAOYSA-N 0.000 description 2
- KZNQNBZMBZJQJO-UHFFFAOYSA-N N-glycyl-L-proline Natural products NCC(=O)N1CCCC1C(O)=O KZNQNBZMBZJQJO-UHFFFAOYSA-N 0.000 description 2
- XYSXOCIWCPFOCG-IHRRRGAJSA-N Pro-Leu-Leu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O XYSXOCIWCPFOCG-IHRRRGAJSA-N 0.000 description 2
- FNGOXVQBBCMFKV-CIUDSAMLSA-N Pro-Ser-Glu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(O)=O FNGOXVQBBCMFKV-CIUDSAMLSA-N 0.000 description 2
- 102000009516 Protein Serine-Threonine Kinases Human genes 0.000 description 2
- 108010009341 Protein Serine-Threonine Kinases Proteins 0.000 description 2
- MOINZPRHJGTCHZ-MMWGEVLESA-N Ser-Ile-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CO)N MOINZPRHJGTCHZ-MMWGEVLESA-N 0.000 description 2
- 241000208292 Solanaceae Species 0.000 description 2
- NQQMWWVVGIXUOX-SVSWQMSJSA-N Thr-Ser-Ile Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O NQQMWWVVGIXUOX-SVSWQMSJSA-N 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 108010062796 arginyllysine Proteins 0.000 description 2
- 108010047857 aspartylglycine Proteins 0.000 description 2
- 108010092854 aspartyllysine Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 238000004925 denaturation Methods 0.000 description 2
- 230000036425 denaturation Effects 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 229960002518 gentamicin Drugs 0.000 description 2
- 108010010147 glycylglutamine Proteins 0.000 description 2
- 108010050848 glycylleucine Proteins 0.000 description 2
- 108010037850 glycylvaline Proteins 0.000 description 2
- 108010036413 histidylglycine Proteins 0.000 description 2
- 108010034529 leucyl-lysine Proteins 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 108010054155 lysyllysine Proteins 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 102000007863 pattern recognition receptors Human genes 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- JQXXHWHPUNPDRT-WLSIYKJHSA-N rifampicin Chemical compound O([C@](C1=O)(C)O/C=C/[C@@H]([C@H]([C@@H](OC(C)=O)[C@H](C)[C@H](O)[C@H](C)[C@@H](O)[C@@H](C)\C=C\C=C(C)/C(=O)NC=2C(O)=C3C([O-])=C4C)C)OC)C4=C1C3=C(O)C=2\C=N\N1CC[NH+](C)CC1 JQXXHWHPUNPDRT-WLSIYKJHSA-N 0.000 description 2
- 229960001225 rifampicin Drugs 0.000 description 2
- 239000012883 rooting culture medium Substances 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 210000003462 vein Anatomy 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 108010027345 wheylin-1 peptide Proteins 0.000 description 2
- AXFMEGAFCUULFV-BLFANLJRSA-N (2s)-2-[[(2s)-1-[(2s,3r)-2-amino-3-methylpentanoyl]pyrrolidine-2-carbonyl]amino]pentanedioic acid Chemical compound CC[C@@H](C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(O)=O AXFMEGAFCUULFV-BLFANLJRSA-N 0.000 description 1
- DHSSDEDRBUKTQY-UHFFFAOYSA-N 6-prop-2-enyl-4,5,7,8-tetrahydrothiazolo[4,5-d]azepin-2-amine Chemical compound C1CN(CC=C)CCC2=C1N=C(N)S2 DHSSDEDRBUKTQY-UHFFFAOYSA-N 0.000 description 1
- 102100032814 ATP-dependent zinc metalloprotease YME1L1 Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- YBPLKDWJFYCZSV-ZLUOBGJFSA-N Ala-Asn-Cys Chemical compound C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CS)C(=O)O)N YBPLKDWJFYCZSV-ZLUOBGJFSA-N 0.000 description 1
- STACJSVFHSEZJV-GHCJXIJMSA-N Ala-Asn-Ile Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O STACJSVFHSEZJV-GHCJXIJMSA-N 0.000 description 1
- YSMPVONNIWLJML-FXQIFTODSA-N Ala-Asp-Pro Chemical compound C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(O)=O YSMPVONNIWLJML-FXQIFTODSA-N 0.000 description 1
- WKOBSJOZRJJVRZ-FXQIFTODSA-N Ala-Glu-Glu Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O WKOBSJOZRJJVRZ-FXQIFTODSA-N 0.000 description 1
- UHMQKOBNPRAZGB-CIUDSAMLSA-N Ala-Glu-Met Chemical compound C[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCSC)C(=O)O)N UHMQKOBNPRAZGB-CIUDSAMLSA-N 0.000 description 1
- BLIMFWGRQKRCGT-YUMQZZPRSA-N Ala-Gly-Lys Chemical compound C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCCCN BLIMFWGRQKRCGT-YUMQZZPRSA-N 0.000 description 1
- SOBIAADAMRHGKH-CIUDSAMLSA-N Ala-Leu-Ser Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O SOBIAADAMRHGKH-CIUDSAMLSA-N 0.000 description 1
- MDNAVFBZPROEHO-DCAQKATOSA-N Ala-Lys-Val Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C(C)C)C(O)=O MDNAVFBZPROEHO-DCAQKATOSA-N 0.000 description 1
- MDNAVFBZPROEHO-UHFFFAOYSA-N Ala-Lys-Val Natural products CC(C)C(C(O)=O)NC(=O)C(NC(=O)C(C)N)CCCCN MDNAVFBZPROEHO-UHFFFAOYSA-N 0.000 description 1
- DWYROCSXOOMOEU-CIUDSAMLSA-N Ala-Met-Glu Chemical compound C[C@@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N DWYROCSXOOMOEU-CIUDSAMLSA-N 0.000 description 1
- VRTOMXFZHGWHIJ-KZVJFYERSA-N Ala-Thr-Arg Chemical compound [H]N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O VRTOMXFZHGWHIJ-KZVJFYERSA-N 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- 108700022755 Arabidopsis EFR Proteins 0.000 description 1
- 101100218485 Arabidopsis thaliana BAK1 gene Proteins 0.000 description 1
- PEFFAAKJGBZBKL-NAKRPEOUSA-N Arg-Ala-Ile Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O PEFFAAKJGBZBKL-NAKRPEOUSA-N 0.000 description 1
- CYXCAHZVPFREJD-LURJTMIESA-N Arg-Gly-Gly Chemical compound NC(=N)NCCC[C@H](N)C(=O)NCC(=O)NCC(O)=O CYXCAHZVPFREJD-LURJTMIESA-N 0.000 description 1
- WVNFNPGXYADPPO-BQBZGAKWSA-N Arg-Gly-Ser Chemical compound NC(N)=NCCC[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O WVNFNPGXYADPPO-BQBZGAKWSA-N 0.000 description 1
- BMNVSPMWMICFRV-DCAQKATOSA-N Arg-His-Asp Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CC(O)=O)C(O)=O)CC1=CN=CN1 BMNVSPMWMICFRV-DCAQKATOSA-N 0.000 description 1
- FLYANDHDFRGGTM-PYJNHQTQSA-N Arg-Ile-His Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N FLYANDHDFRGGTM-PYJNHQTQSA-N 0.000 description 1
- NMRHDSAOIURTNT-RWMBFGLXSA-N Arg-Leu-Pro Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N NMRHDSAOIURTNT-RWMBFGLXSA-N 0.000 description 1
- MNBHKGYCLBUIBC-UFYCRDLUSA-N Arg-Phe-Phe Chemical compound C([C@H](NC(=O)[C@H](CCCNC(N)=N)N)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 MNBHKGYCLBUIBC-UFYCRDLUSA-N 0.000 description 1
- WTUZDHWWGUQEKN-SRVKXCTJSA-N Arg-Val-Met Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCSC)C(O)=O WTUZDHWWGUQEKN-SRVKXCTJSA-N 0.000 description 1
- QLSRIZIDQXDQHK-RCWTZXSCSA-N Arg-Val-Thr Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O QLSRIZIDQXDQHK-RCWTZXSCSA-N 0.000 description 1
- GXMSVVBIAMWMKO-BQBZGAKWSA-N Asn-Arg-Gly Chemical compound NC(=O)C[C@H](N)C(=O)N[C@H](C(=O)NCC(O)=O)CCCN=C(N)N GXMSVVBIAMWMKO-BQBZGAKWSA-N 0.000 description 1
- RCENDENBBJFJHZ-ACZMJKKPSA-N Asn-Asn-Gln Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O RCENDENBBJFJHZ-ACZMJKKPSA-N 0.000 description 1
- NVGWESORMHFISY-SRVKXCTJSA-N Asn-Asn-Phe Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O NVGWESORMHFISY-SRVKXCTJSA-N 0.000 description 1
- QHBMKQWOIYJYMI-BYULHYEWSA-N Asn-Asn-Val Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(O)=O QHBMKQWOIYJYMI-BYULHYEWSA-N 0.000 description 1
- WVCJSDCHTUTONA-FXQIFTODSA-N Asn-Asp-Arg Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O WVCJSDCHTUTONA-FXQIFTODSA-N 0.000 description 1
- SPIPSJXLZVTXJL-ZLUOBGJFSA-N Asn-Cys-Ser Chemical compound NC(=O)C[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(O)=O SPIPSJXLZVTXJL-ZLUOBGJFSA-N 0.000 description 1
- QPTAGIPWARILES-AVGNSLFASA-N Asn-Gln-Phe Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O QPTAGIPWARILES-AVGNSLFASA-N 0.000 description 1
- COUZKSSMBFADSB-AVGNSLFASA-N Asn-Glu-Phe Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)N)N COUZKSSMBFADSB-AVGNSLFASA-N 0.000 description 1
- JRCASHGTXZYSPW-XIRDDKMYSA-N Asn-His-Trp Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)O)NC(=O)[C@H](CC3=CN=CN3)NC(=O)[C@H](CC(=O)N)N JRCASHGTXZYSPW-XIRDDKMYSA-N 0.000 description 1
- YYSYDIYQTUPNQQ-SXTJYALSSA-N Asn-Ile-Ile Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O YYSYDIYQTUPNQQ-SXTJYALSSA-N 0.000 description 1
- SEKBHZJLARBNPB-GHCJXIJMSA-N Asn-Ile-Ser Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CO)C(O)=O SEKBHZJLARBNPB-GHCJXIJMSA-N 0.000 description 1
- BXUHCIXDSWRSBS-CIUDSAMLSA-N Asn-Leu-Asp Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O BXUHCIXDSWRSBS-CIUDSAMLSA-N 0.000 description 1
- YVXRYLVELQYAEQ-SRVKXCTJSA-N Asn-Leu-Lys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC(=O)N)N YVXRYLVELQYAEQ-SRVKXCTJSA-N 0.000 description 1
- DJIMLSXHXKWADV-CIUDSAMLSA-N Asn-Leu-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC(N)=O DJIMLSXHXKWADV-CIUDSAMLSA-N 0.000 description 1
- FHETWELNCBMRMG-HJGDQZAQSA-N Asn-Leu-Thr Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O FHETWELNCBMRMG-HJGDQZAQSA-N 0.000 description 1
- NCFJQJRLQJEECD-NHCYSSNCSA-N Asn-Leu-Val Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(O)=O NCFJQJRLQJEECD-NHCYSSNCSA-N 0.000 description 1
- ORJQQZIXTOYGGH-SRVKXCTJSA-N Asn-Lys-Leu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(O)=O ORJQQZIXTOYGGH-SRVKXCTJSA-N 0.000 description 1
- WXVGISRWSYGEDK-KKUMJFAQSA-N Asn-Lys-Phe Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(=O)N)N WXVGISRWSYGEDK-KKUMJFAQSA-N 0.000 description 1
- BKFXFUPYETWGGA-XVSYOHENSA-N Asn-Phe-Thr Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)O)C(O)=O BKFXFUPYETWGGA-XVSYOHENSA-N 0.000 description 1
- IDUUACUJKUXKKD-VEVYYDQMSA-N Asn-Pro-Thr Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(O)=O IDUUACUJKUXKKD-VEVYYDQMSA-N 0.000 description 1
- VHQSGALUSWIYOD-QXEWZRGKSA-N Asn-Pro-Val Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(O)=O VHQSGALUSWIYOD-QXEWZRGKSA-N 0.000 description 1
- MKJBPDLENBUHQU-CIUDSAMLSA-N Asn-Ser-Leu Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O MKJBPDLENBUHQU-CIUDSAMLSA-N 0.000 description 1
- VLDRQOHCMKCXLY-SRVKXCTJSA-N Asn-Ser-Phe Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O VLDRQOHCMKCXLY-SRVKXCTJSA-N 0.000 description 1
- XLDMSQYOYXINSZ-QXEWZRGKSA-N Asn-Val-Arg Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)NC(=O)[C@H](CC(=O)N)N XLDMSQYOYXINSZ-QXEWZRGKSA-N 0.000 description 1
- NYLBGYLHBDFRHL-VEVYYDQMSA-N Asp-Arg-Thr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(O)=O NYLBGYLHBDFRHL-VEVYYDQMSA-N 0.000 description 1
- VBVKSAFJPVXMFJ-CIUDSAMLSA-N Asp-Asn-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CC(=O)O)N VBVKSAFJPVXMFJ-CIUDSAMLSA-N 0.000 description 1
- PXLNPFOJZQMXAT-BYULHYEWSA-N Asp-Asp-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CC(O)=O PXLNPFOJZQMXAT-BYULHYEWSA-N 0.000 description 1
- HOBNTSHITVVNBN-ZPFDUUQYSA-N Asp-Ile-Leu Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)O)NC(=O)[C@H](CC(=O)O)N HOBNTSHITVVNBN-ZPFDUUQYSA-N 0.000 description 1
- LBOVBQONZJRWPV-YUMQZZPRSA-N Asp-Lys-Gly Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)NCC(O)=O LBOVBQONZJRWPV-YUMQZZPRSA-N 0.000 description 1
- HXVILZUZXFLVEN-DCAQKATOSA-N Asp-Met-Leu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(C)C)C(O)=O HXVILZUZXFLVEN-DCAQKATOSA-N 0.000 description 1
- DRCOAZZDQRCGGP-GHCJXIJMSA-N Asp-Ser-Ile Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O DRCOAZZDQRCGGP-GHCJXIJMSA-N 0.000 description 1
- JSNWZMFSLIWAHS-HJGDQZAQSA-N Asp-Thr-Leu Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)O)NC(=O)[C@H](CC(=O)O)N)O JSNWZMFSLIWAHS-HJGDQZAQSA-N 0.000 description 1
- VHUKCUHLFMRHOD-MELADBBJSA-N Asp-Tyr-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC2=CC=C(C=C2)O)NC(=O)[C@H](CC(=O)O)N)C(=O)O VHUKCUHLFMRHOD-MELADBBJSA-N 0.000 description 1
- XWKBWZXGNXTDKY-ZKWXMUAHSA-N Asp-Val-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C(C)C)NC(=O)[C@@H](N)CC(O)=O XWKBWZXGNXTDKY-ZKWXMUAHSA-N 0.000 description 1
- QPDUWAUSSWGJSB-NGZCFLSTSA-N Asp-Val-Pro Chemical compound CC(C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CC(=O)O)N QPDUWAUSSWGJSB-NGZCFLSTSA-N 0.000 description 1
- JGLWFWXGOINXEA-YDHLFZDLSA-N Asp-Val-Tyr Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 JGLWFWXGOINXEA-YDHLFZDLSA-N 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241000219193 Brassicaceae Species 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- LHLSSZYQFUNWRZ-NAKRPEOUSA-N Cys-Arg-Ile Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O LHLSSZYQFUNWRZ-NAKRPEOUSA-N 0.000 description 1
- BNRHLRWCERLRTQ-BPUTZDHNSA-N Cys-Arg-Trp Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CS)N BNRHLRWCERLRTQ-BPUTZDHNSA-N 0.000 description 1
- WVLZTXGTNGHPBO-SRVKXCTJSA-N Cys-Leu-Leu Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(O)=O WVLZTXGTNGHPBO-SRVKXCTJSA-N 0.000 description 1
- UIKLEGZPIOXFHJ-DLOVCJGASA-N Cys-Phe-Ala Chemical compound [H]N[C@@H](CS)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](C)C(O)=O UIKLEGZPIOXFHJ-DLOVCJGASA-N 0.000 description 1
- YNJBLTDKTMKEET-ZLUOBGJFSA-N Cys-Ser-Ser Chemical compound SC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O YNJBLTDKTMKEET-ZLUOBGJFSA-N 0.000 description 1
- NRVQLLDIJJEIIZ-VZFHVOOUSA-N Cys-Thr-Ala Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](C)C(=O)O)NC(=O)[C@H](CS)N)O NRVQLLDIJJEIIZ-VZFHVOOUSA-N 0.000 description 1
- 108010090461 DFG peptide Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 101150100333 FLS2 gene Proteins 0.000 description 1
- 108010040721 Flagellin Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- XXLBHPPXDUWYAG-XQXXSGGOSA-N Gln-Ala-Thr Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O XXLBHPPXDUWYAG-XQXXSGGOSA-N 0.000 description 1
- LZRMPXRYLLTAJX-GUBZILKMSA-N Gln-Arg-Glu Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(O)=O)C(O)=O LZRMPXRYLLTAJX-GUBZILKMSA-N 0.000 description 1
- YRWWJCDWLVXTHN-LAEOZQHASA-N Gln-Ile-Gly Chemical compound CC[C@H](C)[C@@H](C(=O)NCC(=O)O)NC(=O)[C@H](CCC(=O)N)N YRWWJCDWLVXTHN-LAEOZQHASA-N 0.000 description 1
- FYAULIGIFPPOAA-ZPFDUUQYSA-N Gln-Ile-Met Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCSC)C(O)=O FYAULIGIFPPOAA-ZPFDUUQYSA-N 0.000 description 1
- VUVKKXPCKILIBD-AVGNSLFASA-N Gln-Leu-His Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)NC(=O)[C@H](CCC(=O)N)N VUVKKXPCKILIBD-AVGNSLFASA-N 0.000 description 1
- SXGMGNZEHFORAV-IUCAKERBSA-N Gln-Lys-Gly Chemical compound C(CCN)C[C@@H](C(=O)NCC(=O)O)NC(=O)[C@H](CCC(=O)N)N SXGMGNZEHFORAV-IUCAKERBSA-N 0.000 description 1
- CVPXINNKRTZBMO-CIUDSAMLSA-N Glu-Arg-Asn Chemical compound C(C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](CCC(=O)O)N)CN=C(N)N CVPXINNKRTZBMO-CIUDSAMLSA-N 0.000 description 1
- RQNYYRHRKSVKAB-GUBZILKMSA-N Glu-Cys-Leu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(C)C)C(O)=O RQNYYRHRKSVKAB-GUBZILKMSA-N 0.000 description 1
- ITBHUUMCJJQUSC-LAEOZQHASA-N Glu-Ile-Gly Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(O)=O ITBHUUMCJJQUSC-LAEOZQHASA-N 0.000 description 1
- UGSVSNXPJJDJKL-SDDRHHMPSA-N Glu-Leu-Pro Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCC(=O)O)N UGSVSNXPJJDJKL-SDDRHHMPSA-N 0.000 description 1
- ILWHFUZZCFYSKT-AVGNSLFASA-N Glu-Lys-Leu Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(O)=O ILWHFUZZCFYSKT-AVGNSLFASA-N 0.000 description 1
- SOEPMWQCTJITPZ-SRVKXCTJSA-N Glu-Met-Lys Chemical compound CSCC[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CCC(=O)O)N SOEPMWQCTJITPZ-SRVKXCTJSA-N 0.000 description 1
- KJBGAZSLZAQDPV-KKUMJFAQSA-N Glu-Phe-Arg Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)NC(=O)[C@H](CCC(=O)O)N KJBGAZSLZAQDPV-KKUMJFAQSA-N 0.000 description 1
- GPSHCSTUYOQPAI-JHEQGTHGSA-N Glu-Thr-Gly Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(O)=O GPSHCSTUYOQPAI-JHEQGTHGSA-N 0.000 description 1
- UMZHHILWZBFPGL-LOKLDPHHSA-N Glu-Thr-Pro Chemical compound C[C@H]([C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCC(=O)O)N)O UMZHHILWZBFPGL-LOKLDPHHSA-N 0.000 description 1
- JRDYDYXZKFNNRQ-XPUUQOCRSA-N Gly-Ala-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)CN JRDYDYXZKFNNRQ-XPUUQOCRSA-N 0.000 description 1
- JPXNYFOHTHSREU-UWVGGRQHSA-N Gly-Arg-His Chemical compound C1=C(NC=N1)C[C@@H](C(=O)O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)CN JPXNYFOHTHSREU-UWVGGRQHSA-N 0.000 description 1
- OVSKVOOUFAKODB-UWVGGRQHSA-N Gly-Arg-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CCCN=C(N)N OVSKVOOUFAKODB-UWVGGRQHSA-N 0.000 description 1
- GGEJHJIXRBTJPD-BYPYZUCNSA-N Gly-Asn-Gly Chemical compound NCC(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O GGEJHJIXRBTJPD-BYPYZUCNSA-N 0.000 description 1
- BGVYNAQWHSTTSP-BYULHYEWSA-N Gly-Asn-Ile Chemical compound [H]NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O BGVYNAQWHSTTSP-BYULHYEWSA-N 0.000 description 1
- XCLCVBYNGXEVDU-WHFBIAKZSA-N Gly-Asn-Ser Chemical compound NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(O)=O XCLCVBYNGXEVDU-WHFBIAKZSA-N 0.000 description 1
- DHDOADIPGZTAHT-YUMQZZPRSA-N Gly-Glu-Arg Chemical compound NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@H](C(O)=O)CCCN=C(N)N DHDOADIPGZTAHT-YUMQZZPRSA-N 0.000 description 1
- UQJNXZSSGQIPIQ-FBCQKBJTSA-N Gly-Gly-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)CNC(=O)CN UQJNXZSSGQIPIQ-FBCQKBJTSA-N 0.000 description 1
- UTYGDAHJBBDPBA-BYULHYEWSA-N Gly-Ile-Asp Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)O)NC(=O)CN UTYGDAHJBBDPBA-BYULHYEWSA-N 0.000 description 1
- HKSNHPVETYYJBK-LAEOZQHASA-N Gly-Ile-Glu Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)O)NC(=O)CN HKSNHPVETYYJBK-LAEOZQHASA-N 0.000 description 1
- HMHRTKOWRUPPNU-RCOVLWMOSA-N Gly-Ile-Gly Chemical compound NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(O)=O HMHRTKOWRUPPNU-RCOVLWMOSA-N 0.000 description 1
- UESJMAMHDLEHGM-NHCYSSNCSA-N Gly-Ile-Leu Chemical compound NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(O)=O UESJMAMHDLEHGM-NHCYSSNCSA-N 0.000 description 1
- COVXELOAORHTND-LSJOCFKGSA-N Gly-Ile-Val Chemical compound NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C(C)C)C(O)=O COVXELOAORHTND-LSJOCFKGSA-N 0.000 description 1
- PAWIVEIWWYGBAM-YUMQZZPRSA-N Gly-Leu-Ala Chemical compound NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(O)=O PAWIVEIWWYGBAM-YUMQZZPRSA-N 0.000 description 1
- IUZGUFAJDBHQQV-YUMQZZPRSA-N Gly-Leu-Asn Chemical compound NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O IUZGUFAJDBHQQV-YUMQZZPRSA-N 0.000 description 1
- YTSVAIMKVLZUDU-YUMQZZPRSA-N Gly-Leu-Asp Chemical compound [H]NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(O)=O)C(O)=O YTSVAIMKVLZUDU-YUMQZZPRSA-N 0.000 description 1
- ULZCYBYDTUMHNF-IUCAKERBSA-N Gly-Leu-Glu Chemical compound NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O ULZCYBYDTUMHNF-IUCAKERBSA-N 0.000 description 1
- LHYJCVCQPWRMKZ-WEDXCCLWSA-N Gly-Leu-Thr Chemical compound [H]NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O LHYJCVCQPWRMKZ-WEDXCCLWSA-N 0.000 description 1
- NTBOEZICHOSJEE-YUMQZZPRSA-N Gly-Lys-Ser Chemical compound [H]NCC(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O NTBOEZICHOSJEE-YUMQZZPRSA-N 0.000 description 1
- WZSHYFGOLPXPLL-RYUDHWBXSA-N Gly-Phe-Glu Chemical compound NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(O)=O)C(O)=O WZSHYFGOLPXPLL-RYUDHWBXSA-N 0.000 description 1
- WNZOCXUOGVYYBJ-CDMKHQONSA-N Gly-Phe-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=CC=C1)NC(=O)CN)O WNZOCXUOGVYYBJ-CDMKHQONSA-N 0.000 description 1
- IXHQLZIWBCQBLQ-STQMWFEESA-N Gly-Pro-Phe Chemical compound NCC(=O)N1CCC[C@H]1C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 IXHQLZIWBCQBLQ-STQMWFEESA-N 0.000 description 1
- IRJWAYCXIYUHQE-WHFBIAKZSA-N Gly-Ser-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)CN IRJWAYCXIYUHQE-WHFBIAKZSA-N 0.000 description 1
- IALQAMYQJBZNSK-WHFBIAKZSA-N Gly-Ser-Asn Chemical compound [H]NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(O)=O IALQAMYQJBZNSK-WHFBIAKZSA-N 0.000 description 1
- WNGHUXFWEWTKAO-YUMQZZPRSA-N Gly-Ser-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)CN WNGHUXFWEWTKAO-YUMQZZPRSA-N 0.000 description 1
- RZEDHGORCKRINR-STQMWFEESA-N Gly-Trp-Cys Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)CN RZEDHGORCKRINR-STQMWFEESA-N 0.000 description 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
- JBCLFWXMTIKCCB-UHFFFAOYSA-N H-Gly-Phe-OH Natural products NCC(=O)NC(C(O)=O)CC1=CC=CC=C1 JBCLFWXMTIKCCB-UHFFFAOYSA-N 0.000 description 1
- HRGGKHFHRSFSDE-CIUDSAMLSA-N His-Asn-Ser Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CO)C(=O)O)N HRGGKHFHRSFSDE-CIUDSAMLSA-N 0.000 description 1
- LYSMQLXUCAKELQ-DCAQKATOSA-N His-Asp-Arg Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)N LYSMQLXUCAKELQ-DCAQKATOSA-N 0.000 description 1
- AIPUZFXMXAHZKY-QWRGUYRKSA-N His-Leu-Gly Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(O)=O AIPUZFXMXAHZKY-QWRGUYRKSA-N 0.000 description 1
- RNMNYMDTESKEAJ-KKUMJFAQSA-N His-Leu-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC1=CN=CN1 RNMNYMDTESKEAJ-KKUMJFAQSA-N 0.000 description 1
- IXQGOKWTQPCIQM-YJRXYDGGSA-N His-Thr-His Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)NC(=O)[C@H](CC2=CN=CN2)N)O IXQGOKWTQPCIQM-YJRXYDGGSA-N 0.000 description 1
- GGXUJBKENKVYNV-ULQDDVLXSA-N His-Val-Phe Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)NC(=O)[C@H](CC2=CN=CN2)N GGXUJBKENKVYNV-ULQDDVLXSA-N 0.000 description 1
- UAVQIQOOBXFKRC-BYULHYEWSA-N Ile-Asn-Gly Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(O)=O UAVQIQOOBXFKRC-BYULHYEWSA-N 0.000 description 1
- CTHAJJYOHOBUDY-GHCJXIJMSA-N Ile-Cys-Asp Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(=O)O)C(=O)O)N CTHAJJYOHOBUDY-GHCJXIJMSA-N 0.000 description 1
- ZDNORQNHCJUVOV-KBIXCLLPSA-N Ile-Gln-Ala Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(O)=O ZDNORQNHCJUVOV-KBIXCLLPSA-N 0.000 description 1
- HOLOYAZCIHDQNS-YVNDNENWSA-N Ile-Gln-Glu Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N HOLOYAZCIHDQNS-YVNDNENWSA-N 0.000 description 1
- KUHFPGIVBOCRMV-MNXVOIDGSA-N Ile-Gln-Leu Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CC(C)C)C(=O)O)N KUHFPGIVBOCRMV-MNXVOIDGSA-N 0.000 description 1
- MQFGXJNSUJTXDT-QSFUFRPTSA-N Ile-Gly-Ile Chemical compound N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H]([C@@H](C)CC)C(=O)O MQFGXJNSUJTXDT-QSFUFRPTSA-N 0.000 description 1
- YNMQUIVKEFRCPH-QSFUFRPTSA-N Ile-Ile-Gly Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)O)N YNMQUIVKEFRCPH-QSFUFRPTSA-N 0.000 description 1
- UAELWXJFLZBKQS-WHOFXGATSA-N Ile-Phe-Gly Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(O)=O UAELWXJFLZBKQS-WHOFXGATSA-N 0.000 description 1
- VEPIBPGLTLPBDW-URLPEUOOSA-N Ile-Phe-Thr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)O)C(=O)O)N VEPIBPGLTLPBDW-URLPEUOOSA-N 0.000 description 1
- IITVUURPOYGCTD-NAKRPEOUSA-N Ile-Pro-Ala Chemical compound CC[C@H](C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(O)=O IITVUURPOYGCTD-NAKRPEOUSA-N 0.000 description 1
- SVZFKLBRCYCIIY-CYDGBPFRSA-N Ile-Pro-Arg Chemical compound CC[C@H](C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(N)=N)C(O)=O SVZFKLBRCYCIIY-CYDGBPFRSA-N 0.000 description 1
- XHBYEMIUENPZLY-GMOBBJLQSA-N Ile-Pro-Asn Chemical compound CC[C@H](C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(N)=O)C(O)=O XHBYEMIUENPZLY-GMOBBJLQSA-N 0.000 description 1
- OWSWUWDMSNXTNE-GMOBBJLQSA-N Ile-Pro-Asp Chemical compound CC[C@H](C)[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)O)C(=O)O)N OWSWUWDMSNXTNE-GMOBBJLQSA-N 0.000 description 1
- NLZVTPYXYXMCIP-XUXIUFHCSA-N Ile-Pro-Lys Chemical compound CC[C@H](C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(O)=O NLZVTPYXYXMCIP-XUXIUFHCSA-N 0.000 description 1
- ZNOBVZFCHNHKHA-KBIXCLLPSA-N Ile-Ser-Glu Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N ZNOBVZFCHNHKHA-KBIXCLLPSA-N 0.000 description 1
- HXIDVIFHRYRXLZ-NAKRPEOUSA-N Ile-Ser-Val Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)O)N HXIDVIFHRYRXLZ-NAKRPEOUSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- SITWEMZOJNKJCH-UHFFFAOYSA-N L-alanine-L-arginine Natural products CC(N)C(=O)NC(C(O)=O)CCCNC(N)=N SITWEMZOJNKJCH-UHFFFAOYSA-N 0.000 description 1
- SENJXOPIZNYLHU-UHFFFAOYSA-N L-leucyl-L-arginine Natural products CC(C)CC(N)C(=O)NC(C(O)=O)CCCN=C(N)N SENJXOPIZNYLHU-UHFFFAOYSA-N 0.000 description 1
- 101150050624 LRR-RLK gene Proteins 0.000 description 1
- CZCSUZMIRKFFFA-CIUDSAMLSA-N Leu-Ala-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(O)=O CZCSUZMIRKFFFA-CIUDSAMLSA-N 0.000 description 1
- PBCHMHROGNUXMK-DLOVCJGASA-N Leu-Ala-His Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CC1=CN=CN1 PBCHMHROGNUXMK-DLOVCJGASA-N 0.000 description 1
- XBBKIIGCUMBKCO-JXUBOQSCSA-N Leu-Ala-Thr Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O XBBKIIGCUMBKCO-JXUBOQSCSA-N 0.000 description 1
- JKGHDYGZRDWHGA-SRVKXCTJSA-N Leu-Asn-Leu Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(O)=O JKGHDYGZRDWHGA-SRVKXCTJSA-N 0.000 description 1
- OGCQGUIWMSBHRZ-CIUDSAMLSA-N Leu-Asn-Ser Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(O)=O OGCQGUIWMSBHRZ-CIUDSAMLSA-N 0.000 description 1
- YKNBJXOJTURHCU-DCAQKATOSA-N Leu-Asp-Arg Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(O)=O)CCCN=C(N)N YKNBJXOJTURHCU-DCAQKATOSA-N 0.000 description 1
- MYGQXVYRZMKRDB-SRVKXCTJSA-N Leu-Asp-Lys Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(O)=O)CCCCN MYGQXVYRZMKRDB-SRVKXCTJSA-N 0.000 description 1
- KWURTLAFFDOTEQ-GUBZILKMSA-N Leu-Cys-Glu Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N KWURTLAFFDOTEQ-GUBZILKMSA-N 0.000 description 1
- PPBKJAQJAUHZKX-SRVKXCTJSA-N Leu-Cys-Leu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@H](C(O)=O)CC(C)C PPBKJAQJAUHZKX-SRVKXCTJSA-N 0.000 description 1
- VQPPIMUZCZCOIL-GUBZILKMSA-N Leu-Gln-Ala Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(O)=O VQPPIMUZCZCOIL-GUBZILKMSA-N 0.000 description 1
- DPWGZWUMUUJQDT-IUCAKERBSA-N Leu-Gln-Gly Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(O)=O DPWGZWUMUUJQDT-IUCAKERBSA-N 0.000 description 1
- FMEICTQWUKNAGC-YUMQZZPRSA-N Leu-Gly-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(O)=O FMEICTQWUKNAGC-YUMQZZPRSA-N 0.000 description 1
- LAPSXOAUPNOINL-YUMQZZPRSA-N Leu-Gly-Asp Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC(O)=O LAPSXOAUPNOINL-YUMQZZPRSA-N 0.000 description 1
- YWYQSLOTVIRCFE-SRVKXCTJSA-N Leu-His-Asp Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(O)=O)C(O)=O YWYQSLOTVIRCFE-SRVKXCTJSA-N 0.000 description 1
- HNDWYLYAYNBWMP-AJNGGQMLSA-N Leu-Ile-Lys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)O)NC(=O)[C@H](CC(C)C)N HNDWYLYAYNBWMP-AJNGGQMLSA-N 0.000 description 1
- LIINDKYIGYTDLG-PPCPHDFISA-N Leu-Ile-Thr Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(O)=O LIINDKYIGYTDLG-PPCPHDFISA-N 0.000 description 1
- DSFYPIUSAMSERP-IHRRRGAJSA-N Leu-Leu-Arg Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(O)=O)CCCN=C(N)N DSFYPIUSAMSERP-IHRRRGAJSA-N 0.000 description 1
- IAJFFZORSWOZPQ-SRVKXCTJSA-N Leu-Leu-Asn Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O IAJFFZORSWOZPQ-SRVKXCTJSA-N 0.000 description 1
- FOBUGKUBUJOWAD-IHPCNDPISA-N Leu-Leu-Trp Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC(C)C)C(O)=O)=CNC2=C1 FOBUGKUBUJOWAD-IHPCNDPISA-N 0.000 description 1
- GNRPTBRHRRZCMA-RWMBFGLXSA-N Leu-Met-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCSC)C(=O)N1CCC[C@@H]1C(=O)O)N GNRPTBRHRRZCMA-RWMBFGLXSA-N 0.000 description 1
- YUTNOGOMBNYPFH-XUXIUFHCSA-N Leu-Pro-Ile Chemical compound [H]N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)CC)C(O)=O YUTNOGOMBNYPFH-XUXIUFHCSA-N 0.000 description 1
- CHJKEDSZNSONPS-DCAQKATOSA-N Leu-Pro-Ser Chemical compound [H]N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O CHJKEDSZNSONPS-DCAQKATOSA-N 0.000 description 1
- JIHDFWWRYHSAQB-GUBZILKMSA-N Leu-Ser-Glu Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCC(O)=O JIHDFWWRYHSAQB-GUBZILKMSA-N 0.000 description 1
- ADJWHHZETYAAAX-SRVKXCTJSA-N Leu-Ser-His Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N ADJWHHZETYAAAX-SRVKXCTJSA-N 0.000 description 1
- SBANPBVRHYIMRR-GARJFASQSA-N Leu-Ser-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CO)C(=O)N1CCC[C@@H]1C(=O)O)N SBANPBVRHYIMRR-GARJFASQSA-N 0.000 description 1
- SBANPBVRHYIMRR-UHFFFAOYSA-N Leu-Ser-Pro Natural products CC(C)CC(N)C(=O)NC(CO)C(=O)N1CCCC1C(O)=O SBANPBVRHYIMRR-UHFFFAOYSA-N 0.000 description 1
- ZJZNLRVCZWUONM-JXUBOQSCSA-N Leu-Thr-Ala Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(O)=O ZJZNLRVCZWUONM-JXUBOQSCSA-N 0.000 description 1
- LCNASHSOFMRYFO-WDCWCFNPSA-N Leu-Thr-Gln Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CCC(N)=O LCNASHSOFMRYFO-WDCWCFNPSA-N 0.000 description 1
- VDIARPPNADFEAV-WEDXCCLWSA-N Leu-Thr-Gly Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(O)=O VDIARPPNADFEAV-WEDXCCLWSA-N 0.000 description 1
- URHJPNHRQMQGOZ-RHYQMDGZSA-N Leu-Thr-Met Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCSC)C(O)=O URHJPNHRQMQGOZ-RHYQMDGZSA-N 0.000 description 1
- HOMFINRJHIIZNJ-HOCLYGCPSA-N Leu-Trp-Gly Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)NCC(O)=O HOMFINRJHIIZNJ-HOCLYGCPSA-N 0.000 description 1
- XZNJZXJZBMBGGS-NHCYSSNCSA-N Leu-Val-Asn Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O XZNJZXJZBMBGGS-NHCYSSNCSA-N 0.000 description 1
- FDBTVENULFNTAL-XQQFMLRXSA-N Leu-Val-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N1CCC[C@@H]1C(=O)O)N FDBTVENULFNTAL-XQQFMLRXSA-N 0.000 description 1
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
- 235000002262 Lycopersicon Nutrition 0.000 description 1
- JEVVKJMRZMXFBT-XWDZUXABSA-N Lycophyll Natural products OC/C(=C/CC/C(=C\C=C\C(=C/C=C/C(=C\C=C\C=C(/C=C/C=C(\C=C\C=C(/CC/C=C(/CO)\C)\C)/C)\C)/C)\C)/C)/C JEVVKJMRZMXFBT-XWDZUXABSA-N 0.000 description 1
- KNKHAVVBVXKOGX-JXUBOQSCSA-N Lys-Ala-Thr Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O KNKHAVVBVXKOGX-JXUBOQSCSA-N 0.000 description 1
- YNNPKXBBRZVIRX-IHRRRGAJSA-N Lys-Arg-Leu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(C)C)C(O)=O YNNPKXBBRZVIRX-IHRRRGAJSA-N 0.000 description 1
- ABHIXYDMILIUKV-CIUDSAMLSA-N Lys-Asn-Asn Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O ABHIXYDMILIUKV-CIUDSAMLSA-N 0.000 description 1
- YKIRNDPUWONXQN-GUBZILKMSA-N Lys-Asn-Gln Chemical compound C(CCN)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N YKIRNDPUWONXQN-GUBZILKMSA-N 0.000 description 1
- QUCDKEKDPYISNX-HJGDQZAQSA-N Lys-Asn-Thr Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O QUCDKEKDPYISNX-HJGDQZAQSA-N 0.000 description 1
- SVJRVFPSHPGWFF-DCAQKATOSA-N Lys-Cys-Arg Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O SVJRVFPSHPGWFF-DCAQKATOSA-N 0.000 description 1
- NDORZBUHCOJQDO-GVXVVHGQSA-N Lys-Gln-Val Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C(C)C)C(O)=O NDORZBUHCOJQDO-GVXVVHGQSA-N 0.000 description 1
- OVAOHZIOUBEQCJ-IHRRRGAJSA-N Lys-Leu-Arg Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O OVAOHZIOUBEQCJ-IHRRRGAJSA-N 0.000 description 1
- SKRGVGLIRUGANF-AVGNSLFASA-N Lys-Leu-Glu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(O)=O)C(O)=O SKRGVGLIRUGANF-AVGNSLFASA-N 0.000 description 1
- XIZQPFCRXLUNMK-BZSNNMDCSA-N Lys-Leu-Phe Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)NC(=O)[C@H](CCCCN)N XIZQPFCRXLUNMK-BZSNNMDCSA-N 0.000 description 1
- WRODMZBHNNPRLN-SRVKXCTJSA-N Lys-Leu-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O WRODMZBHNNPRLN-SRVKXCTJSA-N 0.000 description 1
- HVAUKHLDSDDROB-KKUMJFAQSA-N Lys-Lys-Leu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(O)=O HVAUKHLDSDDROB-KKUMJFAQSA-N 0.000 description 1
- KJIXWRWPOCKYLD-IHRRRGAJSA-N Lys-Lys-Met Chemical compound CSCC[C@@H](C(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)N KJIXWRWPOCKYLD-IHRRRGAJSA-N 0.000 description 1
- LUTDBHBIHHREDC-IHRRRGAJSA-N Lys-Pro-Lys Chemical compound NCCCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(O)=O LUTDBHBIHHREDC-IHRRRGAJSA-N 0.000 description 1
- SQXZLVXQXWILKW-KKUMJFAQSA-N Lys-Ser-Phe Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O SQXZLVXQXWILKW-KKUMJFAQSA-N 0.000 description 1
- DIBZLYZXTSVGLN-CIUDSAMLSA-N Lys-Ser-Ser Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O DIBZLYZXTSVGLN-CIUDSAMLSA-N 0.000 description 1
- MIMXMVDLMDMOJD-BZSNNMDCSA-N Lys-Tyr-Leu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(C)C)C(O)=O MIMXMVDLMDMOJD-BZSNNMDCSA-N 0.000 description 1
- VKCPHIOZDWUFSW-ONGXEEELSA-N Lys-Val-Gly Chemical compound OC(=O)CNC(=O)[C@H](C(C)C)NC(=O)[C@@H](N)CCCCN VKCPHIOZDWUFSW-ONGXEEELSA-N 0.000 description 1
- RPWQJSBMXJSCPD-XUXIUFHCSA-N Lys-Val-Ile Chemical compound CC[C@H](C)[C@H](NC(=O)[C@@H](NC(=O)[C@@H](N)CCCCN)C(C)C)C(O)=O RPWQJSBMXJSCPD-XUXIUFHCSA-N 0.000 description 1
- JQEBITVYKUCBMC-SRVKXCTJSA-N Met-Arg-Arg Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O JQEBITVYKUCBMC-SRVKXCTJSA-N 0.000 description 1
- DGNZGCQSVGGYJS-BQBZGAKWSA-N Met-Gly-Asp Chemical compound CSCC[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC(O)=O DGNZGCQSVGGYJS-BQBZGAKWSA-N 0.000 description 1
- QZPXMHVKPHJNTR-DCAQKATOSA-N Met-Leu-Asn Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O QZPXMHVKPHJNTR-DCAQKATOSA-N 0.000 description 1
- XDGFFEZAZHRZFR-RHYQMDGZSA-N Met-Leu-Thr Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O XDGFFEZAZHRZFR-RHYQMDGZSA-N 0.000 description 1
- JQHYVIKEFYETEW-IHRRRGAJSA-N Met-Phe-Ser Chemical compound CSCC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CO)C(O)=O)CC1=CC=CC=C1 JQHYVIKEFYETEW-IHRRRGAJSA-N 0.000 description 1
- RMLLCGYYVZKKRT-CIUDSAMLSA-N Met-Ser-Glu Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCC(O)=O RMLLCGYYVZKKRT-CIUDSAMLSA-N 0.000 description 1
- HLZORBMOISUNIV-DCAQKATOSA-N Met-Ser-Leu Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CC(C)C HLZORBMOISUNIV-DCAQKATOSA-N 0.000 description 1
- FXBKQTOGURNXSL-HJGDQZAQSA-N Met-Thr-Glu Chemical compound CSCC[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CCC(O)=O FXBKQTOGURNXSL-HJGDQZAQSA-N 0.000 description 1
- XTSBLBXAUIBMLW-KKUMJFAQSA-N Met-Tyr-Glu Chemical compound CSCC[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)N[C@@H](CCC(=O)O)C(=O)O)N XTSBLBXAUIBMLW-KKUMJFAQSA-N 0.000 description 1
- 101100457341 Mus musculus Mapk13 gene Proteins 0.000 description 1
- BQVUABVGYYSDCJ-UHFFFAOYSA-N Nalpha-L-Leucyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)C(N)CC(C)C)C(O)=O)=CNC2=C1 BQVUABVGYYSDCJ-UHFFFAOYSA-N 0.000 description 1
- 206010028851 Necrosis Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 101100203024 Oryza sativa subsp. japonica SERK3 gene Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- HTTYNOXBBOWZTB-SRVKXCTJSA-N Phe-Asn-Asn Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC(=O)N)C(=O)O)N HTTYNOXBBOWZTB-SRVKXCTJSA-N 0.000 description 1
- HGNGAMWHGGANAU-WHOFXGATSA-N Phe-Gly-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)CC1=CC=CC=C1 HGNGAMWHGGANAU-WHOFXGATSA-N 0.000 description 1
- BIYWZVCPZIFGPY-QWRGUYRKSA-N Phe-Gly-Ser Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)NCC(=O)N[C@@H](CO)C(O)=O BIYWZVCPZIFGPY-QWRGUYRKSA-N 0.000 description 1
- BYAIIACBWBOJCU-URLPEUOOSA-N Phe-Ile-Thr Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(O)=O BYAIIACBWBOJCU-URLPEUOOSA-N 0.000 description 1
- JQLQUPIYYJXZLJ-ZEWNOJEFSA-N Phe-Ile-Tyr Chemical compound C([C@H](N)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)C1=CC=CC=C1 JQLQUPIYYJXZLJ-ZEWNOJEFSA-N 0.000 description 1
- KBVJZCVLQWCJQN-KKUMJFAQSA-N Phe-Leu-Asn Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O KBVJZCVLQWCJQN-KKUMJFAQSA-N 0.000 description 1
- SMFGCTXUBWEPKM-KBPBESRZSA-N Phe-Leu-Gly Chemical compound OC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC1=CC=CC=C1 SMFGCTXUBWEPKM-KBPBESRZSA-N 0.000 description 1
- KZRQONDKKJCAOL-DKIMLUQUSA-N Phe-Leu-Ile Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O KZRQONDKKJCAOL-DKIMLUQUSA-N 0.000 description 1
- YTILBRIUASDGBL-BZSNNMDCSA-N Phe-Leu-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC1=CC=CC=C1 YTILBRIUASDGBL-BZSNNMDCSA-N 0.000 description 1
- BSHMIVKDJQGLNT-ACRUOGEOSA-N Phe-Lys-Tyr Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(O)=O)C1=CC=CC=C1 BSHMIVKDJQGLNT-ACRUOGEOSA-N 0.000 description 1
- FENSZYFJQOFSQR-FIRPJDEBSA-N Phe-Phe-Ile Chemical compound C([C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(O)=O)NC(=O)[C@@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FENSZYFJQOFSQR-FIRPJDEBSA-N 0.000 description 1
- AFNJAQVMTIQTCB-DLOVCJGASA-N Phe-Ser-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC1=CC=CC=C1 AFNJAQVMTIQTCB-DLOVCJGASA-N 0.000 description 1
- YMIZSYUAZJSOFL-SRVKXCTJSA-N Phe-Ser-Asn Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(O)=O YMIZSYUAZJSOFL-SRVKXCTJSA-N 0.000 description 1
- BONHGTUEEPIMPM-AVGNSLFASA-N Phe-Ser-Glu Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(O)=O BONHGTUEEPIMPM-AVGNSLFASA-N 0.000 description 1
- BPCLGWHVPVTTFM-QWRGUYRKSA-N Phe-Ser-Gly Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(=O)NCC(O)=O BPCLGWHVPVTTFM-QWRGUYRKSA-N 0.000 description 1
- ABEFOXGAIIJDCL-SFJXLCSZSA-N Phe-Thr-Trp Chemical compound C([C@H](N)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(O)=O)C1=CC=CC=C1 ABEFOXGAIIJDCL-SFJXLCSZSA-N 0.000 description 1
- KQCCDMFIALWGTL-GUBZILKMSA-N Pro-Asn-Met Chemical compound CSCC[C@@H](C(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H]1CCCN1 KQCCDMFIALWGTL-GUBZILKMSA-N 0.000 description 1
- HJSCRFZVGXAGNG-SRVKXCTJSA-N Pro-Gln-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H]1CCCN1 HJSCRFZVGXAGNG-SRVKXCTJSA-N 0.000 description 1
- WVOXLKUUVCCCSU-ZPFDUUQYSA-N Pro-Glu-Ile Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O WVOXLKUUVCCCSU-ZPFDUUQYSA-N 0.000 description 1
- QGOZJLYCGRYYRW-KKUMJFAQSA-N Pro-Glu-Tyr Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O QGOZJLYCGRYYRW-KKUMJFAQSA-N 0.000 description 1
- ULIWFCCJIOEHMU-BQBZGAKWSA-N Pro-Gly-Asp Chemical compound OC(=O)C[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H]1CCCN1 ULIWFCCJIOEHMU-BQBZGAKWSA-N 0.000 description 1
- FEPSEIDIPBMIOS-QXEWZRGKSA-N Pro-Gly-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H]1CCCN1 FEPSEIDIPBMIOS-QXEWZRGKSA-N 0.000 description 1
- DXTOOBDIIAJZBJ-BQBZGAKWSA-N Pro-Gly-Ser Chemical compound [H]N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CO)C(O)=O DXTOOBDIIAJZBJ-BQBZGAKWSA-N 0.000 description 1
- QEWBZBLXDKIQPS-STQMWFEESA-N Pro-Gly-Tyr Chemical compound [H]N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O QEWBZBLXDKIQPS-STQMWFEESA-N 0.000 description 1
- YTWNSIDWAFSEEI-RWMBFGLXSA-N Pro-His-Pro Chemical compound C1C[C@H](NC1)C(=O)N[C@@H](CC2=CN=CN2)C(=O)N3CCC[C@@H]3C(=O)O YTWNSIDWAFSEEI-RWMBFGLXSA-N 0.000 description 1
- FKVNLUZHSFCNGY-RVMXOQNASA-N Pro-Ile-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@@H]2CCCN2 FKVNLUZHSFCNGY-RVMXOQNASA-N 0.000 description 1
- MCWHYUWXVNRXFV-RWMBFGLXSA-N Pro-Leu-Pro Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@@H]2CCCN2 MCWHYUWXVNRXFV-RWMBFGLXSA-N 0.000 description 1
- GFHXZNVJIKMAGO-IHRRRGAJSA-N Pro-Phe-Ser Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CO)C(O)=O GFHXZNVJIKMAGO-IHRRRGAJSA-N 0.000 description 1
- QKDIHFHGHBYTKB-IHRRRGAJSA-N Pro-Ser-Phe Chemical compound N([C@@H](CO)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C(=O)[C@@H]1CCCN1 QKDIHFHGHBYTKB-IHRRRGAJSA-N 0.000 description 1
- MKGIILKDUGDRRO-FXQIFTODSA-N Pro-Ser-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H]1CCCN1 MKGIILKDUGDRRO-FXQIFTODSA-N 0.000 description 1
- XSXABUHLKPUVLX-JYJNAYRXSA-N Pro-Ser-Trp Chemical compound C1C[C@H](NC1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC2=CNC3=CC=CC=C32)C(=O)O XSXABUHLKPUVLX-JYJNAYRXSA-N 0.000 description 1
- FIODMZKLZFLYQP-GUBZILKMSA-N Pro-Val-Ser Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CO)C(O)=O FIODMZKLZFLYQP-GUBZILKMSA-N 0.000 description 1
- 101800000795 Proadrenomedullin N-20 terminal peptide Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000589626 Pseudomonas syringae pv. tomato Species 0.000 description 1
- 108010079005 RDV peptide Proteins 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 238000010802 RNA extraction kit Methods 0.000 description 1
- 101100209986 Rattus norvegicus Slc18a1 gene Proteins 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- 101150074305 SERK4 gene Proteins 0.000 description 1
- QVOGDCQNGLBNCR-FXQIFTODSA-N Ser-Arg-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CO)C(O)=O QVOGDCQNGLBNCR-FXQIFTODSA-N 0.000 description 1
- OBXVZEAMXFSGPU-FXQIFTODSA-N Ser-Asn-Arg Chemical compound C(C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@H](CO)N)CN=C(N)N OBXVZEAMXFSGPU-FXQIFTODSA-N 0.000 description 1
- OOKCGAYXSNJBGQ-ZLUOBGJFSA-N Ser-Asn-Asn Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O OOKCGAYXSNJBGQ-ZLUOBGJFSA-N 0.000 description 1
- FIDMVVBUOCMMJG-CIUDSAMLSA-N Ser-Asn-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](N)CO FIDMVVBUOCMMJG-CIUDSAMLSA-N 0.000 description 1
- COAHUSQNSVFYBW-FXQIFTODSA-N Ser-Asn-Met Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCSC)C(O)=O COAHUSQNSVFYBW-FXQIFTODSA-N 0.000 description 1
- OHKLFYXEOGGGCK-ZLUOBGJFSA-N Ser-Asp-Asn Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O OHKLFYXEOGGGCK-ZLUOBGJFSA-N 0.000 description 1
- BGOWRLSWJCVYAQ-CIUDSAMLSA-N Ser-Asp-Leu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O BGOWRLSWJCVYAQ-CIUDSAMLSA-N 0.000 description 1
- MPPHJZYXDVDGOF-BWBBJGPYSA-N Ser-Cys-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H](CS)NC(=O)[C@@H](N)CO MPPHJZYXDVDGOF-BWBBJGPYSA-N 0.000 description 1
- SQBLRDDJTUJDMV-ACZMJKKPSA-N Ser-Glu-Asn Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O SQBLRDDJTUJDMV-ACZMJKKPSA-N 0.000 description 1
- VQBCMLMPEWPUTB-ACZMJKKPSA-N Ser-Glu-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CO)C(O)=O VQBCMLMPEWPUTB-ACZMJKKPSA-N 0.000 description 1
- AEGUWTFAQQWVLC-BQBZGAKWSA-N Ser-Gly-Arg Chemical compound [H]N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(O)=O AEGUWTFAQQWVLC-BQBZGAKWSA-N 0.000 description 1
- MIJWOJAXARLEHA-WDSKDSINSA-N Ser-Gly-Glu Chemical compound OC[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCC(O)=O MIJWOJAXARLEHA-WDSKDSINSA-N 0.000 description 1
- GZFAWAQTEYDKII-YUMQZZPRSA-N Ser-Gly-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)CO GZFAWAQTEYDKII-YUMQZZPRSA-N 0.000 description 1
- UIGMAMGZOJVTDN-WHFBIAKZSA-N Ser-Gly-Ser Chemical compound OC[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O UIGMAMGZOJVTDN-WHFBIAKZSA-N 0.000 description 1
- CXBFHZLODKPIJY-AAEUAGOBSA-N Ser-Gly-Trp Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)O)NC(=O)CNC(=O)[C@H](CO)N CXBFHZLODKPIJY-AAEUAGOBSA-N 0.000 description 1
- RJHJPZQOMKCSTP-CIUDSAMLSA-N Ser-His-Asn Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(N)=O)C(O)=O RJHJPZQOMKCSTP-CIUDSAMLSA-N 0.000 description 1
- LOKXAXAESFYFAX-CIUDSAMLSA-N Ser-His-Cys Chemical compound OC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CS)C(O)=O)CC1=CN=CN1 LOKXAXAESFYFAX-CIUDSAMLSA-N 0.000 description 1
- IFPBAGJBHSNYPR-ZKWXMUAHSA-N Ser-Ile-Gly Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(O)=O IFPBAGJBHSNYPR-ZKWXMUAHSA-N 0.000 description 1
- VMLONWHIORGALA-SRVKXCTJSA-N Ser-Leu-Leu Chemical compound CC(C)C[C@@H](C([O-])=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]([NH3+])CO VMLONWHIORGALA-SRVKXCTJSA-N 0.000 description 1
- WGDYNRCOQRERLZ-KKUMJFAQSA-N Ser-Lys-Phe Chemical compound C1=CC=C(C=C1)C[C@@H](C(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)N WGDYNRCOQRERLZ-KKUMJFAQSA-N 0.000 description 1
- QSHKTZVJGDVFEW-GUBZILKMSA-N Ser-Met-Met Chemical compound CSCC[C@@H](C(=O)N[C@@H](CCSC)C(=O)O)NC(=O)[C@H](CO)N QSHKTZVJGDVFEW-GUBZILKMSA-N 0.000 description 1
- TVPQRPNBYCRRLL-IHRRRGAJSA-N Ser-Phe-Met Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCSC)C(O)=O TVPQRPNBYCRRLL-IHRRRGAJSA-N 0.000 description 1
- AZWNCEBQZXELEZ-FXQIFTODSA-N Ser-Pro-Ser Chemical compound OC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O AZWNCEBQZXELEZ-FXQIFTODSA-N 0.000 description 1
- KQNDIKOYWZTZIX-FXQIFTODSA-N Ser-Ser-Arg Chemical compound OC[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CCCNC(N)=N KQNDIKOYWZTZIX-FXQIFTODSA-N 0.000 description 1
- SRSPTFBENMJHMR-WHFBIAKZSA-N Ser-Ser-Gly Chemical compound OC[C@H](N)C(=O)N[C@@H](CO)C(=O)NCC(O)=O SRSPTFBENMJHMR-WHFBIAKZSA-N 0.000 description 1
- UYLKOSODXYSWMQ-XGEHTFHBSA-N Ser-Thr-Met Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCSC)C(=O)O)NC(=O)[C@H](CO)N)O UYLKOSODXYSWMQ-XGEHTFHBSA-N 0.000 description 1
- FHXGMDRKJHKLKW-QWRGUYRKSA-N Ser-Tyr-Gly Chemical compound OC[C@H](N)C(=O)N[C@H](C(=O)NCC(O)=O)CC1=CC=C(O)C=C1 FHXGMDRKJHKLKW-QWRGUYRKSA-N 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 235000002634 Solanum Nutrition 0.000 description 1
- 241000207763 Solanum Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 101100108497 Sus scrofa AKR1A1 gene Proteins 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- DWYAUVCQDTZIJI-VZFHVOOUSA-N Thr-Ala-Ser Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O DWYAUVCQDTZIJI-VZFHVOOUSA-N 0.000 description 1
- SWIKDOUVROTZCW-GCJQMDKQSA-N Thr-Asn-Ala Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](C)C(=O)O)N)O SWIKDOUVROTZCW-GCJQMDKQSA-N 0.000 description 1
- YLXAMFZYJTZXFH-OLHMAJIHSA-N Thr-Asn-Asp Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CC(=O)O)C(=O)O)N)O YLXAMFZYJTZXFH-OLHMAJIHSA-N 0.000 description 1
- GNHRVXYZKWSJTF-HJGDQZAQSA-N Thr-Asp-Lys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCCCN)C(=O)O)N)O GNHRVXYZKWSJTF-HJGDQZAQSA-N 0.000 description 1
- KRPKYGOFYUNIGM-XVSYOHENSA-N Thr-Asp-Phe Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)N)O KRPKYGOFYUNIGM-XVSYOHENSA-N 0.000 description 1
- SHOMROOOQBDGRL-JHEQGTHGSA-N Thr-Glu-Gly Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(O)=O SHOMROOOQBDGRL-JHEQGTHGSA-N 0.000 description 1
- XFTYVCHLARBHBQ-FOHZUACHSA-N Thr-Gly-Asn Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(O)=O XFTYVCHLARBHBQ-FOHZUACHSA-N 0.000 description 1
- FKIGTIXHSRNKJU-IXOXFDKPSA-N Thr-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)[C@H](O)C)CC1=CN=CN1 FKIGTIXHSRNKJU-IXOXFDKPSA-N 0.000 description 1
- KRGDDWVBBDLPSJ-CUJWVEQBSA-N Thr-His-Ser Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(O)=O KRGDDWVBBDLPSJ-CUJWVEQBSA-N 0.000 description 1
- URPSJRMWHQTARR-MBLNEYKQSA-N Thr-Ile-Gly Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(O)=O URPSJRMWHQTARR-MBLNEYKQSA-N 0.000 description 1
- JLNMFGCJODTXDH-WEDXCCLWSA-N Thr-Lys-Gly Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCCN)C(=O)NCC(O)=O JLNMFGCJODTXDH-WEDXCCLWSA-N 0.000 description 1
- WYLAVUAWOUVUCA-XVSYOHENSA-N Thr-Phe-Asp Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(O)=O)C(O)=O WYLAVUAWOUVUCA-XVSYOHENSA-N 0.000 description 1
- WKGAAMOJPMBBMC-IXOXFDKPSA-N Thr-Ser-Phe Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O WKGAAMOJPMBBMC-IXOXFDKPSA-N 0.000 description 1
- VUXIQSUQQYNLJP-XAVMHZPKSA-N Thr-Ser-Pro Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CO)C(=O)N1CCC[C@@H]1C(=O)O)N)O VUXIQSUQQYNLJP-XAVMHZPKSA-N 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- YLGQHMHKAASRGJ-WDSOQIARSA-N Trp-Leu-Met Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCSC)C(=O)O)NC(=O)[C@H](CC1=CNC2=CC=CC=C21)N YLGQHMHKAASRGJ-WDSOQIARSA-N 0.000 description 1
- AKXBNSZMYAOGLS-STQMWFEESA-N Tyr-Arg-Gly Chemical compound NC(N)=NCCC[C@@H](C(=O)NCC(O)=O)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 AKXBNSZMYAOGLS-STQMWFEESA-N 0.000 description 1
- MBFJIHUHHCJBSN-AVGNSLFASA-N Tyr-Asn-Gln Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O MBFJIHUHHCJBSN-AVGNSLFASA-N 0.000 description 1
- NSTPFWRAIDTNGH-BZSNNMDCSA-N Tyr-Asn-Tyr Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O NSTPFWRAIDTNGH-BZSNNMDCSA-N 0.000 description 1
- AKLNEFNQWLHIGY-QWRGUYRKSA-N Tyr-Gly-Asp Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)O)N)O AKLNEFNQWLHIGY-QWRGUYRKSA-N 0.000 description 1
- YMUQBRQQCPQEQN-CXTHYWKRSA-N Tyr-Ile-Tyr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CC1=CC=C(C=C1)O)C(=O)O)NC(=O)[C@H](CC2=CC=C(C=C2)O)N YMUQBRQQCPQEQN-CXTHYWKRSA-N 0.000 description 1
- XDGPTBVOSHKDFT-KKUMJFAQSA-N Tyr-Met-Glu Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(O)=O)C(O)=O XDGPTBVOSHKDFT-KKUMJFAQSA-N 0.000 description 1
- ZZDYJFVIKVSUFA-WLTAIBSBSA-N Tyr-Thr-Gly Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(O)=O ZZDYJFVIKVSUFA-WLTAIBSBSA-N 0.000 description 1
- RGJZPXFZIUUQDN-BPNCWPANSA-N Tyr-Val-Ala Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(O)=O RGJZPXFZIUUQDN-BPNCWPANSA-N 0.000 description 1
- DDRBQONWVBDQOY-GUBZILKMSA-N Val-Ala-Arg Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O DDRBQONWVBDQOY-GUBZILKMSA-N 0.000 description 1
- ASQFIHTXXMFENG-XPUUQOCRSA-N Val-Ala-Gly Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](C)C(=O)NCC(O)=O ASQFIHTXXMFENG-XPUUQOCRSA-N 0.000 description 1
- ZLFHAAGHGQBQQN-GUBZILKMSA-N Val-Ala-Pro Natural products CC(C)[C@H](N)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(O)=O ZLFHAAGHGQBQQN-GUBZILKMSA-N 0.000 description 1
- IVXJODPZRWHCCR-JYJNAYRXSA-N Val-Arg-Phe Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)O)N IVXJODPZRWHCCR-JYJNAYRXSA-N 0.000 description 1
- FPCIBLUVDNXPJO-XPUUQOCRSA-N Val-Cys-Gly Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CS)C(=O)NCC(O)=O FPCIBLUVDNXPJO-XPUUQOCRSA-N 0.000 description 1
- VVZDBPBZHLQPPB-XVKPBYJWSA-N Val-Glu-Gly Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(O)=O VVZDBPBZHLQPPB-XVKPBYJWSA-N 0.000 description 1
- VCAWFLIWYNMHQP-UKJIMTQDSA-N Val-Glu-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](C(C)C)N VCAWFLIWYNMHQP-UKJIMTQDSA-N 0.000 description 1
- ROLGIBMFNMZANA-GVXVVHGQSA-N Val-Glu-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](C(C)C)N ROLGIBMFNMZANA-GVXVVHGQSA-N 0.000 description 1
- OQWNEUXPKHIEJO-NRPADANISA-N Val-Glu-Ser Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CO)C(=O)O)N OQWNEUXPKHIEJO-NRPADANISA-N 0.000 description 1
- RKIGNDAHUOOIMJ-BQFCYCMXSA-N Val-Glu-Trp Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)C(C)C)C(O)=O)=CNC2=C1 RKIGNDAHUOOIMJ-BQFCYCMXSA-N 0.000 description 1
- DJEVQCWNMQOABE-RCOVLWMOSA-N Val-Gly-Asp Chemical compound CC(C)[C@@H](C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)O)N DJEVQCWNMQOABE-RCOVLWMOSA-N 0.000 description 1
- HQYVQDRYODWONX-DCAQKATOSA-N Val-His-Ser Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)N[C@@H](CO)C(=O)O)N HQYVQDRYODWONX-DCAQKATOSA-N 0.000 description 1
- FEXILLGKGGTLRI-NHCYSSNCSA-N Val-Leu-Asn Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)[C@H](C(C)C)N FEXILLGKGGTLRI-NHCYSSNCSA-N 0.000 description 1
- AGXGCFSECFQMKB-NHCYSSNCSA-N Val-Leu-Asp Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)O)NC(=O)[C@H](C(C)C)N AGXGCFSECFQMKB-NHCYSSNCSA-N 0.000 description 1
- XTDDIVQWDXMRJL-IHRRRGAJSA-N Val-Leu-His Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)NC(=O)[C@H](C(C)C)N XTDDIVQWDXMRJL-IHRRRGAJSA-N 0.000 description 1
- ZHQWPWQNVRCXAX-XQQFMLRXSA-N Val-Leu-Pro Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](C(C)C)N ZHQWPWQNVRCXAX-XQQFMLRXSA-N 0.000 description 1
- RWOGENDAOGMHLX-DCAQKATOSA-N Val-Lys-Ala Chemical compound C[C@@H](C(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C(C)C)N RWOGENDAOGMHLX-DCAQKATOSA-N 0.000 description 1
- YMTOEGGOCHVGEH-IHRRRGAJSA-N Val-Lys-Lys Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(O)=O YMTOEGGOCHVGEH-IHRRRGAJSA-N 0.000 description 1
- JVGHIFMSFBZDHH-WPRPVWTQSA-N Val-Met-Gly Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCSC)C(=O)NCC(=O)O)N JVGHIFMSFBZDHH-WPRPVWTQSA-N 0.000 description 1
- HJSLDXZAZGFPDK-ULQDDVLXSA-N Val-Phe-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=CC=C1)NC(=O)[C@H](C(C)C)N HJSLDXZAZGFPDK-ULQDDVLXSA-N 0.000 description 1
- YTNGABPUXFEOGU-SRVKXCTJSA-N Val-Pro-Arg Chemical compound CC(C)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C(N)N)C(O)=O YTNGABPUXFEOGU-SRVKXCTJSA-N 0.000 description 1
- RYHUIHUOYRNNIE-NRPADANISA-N Val-Ser-Gln Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N RYHUIHUOYRNNIE-NRPADANISA-N 0.000 description 1
- ZHWZDZFWBXWPDW-GUBZILKMSA-N Val-Val-Cys Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(O)=O ZHWZDZFWBXWPDW-GUBZILKMSA-N 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 239000011543 agarose gel Substances 0.000 description 1
- 108010041407 alanylaspartic acid Proteins 0.000 description 1
- 108010005233 alanylglutamic acid Proteins 0.000 description 1
- 101150063729 alr1 gene Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 108010013835 arginine glutamate Proteins 0.000 description 1
- 108010018691 arginyl-threonyl-arginine Proteins 0.000 description 1
- 108010060035 arginylproline Proteins 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000007783 downstream signaling Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 235000019441 ethanol Nutrition 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- XBGGUPMXALFZOT-UHFFFAOYSA-N glycyl-L-tyrosine hemihydrate Natural products NCC(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 XBGGUPMXALFZOT-UHFFFAOYSA-N 0.000 description 1
- 108010027668 glycyl-alanyl-valine Proteins 0.000 description 1
- 108010019832 glycyl-asparaginyl-glycine Proteins 0.000 description 1
- 108010077435 glycyl-phenylalanyl-glycine Proteins 0.000 description 1
- 108010082286 glycyl-seryl-alanine Proteins 0.000 description 1
- 108010020688 glycylhistidine Proteins 0.000 description 1
- 108010015792 glycyllysine Proteins 0.000 description 1
- 108010081551 glycylphenylalanine Proteins 0.000 description 1
- 108010077515 glycylproline Proteins 0.000 description 1
- 108010087823 glycyltyrosine Proteins 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 108010040030 histidinoalanine Proteins 0.000 description 1
- 108010025306 histidylleucine Proteins 0.000 description 1
- 108010092114 histidylphenylalanine Proteins 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 208000006278 hypochromic anemia Diseases 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000015788 innate immune response Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 108010031424 isoleucyl-prolyl-proline Proteins 0.000 description 1
- 108010078274 isoleucylvaline Proteins 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 108010047926 leucyl-lysyl-tyrosine Proteins 0.000 description 1
- 108010000761 leucylarginine Proteins 0.000 description 1
- 108010057821 leucylproline Proteins 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 description 1
- 229960004999 lycopene Drugs 0.000 description 1
- 235000012661 lycopene Nutrition 0.000 description 1
- 239000001751 lycopene Substances 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 108010064235 lysylglycine Proteins 0.000 description 1
- 108010017391 lysylvaline Proteins 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 108010056582 methionylglutamic acid Proteins 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000012074 organic phase Substances 0.000 description 1
- PIRWNASAJNPKHT-SHZATDIYSA-N pamp Chemical compound C([C@@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(N)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CO)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](C)N)C(C)C)C1=CC=CC=C1 PIRWNASAJNPKHT-SHZATDIYSA-N 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 230000008447 perception Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 108010070409 phenylalanyl-glycyl-glycine Proteins 0.000 description 1
- 108010064486 phenylalanyl-leucyl-valine Proteins 0.000 description 1
- 108010084525 phenylalanyl-phenylalanyl-glycine Proteins 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 108010077112 prolyl-proline Proteins 0.000 description 1
- 108010031719 prolyl-serine Proteins 0.000 description 1
- 108010029020 prolylglycine Proteins 0.000 description 1
- 108010090894 prolylleucine Proteins 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 108010054624 red fluorescent protein Proteins 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 239000012487 rinsing solution Substances 0.000 description 1
- 230000002786 root growth Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 108010048818 seryl-histidine Proteins 0.000 description 1
- 108010026333 seryl-proline Proteins 0.000 description 1
- 108010007375 seryl-seryl-seryl-arginine Proteins 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 201000011549 stomach cancer Diseases 0.000 description 1
- HOWHQWFXSLOJEF-MGZLOUMQSA-N systemin Chemical compound NCCCC[C@H](N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(O)=O)C(=O)OC(=O)[C@@H]1CCCN1C(=O)[C@H]1N(C(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H]2N(CCC2)C(=O)[C@H]2N(CCC2)C(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](NC(=O)[C@H](C)N)C(C)C)CCC1 HOWHQWFXSLOJEF-MGZLOUMQSA-N 0.000 description 1
- 108010050014 systemin Proteins 0.000 description 1
- 229950008418 talipexole Drugs 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- ZCIHMQAPACOQHT-ZGMPDRQDSA-N trans-isorenieratene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/c1c(C)ccc(C)c1C)C=CC=C(/C)C=Cc2c(C)ccc(C)c2C ZCIHMQAPACOQHT-ZGMPDRQDSA-N 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 108010080629 tryptophan-leucine Proteins 0.000 description 1
- 108010084932 tryptophyl-proline Proteins 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
- 108010073969 valyllysine Proteins 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
本发明公开了SlDALR1基因在增强番茄细菌性叶斑病抗性中的应用,该SlDALR1基因的蛋白编码区的核苷酸序列如SEQ ID NO.1所示。本发明通过在普通番茄中过表达SlDALR1基因,发现其增强番茄细菌性叶斑病抗性的新用途,并为培育抗细菌性叶斑病的番茄品种提供了重要的基因资源。
Description
技术领域
本发明涉及植物基因工程技术领域,尤其涉及SlDALR1基因在增强番茄细菌性叶斑病抗性中的应用。
背景技术
番茄(Solanum lycopersicum L.)属于茄科(Solanaceae),番茄属(Solanum),富含糖、食物纤维、矿物质、氨基酸及维生素等营养物质,在平衡人们饮食方面发挥着重要的作用。番茄不仅能降血脂降压,抗真菌,消炎,还能预防胃癌,特别是番茄红素具有抗氧化的功效,在化妆品及制药方面应用广泛。近年来,番茄栽培面积不断增加,对农民增收起着重要的作用,成为世界范围内广泛种植的主要蔬菜之一。
近年来,我国番茄主要以温室和大棚栽培为主,由于温室、大棚内特殊的气候条件和连作种植,番茄易受到各种病害的侵袭。迄今为止已发现200多种病害危害番茄,其中细菌性叶斑病是番茄生产上一种重要的细菌性病害。该病害由丁香假单胞杆菌番茄致病变种(Pseudomonas syringaepv.tomato DC3000)侵染引发。病害的症状包括萎蔫、坏死、褪绿、肿瘤等,一般会导致番茄在内的蔬菜作物减产10%~30%,严重时达50%以上(杨朝阳.“番茄细菌斑点病的发生与防治.”现代农业,2011(5):73-73.),给人类生产和生活造成了很大的经济损失。目前,对番茄细菌性叶斑病的防治仍以化学药剂为主,但是过多使用化学杀菌剂不仅会危及人类身体健康、破坏生态环境,并容易使病菌产生抗药性。实践证明,培育抗病品种是防治病害的一项经济有效的措施。
挖掘重要的抗逆基因,并应用现代分子生物学技术将抗逆基因与其它优良性状聚合,是获得耐逆性强、抗病性强以及高产、优质番茄品种的有效方法之一。对其中的关键基因进行功能研究是开发利用这些基因的前提。
类受体蛋白激酶(Receptor-like protein kinase,RLK)是植物体内普遍存在的一类蛋白激酶,是许多信号识别传递途径中的关键组分。富亮氨酸类受体蛋白激酶(Leucine-rich repeats receptor-like protein kinase,LRR-RLK)是植物中已知的最大的一类跨膜类受体蛋白激酶,在植物生长发育、激素信号转导以及生物与非生物胁迫反应中具有重要的调控功能。典型的LRR-RLK包含胞外LRR结构域、单次跨膜区及胞内激酶结构域(Jiangmei S等.“Genome-wide characterization,evolution,and expressionanalysis of the leucine-rich repeat receptor-like protein kinase(LRR-RLK)genefamily in Rosaceae genomes.”BMC Genomics,2017,18(1):763-777.)。此类蛋白胞外的富亮氨酸结构域识别外界及植物体自身的信号物质,如来自微生物的鞭毛蛋白、系统素等,从而介导生长发育、环境感应和免疫反应等诸多过程(Fan M et al.Diverse roles ofSERK family genes in plant growth,development and defense response.ScienceChina Life Sciences,2016,59(9):889-896.)。研究发现,许多植物的抗病性反应都与LRR-RLK基因有关。例如,十字花科的部分物种具有识别延伸因子Tu(EF-Tu)的模式识别受体EFR(EF-Tu Receptor),EFR是一种典型的LRR-RLK蛋白(Zipfel C等.“Perception ofthe Bacterial PAMP EF-Tu by the Receptor EFR Restricts Agrobacterium-MediatedTransformation.”Cell,2006,125(4):749-760.)。将拟南芥EFR异源转入到番茄中,可以有效提高番茄对青枯病和斑点病等多种病害的广谱抗性,这充分展示了利用不同植物的模式识别受体基因培育广谱、持久抗病农作物的可能性。
目前,在模式植物拟南芥中已经报导过SlDALR1基因同源基因At1G74360(https://www.arabidopsis.org/servlets/TairObjectid=28523&type=locus),该基因命名为GRACE(Germination repression and cell expansion receptor-like kinase),研究发现在拟南芥中的过表达该基因后表现出子叶增大,莲座叶加粗、根系增长等特征(Zhen W等.“Functional and Structural Characterization of a Receptor-LikeKinase Involved in Germination and Cell Expansion in Arabidopsis.”Frontiers in Plant Science,2017,8:1999-2012.)。番茄的SlDALR1基因在抗病方面尚未见相关的研究报导。本发明中发现SlDALR1基因的表达受病原菌诱导,其在受到番茄细菌性叶斑病病原菌侵染时,表达量显著提高。在番茄中过表达SlDALR1基因后,能够有效地增强对细菌性叶斑病的抗性。这些均表明SlDALR1在番茄防御细菌性叶斑病中发挥重要作用。
发明内容
本发明提供了SlDALR1基因(即:Defense-associated LRR-RLK1)在增强番茄细菌性叶斑病抗性中的新用途,为培育抗细菌性叶斑病的番茄品种提供依据。
具体技术方案如下:
本发明提供了SlDALR1基因在增强番茄细菌性叶斑病抗性中的应用,所述SlDALR1基因的编号为solyc06g069650.2.1(PGSB Plant Genome and Systems Biology http://pgsb.helmholtz-muenchen.de/plant/tomato/reportsjsp/sequenceReportCDS.jspelement ID=304839),该基因的蛋白编码区的核苷酸序列如SEQ ID NO.1所示,全基因序列如SEQ ID NO.7所示,其蛋白质编码区长度为3300bp。
SlDALR1基因编码的蛋白为富亮氨酸类受体蛋白激酶,由1099个氨基酸组成,其序列如SEQ ID No.2所示。该蛋白激酶包括胞外LRR结构域、跨膜域和胞内苏氨酸/丝氨酸激酶域3部分,蛋白激酶结构见附图1,其定位于细胞质膜上,见图2。
本发明将SlDALR1基因作为目的基因导入普通番茄‘Condine Red’中得到SlDALR1基因的T0过表达植株,经过连续自交,获得纯合高表达的T2代株系,命名为OE:SlDALR1-1和OE:SlDALR1-4,并将转入pAC004-35S空载体的纯合番茄株系作为对照。与对照株系相比,OE:SlDALR1-1和OE:SlDALR1-4两株系其SlDALR1基因的表达量提高了2000-5000倍。然后,通过将过表达纯合株系OE:SlDALR1-1,OE:SlDALR1-4和对照株系接种番茄细菌性叶斑病菌,2天后分别统计发病率、菌落计数,发现过表达番茄相比对照番茄发病率明显较低,菌落计数发现单位面积叶片上过表达番茄菌落数明显少于对照番茄。综合上述,实验证明了过表达SlDALR1基因能够增强植物对细菌性叶斑病的抗性,表明该基因在植物抗细菌性叶斑病基因工程中具有十分重要的应用价值。
此外,本发明还通过双分子荧光互补(Bimolecular fluorescencecomplementation,BiFC)实验发现,SlDALR1基因编码的蛋白能够与同样位于细胞膜上的SERK3A、SERK3B蛋白互作,形成蛋白复合体,从而在感知病原菌后引发下游的免疫反应;而在前人的研究中已发现,SERK3A/SERK3B通过与多个模式识别受体的互作参与信号识别,通过胞内激酶域不同氨基酸位点的磷酸化修饰不同下游信号作用激发免疫信号(Roux M等.“The Arabidopsis Leucine-Rich Repeat Receptor-Like Kinases BAK1/SERK3 andBKK1/SERK4 Are Required for Innate Immunity to Hemibiotrophic and BiotrophicPathogens.”Plant Cell,2011,23(6):2440-2455.;Heese A等.“The Receptor-likeKinase SERK3/BAK1 Is a Central Regulator of Innate Immunity In Plants.”Proceedings of the National Academy of Sciences,2007,104(29):12217-12222.”)。
所以,进一步地,SlDALR1基因编码的类受体蛋白激酶在增强番茄细菌性叶斑病抗性中的应用,所述类受体蛋白激酶的氨基酸序列如SEQ ID NO.2所示。
进一步地,所述类受体蛋白激酶与共受体蛋白激酶SERK3A/SERK3B结合,形成受体复合体,并在受到番茄细菌性斑点病病原菌的诱导后,共受体蛋白激酶SERK3A/SERK3B将信号向下游传递,进一步引起细胞内的免疫响应,从而增强番茄对细菌性叶斑病的抗性。
进一步地,编码共受体蛋白激酶中SERK3A结构域基因的蛋白编码区的核苷酸序列如SEQ ID NO.3所示;编码共受体蛋白激酶中SERK3B结构域基因的蛋白编码区的核苷酸序列如SEQ ID NO.4所示。
本发明还提供了一种培育抗细菌性叶斑病的番茄的方法,包括以下步骤:
(1)构建含SlDALR1基因的过表达载体;所述SlDALR1基因的蛋白编码区的核苷酸序列如SEQ ID NO.1所示;
(2)将所述过表达载体转入农杆菌感受态细胞中,构建含过表达载体的基因工程菌;
(3)将所述基因工程菌介导转化番茄子叶,培育得到转基因纯合株系。
进一步地,在步骤(1)过表达载体的制备过程中,采用的上游引物如SEQ ID NO.5所示,下游引物如SEQ ID NO.6所示。
进一步地,所述农杆菌为根癌农杆菌GV3101。
与现有技术相比,本发明具有以下有益效果:
(1)本发明通过在普通番茄中过表达SlDALR1基因,发现其增强番茄细菌性叶斑病抗性的新用途,并为培育抗细菌性叶斑病的番茄品种提供了重要的基因资源。
(2)本发明利用转基因技术提供一种抗细菌性叶斑病的番茄种质的选育方法,并获得了抗细菌性叶斑病的转基因纯合植株。
附图说明
图1为SlDALR1基因编码的富亮氨酸类受体蛋白激酶的结构示意图。
图2为实施例4中的SlDALR1基因编码蛋白的亚细胞定位实验,将番茄SlDALR1-GFP和FLS2-mCherry(质膜定位标记)农杆菌工程菌菌液在本塞姆氏烟草(NicotianabenthamianaDomin.)叶片中瞬时共表达;48小时后,使用共聚焦显微镜观察绿色和红色荧光信号;
其中,GFP表示绿色荧光蛋白;Bright表示明场;FLS2-mCherry表示一种用作质膜定位的红色荧光蛋白;Merge表示绿色荧光与红色荧光的融合。
图2中,SlDALR1-GFP的绿色荧光与定位于质膜mCherry的红色荧光重合,表示SlDALR1基因编码的蛋白定位于质膜上。
图3为实施例2中构建的SlDALR1基因T2代过表达纯合株系OE:SlDALR1-1、OE:SlDALR1-4的HA蛋白标签验证;
其中,CK为转入pAC004-35S空载体的对照株系;OE:SlDALR1-1、OE:SlDALR1-4为SLDALR1基因过表达纯合株系。
如图3所示,OE:SlDALR1-1、OE:SlDALR1-4均有目的条带,表明实施例1所构建的pAC004-35S::SlDALR1::HA载体已转入普通番茄中,并在植株中表达。
图4为实施例2中SlDALR1基因在过表达纯合株系的基因表达;
其中,CK为转入pAC004-35S空载体的对照株系;OE:SlDALR1-1、OE:SlDALR1-4为SlDALR1基因过表达纯合株系。
图5为实施例3中番茄SlDALR1基因过表达植株与对照番茄植株接种细菌性叶斑病病原菌60小时之后统计的病情指数;
其中,CK为转入pAC004-35S空载体的对照株系,OE:SlDALR1-1,OE:DALR1-4为SlDALR1基因过表达纯合株系;发病越严重,病级指数越高;对照植株病级指数显著高于过表达纯合株系。
图6为实施例3中番茄SlDALR1基因过表达植株与对照番茄植株接种病原菌72小时后的菌落计数,即将番茄SlDALR1基因过表达植株与对照番茄接种密度为106cfu/mL的细菌性叶斑病病原菌,3天后进行稀释后的菌落计数,数值表示3个重复后的平均值±标准方差;
其中,CK为转入pAC004-35S空载体的对照株系,OE:SlDALR1-1,OE:DALR1-4为SlDALR1基因过表达纯合株系;CFU数值越高,表示发病越严重。
图7为实施例4中双分子荧光互补(Bimolecular fluorescence complementation,BiFC)实验的结果;
其中,YFP为黄色荧光蛋白;p2YN-SlDALR1+p2YC-SERK3A、p2YN-SlD ALR1+p2YC-SERK3B、p2YN-SlDALR1+p2YC、p2YN+p2YC-SERK3A、p2YN+p2YC-SERK3B分别表示两种菌液的混合;p2YN-SlDALR1+p2YC-SERK3A、p2YN-SlDALR1+p2YC-SERK3B为实验组,p2YN-SlDALR1+p2YC、p2YN+p2YC-SERK3A、p2YN+p2YC-SERK3B为对照组;
P2YN-SlDALR1+P2YC-SERK3A/SERK3B观察到荧光,表示SlDALR1与SERK3A/SERK3B存在蛋白互作。
具体实施方式
下面结合具体实施例对本发明作进一步描述,以下列举的仅是本发明的具体实施例,但本发明的保护范围不仅限于此。下述实施例中的实验方法,若未特别指明,实施例中的培养基及实验条件均为常规培养基和实验条件,如分子克隆实验手册(Green M R,Sambrook J.Molecular Cloning:A Laboratory Manual:Three-Volume Set.Cold SpringHarbor Laboratory Pr,2012.),或按照相应实验试剂和仪器说明书建议的条件进行。实施例中所用的试验材料、试剂等,如无特殊说明,均可从商业途径得到。
实施例1番茄SlDALR1基因的克隆和过表达载体的构建
1、番茄总RNA提取
采用Tiangen Plant total RNA extraction kit提取番茄幼嫩叶片的总RNA,其步骤为:
(1)取0.1g叶片在液氮中磨碎,加1mL裂解液,后用匀浆仪处理;
(2)将匀浆样品在15-30℃放置5分钟,使得核酸蛋白复合物完全分离;
(3)4℃,12000转每分钟离心5分钟,去上清,转入一个新的无RNAase的离心管中;
(4)加入200μL氯仿,盖好管盖,剧烈震荡15秒,室温放置3分钟;
(5)4℃,12000转每分钟离心10分钟,样品会分为三层:黄色的有机相,中间层和无色的水相,RNA主要在水相中,水相的体积约为所用裂解液RZ试剂的50%。把水相转移到新管中,进行下一步操作;
(6)缓慢加入50%体积的无水乙醇,混合均匀;将得到的溶液和沉淀一起转入吸附柱CR3,4℃,12000转每分钟离心30秒,弃掉收集管中的废液;
(7)向吸附柱CR3中加入500μL去蛋白液RD,4℃,12000转每分钟离心30秒,弃废液;
(8)向吸附柱CR3中加入600μL漂洗液RW,室温静置2分钟,4℃,12000转每分钟离心30秒,弃废液;
(9)重复操作步骤(8);
(10)将吸附柱放入2mL收集管中,4℃,12000转每分钟离心2分钟,去除残余废液;
(11)将吸附柱CR3转入一个新的离心管中,加入50μL灭菌水,室温放置2分钟,4℃,12000转每分钟离心2分钟,管底即为所提取的RNA;
(12)用(11)所提的RNA用Thermo Fisher公司的反转录试剂盒按说明书进行反转录得到cDNA,存于-20℃保存备用。
2、SlDALR1基因过表达载体的构建
设计番茄SlDALR1基因编码区序列的特异性的扩增引物,引物序列及所携带的酶切位点如下(带有下划线的为酶切位点序列):
SlDALR1-AscI-F:5’-GGGCGCGCCATGTCTGAAGTGGAATCTC-3’;(SEQ ID NO.5)
SlDALR1-PacI-R:5’-CTTAATTAATAATAATGGAGAAGTGCTAC-3’;(SEQ ID NO.6)
以上述从普通番茄品种‘Condine Red’叶片总RNA反转的cDNA(ComplementaryDNA)为模板,通过聚合酶链式反应(Polymerase Chain Reaction,PCR)扩增SlDALR1基因外显子的核苷酸序列,两端带相应的酶切位点。
PCR反应条件为:95℃预变性5分钟;95℃变性15秒,58℃退火15秒,72℃延伸2分钟15秒,共35个循环;72℃延伸5分钟。得到了SlDALR1基因3300bp的片段,进行纯化回收。
回收目的片段:取50μL的PCR扩增产物在质量体积比浓度为0.8%的琼脂糖凝胶中电泳检测,切割目的片段,参照凝胶回收试剂盒(全式金生物技术有限公司)附带的说明书进行操作,得到目的基因片段,连接入过表达载体pAC004(氯霉素抗性),体系为:目的基因片段:过表达载体≈10:1,10μL混匀,于恒温仪中25℃连接2小时,得连接产物。
转化及筛选:从-80℃冰箱中取出大肠杆菌感受态细胞,放置冰上解冻,无菌操作台中将50μL大肠杆菌感受态细胞和5μL的上述连接产物移入1.5mL离心管中,轻轻混匀,冰浴30分钟;打开水浴锅在42℃水浴中热激45秒后立即冰浴2-5分钟,该操作过程中保持离心管绝对静止;在无菌操作台中加入800μL液体LB,放置于37℃摇床上,200转每分钟培养45分钟;4000转每分钟离心1分钟,弃上清,用80μL的液体LB悬浮沉淀,将菌液均匀地涂布于氯霉素抗性的LB平板培养基上,37℃倒置过夜培养12小时至长出单克隆菌落;挑取阳性单克隆送至杭州擎科生物有限公司测序,测序结果如SEQ ID NO:1所示,结果表明,所克隆的序列与PGSB Plant Genome and Systems Biology网站中公布的基因序列(Solyc06g069650.2.1)一致(http://pgsb.helmholtz-muenchen.de/plant/tomato/reportsjsp/sequence Report CDS.jspelement ID=304839)。
3、根癌农杆菌工程菌构建
(1)制备农杆菌感受态细胞
取-80℃保存的农杆菌菌株GV3101于含50μg/mL利发霉素的LB平板划线,28℃培养两天,两天后挑取单菌落接种于1mL含利发霉素25mg·L-1液体LB中,28℃,200转每分钟培养过夜;向50mL离心管中加入50μL菌液和20mL含利发霉素25mg·L-1液体LB中继续培养,至OD600约为0.5;将菌液冰浴30分钟,4℃,5000转每分钟离心5分钟,弃上清液;加入10mL冷的灭菌超纯水使菌液悬浮;4℃,5000转每分钟离心5分钟,弃上清液;加入1mL冷的灭菌超纯水使菌液悬浮,分装(50μL/管),得农杆菌感受态细胞,液氮速冻,-80℃保存。
(2)根癌农杆菌工程菌构建
首先将干净的电转杯置于-20℃预冷待用;取出农杆菌感受态细胞,在冰上解冻,分别向50μL农杆菌感受态细胞菌液中加入2μL的pAC004-35S::SlDALR1过表达载体,轻轻混匀;将混合物转移到已预冷的电转杯的两电极之间,擦干电转杯外壁,置于管槽中,盖上盖子;打开电转仪电源,设置电击参数(2500V,2秒),电击一次;电击完成后,取出电转杯,并加入800μL液体LB混匀,转移到1.5mL离心管中,28℃,200转每分钟培养2-4小时;将菌液于4000转每分钟离心1分钟,倒掉上清液;将剩余约100μL液体LB充分悬浮,并均匀涂布于氯霉素抗性的三抗LB平板培养基(氯霉素、利发霉素、庆大霉素)上,28℃倒置培养2天,2天后,挑取单克隆菌落加入氯霉素抗性的三抗液体LB(氯霉素、利发霉素、庆大霉素)中,28℃,200转每分钟振荡培养24小时,菌液PCR验证得目的条带,即得到含有过表达载体pAC004-35S::SlDALR1::HA的农杆菌工程菌。
实施例2番茄SlDALR1基因的遗传转化与转基因纯合植株的获得
1、农杆菌介导的番茄基因遗传转化
利用含有pAC004-35S::SlDALR1过表达载体的农杆菌工程菌侵染番茄子叶并经过分化培养基和生根培养基的筛选后,对得到的再生植株用pAC004-35S的HA蛋白标签进行检测。
具体的步骤如下:
(1)挑选饱满的普通番茄品种‘Condine Red’种子于清水中,28℃、摇6-8小时,用75%酒精消毒种子30秒,再转入10%次氯酸钠灭菌10-15分钟,最后用灭菌水清洗5-7遍。将灭菌后的种子点播于播种培养基,置于暗处生长3天左右,种子萌芽后,将其转移到光下,25℃培养4天,至子叶完全展开。
(2)切番茄子叶,子叶完全展开而真叶未长出时,用解剖刀将番茄幼苗切下叶尖及下胚轴,每片子叶切成2-3段,将灭菌滤纸放入看护培养基,用镊子小心夹取切好的子叶,轻置于看护培养基上,黑暗下看护过夜,次日用含上述构建好的含pAC004-35S::SlDALR1::HA载体的农杆菌工程菌侵染子叶,时间为3分钟,侵染后用灭菌滤纸擦干残留菌液并将子叶放回培养基,暗培养2天。
(3)子叶与农杆菌在看护培养基共同培养2天后,将番茄子叶转入2Z分化培养基中(潮霉素抗性,诱导愈伤组织),筛选抗性愈伤组织,约15天后转入0.2Z分化培养基(潮霉素抗性,诱导发芽),之后每隔15天左右转入新的0.2Z分化培养基。
(4)当在愈伤上生出小苗后,转入生根培养基进行生根培养20-30天,直至根系发育完全,待根系发育良好后,且长势较好时,打开培养瓶盖往瓶内稍稍倒入少量灭菌水保湿,套上一透明的塑料袋进行炼苗,得到T0代过表达番茄苗。
2、过表达材料验证
(1)取上述T0代过表达苗叶片少许,放入加有小钢珠的2mL离心管中,用液氮冷冻,研磨仪粉碎样品30秒后,分别向离心管中加入100μL蛋白上样缓冲液和2μL的巯基乙醇,颠倒混匀放入95℃水浴锅中变性5-10分钟,期间轻轻上下颠倒几次。
(2)将(1)中的变性蛋白质样品4℃,12000转每分钟离心10分钟,离心后取上清液15μL上样,200V电压下,待蛋白条带分开后,随即依次转膜、脱脂奶粉封闭、孵育一抗(Anti-HA)、二抗(Anti-mouse IgG,Antibody),最后,用化学发光仪曝光具有正确大小蛋白条带的植株即为T0代SlDALR1基因过表达植株。
3、过表达纯合株系的获得
将T0代转基因过表达植株自交分别得到T1代种子,从每个T0代过表达植株所产生的T1代中取6-9个阳性株继续自交产生T2代并进行分离分析。当T1代阳性株产生的T2代幼苗经过检测全部为阳性株,则该T1代植株为过表达纯合株;反之,则为杂合株。
将上述验证纯合的T2代转基因植株提取总RNA,具体步骤如上,用Thermo Fisher公司的反转录试剂盒进行反转录得到cDNA,并以之为模板,以SlDALR1基因特异性定量引物进行检测,用ACTIN基因作为内参基因,利用实时荧光定量PCR方法检测SlDALR1基因在不同株系中的表达量。结果如图4所示,SlDALR1过表达纯合株系OE:SlDALR1-1、OE:SlDALR1-4表达量与对照株系相比升高约2000-5000倍。OE:SlDALR1-1、OE:SlDALR1-4纯合株系的HA蛋白标签验证结果如图3所示,具体做法如上。
以下实施例3均以OE:SlDALR1-1、OE:SlDALR1-4为实验材料。
所用引物如下:
q-ACTIN-F:5’-TGTCCCTATTTACGAGGGTTATGC-3’(SEQ ID NO.8)
q-ACTIN-R:5’-CAGTTAAATCACGACCAGCCCGAT-3’(SEQ ID NO.9)
q-SlDALR1-F:5’-CTCTATGGGTGGTGCCTTGA-3’(SEQ ID NO.10)
q-SlDALR1-R:5’-CTCTGTGGACAATGCAAGGG-3’(SEQ ID NO.11)
实施例3番茄细菌性叶斑病抗性试验
对实施例2所得番茄SlDALR1过表达纯合株系OE:SlDALR1-1、OE:SlDALR1-4进行接种细菌性叶斑病病原菌处理。
1、番茄细菌性叶斑病病原菌的准备
将番茄细菌性叶斑病病原菌菌种涂布于含25mg/L利福平的固体KB(King’s B)培养基(蛋白胨10g,K2HPO4 1.5g,甘油15mL,琼脂15g,无菌水1L)上于28℃培养箱中培养2天后得以活化,挑取单菌落于含有同样抗生素的液体培养基中28℃,以200转每分钟离心,扩大培养8-16小时,直至OD600=0.8-1.0,然后于2500转每分钟离心10分钟,收集菌体。将收集的菌体用10mM MgCl2溶液重悬,并调节细菌浓度至OD600约为0.1,加0.03%有机硅,准备对过表达与对照番茄植株进行喷施。
2、细菌性叶斑病病原菌接种
分别选取株系OE:SlDALR1-1、OE:SlDALR1-4的T2代纯合植株各8株和对照植株8株,进行病原菌接种实验,对过表达与对照番茄植株的叶背进行同样地喷施等量上述所准备的病原菌,至菌液浸润所有叶片背部,细菌悬浮液通过气孔进入细胞间隙。然后,置于70%湿度环境中有利于病原菌的发育,2-3天后观察植株发病情况,统计发病率,并进行菌落计数。
3、病情指数统计
接种番茄细菌性叶斑病病原菌60小时后观察发病症状,并统计发病叶片数。发病症状按轻重程度依次为0、Ⅰ、Ⅱ、Ⅲ、Ⅳ五个等级,分级标准为:0级,叶片正常;Ⅰ级,叶片下表皮可见少数病斑;Ⅱ级,叶片下表皮局部密集病斑;Ⅲ级,叶片下表皮多部位密集病斑,但未散布整片叶;Ⅳ级,叶片下表皮全片叶可见病斑分布。同时每个处理至少统计50片番茄叶片。病情指数的计算公式如下:
病情指数=∑(各级叶片数×发病级别)×100/(总叶片数×最高发病级数)统计结果如图5所示,相比于对照番茄,两个过表达番茄株系OE:SlDALR1-1和OE:SlDALR1-4发病症状明显减轻。
4、菌落计数
采用平板稀释法对病原菌的菌落形成单位(Colony-Forming Units,CFU)值进行统计。对接种病原菌72小时过表达番茄株系OE:SlDALR1-1和OE:SlDALR1-4与对照番茄的叶片用直径为5mm的打孔器获得相同大小的叶盘,将叶盘样品放入2mL离心管中,在无菌条件下,将叶片用75%无水乙醇浸泡2分钟,然后无菌水冲洗3次,将叶盘转入新的无菌的2mL离心管中,加入100μL的10mM MgCl2溶液将叶盘研磨匀浆,磨匀后进行10倍、100倍、1000倍、10000倍的梯度稀释。各取10μL稀释液涂布于含25mg/L利福平的固体KB培养基上,28℃培养2天后计数菌落数量。每个过表达株系与作为对照番茄各3次重复,试验数据用SPSS软件进行统计分析。
结果如图6所示,菌落计数的结果表明两个过表达株系单位面积叶片上的病原菌菌落数量明显少于对照番茄,即发病程度明显减轻。
综合上述实验,结果均说明番茄SlDALR1基因在植株中过表达后对番茄细菌性叶斑病具有显著的抗性作用。上述实验设有三次重复,三次实验结果基本一致。
实施例4 SlDALR1亚细胞定位及SlDALR1与SERK3A/SERK3B的蛋白互作验证
1、SlDALR1的蛋白亚细胞定位
将含SlDALR1::GFP的重组植物表达载体的农杆菌和FLS2-mCherry(FLS2为已知的质膜定位标记基因)农杆菌在本塞姆氏烟草(Nicotiana benthamianaDomin.)叶片中瞬时过表达,2天后,检测绿色荧光与红色荧光的发光情况。
具体步骤如下:
(1)构建载体:同实施例1将SlDALR1基因的外显子序列构建到含报告基因GFP的植物表达载体pAC402-35S中;
所用引物序列及所携带的酶切位点如下(带有下划线的为酶切位点序列):
SlDALR1-XhoI-F:5’-CCCTCGAGCATGTCTGAAGTGGAATCTC-3’;(SEQ ID NO.12)
SlDALR1-SacI-R:5’-CGAGCTCTAAATGGAGAAGTGCTACGAC-3’。(SEQ ID NO.13)
(2)将上述构建的pAC402-35S::SlDALR1::GFP质粒转入农杆菌工程菌中,并将该农杆菌工程菌菌液于28℃,200转每分钟过夜扩摇;
(3)当菌液OD600值介于0.8-1.0之间时,1000转每分钟离心5分钟收集农杆菌;
(4)用10mL灭菌水轻柔重悬农杆菌,然后再次离心收集菌液;重复以上步骤,进一步去除残留的抗生素;
(5)用农杆菌侵染缓冲液将菌体重悬,调OD600至约1.0,室温放置2-3小时得到侵染菌液;
(6)侵染前,将上述菌液与用于质膜定位的FLS2-mCherry农杆菌菌液等体积混匀;
(7)用针头在烟草叶片背面上避开叶脉扎孔,用无针头注射器将侵染菌液从扎孔处注射至叶片内;
(8)2天后,从注射部位取1-2cm2叶片。在激光扫描共聚焦显微镜下观察绿色及红色荧光。
定位结果如图2所示,SlDALR1-GFP的绿色荧光与定位于质膜mCherry的红色荧光重合,表示:SlDALR1编码的蛋白定位于质膜上。
2、SlDALR1与SERK3A/SERK3B的蛋白互作鉴定
为探究SlDALR1和共受体SERK3A/SERK3B之间的关系,本发明采用了BiFC进行了SlDALR1和SERK3A/SERK3B所编码的类受体激酶的蛋白互作验证;将含有荧光蛋白YFP(Yellow Fluorescent Protein,YFP)N端和C端的BiFC载体在本塞姆氏烟草(NicotianabenthamianaDomin.)叶片中瞬时过表达,2天后,在共聚焦显微镜下观察YFP发光情况。
具体步骤如下:
(1)构建载体,同实施例1将SlDALR1基因外显子的核苷酸序列连接到p2YN,SERK3A/SERK3B基因外显子的核苷酸序列构建到p2YC的表达载体中(p2YN、p2YC为BiFC所用载体,分别含YFP的N端、C端),并确定其是可以在本塞姆氏烟草叶中表达的。
(2)将(1)中构建好的载体质粒转化到农杆菌工程菌中,菌株为GV3101,将该菌液于28℃,200转每分钟过夜扩摇;
(3)当菌液OD600值介于0.8-1.0之间时,1000转每分钟离心5分钟收集农杆菌;
(4)用10mL灭菌水轻柔重悬农杆菌,然后再次离心收集菌体;
(5)重复步骤4,进一步去除残留的抗生素;
(6)用农杆菌侵染缓冲液将菌体重悬,调OD600至1.0,室温放置2-3小时得到侵染菌液;
(7)用针头在烟草叶片背面上避开叶脉扎孔,用无针头注射器将菌液从扎孔处注射至叶片内;
(8)2天后,从注射部位取1-2cm2叶片。在激光扫描共聚焦显微镜下观察黄色荧光。
结果如图7所示,p2YN-SlDALR1+p2YC-SERK3A/SERK3B共注射2天后,均可观察到荧光,而另外的三个对照组均无荧光。
实施例4所用的本塞姆氏烟草(Nicotiana benthamianaDomin.)为在16小时-光/8小时-暗条件的温室中生长5周左右的烟草植株。重悬农杆菌所用的缓冲液为:10mM MES-KOH(pH=5.6),10mM MgCl2,150μM乙酰丁香酮(乙酰丁香酮溶解在二甲亚砜中,-20℃储存)。
序列表
<110> 浙江大学
<120> SIDALR1基因在增强番茄细菌性叶斑病抗性中的应用
<160> 13
<170> SIPOSequenceListing 1.0
<210> 1
<211> 3300
<212> DNA
<213> 番茄(Solanum lycopersicum L.)
<400> 1
atgtctgaag tggaatctca tgtttttctt cccattgtat tactcaattt cttcattctc 60
ataacaggta ggcttgtttg tggagactca attgagacag acaagcagtt gctgctgaac 120
ttgaagtcat ttcttaagga acaaaatcct gttgacaaag gattcaaata taaccattgg 180
aatcctacag atttgtcacc ctgtagatgg cctggaatat catgcactac ttccatcaat 240
cgtgtcactg gaatcgatct ctcggaaagt aatctggctg ggaagttgtt taataacttc 300
tcagccatga cagaattgaa ctctcttgac ttgtccaaga acacattttc agagtccatt 360
ccatcagact taggctggtg tagaaatctg aagttcttga acttgtcaca caatatcatt 420
gttggtgacc tcaacttgac aggcctgaac aagctggaag ttcttgattt gacaatgaac 480
aggattcatg ggctaacgat ccctgagatt tgtgataact tagctgttgc aaatatttct 540
aacaacaatt tcactagtga gagtggattt gaattttctc actgcaagaa actgaagtat 600
cttgatctaa gctacaatta tttgacaggg aatctgtcgt tcgggcttga tatgttgaat 660
atgttttcag catctcataa caacttaagt ggctctctgc cttcctggat tttcactcaa 720
aactgctctt tgcaaggttt ggacttatca gaaaataggt tctttggtga attgcctaca 780
tctatcgcga actgtaagcg attagtagag ttgaatttgt ggggaaatag cttttcaggg 840
tcaatcccta gagggattgg atcagtacat agtcttaaag aactttgctt gggaagtaac 900
aacttttcaa gtgatgttcc agatactcta tcaggcctaa acaaattggt atttctggac 960
ctaagtagaa acaactttgg aggagaaata caagaaattt tcgggcaact gacacaggta 1020
agatttctcg tgctgcacgg gaactcatat actggaggca tagtatcatc aggaattcca 1080
aacttggtga acctttcgcg gttggactta agtgataacc acttctctgg tccattacca 1140
gttgaaattt ctgagatgaa aggtttggag tttttgattc ttgcacataa ccagtttagt 1200
ggcaatatac cttcagaata tggagatctt acagcacttc aggctgttga tctttcctct 1260
aataggatca atggttcaat accaccaagt ttcgggaagc taaggtcact tttgtggttg 1320
atgcttgcaa acaattcatt gagtggtgaa atcccatcag agttggggaa ctgcagcagc 1380
ttgttatggt tgaatctcgc gaataatcaa cttactggtc caattcctcc tcaattagcg 1440
agtattggtg cagatccaat gctaacattt ttgttgaata ggggaaagga gaagctcact 1500
gctagcccag gggattgttt tgccatgagg aggtggatac cagctgacta ccctccattt 1560
agctttatat atcctctatt gacagggaag agttgtagaa tcctggggga taagttgttt 1620
atgggagatg gtttaatgcc actctgtgaa cctggtagta atgtacgaaa gaatcaggta 1680
ccgggctata ttcaacttag tgataacaaa ttgtctggtg agatccctcc tgagattagc 1740
aacatgaaga aaatgagtat gatgcatttg ggtgcaaatg aattttctgg caggctccct 1800
tcagagatcg ggcaactgca cctagtagtc cttaatgttt cacagaataa attttctggt 1860
gaaatcccaa agcagattgg ccatcttaag tgcttgctaa accttgacct ttcatttaac 1920
aatttttctg gtccattccc agttagcttc agtaacttgc atgatttgag caagtttaac 1980
atctcttaca accaatacat ctatggagct gtaccagaaa ccgggcaact gcttactttt 2040
gataagtcat catttcttgg taatccactg ttacgtcttc catccttcat gcacaactct 2100
aagaacaaca cagaacgaaa cacgaatgac actcacaaaa agcccaaaaa ggtgggtgca 2160
cttttggtaa ttgtggttct ggtactagcc ttcctagtct gtggagtcat gtcccttgtt 2220
gtctgcctcc ttataaaagt gccaaggggt tccgcgggaa tcttactgga ggatacagag 2280
ggcagacatg attctccatc aagtactaat gcatcctcat cacggggttc tgatgatgtt 2340
aaggttattc gtttggacag aacaagcttc acacattctg atatactgaa ggccacctgg 2400
aacttctcga atgatagaat tatcggtagg ggaggattcg ggatagtcta tcgtggagtc 2460
ttgcctgatg gaagggacgt agcagtgaag aagctacaga gggagggaat tgaaggagaa 2520
agagagttca gagctgaaat ggaggcgctc agtgggaatg gctcgggttg gcctcatccc 2580
aaccttgtaa ctctctatgg gtggtgcctt gatggatcag agaaacttct agtctatgag 2640
tacatggaag gtggtacctt agaggatttc attacagatc gcacaaggtt tacatggaaa 2700
tgccgaattc aagcagcaat cgatgtagca cgtgctttag tctacttgca ccatgattgc 2760
tacccttgca ttgtccacag agatgtcaaa gctaacaacg tgcttcttga caaggacgga 2820
agagcaaaag tcaccgattt tggccttgcc agggtcatga tttctgaaca tactcatgtt 2880
agcacaatgg tggcagggac tattggttat gttgcaccag aatatgggca gataatgcag 2940
gccactacaa aaggagatgt ctacagctat ggggtgctag caatggagct agcaactggg 3000
aggcatgcta tagatggggg cgaagaatgt ctagttgaat gggcgacaag ggtcatggga 3060
gacggaagga aagggttcac cagagccatt ataccagatg ctctattggt acctggccta 3120
gtagagggag cagaggaaat gtatgagttg cttaggattg ggataaggtg cacagctgag 3180
acgcctcatg accggcctaa catgaagcag gtattagata tgttgattag tgttcctagc 3240
agccaaaagg gatccagccg tagctttgga tcaagtcgta gcacttctcc attattatga 3300
<210> 2
<211> 1099
<212> PRT
<213> 番茄(Solanum lycopersicum L.)
<400> 2
Met Ser Glu Val Glu Ser His Val Phe Leu Pro Ile Val Leu Leu Asn
1 5 10 15
Phe Phe Ile Leu Ile Thr Gly Arg Leu Val Cys Gly Asp Ser Ile Glu
20 25 30
Thr Asp Lys Gln Leu Leu Leu Asn Leu Lys Ser Phe Leu Lys Glu Gln
35 40 45
Asn Pro Val Asp Lys Gly Phe Lys Tyr Asn His Trp Asn Pro Thr Asp
50 55 60
Leu Ser Pro Cys Arg Trp Pro Gly Ile Ser Cys Thr Thr Ser Ile Asn
65 70 75 80
Arg Val Thr Gly Ile Asp Leu Ser Glu Ser Asn Leu Ala Gly Lys Leu
85 90 95
Phe Asn Asn Phe Ser Ala Met Thr Glu Leu Asn Ser Leu Asp Leu Ser
100 105 110
Lys Asn Thr Phe Ser Glu Ser Ile Pro Ser Asp Leu Gly Trp Cys Arg
115 120 125
Asn Leu Lys Phe Leu Asn Leu Ser His Asn Ile Ile Val Gly Asp Leu
130 135 140
Asn Leu Thr Gly Leu Asn Lys Leu Glu Val Leu Asp Leu Thr Met Asn
145 150 155 160
Arg Ile His Gly Leu Thr Ile Pro Glu Ile Cys Asp Asn Leu Ala Val
165 170 175
Ala Asn Ile Ser Asn Asn Asn Phe Thr Ser Glu Ser Gly Phe Glu Phe
180 185 190
Ser His Cys Lys Lys Leu Lys Tyr Leu Asp Leu Ser Tyr Asn Tyr Leu
195 200 205
Thr Gly Asn Leu Ser Phe Gly Leu Asp Met Leu Asn Met Phe Ser Ala
210 215 220
Ser His Asn Asn Leu Ser Gly Ser Leu Pro Ser Trp Ile Phe Thr Gln
225 230 235 240
Asn Cys Ser Leu Gln Gly Leu Asp Leu Ser Glu Asn Arg Phe Phe Gly
245 250 255
Glu Leu Pro Thr Ser Ile Ala Asn Cys Lys Arg Leu Val Glu Leu Asn
260 265 270
Leu Trp Gly Asn Ser Phe Ser Gly Ser Ile Pro Arg Gly Ile Gly Ser
275 280 285
Val His Ser Leu Lys Glu Leu Cys Leu Gly Ser Asn Asn Phe Ser Ser
290 295 300
Asp Val Pro Asp Thr Leu Ser Gly Leu Asn Lys Leu Val Phe Leu Asp
305 310 315 320
Leu Ser Arg Asn Asn Phe Gly Gly Glu Ile Gln Glu Ile Phe Gly Gln
325 330 335
Leu Thr Gln Val Arg Phe Leu Val Leu His Gly Asn Ser Tyr Thr Gly
340 345 350
Gly Ile Val Ser Ser Gly Ile Pro Asn Leu Val Asn Leu Ser Arg Leu
355 360 365
Asp Leu Ser Asp Asn His Phe Ser Gly Pro Leu Pro Val Glu Ile Ser
370 375 380
Glu Met Lys Gly Leu Glu Phe Leu Ile Leu Ala His Asn Gln Phe Ser
385 390 395 400
Gly Asn Ile Pro Ser Glu Tyr Gly Asp Leu Thr Ala Leu Gln Ala Val
405 410 415
Asp Leu Ser Ser Asn Arg Ile Asn Gly Ser Ile Pro Pro Ser Phe Gly
420 425 430
Lys Leu Arg Ser Leu Leu Trp Leu Met Leu Ala Asn Asn Ser Leu Ser
435 440 445
Gly Glu Ile Pro Ser Glu Leu Gly Asn Cys Ser Ser Leu Leu Trp Leu
450 455 460
Asn Leu Ala Asn Asn Gln Leu Thr Gly Pro Ile Pro Pro Gln Leu Ala
465 470 475 480
Ser Ile Gly Ala Asp Pro Met Leu Thr Phe Leu Leu Asn Arg Gly Lys
485 490 495
Glu Lys Leu Thr Ala Ser Pro Gly Asp Cys Phe Ala Met Arg Arg Trp
500 505 510
Ile Pro Ala Asp Tyr Pro Pro Phe Ser Phe Ile Tyr Pro Leu Leu Thr
515 520 525
Gly Lys Ser Cys Arg Ile Leu Gly Asp Lys Leu Phe Met Gly Asp Gly
530 535 540
Leu Met Pro Leu Cys Glu Pro Gly Ser Asn Val Arg Lys Asn Gln Val
545 550 555 560
Pro Gly Tyr Ile Gln Leu Ser Asp Asn Lys Leu Ser Gly Glu Ile Pro
565 570 575
Pro Glu Ile Ser Asn Met Lys Lys Met Ser Met Met His Leu Gly Ala
580 585 590
Asn Glu Phe Ser Gly Arg Leu Pro Ser Glu Ile Gly Gln Leu His Leu
595 600 605
Val Val Leu Asn Val Ser Gln Asn Lys Phe Ser Gly Glu Ile Pro Lys
610 615 620
Gln Ile Gly His Leu Lys Cys Leu Leu Asn Leu Asp Leu Ser Phe Asn
625 630 635 640
Asn Phe Ser Gly Pro Phe Pro Val Ser Phe Ser Asn Leu His Asp Leu
645 650 655
Ser Lys Phe Asn Ile Ser Tyr Asn Gln Tyr Ile Tyr Gly Ala Val Pro
660 665 670
Glu Thr Gly Gln Leu Leu Thr Phe Asp Lys Ser Ser Phe Leu Gly Asn
675 680 685
Pro Leu Leu Arg Leu Pro Ser Phe Met His Asn Ser Lys Asn Asn Thr
690 695 700
Glu Arg Asn Thr Asn Asp Thr His Lys Lys Pro Lys Lys Val Gly Ala
705 710 715 720
Leu Leu Val Ile Val Val Leu Val Leu Ala Phe Leu Val Cys Gly Val
725 730 735
Met Ser Leu Val Val Cys Leu Leu Ile Lys Val Pro Arg Gly Ser Ala
740 745 750
Gly Ile Leu Leu Glu Asp Thr Glu Gly Arg His Asp Ser Pro Ser Ser
755 760 765
Thr Asn Ala Ser Ser Ser Arg Gly Ser Asp Asp Val Lys Val Ile Arg
770 775 780
Leu Asp Arg Thr Ser Phe Thr His Ser Asp Ile Leu Lys Ala Thr Trp
785 790 795 800
Asn Phe Ser Asn Asp Arg Ile Ile Gly Arg Gly Gly Phe Gly Ile Val
805 810 815
Tyr Arg Gly Val Leu Pro Asp Gly Arg Asp Val Ala Val Lys Lys Leu
820 825 830
Gln Arg Glu Gly Ile Glu Gly Glu Arg Glu Phe Arg Ala Glu Met Glu
835 840 845
Ala Leu Ser Gly Asn Gly Ser Gly Trp Pro His Pro Asn Leu Val Thr
850 855 860
Leu Tyr Gly Trp Cys Leu Asp Gly Ser Glu Lys Leu Leu Val Tyr Glu
865 870 875 880
Tyr Met Glu Gly Gly Thr Leu Glu Asp Phe Ile Thr Asp Arg Thr Arg
885 890 895
Phe Thr Trp Lys Cys Arg Ile Gln Ala Ala Ile Asp Val Ala Arg Ala
900 905 910
Leu Val Tyr Leu His His Asp Cys Tyr Pro Cys Ile Val His Arg Asp
915 920 925
Val Lys Ala Asn Asn Val Leu Leu Asp Lys Asp Gly Arg Ala Lys Val
930 935 940
Thr Asp Phe Gly Leu Ala Arg Val Met Ile Ser Glu His Thr His Val
945 950 955 960
Ser Thr Met Val Ala Gly Thr Ile Gly Tyr Val Ala Pro Glu Tyr Gly
965 970 975
Gln Ile Met Gln Ala Thr Thr Lys Gly Asp Val Tyr Ser Tyr Gly Val
980 985 990
Leu Ala Met Glu Leu Ala Thr Gly Arg His Ala Ile Asp Gly Gly Glu
995 1000 1005
Glu Cys Leu Val Glu Trp Ala Thr Arg Val Met Gly Asp Gly Arg Lys
1010 1015 1020
Gly Phe Thr Arg Ala Ile Ile Pro Asp Ala Leu Leu Val Pro Gly Leu
1025 1030 1035 1040
Val Glu Gly Ala Glu Glu Met Tyr Glu Leu Leu Arg Ile Gly Ile Arg
1045 1050 1055
Cys Thr Ala Glu Thr Pro His Asp Arg Pro Asn Met Lys Gln Val Leu
1060 1065 1070
Asp Met Leu Ile Ser Val Pro Ser Ser Gln Lys Gly Ser Ser Arg Ser
1075 1080 1085
Phe Gly Ser Ser Arg Ser Thr Ser Pro Leu Leu
1090 1095
<210> 3
<211> 1848
<212> DNA
<213> 番茄(Solanum lycopersicum L.)
<400> 3
atggatcagt cggtgttggc gatctgggta tttctctgct taattggtct gcttttcaat 60
ttgtcacccg tcgccggtaa cgctgaaggt gatgccttga atgctctgaa gacaaatttg 120
gctgatccta atagtgttct acagagttgg gatgcaaccc ttgttaatcc ttgtacttgg 180
ttccatgtga catgcaacaa tgaaaatagt gtgactagag ttgatctagg aaatgcaaat 240
ctatcaggtc aactggtacc acagcttggc caactccaga aattgcagta cttggaactt 300
tatagtaata acataagcgg aagaattcca aatgaactgg gaaacttgac agagttggtt 360
agtttggatc tttacctgaa caacttaaat ggtcctattc ctccctcatt gggcaggctt 420
cagaagctac gcttcctgag gctcaataat aacagtttga atgaaggtat tcccatgtct 480
ctaaccacca ttgttgcact tcaagtactt gatctctcaa acaaccattt gacaggacta 540
gttccagtca acggttcctt ttcacttttt actcctataa gttttgctaa taatcagttg 600
gaagttcctc cagtttctcc acctcctcct cttcctccta caccctcatc gtcatcttca 660
gtgggcaaca gcgcaactgg agctatcgct ggaggagttg ctgcaggcgc tgctcttcta 720
tttgcagctc ctgcaatttt tcttgcttgg tggcgtcgga ggaaaccaca agaccacttc 780
tttgatgttc ctgctgagga ggatccagaa gttcatctgg gacaactcaa aaggttttcc 840
ttgcgtgaac tacaagttgc gtcggataat tttagcaaca gaaatatact cggtagaggt 900
ggatttggta aggtttataa gggccggtta gctgatggct ctttagttgc agtgaaaaga 960
ctaaaagagg aacgtactca aggtggagag ttacagttcc agacagaagt agaaatgatc 1020
agcatggctg tacaccgaaa cctacttcgt ttacggggct tttgcatgac acccactgag 1080
cgcgtgcttg tttatcctta catggagaat ggaagtgttg catcacgttt aagagagagg 1140
cctgaatcag agcccccact tgactggcca aaaaggaagc gtattgcact tggatctgca 1200
agaggccttg cttacttgca tgatcattgt gatcctaaaa ttattcatcg tgacgtcaaa 1260
gccgcaaata tcttgttgga tgaggagttt gaagcagttg ttggggattt tgggttagct 1320
aaactcatgg actacaagga tactcatgtt accactgctg tacgtggtac aattgggcat 1380
attgcccctg aatatttatc tactggtaaa tcttctgaga aaactgatgt ctttggctat 1440
ggggttatgc ttctagagct cataactggg caaagggctt ttgatcttgc tcgacttgcg 1500
aatgatgatg atgtcatgct gctagattgg gtgaagggac tcctgaagga caagaaatat 1560
gaaacattag ttgatgcaga tcttcaaggt aattacaatg aagaagaggt ggaacaactt 1620
attcaggtag ctctactttg cacgcagagt acgcctacgg aacgtccaaa gatgtcagaa 1680
gttgtaagaa tgcttgaagg tgatggcctt gctgagaggt gggaggaatg gcaaaaggag 1740
gagatgttcc ggcaagatta caaccatgta caccaccccc atactgattg gataatagct 1800
gactccacgt caaatatccg accggatgag ttgtcagggc caagatga 1848
<210> 4
<211> 1854
<212> DNA
<213> 番茄(Solanum lycopersicum L.)
<400> 4
atgatggatc aatgggtctt ggggatcttg gggtctgctt cagtttttct ttgcttaatt 60
gggctgttgc tggttcctgt atatggtaac actgaaggtg atgcgttgaa tgcgctgaag 120
acaaccttgg ctgatcctaa caatgtttta cagagttggg atccgaccct tgtcaatcct 180
tgtacttggt ttcatgtgac ctgcaacagt gagaatagcg taactagagt tgatcttgga 240
aatgcaaatt tgtcgggtca actcgttccc cagcttggtc aactctcgaa tttgcaatac 300
ttggaacttt atagtaataa tataagcgga agaattcctt atgaactggg gaatttgacg 360
aatttggtga gcttggatct ttacctgaac aagttagttg gtccaatccc cgacactttg 420
ggcaagcttc agaaactacg tttcctgagg cttaacaata acagtttgac tggacaaatt 480
ccggtgcttt taaccacagt cacttcactt caagtgcttg atttgtcaaa caataaatta 540
acaggacctg ttccagtcaa tggttccttc tcgcttttta ctcctatcag ttttgcgaat 600
aatccattgg aaactcctcc agtttctcca cctccaccta ttactccaac acccacttct 660
ccaggtgtgg gaaacagtgc aaccggggcg atcgctggcg gtgttgctgc aggggctgct 720
cttctgtttg cagctcctgc gattttgctt gcttggtggc gtcgaaggaa accggaagac 780
cacttctttg atgttccagc tgaggaggat ccagaagttc atcttggaca actcaagagg 840
ttctccttgc gcgaattaca agttgcaact gacaatttta gcaacaaaaa tatactcgga 900
agaggaggat ttggcaaggt ttacaagggc aggctggctg atggctcttt agttgctgtt 960
aaaagattaa aagaggagcg tactcaaggc ggagagttac agtttcagac agaagttgaa 1020
atgattagta tggccgtgca tcgaaatcta cttcgtttat ggggcttttg catgacagca 1080
actgaacggt tgcttgttta tccatatatg gcaaatggaa gtgttgcttc acgcttgaga 1140
gagcggccag aatcagatcc accacttggc tggcctataa ggaagtgtat agcacttgga 1200
tctgcaagag gccttgctta tttgcatgat cattgcgacc ctaagatcat ccaccgtgat 1260
gtcaaagctg caaatatatt gttggatgag gagtatgaag cagttgttgg ggattttggg 1320
ttagccaaac tcatggacta caaggatact catgttacta ctgctgtacg tggtacaatt 1380
gggcatatcg cccccgagta tttatccacc ggtaaatctt cagagaaaac tgatgtgttt 1440
ggctatggag ttatgcttct agagctcata actgggcaaa gggcatttga tcttgctcga 1500
cttgcaaatg atgatgatgt catgttgcta gattgggtca agggacttct aaaggacgag 1560
aagtatgaaa cattagtaga tgcagatctt cagggtaatt ataatgaaga agaggtgaaa 1620
cagcttattc aggtagctct actctgcaca cagagctctc ctatggaacg tccaaagatg 1680
tcggaagtgg tcagaatgct tgaaggtgat ggccttgccg agaggtggga agaatggcag 1740
aaggaagaga tgttccgcca agacttcaac catgctcacc atcctcacac tgattggatt 1800
attgcagatt ccacttataa tctacgaccc gatgagttat cagggccaag atga 1854
<210> 5
<211> 28
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 5
gggcgcgcca tgtctgaagt ggaatctc 28
<210> 6
<211> 29
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 6
cttaattaat aataatggag aagtgctac 29
<210> 7
<211> 3562
<212> DNA
<213> 番茄(Solanum lycopersicum L.)
<400> 7
atgtctgaag tggaatctca tgtttttctt cccattgtat tactcaattt cttcattctc 60
ataacaggta tgtatgtata tatatatata tcctcatttc atatttgata gctttaattt 120
gatcaagttt agtagtagag tctcattctg tacataatta aaaagattca attttttaaa 180
caacatttaa tgcagcaaga cagatttttt tatttttaac ttagcaaaat cataagacca 240
gtcttgtttg tgttactcca aacaccaagc attaaatgtt gttctgtttt gtttttggga 300
gaattttgac ttgtatttat attatatagg taggcttgtt tgtggagact caattgagac 360
agacaagcag ttgctgctga acttgaagtc atttcttaag gaacaaaatc ctgttgacaa 420
aggattcaaa tataaccatt ggaatcctac agatttgtca ccctgtagat ggcctggaat 480
atcatgcact acttccatca atcgtgtcac tggaatcgat ctctcggaaa gtaatctggc 540
tgggaagttg tttaataact tctcagccat gacagaattg aactctcttg acttgtccaa 600
gaacacattt tcagagtcca ttccatcaga cttaggctgg tgtagaaatc tgaagttctt 660
gaacttgtca cacaatatca ttgttggtga cctcaacttg acaggcctga acaagctgga 720
agttcttgat ttgacaatga acaggattca tgggctaacg atccctgaga tttgtgataa 780
cttagctgtt gcaaatattt ctaacaacaa tttcactagt gagagtggat ttgaattttc 840
tcactgcaag aaactgaagt atcttgatct aagctacaat tatttgacag ggaatctgtc 900
gttcgggctt gatatgttga atatgttttc agcatctcat aacaacttaa gtggctctct 960
gccttcctgg attttcactc aaaactgctc tttgcaaggt ttggacttat cagaaaatag 1020
gttctttggt gaattgccta catctatcgc gaactgtaag cgattagtag agttgaattt 1080
gtggggaaat agcttttcag ggtcaatccc tagagggatt ggatcagtac atagtcttaa 1140
agaactttgc ttgggaagta acaacttttc aagtgatgtt ccagatactc tatcaggcct 1200
aaacaaattg gtatttctgg acctaagtag aaacaacttt ggaggagaaa tacaagaaat 1260
tttcgggcaa ctgacacagg taagatttct cgtgctgcac gggaactcat atactggagg 1320
catagtatca tcaggaattc caaacttggt gaacctttcg cggttggact taagtgataa 1380
ccacttctct ggtccattac cagttgaaat ttctgagatg aaaggtttgg agtttttgat 1440
tcttgcacat aaccagttta gtggcaatat accttcagaa tatggagatc ttacagcact 1500
tcaggctgtt gatctttcct ctaataggat caatggttca ataccaccaa gtttcgggaa 1560
gctaaggtca cttttgtggt tgatgcttgc aaacaattca ttgagtggtg aaatcccatc 1620
agagttgggg aactgcagca gcttgttatg gttgaatctc gcgaataatc aacttactgg 1680
tccaattcct cctcaattag cgagtattgg tgcagatcca atgctaacat ttttgttgaa 1740
taggggaaag gagaagctca ctgctagccc aggggattgt tttgccatga ggaggtggat 1800
accagctgac taccctccat ttagctttat atatcctcta ttgacaggga agagttgtag 1860
aatcctgggg gataagttgt ttatgggaga tggtttaatg ccactctgtg aacctggtag 1920
taatgtacga aagaatcagg taccgggcta tattcaactt agtgataaca aattgtctgg 1980
tgagatccct cctgagatta gcaacatgaa gaaaatgagt atgatgcatt tgggtgcaaa 2040
tgaattttct ggcaggctcc cttcagagat cgggcaactg cacctagtag tccttaatgt 2100
ttcacagaat aaattttctg gtgaaatccc aaagcagatt ggccatctta agtgcttgct 2160
aaaccttgac ctttcattta acaatttttc tggtccattc ccagttagct tcagtaactt 2220
gcatgatttg agcaagttta acatctctta caaccaatac atctatggag ctgtaccaga 2280
aaccgggcaa ctgcttactt ttgataagtc atcatttctt ggtaatccac tgttacgtct 2340
tccatccttc atgcacaact ctaagaacaa cacagaacga aacacgaatg acactcacaa 2400
aaagcccaaa aaggtgggtg cacttttggt aattgtggtt ctggtactag ccttcctagt 2460
ctgtggagtc atgtcccttg ttgtctgcct ccttataaaa gtgccaaggg gttccgcggg 2520
aatcttactg gaggatacag agggcagaca tgattctcca tcaagtacta atgcatcctc 2580
atcacggggt tctgatgatg ttaaggttat tcgtttggac agaacaagct tcacacattc 2640
tgatatactg aaggccacct ggaacttctc gaatgataga attatcggta ggggaggatt 2700
cgggatagtc tatcgtggag tcttgcctga tggaagggac gtagcagtga agaagctaca 2760
gagggaggga attgaaggag aaagagagtt cagagctgaa atggaggcgc tcagtgggaa 2820
tggctcgggt tggcctcatc ccaaccttgt aactctctat gggtggtgcc ttgatggatc 2880
agagaaactt ctagtctatg agtacatgga aggtggtacc ttagaggatt tcattacaga 2940
tcgcacaagg tttacatgga aatgccgaat tcaagcagca atcgatgtag cacgtgcttt 3000
agtctacttg caccatgatt gctacccttg cattgtccac agagatgtca aagctaacaa 3060
cgtgcttctt gacaaggacg gaagagcaaa agtcaccgat tttggccttg ccagggtcat 3120
gatttctgaa catactcatg ttagcacaat ggtggcaggg actattggtt atgttgcacc 3180
agaatatggg cagataatgc aggccactac aaaaggagat gtctacagct atggggtgct 3240
agcaatggag ctagcaactg ggaggcatgc tatagatggg ggcgaagaat gtctagttga 3300
atgggcgaca agggtcatgg gagacggaag gaaagggttc accagagcca ttataccaga 3360
tgctctattg gtacctggcc tagtagaggg agcagaggaa atgtatgagt tgcttaggat 3420
tgggataagg tgcacagctg agacgcctca tgaccggcct aacatgaagc aggtattaga 3480
tatgttgatt agtgttccta gcagccaaaa gggatccagc cgtagctttg gatcaagtcg 3540
tagcacttct ccattattat ga 3562
<210> 8
<211> 24
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 8
tgtccctatt tacgagggtt atgc 24
<210> 9
<211> 24
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 9
cagttaaatc acgaccagcc cgat 24
<210> 10
<211> 20
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 10
ctctatgggt ggtgccttga 20
<210> 11
<211> 20
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 11
ctctgtggac aatgcaaggg 20
<210> 12
<211> 28
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 12
ccctcgagca tgtctgaagt ggaatctc 28
<210> 13
<211> 28
<212> DNA
<213> 人工序列(Artificial sequence)
<400> 13
cgagctctaa atggagaagt gctacgac 28
Claims (5)
1. SlDALR1基因在增强番茄细菌性叶斑病抗性中的应用,其特征在于,所述SlDALR1基因的蛋白编码区的核苷酸序列如SEQ ID NO.1所示。
2.SlDALR1基因编码的类受体蛋白激酶在增强番茄细菌性叶斑病抗性中的应用,其特征在于,所述类受体蛋白激酶的氨基酸序列如SEQ ID NO.2所示。
3.一种培育抗细菌性叶斑病的番茄的方法,其特征在于,包括以下步骤:
(1)构建含SlDALR1基因的过表达载体;所述SlDALR1基因的蛋白编码区的核苷酸序列如SEQ ID NO.1所示;
(2)将所述过表达载体转入农杆菌感受态细胞中,构建含过表达载体的基因工程菌;
(3)将所述基因工程菌介导转化番茄子叶,培育得到转基因纯合株系。
4.如权利要求3所述的方法,其特征在于,在步骤(1)过表达载体的制备过程中,采用的上游引物如SEQ ID NO.5所示,下游引物如SEQ ID NO.6所示。
5.如权利要求3所述的方法,其特征在于,所述农杆菌为根癌农杆菌GV3101。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910085289.4A CN109734784B (zh) | 2019-01-29 | 2019-01-29 | SlDALR1基因在增强番茄细菌性叶斑病抗性中的应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910085289.4A CN109734784B (zh) | 2019-01-29 | 2019-01-29 | SlDALR1基因在增强番茄细菌性叶斑病抗性中的应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109734784A CN109734784A (zh) | 2019-05-10 |
CN109734784B true CN109734784B (zh) | 2020-06-30 |
Family
ID=66366550
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910085289.4A Active CN109734784B (zh) | 2019-01-29 | 2019-01-29 | SlDALR1基因在增强番茄细菌性叶斑病抗性中的应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109734784B (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109913466A (zh) * | 2019-03-12 | 2019-06-21 | 天津大学 | 佛甲草耐Cd基因SlSERK3及其应用 |
CN110358778B (zh) * | 2019-08-28 | 2020-09-11 | 浙江大学 | SlDALR2基因在提高番茄细菌性叶斑病抗性中的应用 |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105802977A (zh) * | 2016-04-22 | 2016-07-27 | 浙江大学 | 一种番茄ATG8f基因的克隆及其应用 |
-
2019
- 2019-01-29 CN CN201910085289.4A patent/CN109734784B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN109734784A (zh) | 2019-05-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108588087B (zh) | 一种提高植物抗病性的基因GmLecRK-R及其应用 | |
CN109777810B (zh) | Pub41基因作为负调控因子在提高番茄灰霉病和青枯病抗性中的应用 | |
CN111662914A (zh) | 水稻耐盐胁迫基因OsBAG4、编码蛋白及其应用 | |
CN114703226B (zh) | 水稻OsUBC27基因或其编码的蛋白在提高水稻产量中的应用 | |
CN109081865B (zh) | 毛竹PeVQ28蛋白及其编码基因与应用 | |
CN109734784B (zh) | SlDALR1基因在增强番茄细菌性叶斑病抗性中的应用 | |
CN110862996B (zh) | 一段分离的大豆基因在提高大豆孢囊线虫抗性中的应用 | |
CN111944829A (zh) | 一种桃叶绿体发育基因PpGLK1及其应用 | |
CN109971766A (zh) | 一种与植物耐逆相关蛋白PwRBP1及其编码基因与应用 | |
CN111778226B (zh) | 一种水稻耐碱胁迫相关的质膜H+-ATPase蛋白及其应用 | |
CN114317569A (zh) | 苹果基因MdBGLU40及在苹果树抗腐烂病中的应用 | |
CN110358778B (zh) | SlDALR2基因在提高番茄细菌性叶斑病抗性中的应用 | |
CN110885842A (zh) | 番茄TGase基因在提高番茄非生物胁迫抗性中的应用 | |
CN115161332B (zh) | 一种刺葡萄VdERF2基因及其编码蛋白和应用 | |
CN112851779B (zh) | 一种培育花青苷含量提高的转基因植物的方法 | |
CN114805520B (zh) | 胁迫抗性相关蛋白IbGT1及其编码基因及应用 | |
CN110358774A (zh) | 控制水稻开花时间的基因、蛋白质、基因表达盒、表达载体、宿主细胞、方法及应用 | |
CN109053870A (zh) | AtERF49基因在植物响应高温胁迫过程中的应用 | |
CN115011631B (zh) | 调控玉米苗期抗旱性的蛋白及其编码基因和应用 | |
CN116003563B (zh) | 钙调素结合蛋白CaMBP13在调控植物耐冷性中的应用 | |
CN115058432B (zh) | 一种烟草NtWRKY51基因及其在调控烟草青枯病抗性中的应用 | |
CN118406669B (zh) | 一种硫醇过氧化物酶编码基因及其应用 | |
CN113980986B (zh) | 一个crk22基因及其编码蛋白在马铃薯抗逆育种中的应用 | |
CN116004646B (zh) | 一种烟草NtSWEET11基因及其应用 | |
CN114292317B (zh) | 油菜基因BnCAMTA3的抗病调控功能应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |