CN109731025B - Compound skin cream for treating skin wrinkles, preparation method and application thereof - Google Patents
Compound skin cream for treating skin wrinkles, preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a compound skin cream for treating skin wrinkles, a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine external drugs. The compound skin cream is prepared from the following raw materials in parts by weight: 0.1395 parts of sheep placenta powder extract, 0.279 part of turtle shell powder extract, 0.1 part of safflower extract, 0.24 part of ginseng stem and leaf extract, and 0.2 part of oil phase, water phase and essence; the oil phase is prepared from the following raw materials in parts by weight: 10 parts of dimethyl silicone oil, 13 parts of stearic acid, 2 parts of lanolin and 2.2 parts of yellow vaseline; the water phase is prepared from the following raw materials in parts by weight: 0.1 part of ethylparaben, 3 parts of triethanolamine, 5 parts of glycerol, 0.2 part of essence and 63.7415 parts of water. The invention has the advantages that: the ointment is in a cream dosage form, is directly applied to an affected part, relieves the pain of a patient during treatment in the process of relieving skin wrinkles, and reduces wounds; has effects in scavenging free radicals, delaying aging, and eliminating wrinkle.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine external drugs, and particularly relates to a compound skin cream for treating skin wrinkles, and a preparation method and application thereof.
Background
Wrinkles refer to the formation of free radicals in the skin, which are affected by the external environment and itself, and which destroy collagen and active substances in normal cell membrane tissues. Resulting in a gradual decrease in collagen content in the dermal layer of the skin. The net support also becomes thicker and stiffer, loses elasticity, and when the elasticity and water retention of the dermis layer are reduced, the skin loses elasticity and becomes thinner and older. The epidermis forms loose wrinkles.
When various injuries cannot be repaired, the human skin shows signs of photoaging, which are mainly manifested by dry, rough, wrinkled, loose, atrophic, mottled pigmentation, etc. Generally, women begin to wrinkle at age 30-35 years old, and men begin to wrinkle at age 35-40 years old. The appearance of wrinkles obviously makes people feel that aging is reduced and brings mental stress to people. Researches find that the skin aging condition is greatly influenced by race, region, climatic environment, living habits and nursing modes, and correct skin nursing can reduce skin damage to the maximum extent and delay skin aging.
At present, a plurality of techniques and methods for removing wrinkles bring good news to beauty lovers, but at the same time, the methods for removing wrinkles are mostly invasive and bring complications such as pigmentation and the like. Once stopping taking medicine, the patient will suffer more pain.
Disclosure of Invention
The invention aims to disclose a compound skin cream for treating skin wrinkles.
The second purpose of the invention is to disclose a preparation method of the compound skin cream for treating skin wrinkles.
The third purpose of the invention is to disclose the application of the compound skin cream for treating skin wrinkles.
The purpose of the invention is realized by the following technical scheme:
a compound skin cream for treating skin wrinkles is prepared from the following raw materials in parts by weight: 0.1395 parts of sheep placenta powder extract, 0.279 part of turtle shell powder extract, 0.1 part of safflower extract, 0.24 part of ginseng stem and leaf extract, and 0.2 part of oil phase, water phase and essence;
the oil phase is prepared from the following raw materials in parts by weight: 10 parts of dimethyl silicone oil, 13 parts of stearic acid, 2 parts of lanolin and 2.2 parts of yellow vaseline;
the water phase is prepared from the following raw materials in parts by weight: 0.1 part of ethylparaben, 3 parts of triethanolamine, 5 parts of glycerol, 0.2 part of essence and 63.7415 parts of water;
the sheep placenta powder extract is prepared by the following method: extracting the sheep placenta powder with 0.3 weight part of 6.25 times of water twice, mixing the filtrates, and concentrating to 0.1395 weight parts to obtain sheep placenta powder extract;
the turtle shell powder extract is prepared by the following method: extracting carapax Trionycis powder 0.6 weight parts with 6 times of water for 2 times, mixing filtrates, and concentrating to 0.279 weight parts to obtain carapax Trionycis powder extract;
the safflower extract is prepared by the following method: soaking Carthami flos in water, decocting for 2 times, 8 times of water for 1 hr each time, filtering the extractive solution, mixing filtrates, concentrating under reduced pressure, adding 2 times of ethanol under stirring, refrigerating for 48 hr, separating supernatant, recovering ethanol, and concentrating until each weight of Carthami flos contains 1 weight of extract of the materials to obtain Carthami flos extract;
the ginseng stem and leaf extract is prepared by the following method: soaking Ginseng radix in water, decocting for 2 times, 8 times of water for 1 hr, filtering extractive solution, mixing filtrates, concentrating under reduced pressure, adding 2 times of ethanol under stirring, refrigerating for 48 hr, separating supernatant, recovering ethanol, and concentrating until the extract contains 2.4 weight parts of medicinal material extract per weight part of extract to obtain Ginseng radix stem and leaf extract.
A preparation method of compound skin cream for treating skin wrinkles comprises the following steps:
(1) and preparing a safflower extract: soaking Carthami flos in water, decocting for 2 times, 8 times of water for 1 hr each time, filtering the extractive solution, mixing filtrates, concentrating under reduced pressure, adding 2 times of ethanol under stirring, refrigerating for 48 hr, separating supernatant, recovering ethanol, and concentrating until each weight of Carthami flos contains 1 weight of extract of the materials to obtain Carthami flos extract;
preparing ginseng stem and leaf extract: soaking Ginseng radix in water, decocting for 2 times, 8 times of water for 1 hr each time, filtering the extractive solution, mixing filtrates, concentrating under reduced pressure, adding 2 times of ethanol under stirring, refrigerating for 48 hr, separating supernatant, recovering ethanol, and concentrating until the extract of Ginseng radix stem and leaf contains 2.4 weight parts of medicinal material extract per weight part of extract to obtain Ginseng radix stem and leaf extract;
(2) and preparing a sheep placenta powder extract: extracting the sheep placenta powder by 0.3 weight part and 6.25 times of water twice, combining filtrates, and concentrating to 0.1395 weight parts;
preparing a turtle shell powder extract: extracting carapax Trionycis powder 0.6 weight parts with 6 times of water for 2 times, mixing filtrates, and concentrating to 0.279 weight parts;
(3) and preparing an oil phase: weighing the following raw materials in parts by weight, 10 parts of dimethyl silicone oil, 13 parts of stearic acid, 2 parts of lanolin and 2.2 parts of yellow vaseline; (ii) a Adding simethicone, stearic acid, lanolin and yellow vaseline into an oil pan, heating to 70 ℃, and keeping the temperature for later use;
(4) and preparing an aqueous phase: weighing the following raw materials, 0.1 part of ethylparaben, 3 parts of triethanolamine, 5 parts of glycerol and 63.7415 parts of water; heating and dissolving glycerol, ethylparaben, triethanolamine and purified water to obtain water phase;
(5) and emulsification: heating the oil phase and the water phase prepared in the steps (3) and (4) and stirring to 70 ℃, firstly pumping the water phase into the main pot and sieving by a 120-mesh sieve, then pumping the oil phase into the main pot and sieving by a 120-mesh sieve, starting stirring at 35.0Hz, wherein the emulsification frequency is 50.0Hz, and the emulsification time is 20 minutes;
(6) heating the main pot to 62 ℃, adding 0.1 part by weight of the safflower extract prepared in the step (1), 0.24 part by weight of the ginseng stem and leaf extract, 0.1395 parts by weight of the sheep placenta powder extract prepared in the step (2) and 0.279 part by weight of the turtle shell powder extract, wherein the emulsifying frequency is 50.0Hz, and the emulsifying time is 20 minutes; keeping the temperature and stirring frequency at 35.0Hz for 20 minutes; transferring into a high-pressure homogenizer at the same temperature, circulating for 3 circles under the milk uniform pressure of 10000psi, taking out, introducing cooling water to reduce the temperature to below 50 ℃, and stirring at the frequency of 30.0 Hz;
(7) cooling to 55 ℃, and adding 0.2 part by weight of ginger flower essence; cooling to 37 deg.C, and discharging to obtain compound skin cream for reducing and relieving skin wrinkle.
The compound skin cream for treating the skin wrinkles is prepared by the preparation method of the technical scheme.
The application of the compound skin cream for treating the skin wrinkles in the technical scheme in the preparation of the medicine for treating the skin wrinkles.
The invention relates to a compound skin cream for treating skin wrinkles, which mainly comprises effective components such as sheep placenta, turtle shell, safflower, ginseng stem and leaf extract, and the effective components have the following functions:
the sheep placenta and the extract thereof contain rich biological active substances such as micromolecular polypeptide, enzyme, amino acid, nucleic acid and the like, and have certain effects on improving the oxidation resistance and the anti-aging capability of an organism, enhancing the immunity, protecting and nourishing the skin and regulating the sleep;
carapax Trionycis has effects of nourishing yin, eliminating hyperactivity of yang, removing heat, removing toxic substance, softening and resolving hard mass, and is commonly used for treating fever due to yin deficiency, bone steaming, fatigue, yin deficiency, hyperactivity of yang, dizziness, blurred vision, deficient wind, convulsion of limbs, amenorrhea, abdominal mass, and permanent malaria. Carapax Trionycis has pharmacological activities of relieving fatigue, regulating immunity and protecting liver, and contains animal glue, keratin, iodine, vitamin D, amino acids, etc.;
safflower is pungent and warm in nature and flavor, and has the main functions of activating blood and dredging collaterals, and removing blood stasis and relieving pain, and enters heart and liver meridians. The chemical components of safflower are complex, and are divided into more than two hundred compounds such as flavonoids, polyacetylenes, indoles, steroids, lignins, fatty acids, volatile oil, alkyl glycols, organic acids, sterols and the like. Carthami flos has pharmacological activities of dilating blood vessel, increasing blood flow, improving microcirculation, inhibiting platelet aggregation, exciting uterus, lowering blood pressure, resisting cancer, and resisting inflammation;
ginseng is neutral in nature, sweet, slightly bitter and slightly warm in taste. The ginseng has the effects of tonifying primordial qi, recovering pulse, relieving depletion, tonifying spleen, benefiting lung, promoting production of body fluid, quenching thirst, calming nerves and benefiting intelligence, is known as a key medicine for tonifying primordial qi, contains more than 40 Ginsenoside (GS) as a main component, Ginseng Polysaccharide (GPS), panaxynol, volatile oil, low molecular peptide, amino acid, vitamin, organic acid and multiple trace elements, can nourish and strengthen the body, and has curative effects in preventing tumor, resisting aging, resisting arrhythmia, inhibiting apoptosis, reducing blood sugar and blood fat, improving learning and memory, enhancing sexual function and immunologic function, relieving alcoholism and the like;
the four components cooperate with each other, integrate anti-aging, blood circulation promotion and beauty and skin care into a whole, and achieve good treatment effect on the aspect of reducing and relieving skin wrinkles.
The invention has the following beneficial effects:
1. the invention is in a cream dosage form, is directly applied to an affected part, relieves the pain of a patient during treatment and reduces the wound in the process of relieving skin wrinkles.
2. The invention adopts a formula of combining four traditional Chinese medicines, and mainly can resist free radicals, delay senility and remove wrinkles.
The specific implementation mode is as follows:
in order to facilitate understanding of the technical scheme of the invention, the compound skin cream (hereinafter, compound sheep placenta cream) for treating skin wrinkles, the preparation method and the application thereof are further described by combining specific embodiments.
Example 1: a compound skin cream for treating skin wrinkles comprises:
(1) and preparing a safflower extract: soaking Carthami flos in water, decocting for 2 times, 8 times of water for 1 hr, filtering extractive solution, mixing filtrates, concentrating under reduced pressure, adding 2 times of ethanol under stirring, refrigerating for 48 hr, separating supernatant, recovering ethanol, and concentrating until Carthami flos extract contains 1g extract of Carthami flos per g extract to obtain Carthami flos extract;
preparing ginseng stem and leaf extract: soaking Ginseng radix in water, decocting for 2 times, 8 times of water for 1 hr, filtering extractive solution, mixing filtrates, concentrating under reduced pressure, adding 2 times of ethanol under stirring, refrigerating for 48 hr, separating supernatant, recovering ethanol, and concentrating until the extract of Ginseng radix stem and leaf contains 2.4g medicinal material extract per g extract to obtain Ginseng radix stem and leaf extract;
(2) and preparing a sheep placenta powder extract: extracting sheep placenta powder 3g and water 6.25 times the weight of the sheep placenta powder twice, combining filtrates, and concentrating to 1.395 g;
preparing a turtle shell powder extract: extracting carapax Trionycis powder 6g with 6 times of water for 2 times, mixing filtrates, and concentrating to 2.79 g;
(3) and preparing an oil phase: weighing 100g of dimethyl silicone oil, 130g of stearic acid, 20g of lanolin and 22g of yellow vaseline; adding simethicone, stearic acid, lanolin and yellow vaseline into an oil pan, heating to 70 ℃, and keeping the temperature for later use;
(4) and preparing an aqueous phase: weighing 1g of ethylparaben, 30g of triethanolamine, 50g of glycerol and 637.415g of water; heating and dissolving glycerol, ethylparaben, triethanolamine and purified water to obtain water phase;
(5) and emulsification: heating the oil phase and the water phase prepared in the steps (3) and (4) and stirring to 70 ℃, firstly pumping the water phase into the main pot and sieving by a 120-mesh sieve, then pumping the oil phase into the main pot and sieving by a 120-mesh sieve, starting stirring at 35.0Hz, wherein the emulsification frequency is 50.0Hz, and the emulsification time is 20 minutes;
(6) heating the main pot to 62 ℃, adding 1g of safflower extract prepared in the step (1), 2.4g of ginseng stem and leaf extract, 1.395g of sheep placenta powder extract prepared in the step (2) and 2.79g of turtle shell powder extract, wherein the emulsifying frequency is 50.0Hz, and the emulsifying time is 20 minutes; keeping the temperature and stirring frequency at 35.0Hz for 20 minutes; transferring into a high-pressure homogenizer at the same temperature, circulating for 3 circles under the milk uniform pressure of 10000psi, taking out, introducing cooling water to reduce the temperature to below 50 ℃, and stirring at the frequency of 30.0 Hz;
(7) cooling to 55 ℃, and adding 2g of ginger flower essence; cooling to 37 deg.C, and discharging to obtain compound skin cream for reducing and relieving skin wrinkle.
The beneficial effects of the compound skin cream (hereinafter, compound sheep placenta cream) for treating skin wrinkles are described in the following by specific test examples.
Test example 1: the extraction solution process flow and the cream matrix screening:
firstly, turtle shell extraction process:
in the recipe, Bie Jia is the drug of hornet class, and the decoction is usually decocted first. The research separately extracts the turtle shell, takes nitrogen content as an examination index, and preliminarily discusses the crushing degree, water adding amount, extraction time, extraction times and the like of the turtle shell by an orthogonal test method.
The decoction equivalent to about 1.0 g of the medicinal materials is precisely weighed, and the nitrogen content is determined according to a nitrogen determination method of four parts 0704 in 2015 edition of Chinese pharmacopoeia.
Influence of turtle shell medicinal material particle size on nitrogen extraction amount 6 parts of turtle shell are taken, crushed to different particle sizes respectively, then 10.0g of turtle shell is weighed respectively, added with 8 times of water and decocted for 1 time, 1 hour each time, and the content of extracted liquid nitrogen is measured, and the results are shown in table 1.
TABLE 1 determination of nitrogen content in turtle shell decoction with different particle sizes
As is clear from the results in Table 1, the higher the pulverizing degree of carapax Trionycis, the higher the nitrogen content in the extract. However, the smaller the pulverized particle size, the difficulty of filtration is, which is not favorable for mass production. Thus, the turtle shell was pulverized to coarse particles, 10 mesh, 24 mesh coarse powder 3 levels, and orthogonal experiments were performed.
TABLE 2 turtle shell water extraction process orthogonal design factor horizon
The same lot of samples, each 20.0g, was weighed into 9 groups and extracted as shown in Table 2. Filtering the extractive solution while it is hot, mixing filtrates, cooling, precisely weighing extractive solution corresponding to 1g, measuring nitrogen content, and repeating for 3 times. The experimental results and the analysis of variance are shown in tables 3 and 4.
TABLE 3L 9 (3)4) Orthogonal test table and results
TABLE 4 ANOVA TABLE
The analysis of variance result shows that A, D factor has significant influence, and the optimal scheme is A3B2C2D2Since B has no significant difference, A is selected in view of energy saving and cost reduction3B1C2D2The turtle shell is sieved by a 24-mesh sieve, 6 times of water is added, and the extraction is carried out for 2 times after 1 hour of each extraction.
Secondly, the extraction process of the ginseng medicinal material comprises the following steps:
1. index measurement:
(1) chromatographic conditions for HPLC measurement of ginseng: chromatographic column (C)18) Acetonitrile is taken as a mobile phase A, water is taken as a mobile phase B, and gradient elution is carried out according to the specification in the following table; the detection wavelength was 203 nm. The theoretical plate number is determined according to ginsenoside Rg1The peak should be no less than 6000.
(2) And preparing a reference substance solution: precisely weighing ginsenoside Rg1For the control, methanol was added to make a solution containing 0.2mg per 1 ml.
(3) And preparing a test solution: taking about 1g of the product powder (passing through a No. four sieve), precisely weighing, placing in a Soxhlet extractor, adding chloroform, heating and refluxing for 3 hours, discarding chloroform liquid, volatilizing solvent from medicine residues, transferring into a 100ml conical flask together with a filter paper cylinder, precisely adding 50ml of water-saturated n-butanol, sealing, standing overnight, performing ultrasonic treatment (power 250w and frequency 50 kHz) for 30 minutes, filtering, discarding primary filtrate, precisely weighing 25ml of secondary filtrate, placing in an evaporating dish, evaporating to dryness, dissolving residues with methanol, transferring into a 5ml measuring flask, adding methanol to dilute to scale, shaking uniformly, filtering, and taking secondary filtrate to obtain the product.
2. Determination of cream yield:
taking 100g of ginseng stem leaf extract, placing the ginseng stem leaf extract in an evaporating dish which is dried to constant weight, evaporating the ginseng stem leaf extract in a water bath, and drying the ginseng stem leaf extract to constant weight at 105 ℃.
3. Study on water extraction of Ginseng radix
Taking the medicinal materials according to the proportion of the prescription, determining the final extraction process by using a 3-factor 3 horizontal orthogonal design table with water addition, extraction time and extraction times as factors, and showing in a table 5; the results are shown in Table 6 and the analysis of variance is shown in Table 7.
TABLE 5 level table of orthogonal design factors for extraction process of ginseng
TABLE 6L 9 (3)4) Orthogonal test table and results
TABLE 7 ANOVA TABLE
The orthogonal test result and the variance analysis show that the magnitude sequence of the factors influencing the transfer rate% and the cream yield of the ginsenoside Rg1 is C>A>B, wherein the factors A and C are related to ginsenoside Rg1The transfer rate of the transfer rate% has a significant influence, and the factor C has a significant influence on the cream yield. The optimal extraction process is determined to be A by combining the production cost and energy conservation and comprehensively considering2B1C2. I.e. 8 times of water, 1h each time, 2 times of extraction.
Thirdly, screening the cream matrix:
the cream base of the invention is screened on the basis of referring to other formed cream bases.
The preparation method comprises the following steps: weighing oil phase components according to the formula, placing the water phase components in different beakers, heating and melting the water phase components in water bath to 80 ℃, gradually adding the oil phase into the water phase (80 ℃), stirring at a proper stirring speed, emulsifying (80 ℃ water bath heat preservation) for 30 minutes, stopping the water bath, continuously stirring and cooling to room temperature, taking a proper amount of cream matrix, mixing the cream matrix with the liquid medicine, and observing the state of the cream.
Run one (water phase poured into oil phase):
oil phase: liquid Paraffin 6g
Vaseline 5g
Cetyl alcohol 12g
Lanolin 3g
Water phase: sodium dodecyl sulfate 2g
Peregal 1.5g
Glycerol 5g
Purified water to 100g
Run two (oil phase poured into water phase):
oil phase: liquid Paraffin 6g
Vaseline 5g
Cetyl alcohol 12g
Lanolin 3g
Water phase: sodium dodecyl sulfate 2g
Peregal 1.5g
Glycerol 5g
Purified water to 100g
And (3) test III:
oil phase: liquid Paraffin 6g
Vaseline 5g
Cetyl alcohol 6g
Octadecanol 6g
Lanolin 3g
Water phase: sodium dodecyl sulfate 2g
Peregal 1.5g
Glycerol 8g
Purified water to 100g
And (4) testing:
oil phase: cetyl alcohol: 6g
Octadecanol: 6g
Liquid Paraffin 6g
Vaseline 5g
Lanolin 3g
Water phase: sodium dodecyl sulfate 2g
Peregal 2g
Glycerol 10g
Purified Water 100g Total 140g
And (5) testing:
oil phase: liquid Paraffin 3g
Vaseline 2.5g
Cetyl alcohol 3g
Octadecanol 3g
Lanolin 1.5g
Water phase: sodium dodecyl sulfate 2g
Peregal 2g
Glycerol 10g
120g of purified water amounting to 150g
And (6) test six:
oil phase: stearic acid 4.8g
Glyceryl monostearate 1.4g
Liquid paraffin 2.4g
White Vaseline 0.4g
Lanolin 2g
Water phase: triethanolamine 0.16g
Purified water to 40g
Test seven:
oil phase: stearic acid 1.4g
Glyceryl monostearate 0.7g
Liquid paraffin 1.2
White Vaseline 0.2g
Lanolin 1g
Water phase: glycerol 4.42g
Triethanolamine 0.08g
Purified water to 60g
And (eight) test:
oil phase: liquid Paraffin 2g
Vaseline 1.3g
Cetyl alcohol 2g
Octadecanol 2g
Lanolin 0.7g
Water phase: sodium dodecyl sulfate 1g
Peregal 1g
Glycerol 11g
Nipagin ethyl ester 0.25g
Adding purified water to 100g
Test nine:
oil phase: dimethicone 10g
Stearic acid 13g
Yellow Vaseline 2.2g
Lanolin 2g
Water phase: 0.1g of ethylparaben
Triethanolamine 3g
Glycerol 5g
Adding purified water to 100g
TABLE 8 results of matrix tests
Through the tests, the cream matrix of the compound sheep placenta milk is preliminarily prepared
Oil phase: dimethicone 10g
Stearic acid 13g
Yellow Vaseline 2.2g
Lanolin 2g
Water phase: 0.1g of ethylparaben
Triethanolamine 3g
Glycerol 5g
Adding purified water to 100g
According to the process requirements of the compound sheep placenta essence milk, the ginger flower essence is selected as a flavoring agent, so that the odor of the ointment is improved, and the use acceptance of a patient is improved.
TABLE 9 proportions of extract, matrix and flavor
According to the test result, the prescription proportion is adjusted, and the preliminary tentative prescription is as follows:
turtle shell powder extract 2.79g
Sheep placenta powder extract 1.395g
Safflower extract 1g
Ginseng Stem and leaf extract 2.4g
Dimethicone 100g
Stearic acid 130g
Lanolin 20g
Yellow Vaseline 22g
Ethylparaben 1g
Triethanolamine 30g
Glycerol 50g
Essence 2g
Water 637.415g
Making into 1000g
TABLE 10 results of the three-batch Compound sheep placenta essence milk cream shaping process
The results of the reproducibility test show that the three batches of sample creams are uniform, have moderate color and appropriate fragrance, and meet the regulations.
Test example 2: animal experiment of compound sheep placenta emulsion for treating skin wrinkles:
1. the material and the method are as follows:
1.1 reagent:
d-galactose (supplied by Shanghai gathering biological laboratory); compound sheep placenta milk (prepared in example 1); physiological saline (Sichuan Kelun pharmaceutical Co., Ltd.); other reagents are all domestic analytical purifiers.
1.2 animals:
healthy 1-month-old male nude mice 30 (IVC fed), body mass (14g) (provided by Experimental animals center of military medical academy of sciences),
1.3 Instrument:
a cryogenic refrigerator (NU-6352E); ultraviolet spectrophotometer (model DU-800); an electronic balance (BP110 type), an ultraviolet intensity meter (KUV 254 type); 2 long and short wavelength uv lamps each.
1.4 animal grouping, dosing and manufacturing model:
each group was 10, randomly divided into source groups:
1. normal group: feeding under normal condition without injection and illumination treatment;
2. model group: injecting D-galactose (1000mg/kg) into neck and back of the patient daily, and irradiating back skin with long wavelength (365nm) and short wavelength (254nm) ultraviolet rays, wherein the cumulative irradiation intensity of the long wavelength ultraviolet rays is 259.5/cm2Cumulative short wavelength radiation intensity 6.482J/cm2；
3. Compound sheep placenta extract milk group: injecting and illuminating with the same model group, and smearing compound foetus Caprae Seu Ovis essence milk on the back of the injection and illumination group, 2 times daily, 0.2 g/time.
The test results are shown in Table 11
TABLE 11 different sets of wrinkle resistance results
II, experimental results:
after continuous injection and illumination for 6 weeks, the model group showed obvious wrinkles and obvious aging signs after bradykinesia. The wrinkles on the back of the mice coated with the compound sheep placenta milk are obviously reduced.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims; meanwhile, any equivalent changes, modifications and variations of the above embodiments according to the essential technology of the present invention are within the scope of the technical solution of the present invention.
Claims (4)
1. A compound skin cream for treating skin wrinkles is characterized in that: the compound skin cream is prepared from the following raw materials in parts by weight: 0.1395 parts of sheep placenta powder extract, 0.279 part of turtle shell powder extract, 0.1 part of safflower extract, 0.24 part of ginseng stem and leaf extract, and 0.2 part of oil phase, water phase and essence;
the oil phase is prepared from the following raw materials in parts by weight: 10 parts of dimethyl silicone oil, 13 parts of stearic acid, 2 parts of lanolin and 2.2 parts of yellow vaseline;
the water phase is prepared from the following raw materials in parts by weight: 0.1 part of ethylparaben, 3 parts of triethanolamine, 5 parts of glycerol, 0.2 part of essence and 63.7415 parts of water;
the sheep placenta powder extract is prepared by the following method: extracting the sheep placenta powder with 0.3 weight part of 6.25 times of water twice, mixing the filtrates, and concentrating to 0.1395 weight parts to obtain sheep placenta powder extract;
the turtle shell powder extract is prepared by the following method: extracting carapax Trionycis powder 0.6 weight parts with 6 times of water for 2 times, mixing filtrates, and concentrating to 0.279 weight parts to obtain carapax Trionycis powder extract;
the safflower extract is prepared by the following method: soaking Carthami flos in water, decocting for 2 times, 8 times of water for 1 hr each time, filtering the extractive solution, mixing filtrates, concentrating under reduced pressure, adding 2 times of ethanol under stirring, refrigerating for 48 hr, separating supernatant, recovering ethanol, and concentrating until each weight of Carthami flos contains 1 weight of extract of the materials to obtain Carthami flos extract;
the ginseng stem and leaf extract is prepared by the following method: soaking Ginseng radix in water, decocting for 2 times, 8 times of water for 1 hr, filtering extractive solution, mixing filtrates, concentrating under reduced pressure, adding 2 times of ethanol under stirring, refrigerating for 48 hr, separating supernatant, recovering ethanol, and concentrating until the extract contains 2.4 weight parts of medicinal material extract per weight part of extract to obtain Ginseng radix stem and leaf extract.
2. A preparation method of compound skin cream for treating skin wrinkles comprises the following steps:
(1) and preparing a safflower extract: soaking Carthami flos in water, decocting for 2 times, 8 times of water for 1 hr each time, filtering the extractive solution, mixing filtrates, concentrating under reduced pressure, adding 2 times of ethanol under stirring, refrigerating for 48 hr, separating supernatant, recovering ethanol, and concentrating until each weight of Carthami flos contains 1 weight of extract of the materials to obtain Carthami flos extract;
preparing ginseng stem and leaf extract: soaking Ginseng radix in water, decocting for 2 times, 8 times of water for 1 hr each time, filtering the extractive solution, mixing filtrates, concentrating under reduced pressure, adding 2 times of ethanol under stirring, refrigerating for 48 hr, separating supernatant, recovering ethanol, and concentrating until the extract of Ginseng radix stem and leaf contains 2.4 weight parts of medicinal material extract per weight part of extract to obtain Ginseng radix stem and leaf extract;
(2) and preparing a sheep placenta powder extract: extracting the sheep placenta powder by 0.3 weight part and 6.25 times of water twice, combining filtrates, and concentrating to 0.1395 weight parts;
preparing a turtle shell powder extract: extracting carapax Trionycis powder 0.6 weight parts with 6 times of water for 2 times, mixing filtrates, and concentrating to 0.279 weight parts;
(3) and preparing an oil phase: weighing the following raw materials in parts by weight, 10 parts of dimethyl silicone oil, 13 parts of stearic acid, 2 parts of lanolin and 2.2 parts of yellow vaseline; (ii) a Adding simethicone, stearic acid, lanolin and yellow vaseline into an oil pan, heating to 70 ℃, and keeping the temperature for later use;
(4) and preparing an aqueous phase: weighing the following raw materials, 0.1 part of ethylparaben, 3 parts of triethanolamine, 5 parts of glycerol and 63.7415 parts of water; heating and dissolving glycerol, ethylparaben, triethanolamine and purified water to obtain water phase;
(5) and emulsification: heating the oil phase and the water phase prepared in the steps (3) and (4) and stirring to 70 ℃, firstly pumping the water phase into the main pot and sieving by a 120-mesh sieve, then pumping the oil phase into the main pot and sieving by a 120-mesh sieve, starting stirring at 35.0Hz, wherein the emulsification frequency is 50.0Hz, and the emulsification time is 20 minutes;
(6) heating the main pot to 62 ℃, adding 0.1 part by weight of the safflower extract prepared in the step (1), 0.24 part by weight of the ginseng stem and leaf extract, 0.1395 parts by weight of the sheep placenta powder extract prepared in the step (2) and 0.279 part by weight of the turtle shell powder extract, wherein the emulsifying frequency is 50.0Hz, and the emulsifying time is 20 minutes; keeping the temperature and stirring frequency at 35.0Hz for 20 minutes; transferring into a high-pressure homogenizer at the same temperature, circulating for 3 circles under the milk uniform pressure of 10000psi, taking out, introducing cooling water to reduce the temperature to below 50 ℃, and stirring at the frequency of 30.0 Hz;
(7) cooling to 55 ℃, and adding 0.2 part by weight of ginger flower essence; cooling to 37 deg.C, and discharging to obtain compound skin cream for reducing and relieving skin wrinkle.
3. A compound skin cream for treating skin wrinkles is characterized in that: the compound skin cream is prepared by the preparation method of claim 2.
4. Use of the compound skin cream for treating skin wrinkles of claim 1 or 3 in the preparation of a medicament for treating skin wrinkles.
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| CN101422422A (en) * | 2008-12-19 | 2009-05-06 | 美晨集团股份有限公司 | Sheep placental extract paste and manufacture method thereof |
| CN103385843A (en) * | 2013-07-22 | 2013-11-13 | 吴万有 | Speckle-removing composition, preparation method thereof and applications thereof |
| CN107823260A (en) * | 2017-11-20 | 2018-03-23 | 蔡瑞康 | A kind of compound skin cream for treating chloasma, preparation method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101422422A (en) * | 2008-12-19 | 2009-05-06 | 美晨集团股份有限公司 | Sheep placental extract paste and manufacture method thereof |
| CN103385843A (en) * | 2013-07-22 | 2013-11-13 | 吴万有 | Speckle-removing composition, preparation method thereof and applications thereof |
| CN107823260A (en) * | 2017-11-20 | 2018-03-23 | 蔡瑞康 | A kind of compound skin cream for treating chloasma, preparation method and application |
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| 跨世纪美容极品 羊胎素;张瑾;《城市质量监督》;19990115(第01期);第42页 * |
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