CN109730800B - Slow injection device that fixes a position of trace for rat brain experiments - Google Patents
Slow injection device that fixes a position of trace for rat brain experiments Download PDFInfo
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Abstract
The invention discloses a needle core pushing mechanism, which comprises a bracket, a needle pushing mechanism and a needle core pushing mechanism; the support is composed of a bottom plate, a telescopic supporting column and a top plate, a fixed table is further arranged on the bottom plate in a sliding mode, and a clamping mechanism is arranged on the fixed table in a sliding mode; the needle pushing mechanism comprises a screw fixedly arranged below the top plate, two screw sleeves symmetrically arranged on the screw, connecting rods hinged to the screw sleeves, a first connecting plate hinged to the two connecting rods together, and a fixing mechanism arranged in the middle of the first connecting plate and used for fixing the syringe, wherein the screw is composed of two sections, the two sections are integrally formed, but the spiral directions of threads on the two sections are opposite; the stylet pushing mechanism comprises a boosting cylinder, a toothed plate and a gear, wherein the boosting cylinder penetrates through the top plate and is in sliding connection with the top plate, the toothed plate is fixedly arranged at the top of the boosting cylinder, the gear is meshed with the toothed plate and can be self-locked, the gear is arranged on the top plate in a sliding mode, and a first handle and scale marks are arranged on the gear. The invention effectively avoids the error caused by long-time bare-handed fixation of the experimenter, and has reasonable structure and convenient operation.
Description
Technical Field
The invention relates to a device for rat brain experiments, in particular to a trace slow positioning injection device for rat brain experiments.
Background
Senile dementia, also known as alzheimer disease, is a primary degenerative encephalopathy occurring in the elderly and pre-senile stages, and refers to a persistent higher nerve functional activity disorder, i.e., disorders in memory, thinking, analysis and judgment, visual space recognition, emotion and the like without disturbance of consciousness; including vascular dementia and dementia with degeneration of brain nerve cells. In the research work of the senile dementia, an animal senile dementia model similar to the senile dementia of a human is often required to be established, so that medical research on the aspects of the pathological mechanism, treatment and the like of the senile dementia is carried out. For example, a rat model of senile dementia is established to study the pathogenesis of senile dementia, including a model of dementia due to cholinergic injury, a model of dementia due to aging, a model of dementia due to ABETA, a model of dementia due to chronic cerebral ischemia, and other models; the cholinergic injury dementia model comprises fornix-hippocampal ampus cutting dementia model rat, basilar forebrain injection Artemisia amansi dementia model rat and scopolamine cholinergic injury dementia-simulating mouse; the model of aging-induced dementia comprises a natural aging animal, a rapid aging mouse and a brain aging mouse model caused by subcutaneous injection of D-galactose; the ABETA dementia-causing model comprises an APP transgenic mouse and an Abeta injection dementia-causing model; other models include aluminum models, dementia due to mitochondrial defects, dementia due to chronic damage of protein phosphorylase inhibitors, natural immune dementia models, memory impairment models, in vitro screening models, electrolytic injury models, AD-mimicking models due to lipopolysaccharide, and AD models due to metabolic disorders.
In the process of establishing the senile dementia rat model, most of the models need to be constructed by injecting a reagent into the rat brain, however, in the process of constructing the rat model, because the rat brain is very small, in addition, in order to avoid experiment failure caused by the increase of the intracranial pressure of the rat, the injection reagent needs to be injected at an extremely slow speed. Meanwhile, the syringe cannot move in the whole injection process, and the needle must be retained after the injection is finished, and the needle is slowly withdrawn after the liquid is diffused into the tissue, so as to prevent the liquid from overflowing along with the injection needle head. However, such injection is performed while maintaining a posture for a long time, and the operation by hand is difficult. Therefore, it is necessary to design a micro slow positioning injection device for rat brain experiments.
Disclosure of Invention
In order to solve the defects in the prior art, the invention provides the micro slow positioning injection device for the rat brain experiment, which is simple to operate, good in fixation and injection effects and convenient to use.
The invention provides a micro slow positioning injection device for a rat brain experiment, which comprises a bracket, a needle pushing mechanism and a needle core pushing mechanism;
the support comprises a bottom plate, four telescopic support columns uniformly arranged at the top of the bottom plate and a top plate jointly connected with the four telescopic support columns, a fixed table is also arranged on the bottom plate in a sliding mode, and a clamping mechanism is arranged on the fixed table in a sliding mode;
the needle pushing mechanism comprises a screw fixedly arranged below the top plate, two screw sleeves symmetrically arranged on the screw, connecting rods hinged to the screw sleeves, a first connecting plate hinged to the two connecting rods together, and a fixing mechanism arranged in the middle of the first connecting plate and used for fixing the syringe, wherein the screw is composed of two sections, the two sections are integrally formed, but the spiral directions of threads on the two sections are opposite;
the stylet pushing mechanism comprises a boosting cylinder, a toothed plate and a gear, wherein the boosting cylinder penetrates through the top plate and is in sliding connection with the top plate, the toothed plate is fixedly arranged at the top of the boosting cylinder, the gear is meshed with the toothed plate and can be self-locked, the gear is arranged on the top plate in a sliding mode, and a first handle and scale marks are arranged on the gear.
Preferably, the fixed station comprises a fixed plate and a first slide block positioned at the bottom of the fixed plate, a first slide way capable of being connected with the first slide block in a sliding manner is arranged on the bottom plate, and a fixing bolt is further arranged on the first slide block.
Preferably, the clamping mechanism is two clamping plates which are arranged on the fixing plate in a sliding mode.
Preferably, the needle pushing mechanism further comprises two second connecting plates, the two second connecting plates are respectively arranged on two sides of the support and are fixedly connected with the top plate and the bottom plate, and the second connecting plates are further connected with the first connecting plates in a sliding mode.
Preferably, the opposite side surfaces of the two second connecting plates are correspondingly provided with second slide ways in the vertical direction, the two sides of the first connecting plate are respectively provided with a second slide block which can be in sliding connection with the second slide ways, and the second connecting plate is provided with scale marks.
Preferably, the screw rod passes through the connecting block to be connected with the roof, and screw rod one end is provided with the second handle, and the screw rod passes through the bearing and installs between two second connecting plates.
Preferably, fixed establishment is two sets of third slides, the third slide includes vertical slide and horizontal slide, first circular through-hole has been seted up at first connecting plate middle part, vertical slide sets up on the relative lateral wall in first circular through-hole, and extend to the middle part from upper portion, horizontal slide begins in the bottom of vertical slide and along the axial extension of first circular through-hole, the other end below intercommunication of horizontal slide is provided with square clamping groove, correspond on the syringe cylinder be provided with third slide sliding connection and can be fixed in the third slider in the square clamping groove.
Preferably, a second circular through hole capable of accommodating the boosting cylinder is formed in the middle of the top plate, vertical fourth slides with convex-shaped cross sections are arranged on two opposite sides in the second circular through hole, and fourth sliders capable of being in sliding connection with the fourth slides are correspondingly arranged on two sides of the boosting cylinder.
Preferably, a fifth slide way is arranged on the top plate, a fifth slide block is arranged on the fifth slide way in a sliding mode, the fifth slide block is connected with a disc damping rotating shaft through a support, and a gear is connected to the disc damping rotating shaft.
Compared with the prior art, the invention has the following beneficial effects:
the experimental rat is fixed through the fixing table and the clamping mechanism, the injector is pushed to the position for injecting the medicine and fixed through the needle pushing mechanism, and the injector is pushed through the boosting cylinder, so that the medicine enters the brain of the rat; the needle core pushing mechanism is reasonable in structure and convenient to use, the fixing table, the needle pushing mechanism and the needle core pushing mechanism can move and be fixed when needed through the support, and the influence on an experimental result caused by long-time bare-handed fixing in the experimental process is avoided; fix the syringe cylinder through fixed establishment, thereby the process of influence experiment is promoted the syringe cylinder once more when having avoided boosting section of thick bamboo effort great, realize the auto-lock of gear through disc damping pivot, avoided boosting section of thick bamboo to promote the process that the syringe mistake was injected to the rat because self action of gravity slides down, and the realization is pushed promptly and is stopped promptly, convenient operation all is provided with the scale mark on second connecting plate and the gear simultaneously, has guaranteed the accuracy of injection process.
Drawings
FIG. 1 is a schematic structural diagram of a micro slow positioning injection device for rat brain experiments according to the present invention;
fig. 2 is an enlarged view of the structure of the fixing mechanism in the micro slow positioning injection device for rat brain experiments.
In the figure: 1. a base plate; 2. a telescopic support column; 3. a top plate; 4. a fixed table; 5. a screw; 6. a threaded sleeve; 7. a connecting rod; 8. a first connecting plate; 9. a boosting cylinder; 10. a toothed plate; 11. a gear; 12. a second connecting plate; 13. a vertical slideway; 14. a horizontal slideway; 15. a first circular through hole; 16. a square clamping groove.
Detailed Description
The following are specific embodiments of the present invention and are further described with reference to the drawings, but the present invention is not limited to these embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example 1
A micro slow positioning injection device for rat brain experiments comprises a support, a needle pushing mechanism and a needle core pushing mechanism, wherein the support is used for bearing the needle pushing mechanism and the needle core pushing mechanism and fixing an experimental rat;
the support comprises a bottom plate 1, four telescopic support columns 2 uniformly arranged at the top of the bottom plate 1 and a top plate 3 connected with the four telescopic support columns 2 together, namely the telescopic support columns 2 are arranged around the top of the bottom plate 1, and the top of the four telescopic support columns 2 are connected with the top plate 3 together, wherein the telescopic support columns 2 are of an electric telescopic structure, a hydraulic telescopic structure and a mechanical telescopic structure, the telescopic support columns 2 of the electric telescopic structure are HTW1000 in model, the telescopic support columns 2 of the hydraulic telescopic structure are KLT-QTH in model, and the mechanical telescopic structure comprises an inner rod, an outer tube sleeved on the inner rod and a fixing bolt used for fixing the inner rod and the outer tube; the bottom plate 1 is also provided with a fixed table 4 for placing an experimental rat in a sliding manner, the fixed table 4 is provided with a clamping mechanism for fixing the experimental rat in a sliding manner, in the experimental process, the experimental rat is firstly placed on the fixed table 4 and is fixed by the clamping mechanism, and then the fixed table 4 is moved to the position for carrying out the experiment;
the needle pushing mechanism comprises a screw rod 5 fixedly arranged below the top plate 3, two screw sleeves 6 symmetrically arranged on the screw rod 5, a connecting rod 7 hinged on the screw sleeve 6, a first connecting plate 8 hinged with the two connecting rods 7 together and a fixing mechanism arranged in the middle of the first connecting plate 8 and used for fixing the syringe needle cylinder, wherein the screw rod 5 consists of two sections, the two sections are integrally formed, but the spiral directions of the threads on the two sections are opposite, namely the syringe needle cylinder is fixed on the first connecting plate 8, and the two screw sleeves 6 distributed on the screw rod 5 move close to each other or move away from each other by rotating the screw rod 5, so that the first connecting plate 8 moves downwards or upwards, and finally the position of the syringe needle cylinder is adjusted;
the needle core pushing mechanism comprises a boosting cylinder 9 penetrating through the top plate 3 and connected in a sliding manner, a toothed plate 10 fixedly arranged at the top of the boosting cylinder 9 and a gear 11 which is meshed with the toothed plate 10 and can be self-locked, the gear 11 is arranged on the top plate 3 in a sliding manner, a first handle and scale marks are arranged on the gear 11, the boosting cylinder 9 is used for pushing a piston of the injector to inject liquid medicine into the brain of an experimental rat after a syringe needle cylinder is inserted into the brain of the experimental rat, the boosting cylinder 9 moves downwards due to the cooperation of the toothed plate 10 and the gear 11, the gear 11 which can be self-locked effectively avoids the situation that the boosting cylinder mistakenly pushes the piston of the injector downwards under the action of self gravity, the instant stop can be realized and the operation is convenient, meanwhile, the gear 11 is provided with the scale marks which can be marked as the descending height of the boosting cylinder 9, namely, the gear 11 is rotated every time when the gear 11 rotates, the boosting cylinder can descend by a specific size, so that an operator can conveniently master the injection speed.
Example 2
The micro slow positioning injection device for the rat brain experiment is further optimized in the embodiment, based on the device in embodiment 1, the fixed table 4 is limited, the fixed table 4 comprises a fixed plate and a first sliding block positioned at the bottom of the fixed plate, a first slide way capable of being in sliding connection with the first sliding block is arranged on the bottom plate 1, and a fixing bolt is further arranged on the first sliding block; in order to strengthen the fixing effect on the experimental rat, two clamping plates which are arranged on the fixed plate in a sliding manner are set as clamping mechanisms, namely a sixth slide way is arranged on the fixed plate, a sixth slide block is connected on the sixth slide way in a sliding manner, the sixth slide block is connected with a clamping plate, and a fixing bolt is also arranged on the sixth slide block;
when needs are fixed to experimental rat, at first place experimental rat in the suitable position of fixed plate, then through slide and fixed sixth slider, use the grip block to fix experimental rat, rethread slip and fixed first slider after that, it is fixed with fixed station 4, make experimental rat's brain just in time be located the below of syringe.
Example 3
This embodiment is based on embodiment 1's device, optimize the sliding connection relation of roof 3 and boosting section of thick bamboo 9 of the device, set up in the middle part of roof 3 and can hold the circular through-hole of second of boosting section of thick bamboo 9, relative both sides are provided with the vertical fourth slide that the cross section is type-protruding in the circular through-hole of second, boosting section of thick bamboo 9 both sides correspondence be provided with can with fourth slide sliding connection's fourth slider, the cross section of fourth slide is type-protruding, the fourth slider is the arch that matches with the fourth slide, through the setting of type-protruding of fourth slide and fourth slider, can avoid boosting section of thick bamboo to take place rotatoryly by the promotion in-process to gear 11 idle running has been avoided.
Example 4
In this embodiment, based on the device of embodiment 1, the self-locking function of the gear 11 in the device is optimized, specifically, a fifth slide is arranged on the top plate 3, a fifth slide block is slidably arranged on the fifth slide, the fifth slide block is connected with a disc damping rotating shaft through a bracket, the disc damping rotating shaft is connected with a gear 10, that is, the fifth slide block is connected with a bracket, the bracket is provided with a disc damping rotating shaft, the disc damping rotating shaft is connected with the gear 10, and in the rotating process, the disc damping rotating shaft enables the gear 10 to rotate and stop immediately, so that the self-locking of the gear 10 is realized, and the occurrence of random descending of the boost cylinder 9 is avoided; and when needing to use the boost cylinder 9, with boost cylinder 9 and roof 3 sliding connection, then slide gear 10 to the position that can mesh with pinion rack 10 through the fourth slider, can be through the reciprocating of gear 10 control boost cylinder 9.
Example 5
As shown in fig. 1 and 2, in this embodiment, based on the device of embodiment 1, the needle pushing mechanism is further optimized, the needle pushing mechanism further includes two second connecting plates 12, the two second connecting plates 12 are respectively disposed on two sides of the bracket and are both fixedly connected to the top plate 3 and the bottom plate 1, the second connecting plates 12 are further slidably connected to the first connecting plate 8, that is, the two opposite sides of the second connecting plates 12 are correspondingly provided with vertical second slideways, two sides of the first connecting plate 8 are both provided with second sliders capable of slidably connecting to the second slideways, and the two second connecting plates 12 can prevent the first connecting plate 8 from deflecting during the moving process, so that after the experimental rat is fixed, the injector can be correctly inserted into the rat brain during the downward moving process, thereby reducing the generation of errors;
in order to facilitate the up-and-down movement of the first connecting plate 8, the screw 5 is connected with the top plate 3 through a connecting block, one end of the screw 5 is provided with a second handle, the screw 5 is installed between the two second connecting plates 12 through a bearing, and the screw 5 rotates by rotating the second handle to enable the two connecting rods 7 to move relatively, so that the first connecting plate 8 slides up and down on the second connecting plates 12;
meanwhile, the second connecting plate 12 is provided with scale marks, the syringe is fixed on the first connecting plate 8, namely the distance between the bottom of the syringe needle and the first connecting plate 8 is determined, and the distance for moving the first connecting plate 8 downwards can be determined through the scale marks on the second connecting plate 12, so that the needle of the syringe can be ensured to be inserted into the brain of the rat;
in order to strengthen the fixing effect of the syringe, a fixing mechanism is arranged on the first connecting plate 8, the fixing mechanism is two groups of third slide ways, each third slide way comprises a vertical slide way 13 and a horizontal slide way 14, a first circular through hole 15 is formed in the middle of the first connecting plate 8, the vertical slide ways 13 are arranged on opposite side walls in the first circular through hole 15 and extend from the upper part to the middle part, the horizontal slide ways 14 start from the bottom of the vertical slide ways 13 and extend along the axial direction of the first circular through hole 15, a square clamping groove 16 is communicated below the other end part of the horizontal slide ways 14, a third slide block which is in sliding connection with the third slide ways and can be fixed in the square clamping groove 16 is correspondingly arranged on the syringe, namely when the syringe is installed, the third slide block is firstly inserted into the vertical slide way 13 and moves downwards, and when the syringe is moved to the bottom of the vertical slide way 13, the, the syringe is made to rotate in the horizontal sliding 14, and after the syringe is made to rotate to the end, the syringe moves downwards and is fixed in the square clamping groove 16, at the moment, the syringe cylinder is fixed, the position of the syringe cylinder can be changed by moving the first connecting plate 8, and the piston can be pushed by the boosting cylinder 9 to inject liquid medicine under the condition that the syringe cylinder is not moved.
In specific implementation, firstly, an experimental rat is placed on a fixed table 4 and is fixed by a clamping mechanism, then the fixed table 4 is slid to a proper position and is fixed, after the fixation, the brain of the rat is just positioned below a syringe needle, then a medicine to be injected is sucked in the syringe, a syringe needle cylinder is slidably fixed on a first connecting plate 8, a screw rod 5 is rotated by rotating a second handle to drive a connecting rod 7 to move, the first connecting plate 8 moves downwards, the needle is moved to a position above the brain of the rat, then a boosting cylinder 9 is installed, the bottom of the boosting cylinder 9 is contacted with a piston of the syringe by operating a gear 11, at the moment, the second handle 5 is slowly rotated, the needle of the syringe is determined to be slowly inserted into the brain of the rat according to a scale mark of a second connecting plate 12, then the gear 11 is slowly rotated according to the scale mark on the gear 11, the liquid medicine in the syringe is slowly injected into the brain of the experimental rat, the needle is stopped for a little moment, and then the second handle is operated to pull out the needle of the syringe.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (3)
1. A micro slow positioning injection device for rat brain experiments is characterized by comprising a bracket, a needle pushing mechanism and a needle core pushing mechanism;
the support comprises a bottom plate (1), four telescopic supporting columns (2) uniformly arranged at the top of the bottom plate (1) and a top plate (3) jointly connected with the four telescopic supporting columns (2), a fixed table (4) is further arranged on the bottom plate (1) in a sliding mode, a clamping mechanism is arranged on the fixed table (4) in a sliding mode, the fixed table (4) comprises a fixed plate and a first sliding block located at the bottom of the fixed plate, a first sliding way capable of being connected with the first sliding block in a sliding mode is formed in the bottom plate (1), and a fixing bolt is further arranged on the first sliding block;
the needle pushing mechanism comprises a screw rod (5) fixedly arranged below the top plate (3), two thread sleeves (6) symmetrically arranged on the screw rod (5), a connecting rod (7) hinged on the thread sleeves (6), a first connecting plate (8) jointly hinged with the two connecting rods (7) and a fixing mechanism arranged in the middle of the first connecting plate (8) and used for fixing a syringe needle cylinder, wherein the screw rod (5) consists of two sections which are integrally formed, but the spiral directions of threads on the two sections are opposite, the needle pushing mechanism further comprises two second connecting plates (12), the two second connecting plates (12) are respectively arranged on two sides of the support and are fixedly connected with the top plate (3) and the bottom plate (1), and the second connecting plates (12) are also in sliding connection with the first connecting plates (8), two opposite side surfaces of the second connecting plate (12) are correspondingly provided with second slide ways in the vertical direction, two second slide blocks capable of being connected with the second slide ways in a sliding manner are arranged on two sides of the first connecting plate (8), scale marks are arranged on the second connecting plate (12), the fixing mechanism is two groups of third slide ways, each third slide way comprises a vertical slide way (13) and a horizontal slide way (14), a first circular through hole (15) is formed in the middle of the first connecting plate (8), the vertical slide ways (13) are arranged on opposite side walls in the first circular through hole (15) and extend to the middle from the upper part, the horizontal slide ways (14) start from the bottom of the vertical slide ways (13) and extend along the axial direction of the first circular through hole (15), and a square clamping groove (16) is communicated and arranged below the other end part of the horizontal slide way (14), a third sliding block which is in sliding connection with the third slide way and can be fixed in the square clamping groove (16) is correspondingly arranged on the syringe needle cylinder;
the stylet pushing mechanism comprises a boosting cylinder (9) which penetrates through the top plate (3) and is in sliding connection, a toothed plate (10) which is fixedly arranged at the top of the boosting cylinder (9), and a gear (11) which is meshed with the toothed plate (10) and can be self-locked, the gear (11) is arranged on the top plate (3) in a sliding manner, a first handle and scale marks are arranged on the gear (11), a fifth slideway is arranged on the top plate (3), a fifth slide block is arranged on the fifth slideway in a sliding way, the fifth slide block is connected with a disc damping rotating shaft through a bracket, the disc damping rotating shaft is connected with the gear (11), a second round through hole which can accommodate the boosting cylinder is arranged in the middle of the top plate (3), vertical fourth slide ways with convex-shaped cross sections are arranged on two opposite sides in the second round through hole, and fourth sliding blocks which can be in sliding connection with the fourth sliding ways are correspondingly arranged on two sides of the boosting cylinder (9).
2. The rat brain experiment microscale slow-positioning injection device of claim 1, wherein the clamping mechanism is two clamping plates slidably disposed on the fixing plate.
3. The rat brain experiment micro slow positioning injection device according to claim 1, wherein the screw rod (5) is connected with the top plate (3) through a connecting block, a second handle is arranged at one end of the screw rod (5), and the screw rod (5) is installed between the two second connecting plates (12) through a bearing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910199489.2A CN109730800B (en) | 2019-03-15 | 2019-03-15 | Slow injection device that fixes a position of trace for rat brain experiments |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910199489.2A CN109730800B (en) | 2019-03-15 | 2019-03-15 | Slow injection device that fixes a position of trace for rat brain experiments |
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| CN109730800A CN109730800A (en) | 2019-05-10 |
| CN109730800B true CN109730800B (en) | 2021-02-23 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110974476A (en) * | 2019-11-22 | 2020-04-10 | 张珂孟 | Injection equipment that animal was used |
| CN110974477B (en) * | 2019-11-22 | 2022-10-28 | 张珂孟 | Injection device that animal husbandry used |
| CN112043455A (en) * | 2020-08-04 | 2020-12-08 | 湖州华嘉特种养殖有限公司 | Poultry is bred and uses bacterin injection auxiliary device |
| CN112168414B (en) * | 2020-10-20 | 2022-10-25 | 河南省中医院（河南中医药大学第二附属医院） | Injection device for rat brain experiments |
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| CN208511244U (en) * | 2017-12-12 | 2019-02-19 | 延安大学 | A kind of cerebral injury Postural immobilization support device |
| CN108187195A (en) * | 2017-12-18 | 2018-06-22 | 陈文华 | A kind of clinic's patient infusion arm auxiliary fixing device |
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