CN206007402U - Experiment mice double-cylinder type heart perfusion device - Google Patents
Experiment mice double-cylinder type heart perfusion device Download PDFInfo
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- CN206007402U CN206007402U CN201620714385.2U CN201620714385U CN206007402U CN 206007402 U CN206007402 U CN 206007402U CN 201620714385 U CN201620714385 U CN 201620714385U CN 206007402 U CN206007402 U CN 206007402U
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- perfusion
- cylinder
- experiment mice
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- piston
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Abstract
The utility model discloses a kind of experiment mice double-cylinder type heart perfusion device, which includes pedestal, two perfusion cylinders, two driven gears, X-type four-way conduit and spring clips, wherein, is fixed with stent strut on pedestal；Two perfusion cylinders are fixedly connected with the stent strut respectively, are equipped with piston handle in each perfusion cylinder, and its one end that gos deep in the perfusion cylinder is provided with piston, and the one side of the piston handle is provided with tooth bar；Two driven gears are fixedly connected with the stent strut respectively, and each gear mutually can be closely engaged with the tooth bar on corresponding piston handle, and the driven gear center is provided with axle, and axle connection manual rotating handle；The pinion rotation can be driven by rotating the manual rotating handle, and then drive the piston handle movement；The two of which port of X-type four-way conduit can by flexible rubber hose respectively with described two perfusion cylinders two head end close communications, two other port is connected with transfusion needle respectively；Spring clip is used for the hose for clamping the transfusion needle.
Description
Technical field
The utility model is related to a kind of doctor, pharmacy teaching and scientific experiment apparatus, and more particularly to a kind of experiment mice is used
Double-cylinder type heart perfusion device.
Background technology
In the experimental study of experiment mice, need to observe medicine under experiment mice physiological status or under pathological state
The change of morphosis, for example with observation by light microscope hematoxylin eosin staining and the histiocytic knot of congo red staining
Structure changes, and using the histiocytic Change of Ultrastructure of transmission electron microscope observation, is seen using immunohistochemistry staining method
The expressive site or expression of certain albumen histocyte Nei is examined, these are required to the interference for excluding blood in experiment mice body,
Interference of tissue cell necrosis etc. after mouse is drawn materials will also be excluded.And be conducive to the conventional side of experiment mice Observation On The Morphology
Method is the perfusion of experiment mice heart in vivo, it is seen that heart perfusion acts on observation morphosis after experiment mice for research medicine
Change particularly important.
It is exactly heart perfusion device that experiment carries out heart in vivo and irrigates selected instrument to mouse, but existing heart perfusion
Device has that infusion time length, complex operation and efficiency are low.
Content of the invention
The technical problems to be solved in the utility model be to provide a kind of simple structure, low cost, test easily to operate little
Mouse heart irrigator, its are substantially shorter infusion time, improve charging efficiency.
Be the experiment mice of the present utility model double-cylinder type heart perfusion device up to above-mentioned purpose, it include fixed seat, two
Perfusion cylinder, two driven gears, X-type four-way conduit and spring clips, wherein, fix and are fixed with stent strut；Two perfusion cylinders point
It is not fixedly connected with the stent strut, in each perfusion cylinder, is equipped with piston handle, its one end that gos deep in the perfusion cylinder is provided with
Piston, and the one side of the piston handle is provided with the jagged tooth bar of a row；Two driven gears are solid with the stent strut respectively
Fixed connection, each gear mutually can be closely engaged with the tooth bar on corresponding piston handle, and the driven gear center is provided with axle, and
The axle connects manual rotating handle；The pinion rotation can be driven by rotating the manual rotating handle, and then drive the piston handle
Mobile；The two of which port of X-type four-way conduit can be tight with two head ends of described two perfusion cylinders respectively by flexible rubber hose
Connection, two other port is connected with transfusion needle respectively；Spring clip is used for the hose for clamping the transfusion needle.
Preferably, the perfusion cylinder is marked with the scale for representing volume.
Preferably, one end that the piston handle is exposed to outside the perfusion cylinder is provided with pressing plate.
Preferably, the irrigator also includes sealed ubber strip, for sealing two ports and the flexible glue of X-type four-way conduit
Pipe and flexible rubber hose with described two perfusion cylinders two head ends junction.
Preferably, the fixed seat is metal platform.
The utility model difference from prior art is that the utility model achieves following technique effect：
There is zigzag tooth bar the piston handle side of the utility model heart perfusion device, and which is mutually closely stung with driven gear
Close, the center of driven gear has axle, axle to connect manual rotating handle, and this being designed with is beneficial at the uniform velocity, controllably carry out experiment mice
Heart perfusion, while being designed with beneficial to the strength for saving experimenter for manual rotating handle, is conducive to strengthening novel experimental mouse use
The effect of heart perfusion device experimental implementation.
In the utility model, experimenter can drive the rotation of driven gear by rotation manual swing handle.Due to passive
Gear accurately, is closely engaged with the tooth bar of piston handle, and therefore experimenter causes the position of piston handle by the rotation of driven gear
Put and change, piston handle promotes the perfusion liquid such as the physiological saline irrigated in cylinder, paraformaldehyde, glutaraldehyde to flow out perfusion cylinder,
Perfusion liquid is entered in the blood circulation system of experiment mice by flexible rubber hose, X-type four-way conduit, transfusion needle.
In the utility model, 2 perfusion cylinders are have also been devised, the head end of each perfusion cylinder is connected with flexible rubber hose respectively, two
Flexible rubber hose connects the two-port of X-type four-way conduit respectively, and the two ends of flexible rubber hose have sealed ubber strip to seal.X-type four-way
Two other port of conduit connects small size transfusion needle respectively.These are designed with beneficial to double-cylinder type heart perfusion device to experiment mice
Experiment mice heart perfusion paraformaldehyde (or glutaraldehyde) can be carried out continuously after heart perfusion physiological saline, greatly improve this
The efficiency of secondary experiment heart perfusion.In the experiment of experiment mice heart perfusion, if selecting physiological saline to carry out experiment mice
Heart perfusion, it is only necessary to which spring clip is clamped the small size transfusion needle containing paraformaldehyde (or glutaraldehyde) liquid, so can be with
Thoroughly the blood in the scouring experiment mouse circulatory system, reduces blood to hematoxylin eosin staining, congo red staining, immune group
The interference of multiple dyeing such as weave chemistry dyeing.And need selection to carry out the heart perfusion of paraformaldehyde (or glutaraldehyde) liquid,
Only needing to for spring clip to clamp the small size transfusion needle containing physiological saline i.e. can be with.It can be seen that the utility model be conducive to haematine-
Eosin stains, congo red staining, immunohistochemical staining, transmission electron microscope observation ultra microstructure etc., indicate this reality
Preferable with new operability.
Overall merit, very experiment mice of the present utility model double-cylinder type heart perfusion device good stability, laborsaving, perfusion
Efficiency high, simple structure, cheap, and can also ensure to irrigate liquid slowly, at the uniform velocity, precisely controllable to experiment mice
Heart perfusion is carried out, is conducive to subsequent experimental such as the morphology optical microscope and transmitted electron of experiment mice heart perfusion to show
Micro mirror observation etc. is smoothly carried out.
It is described further to of the present utility model below in conjunction with the accompanying drawings.
Description of the drawings
Fig. 1 is the structural representation of experiment mice double-cylinder type heart perfusion device in one embodiment of the utility model；
Fig. 2 is the mplifying structure schematic diagram of X-type four-way conduit in Fig. 1.
Description of reference numerals：
(the heart perfusion device in the embodiment of Fig. 1 and Fig. 2 is made up of two groups of identical devices, for convenience of and clear for the sake of,
The formal notation of identical part n, n ' in two groups.)
10- fixed seats；101- stent struts；
11st, 11 '-perfusion cylinder；111st, 111 '-piston handle；112nd, 112 '-piston；113rd, 113 '-tooth bar；114th, 114 '-pressure
Plate；
12nd, 12 '-driven gear；121st, 121 '-axle；122nd, 122 '-manual rotating handle；
13-X type four-way conduits；131st, 131 '-upside port；132nd, 132 '-downside port；
14- spring clips；15th, 15 '-flexible rubber hose；16th, 16 '-transfusion needle；17th, 17 '-the first sealed ubber strip；18th, 18 '-length
Plate；19th, 19 '-short slab；20th, 20 '-the second sealed ubber strip.
Specific embodiment
With reference to embodiments, the above-mentioned He other technical characteristic of the utility model and advantage are made in more detail
Bright.
Fig. 1 is referred to, experiment mice of the present utility model double-cylinder type heart perfusion device, including the fixation that metal platform is formed
Seat 10, two perfusions cylinder (11,11 '), two driven gears (12,12 '), X-type four-way conduit 13 and spring clips 14.
Wherein, stent strut 101 is fixed with fixed seat 10.
Two perfusion cylinders 11,11 ' are marked with the scale for representing volume, in order to draw and inject the liquid of certain volume
(such as physiological saline, paraformaldehyde or glutaraldehyde).Perfusion cylinder 11,11 ' is fixedly connected with stent strut 101 by long slab 18,18 ',
Piston handle 111,111 ' is provided with perfusion cylinder 11,11 ', its one end deeply irrigated in cylinder is provided with piston 112,112 ', and piston
The one side of handle is provided with the jagged tooth bar 113,113 ' of a row.In addition, piston handle 111,111 ' is exposed to outside perfusion cylinder
End is provided with pressing plate 114,114 ', and the design of pressing plate can be easy to people to discharge gas or liquid in perfusion cylinder manually.In addition, perfusion cylinder
It is fixed on the stent strut of metal platform by long slab, this enhances the stability of heart perfusion effect, it is to avoid experimental implementation mistake
The experiment effect of heart perfusion is affected because of the shake of hand in journey, be conducive to smoothly entering for experiment mice heart perfusion experiment
OK.
Two driven gears 12,12 ' are fixedly connected with stent strut 101 respectively by short slab 19,19 ', and each gear can be with
Tooth bar on corresponding piston handle is mutually closely engaged, and the difference of driven gear 12,12 ' mutually can be closely stung with tooth bar 113,113 '
Close.Driven gear 12,12 ' centers are provided with axle 121,121 ', and the connection manual rotating handle 122,122 ' of axle 121,121 '；By rotation
Manual rotating handle 122,122 ' can be rotated with moving gear 12,12 ', and then drives piston handle 111,111 ' mobile.
The two of which port of X-type four-way conduit 13, i.e. upside port 131,131 ' can be by flexible rubber hose 15,15 ' respectively
With perfusion cylinder 11,11 ' two head end close communications, and in junction arrange the first sealed ubber strip 17,17 '；And, flexible rubber hose
15th, the junction of 15 ' another and upside port 131,131 ' is provided with the second sealed ubber strip 20,20 '.Two other port,
That is downside port 132,132 ' is connected with transfusion needle 16,16 ' respectively, as shown in Figure 2.
Spring clip 14 is used for the hose for clamping transfusion needle.
In said structure, in perfusion cylinder, piston handle is set, piston handle deeply irrigates the one end in cylinder and sets piston and piston handle
The other end sets pressing plate, in combination with the use of sealed ubber strip, it is ensured that the sealing of heart perfusion effect, so as to ensure to test
The smooth development of mouse heart perfusion.
The use idiographic flow of the utility model double-cylinder type heart perfusion device is as follows：
The sealed ubber strip 17 on flexible rubber hose 15 is removed, manual rotating handle 122 is moved in dextrorotation, and perfusion cylinder 11 passes through piston handle
111 draw 100ml physiological saline, install flexible rubber hose 15 and sealed ubber strip 17.Remove the sealed ubber strip on flexible rubber hose 15 '
17 ', turn manual rotating handle 122 ' counterclockwise, perfusion cylinder 11 ' draw 100ml paraformaldehydes (or penta 2 by piston handle 111 '
Aldehyde), flexible rubber hose 15 ' and sealed ubber strip 17 ' are installed.
The hose that small size transfusion needle 16 ' is clamped using spring clip 14, turn manual rotating handle 122 counterclockwise drive passive tooth
Wheel 12 is rotated, and driven gear 12 drives piston handle 111 to move downwards, and physiological saline passes through flexible rubber hose 15, X-type four-way conduit 13
And 10ml physiological saline discharged by small size transfusion needle 16, be conducive to washing away double-cylinder type heart perfusion device liquid outlet and discharging air.
Using the most obvious position of the left ventricle apex beat of the piercing experiment mice of small size transfusion needle 16, fixed trumpet transfusion needle
At 16 needle handle, experiment mice right auricle of heart is cut off, turn manual rotating handle 122 counterclockwise drives driven gear 12 to rotate, passive tooth
Wheel 12 drives piston handle 111 to move downwards, and physiological saline is by flexible rubber hose 15, X-type four-way conduit 13 and small size transfusion needle 16
Discharge, until experiment mice liver bleaches and right auricle of heart outflow clear liquid, show that experiment mice blood circulation system is thorough
Wash away.
The hose that small size transfusion needle 16 is clamped using spring clip 14, is pierced into experiment mice using small size transfusion needle 16 '
The most obvious position of left ventricle apex beat, at the needle handle of fixed trumpet transfusion needle 16 ', the dynamic manual rotating handle 122 ' of dextrorotation drives
Driven gear 12 ' is rotated, and driven gear 12 ' drives piston handle 111 ' to move downwards, by flexible rubber hose 15 ', X-type four-way conduit
13rd, paraformaldehyde (or glutaraldehyde) liquid 70ml discharged by small size transfusion needle 16 ', till experiment mice four limbs are stiff, shows
Experiment mice heart perfusion success.
Embodiment described above is only that preferred embodiment of the present utility model is described, not to this practicality
New scope is defined, and on the premise of without departing from the utility model design spirit, those of ordinary skill in the art are to this
Various modifications and improvement that the technical scheme of utility model is made, all should fall into the protection of the utility model claims determination
In the range of.
Claims (5)
1. a kind of experiment mice double-cylinder type heart perfusion device, it is characterised in that include：
Fixed seat, is fixed with stent strut thereon；
Two perfusion cylinders, which is fixedly connected with the stent strut, is equipped with piston handle in each perfusion cylinder, and which gos deep into the perfusion
One end in cylinder is provided with piston, and the one side of the piston handle is provided with the jagged tooth bar of a row；
Two driven gears, which is fixedly connected with the stent strut, and each gear can be mutual with the tooth bar on corresponding piston handle
Closely it is engaged, the driven gear center is provided with axle, and axle connection manual rotating handle；Can band by rotating the manual rotating handle
The pinion rotation is moved, and then drives the piston handle movement；
X-type four-way conduit, two of which port can closely be connected with two head ends of described two perfusion cylinders respectively by flexible rubber hose
Logical, two other port is connected with transfusion needle respectively；And
Spring clip, for clamping the hose of the transfusion needle.
2. experiment mice according to claim 1 double-cylinder type heart perfusion device, it is characterised in that the perfusion cylinder subscript
There is the scale for representing volume.
3. experiment mice according to claim 1 double-cylinder type heart perfusion device, it is characterised in that the piston handle exposes
One end outside the perfusion cylinder is provided with pressing plate.
4. experiment mice according to claim 1 double-cylinder type heart perfusion device, it is characterised in that the irrigator is also wrapped
Sealed ubber strip is included, for two ports of the X-type four-way conduit being sealed with flexible rubber hose and flexible rubber hose and described two perfusions
The junction of two head ends of cylinder.
5. experiment mice according to claim 1 double-cylinder type heart perfusion device, it is characterised in that the fixed seat is gold
Category platform.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620714385.2U CN206007402U (en) | 2016-07-07 | 2016-07-07 | Experiment mice double-cylinder type heart perfusion device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620714385.2U CN206007402U (en) | 2016-07-07 | 2016-07-07 | Experiment mice double-cylinder type heart perfusion device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206007402U true CN206007402U (en) | 2017-03-15 |
Family
ID=58243559
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620714385.2U Expired - Fee Related CN206007402U (en) | 2016-07-07 | 2016-07-07 | Experiment mice double-cylinder type heart perfusion device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206007402U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109730800A (en) * | 2019-03-15 | 2019-05-10 | 河南医学高等专科学校 | Micro slow positioning injection device is used in a kind of experiment of rat brain |
-
2016
- 2016-07-07 CN CN201620714385.2U patent/CN206007402U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109730800A (en) * | 2019-03-15 | 2019-05-10 | 河南医学高等专科学校 | Micro slow positioning injection device is used in a kind of experiment of rat brain |
| CN109730800B (en) * | 2019-03-15 | 2021-02-23 | 河南医学高等专科学校 | Slow injection device that fixes a position of trace for rat brain experiments |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170315 Termination date: 20190707 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |