CN109730307A - The composite nutrient and the preparation method and application thereof for preventing and treating hypertension, hyperlipemia disease - Google Patents
The composite nutrient and the preparation method and application thereof for preventing and treating hypertension, hyperlipemia disease Download PDFInfo
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Abstract
The invention discloses a kind of composite nutrients for preventing and treating hypertension, hyperlipemia disease, including hawthorn, sanchi flower, Camellia nitidissima, corn stigma, balsam pear, Black Box Tracing berry, raffinose-oligosaccharide, egg white fermentation material, fermented ginseng powder, wheat oligopeptide, olive fruit powder, grape seed extract, black garlic powder etc., nutrient the preparation method comprises the following steps: by hawthorn, sanchi flower, Camellia nitidissima crush, pectase and papain enzymolysis is added, it is refrigerated after enzyme deactivation, add water pressurization impregnate, take filtrate dry solid mixture A；Corn stigma, balsam pear, Black Box Tracing berry are crushed, Lactobacillus saki is added, ferments under infrared radiation, ethyl alcohol is added after sterilizing, is freezed, water-bath takes filtrate to be dried to obtain solid mixture B；Solid mixture A, solid mixture B, other raw materials are mixed；Also disclose application of the composite nutrient in the product for preparing preventing hypertension hyperlipidemia.Product of the invention has the beneficial effect of adjusting hypertension and hyperlipidemia.
Description
Technical field
The present invention relates to nutrition and field of functional food.It is more particularly related to which a kind of prevention and treatment hypertension is high
The composite nutrient and the preparation method and application thereof of blood lipid disease.
Background technique
With the development and improvement of living standard of society and the change of living habit, the lipid material of intake is got over
Come more, be easy to cause hypertension and hyperlipemia for a long time, hypertension is difficult thoroughly to cure, can only by long-term medication into
Row inhibits, the very big side effect of health bad student of the Long-term taking medicine to people, however is once discontinued, and will cause rebound, therefore,
Be badly in need of research and development it is a be not drug product, the hypertension, hyperlipemia disease of crowd can be alleviated.
Summary of the invention
It is an object of the invention to solve at least the above problems, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of composite nutrients for preventing and treating hypertension, hyperlipemia disease, have prevention and treatment high
The effect of blood pressure provides a kind of preparation method of composite nutrient for preventing and treating hypertension, hyperlipemia disease, with depth extraction and can protect
It keeps hawthorn, sanchi flower, Camellia nitidissima general flavone here, and makes final products that there is certain radiation resistance.
In order to realize these purposes and other advantages according to the present invention, a kind of prevention and treatment hypertension, hyperlipemia disease is provided
Composite nutrient, including following parts by weight of component are made:
1.8 parts of hawthorn, 5 parts of sanchi flower, 5 parts of Camellia nitidissima, 2 parts of corn stigma, 8 parts of balsam pear, 6 parts of Black Box Tracing berry,
0.2 part of raffinose-oligosaccharide, 5 parts of egg white fermentation material, 2 parts of fermented ginseng powder, 0.2 part of wheat oligopeptide, 0.01 part of olive fruit powder, Portugal
0.05 part of grape seed extract, 0.1 part of black garlic powder, 0.01 part of theanine, 0.01 part of γ-aminobutyric acid, 0.02 part of L-arabinose.
Preferably, the egg white fermentation material the preparation method comprises the following steps:
Step 1: egg white pre-processes: it is in mass ratio that 1:0.02:0.08:5 is mixed by egg white, mushroom taro powder, deionized water,
It is 10 DEG C in temperature, mixing speed is that 30min is stirred under 600rpm, and sodium dihydrogen phosphate and jackfruit seed extract, Yu Wen is added
10 DEG C of degree, mixing speed are to stir 3min under 100rpm, refrigerate 2 months at less than 10 DEG C, obtain refrigeration egg white；
Wherein, egg white, sodium dihydrogen phosphate, jackfruit seed extract mass ratio be 1:0.05:0.12；
Jackfruit seed extract the preparation method comprises the following steps: jackfruit seed is taken to be crushed to grain diameter less than 25 μm, light soy sauce is added
With chitosan in microwave power be 500W under microwave treatment 5min, then be added mass fraction be 95% ethyl alcohol soaked at 50 DEG C
3h is mentioned, filtrate is filtered to take and is concentrated to get jackfruit seed extract after volatilization removes ethyl alcohol；Wherein, jackfruit seed, light soy sauce, shell are poly-
Sugared, ethyl alcohol mass ratio is 0.12:0.004:0.002:1；
Step 2: enzymolysis processing: in the refrigeration egg white into step 1 be added protease 4h is digested at 15 DEG C, then plus
Enter compound bacteria to ferment at 15 DEG C 50min, being placed in magnetic field strength is to act on 15min under 1T, then freezed 1 month at -18 DEG C,
Egg white must be freezed, after frozen egg removing summer-heat is frozen, carry out micro porous filtration, the aperture of micropore filtering film is 10 μm, retain filter upper object and
Filter lower object；Wherein, refrigerate egg white, protease, compound bacteria mass ratio be 1:0.02:0.04, compound bacteria tieed up by Marx's Crewe
Yeast, lactobacillus reuteri, staphylococcus xylosus are 1:1.2:1.5 composition in mass ratio；
Step 3: acidolysis is handled: lactic acid is added in object in the filter into step 2, is acidolysis 10d at 10 DEG C in temperature, carries out
Micro porous filtration, the aperture of micropore filtering film are 10 μm, retain and filter lower object；Wherein, in the filter in step 2 object, lactic acid mass ratio
For 1:0.3；
Step 4: merge object under the filter under the filter in step 2 in object and step 3, be then added oligofructose and go from
Sub- water is to stir 10min under 300rpm in mixing speed, is drying to obtain egg white fermentation material；Wherein, under the filter in step 2 object and
The mass ratio of the quality summation, oligofructose, deionized water of object is 1:0.05:0.2 under filter in step 3.
A kind of preparation method of composite nutrient for preventing and treating hypertension, hyperlipemia disease is provided, comprising:
A, hawthorn, sanchi flower, Camellia nitidissima are mixed and is crushed, pectinase enzymatic hydrolysis 1h is then added, pawpaw egg is then added
White enzyme digests 5h, after enzyme deactivation, is placed at 10 DEG C and refrigerates 5h, adds the water of 500 times of hawthorn quality, after stirring 20min, in
Under 10MPa pressurization impregnate 8h, filter to take filtrate, by filtrate it is dry solid mixture A, wherein the quality of pectase be hawthorn
The 2% of quality, the quality of papain are the 1.2% of hawthorn quality；
B, corn stigma, balsam pear, Black Box Tracing berry are mixed and is crushed, Lactobacillus saki is added, it is at 28 DEG C and red
The lower 12h that ferments of outside line irradiation, after sterilizing, is added the ethyl alcohol that the mass fraction of 100 times of corn stigma quality is 95%, stirring, Yu Wen
Degree is -10 DEG C of freezing 12h, then in 55 DEG C of heating water bath 5h, filters to take filtrate, after filtrate recycling ethanol, is dried to obtain solid
Mixture B；
C, by the solid mixture A in step a, the solid mixture B in step b, raffinose-oligosaccharide, egg white fermentation material,
Fermented ginseng powder, wheat oligopeptide, olive fruit powder, grape seed extract, black garlic powder, theanine, γ-aminobutyric acid, L- I
Primary sugar mixes up to nutrient.
Preferably, the temperature of the 1h of pectinase enzymatic hydrolysis is 35 DEG C in step a, and the temperature of papain enzymolysis is 45
℃。
Preferably, the intensity of infrared radiation is 100J/cm in step b2。
Preferably, the inoculum concentration of Lactobacillus saki is the 0.6% of corn stigma quality in step b.
Preferably, fermented ginseng powder the preparation method comprises the following steps: take fresh ginseng, 20-30 DEG C of water temperature cleaning uses mashing mechanism
Pulp obtains ginseng pulp；Into ginseng pulp plus water, amount of water are 40 times of volumes of ginseng pulp, cross colloid mill, obtain ginseng liquid；By people
Ginseng liquid is heated to 40 DEG C, and zymophyte is added, and fermentation bacterium amount, which is behaved, joins 0.05 times of quality of liquid, and zymophyte is Bulgarian Lactobacillus
With streptococcus thermophilus 1:1 mass ratio, ferment 10h, decomposes polysaccharose substance, and ginsenoside is precipitated, obtains fermented ginseng stoste；Freezing
Dry fermented ginseng powder.
Application of the nutrient in the product for preparing preventing hypertension.
Nutrient is preparing the application in anti-radiation product.
The present invention is include at least the following beneficial effects: the composite nutrient of prevention and treatment hypertension, hyperlipemia disease not only has anti-
Control the effect of hypertension, preparation method with depth extraction and can retain hawthorn, sanchi flower, Camellia nitidissima general flavone.
Further advantage, target and feature of the invention will be partially reflected by the following instructions, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be noted that experimental method described in following embodiments is unless otherwise specified conventional method, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
<embodiment 1>
The composite nutrient of hypertension, hyperlipemia disease is prevented and treated, including following parts by weight of component is made:
1.8 parts of hawthorn, 5 parts of sanchi flower, 5 parts of Camellia nitidissima, 2 parts of corn stigma, 8 parts of balsam pear, 6 parts of Black Box Tracing berry,
0.2 part of raffinose-oligosaccharide, 5 parts of egg white fermentation material, 2 parts of fermented ginseng powder, 0.2 part of wheat oligopeptide, 0.01 part of olive fruit powder, Portugal
0.05 part of grape seed extract, 0.1 part of black garlic powder, 0.01 part of theanine, 0.01 part of γ-aminobutyric acid, 0.02 part of L-arabinose.
Preparation method, comprising:
A, hawthorn, sanchi flower, Camellia nitidissima are mixed and is crushed, pectinase enzymatic hydrolysis 1h is then added, pawpaw egg is then added
White enzyme digests 5h, after enzyme deactivation, is placed at 10 DEG C and refrigerates 5h, adds the water of 500 times of hawthorn quality, after stirring 20min, in
Under 10MPa pressurization impregnate 8h, filter to take filtrate, by filtrate it is dry solid mixture A, wherein the quality of pectase be hawthorn
The 2% of quality, the quality of papain are the 1.2% of hawthorn quality；
B, corn stigma, balsam pear, Black Box Tracing berry are mixed and is crushed, Lactobacillus saki is added, it is at 28 DEG C and red
The lower 12h that ferments of outside line irradiation, after sterilizing, is added the ethyl alcohol that the mass fraction of 100 times of corn stigma quality is 95%, stirring, Yu Wen
Degree is -10 DEG C of freezing 12h, then in 55 DEG C of heating water bath 5h, filters to take filtrate, after filtrate recycling ethanol, is dried to obtain solid
Mixture B；
C, by the solid mixture A in step a, the solid mixture B in step b, raffinose-oligosaccharide, egg white fermentation material,
Fermented ginseng powder, wheat oligopeptide, olive fruit powder, grape seed extract, black garlic powder, theanine, γ-aminobutyric acid, L- I
Primary sugar mixes up to nutrient.
The temperature of the 1h of pectinase enzymatic hydrolysis is 35 DEG C in step a, and the temperature of papain enzymolysis is 45 DEG C.
The intensity of infrared radiation is 100J/cm in step b2。
The inoculum concentration of Lactobacillus saki is the 0.6% of corn stigma quality in step b.
Fermented ginseng powder the preparation method comprises the following steps: take fresh ginseng, 20-30 DEG C of water temperature cleaning is slurried using beater, is obtained
Ginseng pulp；Into ginseng pulp plus water, amount of water are 40 times of volumes of ginseng pulp, cross colloid mill, obtain ginseng liquid；Ginseng liquid is heated to
40 DEG C, zymophyte is added, fermentation bacterium amount, which is behaved, joins 0.05 times of quality of liquid, and zymophyte is Bulgarian Lactobacillus and thermophilus
Bacterium 1:1 mass ratio, ferment 10h, decomposes polysaccharose substance, and ginsenoside is precipitated, obtains fermented ginseng stoste；It is freeze-dried to obtain ginseng
Yeast powder.
<embodiment 2>
The preparation method is the same as that of Example 1 for the composite nutrient of prevention and treatment hypertension, hyperlipemia disease, wherein unlike, egg white hair
Ferment object the preparation method comprises the following steps:
Step 1: egg white pre-processes: it is in mass ratio that 1:0.02:0.08:5 is mixed by egg white, mushroom taro powder, deionized water,
It is 10 DEG C in temperature, mixing speed is that 30min is stirred under 600rpm, and sodium dihydrogen phosphate and jackfruit seed extract, Yu Wen is added
10 DEG C of degree, mixing speed are to stir 3min under 100rpm, refrigerate 2 months at less than 10 DEG C, obtain refrigeration egg white；
Wherein, egg white, sodium dihydrogen phosphate, jackfruit seed extract mass ratio be 1:0.05:0.12；
Jackfruit seed extract the preparation method comprises the following steps: jackfruit seed is taken to be crushed to grain diameter less than 25 μm, light soy sauce is added
With chitosan in microwave power be 500W under microwave treatment 5min, then be added mass fraction be 95% ethyl alcohol soaked at 50 DEG C
3h is mentioned, filtrate is filtered to take and is concentrated to get jackfruit seed extract after volatilization removes ethyl alcohol；Wherein, jackfruit seed, light soy sauce, shell are poly-
Sugared, ethyl alcohol mass ratio is 0.12:0.004:0.002:1；
Step 2: enzymolysis processing: in the refrigeration egg white into step 1 be added protease 4h is digested at 15 DEG C, then plus
Enter compound bacteria to ferment at 15 DEG C 50min, being placed in magnetic field strength is to act on 15min under 1T, then freezed 1 month at -18 DEG C,
Egg white must be freezed, after frozen egg removing summer-heat is frozen, carry out micro porous filtration, the aperture of micropore filtering film is 10 μm, retain filter upper object and
Filter lower object；Wherein, refrigerate egg white, protease, compound bacteria mass ratio be 1:0.02:0.04, compound bacteria tieed up by Marx's Crewe
Yeast, lactobacillus reuteri, staphylococcus xylosus are 1:1.2:1.5 composition in mass ratio；
Step 3: acidolysis is handled: lactic acid is added in object in the filter into step 2, is acidolysis 10d at 10 DEG C in temperature, carries out
Micro porous filtration, the aperture of micropore filtering film are 10 μm, retain and filter lower object；Wherein, in the filter in step 2 object, lactic acid mass ratio
For 1:0.3；
Step 4: merge object under the filter under the filter in step 2 in object and step 3, be then added oligofructose and go from
Sub- water is to stir 10min under 300rpm in mixing speed, is drying to obtain egg white fermentation material；Wherein, under the filter in step 2 object and
The mass ratio of the quality summation, oligofructose, deionized water of object is 1:0.05:0.2 under filter in step 3.
<comparative example 1>
The preparation method of nutrient is the same as embodiment 2, wherein and step a is different, specifically: by hawthorn, sanchi flower, Camellia nitidissima
It mixes and crushes, the water of 500 times of hawthorn quality is added, after stirring 20min, impregnate 8h, filter to take filtrate, filtrate is admittedly dry
Body mixture A.
<comparative example 2>
The preparation method of nutrient is the same as embodiment 2, wherein and step b is different, specifically: by corn stigma, balsam pear, black fruit gland
Rib rowanberry is mixed and is crushed, and the ethyl alcohol that the mass fraction of 100 times of corn stigma part quality is 95%, stirring, Yu Changwen is added
Lower standing 12h filters to take filtrate then in 55 DEG C of heating water bath 5h, after filtrate recycling ethanol, is dried to obtain solid mixture B.
<comparative example 3>
The preparation method of egg white fermentation material is the same as embodiment 2, wherein does not pre-process to egg white, directly carries out at enzymatic hydrolysis
Reason.
<comparative example 4>
The preparation method of egg white fermentation material is the same as embodiment 2, wherein step 2 is different, and in step 2, frozen egg removing summer-heat is frozen
Afterwards, micro porous filtration, directly the acidolysis processing in progress step 3 are not carried out.
<evaluation test of nutrient preparation method>
1, the content for detecting the general flavone in the solid mixture A prepared in the method for embodiment 1 and comparative example 1, such as table 1
It is shown:
Table 1
As seen from the above table, solid mixture A is prepared using the method for embodiment 1, the content of general flavone can be significantly improved,
Illustrate to digest hawthorn, sanchi flower, Camellia nitidissima in advance using pectase, cell wall can be destroyed, increase the permeability of cell wall,
The dissolution for facilitating content digests together in conjunction with papain, can be to a small amount of egg contained in itself in material composition
White matter is decomposed, and to release the small-molecule substance being incorporated in protein, promotes the outflow of Flavonoid substances, refrigeration and pressurization
Effect accelerates Flavonoid substances dissolution in a solvent, the final content for improving general flavone in solid mixture A.
<nutrient evaluation test>
1, angiotensin converting enzyme ACE inhibits test
It is Angiotensin II that angiotensin converting enzyme (ACE), which can be catalyzed angiotensin I converting, causes institute's liter
It is high.Inhibit the activity of ACE to make the generation of Angiotensin II reduce, while reduce bradykinin inactivation, to reach suppression
Blood pressure processed increases, the purpose of prevention and treatment hypertension.
This test detects nutrition prepared by embodiment 1, embodiment 2, comparative example 2 using Hippuryl-His-Leu as substrate
Element to the inhibitory activity of ACE, reaction system include boric acid-borate buffer solution of PH8.3,50 μ L 5mmol/L Hippuryl-
His-Leu, the nutrient water solution that 40 μ L concentration are 100mmol/L, final volume are 140 μ L.The starting reaction of ACE enzyme solution is added,
Reaction is terminated in 37 DEG C of constant temperature 1h, the HCI that 1mol/L is added.Then the ethyl acetate extraction of 1.2mL is added, 1mL is drawn in centrifugation
Ester layer dry 1h at 100 DEG C, is added 3mL distilled water, absorbance OD is measured at 228nm.
Blank control group is that nutrient water solution, absorbance OD are not added in above-mentioned reaction system1；
It is OD that positive controls, which are that addition inactivation ACE enzyme replaces original ACE enzyme absorbance in above-mentioned reaction system,0；
ACE inhibiting rate (%)=(OD1-OD)/(OD1-OD0)
The results are shown in Table 1:
Table 1
Test result shows: example 1 group, 2 groups of embodiment, 2 groups of comparative example have certain inhibitory effect to ACE.
Compared with 2 groups of comparative example, 2 groups of embodiment of ACE inhibiting rate is significantly improved, and illustrates to prepare solid mixture A's in 2 groups of embodiment
Method helps to be promoted final products nutrient to the inhibiting effect of ACE.
<egg white fermentation material evaluation experimental>
1, influence of the egg white pretreatment to amino acid composition and content in egg white fermentation material
The composition and content of amino acid in egg white fermentation material prepared by detection embodiment 2, comparative example 3, unit is mg/
The results are shown in Table 2 by 100mL:
Table 2
| Amino acid classes | Embodiment 2 | Comparative example 3 | Amino acid classes | Embodiment 2 | Comparative example 3 |
| Aspartic acid | 18.32 | 15.32 | Methionine * | 7.56 | 4.09 |
| Tyrosine | 7.45 | 5.34 | Isoleucine * | 12.56 | 10.21 |
| Serine | 18.43 | 17.32 | Leucine * | 13.23 | 8.97 |
| Glutamic acid | 25.76 | 23.98 | Threonine * | 11.23 | 10.32 |
| Proline | 2.12 | 0.32 | Phenylalanine * | 9.45 | 6.21 |
| Glycine | 10.23 | 8.34 | Lysine * | 28.21 | 20.14 |
| Alanine | 23.32 | 20.12 | Valine * | 3.24 | 0.12 |
| Cystine | 2.76 | 0.54 | Tryptophan * | 6.43 | 2.09 |
| Arginine | 7.43 | 4.23 | Histidine * | 4.87 | 1.28 |
Analysis of experimental results, the content of various amino acid is all remarkably higher than comparative example 3 in embodiment 2, illustrates embodiment 2
In egg white pretreatment, egg white can be promoted to decompose, promote various amino acid contents in egg white fermentation material.
2, influence of the partial acid digestion to polypeptide composition and content in egg white fermentation material
The composition and content of polypeptide in egg white fermentation material prepared by detection embodiment 2, comparative example 4, the results are shown in Table 3:
Table 3
Analysis of experimental results: most biologically active polypeptide molecular weight range is in 1000D-5000D, such as molecule
Measure 2500D-3000D polypeptide have very strong inoxidizability, such as molecular weight all have in the polypeptide of 3000D-5000D it is anti-
Fatigue effect；
Embodiment 2 improves content of peptides of the molecular weight between 1000D-5000D compared to comparative example 4, reduces point
Son amount is less than the content of peptides of 1000D, and improves the content of peptides that molecular weight is less than 5000D, illustrates to carry out freezing egg white
After micro porous filtration, only to upper object progress acidolysis processing is filtered, protein in egg white can be effectively prevented and excessively decompose, retaining has life
The active polypeptide segment of object, and the total content of biologically active polypeptide segment can be improved.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details and embodiment shown and described herein.
Claims (8)
1. the composite nutrient for preventing and treating hypertension, hyperlipemia disease, which is characterized in that be made including following parts by weight of component:
1.8 parts of hawthorn, 5 parts of sanchi flower, 5 parts of Camellia nitidissima, 2 parts of corn stigma, 8 parts of balsam pear, 6 parts of Black Box Tracing berry, cottonseed
0.2 part of oligosaccharide, 5 parts of egg white fermentation material, 2 parts of fermented ginseng powder, 0.2 part of wheat oligopeptide, 0.01 part of olive fruit powder, grape pip
0.05 part of extract, 0.1 part of black garlic powder, 0.01 part of theanine, 0.01 part of γ-aminobutyric acid, 0.02 part of L-arabinose.
2. the composite nutrient of prevention and treatment hypertension, hyperlipemia disease as described in claim 1, which is characterized in that the egg white fermentation
Object the preparation method comprises the following steps:
Step 1: egg white pre-processes: it is in mass ratio that 1:0.02:0.08:5 is mixed by egg white, mushroom taro powder, deionized water, Yu Wen
Degree is 10 DEG C, and mixing speed is that 30min is stirred under 600rpm, and sodium dihydrogen phosphate and jackfruit seed extract, Yu Wendu 10 is added
DEG C, mixing speed is to stir 3min under 100rpm, refrigerates 2 months at less than 10 DEG C, obtains refrigeration egg white；
Wherein, egg white, sodium dihydrogen phosphate, jackfruit seed extract mass ratio be 1:0.05:0.12；
Jackfruit seed extract the preparation method comprises the following steps: jackfruit seed is taken to be crushed to grain diameter less than 25 μm, light soy sauce and shell is added
Glycan is microwave treatment 5min under 500W in microwave power, and the ethyl alcohol that mass fraction is 95% is then added and extracts at 50 DEG C
3h filters to take filtrate and is concentrated to get jackfruit seed extract after volatilization removes ethyl alcohol；Wherein, jackfruit seed, light soy sauce, shell are poly-
Sugared, ethyl alcohol mass ratio is 0.12:0.004:0.002:1；
Step 2: enzymolysis processing: protease being added in the refrigeration egg white into step 1 and digests 4h at 15 DEG C, is then added multiple
Combined bacteria is fermented 50min at 15 DEG C, and being placed in magnetic field strength is to act on 15min under 1T, is then freezed 1 month, is obtained cold at -18 DEG C
Freeze egg white, after frozen egg removing summer-heat is frozen, carry out micro porous filtration, the aperture of micropore filtering film is 10 μm, retains and filters under upper object and filter
Object；Wherein, refrigerate egg white, protease, compound bacteria mass ratio be 1:0.02:0.04, compound bacteria by kluyveromyces marxianus,
Lactobacillus reuteri, staphylococcus xylosus are 1:1.2:1.5 composition in mass ratio；
Step 3: acidolysis is handled: lactic acid is added in object in the filter into step 2, is acidolysis 10d at 10 DEG C in temperature, carries out micropore
Filtering, the aperture of micropore filtering film are 10 μm, retain and filter lower object；Wherein, object in the filter in step 2, lactic acid mass ratio be 1:
0.3；
Step 4: merging object under the filter under the filter in step 2 in object and step 3, oligofructose and deionized water is then added,
It is to stir 10min under 300rpm in mixing speed, is drying to obtain egg white fermentation material；Wherein, object and step under the filter in step 2
The mass ratio of the quality summation, oligofructose, deionized water of object is 1:0.05:0.2 under filter in three.
3. the preparation method of the composite nutrient of prevention and treatment hypertension, hyperlipemia disease, feature exist as claimed in claim 1 or 2
In, comprising:
A, hawthorn, sanchi flower, Camellia nitidissima are mixed and are crushed, pectinase enzymatic hydrolysis 1h is then added, papain is then added,
5h is digested, after enzyme deactivation, is placed at 10 DEG C and refrigerates 5h, adds the water of 500 times of hawthorn quality, after stirring 20min, under 10MPa
8h is impregnated in pressurization, filters to take filtrate, by filtrate it is dry solid mixture A, wherein the quality of pectase is hawthorn quality
2%, the quality of papain is the 1.2% of hawthorn quality；
B, corn stigma, balsam pear, Black Box Tracing berry are mixed and is crushed, Lactobacillus saki is added, in 28 DEG C and infrared ray
After sterilizing, the ethyl alcohol that the mass fraction of 100 times of corn stigma quality is 95% is added in the irradiation lower 12h that ferments, stirring, be in temperature-
10 DEG C of freezing 12h filter to take filtrate then in 55 DEG C of heating water bath 5h, after filtrate recycling ethanol, are dried to obtain solid mixture
B；
C, by the solid mixture A in step a, the solid mixture B in step b, raffinose-oligosaccharide, egg white fermentation material, ginseng
Yeast powder, wheat oligopeptide, olive fruit powder, grape seed extract, black garlic powder, theanine, γ-aminobutyric acid, L-arabinose
It mixes up to nutrient.
4. the preparation method of the composite nutrient of prevention and treatment hypertension, hyperlipemia disease as claimed in claim 3, which is characterized in that step
The temperature of the 1h of pectinase enzymatic hydrolysis is 35 DEG C in rapid a, and the temperature of papain enzymolysis is 45 DEG C.
5. the preparation method of the composite nutrient of prevention and treatment hypertension, hyperlipemia disease as claimed in claim 3, which is characterized in that step
The intensity of infrared radiation is 100J/cm in rapid b2。
6. the preparation method of the composite nutrient of prevention and treatment hypertension, hyperlipemia disease as claimed in claim 3, which is characterized in that step
The inoculum concentration of Lactobacillus saki is the 0.6% of corn stigma quality in rapid b.
7. the preparation method of the composite nutrient of prevention and treatment hypertension, hyperlipemia disease as claimed in claim 3, which is characterized in that people
Ginseng yeast powder the preparation method comprises the following steps: take fresh ginseng, 20-30 DEG C of water temperature cleaning is slurried using beater, obtains ginseng pulp；To
In ginseng pulp plus water, amount of water are 40 times of volumes of ginseng pulp, cross colloid mill, obtain ginseng liquid；Ginseng liquid is heated to 40 DEG C, is added
Zymophyte, fermentation bacterium amount, which is behaved, joins 0.05 times of quality of liquid, and zymophyte is Bulgarian Lactobacillus and streptococcus thermophilus 1:1 mass
Than ferment 10h, decomposes polysaccharose substance, and ginsenoside is precipitated, obtains fermented ginseng stoste；It is freeze-dried to obtain fermented ginseng powder.
8. such as application of the nutrient as claimed in any one of claims 1 to 6 in the product for preparing preventing hypertension hyperlipidemia.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN113142300A (en) * | 2021-05-10 | 2021-07-23 | 锡林郭勒职业学院 | Method for preparing horse milk product with ACE (angiotensin converting enzyme) inhibitory activity by combining yeast fermentation and enzymolysis |
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