CN109717110A - A kind of no squama giant clam seed industrial production method - Google Patents
A kind of no squama giant clam seed industrial production method Download PDFInfo
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- CN109717110A CN109717110A CN201910124425.6A CN201910124425A CN109717110A CN 109717110 A CN109717110 A CN 109717110A CN 201910124425 A CN201910124425 A CN 201910124425A CN 109717110 A CN109717110 A CN 109717110A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
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Abstract
The invention discloses a kind of no squama giant clam seed industrial production methods.It discharged naturally including parent ecology accelerating, gamete, fertilization and hatching, larvae cultivation, feed zooxanthellae, settlement and metamorphosis, juvenile mollusk culture and young shellfish and be bred as.The large-scale cultivation in China goes out rare, medium-and-large-sized no squama giant clam large size seedling seed to the present invention for the first time, has filled up domestic blank；Batch production fry production process has reached domestic advanced, international level of taking the lead in race, and provides reliable technical guarantee for the large and medium-sized giant clam resource recovery in the South Sea.The present invention provides reliable technical guarantee for no squama giant clam Seedling production, provides possibility for the high-end giant clam product supply in seawater Shui nationality market, provides approach for the large and medium-sized giant clam resource recovery in China South Sea.
Description
Technical field:
The invention belongs to shellfishes in Marine agriculture to breed cultural technique field, and in particular to a kind of no squama giant clam seed factory
Change production method.
Background technique:
In China, the giant clam that giant clam belongs to has 6 kinds, respectively Ku Shi giant clam, without squama giant clam, squama giant clam, Tridacna (chamestrachea) maxima (R ding), Nova Che
Giant clam, sarranine giant clam.Wherein, Ku Shi giant clam is the maximum giant clam of individual, also known as big giant clam, the long reachable 2m of maximum shell, fresh weight
500kg；No squama giant clam is medium-and-large-sized giant clam, also known as coconut shellfish, and maximum shell is long up to 60cm, fresh weight 50kg；Other giant clams are
Middle-size and small-size giant clam, shell length≤40cm, fresh weight≤10kg.In China's South China Sea, at present almost without living body Ku Shi giant clam, no squama
Giant clam also tends to be in imminent danger, although there are also certain biomass for other species giant clams, quantity is also few, and giant clam resource recovery is compeled in eyebrow
Eyelash.
Chinese Academy of Science Nanhai Ocean Research Institute realizes China's giant clam artificial seed's raising technology for the first time, has filled up the country
Blank, but the giant clam bred out is middle-size and small-size giant clam, including squama giant clam, Tridacna (chamestrachea) maxima (R ding), Nova giant clam and sarranine giant clam, at present still
Have no the related technology reports (Zhang Yuehuan etc., 2016,2017) of no squama giant clam breeding.So, so rare big-and-middle in China
Can type breed success without squama giant clam, and whether seed can become critical issue urgently to be resolved with the factorial production.In order to solve
The card neck subproblem, the present invention provides a kind of no squama giant clam seed industrial production method and applications, for no squama giant clam seed
Production provides reliable technical guarantee, provides possibility for the high-end giant clam product supply in seawater Shui nationality market, is China south
The large and medium-sized giant clam resource recovery in sea provides approach.
Summary of the invention:
The purpose of the invention is to overcome defect in the prior art, a kind of no squama giant clam seed the factorial production is provided
Method.The present invention provides reliably for China's large and medium-sized no squama giant clam artificial breeding cultivation in imminent danger, enhancement releasing, resource recovery
Technical guarantee.
It is of the invention without squama giant clam seed industrial production method, comprising the following steps:
A, parent ecology accelerating: choosing wild no squama giant clam and put in a suitable place to breed in culturing jar as parent, is filled using miniflow water+micro-
Gas raising, daily streamflow are 2-3 times of culturing jar total volume；Put top shell in a suitable place to breed in culturing jar simultaneously；
B, gamete discharges naturally: in the annual 5-6 month without squama giant clam breeding period, discharging smart ovum naturally to it, is collected separately
Smart ovum；
C, fertilization and hatching: using allogamous principle, according to essence: ratio of the ovum quantity than=20-30:1 is inseminated,
Triploidy rate controls within 100/mL, and fertilized eggs liquid is poured into the hatching cylinder for filling fresh seawater, density control by after fertilization
System is in 30-40/mL, micro- inflation+stirring hatching, development of fertilized ova to D-shaped larva；
D, larvae cultivation: D-shaped larva is collected using bolting silk net, is placed in the culturing jar for filling fresh seawater, adopts later
Larvae cultivation is carried out with micro- inflation, density is 3-4/mL；During this period, water, not bait throwing in are not changed；
E, it feeds zooxanthellae: when 5 age in days of larvae cultivation, starting to feed zooxanthellae, feeding density is 30-50/mL, often
It feeds 1 time, it is ensured that has 2-3 zooxanthellae in 90% or more larva glandula digestive；During this period, water is not changed, is only inflated；
F, settlement and metamorphosis: when larvae development enters the settlement and metamorphosis phase, during this period, not changing water, only inflates, and larva completes to become
State；
G, juvenile mollusk culture: when larva completes abnormal formation juvenile mollusk；Using not changing water+micro- airing form culture juvenile mollusk, juvenile mollusk
Develop into young shellfish；
H, children Bei Yucheng: the seawater in culturing jar is emptied, and collects young shellfish, is placed in later and is paved with the young shellfish of corallite and supports
It grows in cylinder, according to 20000-30000/m2Density is placed, after putting young shellfish well, non-aerating, not stream treatment 5-6h, young Bei Gu
On corallite, it is micro- later inflate+not change Aquaponic children shellfish and grow to 3-4mm, by corallite together with the young shellfish adhered to above
It is placed in semi-enclosed circulation together, density domination is in 3000-4000/m2, taken a picture using miniflow water+natural light
Binding pattern cultivates children Bei Zhi great specification children shellfish.
The breeding density of the wild no squama giant clam is preferably 20kg/m2, the breeding density of top shell is preferably
0.2kg/m2。
It is preferred that parent's shell of selection is grown in 40cm or more in the step a parent ecology accelerating；During ecological accelerating,
Seawater salinity 30-35ppt, 25-30 DEG C of temperature, pH 7.9-8.3, seawater holding total nitrogen≤0.5mg/L, total phosphorus≤0.1mg/L,
Light source is natural light, and intensity control is in 0-15000Lux.
It is described in the annual 5-6 month without squama giant clam breeding period, discharge smart ovum naturally to it, smart ovum, which is collected separately, is specially
The annual 5-6 month is no squama giant clam breeding period, during this period, every 7 days fall parent Bei Yici, discharged naturally to it essence ovum；Usually exist
1-3 point in afternoon before and after the lunar calendar junior one, 15 discharges smart ovum, and parent discharges sperm first, can about continue 30-40min, it
After be spaced 10-20min, start discharge ovum, continue 60-90min, smart ovum is collected separately.
It is preferred that 27.8-28.6 DEG C of ocean temperature during the step d larvae cultivation, salinity 32ppt, pH 7.9-
8.3, light source is natural light, and intensity control is in 0-6000Lux.
It is preferred that the step e feed zooxanthellae during 28.2-29.0 DEG C of ocean temperature, salinity 32ppt, pH 8.0-
8.3；The zooxanthellae fed includes A1 type zooxanthellae, C1 type zooxanthellae and E type zooxanthellae, the A1 type zooxanthellae,
The quantity ratio of C1 type zooxanthellae and E type zooxanthellae is 1:1:1.
It is preferred that 28.3-29.2 DEG C of ocean temperature during the step f settlement and metamorphosis, salinity 32ppt, pH 8.0-
8.3, light source is natural light, and intensity control is in 0-9000Lux.
It is preferred that the light source during the step g juvenile mollusk culture is natural light, intensity control is in 0-15000Lux.
It is preferred that 27-30 DEG C of ocean temperature during the step h children shellfish incubation, salinity 30-35ppt, pH 7.9-
8.3, sea water advanced 30-60cm.
It is preferred that the semi-enclosed circulation includes the reservoir for circuiting sequentially connection by circulating line, follows
Ring pump, temperature controlling instruments, open type glass cylinder runway, physical filtering equipment and protein separator；The open type glass cylinder is run
Road is the cuboid glass jar of upper end opening.
A1, C1, E type zooxanthellae of the invention is A1, C1, E the type zooxanthellae manually cultivated, and is referred to according to patent of invention
The zooxanthellae from giant clam outer embrane that (publication number: 107574123A) technical method is cultivated.
The present invention and documents 1 (a kind of artificial breeding method of giant clam of Zhang Yuehuan, Xiao Shu, Yu Ziniu, application number:
201610454619.9) different, have following main difference: (1) research object is different: 1 research object of documents is
Middle-size and small-size giant clam (squama giant clam, Tridacna (chamestrachea) maxima (R ding), sarranine giant clam), and the present invention is large and medium-sized giant clam (no squama giant clam)；(2) technological means
It is different: documents 1 be by parent's accelerating, artificial induced spawning, fertilization and hatching, larvae cultivation, zooxanthellae implantation, settlement and metamorphosis,
Juvenile mollusk cultivate etc. sport technique segments, and the present invention be discharged naturally by parent ecology accelerating, gamete, fertilization and hatching, larvae cultivation,
Feed sport technique segments, the two such as zooxanthellae, settlement and metamorphosis, juvenile mollusk culture, young shellfish incubation has significant technology in these links
Difference, in short, documents 1 and the present invention are the technical solution that sets according to every kind of giant clam biological property, thus, it deposits
In significant difference；(3) effect is entirely different: it is 1% or so that documents 1, which produce larval metamorphosis rate, and larva of the present invention becomes
State rate >=30%；Documents 1 were only cultivated to shell a length of 1-2mm children shellfish stage, and the present invention turns out the big rule of shell length >=3cm
Lattice children shellfish；Documents 1 are experimental fry production process, and the present invention is the factorial production technology, and the two is in quantity size
On there is significance differences.It can be seen that the present invention is the innovative patent of optimization compared with documents 1, with height
It is innovative.
Detailed description of the invention:
Fig. 1 is the structural schematic diagram of semi-enclosed circulation of the invention；Wherein, 1, reservoir；2, circulating line；
3, circulating pump；4, temperature controlling instruments；5, open type glass cylinder runway；6, physical filtering equipment；7, protein separator.
Fig. 2 is no squama giant clam artificial breeding schematic diagram.A, parent；B, development of fertilized ova to blastula stage；C, mid-term face children
Worm；D, sufficient veliger；E collects the young shellfish of long 1mm of shell or so；F, the long 1-2mm children shellfish of shell；G, the long 3-4mm children of shell
Shellfish；H, the long 3-5cm children shellfish of shell.
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
Embodiment 1:
Semi-enclosed circulation as shown in Figure 1, including by circulating line 2 circuit sequentially connection reservoir 1,
Circulating pump 3, temperature controlling instruments 4, open type glass cylinder runway 5, physical filtering equipment 6 and protein separator 7；Open type glass cylinder is run
Road is the cuboid glass jar (length × width × height=20m × 2.0m × 80cm) of upper end opening.
The workflow of semi-enclosed circulation of the invention is as follows: the seawater in reservoir 1 is pumped by circulating pump 3
Enter circulating line 2, enters open type glass cylinder runway 5 after temperature controlling instruments 4 adjusts temperature, flow through open type glass cylinder runway 5
Seawater flow back to reservoir 1 after physical filtering equipment 6 and protein separator 7 again, recirculated water operation always later.
The present embodiment without squama giant clam seed industrial production method, comprising the following steps:
A, parent ecology accelerating: on July 25th, 2017, commission China South Sea fisherman fish back 10 wild no squama giant clams
As parent, the long 40-50cm of shell, fresh weight 10-20kg/；According to 20kg/m2It puts in a suitable place to breed in Chinese Academy of Science Nanhai Ocean Research Institute
It in the culturing jar at Hainan Tropical marine biological laboratory station, is raised using miniflow water+micro- inflation, daily streamflow is that culturing jar is overall
Long-pending 2 times；Meanwhile according to 0.2kg/m2Top shell is placed, for clearing up culturing jar and parent's shell table filiform class；It is protected in seawater
Hold total nitrogen≤0.5mg/L, total phosphorus≤0.1mg/L；During ecological accelerating, seawater salinity 30-35ppt, 25-30 DEG C of temperature, pH
7.9-8.3, light source are natural light, light intensity 0-15000Lux.
B, gamete discharges naturally: on May 15th, 2018 morning 11:00 point, by 8 without squama giant clam parent transfer to 1 contain
Have in the culturing jar of fresh seawater, afternoon, 1:30 started to discharge smart ovum；It was found that 2 individual discharge sperms, are individually placed at
In two aquariums, 40min about continue for, be spaced 10min after discharging sperm to it, transfer them to new culturing jar
In continue discharge ovum, the discharge ovum time be 60min, be achieved in that monomer essence ovum；
C, fertilization and hatching: after gamete has successfully been obtained, sperm motility, two individual sperms 90% are checked under the microscope
The above locomitivity is relatively strong (spasmodic motion), belongs to superior in quality sperm；Find that ovum is relatively round under microscope, ovum diameter is 100 μ
M, nutriment is abundant in ovum, blackyellow is presented, egg membrane is smooth, belongs to high quality quality ovum；Using superior in quality sperm and
High quality quality ovum fertilization, using allogamous principle, according to essence: ovum quantity ratio=20:1 fertilization, triploidy rate control
Fertilized eggs liquid is poured into the hatching cylinder for filling fresh seawater after the 30min that is fertilized, density domination is 30 within 100/mL by system
A/mL, micro- inflation+stirring hatching are passed through under the conditions of 27.5-28.0 DEG C of ocean temperature, salinity 30ppt, pH 7.9-8.2
30h hatches development of fertilized ova to D-shaped larva；After testing, the rate of fertilization of two groups of fertilized eggs is close to 100%, hatching rate >=
90%；
D, larvae cultivation: D-shaped larva (125 μ of shell length × shell height=150 μ m is collected using the bolting silk net that mesh is 70 μm
M), it is placed in 2 0.25m for filling fresh seawater later3Culturing jar in, using micro- inflation carry out larvae cultivation, density
For 4/mL, amounts to and obtain larva 2,000,000；During this period, water, not bait throwing in are not changed；27.8-28.6 DEG C of ocean temperature, salinity
32ppt, pH 7.9-8.3, light source are natural light, light intensity 0-6000Lux；
E, it feeds zooxanthellae: when 5 age in days of larvae cultivation (shell length reaches 190 μm), starting to feed A1, C1, the E manually cultivated
Type zooxanthellae, 3 kinds of zooxanthellae quantity ratios are 1:1:1, and feeding density is 30/mL, feed 1 time daily, continuously feed 5d,
There is 2-3 zooxanthellae in 90% or more larva glandula digestive；During this period, water is not changed, is only inflated；28.2-29.0 DEG C of ocean temperature,
Salinity 32ppt, pH 8.0-8.3；
F, settlement and metamorphosis: reaching 210 μm when embryophore is long, and larva face disappears, and is switched to bottom by swimming and is dwelt life, by foot
It creeps, the cilium ring-type gill, secondary shell occurs, zooxanthellae quantity >=20 in glandula digestive have been built up early stage zooxanthellae pipeline system
System, that is, complete metamorphosis, and whole process needs 5-7d；During this period, water is not changed, is only inflated；28.3-29.2 DEG C of ocean temperature, salt
32ppt, pH 8.0-8.3 are spent, light source is natural light, light intensity 0-9000Lux；
G, juvenile mollusk culture: after larva completes abnormal, shell is up to 230 μm, that is, forms juvenile mollusk；Juvenile mollusk shell at this time is saturating
It is bright, it is creeped with foot, nutrition needed for individual is entirely from zooxanthellae；Using water+micro- airing form culture juvenile mollusk is not changed, obtain altogether
Juvenile mollusk 670,000, juvenile mollusk 40d is cultivated, juvenile mollusk develops into young shellfish, shell length >=1mm, and young Becquerel at this time can secrete foot to stand
Silk, anchors at cylinder bottom for its own；Young shellfish 410,000 of the long 1-2mm of shell are obtained altogether；Light source is natural light, light intensity 0-
15000Lux；
H, children Bei Yucheng: culturing jar seawater is emptied, and is collected young shellfish, is placed in the young shellfish culturing jar for being paved with corallite later
In, according to 20000/m2Density is placed, non-aerating, stream treatment 5h, young shellfish do not anchor at corallite after putting young shellfish well
On, micro- later inflate+not change Aquaponic 30d, young shellfish grows to 3-4mm；By its corallite together with the young Bei Yitong adhered to above
It is placed in the open type glass cylinder runway of semi-enclosed circulation, density domination is in 3000/m2, ocean temperature 27-
30 DEG C, salinity 30-32ppt, pH7.9-8.3, sea water advanced 30cm combine mode, every sky and water using miniflow water+natural lighting
Flow control cultivated by 2 months at 5 times of open type glass cylinder runway volume, obtains the big specification children shellfish of shell length >=3cm
360000 (Fig. 2).
Technological means through this embodiment, we successfully breed out without squama giant clam seed.It cultivated, obtains by 2 months
670,000 juvenile mollusks by cultivation in 3 months obtain the young shellfish of 410,000 3-4mm；By cultivation in 4 months, young shellfish 360,000
It is a.
Embodiment 2:
The present embodiment without squama giant clam seed industrial production method, comprising the following steps:
A, parent ecology accelerating: on September 16th, 2017, commission China South Sea fisherman fish back 6 wild no squama giant clams
As parent, the long 40-50cm of shell, fresh weight 15-30kg/；According to 20kg/m2It puts in a suitable place to breed in Chinese Academy of Science Nanhai Ocean Research Institute
It in the culturing jar at Hainan Tropical marine biological laboratory station, is raised using miniflow water+micro- inflation, daily streamflow is that culturing jar is overall
Long-pending 3 times；Meanwhile according to 0.2kg/m2Top shell is placed, for clearing up culturing jar and parent's shell table filiform class；It is protected in seawater
Hold total nitrogen≤0.5mg/L, total phosphorus≤0.1mg/L；During ecological accelerating, seawater salinity 30-33ppt, 26-30 DEG C of temperature,
PH7.9-8.3, light source are natural light, light intensity 0-15000Lux.
B, gamete discharges naturally: on May 9th, 2018 morning 11:30 point, 6 are filled without squama giant clam parent transfer to 1
In the culturing jar of fresh seawater, afternoon, 2:00 started to discharge smart ovum；It was found that 3 individual discharge sperms, are individually placed at two
In a aquarium, 30min about continue for, be spaced 20min after discharging sperm to it, transfer them in new culturing jar
Continue to discharge ovum, the discharge ovum time is 90min, has been achieved in that monomer essence ovum；
C, fertilization and hatching: after gamete has successfully been obtained, sperm motility, two individual sperms 90% are checked under the microscope
The above locomitivity is relatively strong (spasmodic motion), belongs to superior in quality sperm；Find that ovum is relatively round under microscope, ovum diameter is 100 μ
M, nutriment is abundant in ovum, blackyellow is presented, egg membrane is smooth, belongs to high quality quality ovum；Using superior in quality sperm and
High quality quality ovum fertilization, using allogamous principle, according to essence: ovum quantity ratio=20:1 fertilization, triploidy rate control
Fertilized eggs liquid is poured into the hatching cylinder for filling fresh seawater after the 30min that is fertilized, density domination is 40 within 100/mL by system
A/mL, micro- inflation+stirring hatching are passed through under the conditions of 27.6-28.2 DEG C of ocean temperature, salinity 30ppt, pH 7.9-8.3
30h hatches development of fertilized ova to D-shaped larva；After testing, the rate of fertilization of two groups of fertilized eggs is close to 100%, hatching rate >=
90%；
D, larvae cultivation: D-shaped larva (125 μ of shell length × shell height=150 μ m is collected using the bolting silk net that mesh is 70 μm
M), it is placed in 2 0.5m for filling fresh seawater later3Culturing jar in, larvae cultivation is carried out using micro- inflation, density is
2/mL, amounts to and obtain larva 2,000,000；During this period, water, not bait throwing in are not changed；27.6-28.8 DEG C of ocean temperature, salinity
32ppt, pH 7.9-8.3, light source are natural light, light intensity 0-6000Lux；
E, it feeds zooxanthellae: when 5 age in days of larvae cultivation (shell length reaches 190 μm), starting to feed A1, C1, the E manually cultivated
Type zooxanthellae, 3 kinds of zooxanthellae quantity ratios are 1:1:1, and feeding density is 50/mL, feed 1 time daily, continuously feed 6d,
There is 2-3 zooxanthellae in 90% or more larva glandula digestive；During this period, water is not changed, is only inflated；28.3-29.1 DEG C of ocean temperature,
Salinity 32ppt, pH 8.0-8.3；
F, settlement and metamorphosis: reaching 210 μm when embryophore is long, and larva face disappears, and is switched to bottom by swimming and is dwelt life, by foot
It creeps, the cilium ring-type gill, secondary shell occurs, zooxanthellae quantity >=20 in glandula digestive have been built up early stage zooxanthellae pipeline system
System, that is, complete metamorphosis, and whole process needs 5-7d；During this period, water is not changed, is only inflated；28.4-29.1 DEG C of ocean temperature, salt
32ppt, pH 8.0-8.3 are spent, light source is natural light, light intensity 0-9000Lux；
G, juvenile mollusk culture: after larva completes abnormal, shell is up to 230 μm, that is, forms juvenile mollusk；Juvenile mollusk shell at this time is saturating
It is bright, it is creeped with foot, nutrition needed for individual is entirely from zooxanthellae；Using water+micro- airing form culture juvenile mollusk is not changed, obtain altogether
Juvenile mollusk 1,200,000, juvenile mollusk 50d is cultivated, juvenile mollusk develops into young shellfish, shell length >=1mm, and young Becquerel at this time can be secreted to stand
Its own is anchored at cylinder bottom by byssus；Young shellfish 1,080,000 of the long 1-2mm of shell are obtained altogether；Light source is natural light, light intensity 0-
15000Lux；
H, children Bei Yucheng: culturing jar seawater is emptied, and is collected young shellfish, is placed in the young shellfish culturing jar for being paved with corallite later
In, according to 30000/m2Density is placed, non-aerating, stream treatment 6h, young shellfish do not anchor at corallite after putting young shellfish well
On, micro- later inflate+not change Aquaponic 30d, young shellfish grows to 3-4mm；By its corallite together with the young Bei Yitong adhered to above
It is placed in the open type glass cylinder runway of semi-enclosed circulation (with embodiment 1), density domination is in 4000/m2,
27-30 DEG C of ocean temperature, salinity 30-32ppt, pH7.9-8.3, sea water advanced 60cm is combined using miniflow water+natural lighting
Mode, daily water flow control cultivated by 2 months at 10 times of open type glass cylinder runway volume, obtain shell length >=3cm
Big specification children shellfish 83.6 ten thousand (Fig. 2).
Technological means through this embodiment, we successfully breed out without squama giant clam seed.It cultivated, obtains by 2 months
1,200,000 juvenile mollusks by cultivation in 3 months obtain the young shellfish of 900,000 3-4mm；By cultivation in 4 months, young shellfish
83.6 ten thousand.
Main innovation point of the invention is that go out rare, medium-and-large-sized no squama giant clam big for large-scale cultivation in China for the first time
Size seedling seed has filled up domestic blank；Batch production fry production process has reached domestic advanced, international level of taking the lead in race, and is the South Sea
Large and medium-sized giant clam resource recovery provides reliable technical guarantee.
Claims (9)
1. a kind of no squama giant clam seed industrial production method, which comprises the following steps:
A, it parent ecology accelerating: chooses wild no squama giant clam and is put in a suitable place to breed in culturing jar as parent, raised using miniflow water+micro- inflation
It supports, daily streamflow is 2-3 times of culturing jar total volume；Put top shell in a suitable place to breed in culturing jar simultaneously；
B, gamete discharges naturally: in the annual 5-6 month without squama giant clam breeding period, discharging smart ovum naturally to it, smart ovum is collected separately；
C, fertilization and hatching: using allogamous principle, according to essence: ratio of the ovum quantity than=20-30:1 is inseminated, and is fertilized
For ovum density domination within 100/mL, fertilized eggs liquid is poured into the hatching cylinder for filling fresh seawater by after fertilization, and density domination exists
30-40/mL, micro- inflation+stirring hatching, development of fertilized ova to D-shaped larva；
D, larvae cultivation: D-shaped larva is collected using bolting silk net, is placed in the culturing jar for filling fresh seawater later, use is micro-
Inflation carries out larvae cultivation, and density is 3-4/mL；During this period, water, not bait throwing in are not changed；
E, it feeds zooxanthellae: when 5 age in days of larvae cultivation, starting to feed zooxanthellae, feeding density is 30-50/mL, is thrown daily
It feeds 1 time, it is ensured that have 2-3 zooxanthellae in 90% or more larva glandula digestive；During this period, water is not changed, is only inflated；
F, settlement and metamorphosis: when larvae development enters the settlement and metamorphosis phase, during this period, not changing water, only inflates, and larva is completed abnormal；
G, juvenile mollusk culture: when larva completes abnormal formation juvenile mollusk；Using water+micro- airing form culture juvenile mollusk is not changed, juvenile mollusk is developed
Cheng Youbei；
H, children Bei Yucheng: the seawater in culturing jar is emptied, and is collected young shellfish, is placed in the young shellfish culturing jar for being paved with corallite later
In, according to 20000-30000/m2Density is placed, non-aerating, stream treatment 5-6h, young shellfish do not anchor at after putting young shellfish well
It is micro- later inflate+not change Aquaponic children shellfish and grow to 3-4mm on corallite, by corallite together with the young Bei Yitong adhered to above
It is placed in semi-enclosed circulation, density domination is in 3000-4000/m2, combined using miniflow water+natural lighting
Mode cultivates children Bei Zhi great specification children shellfish.
2. no squama giant clam seed industrial production method according to claim 1, which is characterized in that the wild no squama
The breeding density of giant clam is 20kg/m2, the breeding density of top shell is 0.2kg/m2。
3. no squama giant clam seed industrial production method according to claim 1 or 2, which is characterized in that the step a
In parent ecology accelerating, parent's shell of selection is grown in 40cm or more；During ecological accelerating, seawater salinity 30-35ppt, temperature 25-
30 DEG C, pH 7.9-8.3, seawater keeps total nitrogen≤0.5mg/L, and total phosphorus≤0.1mg/L, light source is natural light, and intensity control is in 0-
15000Lux。
4. no squama giant clam seed industrial production method according to claim 1 or 2, which is characterized in that the step d
27.8-28.6 DEG C of ocean temperature during larvae cultivation, salinity 32ppt, pH 7.9-8.3, light source is natural light, intensity control
In 0-6000Lux.
5. no squama giant clam seed industrial production method according to claim 1 or 2, which is characterized in that the step e
Feed 28.2-29.0 DEG C of ocean temperature during zooxanthellae, salinity 32ppt, pH 8.0-8.3；The zooxanthellae packet fed
Include A1 type zooxanthellae, C1 type zooxanthellae and E type zooxanthellae, the number of the A1 type zooxanthellae, C1 type zooxanthellae and E type zooxanthellae
Amount is than being 1:1:1.
6. no squama giant clam seed industrial production method according to claim 1 or 2, which is characterized in that the step f
28.3-29.2 DEG C of ocean temperature during settlement and metamorphosis, salinity 32ppt, pH 8.0-8.3, light source is natural light, intensity control
In 0-9000Lux.
7. no squama giant clam seed industrial production method according to claim 1 or 2, which is characterized in that the step g
Light source during juvenile mollusk culture is natural light, and intensity control is in 0-15000Lux.
8. no squama giant clam seed industrial production method according to claim 1 or 2, which is characterized in that the step h
27-30 DEG C of ocean temperature during young shellfish incubation, salinity 30-35ppt, pH 7.9-8.3, sea water advanced 30-60cm.
9. no squama giant clam seed industrial production method according to claim 1 or 2, which is characterized in that half envelope
Closed circle water system includes the reservoir that connection is circuited sequentially by circulating line, circulating pump, temperature controlling instruments, open type glass
Cylinder runway, physical filtering equipment and protein separator；The open type glass cylinder runway is the cuboid glass of upper end opening
Cylinder.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114304011A (en) * | 2021-11-29 | 2022-04-12 | 中国科学院南海海洋研究所 | Method for cultivating young mollusks of fagopyrum |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100009036A (en) * | 2008-07-17 | 2010-01-27 | 대한민국(관리부서:국립수산과학원) | Fish breeding farm for preventing pleuronectiformes from hypermelanosis |
| CN103798166A (en) * | 2014-01-24 | 2014-05-21 | 中国科学院南海海洋研究所 | Method for large-scale indoor artificial breeding of Hong Kong oysters in coastal region of South China |
| CN105918185A (en) * | 2016-06-21 | 2016-09-07 | 李育培 | Method for artificial breeding of tridacnidae |
| CN105941233A (en) * | 2016-06-20 | 2016-09-21 | 中国科学院南海海洋研究所 | Chemical method for effectively avoiding self-fertilization of tridacna |
| CN106069922A (en) * | 2016-06-20 | 2016-11-09 | 中国科学院南海海洋研究所 | A kind of method improving Concha Tridacna squamosa larval metamorphosis rate |
| CN106106272A (en) * | 2016-06-20 | 2016-11-16 | 中国科学院南海海洋研究所 | A kind of Che oyster artificial breeding method |
| CN106165659A (en) * | 2016-06-20 | 2016-11-30 | 中国科学院南海海洋研究所 | A kind of Concha Tridacna squamosa artificial breeding method |
| CN106857344A (en) * | 2017-03-22 | 2017-06-20 | 中国科学院南海海洋研究所 | A kind of method for improving giant clam children's shellfish Transitional culture efficiency |
-
2019
- 2019-02-19 CN CN201910124425.6A patent/CN109717110A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20100009036A (en) * | 2008-07-17 | 2010-01-27 | 대한민국(관리부서:국립수산과학원) | Fish breeding farm for preventing pleuronectiformes from hypermelanosis |
| CN103798166A (en) * | 2014-01-24 | 2014-05-21 | 中国科学院南海海洋研究所 | Method for large-scale indoor artificial breeding of Hong Kong oysters in coastal region of South China |
| CN105941233A (en) * | 2016-06-20 | 2016-09-21 | 中国科学院南海海洋研究所 | Chemical method for effectively avoiding self-fertilization of tridacna |
| CN106069922A (en) * | 2016-06-20 | 2016-11-09 | 中国科学院南海海洋研究所 | A kind of method improving Concha Tridacna squamosa larval metamorphosis rate |
| CN106106272A (en) * | 2016-06-20 | 2016-11-16 | 中国科学院南海海洋研究所 | A kind of Che oyster artificial breeding method |
| CN106165659A (en) * | 2016-06-20 | 2016-11-30 | 中国科学院南海海洋研究所 | A kind of Concha Tridacna squamosa artificial breeding method |
| CN105918185A (en) * | 2016-06-21 | 2016-09-07 | 李育培 | Method for artificial breeding of tridacnidae |
| CN106857344A (en) * | 2017-03-22 | 2017-06-20 | 中国科学院南海海洋研究所 | A kind of method for improving giant clam children's shellfish Transitional culture efficiency |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114304011A (en) * | 2021-11-29 | 2022-04-12 | 中国科学院南海海洋研究所 | Method for cultivating young mollusks of fagopyrum |
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