CN109700018A - A kind of tree peony ferment and its production method improving cleaning blood free radicals - Google Patents
A kind of tree peony ferment and its production method improving cleaning blood free radicals Download PDFInfo
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Abstract
The invention discloses a kind of tree peony ferment and its production method for improving cleaning blood free radicals, using peony, chamomile, carrot, pineapple, pawpaw, tomato, orange peel, wax gourd, mulberry leaf, pyracantha fortuneana fruit, Semen Coicis, oat, mung bean, grape pip, lemon as raw material, bifidobacterium lactis, aspergillus oryzae, Lactobacillus rhamnosus, lactobacillus plantarum, Lactobacillus delbrueckii, Hansenula yeast and kluyveromyces marxianus are strain, the loss of effective component in raw material can be effectively reduced, the nutritional ingredient for retaining each raw material to greatest extent enriches the nutrition of peony.Ferment provided by the invention is unique in taste, and has no toxic side effect, and will not generate harm to the health of human body, long-term consumption can remove free radical, have anti-oxidant, and strength supplement is adjusted, nutrient balance in antagonism human body, internal heavy metal and toxin are excluded, oxygenation waste discharge is adjusted immune, improve metabolism, enhance cell viability, promote blood circulation, eliminates prevention disease, health care prolongs life.
Description
Technical field
The present invention relates to tree peony ferment field more particularly to a kind of tree peony ferment for improving cleaning blood free radicals and its lifes
Production method.
Background technique
Oxygen radical is a kind of harmful substance, referred to as " killers of good health and a long life ", it can cause biofilm system to damage
And Cellular Oxidation phosphorylation obstacle, it even causes people in fatal position to kill healthy cell, is human body diseases, aging and death
Direct participant, the health and long-lived harm to human body is very large.
Activity in vivo oxygen radical has the function of certain, such as immune and signal transduction process.But excessive active oxygen is certainly
Destructiveness is just had by base, leads to the damage of human normal cell and tissue, so as to cause a variety of diseases, referred to as ten thousand diseases
Source, such as color spot, trick is ice-cold, heart disease, senile dementia, Parkinson's disease and tumour.In addition, the sunlight spoke in external environment
Penetrate, air pollution, smoking, pesticide etc. can all make human body generate more active oxygen radicals, make nucleic acid mutation, this is mankind aging
With the root of illness.
For the intracorporal free radical of removing machine, the damage to body is reduced, restores body health, we are using oxidation resistant
Method tackles oxygen radical.Current existing anti-oxidation medicine is mainly enzyme system (antioxidase) drug, including super oxygen
Object mutase, glutathione peroxidase, catalase and monoamine oxidase inhibitors etc.；Drug is anti-oxidant mainly have with
Lower disadvantage: (1) exogenous supplement, human body itself function fail really to repair, and are used for a long time, and are easy to upset intrinsic metabolism, parent
It is low with power；(2) molecular weight is too big, is difficult smoothly to penetrate cell membrane, if oral, loses activity vulnerable to protease hydrolytic；(3)
There is adverse reaction, such as: Nausea and vomiting, headache and fash also there are allergy sufferers.
Summary of the invention
The object of the present invention is to provide a kind of tree peony ferment and its production method for improving cleaning blood free radicals, Neng Gouqing
Except free radical, have anti-oxidant, strength supplement is adjusted, nutrient balance in antagonism human body.
In order to solve the above technical problems, the technical solution adopted by the present invention are as follows:
It is a kind of improve cleaning blood free radicals tree peony ferment, including peony, chamomile, carrot, pineapple, pawpaw,
Tomato, orange peel, wax gourd, mulberry leaf, pyracantha fortuneana fruit, Semen Coicis, oat, mung bean, grape pip, lemon are raw material, and mixed bacteria is added
It ferments；
The mixed bacteria include bifidobacterium lactis, aspergillus oryzae, Lactobacillus rhamnosus, lactobacillus plantarum, Lactobacillus delbrueckii,
Hansenula yeast and kluyveromyces marxianus.
Preferably, the bifidobacterium lactis, aspergillus oryzae, the Lactobacillus rhamnosus, the lactobacillus plantarum, described
The mass ratio of Lactobacillus delbrueckii, the Hansenula yeast and the kluyveromyces marxianus is (0.5~1): (1.5~2): (0.3
~0.5): (1~2): (0.5~1): (2~3): (0.8~1.2).
Preferably, the peony, the chamomile, the carrot, the pineapple, the pawpaw, the tomato, institute
State orange peel, the wax gourd, the mulberry leaf, the pyracantha fortuneana fruit, the Semen Coicis, the oat, the mung bean, the grape pip
Mass ratio with the lemon is (4~5): (0.5~0.8): (1~2): (1~2): (1~1.5): (1~2): (0.5~1):
(0.4~0.7): (0.3~0.5): (0.5~1): (1~2): (1~2): (0.8~1.2): (0.5~0.6): (0.2~
0.4)。
The present invention also provides a kind of production methods of tree peony ferment for improving cleaning blood free radicals, including following step
It is rapid:
1) raw material selection is cleaned: peony, carrot, pineapple, pawpaw, tomato, wax gourd, pyracantha fortuneana fruit, lemon are by selecting
Water-bath cleaning machine is entered by conveyer belt afterwards, ejected wash water residual chlorine concentration is 60~80ppm, 30~60s of scavenging period；
2) pretreatment of raw material: by the peony of step 1), carrot, pineapple, pawpaw, tomato, wax gourd, pyracantha fortuneana fruit, lemon
Break process is carried out together, is beaten, is obtained slurries；
Chamomile, orange pericarp, mulberry leaf, Semen Coicis, oat, mung bean, grape pip are smashed into milling；
3) temperature for the slurries that step 2) obtains pulp cold treatment: is reduced to 18~20 DEG C；
4) pulp homogenizes: set-up procedure 3) the obtained pol and pH value of slurries, pol 100g/L, pH value 3.0
~3.5；
5) one time fermentation: the slurries that Hansenula yeast, kluyveromyces marxianus access step 4) obtain are fermented, dissolved oxygen amount
Control 18~20 DEG C of fermentation temperature, 5~15r/min of mixing speed, when pol is down to 10g/L, stops hair in 25~35mg/L
Ferment stands 72h；
The milling that step 2) is obtained is added to the water in 60~80 DEG C of 1~2h of heating stirring, is cooled to 20~25 DEG C, adds
Enter bifidobacterium lactis, aspergillus oryzae fermentation, fermentation temperature is 20~25 DEG C, and fermentation time is 80~100h；
6) secondary fermentation: two kinds of Preliminary fermentation liquid that step 5) is obtained mix, by lactobacillus plantarum, Lactobacillus delbrueckii,
Lactobacillus rhamnosus access is wherein fermented, and 21~25 DEG C of fermentation temperature, pol is lower than 0.5g/L, and total acid terminates hair after being greater than 6g/L
Ferment is cooled to rapidly 15 DEG C, stands 72h；
7) filter at low temperature: the fermentation liquid that step 6) is obtained diatomite filter and cardboard filter carry out low temperature mistake
Filter, filtration temperature are maintained at 10~15 DEG C；
8) low temperature is concentrated: the fermentation liquid that step 7) is obtained is concentrated, cycles of concentration using vacuum and low temperature concentrator
2 times, obtain tree peony ferment；
9) the tree peony ferment for obtaining step 8) is packed, 10 DEG C or less cryopreservations.
Preferably, in step 5), the additional amount of the Hansenula yeast and the kluyveromyces marxianus is the slurries
The 8~10% of quality.
The beneficial effects of the present invention are:
The present invention provides a kind of tree peony ferment and its production method for improving cleaning blood free radicals, with peony, ocean
Camomile, carrot, pineapple, pawpaw, tomato, orange peel, wax gourd, mulberry leaf, pyracantha fortuneana fruit, Semen Coicis, oat, mung bean, grape pip, lemon
Lemon is raw material, bifidobacterium lactis, aspergillus oryzae, Lactobacillus rhamnosus, lactobacillus plantarum, Lactobacillus delbrueckii, Hansenula yeast and mark
This kluyveromyces is strain, can be effectively reduced the loss of effective component in raw material, retains the nutrition of each raw material to greatest extent
Ingredient enriches the nutrition of peony.Ferment provided by the invention is unique in taste, and has no toxic side effect, and will not be good for human body
Health generate harm, eat after, both can get the nutrient in each raw material, long-term consumption can remove free radical, have it is anti-oxidant, by force
Power supplement is adjusted, nutrient balance in antagonism human body, excludes internal heavy metal and toxin, and oxygenation waste discharge is adjusted and is immunized, changes
Kind metabolism, enhances cell viability, decomposes aging non-viable non-apoptotic cell, manufactures neonatal cell, aging cell is made to tend to be full, promotes blood
Liquid circulation, eliminates prevention disease, and health care prolongs life.
Specific embodiment
A kind of tree peony ferment improving cleaning blood free radicals provided by the invention, including peony, chamomile, Hu Luo
Fore-telling, pineapple, pawpaw, tomato, orange peel, wax gourd, mulberry leaf, pyracantha fortuneana fruit, Semen Coicis, oat, mung bean, grape pip, lemon are raw material,
Fermented by mixed bacterium is added to form；
Mixed bacteria includes that bifidobacterium lactis, aspergillus oryzae, Lactobacillus rhamnosus, lactobacillus plantarum, Lactobacillus delbrueckii, the Chinese are inferior
Yeast and kluyveromyces marxianus.
Above-mentioned technical proposal, with peony, chamomile, carrot, pineapple, pawpaw, tomato, orange peel, wax gourd, mulberry
Leaf, pyracantha fortuneana fruit, Semen Coicis, oat, mung bean, grape pip, lemon are raw material, bifidobacterium lactis, aspergillus oryzae, Lactobacillus rhamnosus, plant
Object lactobacillus, Lactobacillus delbrueckii, Hansenula yeast and kluyveromyces marxianus are strain, can be effectively reduced in raw material effectively at
The loss divided, retains the nutritional ingredient of each raw material to greatest extent, enriches the nutrition of peony.Ferment mouthfeel provided by the invention
Uniqueness, and have no toxic side effect, harm will not be generated to the health of human body, after eating, both can get the nutrient in each raw material, for a long time
It is edible to remove free radical, have anti-oxidant, strength supplement is adjusted, nutrient balance in antagonism human body, excludes weight in vivo
Metal and toxin, oxygenation waste discharge adjust immune, improvement metabolism, and enhancing cell viability decomposes aging non-viable non-apoptotic cell, manufacture new life
Cell makes aging cell tend to be full, promotes blood circulation, and eliminates prevention disease, and health care prolongs life.
In an embodiment of the present invention, bifidobacterium lactis, aspergillus oryzae, Lactobacillus rhamnosus, lactobacillus plantarum, De Shi cream bar
The mass ratio of bacterium, Hansenula yeast and kluyveromyces marxianus is (0.5~1): (1.5~2): (0.3~0.5): (1~2):
(0.5~1): (2~3): (0.8~1.2).
In an embodiment of the present invention, peony, chamomile, pineapple, pawpaw, tomato, orange peel, wax gourd, mulberry leaf, fire
Spine fruit, Semen Coicis, oat, mung bean, grape pip and lemon mass ratio be (4~5): (0.5~0.8): (1~2): (1~2): (1
~1.5): (1~2): (0.5~1): (0.4~0.7): (0.3~0.5): (0.5~1): (1~2): (1~2): (0.8~
1.2): (0.5~0.6): (0.2~0.4).
It is including following the present invention also provides a kind of above-mentioned production method for the tree peony ferment for improving cleaning blood free radicals
Step:
1) raw material selection is cleaned: peony, carrot, tomato, wax gourd, pyracantha fortuneana fruit, lemon are after selecting by conveyer belt
Into water-bath cleaning machine, ejected wash water residual chlorine concentration is 60~80ppm, 30~60s of scavenging period；
2) pretreatment of raw material: the peony of step 1), carrot, tomato, wax gourd, pyracantha fortuneana fruit, lemon are carried out brokenly together
Broken processing, mashing, obtains slurries；
Chamomile, orange pericarp, mulberry leaf, Semen Coicis, oat, mung bean, grape pip are smashed into milling；
3) temperature for the slurries that step 2) obtains pulp cold treatment: is reduced to 18~20 DEG C；
4) pulp homogenizes: set-up procedure 3) the obtained pol and pH value of slurries, pol 100g/L, pH value 3.0
~3.5；
5) one time fermentation: the slurries that Hansenula yeast, kluyveromyces marxianus access step 4) obtain are fermented, dissolved oxygen amount
Control 18~20 DEG C of fermentation temperature, 5~15r/min of mixing speed, when pol is down to 10g/L, stops hair in 25~35mg/L
Ferment stands 72h；
The milling that step 2) is obtained is added to the water in 60~80 DEG C of 1~2h of heating stirring, is cooled to 20~25 DEG C, adds
Enter bifidobacterium lactis, aspergillus oryzae fermentation, fermentation temperature is 20~25 DEG C, and fermentation time is 80~100h；
6) secondary fermentation: the Preliminary fermentation liquid that step 5) is obtained mixes, by lactobacillus plantarum, Lactobacillus delbrueckii, sandlwood
Sugared lactobacillus access is wherein fermented, and 21~25 DEG C of fermentation temperature, pol is lower than 0.5g/L, and total acid terminates fermentation after being greater than 6g/L,
It is cooled to 15 DEG C rapidly, stands 72h；
7) filter at low temperature: the fermentation liquid that step 6) is obtained diatomite filter and cardboard filter carry out low temperature mistake
Filter, filtration temperature are maintained at 10~15 DEG C；
8) low temperature is concentrated: the fermentation liquid that step 7) is obtained is concentrated, cycles of concentration using vacuum and low temperature concentrator
2 times, obtain tree peony ferment；
9) the tree peony ferment for obtaining step 8) is packed, 10 DEG C or less cryopreservations.
Above-mentioned technical proposal, using microbial fermentation processes, the strain that screening is suitable for draft medicinal raw material ferments,
In conjunction with abstraction technique, it is different from traditional physics extraction and chemical synthesis process, not only improves nutritive value, but also can be effectively improved
Food mouthfeel.Production method of the invention is simple, easy to implement, is conducive to be mass produced.
In an embodiment of the present invention, in step 5), the additional amount of Hansenula yeast and kluyveromyces marxianus is described
The 8~10% of grind slurries quality.In order to further illustrate the present invention, clear to a kind of improvement provided by the invention with reference to embodiments
The tree peony ferment and its production method for managing blood free radicals are described in detail.
Embodiment 1
Improve cleaning blood free radicals tree peony ferment in include mass ratio be 4:0.5:1:1:1:1:0.5:0.4:0.3:
The peony of 0.5:1:1:0.8:0.5:0.2, chamomile, carrot, pineapple, pawpaw, tomato, orange peel, wax gourd, mulberry leaf,
Pyracantha fortuneana fruit, Semen Coicis, oat, mung bean, grape pip, lemon are added fermented by mixed bacterium and form；Mixed bacteria includes that mass ratio is 1:
1.5:10.3:1:0.5:2:0.8 bifidobacterium lactis, aspergillus oryzae, Lactobacillus rhamnosus, lactobacillus plantarum, Lactobacillus delbrueckii, the Chinese
Inferior yeast and kluyveromyces marxianus.
Improve the production method of the tree peony ferment of cleaning blood free radicals, comprising the following steps:
1) raw material selection is cleaned: peony, carrot, pineapple, pawpaw, tomato, wax gourd, pyracantha fortuneana fruit, lemon are by selecting
Water-bath cleaning machine is entered by conveyer belt afterwards, ejected wash water residual chlorine concentration is 60ppm, scavenging period 30s；
2) pretreatment of raw material: by the peony of step 1), carrot, pineapple, pawpaw, tomato, wax gourd, pyracantha fortuneana fruit, lemon
Break process is carried out together, is beaten, is obtained slurries；
Chamomile, orange pericarp, mulberry leaf, Semen Coicis, oat, mung bean, grape pip are smashed into milling；
3) temperature for the slurries that step 2) obtains pulp cold treatment: is reduced to 18 DEG C；
4) pulp homogenizes: set-up procedure 3) the obtained pol and pH value of slurries, pol 100g/L, pH value 3；
5) one time fermentation: the slurries that Hansenula yeast, kluyveromyces marxianus access step 4) obtain are fermented, dissolved oxygen amount
Control 18 DEG C of fermentation temperature, mixing speed 5r/min, when pol is down to 10g/L, stops fermentation, stands 72h in 25mg/L；The Chinese
The additional amount of inferior yeast and kluyveromyces marxianus is the 8% of grind slurries quality；
The milling that step 2) is obtained is added to the water in 60 DEG C of heating stirring 2h, is cooled to 20 DEG C, and newborn bifid bar is added
Bacterium, aspergillus oryzae fermentation, fermentation temperature are 25 DEG C, fermentation time 80h；6) secondary fermentation: two kinds that step 5) is obtained are initial
Fermentation liquid mixing wherein ferments lactobacillus plantarum, Lactobacillus delbrueckii, Lactobacillus rhamnosus access, and 21 DEG C of fermentation temperature, pol
Lower than 0.5g/L, total acid terminates fermentation after being greater than 6g/L, is cooled to 15 DEG C rapidly, stands 72h；
7) filter at low temperature: the fermentation liquid that step 6) is obtained diatomite filter and cardboard filter carry out low temperature mistake
Filter, filtration temperature are maintained at 10 DEG C；
8) low temperature is concentrated: the fermentation liquid that step 7) is obtained is concentrated, cycles of concentration using vacuum and low temperature concentrator
2 times, obtain tree peony ferment；
9) the tree peony ferment for obtaining step 8) is packed, 10 DEG C or less cryopreservations.
Embodiment 2
Improve cleaning blood free radicals tree peony ferment in include mass ratio be 5:0.8:2:2:1.5:2:1:0.7:0.5:
The peony of 1:2:2:1.2:0.6:0.4, chamomile, carrot, pineapple, pawpaw, tomato, orange peel, wax gourd, mulberry leaf, fire
Spine fruit, Semen Coicis, oat, mung bean, grape pip, lemon are added fermented by mixed bacterium and form；Mixed bacteria includes that mass ratio is 0.5:
The inferior ferment of bifidobacterium lactis, aspergillus oryzae, Lactobacillus rhamnosus, lactobacillus plantarum, Lactobacillus delbrueckii, the Chinese of 2:0.5:2:1:3:1.2
Female and kluyveromyces marxianus.
Improve the production method of the tree peony ferment of cleaning blood free radicals, comprising the following steps:
1) raw material selection is cleaned: peony, carrot, pineapple, pawpaw, tomato, wax gourd, pyracantha fortuneana fruit, lemon are by selecting
Water-bath cleaning machine is entered by conveyer belt afterwards, ejected wash water residual chlorine concentration is 80ppm, scavenging period 60s；
2) pretreatment of raw material: by the peony of step 1), carrot, pineapple, pawpaw, tomato, wax gourd, pyracantha fortuneana fruit, lemon
Break process is carried out together, is beaten, is obtained slurries；
Chamomile, orange pericarp, mulberry leaf, Semen Coicis, oat, mung bean, grape pip are smashed into milling；
3) temperature for the slurries that step 2) obtains pulp cold treatment: is reduced to 20 DEG C；
4) pulp homogenizes: set-up procedure 3) the obtained pol and pH value of slurries, pol 100g/L, pH value 3.0；
5) one time fermentation: the slurries that Hansenula yeast, kluyveromyces marxianus access step 4) obtain are fermented, dissolved oxygen amount
Control 20 DEG C of fermentation temperature, mixing speed 15r/min, when pol is down to 10g/L, stops fermentation, stands 72h in 35mg/L；The Chinese
The additional amount of inferior yeast and kluyveromyces marxianus is the 10% of grind slurries quality；
6) secondary fermentation: two kinds of Preliminary fermentation liquid that step 5) is obtained mix, by lactobacillus plantarum, Lactobacillus delbrueckii,
Lactobacillus rhamnosus access is wherein fermented, and 25 DEG C of fermentation temperature, pol is lower than 0.5g/L, and total acid terminates fermentation after being greater than 6g/L,
It is cooled to 15 DEG C rapidly, stands 72h；
The milling that step 2) is obtained is added to the water in 80 DEG C of heating stirring 1h, is cooled to 20 DEG C, and newborn bifid bar is added
Bacterium, aspergillus oryzae fermentation, fermentation temperature are 225 DEG C, fermentation time 100h；
7) filter at low temperature: the fermentation liquid that step 6) is obtained diatomite filter and cardboard filter carry out low temperature mistake
Filter, filtration temperature are maintained at 15 DEG C；
8) low temperature is concentrated: the fermentation liquid that step 7) is obtained is concentrated, cycles of concentration using vacuum and low temperature concentrator
2 times, obtain tree peony ferment；
9) the tree peony ferment for obtaining step 8) is packed, 10 DEG C or less cryopreservations.
Embodiment 3
Improve cleaning blood free radicals tree peony ferment in include mass ratio be 4.5:0.65:1.5:1.5:1.2:1.5:
Peony, chamomile, carrot, pineapple, pawpaw, tomato, the mandarin orange of 0.8:0.55:0.4:0.75:1.5:1.5:1:0.55:0.3
Tangerine pericarp, wax gourd, mulberry leaf, pyracantha fortuneana fruit, Semen Coicis, oat, mung bean, grape pip, lemon are added fermented by mixed bacterium and form；Mixing
Strain includes bifidobacterium lactis, the aspergillus oryzae, Lactobacillus rhamnosus, plant that mass ratio is 0.8:1.8:0.4:1.5:0.75:2.5:1
Object lactobacillus, Lactobacillus delbrueckii, Hansenula yeast and kluyveromyces marxianus.
Improve the production method of the tree peony ferment of cleaning blood free radicals, comprising the following steps:
1) raw material selection is cleaned: peony, carrot, pineapple, pawpaw, tomato, wax gourd, pyracantha fortuneana fruit, lemon are by selecting
Water-bath cleaning machine is entered by conveyer belt afterwards, ejected wash water residual chlorine concentration is 70ppm, scavenging period 40s；
2) pretreatment of raw material: the peony of step 1), carrot, tomato, wax gourd, pyracantha fortuneana fruit, lemon are carried out brokenly together
Broken processing, mashing, obtains slurries；
Chamomile, orange pericarp, mulberry leaf, Semen Coicis, oat, mung bean, grape pip are smashed into milling；
3) temperature for the slurries that step 2) obtains pulp cold treatment: is reduced to 19 DEG C；
4) pulp homogenizes: set-up procedure 3) the obtained pol and pH value of slurries, pol 100g/L, pH value 3.5；
5) one time fermentation: the slurries that Hansenula yeast, kluyveromyces marxianus access step 4) obtain are fermented, dissolved oxygen amount
Control 19 DEG C of fermentation temperature, mixing speed 10r/min, when pol is down to 10g/L, stops fermentation, stands 72h in 30mg/L；The Chinese
The additional amount of inferior yeast and kluyveromyces marxianus is the 9% of grind slurries quality；
The milling that step 2) is obtained is added to the water in 70 DEG C of heating stirring 1.5h, is cooled to 25 DEG C, and newborn bifid is added
Bacillus, aspergillus oryzae fermentation, fermentation temperature are 20 DEG C, fermentation time 90h；
6) secondary fermentation: two kinds of Preliminary fermentation liquid that step 5) is obtained mix, by lactobacillus plantarum, Lactobacillus delbrueckii,
Lactobacillus rhamnosus access is wherein fermented, and 23 DEG C of fermentation temperature, pol is lower than 0.5g/L, and total acid terminates fermentation after being greater than 6g/L,
It is cooled to 15 DEG C rapidly, stands 72h；
7) filter at low temperature: the fermentation liquid that step 6) is obtained diatomite filter and cardboard filter carry out low temperature mistake
Filter, filtration temperature are maintained at 12 DEG C；
8) low temperature is concentrated: the fermentation liquid that step 7) is obtained is concentrated, cycles of concentration using vacuum and low temperature concentrator
2 times, obtain tree peony ferment；
9) the tree peony ferment for obtaining step 8) is packed, 10 DEG C or less cryopreservations.
Measure the Examples 1 to 3 tree peony ferment obtained for improving cleaning blood free radicals and benefit boat enzyme stoste (control
Example) in SOD activity, the results are shown in Table 1.
The active experimental result of 1 SOD of table
Measuring improves the DPPH inhibiting rate for clearing up the tree peony ferment of blood free radicals made from Examples 1 to 3 seeks with main
Substance, nutriment such as total phenol, general flavone, total starches, crude protein content are supported, antioxygenic property is characterized using DPPH inhibiting rate,
It the results are shown in Table 2.Wherein, determining total phenol uses welfare colorimetric method, and Determination of Total Flavonoids uses liquid chromatography, and total starches measurement uses
Anthrone colorimetry, crude protein content measurement use micro triumphant formula nitriding, and oxidation-reduction quality uses DPPH method, to free radical
Clearance rate be index, detected antioxygenic property.
, the results are shown in Table 2.
The experimental result of table 2 nutriment and DPPH inhibiting rate
The tree peony ferment of improvement cleaning blood free radicals made from Examples 1 to 3 is subjected to eating effect verifying, random skin
Skin has the patient of color spot, and the condition-inference of patient accepts and believe the Illnesses Diagnoses that regular hospital provides, and diagnostic result is going out for color spot
It is now and caused by free-radical contents are high in blood.200 people are selected, are divided into 4 groups, including experimental group 1~3 and control group, often
50 people of group；Control group normal diet, experimental group 1~3 also eat changing for Examples 1 to 3 in addition to the diet according to control group respectively
The tree peony ferment of kind cleaning blood free radicals.Experimental group 1~3 everyone each taking 30mL, daily breakfast, late one after each meal, often
It is secondary must be diluted with 5~8 times of warm waters after take, be repeated in the morning, afternoon and evening with water daily, continuously take two weeks, surrounding paid a return visit and remembered
Effect is recorded, the effect of color spot symptom, which is divided into, to be restored normal, is clearly better, improves and without positive effect, count different-effect respectively
Number, the results are shown in Table 3 and table 4.
Wherein curative effect judging standard is as follows:
Restore normal: color spot ill symptoms disappear, and body surface restores normal；
Be clearly better: color spot symptom disappears；
Improve: color spot symptom gradually mitigates；
Without positive effect: color spot symptom is without improvement.
Table 3 is continuously taken two weeks effects and is compared
| Restore normal/people | Be clearly better/people | Improvement/people | Without positive effect/people | |
| Experimental group 1 | 18 | 24 | 5 | 3 |
| Experimental group 2 | 16 | 26 | 6 | 2 |
| Experimental group 3 | 17 | 27 | 4 | 2 |
| Control group | 0 | 0 | 6 | 44 |
Table 4 is continuously taken surrounding effect and is compared
| Restore normal/people | Be clearly better/people | Improvement/people | Without positive effect/people | |
| Experimental group 1 | 34 | 9 | 7 | 0 |
| Experimental group 2 | 31 | 10 | 9 | 0 |
| Experimental group 3 | 32 | 8 | 10 | 0 |
| Control group | 0 | 2 | 14 | 34 |
Disclosed above is only specific implementation of the patent example, but this patent is not limited to this, for the general of this field
For logical technical staff, without departing from the principle of the present invention, the deformation made should be regarded as belonging to the scope of the present invention.
Claims (5)
1. a kind of tree peony ferment for improving cleaning blood free radicals, which is characterized in that including peony, chamomile, carrot, spinach
Trailing plants, pawpaw, tomato, orange peel, wax gourd, mulberry leaf, pyracantha fortuneana fruit, Semen Coicis, oat, mung bean, grape pip, lemon are raw material, are added
Fermented by mixed bacterium forms；
The mixed bacteria includes that bifidobacterium lactis, aspergillus oryzae, Lactobacillus rhamnosus, lactobacillus plantarum, Lactobacillus delbrueckii, the Chinese are inferior
Yeast and kluyveromyces marxianus.
2. tree peony ferment as described in claim 1, which is characterized in that the bifidobacterium lactis, the aspergillus oryzae, the sandlwood
The matter of sugared lactobacillus, the lactobacillus plantarum, the Lactobacillus delbrueckii, the Hansenula yeast and the kluyveromyces marxianus
Amount is than being (0.5~1): (1.5~2): (0.3~0.5): (1~2): (0.5~1): (2~3): (0.8~1.2).
3. tree peony ferment as described in claim 1, which is characterized in that the peony, the chamomile, the carrot,
The pineapple, the pawpaw, the tomato, the orange peel, the wax gourd, the mulberry leaf, the pyracantha fortuneana fruit, the heart of a lotus seed
Rice, the oat, the mung bean, the grape pip and the mass ratio of the lemon are (4~5): (0.5~0.8): (1~2):
(1~2): (1~1.5): (1~2): (0.5~1): (0.4~0.7): (0.3~0.5): (0.5~1): (1~2): (1~2):
(0.8~1.2): (0.5~0.6): (0.2~0.4).
4. a kind of production method for the tree peony ferment for improving cleaning blood free radicals as described in any one of claims 1 to 3, special
Sign is, comprising the following steps:
1) raw material selection clean: peony, carrot, pineapple, pawpaw, tomato, wax gourd, pyracantha fortuneana fruit, lemon after selecting by
Conveyer belt enters water-bath cleaning machine, and ejected wash water residual chlorine concentration is 60~80ppm, 30~60s of scavenging period；
2) pretreatment of raw material: together by the peony of step 1), carrot, pineapple, pawpaw, tomato, wax gourd, pyracantha fortuneana fruit, lemon
Break process is carried out, mashing obtains slurries；
Chamomile, orange pericarp, mulberry leaf, Semen Coicis, oat, mung bean, grape pip are smashed into milling；
3) temperature for the slurries that step 2) obtains pulp cold treatment: is reduced to 18~20 DEG C；
4) pulp homogenizes: set-up procedure 3) the obtained pol and pH value of slurries, pol 100g/L, pH value is 3.0~
3.5；
5) one time fermentation: the slurries that Hansenula yeast, kluyveromyces marxianus access step 4) obtain are fermented, dissolved oxygen amount control
In 25~35mg/L, 18~20 DEG C of fermentation temperature, 5~15r/min of mixing speed, when pol is down to 10g/L, stop fermentation, it is quiet
Set 72h；
The milling that step 2) is obtained is added to the water in 60~80 DEG C of 1~2h of heating stirring, is cooled to 20~25 DEG C, and cream is added
Bifidobacterium, aspergillus oryzae fermentation, fermentation temperature are 20~25 DEG C, and fermentation time is 80~100h；
6) secondary fermentation: two kinds of Preliminary fermentation liquid that step 5) is obtained mix, by lactobacillus plantarum, Lactobacillus delbrueckii, sandlwood
Sugared lactobacillus access is wherein fermented, and 21~25 DEG C of fermentation temperature, pol is lower than 0.5g/L, and total acid terminates fermentation after being greater than 6g/L,
It is cooled to 15 DEG C rapidly, stands 72h；
7) filter at low temperature: the fermentation liquid that step 6) is obtained diatomite filter and cardboard filter carry out filter at low temperature, mistake
Filter temperature is maintained at 10~15 DEG C；
8) low temperature is concentrated: the fermentation liquid that step 7) is obtained is concentrated using vacuum and low temperature concentrator, and 2 times of cycles of concentration,
Obtain tree peony ferment；
9) the tree peony ferment for obtaining step 8) is packed, 10 DEG C or less cryopreservations.
5. production method as claimed in claim 4, which is characterized in that in step 5), the Hansenula yeast and the Marx
The additional amount of kluyveromyces is the 8~10% of the grind slurries quality.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754658A (en) * | 2019-10-25 | 2020-02-07 | 宇凌 | Peony fresh flower enzyme and preparation method thereof |
| CN114107082A (en) * | 2020-08-27 | 2022-03-01 | 安琪酵母股份有限公司 | Complex microbial inoculant and application thereof |
| CN115624583A (en) * | 2022-12-19 | 2023-01-20 | 贵州佳义生物科技开发有限公司 | Red seed leavening and application thereof |
-
2019
- 2019-02-27 CN CN201910147630.4A patent/CN109700018A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110754658A (en) * | 2019-10-25 | 2020-02-07 | 宇凌 | Peony fresh flower enzyme and preparation method thereof |
| CN114107082A (en) * | 2020-08-27 | 2022-03-01 | 安琪酵母股份有限公司 | Complex microbial inoculant and application thereof |
| CN114107082B (en) * | 2020-08-27 | 2024-03-15 | 安琪酵母股份有限公司 | Composite microbial agent and application thereof |
| CN115624583A (en) * | 2022-12-19 | 2023-01-20 | 贵州佳义生物科技开发有限公司 | Red seed leavening and application thereof |
| CN115624583B (en) * | 2022-12-19 | 2024-04-30 | 贵州佳义生物科技开发有限公司 | Red seed ferment and application thereof |
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