CN105559084B - Tree peony ferment and preparation method and application - Google Patents

Tree peony ferment and preparation method and application Download PDF

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Publication number
CN105559084B
CN105559084B CN201510976037.2A CN201510976037A CN105559084B CN 105559084 B CN105559084 B CN 105559084B CN 201510976037 A CN201510976037 A CN 201510976037A CN 105559084 B CN105559084 B CN 105559084B
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tree peony
ferment
fermentation
peony
liquid
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CN201510976037.2A
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CN105559084A (en
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不公告发明人
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李�杰
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; THEIR TREATMENT, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The present invention relates to one kind with one or both of peony or compounding moutan bark, peony meal for raw material, the method for preparing tree peony ferment by material processing, low temperature sterilization, grinding mashing, fermentation, after-ripening, filtering, ultra-high static pressure sterilizing etc., and the application of tree peony ferment and the tree peony ferment that this method is prepared.The tree peony ferment that the method for the present invention is prepared utilizes low temperature sterilization, strictly controls fermentation temperature, using being implanted into zymophyte three times, effectively reduce the loss of active ingredient in tree peony ferment, it is suppressed that the growth of other miscellaneous bacterias remains tree peony enzyme activity to greatest extent.Meanwhile tree peony ferment have reduce cholesterol, blood pressure lowering, toxin-expelling and face nourishing, it is antifatigue, enhance human immunity, adjust human physiological functions and other effects advantage, in food industry, health products and medicine and other fields with wide development prospect.

Description

Tree peony ferment and preparation method and application

Technical field

The invention belongs to food processing technology fields, and in particular to a kind of tree peony ferment and preparation method thereof, the tree peony ferment The application of element.

Background technology

Tree peony(Paeonia suffruticosa Andr.)Belong to Ranunculaceae Paeonia, machaka has very high sight Reward value and pharmaceutical value.The Chinese Academy of Sciences using chemical assay the result shows that, contain 8 in peony petal, pistil and pollen Kind amino acid and multivitamin needed by human, carbohydrate and Flavonoid substances, also containing there are many enzyme, 7 kinds of macroelements and 5 Trace element necessary to kind human body has anti-cancer, anticancer, adjusts blood fat, stabilizing blood pressure, toxin-expelling and face nourishing, anti-aging, raising The effects that body immunity.The root bark of tree peony is the dry root skin of tree peony, first recorded in《Sheng Nong's herbal classic》, have the medicinal of more than 2000 years History, is one of the conventional Chinese medicine kind of Chinese Pharmacopoeia collection, cold nature, hides bitter, pungent, with clearing heat and cooling blood, is activated blood circulation and dispersed blood clots Effect, modern pharmacological research show that moutan bark, which has, to be adjusted cardiovascular system, anticonvulsion, liver protection, adjusts urinary system, adjust Immunocyte, antimicrobial antiphlogistic effect etc..

Peony seed oil was approved as new resource food in 2011 by the Ministry of Public Health, and hereafter peony seed oil enters mass production rank Section.By will produce a large amount of peony seeds grouts after using squeezing method or lixiviation process to prepare peony seed oil after decortication, account for about tree peony The one third of seed total amount, containing 5-15% fat in these seedcake dregs of rice, 30-40% albumen, also contain Chinese herbaceous peony glycoside, benzoic acid, Polysaccharide isoreactivity ingredient.Phoenix peony was approved as new raw-food material in 2013 by the Ministry of Public Health, and peony, which is important, to open Hair utilizes resource, and every year in addition to ornamental, a large amount of fresh peony flowers are descended slowly and lightly, wasted, do not developed and utilized to it naturally, Cause the waste of precious resources.For a long time, the utilization of tree peony deep processed product but seem that serious lag, production are additional Value is very low, does not also form industrial chain.Therefore make full use of activity and nutritional ingredient in these residues oily to comprehensively utilizing With tree peony, improves tree peony industry added value and be of great significance.

With deepening continuously for microbe research, ferment has increasingly been recognized the beneficial effect of health by people Know.Ferment is a kind of large biological molecule substance with special bioactivity being made of amino acid, it is present in all work It is to maintain body normal function in animal and plant body, digestion food, a kind of necessary material of the vital movements such as repair tissue, almost Participate in all vital movements:It ponders a problem, moves, sleep, breathing, indignation, sobbing or secretion hormone etc. are all with ferment Action result centered on element.The catalytic action of ferment catalyst urges the biochemical reaction of body, urges biological phenomena It carries out.If without ferment, biochemical reaction will be unable to carry out, five big nutrients(Carbohydrate, lipoids, protein, dimension life Element, minerals)All that change is utterly useless to body, biological phenomena will stop.Therefore, ferment does not say the importance of life And explain, or even it is known as " substance to live ", " substance for grasping all vital movements " by many people.There is ferment oxidation to make With, decomposition, metabolism, thermal energy effect, the effect of purification blood and antibacterial defence effect.But it is retrieved in applicant In the range of, it is the tree peony ferment product of raw material there is not yet document report using tree peony.

Invention content

The technical problem to be solved by the present invention is in view of the deficiencies of the prior art, provide a kind of tree peony ferment, and prepare Method and related application.

The present invention provides a kind of preparation method of tree peony ferment, and the method is with peony or compounding moutan bark, tree peony One or both of grouts are prepared as a raw material.Further, the peony, moutan bark or peony meal derive from Feng Dan Tree peony or Paeonia papaveracea.

Further, the tree peony ferment is former with one or both of peony or compounding moutan bark, peony meal Material sterilizes by material processing, low temperature sterilization, grinding mashing, fermentation, after-ripening, filtering, ultra-high static pressure, obtains liquid tree peony ferment Element.Or obtain solid tree peony ferment using cryogenic vacuum concentration, freeze-drying.

Further, the material processing, is separately to handle peony, moutan bark or peony meal.

Preferably, the peony is to pick the tree peony that half-open in full bloom from field, flower is intact, fresh and tender, disease-free Flower, plucking time is, after 4 o'clock of afternoon at o'clock in the morning 6-10, and removes foreign matter, is cleaned in sterile water, is drained for use.

Preferably, the moutan bark or peony meal are to dry moutan bark or peony meal to moisture≤7wt%, Drying condition is 50 DEG C hereinafter, be then crushed to 200 mesh or more, obtains Cortex Moutan micropowder or peony meal micro mist is for use.

Further, the low temperature sterilization is peony, Cortex Moutan micropowder or peony meal micro mist, low at 50-75 DEG C Temperature sterilizing 20-40min.

Further, the grinding mashing is peony or compounding Cortex Moutan micropowder, peony meal micro mist in proportion One or both of, the sterile water of 2-5 times of addition after being uniformly mixed, is mixed object and is placed in grinder, be ground into Tree peony zymotic fluid, zymotic fluid granularity are set as 80-100 mesh, are stored in 25-30 DEG C of environment, for use.

Preferably, the peony:The weight ratio of Cortex Moutan micropowder and/or peony meal micro mist is 10:1-4.

Preferably, the peony:Cortex Moutan micropowder:The weight ratio of peony meal micro mist is 20:1-3:1:4.

Further, the fermentation is to include three saccharomycetes to make fermentation, lactobacillus-fermented, fermenting bacillus natto processes.

Preferably, the saccharomycetes to make fermentation is to access the saccharomycete seed liquor of tree peony zymotic fluid 10-15%(Per ml kinds 5*10 containing saccharomycete in sub- liquid7CFU), stir evenly, in 25-35 DEG C of fermentation 10-15d of temperature, when the PH of fermentation system is reached Fermentation is terminated when 4.0, obtains saccharomycetes to make fermentation liquid.

It is furthermore preferred that the saccharomycete includes in Hansenula anomala, saccharomyces cerevisiae, Lu Shi Zygosaccharomyces and Weir yeast It is one or more.

Preferably, the lactobacillus-fermented is that the lactobacillus solution of 5-10% for saccharomycetes to make fermentation liquid is added(Often 5*10 containing lactic acid bacteria in ml seed liquors8CFU), stir evenly, in 35-45 DEG C of fermentation 15-30d of temperature, when pH reaches 4.3 Fermentation is terminated, streptococcus acidi lactici fermented solution is obtained.

It is furthermore preferred that the lactic acid bacteria include lactobacillus plantarum, lactobacillus fermenti, Leuconostoc mesenteroides, streptococcus thermophilus, It is one or more in Bifidobacterium, lactobacillus bulgaricus and lactobacillus acidophilus.

Preferably, the fermenting bacillus natto is that the Bacillus natto seed liquor of 8-15% for streptococcus acidi lactici fermented solution is added(Often 4.5*10 containing Bacillus natto in ml seed liquors7CFU), stir evenly, in 30-40 DEG C of fermentation 30-60d of temperature, obtain fermenting bacillus natto Liquid.

It is furthermore preferred that the Bacillus natto is bafillus natto.

Further, the after-ripening is fermenting bacillus natto liquid being placed in refrigerated storage tank preservation 60-110d at 8-15 DEG C.

Further, the filtering is that by the feed liquid after after-ripening, supernatant is extracted in first siphon, then is filtered through 0.22 μm of micropore Film plate and frame filter press is filtered.

Further, the ultra-high static pressure sterilizing is that device therefor ultra-high static pressure pressure is 200-400Mpa, processing time It is 20-40min to get liquid tree peony ferment.

Further, the liquid tree peony ferment, concentrates by cryogenic vacuum, is freeze-dried at a temperature of -40 DEG C and obtains solid Tree peony ferment.

The present invention also provides a kind of tree peony ferment, are prepared by any of the above-described method.

Further, the tree peony ferment is liquid tree peony ferment or solid tree peony ferment.

Preferably, the tree peony ferment be powder or be further processed on powder base particle, oral solution, tablet, The forms such as capsule.

The present invention also provides a kind of products including above-mentioned tree peony ferment.

The present invention also provides a kind of purposes of tree peony ferment in preparing product.

Preferably, the product is food, health products and beautification product etc..

The tree peony ferment that the method for the present invention is prepared utilizes low temperature sterilization or ultra-high static pressure sterilizing, stringent control fermentation temperature Degree, effectively reduces the loss of active ingredient in tree peony ferment, remains tree peony enzyme activity to greatest extent, is free of anyone Work essence, pigment, product are safe to use effectively.Using zymophyte is implanted into three times, the growth of other miscellaneous bacterias is effectively inhibited, is ensured The stabilization of product quality, while being conducive to be promoted the synthetic activity of ferment product.The active principle of product of the present invention is not viable bacteria, But the low molecule active constituent of stabilization that viable bacteria generates, such as low molecular peptide, oligosaccharides, saponin(e, the isoflavones isoreactivity factor, therefore not Need Cord blood, long shelf-life.Meanwhile tree peony ferment has reduction cholesterol, blood pressure lowering, toxin-expelling and face nourishing, antifatigue, enhancing people Body immunity adjusts human physiological functions and other effects advantage, has wide open in food industry, health products and medicine and other fields Send out application prospect.

Specific implementation mode

It being further illustrated the present invention with reference to embodiment, it should be understood that these examples cannot function as the limitation of the present invention, Without departing from the spirit and substance of the case in the present invention, modifications or substitutions made by all belong to the scope of the present invention.If not especially It indicates, the means in following embodiments are conventional means known in the art.

It is prepared by 1 tree peony zymotic fluid of embodiment.

Step 1: peony is picked.

After, 4 o'clock of afternoon, picking field is half-open in full bloom, flower is intact, fresh and tender, disease-free at o'clock in the morning 6-10 Peony, and remove foreign matter, cleaned in sterile water, drained for use.

Step 2: low temperature sterilization.

By peony in 75 DEG C of low temperature sterilization 20min.

Step 3: grinding mashing.

The peony after low temperature sterilization is chosen, the sterile water of 2 times of addition after being uniformly mixed, is mixed object and is placed in In grinder, it is ground into tree peony zymotic fluid, zymotic fluid granularity is set as 80 mesh, is stored in 25-30 DEG C of environment, for use.

It is prepared by 2 tree peony zymotic fluid of embodiment.

Step 1: peony is picked.

After, 4 o'clock of afternoon, picking field is half-open in full bloom, flower is intact, fresh and tender, disease-free at o'clock in the morning 6-10 Peony, and remove foreign matter, cleaned in sterile water, drained for use.

Step 2: low temperature sterilization.

By peony in 50 DEG C of low temperature sterilization 40min.

Step 3: grinding mashing.

The peony after low temperature sterilization is chosen, the sterile water of 5 times of addition after being uniformly mixed, is mixed object and is placed in In grinder, it is ground into tree peony zymotic fluid, zymotic fluid granularity is set as 100 mesh, is stored in 25-30 DEG C of environment, for use.

It is prepared by 3 tree peony zymotic fluid of embodiment.

Step 1: peony is picked.

After, 4 o'clock of afternoon, picking field is half-open in full bloom, flower is intact, fresh and tender, disease-free at o'clock in the morning 6-10 Peony, and remove foreign matter, cleaned in sterile water, drained for use.

Step 2: moutan bark is handled.

Moutan bark to be dried to moisture≤7wt%, drying condition is 50 DEG C hereinafter, being then crushed to 200 mesh or more, It is for use to obtain Cortex Moutan micropowder.

Step 3: low temperature sterilization.

By peony, Cortex Moutan micropowder, in 75 DEG C of low temperature sterilization 20min.

Step 4: grinding mashing.

By weight 10:1 chooses peony, Cortex Moutan micropowder, adds the sterile water of 2 times of above-mentioned material, is stirred It after even, be mixed object and be placed in grinder, be ground into tree peony zymotic fluid, zymotic fluid granularity is set as 80 mesh, in 25-30 DEG C It is stored in environment, for use.

It is prepared by 4 tree peony zymotic fluid of embodiment.

Step 1: peony is picked.

After, 4 o'clock of afternoon, picking field is half-open in full bloom, flower is intact, fresh and tender, disease-free at o'clock in the morning 6-10 Peony, and remove foreign matter, cleaned in sterile water, drained for use.

Step 2: moutan bark is handled.

Moutan bark to be dried to moisture≤7wt%, drying condition is 50 DEG C hereinafter, being then crushed to 200 mesh or more, It is for use to obtain Cortex Moutan micropowder.

Step 3: low temperature sterilization.

By peony, Cortex Moutan micropowder, in 50 DEG C of low temperature sterilization 40min.

Step 4: grinding mashing.

By weight 10:4 choose peony, Cortex Moutan micropowder, add the sterile water of 5 times of above-mentioned material, are stirred It after even, be mixed object and be placed in grinder, be ground into tree peony zymotic fluid, zymotic fluid granularity is set as 100 mesh, in 25-30 DEG C Environment in store, for use.

It is prepared by 5 tree peony zymotic fluid of embodiment.

Step 1: peony is picked.

After, 4 o'clock of afternoon, picking field is half-open in full bloom, flower is intact, fresh and tender, disease-free at o'clock in the morning 6-10 Peony, and remove foreign matter, cleaned in sterile water, drained for use.

Step 2: peony meal is handled.

Peony meal is dried to moisture≤7wt%, drying condition be 50 DEG C hereinafter, be then crushed to 200 mesh with On, it is for use to obtain peony meal micro mist.

Step 3: low temperature sterilization.

By peony, peony meal micro mist, in 75 DEG C of low temperature sterilization 20min.

Step 4: grinding mashing.

By weight 10:1 chooses peony, peony meal micro mist, adds the sterile water of 2 times of above-mentioned material, is stirred After uniformly, it is mixed object and is placed in grinder, be ground into tree peony zymotic fluid, zymotic fluid granularity is set as 100 mesh, in 25-30 DEG C environment in store, for use.

It is prepared by 6 tree peony zymotic fluid of embodiment.

Step 1: peony is picked.

After, 4 o'clock of afternoon, picking field is half-open in full bloom, flower is intact, fresh and tender, disease-free at o'clock in the morning 6-10 Peony, and remove foreign matter, cleaned in sterile water, drained for use.

Step 2: peony meal micro mist is handled.

Peony meal is dried to moisture≤7wt%, drying condition be 50 DEG C hereinafter, be then crushed to 200 mesh with On, it is for use to obtain peony meal micro mist.

Step 3: low temperature sterilization.

By peony, peony meal micro mist, in 50 DEG C of low temperature sterilization 40min.

Step 4: grinding mashing.

By weight 10:4 choose peony, peony meal micro mist, add the sterile water of 5 times of above-mentioned material, are stirred After uniformly, it is mixed object and is placed in grinder, be ground into tree peony zymotic fluid, zymotic fluid granularity is set as 80 mesh, in 25-30 DEG C Environment in store, for use.

It is prepared by 7 tree peony zymotic fluid of embodiment.

Step 1: peony is picked.

After, 4 o'clock of afternoon, picking field is half-open in full bloom, flower is intact, fresh and tender, disease-free at o'clock in the morning 6-10 Peony, and remove foreign matter, cleaned in sterile water, drained for use.

Step 2: moutan bark and peony meal processing.

Moutan bark, peony meal are dried to moisture≤7wt%, drying condition is 50 DEG C hereinafter, being then crushed to It more than 200 mesh, obtains Cortex Moutan micropowder and peony meal micro mist is for use.

Step 3: low temperature sterilization.

By peony, Cortex Moutan micropowder, peony meal micro mist, in 75 DEG C of low temperature sterilization 20min.

Step 4: grinding mashing.

By weight 20:1:4 choose peony, Cortex Moutan micropowder, peony meal micro mist, add the nothing of 2 times of above-mentioned material Bacterium water after being uniformly mixed, is mixed object and is placed in grinder, is ground into tree peony zymotic fluid, and zymotic fluid granularity is set as 80 mesh are stored, for use in 25-30 DEG C of environment.

It is prepared by 8 tree peony zymotic fluid of embodiment.

Step 1: peony is picked.

After, 4 o'clock of afternoon, picking field is half-open in full bloom, flower is intact, fresh and tender, disease-free at o'clock in the morning 6-10 Peony, and remove foreign matter, cleaned in sterile water, drained for use.

Step 2: moutan bark and peony meal processing.

Moutan bark, peony meal are dried to moisture≤7wt%, drying condition is 50 DEG C hereinafter, being then crushed to It more than 200 mesh, obtains Cortex Moutan micropowder and peony meal micro mist is for use.

Step 3: low temperature sterilization.

By peony, Cortex Moutan micropowder, peony meal micro mist, in 50 DEG C of low temperature sterilization 40min.

Step 4: grinding mashing.

By weight 20:3:1 chooses peony, Cortex Moutan micropowder, peony meal micro mist, adds the nothing of 5 times of above-mentioned material Bacterium water after being uniformly mixed, is mixed object and is placed in grinder, is ground into tree peony zymotic fluid, and zymotic fluid granularity is set as 100 mesh are stored, for use in 25-30 DEG C of environment.

It is prepared by 9 liquid tree peony ferment of embodiment.

Step 1: saccharomycetes to make fermentation.

By any tree peony zymotic fluids of embodiment 1-8,10% S. cervisiae seed liquor is added(Contain in per ml seed liquors S. cervisiae 5*107CFU), stir evenly, at 25-35 DEG C of fermentation 15d of temperature, the end when the PH of fermentation system reaches 4.0 It only ferments, obtains saccharomycetes to make fermentation liquid.

Step 2: lactobacillus-fermented.

By saccharomycetes to make fermentation liquid, 10% lactobacillus plantarum seed liquor is added(Contain lactobacillus plantarum in per ml seed liquors 5*108CFU), stir evenly, in 35-45 DEG C of fermentation 15d of temperature, fermentation terminated when pH reaches 4.3, obtains lactobacillus-fermented Liquid.

Step 3: fermenting bacillus natto.

By streptococcus acidi lactici fermented solution, 8% bafillus natto seed liquor is added(Contain natto gemma bar in per ml seed liquors Bacterium 4.5*107CFU), stir evenly, in 30-40 DEG C of fermentation 60d of temperature, obtain fermenting bacillus natto liquid.

Step 4: after-ripening.

Fermenting bacillus natto liquid is placed in refrigerated storage tank preservation 60d at 8-15 DEG C.

Step 5: filtering.

By the feed liquid after after-ripening, supernatant is extracted in first siphon, then is filtered through 0.22 μm of miillpore filter plate and frame filter press.

Step 6: ultra-high static pressure sterilizes.

Ultra-high static pressure sterilizes, and device therefor ultra-high static pressure pressure is 400Mpa, and processing time is that 20min is male to get liquid Red ferment.

It is prepared by 10 liquid tree peony ferment of embodiment.

Step 1: saccharomycetes to make fermentation.

By any tree peony zymotic fluids of embodiment 1-8,15% Hansenula anomala seed liquor is added(Per in ml seed liquors Contain Hansenula anomala 5*107CFU), stir evenly, in 25-35 DEG C of fermentation 10d of temperature, when the PH of fermentation system is reached Fermentation is terminated when 4.0, obtains saccharomycetes to make fermentation liquid.

Step 2: lactobacillus-fermented.

By saccharomycetes to make fermentation liquid, 5% lactobacillus fermenti seed liquor is added(Contain lactobacillus fermenti 5* in per ml seed liquors 108CFU), stir evenly, in 35-45 DEG C of 30 d of fermentation of temperature, fermentation terminated when pH reaches 4.3, obtains lactobacillus-fermented Liquid.

Step 3: fermenting bacillus natto.

By streptococcus acidi lactici fermented solution, 15% bafillus natto seed liquor is added(Contain natto gemma in per ml seed liquors Bacillus 4.5*107CFU), stir evenly, in 30-40 DEG C of fermentation 30d of temperature, obtain fermenting bacillus natto liquid.

Step 4: after-ripening.

Fermenting bacillus natto liquid is placed in refrigerated storage tank preservation 110d at 8-15 DEG C.

Step 5: filtering.

By the feed liquid after after-ripening, supernatant is extracted in first siphon, then is filtered through 0.22 μm of miillpore filter plate and frame filter press.

Step 6: ultra-high static pressure sterilizes.

Ultra-high static pressure sterilizes, and device therefor ultra-high static pressure pressure is 200Mpa, and processing time is that 40min is male to get liquid Red ferment.

It is prepared by 11 liquid tree peony ferment of embodiment.

Step 1: saccharomycetes to make fermentation.

By any tree peony zymotic fluids of embodiment 1-8,12% mixed yeast seed liquor is added, wherein mixed yeast Lu Shi Zygosaccharomyces bacterium and Weir saccharomycete volume ratio are 1 in seed liquor:1(Contain per mL in Lu Shi Zygosaccharomyces bacterium seed liquors Lu Shi Zygosaccharomyces bacterium 5 × 107Cfu contains Weir saccharomycete 5 × 10 in Weir saccharomycete seed liquor per mL7cfu), stirring Uniformly, in 25-35 DEG C of fermentation 15d of temperature, fermentation is terminated when the PH of fermentation system reaches 4.0, obtains saccharomycetes to make fermentation liquid.

Step 2: lactobacillus-fermented.

By saccharomycetes to make fermentation liquid, 8% Leuconostoc mesenteroides seed liquor is added(Contain the bright beading of goldbeater's skin in per ml seed liquors Bacterium 5*108CFU), stir evenly, in 35-45 DEG C of fermentation 15d of temperature, fermentation terminated when pH reaches 4.3, obtains lactic acid bacteria hair Zymotic fluid.

Step 3: fermenting bacillus natto.

By streptococcus acidi lactici fermented solution, 8% bafillus natto seed liquor is added(Contain natto gemma bar in per ml seed liquors Bacterium 4.5*107CFU), stir evenly, in 30-40 DEG C of fermentation 60d of temperature, obtain fermenting bacillus natto liquid.

Step 4: after-ripening.

Fermenting bacillus natto liquid is placed in refrigerated storage tank preservation 60d at 8-15 DEG C.

Step 5: filtering.

By the feed liquid after after-ripening, supernatant is extracted in first siphon, then is filtered through 0.22 μm of miillpore filter plate and frame filter press.

Step 6: ultra-high static pressure sterilizes.

Ultra-high static pressure sterilizes, and device therefor ultra-high static pressure pressure is 400Mpa, and processing time is that 20min is male to get liquid Red ferment.

It is prepared by 12 liquid tree peony ferment of embodiment.

Step 1: saccharomycetes to make fermentation.

By any tree peony zymotic fluids of embodiment 1-8,10% S. cervisiae seed liquor is added(Contain in per ml seed liquors S. cervisiae 5*107CFU), stir evenly, at 25-35 DEG C of fermentation 10d of temperature, the end when the PH of fermentation system reaches 4.0 It only ferments, obtains saccharomycetes to make fermentation liquid.

Step 2: lactobacillus-fermented.

By saccharomycetes to make fermentation liquid, 10% streptococcus thermophilus seed liquor is added(Contain streptococcus thermophilus in per ml seed liquors 5*108CFU), stir evenly, in 35-45 DEG C of fermentation 30d of temperature, fermentation terminated when pH reaches 4.3, obtains lactobacillus-fermented Liquid.

Step 3: fermenting bacillus natto.

By streptococcus acidi lactici fermented solution, 15% bafillus natto seed liquor is added(Contain natto gemma in per ml seed liquors Bacillus 4.5*107CFU), stir evenly, in 30-40 DEG C of fermentation 30d of temperature, obtain fermenting bacillus natto liquid.

Step 4: after-ripening.

Fermenting bacillus natto liquid is placed in refrigerated storage tank preservation 110d at 8-15 DEG C.

Step 5: filtering.

By the feed liquid after after-ripening, supernatant is extracted in first siphon, then is filtered through 0.22 μm of miillpore filter plate and frame filter press.

Step 6: ultra-high static pressure sterilizes.

Ultra-high static pressure sterilizes, and device therefor ultra-high static pressure pressure is 200Mpa, and processing time is that 40min is male to get liquid Red ferment.

It is prepared by 13 liquid tree peony ferment of embodiment.

Step 1: saccharomycetes to make fermentation.

By any tree peony zymotic fluids of embodiment 1-8,15% Hansenula anomala seed liquor is added(Per in ml seed liquors Contain Hansenula anomala 5*107CFU), stir evenly, in 25-35 DEG C of fermentation 15d of temperature, when the PH of fermentation system is reached Fermentation is terminated when 4.0, obtains saccharomycetes to make fermentation liquid.

Step 2: lactobacillus-fermented.

By saccharomycetes to make fermentation liquid, 5% Bifidobacterium seed liquid is added(Contain Bifidobacterium 5* in per ml seed liquors 108CFU), stir evenly, in 35-45 DEG C of 15 d of fermentation of temperature, fermentation terminated when pH reaches 4.3, obtains lactobacillus-fermented Liquid.

Step 3: fermenting bacillus natto.

By streptococcus acidi lactici fermented solution, 8% bafillus natto seed liquor is added(Contain natto gemma bar in per ml seed liquors Bacterium 4.5*107CFU), stir evenly, in 30-40 DEG C of fermentation 60d of temperature, obtain fermenting bacillus natto liquid.

Step 4: after-ripening.

Fermenting bacillus natto liquid is placed in refrigerated storage tank preservation 60d at 8-15 DEG C.

Step 5: filtering.

By the feed liquid after after-ripening, supernatant is extracted in first siphon, then is filtered through 0.22 μm of miillpore filter plate and frame filter press.

Step 6: ultra-high static pressure sterilizes.

Ultra-high static pressure sterilizes, and device therefor ultra-high static pressure pressure is 400Mpa, and processing time is that 20min is male to get liquid Red ferment.

It is prepared by 14 liquid tree peony ferment of embodiment.

Step 1: saccharomycetes to make fermentation.

By any tree peony zymotic fluids of embodiment 1-8,12% mixed yeast seed liquor, wherein compound barm strain is added In sub- liquid, Lu Shi Zygosaccharomyces bacterium and Weir saccharomycete volume ratio are 1:1(Contain Shandong per mL in Lu Shi Zygosaccharomyces bacterium seed liquors Family name's Zygosaccharomyces bacterium 5 × 107Cfu contains Weir saccharomycete 5 × 10 in Weir saccharomycete seed liquor per mL7cfu), stirring is It is even, in 25-35 DEG C of fermentation 10d of temperature, fermentation is terminated when the PH of fermentation system reaches 4.0, obtains saccharomycetes to make fermentation liquid.

Step 2: lactobacillus-fermented.

By saccharomycetes to make fermentation liquid, 8% mixing lactic acid bacteria seed liquor is added, wherein in mixing lactic acid bacteria seed liquor, protects and adds Leah lactobacillus and lactobacillus acidophilus volume ratio are 3:2(Contain bulgarian milk bar per mL in lactobacillus bulgaricus seed liquor Bacterium 5 × 108Cfu contains lactobacillus acidophilus 5 × 10 in lactobacillus acidophilus seed liquor per mL8cfu), stir evenly, in temperature 35-45 DEG C of fermentation 30d, fermentation is terminated when pH reaches 4.3, obtains streptococcus acidi lactici fermented solution.

Step 3: fermenting bacillus natto.

By streptococcus acidi lactici fermented solution, 15% bafillus natto seed liquor is added(Contain natto gemma in per ml seed liquors Bacillus 4.5*107CFU), stir evenly, in 30-40 DEG C of fermentation 30d of temperature, obtain fermenting bacillus natto liquid.

Step 4: after-ripening.

Fermenting bacillus natto liquid is placed in refrigerated storage tank preservation 110d at 8-15 DEG C.

Step 5: filtering.

By the feed liquid after after-ripening, supernatant is extracted in first siphon, then is filtered through 0.22 μm of miillpore filter plate and frame filter press.

Step 6: ultra-high static pressure sterilizes.

Ultra-high static pressure sterilizes, and device therefor ultra-high static pressure pressure is 200Mpa, and processing time is that 40min is male to get liquid Red ferment.

It is prepared by 15 solid tree peony ferment of embodiment.

By any liquid tree peony ferment of embodiment 9-14, concentrates by cryogenic vacuum, be freeze-dried at a temperature of -40 DEG C To solid tree peony ferment.

One, it in order to compare the nutriment difference of different strain fermentation tree peony ferment obtained, is sent out with the tree peony of embodiment 4 For zymotic fluid, we determine the Major Nutrients substance such as tree peony ferment albumen, total phenol, general flavone, total starches in each embodiment Content and tree peony ferment inoxidizability.

Use the tree peony ferment Major Nutrient substance and inoxidizability of different strain.

Index explanation.

1, gross protein value uses micro-Kjeldahl.

2, determining total phenol is sent out using forint colorimetric.

3, Determination of Total Flavonoids uses liquid chromatography.

4, total starches, which measure, uses anthrone colorimetry.

5, reducing sugar test uses DNS colorimetric methods.

6, inoxidizability detects its inoxidizability using DPPH methods using the clearance rate to free radical oxygen as index.

7, the tree peony ferment of embodiment 9 is using the tree peony zymotic fluid of embodiment 4 as raw material for example, i.e. embodiment 9(4) Sample be embodiment 4 prepared by tree peony zymotic fluid, other citing with reference to above-mentioned format.

The results show that same tree peony zymotic fluid, the synergistic bacterium that do not have to utilized is invented by us and is fermented, tree peony The Major Nutrients substances such as albumen, total phenol, general flavone, total starches in ferment have significant raising.Preferably, it is sent out in saccharomycete The ferment stage is compared with the embodiment 9,10,12,13 fermented using single culture, uses Lu Shi Zygosaccharomyces bacterium and Weir yeast The embodiment 11 of bacterium mixed fermentation, albumen, total phenol, general flavone, total starches, DPPH inhibiting rates in tree peony ferment are shown Work property improves(p<0.05);It in the lactobacillus-fermented stage, compares with the embodiment 9-13 to be fermented using single culture, is added using protecting The embodiment 14 of Leah lactobacillus and lactobacillus acidophilus mixed fermentation is albumen, total phenol, general flavone in tree peony ferment, total more Sugar, DPPH inhibiting rates have obtained conspicuousness raising(p<0.05).So the synergistic effect of a variety of bacterium is more advantageous in tree peony ferment The release of nutritional ingredient and active constituent, improves its nutrient utilization and inoxidizability, remains tree peony ferment to greatest extent Activity.

Two, in order to compare the nutriment difference of tree peony ferment made from different material, we determine each embodiment The contents of Major Nutrients substance such as middle tree peony ferment albumen, total phenol, general flavone, total starches and the inoxidizability of tree peony ferment.

The tree peony ferment Major Nutrient substance and inoxidizability of different material.

Index explanation.

1, in table the assay method of various parameters with reference to first part.

2, the tree peony ferment of embodiment 9 is respectively using the tree peony zymotic fluid of embodiment 1-8 as raw material for example, i.e. embodiment 9(1)Sample be embodiment 1 prepared by tree peony zymotic fluid, other citing with reference to above-mentioned format;Embodiment 15 [9 (1)] Solid tree peony ferment is the liquid tree peony ferment prepared with embodiment 9(It is illustrated as raw material using the tree peony zymotic fluid of embodiment 1 It is bright)For raw material for example, other citings press 1 with reference to above-mentioned format, by solid tree peony ferment:10 solid-liquid ratio adds sterile water-soluble Solution measures.

The results show that compared with common sea-buckthorn, the tree peony ferment that preparation method through the invention obtains contains The Major Nutrients substances such as a large amount of albumen, total phenol, general flavone, total starches, it is also higher to the clearance rate of DPPH free radicals, it is eating Product industry, health products and medicine and other fields have wide development prospect.

Three, in order to compare the effect of ultra-high static pressure sterilizing, by taking the tree peony zymotic fluid of embodiment 1 as an example, we determine The difference of common high-temperature sterilization nutritional ingredient and sense organ is replaced in embodiment 9.

Ultra-high static pressure sterilizes and the difference of high-temperature sterilization.

Index explanation.

1, in table the assay method of various parameters with reference to first part.

2, total number of bacteria is measured by the method for GB 4789.2-2010.

The results show that compared with high-temperature sterilization, ultra-high static pressure sterilizes under the premise of ensureing sterilization effect, quiet by superelevation The tree peony ferment that pressure sterilizing preparation method obtains, contains the Major Nutrients substance such as more total phenols, general flavone, total starches, sense organ Effect is more preferable, so, ultra-high static pressure sterilized more preferable keep tree peony ferment original local flavor, color and luster and nutritive value.

Claims (12)

1. a kind of preparation method of tree peony ferment, which is characterized in that the tree peony ferment be with peony or compounding moutan bark, One or both of peony meal is raw material, by material processing, low temperature sterilization, grinding mashing, fermentation, after-ripening, filtering, is surpassed Liquid tree peony ferment is prepared in high static pressure sterilizing, or obtains solid tree peony ferment using cryogenic vacuum concentration, freeze-drying Element, wherein the fermentation includes three saccharomycetes to make fermentation, lactobacillus-fermented, fermenting bacillus natto processes, is followed successively by
(1)Saccharomycetes to make fermentation process, the saccharomycete seed liquor of access tree peony slurries 10-15%, wherein containing ferment in per ml seed liquors Female bacterium 5 × 107CFU is stirred evenly, and in 25-35 DEG C of fermentation 10-15d of temperature, hair is terminated when the PH of fermentation system reaches 4.0 Ferment obtains saccharomycetes to make fermentation liquid;
(2)The lactobacillus solution of 5-10% is added in saccharomycetes to make fermentation liquid by lactic acid bacteria fermentation process, wherein per in ml seed liquors Containing lactic acid bacteria 5 × 108CFU is stirred evenly, and in 35-45 DEG C of fermentation 15-30d of temperature, fermentation is terminated when pH reaches 4.3, is obtained Streptococcus acidi lactici fermented solution;
(3)The Bacillus natto seed liquor of 8-15% is added in streptococcus acidi lactici fermented solution by fermenting bacillus natto process, wherein per in ml seed liquors Containing Bacillus natto 4.5 × 107CFU is stirred evenly, and in 30-40 DEG C of fermentation 30-60d of temperature, obtains fermenting bacillus natto liquid.
2. preparation method as described in claim 1, which is characterized in that the saccharomycete includes Hansenula anomala, wine brewing ferment It is one or more in female, Lu Shi Zygosaccharomyces and Weir yeast.
3. the preparation method as described in claim 1-2 is any, the lactic acid bacteria includes lactobacillus plantarum, lactobacillus fermenti, intestines It is one or more in film leukonid, streptococcus thermophilus, Bifidobacterium, lactobacillus bulgaricus and lactobacillus acidophilus.
4. the preparation method as described in claim 1-2 is any, the after-ripening are fermenting bacillus natto liquid being placed at 8-15 DEG C Refrigerated storage tank preservation 60-110d.
5. the preparation method as described in claim 1-2 is any, the ultra-high static pressure sterilizing, are device therefor ultra-high static pressure pressure Power is 200-400Mpa, processing time 20-40min.
6. the tree peony ferment that any preparation methods of claim 1-5 obtain.
7. tree peony ferment as claimed in claim 6, which is characterized in that the tree peony ferment is liquid tree peony ferment or solid Tree peony ferment.
8. tree peony ferment as claimed in claim 7, which is characterized in that the tree peony ferment is powder or on powder base It is further processed into particle, oral solution, tablet, capsule form.
9. a kind of product including any tree peony ferment of claim 6-8.
10. product as claimed in claim 9, which is characterized in that the product is food, health products or beautification product.
11. purposes of any tree peony ferment of claim 6-8 in preparing product.
12. purposes as claimed in claim 11, which is characterized in that the product is food, health products or beautification product.
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