CN109673842A - The feed addictive and preparation method thereof of nuisanceless low cost - Google Patents
The feed addictive and preparation method thereof of nuisanceless low cost Download PDFInfo
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- CN109673842A CN109673842A CN201910174225.1A CN201910174225A CN109673842A CN 109673842 A CN109673842 A CN 109673842A CN 201910174225 A CN201910174225 A CN 201910174225A CN 109673842 A CN109673842 A CN 109673842A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/10—Feeding-stuffs specially adapted for particular animals for ruminants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The present invention relates to a kind of feed addictives of nuisanceless low cost, comprising: 55-65 parts of corn, 10-15 parts of folium cortex eucommiae, 10-15 parts of Chinese holly leaf, 3-7 parts of apple pomace, 3-7 parts of wheat bran, 0.7-1.2 parts of calcium carbonate, 0.5-1 parts of bacillus subtilis, 0.5-1 parts of bacillus licheniformis, 0.5-1 parts of intestines streptococcus.The present invention also provides a kind of preparation methods of above-mentioned feed addictive, including (1) bacillus subtilis, bacillus licheniformis and the streptococcic preparation of intestines；(2) saccharification of corn flour and Eucommia Leaf Powder；(3) mixing of Chinese holly leaf powder, Eucommia Leaf Powder, apple pomace and wheatfeed；(4) material of (2) and (3) is mixed again, after mixing, the strain seed liquor prepared in inoculation (1), then unified fermentation to get.Feed addictive provided by the invention and preparation method thereof reduces the cost of production and cultivation and practical application effect more preferably, and operability is stronger, by the interaction of three kinds of probiotics, can effectively improve the immunity of poultry.
Description
Technical field
The invention belongs to feed additive fields, and in particular to the feed addictive of the nuisanceless low cost of one kind and its preparation
Method.
Background technique
Feed addictive refers to a small amount of or micro substance added in Feed Manufacturing processing, use process, in feed
Dosage is seldom but effect is significant.Feed addictive is the raw material that modern feed industry necessarily uses, to reinforcing basal feed nutrition
Value improves breeding performonce fo animals, guarantees animal health, saves feed cost, and improvement livestock products quality etc. has apparent
Effect.
Feed addictive is divided into additive for microbe feedstuff and Chinese herbal feed additive.Wherein, microbiological feed adds
Agent is a kind of microbial product replaced or balance one or more fungus strains in the animal ecosystem.It says in the narrow sense, it is a kind of
Own beneficial culture propagation can be excited to increase, while resisting the microbial product of harmful fungus strain growth.Additive for microbe feedstuff
Containing a large amount of beneficial bacterium (biodiasmin, Bifidobacterium, bacillus), complex enzyme, chelating peptide takes off mould dose etc., as feeding
It after material enters in livestock and poultry body, can breed rapidly, on the one hand, put into the metabolin of strain and enterogenous endotoxin inhibits other nocuousness
On the other hand the growth of flora forms normal microbial flora in host, synthesize main vitamin for host,
Nutrition is provided and prevents the invasion of pathogenic bacteria.
The harm of feed addictive antibiotic increasingly shows, and receives the whole world and widely pays close attention to, seek a kind of substitute and
The technology of substitution, the production efficiency that animal husbandry had not only been effectively ensured is unaffected, but also can reach the index of food safety is to be badly in need of grinding
Study carefully the shell of completion.The focus point of this respect current research, first is that about the probiotics and its metabolite that widely utilize；
Second is that digging office has potential material from China's Chinese medicine treasure-house abundant.
Summary of the invention
The present invention is directed to the limitation of single culture in practical applications, selects dead leaf bacillus, bacillus licheniformis
With intestines streptococcus, three kinds of probiotics, these three probiotics all have stronger acidproof alkali ability, and bacillus also has heat-resisting resistance to
Wet ability, three kinds of probiotics can smoothly enter into gastrointestinal tract under low ph value in animal body, and three kinds of probiotics have complementary advantages, cut
Maintenance poultry is played in fact and advocates colony balance, is conducive to the synergistic effect of probiotics, and dominant population may be implemented and biology is short of money
It is anti-to go out to enhance immune function.
To solve problems of the prior art, the present invention provides a kind of feed addictive of nuisanceless low cost, packet
Include each raw material component of following number: 55-65 parts of corn, 10-15 parts of folium cortex eucommiae, 10-15 parts of Chinese holly leaf, 3-7 parts of apple pomace, bran
3-7 parts of skin, 0.7-1.2 parts of calcium carbonate, 0.5-1 parts of bacillus subtilis, 0.5-1 parts of bacillus licheniformis, intestines hammer 0.5-1
Part.
Preferably, each raw material component including following number: 60 parts of corn, 13 parts of folium cortex eucommiae, 13 parts of Chinese holly leaf, apple
5 parts of slag, 5 parts of wheat bran, 1 part of calcium carbonate, 0.7 part of bacillus subtilis, 0.7 part of bacillus licheniformis, 0.7 part of intestines streptococcus.
The present invention also provides a kind of preparation methods of the feed addictive of nuisanceless low cost, include according to sequencing
Following steps:
Step (1): after folium cortex eucommiae is crushed plus water, progress soak at room temperature add water to carry out temperature control extraction, filter, obtain first
Filter residue plus water temperature control extraction again, filtering are obtained the second filtrate by filtrate and filter residue, after the first filtrate and the second filtrate are merged,
Obtain Cortex Eucommiae leaf extract；
Step (2): Cortex Eucommiae leaf extract obtained above is prepared into different fermentation mediums respectively, then by different
Fermentation medium prepares fermentation of bacillus subtilis seed liquor, the lichen bacillus ferments seed liquor and intestines Streptococcal fermentation respectively
Seed liquor；
Step (3): corn, folium cortex eucommiae, Chinese holly leaf, apple pomace, wheat bran are crushed into sieving for standby, the corn flour after taking sieving
Folium cortex eucommiae is successively gelatinized, is fluidized and is saccharified, folium cortex eucommiae, Chinese holly leaf, apple pomace, bran after adding calcium carbonate and sieving
Skin, mixing sterilize and cooling, are sequentially ingressed into bacillus subtilis made from step (2), bacillus licheniformis and intestines hammer strain
Sub- liquid simultaneously ferments；
Step (4): the fermentation material that step (3) is obtained carries out low temperature drying.
Preferably, the detailed method of operation of step (1) are as follows: add the water of 1 times of Cortex Eucommiae leaf quality after crushing folium cortex eucommiae, often
Temperature is impregnated 1 hour, and the water of 5 times of Cortex Eucommiae leaf qualities is added, and is extracted 40 minutes at 60-80 DEG C, and filtering obtains the first filtrate and filter residue, will
Filter residue adds the water of 2 times of Cortex Eucommiae leaf qualities, extracts 30 minutes at 60-80 DEG C, and filtering obtains the second filtrate, by the first filtrate and second
After filtrate merges, Cortex Eucommiae leaf extract is obtained.
In any of the above-described scheme preferably, in step (2), the system of the fermentation of bacillus subtilis seed liquor is prepared
Preparation Method is as follows: peptone 1-3%, beef extract 0.3-0.5%, sodium chloride 0.3-0.5%, and ammonium sulfate 0.03% is remaining to be
Cortex Eucommiae leaf extract 100ml, pH 6.8-7 are added in every 10L culture solution for water, according to volume very much be inoculated with than 5-10% it is withered
Careless bacillus, 37-40 DEG C of culture.
In any of the above-described scheme preferably, in step (2), the system of the lichen bacillus ferments seed liquor is prepared
Preparation Method is as follows: peptone 1-3%, beef extract 0.1-0.5%, and sodium chloride 0.1-0.5%, remaining is water, every 10L culture solution
Middle addition Cortex Eucommiae leaf extract 100ml, pH 7-7.2, carries out inoculation bacillus licheniformis, 37- than 5-10% very much according to volume
40 DEG C of cultures.
In any of the above-described scheme preferably, in step (2), the preparation side of the intestines Streptococcal fermentation seed liquor is prepared
Method is as follows: peptone 2-4%, beef extract 0.1-0.5%, glucose 2-4%, and calcium carbonate 0.5-1.5%, remaining is water, often
Cortex Eucommiae leaf extract 100ml, pH6.8-7 are added in 10L culture solution, carries out inoculation lichens gemma than 5-10% very much according to volume
Bacillus, 37-40 DEG C of culture.
In any of the above-described scheme preferably, in step (3), process is pulverized and sieved, selecting the sieve pore of sieve is 30 mesh.
Folium cortex eucommiae is a kind of Eucommiaceae plant, and alias silk cotton tree, silk flosssilk wadding skin, Yu Sipi have identical effective with Bark of Eucommia Ulmoides
Ingredient and pharmacological action.Source is richer, and cost is lower, has effects that nourishing liver and kidney, strengthening the bones and muscles and drop blood, and main component
Carbohydrate content is up to 50% or more, and protein content is 14.5% or so, also containing 15 kinds of microelements such as selenium, phosphorus, pressure
With preferable exploitation prospect.
Chinese holly leaf also known as carambola tree, have adaptable, and distribution is wide, easily breeds, and the short feature of germination period functionally has
There are kidney tonifying diuresis, strengthening the bones and muscles, protein content reaches 20-30%, also contains amino acid, vitamin, carbohydrate
With the ingredients such as microelement.
In apple pomace main component be soluble-carbohydrate, pectin, organic acid and cellulose in addition to fatty acid,
Part wheatfeed is supplemented simultaneously, contains higher carbohydrate and crude fibre in wheatfeed, is dead leaf bacillus, lichens bud
The fermentation of three kinds of probiotics of spore bacillus and intestines streptococcus provides sufficient energy.
In terms of feed addictive of nuisanceless low cost provided by the invention and preparation method thereof combines feed practical application
Pros and cons, reduce the cost of production and cultivation and practical application effect more preferably, operability is stronger, passes through three kinds of probiotics
Interaction, can effectively improve the immunity of poultry.
Specific embodiment
In order to be further understood that summary of the invention of the invention, the present invention is elaborated below in conjunction with specific embodiment.
Embodiment one
The present invention provides a kind of feed addictive of nuisanceless low cost, each raw material component including following number: corn
55 parts, 10 parts of folium cortex eucommiae, 10 parts of Chinese holly leaf, 3 parts of apple pomace, 3 parts of wheat bran, 0.7 part of calcium carbonate, 0.5 part of bacillus subtilis, ground
0.5 part of clothing bacillus, 0.5 part of intestines hammer, the strain of the above selection is the strain that can be bought on the market.
Embodiment two
The present invention provides a kind of feed addictive of nuisanceless low cost, each raw material component including following number: corn
58 parts, 11 parts of folium cortex eucommiae, 11 parts of Chinese holly leaf, 4 parts of apple pomace, 4 parts of wheat bran, 0.8 part of calcium carbonate, 0.6 part of bacillus subtilis, ground
0.6 part of clothing bacillus, 0.6 part of intestines hammer.
Embodiment three
The present invention provides a kind of feed addictive of nuisanceless low cost, each raw material component including following number: corn
60 parts, 13 parts of folium cortex eucommiae, 13 parts of Chinese holly leaf, 5 parts of apple pomace, 5 parts of wheat bran, 1 part of calcium carbonate, 0.7 part of bacillus subtilis, lichens
0.7 part of bacillus, 0.7 part of intestines streptococcus.
Example IV
The present invention provides a kind of feed addictive of nuisanceless low cost, each raw material component including following number: corn
63 parts, 14 parts of folium cortex eucommiae, 14 parts of Chinese holly leaf, 6 parts of apple pomace, 6 parts of wheat bran, 1.1 parts of calcium carbonate, 0.9 part of bacillus subtilis, ground
0.9 part of clothing bacillus, 0.9 part of intestines hammer.
Embodiment five
The present invention provides a kind of feed addictive of nuisanceless low cost, each raw material component including following number: corn
65 parts, 15 parts of folium cortex eucommiae, 15 parts of Chinese holly leaf, 7 parts of apple pomace, 7 parts of wheat bran, 1.2 parts of calcium carbonate, 1 part of bacillus subtilis, lichens
1 part of bacillus, 1 part of intestines hammer.
The preparation of fermentation of bacillus subtilis seed liquor
Qualified fresh folium cortex eucommiae is weighed into 1kg, 1L water is first added after crushing, soak at room temperature 1 hour, adds 5L water, into
The extraction of row first time temperature control, at 60-80 DEG C, extraction time 40min, extraction fluid mends 2L water again for temperature control, carries out the
Secondary extraction, temperature are controlled at 60-80 DEG C, and extraction 30min does not merge extracting solution twice, are refrigerated spare.
Peptone 90g, beef extract 22.5g, sodium chloride 15g are weighed, ammonium sulfate 1.5g is placed in the water of 1000ml, is added
The Cortex Eucommiae leaf extract of 35ml dissolves by heating, and adjusts pH to 6.8-7.0 with 20%NaOH and 2mol/L HCL, is supplemented with pure water
To 3000ml.
The above-mentioned culture medium prepared is sub-packed in the test tube of 15*150mm (every 9ml), (every bottle of 250ml triangular flask
175ml), 3000ml round-bottomed flask (every bottle of 1000ml), bandages and is placed in electrothermal sterile device, 115-121 DEG C of sterilizing 25min,
Cooling is spare.
The inclined-plane bacillus subtilis strain of production is taken to transfer in bacillus subtilis level-one test tube, 30 DEG C of cultures 22 are small
When, then take the one grade fermemtation liquid fermented to be inoculated into the triangular flask of the second level culture solution containing 175ml by 5% inoculum concentration
In, 40 DEG C are cultivated 22 hours, in transfer the in time round-bottomed flask of above-mentioned configured 1-10L and the fermentor of 30L stainless steel.
Peptone 200-600g, beef extract 50-100g, sodium chloride 30-100g are weighed, ammonium sulfate 3-10g will now be weighed
Peptone, beef extract, sodium chloride and ammonium sulfate be placed in the container or stainless steel fermentation tank of 1-6L, dissolve by heating, with 20%
NaOH and 2mol/L HCL adjust pH to 7.0 after, move in round-bottomed flask or 30L stainless steel fermentation tank in, add water to be supplemented to 1L
Or 10L, fermentor feeding port is jumped a queue or closes, round-bottomed flask is placed in electrothermal sterile device, and stainless steel fermentation tank is opened sterilizing and risen
Temperature controls 115-121 DEG C of sterilising temp, sterilization time 15-25min, after being cooled to 37-40 DEG C, the switching of flame inoculation method respectively
Three grade fermemtation liquid 175ml or 1L are spaced 1 hour shaking flask or open the agitating device in stainless steel fermentation tank, shake up or stir 5-
10min, 37 ± 2 DEG C are cultivated 22 hours to get fermentation of bacillus subtilis seed liquor.
The preparation of the lichen bacillus ferments seed liquor
Peptone 90g, beef extract 15g are weighed, sodium chloride 15g is placed in the water of 1000ml, and the folium cortex eucommiae that 35ml is added mentions
Liquid is taken, is dissolved by heating, pH to 7.0 is adjusted with 20%NaOH and 2mol/L HCL, is supplemented to 3000ml with pure water.
The above-mentioned culture medium prepared is sub-packed in the test tube of 15*150mm (every 9ml), (every bottle of 250ml triangular flask
175ml), 3000ml round-bottomed flask (every bottle of 1000ml), bandages and is placed in electrothermal sterile device, 118-121 DEG C of sterilizing 25-
28min, cooling are spare.
The inclined-plane bacillus licheniformis kind of production is taken to transfer in bacillus licheniformis level-one test tube, 37 ± 2 DEG C of cultures
18-22 hours, the one grade fermemtation liquid fermented is then taken to be inoculated into the second level culture solution containing 175ml by the inoculum concentration of 5-8%
Triangular flask in, 37 ± 2 DEG C cultivate 22 hours, then take the second order fermentation liquid fermented to be inoculated in by 5-8% inoculum concentration
In the three-level round-bottomed flask of 1000ml, 37-40 DEG C culture 18-22 hours.
Peptone 300g, beef extract 50g are weighed, sodium chloride 30g is placed in 6L pure water, is dissolved by heating, is used 20%NaOH
It after adjusting pH to 7.0 with 2mol/L HCL, is transferred completely into 30L stainless steel fermentation tank, water is added to be supplemented to 10L, close fermentation
Tank feeding port, setting stainless steel fermentation tank open sterilizing heating, control 118-121 DEG C of sterilising temp respectively, sterilization time 25-
30min, after being rapidly cooled to 37 ± 2 DEG C, flame inoculation method switching three grade fermemtation liquid 1L, interval is shaken for 1 hour opens stainless steel hair
Agitating device in fermentation tank stirs 5-10min, and 37 ± 2 DEG C are cultivated 22 hours to get the lichen bacillus ferments seed liquor.
The preparation of intestines Streptococcal fermentation seed liquor
Peptone 180g, beef extract 15g, sodium chloride 15g, 15g, 1-2 grades of calcium carbonate are used lactose 60g, and 3 grades are used glucose
120g will be placed in the water of 1000ml at good peptone, beef extract, sodium chloride, the Cortex Eucommiae leaf extract of 35ml be added, adds
Lactose is added in heat of solution, adjusts pH to 6.5-6.8 with 20%NaOH and 2mol/L HCL, is supplemented to 3000ml with pure water.
The above-mentioned culture medium prepared is sub-packed in the test tube of 15*150mm (every 9ml), (every bottle of 250ml triangular flask
175ml), 10g calcium carbonate and 40g glucose are added in round-bottomed flask, bandages and sets by 3000ml round-bottomed flask (every bottle of 1000ml)
In electrothermal sterile device, 118-121 DEG C of sterilizing 25-30min is cooling spare.
Take the inclined-plane intestines hammer strain transfer of production in intestines streptococcus level-one test tube and second level triangular flask culture medium,
37-39 DEG C culture 18-22 hours, then take the second level triangular flask fermentation liquid fermented by the inoculum concentration of 5-6% be inoculated into containing
In the round-bottomed flask of 1000ml culture medium, 37-39 DEG C culture 18-22 hours.
Peptone 370g, beef extract 50g, sodium chloride 30g, calcium carbonate 100g, glucose 400g are weighed, it successively will be at good
Peptone, beef extract, sodium chloride be placed in 6L pure water, dissolve by heating, with 20%NaOH and 2mol/L HCL adjust pH to
After 6.8-7.0, calcium carbonate 100g is added, glucose 400g is transferred completely into 30L stainless steel fermentation tank, water is added to be supplemented to
10L closes fermentor feeding port, and setting stainless steel fermentation tank opens sterilizing heating, controls 115-120 DEG C of sterilising temp respectively,
Sterilization time 25-30min, after being cooled to 37-40 DEG C, flame inoculation method switching three grade fermemtation liquid 1L, interval shakes unlatching for 1 hour not
The agitating device to become rusty in steel fermentor stirs 5-10min, and 37-40 DEG C is cultivated 22 hours to get intestines Streptococcal fermentation seed liquor.
The preparation of solid fermentation culture medium and additive
Divide 30 mesh of tunnel spare the removal of impurities of the corn of formulation dosage, folium cortex eucommiae, Chinese holly leaf, apple pomace and wheat bran respectively.
The corn flour 1.8kg of above-mentioned processing qualification is weighed respectively, and Eucommia Leaf Powder 300g is placed in rustless steel container, is added
5kg pure water, is made slurry, is heated to 80 DEG C, is gelatinized 25min, and amylase is added in the slurry pH to 6.8 after adjusting gelatinization
3.6g controls 76 DEG C of stream temperature, is detected with dilute iodine solution, and it is to fluidize to terminate that fluidisation liquid, which becomes dilute iodine solution primary colors from blue,；It opens
Dynamic stirring, the slurry tune pH to 5.0 that will have been fluidized control temperature 50 C, are slowly added to carbohydrase 3.5g, and heat preservation is saccharified 5 hours,
After saccharification, calcium carbonate 30g, Eucommia Leaf Powder 150g, Chinese holly leaf powder 450g, apple pomace 150g, wheatfeed 150g, adjustment is added
Mixed material water content is 30%-40%, and adjustment pH is 7.0-7.2, after mixing, sets 118-121 DEG C of sterilising temp, goes out
Bacterium time 28-30min, is rapidly cooled to 37-40 DEG C, then is sequentially ingressed into uninteresting bacillus seed liquor, and fermentation accesses after 8 hours
Bacillus licheniformis seed liquor accesses intestines streptococcus seed liquor after fermentation 8 hours, continues fermentation 18-22 hours, when whole process is fermented
Between control at 42-48 hour, primary every 4 hours starting solid fermentation electric stirrings in solid fermentation whole process, whole-process control is sent out
Ferment temperature is 38-40 DEG C；The good fermentation materials of solid fermentation are transferred in preheated drying machine, drying temperature, air inlet are controlled
It is 50-60 DEG C of temperature, 40-44 DEG C of leaving air temp, about 4 hours dry, up to feed addictive provided by the invention after having dried.
Product content and effect
Every gram of final products contains the uninteresting bacillus at least 200,000,000,000 of activity, and every gram contains activity at least 100,000,000,000, and every gram contains
Active intestines streptococcus at least 200,000,000,000, contain mannatide >=200mg/kg, contain chlorogenic acid >=0.4%, Geniposidic acid >=
0.4%, general flavone >=0.4% contains total reducing sugar >=12%, contains protein >=1.2%.
The dosage of feed addictive provided by the invention is the 1-3% of normal feeding feed stuff amount, applies illustration: intestines with milk cow
Tract disease declines 50-70%, and Milk Production improves 15-30%, and protein content improves 4.32% than control group in milk, blood with
And the harmful substance in milk is not detected.
It will be apparent to those skilled in the art that feed addictive of nuisanceless low cost of the invention and preparation method thereof wraps
Include the summary of the invention and specific embodiment part of aforementioned present invention specification, as space is limited and for keep specification concise without
Each scheme that these combinations are constituted is described one by one.All within the spirits and principles of the present invention, any modification for being made, equivalent
Replacement, improvement etc., should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of feed addictive of nuisanceless low cost, each raw material component including following number: 55-65 parts of corn, Cortex Eucommiae
10-15 parts of leaf, 10-15 parts of Chinese holly leaf, 3-7 parts of apple pomace, 3-7 parts of wheat bran, 0.7-1.2 parts of calcium carbonate, bacillus subtilis
0.5-1 parts, 0.5-1 parts of bacillus licheniformis, 0.5-1 parts of intestines hammer.
2. the feed addictive of nuisanceless low cost according to claim 1, which is characterized in that including each of following number
Raw material components: 60 parts of corn, 13 parts of folium cortex eucommiae, 13 parts of Chinese holly leaf, 5 parts of apple pomace, 5 parts of wheat bran, 1 part of calcium carbonate, withered grass gemma
0.7 part of bacillus, 0.7 part of bacillus licheniformis, 0.7 part of intestines streptococcus.
3. a kind of preparation method of the feed addictive of nuisanceless low cost, according to sequencing the following steps are included:
Step (1): after folium cortex eucommiae is crushed plus water, progress soak at room temperature add water to carry out temperature control extraction, filter, obtain the first filtrate
And filter residue, filter residue plus water temperature control extraction again, filtering are obtained into the second filtrate, after the first filtrate and the second filtrate are merged, must be shut out
Secondary leaf extract；
Step (2): Cortex Eucommiae leaf extract obtained above is prepared into different fermentation mediums respectively, then passes through different fermentations
Culture medium prepares fermentation of bacillus subtilis seed liquor, the lichen bacillus ferments seed liquor and intestines Streptococcal fermentation seed respectively
Liquid；
Step (3): corn, folium cortex eucommiae, Chinese holly leaf, apple pomace, wheat bran are crushed into sieving for standby, the corn flour Cortex Eucommiae after taking sieving
Leaf is successively gelatinized, is fluidized and is saccharified, and folium cortex eucommiae, Chinese holly leaf, apple pomace, wheat bran after adding calcium carbonate and sieving mix
It closes, sterilizes and cooling, be sequentially ingressed into bacillus subtilis made from step (2), bacillus licheniformis and intestines streptococcus seed liquor
And it ferments；
Step (4): the fermentation material that step (3) is obtained carries out low temperature drying.
4. the preparation method of the feed addictive of nuisanceless low cost according to claim 3, which is characterized in that step
(1) detailed method of operation are as follows: the water for adding 1 times of Cortex Eucommiae leaf quality after crushing folium cortex eucommiae soak at room temperature 1 hour, adds 5 times of Cortex Eucommiaes
The water of leaf quality extracts 40 minutes at 60-80 DEG C, and filtering obtains the first filtrate and filter residue, by filter residue plus 2 times of Cortex Eucommiae leaf qualities
Water extracts 30 minutes at 60-80 DEG C, and filtering obtains the second filtrate, after the first filtrate and the second filtrate are merged, obtains folium cortex eucommiae and mentions
Take liquid.
5. the preparation method of the feed addictive of nuisanceless low cost according to claim 3, which is characterized in that step
(2) in, prepare the fermentation of bacillus subtilis seed liquor the preparation method is as follows: peptone 1-3%, beef extract 0.3-
0.5%, sodium chloride 0.3-0.5%, ammonium sulfate 0.03%, remaining is water, and Cortex Eucommiae leaf extract is added in every 10L culture solution
100ml, pH 6.8-7 carry out inoculation bacillus subtilis, 37-40 DEG C of culture than 5-10% very much according to volume.
6. the preparation method of the feed addictive of nuisanceless low cost according to claim 3, which is characterized in that step
(2) in, prepare the lichen bacillus ferments seed liquor the preparation method is as follows: peptone 1-3%, beef extract 0.1-
0.5%, sodium chloride 0.1-0.5%, remaining is water, and Cortex Eucommiae leaf extract 100ml, pH 7-7.2 is added in every 10L culture solution,
Inoculation bacillus licheniformis, 37-40 DEG C of culture are carried out than 5-10% very much according to volume.
7. the preparation method of the feed addictive of nuisanceless low cost according to claim 3, which is characterized in that step
(2) in, prepare the intestines Streptococcal fermentation seed liquor the preparation method is as follows: peptone 2-4%, beef extract 0.1-0.5%, Portugal
Grape sugar 2-4%, calcium carbonate 0.5-1.5%, remaining is water, and Cortex Eucommiae leaf extract 100ml is added in every 10L culture solution,
PH6.8-7 carries out inoculation bacillus licheniformis, 37-40 DEG C of culture than 5-10% very much according to volume.
8. the preparation method of the feed addictive of nuisanceless low cost according to claim 3, which is characterized in that step
(3) in, process is pulverized and sieved, selecting the sieve pore of sieve is 30 mesh.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910174225.1A CN109673842A (en) | 2019-03-08 | 2019-03-08 | The feed addictive and preparation method thereof of nuisanceless low cost |
Applications Claiming Priority (1)
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| CN113229403A (en) * | 2021-06-02 | 2021-08-10 | 杨春凯 | Animal enzyme and preparation process thereof |
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