CN109673751A - A kind of preparation method and application of fruits and vegetables natural polypeptides combination composition chewable tablet - Google Patents
A kind of preparation method and application of fruits and vegetables natural polypeptides combination composition chewable tablet Download PDFInfo
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- CN109673751A CN109673751A CN201910086710.3A CN201910086710A CN109673751A CN 109673751 A CN109673751 A CN 109673751A CN 201910086710 A CN201910086710 A CN 201910086710A CN 109673751 A CN109673751 A CN 109673751A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/1526—Amino acids; Peptides; Protein hydrolysates; Nucleic acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
- A23C9/156—Flavoured milk preparations ; Addition of fruits, vegetables, sugars, sugar alcohols or sweeteners
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/18—Milk in dried and compressed or semi-solid form
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/16—Vegetable proteins from soybean
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/20—Proteins from microorganisms or unicellular algae
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
Abstract
The present invention provides a kind of fruits and vegetables natural polypeptides combination composition chewable tablets and preparation method thereof, belong to functional food field.The component of chewable tablets fruits and vegetables natural biological polypeptide Gly-His-Lys containing there are many, add pure red date powder and milk powder.Preparation method is: a variety of fruits and vegetables natural biological polypeptide powder, pure red date powder and the milk powder after above-mentioned sieving being carried out mixing by weighing according to a certain ratio, are directly compressed into fruits and vegetables natural polypeptides combination composition chewable tablet by tablet press machine after mixing.The chewable tablets form is complete, and smooth appearance, hardness is moderate, good in taste, instant edible, takes this product by chewing, active constituent can be directly scattered in oral cavity and alimentary canal, can quickly be absorbed.Fruits and vegetables natural polypeptides combination composition chewable tablet provided by the invention preferably maintains the physiological activity of fruits and vegetables natural polypeptides, can be used for preparing health food and food uses.
Description
Technical field
The invention belongs to the technical fields of functional food, and in particular to a kind of fruits and vegetables natural polypeptides combination composition chewable tablet and its
Preparation method.
Background technique
Peptides are divided into oligopeptides and polypeptide, are one kind biologies by two or more amino acid condensations and connected with peptide bond
Active material.Peptide is the structure and function segment for constituting protein, and molecular weight is usually called small point in the peptide of 180-10000Da
Sub- active peptide, compared with other materials in organism, maximum feature is that they have very high bioactivity and diversity.
In vivo, many bioactive substances be mostly exist with the molecular structure of polypeptide and native conformation, while also with
The mode of action of the natural bioactive effect important in physical exertion.Growth and development and metabolism of the polypeptide in organism
In play a crucial role.Active bio polypeptide can be with the vital movement of regulating cell to ensure cell proper splitting and egg
White matter normally synthesizes, therefore plays an important role to cell division, differentiation, reparation.Therefore active bio polypeptide and human health, anti-
Aging and promote longevity etc. closely related and significant.Scientific research confirmation in recent years, the master of the absorbed protein of human body
Wanting form is not all amino acid, and 70% or more is that therefore can pass through supplement active bio polypeptide in the form of active bio peptide
Mode improves health, resists the disease and aging.
There are many extraction preparation method of polypeptide at present, including acid-hydrolysis method, alkali hydrolysis method, enzyme hydrolysis method and several method
The methods of connected applications.However, retaining the extraction of the polypeptide of natural activity, i.e., in the structure and property for not changing these compounds
Under conditions of, the impurity in extract is separated as far as possible, needs more mild extraction conditions.Simple acidolysis or alkaline hydrolysis
And enzymatic isolation method extracts polypeptide, if selected solution system is acid or alkalinity is too strong, extract may all occurred irreversible
Denaturation or excessively hydrolysis and molecular conformation etc. are substantially change, it is made gradually to lose the biological activity of polypeptide and function
Energy.Moreover, in order to which the protease for preventing enzymatic isolation method from being added after completing polypeptide and extracting continues generation hydrolysis, it is generally necessary to boil
It is living to carry out enzyme deactivation, the reduction or destruction of polypeptide bioactivity may be caused to a certain extent in this way.
It is important that the above method is all to realize to obtain polypeptide by decomposition of protein, and it is generally complex for operation step, draw
Enter that medium is more, there are more problems for the bioactivity of gained polypeptide.Process provides one kind can be from biological tissue directly
The easy polypeptide production methods for extracting natural polypeptides are connect, this method extraction conditions are mild, and separation condition takes physiological condition, institute
Its natural activity can be retained by obtaining polypeptide.
It is straight after mixing that raw material can be achieved in the fruits and vegetables natural polypeptides combination composition chewable tablet preparation method that this method provides
It connects by tabletting machine, had both avoided existing wet granulation and spraying granulation humidifies in the process and high temperature drying causes later
Polypeptide active it is impaired, while also simplifying the operating procedure of tabletting, reduce added in Conventional compression manufacturing process it is various
Auxiliary material.
The fruits and vegetables natural polypeptides composition that this method provides is derived from natural fruit and vegetables, including fructus lycii, sweet potato leaves, hardship
Melon, peony seeds, Loquat-Shaped Cake, yacon, oat, onion, purple perilla, tea seed, semen sojae atricolor, pumpkin flower, walnut, black fungus, peanut, flue
Fruit, golden Rosa roxburghii Tratt, blueberry, spirulina, grape, sea-buckthorn, Chinese yam, pollen pini, wild Fructus actinidiae chinensis, taro, broccoli etc., these fruits and vegetables
In the organic matters such as protein rich in, lipid, carbohydrate, while there are also various vitamin and minerals.Many natural fruit and vegetables
Have medical efficacy, food therapy value and nutritive value abundant, to human health is kept, prevents and cures diseases and play an important role.
Eat more these natural fruit and vegetables can with stomach strengthening and digestion promoting, nourishing and fit keeping function, help sleep, it is antifatigue, help dispelling toxicity, detoxicating, prevent old bone
Matter is loose, improve myocardial function, prevention and treatment heart disease, can with the beauty of Eradicates spot, anti-aging, can be with cancer-resisting, strengthen immunity, energy
Enough hypoglycemic, Adjust-blood lipid, blood pressure lowering, eliminating dampness and heat, the microcirculation for the blood vessel that scattered stasis detumescence, boosts metabolism, improves etc., therefore
There is essential effect to delaying human body caducity and health care.
The fruits and vegetables natural biological polypeptide of this method providing method preparation is not required to disappear due to its special Physiology and biochemistry property
Change, is easily quickly completely directly absorbed and utilized by human body, the adverse reactions such as diarrhea will not be caused after food；Fruits and vegetables are naturally given birth to simultaneously
Object active peptide has again compared with low antigenicity, will not cause allergic reaction；Active peptides can participate in vivo protein in human body
Synthesis promotes mitosis and the Proliferation, Differentiation of relevant cell, enhances the metabolism of body tissue and cell, therefore have
Regulate and control cell metabolism on molecular level, the effect repaired abnormal damaged cell, improve cell metabolism.Fruits and vegetables natural activity peptide
In addition to directly have outside antioxidant activity can also active cell it is active, effectively remove the free radical being harmful to the human body.Natural activity
Peptide can be directly entered cell and cell membrane fusion, the invasion of bacterium and virus be prevented, to play antiviral, antibacterial work
With enhancing the activity of immunocyte.Small molecule natural activity peptide can promote the conjunction of collagen and elastin laminin on the skin
At the effect of promoting the proliferation of cell, therefore beautifying face and moistering lotion can be played.Furthermore natural biological polypeptide also has potential drop blood
Pressure, reducing blood lipid, reduction cholesterol, promotion mineral absorption, improvement metabolism, enhancing body immunity, strong physical strength etc.
Function.The cell that fruits and vegetables natural biological polypeptide can activate rest cell, repair defective cell, regeneration necrosis, therefore fruits and vegetables day
Right active peptides are capable of the health of Effective Regulation human body on a cellular level, resist aging and disease.
Summary of the invention
The present invention provides a kind of fruits and vegetables natural polypeptides combination composition chewable tablets and preparation method thereof.
The technical solution adopted by the invention is as follows:
A kind of fruits and vegetables natural polypeptides combination composition chewable tablet and preparation method thereof, is made of the raw material of following mass fraction:
8-20 parts of fruits and vegetables natural polypeptides Gly-His-Lys,
Pure red date powder 45-80 parts,
12-32 parts of milk powder；
The preparation method of above-mentioned fruits and vegetables natural polypeptides combination composition chewable tablet carries out as steps described below:
(1) fruits and vegetables natural polypeptides Gly-His-Lys are prepared:
A. fresh fruit of vegetables tissue is cleaned, is diced, is homogenized under 2-8 DEG C of ice bath, precooled fruits and vegetables tissue weight 1-3 is then added
The phosphate buffer of 0.02-0.2 mol/L pH6.0-7.2 again impregnates, and after being placed in 2-8 DEG C of refrigerator 12 hours, 2-8 DEG C cold
Freeze centrifugation, supernatant is the crude extract containing fruits and vegetables natural polypeptides；
B. eluent is added into the crude extract containing fruits and vegetables natural polypeptides obtained by step a and is diluted most 30-100 containing polypeptide
The solution of mg/ml, is separated with sephadex chromatography, collects the component of molecular weight 500-12000 dalton；
C. the fruits and vegetables natural polypeptides collection liquid vacuum freeze drying in step b is lyophilized to get to fruits and vegetables natural polypeptides extract
Powder；
(2) it crushes: jujube is cut to drying and crushing after doing stoning；
(3) it is sieved: gained fruits and vegetables natural polypeptides freeze-dried powder, pure red date powder and milk powder in step (1) is crossed into -100 mesh of 60 mesh；
(4) mixing: natural polypeptides Gly-His-Lys, pure red date powder, the milk powder after sieving are uniformly mixed by above-mentioned mass fraction；
(5) uniformly mixed raw material tabletting: is obtained into chewable tablets finished product through tablet press machine direct tablet compressing.
Preferably, above-mentioned fruits and vegetables natural polypeptides combination composition chewable tablet is made of the raw material of following mass fraction:
12.5 parts of fruits and vegetables natural polypeptides Gly-His-Lys,
67.5 parts of pure red date powder,
20 parts of milk powder.
Preferably, above-mentioned fruits and vegetables natural polypeptides combination composition chewable tablet and preparation method thereof, wherein fruits and vegetables natural polypeptides is selected from
Fructus lycii polypeptide, sweet potato leaves polypeptide, bitter gourd polypeptide, peony seeds polypeptide, Loquat-Shaped Cake polypeptide, yacon polypeptide, oat polypeptide, onion are more
Peptide, purple perilla polypeptide, tea-seed peptide, semen sojae atricolor polypeptide, pumpkin flower polypeptide, walnut polypeptide, black fungus polypeptide, Peanut Polypeptide, dragon fruit
Polypeptide, golden Rosa roxburghii Tratt polypeptide, blueberry polypeptide, spirulina polypeptide, Grape Skin polypeptide, sea-buckthorn polypeptide, Chinese yam polypeptide, preserved egg powder polypeptide,
A variety of mixtures in the types such as wild Fructus actinidiae chinensis polypeptide, taro polypeptide, broccoli polypeptide.
Preferably, above-mentioned fruits and vegetables natural polypeptides combines the preparation method of composition chewable tablet, wherein phosphate described in step (1)
Buffer concentration is 0.05 mol/L, and the phosphate buffer pH is 7.0, and the buffer dosage is the 2 of fruits and vegetables tissue
Times, it is 50 mg/mls that polypeptide solution is contained after the dilution.
Preferably, above-mentioned fruits and vegetables natural polypeptides combines the preparation method of composition chewable tablet, wherein day described in above-mentioned steps (3)
Right polypeptide freeze-dried powder, pure red date powder and milk powder cross 80 meshes.
Fruits and vegetables natural polypeptides combination composition chewable tablet provided by the invention and preparation method thereof is by being added pure red date powder and cream
Powder, the characteristic using red date powder itself containing polysaccharide can be realized is directly over tabletting machine after mixing, has both avoided existing
It is humidified during some wet granulations and spraying granulation and polypeptide active caused by high temperature drying is impaired later, also simplified simultaneously
The operation of tabletting.
Fruits and vegetables natural polypeptides combination composition chewable tablet preparation method provided by the invention is easy to operate, can be from biological tissue
Natural biological polypeptide is directly extracted, this method extraction conditions are mild, and separation condition takes physiological condition, and gained polypeptide can retain
Its natural bioactive retains higher physiological activity with the combination composition chewable tablet of fruits and vegetables natural polypeptides obtained by this method, can be with
The application being used to prepare in health food and food.
The fruits and vegetables natural polypeptides combination composition chewable tablet form of the method provided by the present invention preparation is complete, smooth appearance, and hardness is suitable
In, it is good in taste, any compression forms of instant edible can be fabricated to, taking this product its active constituent by chewing can be direct
It is scattered in oral cavity and alimentary canal, can quickly absorb, the higher physiological activity retained is so as in cellular level
The health of upper Effective Regulation human body resists aging and disease.
Detailed description of the invention
Fig. 1 fruits and vegetables natural polypeptides combines composition chewable tablet circular piece；
Fig. 2 fruits and vegetables natural polypeptides combines composition chewable tablet triangular piece；
Fig. 3 fruits and vegetables natural polypeptides combines composition chewable tablet ellipse slice；
Maintenance effect diagram of Fig. 4 fruits and vegetables natural polypeptides composition to the mice burden swimming time.
Specific embodiment is only that the present invention is a kind of for further illustrating but being not limited to the present invention, following example below
Embodiment.
Specific embodiment
A kind of preparation method of the sweet potato leaves natural biological polypeptide extract of embodiment 1, steps are as follows:
(1) sweet potato leaves are taken, are diced, are homogenized under 4 DEG C of ice baths, the 0.05mol/L of 2 times of precooled sweet potato leaves weight is then added
The phosphate buffer of pH7.0 impregnates, and is placed in 4 DEG C of refrigerators after 12 hours, 4 DEG C of refrigerated centrifuges, and supernatant is to contain sweet potato leaves day
Right polypeptide crude extract；
(2) eluent is slowly added into the crude extract containing sweet potato leaves polypeptide obtained by step (1) be diluted to 50mg/ containing polypeptide
The solution of mL, with sephadex chromatography (Sephadex G-50 column, internal diameter 1cm, column length 60cm, eluent 0.05mol/L,
The phosphate buffer of pH7.0, sodium chloride solution containing 0.15mol/L, flow velocity 2mL/min, sample volume 1mL, Detection wavelength
It 280nm) separates, collects the component of molecular weight 500-12000D；
(3) the sweet potato leaves natural polypeptides collection liquid freeze-drying in step (2) is frozen to get to sweet potato leaves active polypeptide extract
Dry powder.
The preparation of more than 2 kinds of fruits and vegetables natural polypeptides of embodiment
Fructus lycii polypeptide, bitter gourd polypeptide, peony seeds polypeptide, Loquat-Shaped Cake polypeptide, yacon are extracted respectively by the operating procedure of embodiment 1
Polypeptide, oat polypeptide, onion polypeptide, purple perilla polypeptide, tea-seed peptide, semen sojae atricolor polypeptide, pumpkin flower polypeptide, walnut polypeptide, black fungus
Polypeptide, Peanut Polypeptide, dragon fruit polypeptide, golden Rosa roxburghii Tratt polypeptide, blueberry polypeptide, spirulina polypeptide, Grape Skin polypeptide, sea-buckthorn polypeptide,
Chinese yam polypeptide, preserved egg powder polypeptide, wild Fructus actinidiae chinensis polypeptide, taro polypeptide, broccoli polypeptide, for use.
A kind of preparation method of the fruits and vegetables natural polypeptides of embodiment 3 combination composition chewable tablet:
(1) by the natural Gly-His-Lys of fruits and vegetables, jujube, milk powder, 60 meshes be crushed respectively；
(2) fructus lycii polypeptide 1.5g, sweet potato leaves polypeptide 1.5g, peony seeds polypeptide 2.5g, yacon polypeptide 1.4g, oat are successively weighed
Polypeptide 3.8g, purple perilla polypeptide 1.8g, pure red date powder 67.5g, milk powder 20g are uniformly mixed；
(3) uniformly mixed raw material tabletting: is obtained into the round chewable tablets finished product that diameter is 2.5cm through tablet press machine direct tablet compressing.
A kind of preparation method of the fruits and vegetables natural polypeptides of embodiment 4 combination composition chewable tablet:
(1) by the natural Gly-His-Lys of fruits and vegetables, jujube, milk powder, 60 meshes be crushed respectively；
(2) tea-seed peptide 1.1g, semen sojae atricolor polypeptide 0.8g, pumpkin flower polypeptide 1.2g, sea-buckthorn polypeptide 1.5g, Chinese yam polypeptide are successively weighed
0.8g, broccoli polypeptide 1.3g, yacon polypeptide 1.8g, oat polypeptide 2.2g, wild Fructus actinidiae chinensis polypeptide 1.3g, pure red date powder
70g, milk powder 18g are uniformly mixed；
(3) tabletting: the round chewable tablets finished product that diameter is 2.5cm is obtained with single-punch tablet press tabletting.
A kind of preparation method of the fruits and vegetables natural polypeptides of embodiment 5 combination composition chewable tablet:
(1) it by the natural Gly-His-Lys of fruits and vegetables, jujube, milk powder, crushes sieve with 100 mesh sieve respectively；
(2) fructus lycii polypeptide 1.0g is successively weighed, walnut polypeptide 0.5g, black fungus polypeptide 1.2g, bitter gourd polypeptide 0.4g, broccoli is more
Peptide 0.6g, peony seeds polypeptide 2.0g, preserved egg powder polypeptide 1.3g, taro polypeptide 1.0g, pure red date powder 80g, milk powder 12g, mixing are equal
It is even；
(3) tabletting: the triangle chewable tablets finished product that side length is 2.1cm is obtained with single-punch tablet press tabletting.
A kind of preparation method of the fruits and vegetables natural polypeptides of embodiment 6 combination composition chewable tablet:
(1) by the natural Gly-His-Lys of fruits and vegetables, jujube, milk powder, 80 meshes be crushed respectively；
(2) tea-seed peptide 1.5g, Peanut Polypeptide 1.7g, Grape Skin polypeptide 1.2g, sea-buckthorn polypeptide 2.1g, Chinese yam polypeptide are successively weighed
1.8g, yacon polypeptide 2.4g, oat polypeptide 3.8g, wild Fructus actinidiae chinensis polypeptide 3.0g, purple perilla polypeptide 2.5g, pure red date powder 48g,
Milk powder 32g is uniformly mixed；
(3) tabletting: the triangle chewable tablets finished product that side length is 2.1cm is obtained with single-punch tablet press tabletting.
A kind of preparation method of the fruits and vegetables natural polypeptides of embodiment 7 combination composition chewable tablet:
(1) by the natural Gly-His-Lys of fruits and vegetables, jujube, milk powder, 60 meshes be crushed respectively；
(2) fructus lycii polypeptide 2.8g, black fungus polypeptide 4.8g, golden Rosa roxburghii Tratt polypeptide 4.6g, taro polypeptide 2.8g are successively weighed, blueberry is more
Peptide 2.0g, pure red date powder 65g, milk powder 18g are uniformly mixed；
(3) the oval chewable tablets finished product of long 2.0cm wide 0.6cm tabletting: is obtained with single-punch tablet press tabletting.
A kind of preparation method of the fruits and vegetables natural polypeptides of embodiment 8 combination composition chewable tablet:
(1) by the natural Gly-His-Lys of fruits and vegetables, jujube, milk powder, 80 meshes be crushed respectively；
(2) dragon fruit polypeptide 1.5g, golden Rosa roxburghii Tratt polypeptide 2.5g, spirulina polypeptide 2.2g, peony seeds polypeptide 2.6g, ocean are successively weighed
Green onion polypeptide 1.8g, taro polypeptide 1.2g, oat polypeptide 2.2g, pure red date powder 58g, milk powder 28g are uniformly mixed；
(3) tabletting: diameter 2.5cm circle chewable tablets finished product is obtained with single-punch tablet press tabletting.
Fruits and vegetables natural polypeptides composition functional verification:
Test the test of 1. locomitivities
The fruits and vegetables natural polypeptides combination composition chewable tablet being prepared with any one of above-described embodiment 3-8 carries out improving body fortune
Kinetic force verifying: mouse is divided into control group (filling physiological saline), 1 group of experiment (it is natural to gavage the offer fruits and vegetables of embodiment 3 daily
Peptide composition 10mg/kg), 2 groups of experiment (gavage daily embodiment 5 provide fruits and vegetables natural polypeptides composition 20mg/kg).Respectively
Group animal 10 is raised 30 days.Groups of animals binds galvanized wire (calculating by the 5% of weight) in its tail portion respectively, carries out swimming with a load attached to the body
Swimming test, observation groups of animals were swum without the time being drowned, and result is as shown in figure 4, experimental group and control group exist
Significant difference (P < 0.001).As shown in Figure 4, compared with the control group, it takes fruits and vegetables natural polypeptides composition mouse and carries out swimming with a load attached to the body
The time of swimming extends, and dosage is bigger, and the time that swimming with a load attached to the body maintains is longer, it was demonstrated that fruits and vegetables natural polypeptides composition can be mentioned effectively
High body resisting fatigue ability.
The test of DPPH free radical is removed in test 2.
(1) it with the DPPH solution of dehydrated alcohol configuration 0.1mmol/L, is kept in dark place；
(2) fruits and vegetables natural polypeptides prepared by Example 3-8 either method combines composition chewable tablet, after grinding, is configured to concentration
The respectively solution of 0.5mg/mL, 1mg/mL, 2mg/mL, 5mg/mL places 5min, 3000rpm centrifugation after mixing well
5min takes supernatant stand-by；
(3) each 2 mL of above-mentioned various concentration solution is taken, the DPPH ethanol solution that 2 mL concentration are 0.1mmol/L is added,
It is uniformly mixed, reacts 30 min, 4000 rpm are centrifuged 10 min, supernatant is taken to measure light absorption value A at 517 nm1；
(4) above-mentioned 2 mL of each strength solution is taken respectively, and 2 mL dehydrated alcohols are added, light absorption value A is measured after reaction2；
(5) 2 mL concentration is taken to measure light absorption value A0 for the DPPH solution of 0.1mmol/L and the reaction of 2 mL dehydrated alcohols；
Clearance rate calculation formula are as follows: clearance rate (%)=[1-(A1-A2)/A0]×100
Each sample, which is repeated three times, to be averaged, and measurement result is shown in Table 1.
The ability of 1 fruits and vegetables natural polypeptides composition removing DPPH free radical of table
| Concentration (mg/ml) | 0.5 | 1 | 2 | 5 |
| Embodiment 4 | 35.2±2.37 | 68.57±2.49 | 83.13±2.65 | 90.87±1.56 |
| Embodiment 8 | 32.83±2.17 | 65.87±2.85 | 80.53±1.59 | 88.03±2.14 |
As can be seen from the results, with the increase of fruits and vegetables natural polypeptides composition solution concentration, remove the ability of DPPH free radical by
It is cumulative strong, illustrate that fruits and vegetables natural polypeptides composition has very strong Scavenging activity to DPPH free radical.
The above is only a preferred embodiment of the present invention, the present invention is not limited to above-mentioned preferred embodiment, any
People can obtain other various forms of products under the inspiration of the present invention, however, making any change in its shape or structure
Change, it is all that there is technical solution identical or similar to the present application, it is within the scope of the present invention.
Claims (8)
1. a kind of fruits and vegetables natural polypeptides combination composition chewable tablet and preparation method thereof, it is characterised in that the natural polypeptides composition nozzle
Piece is chewed to be made of the raw material of following mass fraction:
8-20 parts of fruits and vegetables natural polypeptides Gly-His-Lys,
Pure red date powder 45-80 parts,
12-32 parts of milk powder；
It carries out as steps described below:
(1) fruits and vegetables natural polypeptides Gly-His-Lys are prepared:
A. fresh fruit of vegetables tissue is cleaned, is diced, is homogenized under 2-8 DEG C of ice bath, precooled fruits and vegetables tissue weight 1-3 is then added
The phosphate buffer of 0.02-0.2 mol/L pH6.0-7.2 again impregnates, and after being placed in 2-8 DEG C of refrigerator 12 hours, 2-8 DEG C cold
Freeze centrifugation, supernatant is the crude extract containing fruits and vegetables natural polypeptides；
B. eluent is added into the crude extract containing fruits and vegetables natural polypeptides obtained by step a and is diluted most 30-100 containing polypeptide
The solution of mg/ml, is separated with sephadex chromatography, collects the component of molecular weight 500-12000 dalton；
C. the fruits and vegetables natural polypeptides collection liquid vacuum freeze drying in step b is lyophilized to get to fruits and vegetables natural polypeptides extract
Powder；
(2) it crushes: jujube is cut to drying and crushing after doing stoning；
(3) it is sieved: gained natural polypeptides freeze-dried powder, pure red date powder and milk powder in step (1) is crossed into -100 mesh of 60 mesh；
(4) mixing: natural polypeptides Gly-His-Lys, pure red date powder, the milk powder after sieving are uniformly mixed by above-mentioned mass fraction；
(5) uniformly mixed raw material tabletting: is obtained into chewable tablets finished product through tablet press machine direct tablet compressing.
2. fruits and vegetables natural polypeptides combination composition chewable tablet according to claim 1 and preparation method thereof, it is characterised in that described
Fruits and vegetables natural polypeptides combination composition chewable tablet is made of the raw material of following mass fraction:
12.5 parts of fruits and vegetables natural polypeptides Gly-His-Lys,
67.5 parts of pure red date powder,
20 parts of milk powder；
It carries out as steps described below:
(1) natural polypeptides Gly-His-Lys are prepared:
A. fresh fruit of vegetables tissue is cleaned, is diced, is homogenized under 2-8 DEG C of ice bath, precooled fruits and vegetables tissue weight 1-3 is then added
The phosphate buffer of 0.02-0.2 mol/L pH6.0-7.2 again impregnates, and after being placed in 2-8 DEG C of refrigerator 12 hours, 2-8 DEG C cold
Freeze centrifugation, supernatant is the crude extract containing fruits and vegetables natural polypeptides；
B. eluent is added into the crude extract containing fruits and vegetables natural polypeptides obtained by step a and is diluted most 30-100 containing polypeptide
The solution of mg/ml, is separated with sephadex chromatography, collects the component of molecular weight 500-12000 dalton；
C. the fruits and vegetables natural polypeptides collection liquid vacuum freeze drying in step b is lyophilized to get to fruits and vegetables natural polypeptides extract
Powder；
(2) it crushes: jujube is cut to drying and crushing after doing stoning；
(3) it is sieved: gained natural polypeptides freeze-dried powder, pure red date powder and milk powder in step (1) is crossed into -100 mesh of 60 mesh；
(4) mixing: natural polypeptides Gly-His-Lys, pure red date powder, the milk powder after sieving are uniformly mixed by above-mentioned mass fraction；
(5) uniformly mixed raw material tabletting: is obtained into chewable tablets finished product through tablet press machine direct tablet compressing.
3. fruits and vegetables natural polypeptides combination composition chewable tablet according to claim 1 to 2 and preparation method thereof, it is characterised in that institute
It is more selected from fructus lycii polypeptide, sweet potato leaves polypeptide, bitter gourd polypeptide, peony seeds polypeptide, Loquat-Shaped Cake polypeptide, yacon to state fruits and vegetables natural polypeptides
Peptide, oat polypeptide, onion polypeptide, purple perilla polypeptide, tea-seed peptide, semen sojae atricolor polypeptide, pumpkin flower polypeptide, walnut polypeptide, black fungus are more
Peptide, Peanut Polypeptide, dragon fruit polypeptide, golden Rosa roxburghii Tratt polypeptide, blueberry polypeptide, spirulina polypeptide, Grape Skin polypeptide, sea-buckthorn polypeptide, mountain
A variety of mixtures in the types such as medicine polypeptide, preserved egg powder polypeptide, wild Fructus actinidiae chinensis polypeptide, taro polypeptide, broccoli polypeptide.
4. preparation method according to claim 1 to 2, it is characterised in that: phosphate buffering liquid concentration described in step (1)
For 0.05 mol/L, the phosphate buffer pH is 7.0, and the buffer dosage is 2 times of fruits and vegetables tissue, the dilution
The solution containing polypeptide is 50 mg/mls afterwards.
5. preparation method according to claim 1 to 2, it is characterised in that: it is natural polypeptides freeze-dried powder described in step (3), pure
Red date powder and milk powder cross 80 meshes.
6. preparation method described in -6 according to claim 1, which is characterized in that the tabletting can be appointing convenient for oral taking chewy
Meaning shape.
7. natural polypeptides obtained by -7 any preparation methods combines composition chewable tablet according to claim 1, active constituent can
To be directly scattered in oral cavity and alimentary canal, can quickly absorb.
8. natural polypeptides combination composition chewable tablet obtained by -7 any preparation methods retains higher life according to claim 1
Reason activity, can be used for preparing the application in health food and food.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111248445A (en) * | 2020-03-11 | 2020-06-09 | 北京康立生医药技术开发有限公司 | Composition containing lycium barbarum polysaccharide for increasing bone mineral density |
| CN113826891A (en) * | 2021-09-02 | 2021-12-24 | 上海瑞贤高科生物科技有限公司 | Composition containing compound vitamin and tabletting preparation method and application thereof |
| CN115028697A (en) * | 2022-01-27 | 2022-09-09 | 昆明理工大学 | Hypoglycemic peptide and preparation method and application thereof |
-
2019
- 2019-01-29 CN CN201910086710.3A patent/CN109673751A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111248445A (en) * | 2020-03-11 | 2020-06-09 | 北京康立生医药技术开发有限公司 | Composition containing lycium barbarum polysaccharide for increasing bone mineral density |
| CN113826891A (en) * | 2021-09-02 | 2021-12-24 | 上海瑞贤高科生物科技有限公司 | Composition containing compound vitamin and tabletting preparation method and application thereof |
| CN115028697A (en) * | 2022-01-27 | 2022-09-09 | 昆明理工大学 | Hypoglycemic peptide and preparation method and application thereof |
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Application publication date: 20190426 |