CN109673723A - Fish natural biological freshness-preserving agent and its preparation and application - Google Patents
Fish natural biological freshness-preserving agent and its preparation and application Download PDFInfo
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- CN109673723A CN109673723A CN201910077836.4A CN201910077836A CN109673723A CN 109673723 A CN109673723 A CN 109673723A CN 201910077836 A CN201910077836 A CN 201910077836A CN 109673723 A CN109673723 A CN 109673723A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/10—Coating with a protective layer; Compositions or apparatus therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to technical field of aquatic product preservation, a kind of fish natural biological freshness-preserving agent and its preparation and application are disclosed, is comprised the following components in parts by weight: 14-28 parts of rosemary extracting solution, 6-16 parts of root of Chinese clematis extracting solution, 2-10 parts of Lygodium japonicum extracting solution, 10-20 parts of angelica anomala extracting solution, 4-10 parts of rhizoma polygonati extracting solution, 2-8 parts of thizoma curculiginis extracting solution, 6-13 parts of wild chrysanthemum extracting solution, 3-7 parts of tea polyphenols, 2-5 parts of D-sorbite, 2-5 parts of pH adjusting agent, 4-8 parts of cellulase.Compared with prior art, antistaling agent in the present invention can effectively avoid that freeze preservation bring fish musculature structure is destroyed, juice loss is serious and nutrition loss etc., avoid the remaining food-safety problem of chemical preservative bring preservative, the circulation sale for guaranteeing fresh fish is a kind of green, environmental protection, noresidue, the significant antistaling agent of low in cost, safe and reliable and fresh-keeping effect.
Description
Technical field
The present invention relates to technical field of aquatic product preservation, in particular to a kind of fish natural biological freshness-preserving agent and its preparation and
Application method.
Background technique
Fish are full of nutrition because of its delicious flavour, have the characteristics that low fat, high protein and highly unsaturated fatty acid,
It is popular among consumers, early have become the important sources that people absorb animal protein.In China, the stock of fish is abundant, kind
Class is various, and the fishing phase Relatively centralized of fish, and moisture and protein content are high, carries a large amount of microorganisms, if after fish are dead
Appropriate Storage Measures are taken not in time, easily occur putrid and deteriorated, influence the processing, transport and sale of fish.Therefore, to catching
Fish after fishing should take appropriate fresh-keeping measure to prevent its putrid and deteriorated in time.
Currently, the preservation and freshness mode that fish generally use is freeze preservation and ice temperature fresh-keeping.Refrigeration fresh-keeping technique is suitable
A kind of method for fish long-term storage.But will form ice crystal during freezing due to the flesh of fish leads to albuminous degeneration, ice crystal
It gradually grows up and also results in muscular tissue structure and completely destroy, serious juice loss occurs when product being caused to thaw, and thaw
Speed is slow, and the discoloration of meat surface oxidation influences mouthfeel, nutrition loss is serious.Ice temperature fresh-keeping, which refers to, is placed in 0 DEG C hereinafter, freezing for fish
Within the scope of temperature more than node, it is made to keep low temperature and uncongealable state, temperature range is generally 0~-2 DEG C, and ice temperature is protected
It is fresh to play the role of low temperature stress to biological cell, the condition of living organism of cell is kept, fresh fish product can be preferably kept
Matter.But the negligible amounts of the standardized ice Wen Ku in China at this stage, reserve temperature control accuracy requirement of the ice temperature fresh-keeping to freezer
Higher, the temperature fluctuation in storage cannot be too big, needs that cold air is selected to be evenly distributed, the good freezer of thermal storage performance,
Ask the temperature control precision of ice-temperature freshness-preservation storehouse at ± 0.5~1.0 DEG C, and the precision of temperature of ice house control at present is generally at ± 1.0 DEG C
More than, and costly, this restrict the applications of China's ice-temperature technique for Icetemperature Storage equipment compared with commercial refrigerator.Traditional change
Although learning, preservative is fresh-keeping can effectively to extend fish storage time and quality, and fish body can remain preservative, give human body band
Carry out a degree of potential hazard, safety is low, reduces its edible value.
Therefore, it avoids that freeze preservation bring muscular tissue structure is destroyed, juice loss is serious and nutrition loss etc. is unfavorable,
It avoids in ice temperature fresh-keeping temperature from the disadvantages such as being difficult to, the remaining food safety of chemical preservative bring preservative is avoided to ask
Topic realizes the circulation sale of really fresh fish, it is necessary to study a kind of green, environmental protection, noresidue, it is low in cost, safely can
It leans on and a kind of significant natural plant antistaling agent of fresh-keeping effect.
Summary of the invention
Goal of the invention: aiming at the problems existing in the prior art, the present invention provide a kind of fish natural biological freshness-preserving agent and
Its preparation and application, the antistaling agent can effectively avoid the destruction of freeze preservation bring fish musculature structure, juice
It is lost serious and nutrition loss etc., the remaining food-safety problem of chemical preservative bring preservative is avoided, guarantees fresh fish
The circulation sale of class is a kind of green, environmental protection, noresidue, the significant antistaling agent of low in cost, safe and reliable and fresh-keeping effect.
Technical solution: the present invention provides a kind of fish natural biological freshness-preserving agents, comprise the following components in parts by weight: fan changes
Fragrant extracting solution 14-28 parts, 6-16 parts of root of Chinese clematis extracting solution, 2-10 parts of Lygodium japonicum extracting solution, 10-20 parts of angelica anomala extracting solution, Huang
Smart extracting solution 4-10 parts, 2-8 parts of thizoma curculiginis extracting solution, 6-13 parts of wild chrysanthemum extracting solution, 3-7 parts of tea polyphenols, 2-5 parts of D-sorbite,
2-5 parts of pH adjusting agent, 4-8 parts of cellulase.
Preferably, the fish natural biological freshness-preserving agent is grouped as by the group of following parts by weight: rosemary extracting solution 14
Part, 6 parts of root of Chinese clematis extracting solution, 2 parts of Lygodium japonicum extracting solution, 10 parts of angelica anomala extracting solution, 4 parts of rhizoma polygonati extracting solution, thizoma curculiginis extracting solution 2
Part, 6 parts of wild chrysanthemum extracting solution, 3 parts of tea polyphenols, 2 parts of D-sorbite, 2 parts of pH adjusting agent, 4 parts of cellulase.
Preferably, the fish natural biological freshness-preserving agent is grouped as by the group of following parts by weight: rosemary extracting solution 21
Part, 11 parts of root of Chinese clematis extracting solution, 6 parts of Lygodium japonicum extracting solution, 15 parts of angelica anomala extracting solution, 7 parts of rhizoma polygonati extracting solution, thizoma curculiginis extracting solution
5 parts, 9 parts of wild chrysanthemum extracting solution, 5 parts of tea polyphenols, 3 parts of D-sorbite, 3 parts of pH adjusting agent, 6 parts of cellulase.
Preferably, the fish natural biological freshness-preserving agent is grouped as by the group of following parts by weight: rosemary extracting solution 28
Part, 16 parts of root of Chinese clematis extracting solution, 10 parts of Lygodium japonicum extracting solution, 20 parts of angelica anomala extracting solution, 10 parts of rhizoma polygonati extracting solution, thizoma curculiginis are extracted
8 parts of liquid, 13 parts of wild chrysanthemum extracting solution, 7 parts of tea polyphenols, 5 parts of D-sorbite, 5 parts of pH adjusting agent, 8 parts of cellulase.
The present invention also provides a kind of preparation methods of fish natural biological freshness-preserving agent, by rosemary extracting solution, the root of Chinese clematis
Extracting solution, Lygodium japonicum extracting solution, angelica anomala extracting solution, rhizoma polygonati extracting solution, thizoma curculiginis extracting solution and wild chrysanthemum extracting solution are by weight
Allotment, adds tea polyphenols, D-sorbite and pH adjusting agent, stirs evenly in beaker, obtain mixed liquor, be put into high steam
Autoclave, sterilize 15min under the conditions of 121 DEG C, cooling, obtains the fish natural biological freshness-preserving agent.
The present invention also provides a kind of application methods of fish natural biological freshness-preserving agent, and the fish natural biological is fresh-keeping
Agent adds distilled water to dilute 2-3 times, by the fish day of the wash clean after fresh-keeping fish are soaked in dilution at 4-8 DEG C of temperature
In right bio-preservative, 20-30min is impregnated, then fish are taken out, be sealed.
The utility model has the advantages that the fish natural biological freshness-preserving agent in the present invention is by Rosmarinus officinalis extract, the root of Chinese clematis, Lygodium japonicum, river
The root of Dahurain angelica, rhizoma polygonati, thizoma curculiginis, wild chrysanthemum, tea polyphenols, D-sorbite and pH adjusting agent composition；Wherein,
Rosmarinus officinalis extract is the efficient natural antioxidant extracted from rosemary, mainly includes that terpene, flavones, phenols etc. are anti-
The growth of psychrophile and saccharomycete can be effectively suppressed in bacterium active material and antioxidant, can be by slowing down the chain of free radical
Formula reaction, effectively slows down protein degradation and fat oxidation.
The root of Chinese clematis is the root and rhizome of the Ranunculaceae climber shrub root of Chinese clematis, and selection item is long, skin is black, meat is white, the solid person of matter
It is preferred, the root of Chinese clematis is distributed mainly on Chinese yunnan south, Guizhou, Sichuan, Guangxi, Guangdong, Zhejiang, South of Jiangsu Province, Anhui Huaihe River
On the south etc. ground.Type specimen picks up from south of Yunnan.Have effects that analgesia, wind-damp dispelling, degrading the channel, to gram-positive bacteria, leather
Lan Shi negative bacterium and mould have stronger inhibiting effect.
Lygodium japonicum is the mature spore of Lygodiaceae pteridophyte Lygodium japonicum, and main product is in Guangdong, Zhejiang, Jiangsu, Hunan etc.
Ground, selection body is dry, grain is thin, can float on water, the quick-fried loud person of combustion is preferred.Lygodium japonicum has staphylococcus aureus, Pseudomonas aeruginosa aobvious
Write inhibiting effect.
Angelica anomala is the root of umbelliferae angelica anomala, and main product is in Sichuan, and selection root item is coarse, skin is thin, mealiness is sufficient, fragrance
Dense person is preferred.Angelica anomala decoction has inhibiting effect to Escherichia coli, typhoid bacillus, dermatophyte etc..
Rhizoma polygonati is the rhizome of liliaceous plant rhizoma polygonati, P. cyrtonema, hot river rhizoma polygonati etc., and main product is in northeast and Hebei, Shandong
Etc. ground.Selection block is big, color is yellow, section is transparent, matter is moistened, and person is preferred.The main component of rhizoma polygonati has Siberian solomonseal rhizome polysaccharide, oligosaccharide, relies ammonia
The equal 11 kinds of amino acid of acid and palm leaf coculine, jateorrhizine, paAlmatine, fibrauretine, fibralactone, sterol, niacin and zinc,
The microelements such as copper, iron.There is inhibiting effect to a variety of harmful bacterias such as staphylococcus aureus, typhoid bacillus, acid fast bacteria.
Thizoma curculiginis is that the drying of amrallid thizoma curculiginis or fresh rhizome, main product are chosen in Sichuan, Yunnan, Guizhou and other places
Body is dry, sturdy, the black person of the hard color of matter is preferred.This product is containing lycorine, yuccagenin, curculigoside A and curculigoside B, orcin, grape
The ingredients such as glucosides and nitrogen compound, sterol, fat compound.With calmness, extend sleeping time, it is anticonvulsion, anti-
The effects of bacterium, anti-inflammatory, anticancer, antimutation.
Wild chrysanthemum is composite family herbaceos perennial, wild in the wildly band such as patana, Tian Bian, roadside.With color Huang
It is preferred without stalk, complete, toil, the non-standard-sized sheet person of flower.Wild chrysanthemum cold nature, tool dispelling wind and heat from the body, subdhing swelling and detoxicating and other effects.To golden yellow
Staphylococcus, corynebacterium diphtheriae, streptococcus, pseudomonas aeruginosa, shigella dysenteriae, influenza virus have inhibiting effect.
Compared with prior art, the invention has the following advantages that
(1) present invention has effects that fresh-keeping, anti-corrosion, can effectively inhibit the growth of microorganism, slows down protein degradation and fat
Oxidation, the speed for delaying food to rot, the fresh-keeping fish of antistaling agent through the invention can be with Shelf-life.
Fish natural biological freshness-preserving agent in the present invention by Rosmarinus officinalis extract, the root of Chinese clematis, Lygodium japonicum, angelica anomala, rhizoma polygonati,
Thizoma curculiginis and wild chrysanthemum composition, contained active constituent is different, also not identical to coverage, the function and effect of microorganism, has
It is broad spectrum antibiotic, has plenty of antimycotic, has plenty of antiviral, they are combined, the range of inhibited microorganism becomes
Extensively, multiple-microorganism growth and breeding can be inhibited more significantly or kill bacterium, TVB-N value is effectively controlled, slow down significantly
Protein degradation, to keep fish meat freshness.The fresh-keeping effect same with freeze preservation can be reached under cryogenic.It keeps away
Hydrone freezes to generate ice crystal when having exempted from freeze preservation and gradually growing up for ice crystal will lead to muscular tissue structure completely by broken
Bad situation protects the integrality of structure of fish muscle, maintains preferable mouthfeel.In addition Rosmarinus officinalis extract and Spora Lygodii extract
Also there is strong antioxidation, greatly reduce the oxidation rate of unsaturated fatty acid, shorten the time of Preservation Treatment, but goods
The frame phase is but extended, save the cost and time.
(2) after this fish natural biological freshness-preserving agent is smeared on fish body surface, in the adhesive effect of the ingredients such as Lygodium japonicum, thizoma curculiginis
Under, it can produce biological protection film, prevent external microorganism from polluting to fish, while being able to maintain fish moisture content,
Reduce flesh of fish juice loss.
(4) raw material that the present invention uses derives from natural plants and its extract, is a kind of natural biological freshness-preserving agent, peace
It is complete effectively only to need clear water cleaning that remove the antistaling agent on fish after use, it is safe and harmless to people, and the mouth of fish is not influenced
Sense.
(5) raw material sources of the present invention are extensive, are easily obtained and low in cost, have a wide range of application, can be used for fresh fish, shrimp,
Circulation, storage and the processing of the fish such as shellfish.
Specific embodiment
The present invention is described in detail With reference to embodiment.
Embodiment 1:
Present embodiments provide for a kind of fish natural biological freshness-preserving agents, are grouped as by the group of following parts by weight: rosemary extracts
14 parts of liquid, 6 parts of root of Chinese clematis extracting solution, 2 parts of Lygodium japonicum extracting solution, 10 parts of angelica anomala extracting solution, 4 parts of rhizoma polygonati extracting solution, thizoma curculiginis mention
Take 2 parts of liquid, 6 parts of wild chrysanthemum extracting solution, 3 parts of tea polyphenols, 2 parts of D-sorbite, 2 parts of pH adjusting agent, 4 parts of cellulase.
The preparation process of above-mentioned fish natural biological freshness-preserving agent is as follows:
(1) preparation of root of Chinese clematis extracting solution, the specific method is as follows:
Take root of Chinese clematis rhizome 1000g, clean 3min with tap water, remove surface impurity, at room temperature draining 30min to surface without
Moisture is placed in the jaw crusher that sieve bore dia is 4mm and is crushed, and obtains the particle that partial size is less than 4mm, and concentration is added and is
The cellulase aqueous solution of 60-70% is impregnated 3h with small grafter after mixing evenly, is then put into dipped particle surface 1-2cm
In DHG-9040A air dry oven, dry 6h, taking-up are placed on cooled to room temperature in pallet under the conditions of temperature is 50 DEG C,
It is put into YC-06B pulverizer and 5min is crushed with the revolving speed of 300rmp, cross 400 meshes, obtain Radix Clematidis extract.The root of Chinese clematis mentions
It takes object to mix with 80% ethyl alcohol by solid-liquid ratio 1:8, impregnates 2h, leachate is extracted 2 times using reflux extraction, 2 is small every time
When, merging filtrate is removed the gred with 1 layer of filtered through gauze, obtains root of Chinese clematis extracting solution, spare.
(2) preparation of Lygodium japonicum extracting solution, the specific method is as follows:
50 DEG C of drying, are put into the revolving speed powder in YC-06B pulverizer with 300rmp after taking fresh Lygodium japonicum 1000g, room temperature to dry in the shade
Broken 5min, 100 mesh sieving, obtains Lygodium japonicum powder, and Lygodium japonicum powder is mixed with 80% ethyl alcohol by solid-liquid ratio 1:20, and 45-55 DEG C
2 h of refluxing extraction is filtered to remove residue with 1 layer of gauze while hot, and merging filtrate obtains Lygodium japonicum extracting solution.
(3) preparation of angelica anomala extracting solution, the specific method is as follows:
1200g angelica anomala is weighed, is put into YC-06B pulverizer and 10min is crushed with the revolving speed of 400rmp, 400 meshes is crossed, obtains
Angelica anomala powder；Angelica anomala powder is mixed with 75% ethyl alcohol by solid-liquid ratio 1:8, immersion 2 hours, and heating and refluxing extraction 2 times, often
Secondary 2 hours, merging filtrate was removed the gred with 1 layer of filtered through gauze, and filtrate passes through 0.22 μm of sterilised membrane filter filtration sterilization, and cryo-conservation is standby
With.
(4) preparation of rhizoma polygonati concentrate, the specific method is as follows:
The rhizoma polygonati rhizome 800g that block is big, color is yellow, section is transparent is chosen, slice is put into YC-06B pulverizer, is turned through 400rmp
Speed crushes 5min, crosses 80 meshes, obtains Solomonseal Rhizome；Water is added according to the ratio of solid-liquid ratio 1:18 in Solomonseal Rhizome and impregnates 1.5h,
And be put under the ultrasonic instrument that power is 150W and act on 55min, temperature is 70 DEG C, obtains solidliquid mixture；By solidliquid mixture
It is added in reactor tank, temperature is adjusted to 45 DEG C in reactor tank, and pH value 6.0 puts into protease, and solid-liquid ratio 1:1.5 is lasting to stir
1-2h carries out proteolysis, obtains enzymolysis liquid；Enzymolysis liquid is filtered, 10 DEG C of low-temperature reduced-pressures are concentrated into the 1/3 of original volume, it is dense to obtain rhizoma polygonati
Contracting liquid.
(5) preparation of thizoma curculiginis extracting solution, the specific method is as follows:
The thizoma curculiginis rhizome for weighing 400g is put into YC-06B pulverizer and crushes 10min with the revolving speed of 400rmp, crosses 400 meshes, obtains
To Common Curculigo Rhizome；Common Curculigo Rhizome and distilled water solid-liquid ratio are 1:5, decoct and extract 1 time, 1 hour every time, filtering obtained thizoma curculiginis and mentions
Take liquid.
(6) preparation of wild chrysanthemum extracting solution, the specific method is as follows:
The wild chrysanthemum for weighing 800g is put into YC-06B pulverizer and crushes 10-15min with the revolving speed of 400rmp, crosses 400 meshes,
Obtain wild chrysanthemum powder；Wild chrysanthemum powder and distilled water solid-liquid ratio are 1:5, decoct and extract 1 time, 1 hour every time, filtering obtained
Wild chrysanthemum extracting solution.
(7) preparation of rosemary extracting solution, the specific method is as follows:
200g Rosmarinus officinalis extract is weighed, Rosmarinus officinalis extract is sufficiently dissolved, and stir evenly with distilled water, is configured to 0.1%
The solution of concentration is put in spare in beaker.
(8) preparation of fish natural biological freshness-preserving agent:
Root of Chinese clematis extracting solution in step (1)-(7), Lygodium japonicum extracting solution, angelica anomala extracting solution, rhizoma polygonati concentrate, thizoma curculiginis are mentioned
It takes liquid, wild chrysanthemum extracting solution and rosemary extracting solution to deploy by weight, adds tea polyphenols, D-sorbite and pH adjusting agent,
It is stirred evenly in beaker, obtains mixed liquor, be put into high-pressure steam sterilizing pan, sterilize 15min under the conditions of 121 DEG C, and it is cooling, it obtains
To fish natural biological freshness-preserving agent.
(9) it by fish natural biological freshness-preserving agent container package, is stored at drying.
Application method: add distilled water to dilute 1 times fish natural biological freshness-preserving agent, to be processed, wash clean fish are existed
In fish natural biological freshness-preserving agent after being soaked in dilution at 4 DEG C of temperature, 20min is impregnated, then fish are taken out, be sealed.
Embodiment 2:
Present embodiments provide for a kind of fish natural biological freshness-preserving agents, are grouped as by the group of following parts by weight: rosemary extracts
21 parts of liquid, 11 parts of root of Chinese clematis extracting solution, 6 parts of Lygodium japonicum extracting solution, 15 parts of angelica anomala extracting solution, 7 parts of rhizoma polygonati extracting solution, thizoma curculiginis mention
Take 5 parts of liquid, 9 parts of wild chrysanthemum extracting solution, 5 parts of tea polyphenols, 3 parts of D-sorbite, 3 parts of pH adjusting agent, 6 parts of cellulase.
The preparation process of above-mentioned fish natural biological freshness-preserving agent is as follows:
(1) preparation of root of Chinese clematis extracting solution, the specific method is as follows:
Take root of Chinese clematis rhizome 1300g, clean 4min with tap water, remove surface impurity, at room temperature draining 40min to surface without
Moisture is placed in the jaw crusher that sieve bore dia is 4mm and is crushed, and obtains the particle that partial size is less than 4mm, and concentration is added and is
The cellulase aqueous solution of 60-70% is impregnated 3.5h with small grafter after mixing evenly, is then put into dipped particle surface 1.5cm
In DHG-9040A air dry oven, dry 7h, taking-up are placed on cooled to room temperature in pallet under the conditions of temperature is 55 DEG C,
It is put into YC-06B pulverizer and 8min is crushed with the revolving speed of 400rmp, cross 400 meshes, obtain Radix Clematidis extract.The root of Chinese clematis mentions
Take object to mix with 80% ethyl alcohol by solid-liquid ratio 1:8, impregnate 3h, by leachate using reflux extraction extract 3 times, 3 hours every time,
Merging filtrate is removed the gred with 2 layers of filtered through gauze, obtains root of Chinese clematis extracting solution, spare.
(2) preparation of Lygodium japonicum extracting solution, the specific method is as follows:
55 DEG C of drying, are put into the revolving speed powder in YC-06B pulverizer with 400rmp after taking fresh Lygodium japonicum 1300g, room temperature to dry in the shade
Broken 8min, 100 mesh sieving, obtains Lygodium japonicum powder, and Lygodium japonicum powder is mixed with 80% ethyl alcohol by solid-liquid ratio 1:20, and 50 DEG C are returned
Stream extracts 3 h, is filtered to remove residue while hot with 2 layers of gauze, merging filtrate obtains Lygodium japonicum extracting solution.
(3) preparation of angelica anomala extracting solution, the specific method is as follows:
1500g angelica anomala is weighed, is put into YC-06B pulverizer and 12min is crushed with the revolving speed of 400rmp, 400 meshes is crossed, obtains
Angelica anomala powder；Angelica anomala powder is mixed with 75% ethyl alcohol by solid-liquid ratio 1:8, immersion 3 hours, and heating and refluxing extraction 3 times, every time
3 hours, merging filtrate was removed the gred with 2 layers of filtered through gauze, and filtrate passes through 0.22 μm of sterilised membrane filter filtration sterilization, and cryo-conservation is standby
With.
(4) preparation of rhizoma polygonati concentrate, the specific method is as follows:
The rhizoma polygonati rhizome 900g that block is big, color is yellow, section is transparent is chosen, slice is put into YC-06B pulverizer, is turned through 400rmp
Speed crushes 7min, crosses 80 meshes, obtains Solomonseal Rhizome；Water is added according to the ratio of solid-liquid ratio 1:18 in Solomonseal Rhizome and impregnates 1.5h,
And be put under the ultrasonic instrument that power is 150W and act on 62min, temperature is 75 DEG C, obtains solidliquid mixture；By solidliquid mixture
It is added in reactor tank, temperature is adjusted to 52 DEG C in reactor tank, and pH value 7.0 puts into protease, and solid-liquid ratio 1:1.5 is lasting to stir
1-2h carries out proteolysis, obtains enzymolysis liquid；Enzymolysis liquid is filtered, low-temperature reduced-pressure is concentrated into the 1/3 of original volume, and 20 DEG C of low-temperature reduced-pressures are dense
It is reduced to the 1/3 of original volume, obtains rhizoma polygonati concentrate.
(5) preparation of thizoma curculiginis extracting solution, the specific method is as follows:
The thizoma curculiginis rhizome for weighing 500g is put into YC-06B pulverizer and crushes 12min with the revolving speed of 400rmp, crosses 400 meshes, obtains
To Common Curculigo Rhizome；Common Curculigo Rhizome and distilled water solid-liquid ratio are 1:6, decoct and extract 2 times, 2 hours every time, filtering obtained thizoma curculiginis and mentions
Take liquid.
(6) preparation of wild chrysanthemum extracting solution, the specific method is as follows:
The wild chrysanthemum for weighing 900g is put into YC-06B pulverizer and crushes 12min with the revolving speed of 400rmp, crosses 400 meshes, obtains
Wild chrysanthemum powder；Wild chrysanthemum powder and distilled water solid-liquid ratio are 1:6, decoct and extract 2 times, 2 hours every time, filtering obtained mother chrysanthemum
Flower extracting solution.
(7) preparation of rosemary extracting solution, the specific method is as follows:
400g Rosmarinus officinalis extract is weighed, Rosmarinus officinalis extract is sufficiently dissolved, and stir evenly with distilled water, is configured to 0.5%
The solution of concentration is put in spare in beaker.
(8) preparation of fish natural biological freshness-preserving agent:
Root of Chinese clematis extracting solution in step (1)-(7), Lygodium japonicum extracting solution, angelica anomala extracting solution, rhizoma polygonati concentrate, thizoma curculiginis are mentioned
It takes liquid, wild chrysanthemum extracting solution and rosemary extracting solution to deploy by weight, adds tea polyphenols, D-sorbite and pH adjusting agent,
It is stirred evenly in beaker, obtains mixed liquor, be put into high-pressure steam sterilizing pan, sterilize 15min under the conditions of 121 DEG C, and it is cooling, it obtains
To fish natural biological freshness-preserving agent.
(9) it by fish natural biological freshness-preserving agent container package, is stored at drying.
Application method: add distilled water to dilute 2 times fish natural biological freshness-preserving agent, to be processed, wash clean fish are existed
In fish natural biological freshness-preserving agent after being soaked in dilution at 6 DEG C of temperature, 25min is impregnated, then fish are taken out, be sealed.
Embodiment 3:
Present embodiments provide for a kind of fish natural biological freshness-preserving agents, are grouped as by the group of following parts by weight: rosemary extracts
28 parts of liquid, 16 parts of root of Chinese clematis extracting solution, 10 parts of Lygodium japonicum extracting solution, 20 parts of angelica anomala extracting solution, 10 parts of rhizoma polygonati extracting solution, thizoma curculiginis
8 parts of extracting solution, 13 parts of wild chrysanthemum extracting solution, 7 parts of tea polyphenols, 5 parts of D-sorbite, 5 parts of pH adjusting agent, 8 parts of cellulase.
The preparation process of above-mentioned fish natural biological freshness-preserving agent is as follows:
(1) preparation of root of Chinese clematis extracting solution, the specific method is as follows:
Take root of Chinese clematis rhizome 1500g, clean 5min with tap water, remove surface impurity, at room temperature draining 45min to surface without
Moisture is placed in the jaw crusher that sieve bore dia is 5mm and is crushed, and obtains the particle that partial size is less than 5mm, and concentration is added and is
The cellulase aqueous solution of 60-70% impregnates 4h with small grafter after mixing evenly, is then put into DHG- to dipped medicinal material surface 2cm
In 9040A air dry oven, dry 8h, taking-up are placed on cooled to room temperature in pallet, are put under the conditions of temperature is 60 DEG C
10min is crushed with the revolving speed of 500rmp in YC-06B pulverizer, 500 meshes is crossed, obtains Radix Clematidis extract.Radix Clematidis extract
It is mixed with 80% ethyl alcohol by solid-liquid ratio 1:8, impregnates 4h, leachate is extracted 4 times using reflux extraction, 4 hours every time, is closed
And filtrate, it is removed the gred with 3 layers of filtered through gauze, obtains root of Chinese clematis extracting solution, it is spare.
(2) preparation of Lygodium japonicum extracting solution, the specific method is as follows:
60 DEG C of drying, are put into the revolving speed powder in YC-06B pulverizer with 500rmp after taking fresh Lygodium japonicum 1500g, room temperature to dry in the shade
Broken 10min, 110 mesh sieving, obtains Lygodium japonicum powder, and Lygodium japonicum powder is mixed with 80% ethyl alcohol by solid-liquid ratio 1:20, and 55 DEG C
4 h of refluxing extraction is filtered to remove residue with 3 layers of gauze while hot, and merging filtrate obtains Lygodium japonicum extracting solution.
(3) preparation of angelica anomala extracting solution, the specific method is as follows:
1700g angelica anomala is weighed, is put into YC-06B pulverizer and 15min is crushed with the revolving speed of 500rmp, 500 meshes is crossed, obtains
Angelica anomala powder；Angelica anomala powder is mixed with 75% ethyl alcohol by solid-liquid ratio 1:8, immersion 4 hours, and heating and refluxing extraction 4 times, often
Secondary 4 hours, merging filtrate was removed the gred with 3 layers of filtered through gauze, and filtrate passes through 0.22 μm of sterilised membrane filter filtration sterilization, and cryo-conservation is standby
With.
(4) preparation of rhizoma polygonati concentrate, the specific method is as follows:
The rhizoma polygonati rhizome 1000g that block is big, color is yellow, section is transparent is chosen, slice is put into YC-06B pulverizer, through 500rmp's
Revolving speed crushes 10min, crosses 80 meshes, obtains Solomonseal Rhizome；Water is added according to the ratio of solid-liquid ratio 1:18 in Solomonseal Rhizome to impregnate
1.5h, and be put under the ultrasonic instrument that power is 160W and act on 70min, temperature is 80 DEG C, obtains solidliquid mixture；Solid-liquid is mixed
It closes object to be added in reactor tank, temperature is adjusted to 60 DEG C in reactor tank, and pH value 8.0 puts into protease, and solid-liquid ratio 1:1.5 continues
It stirs 2h and carries out proteolysis, obtain enzymolysis liquid；Enzymolysis liquid is filtered, 30 DEG C of low-temperature reduced-pressures are concentrated into the 1/3 of original volume, obtain rhizoma polygonati
Concentrate.
(5) preparation of thizoma curculiginis extracting solution, the specific method is as follows:
The thizoma curculiginis rhizome for weighing 600g is put into YC-06B pulverizer and crushes 15min with the revolving speed of 500rmp, crosses 500 meshes, obtains
To Common Curculigo Rhizome；Common Curculigo Rhizome and distilled water solid-liquid ratio are 1:8, decoct and extract 3 times, 3 hours every time, filtering obtained thizoma curculiginis and mentions
Take liquid.
(6) preparation of wild chrysanthemum extracting solution, the specific method is as follows:
The wild chrysanthemum for weighing 1000g is put into YC-06B pulverizer and crushes 15min with the revolving speed of 500rmp, crosses 500 meshes, obtains
To wild chrysanthemum powder；Wild chrysanthemum powder and distilled water solid-liquid ratio are 1:8, decoct and extract 3 times, 3 hours every time, filtering obtained open country
Chrysanthemum extract liquid.
(7) preparation of rosemary extracting solution, the specific method is as follows:
600g Rosmarinus officinalis extract is weighed, Rosmarinus officinalis extract is sufficiently dissolved, and stir evenly with distilled water, is configured to 1.0%
The solution of concentration is put in spare in beaker.
(8) preparation of fish natural biological freshness-preserving agent:
Root of Chinese clematis extracting solution in step (1)-(7), Lygodium japonicum extracting solution, angelica anomala extracting solution, rhizoma polygonati concentrate, thizoma curculiginis are mentioned
It takes liquid, wild chrysanthemum extracting solution and rosemary extracting solution to deploy by weight, adds tea polyphenols, D-sorbite and pH adjusting agent,
It is stirred evenly in beaker, obtains mixed liquor, be put into high-pressure steam sterilizing pan, sterilize 15min under the conditions of 121 DEG C, and it is cooling, it obtains
To fish natural biological freshness-preserving agent.
(9) it by fish natural biological freshness-preserving agent container package, is stored at drying.
Application method: add distilled water to dilute 3 times fish natural biological freshness-preserving agent, to be processed, wash clean fish are existed
In fish natural biological freshness-preserving agent after being soaked in dilution at 8 DEG C of temperature, 30min is impregnated, then fish are taken out, be sealed.
The technical concepts and features of above embodiment only to illustrate the invention, its object is to allow be familiar with technique
People cans understand the content of the present invention and implement it accordingly, and it is not intended to limit the scope of the present invention.It is all according to the present invention
The equivalent transformation or modification that Spirit Essence is done, should be covered by the protection scope of the present invention.
Claims (6)
1. a kind of fish natural biological freshness-preserving agent, which is characterized in that comprise the following components in parts by weight: rosemary extracting solution 14-
28 parts, 6-16 parts of root of Chinese clematis extracting solution, 2-10 parts of Lygodium japonicum extracting solution, 10-20 parts of angelica anomala extracting solution, rhizoma polygonati extracting solution 4-10
Part, 2-8 parts of thizoma curculiginis extracting solution, 6-13 parts of wild chrysanthemum extracting solution, 3-7 parts of tea polyphenols, 2-5 parts of D-sorbite, pH adjusting agent 2-5
Part, 4-8 parts of cellulase.
2. fish natural biological freshness-preserving agent according to claim 1, which is characterized in that be grouped by the group of following parts by weight
At: 14 parts of rosemary extracting solution, 6 parts of root of Chinese clematis extracting solution, 2 parts of Lygodium japonicum extracting solution, 10 parts of angelica anomala extracting solution, rhizoma polygonati extract
4 parts of liquid, 2 parts of thizoma curculiginis extracting solution, 6 parts of wild chrysanthemum extracting solution, 3 parts of tea polyphenols, 2 parts of D-sorbite, 2 parts of pH adjusting agent, cellulose
4 parts of enzyme.
3. fish natural biological freshness-preserving agent according to claim 1, which is characterized in that be grouped by the group of following parts by weight
At: 21 parts of rosemary extracting solution, 11 parts of root of Chinese clematis extracting solution, 6 parts of Lygodium japonicum extracting solution, 15 parts of angelica anomala extracting solution, rhizoma polygonati mention
Take 7 parts of liquid, 5 parts of thizoma curculiginis extracting solution, 9 parts of wild chrysanthemum extracting solution, 5 parts of tea polyphenols, 3 parts of D-sorbite, 3 parts of pH adjusting agent, fiber
6 parts of plain enzyme.
4. fish natural biological freshness-preserving agent according to claim 1, which is characterized in that be grouped by the group of following parts by weight
At: 28 parts of rosemary extracting solution, 16 parts of root of Chinese clematis extracting solution, 10 parts of Lygodium japonicum extracting solution, 20 parts of angelica anomala extracting solution, rhizoma polygonati mention
Take 10 parts of liquid, 8 parts of thizoma curculiginis extracting solution, 13 parts of wild chrysanthemum extracting solution, 7 parts of tea polyphenols, 5 parts of D-sorbite, 5 parts of pH adjusting agent, fibre
Tie up plain 8 parts of enzyme.
5. a kind of preparation method of fish natural biological freshness-preserving agent according to any one of claims 1 to 4, feature exist
In by rosemary extracting solution, root of Chinese clematis extracting solution, Lygodium japonicum extracting solution, angelica anomala extracting solution, rhizoma polygonati extracting solution, thizoma curculiginis extraction
Liquid and wild chrysanthemum extracting solution are deployed by weight, add tea polyphenols, D-sorbite and pH adjusting agent, are stirred in beaker equal
It is even, mixed liquor is obtained, high-pressure steam sterilizing pan is put into, sterilize 15min under the conditions of 121 DEG C, and it is cooling, it is natural to obtain the fish
Bio-preservative.
6. a kind of application method of fish natural biological freshness-preserving agent according to any one of claims 1 to 4, feature exist
In, by the fish natural biological freshness-preserving agent add distilled water dilute 2-3 times, wait for fresh-keeping fish at 4-8 DEG C of temperature wash clean
Under be soaked in the fish natural biological freshness-preserving agent after dilution, impregnate 20-30min, then fish taken out, is sealed.
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