CN106615074A - Composite biological fresh-keeping agent used for squid, and fresh-keeping method thereof - Google Patents
Composite biological fresh-keeping agent used for squid, and fresh-keeping method thereof Download PDFInfo
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- CN106615074A CN106615074A CN201611227143.1A CN201611227143A CN106615074A CN 106615074 A CN106615074 A CN 106615074A CN 201611227143 A CN201611227143 A CN 201611227143A CN 106615074 A CN106615074 A CN 106615074A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/08—Preserving with sugars
- A23B7/085—Preserving with sugars in a solution of sugar
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Abstract
The invention discloses a composite biological fresh-keeping agent used for squid, and a fresh-keeping method thereof. The composite biological fresh-keeping agent comprise a squid fresh-keeping agent 1 and a squid fresh-keeping agent 2; the squid fresh-keeping agent 1 and the squid fresh-keeping agent 2 are combined for fresh keeping of squid; the squid fresh-keeping agent 1 is prepared from enzymolysis products of flounder leftovers; the squid fresh-keeping agent 2 is prepared from laminarin, pine bark polyphenol extract, and resveratrol. The fresh-keeping method comprises following steps: fresh squid is washed; vesicle is removed; squid is cut into pieces, is uniformly immersed into the squid fresh-keeping agent 1 and the squid fresh-keeping agent 2 respectively, is taken out from the squid fresh-keeping agents, and is subjected to natural air drying at 4 DEG C; the obtained dried squid is stored in fresh-keeping bags; and the fresh-keeping bags are sealed, and are stored at 0 DEG C. The composite biological fresh-keeping agent also possesses antibacterial effect and oxidation resistance, and is capable of reducing formaldehyde content in squid, and prolonging self life of squid; synergistic effect of the composite biological fresh-keeping agent in maintaining of the quality of squid is achieved; and no obvious influence on the color of squid is caused.
Description
Technical field
The invention belongs to preservation of fishery field, is related to the complex biological preservative and its preservation method of a kind of squid.
Background technology
Squid, extra large mollush, Cephalopoda, fine and tender taste.Squid fat content is low, mainly phosphatide, is height insatiable hunger
And fat.Protein content is high in squid, and containing abundant amino acid, and essential amino acid proportion is very big.Also contain simultaneously
There are an elements such as a large amount of taurines, calcium, phosphorus, iron, and the trace element such as selenium, iodine, manganese, the copper of very abundant.Squid has regulation
Blood fat, reduces blood pressure, the effect such as anti-inflammatory, anti-oxidant and protection nerve cell.
Because squid institutional framework and physics and chemistry feature determine that it is easily putrid and deteriorated, make product quality be affected, reduce
Commodity value.Therefore, how to extend the preservation term of the squid flesh of fish, its wholesomeness is improved, in becoming whole squid industrial chain
A very important link, the preservation method of squid is always the popular class that aquatic products circle expert and aquatic products processing enterprise are inquired into
Topic.
For a long time, the preservation term of squid meat is extended more than people using freezing, although when squid can preserve very long
Between, but freezing, during storing, thawing, due to ice crystal formed and musculature in moisture recrystallization, albumen occurs cold
Freeze denaturation, the quality of squid meat and nutrition all there occurs larger change compared with Fresh squid's meat, and energy consumption is very high；Except cold
Outside jelly method, addition chemical preservative can also greatly prolong the commodity price phase of Fresh squid, but be excessively used preservative, strong to human body
Health harm is larger.
The content of the invention
It is an object of the invention to solve the problems, such as that the fresh-keeping security of squid meat chemical preservative is low, there is provided one kind has
Preferably water conservation, a kind of complex biological preservative of antibacterial, sterilized, antioxidant effect squid and its preservation method, can improve
The edible and commodity value of squid.
The present invention technical solution be：
A kind of complex biological preservative of squid, the complex biological preservative includes squid antistaling agent one and squid antistaling agent
Two, squid antistaling agent one and squid antistaling agent two are fresh-keeping with the use of carrying out to squid, the squid antistaling agent one it is effective into
It is divided into sole leftover bits and pieces zymolyte；The active ingredient of the squid antistaling agent two be laminarin, pine bark polyphenol extract and
Resveratrol.
Further, the sole leftover bits and pieces zymolyte mass concentration in the squid antistaling agent one is 7.5%～15%；Institute
The laminarin mass concentration stated in squid antistaling agent two is 0.1%～1.0%, and pine bark polyphenol extract mass concentration is
0.01%～0.2%, resveratrol mass concentration is 0.01%～0.2%, balance of water.
A kind of preservation method of squid, it is comprised the following steps that：
(1) preparation of sole leftover bits and pieces zymolyte
By sole leftover bits and pieces autoclaving, it is dried, mixes with water, the sole leftover bits and pieces is 1 with water mixing quality volume ratio:5
(g/mL), it is homogenized, obtains sole leftover bits and pieces mixed slurry, 40 DEG C of temperature regulating, adjust pH to be 7.0～8.0 with NaOH, adds pancreas
Enzyme is digested, and addition accounts for the pancreatin of sole leftover bits and pieces gross mass 0.5%～1.0%, and hydrolysis time is 4h, and go out enzyme, and centrifugation takes supernatant
Liquid, concentration, freeze-drying, obtains sole leftover bits and pieces zymolyte；
(2) preparation of laminarin
Raw material is sea-tangle powder, and using ultrasonic assistant laminarin is extracted；
(3) prepared by pine bark polyphenol extract
Korean pine bark is taken, is crushed with omnipotent bruisher, cross 30 mesh sieves, by Korean pine skin and the solid-liquid ratio 1 of ethanol solution:30(g/mL)
The ethanol solution of volumetric concentration 60% is added, is extracted in 50 DEG C of ultrasonic assistants, 20min, mistake are extracted under the conditions of ultrasonic power 200W
Filter, 55 DEG C are evaporated using Rotary Evaporators, obtain concentrate, with AB-8 type macroporous resin enrichment pine bark polyphenol, are entered with ethanol
Row gradient elution, concentration, vacuum freeze-drying, obtain Korean pine bark polyphenol freeze-dried powder, be placed in 4 DEG C it is standby；
(4) squid antistaling agent one is prepared
Sole leftover bits and pieces zymolyte is added water the solution for being configured to that mass concentration is 7.5%～15%, used as squid antistaling agent one；
(5) squid antistaling agent two is prepared
Resveratrol, laminarin and pine bark polyphenol extract are added in deionized water, is stirred, be configured to sea-tangle many
Sugared mass percent concentration is 0.1%～1.0%, and pine bark polyphenol extract mass percent concentration is 0.01%～0.2%,
Resveratrol mass percent concentration is 0.01%～0.2%, used as squid antistaling agent two；
(6) squid Preservation Treatment
New Fresh squid is cleaned, capsule is cut, sheet is then cut into, by squid chip in 0 DEG C of squid antistaling agent one and squid antistaling agent
It is uniform respectively in two to soak 30min～60min, squid is pulled out and drains 1min～2min, natural wind under the conditions of being placed in 4 DEG C
It is dry, the squid fillet after air-drying is put in freshness protection package, seal, it is placed on preservation in 0 DEG C of environment.
Further, temperature is 121 DEG C during autoclaving, and the time is 15min.
Further, baking temperature is 80 DEG C after autoclaving, and drying time is 2h.
Further, the sole leftover bits and pieces is fish head, fish-bone and the fish-skin of sole.
Further, when extracting laminarin with sea-tangle powder, first sea-tangle deionized water is cleaned up, at 105 DEG C
Lower drying 30min, by the sea-tangle of drying sea-tangle powder is worn into；Solid-liquid ratio according to sea-tangle powder and deionized water is 1:30
(g/mL) laminarin is extracted under ultrasonic assistant, is filtered, 55 DEG C are evaporated using Rotary Evaporators, the supernatant fluid that will be collected
Product is concentrated to the 1/5 of original volume, then by concentrate and absolute ethyl alcohol according to volume ratio 1:4～1:6 are well mixed, 4 DEG C of mistakes
Night；15min is centrifuged under 6000 turns/min revolutions, sediment is collected, is dried to constant weight at 55 DEG C, obtain laminarin.
Further, when step (1) is centrifuged, centrifugal force is 5000g, and centrifugation time is 15min.
Further, during step (2) ultrasonic wave extraction, ultrasonic wave sends the time for 2s, and the off time of ultrasonic wave is 2s,
Extraction all times are 50min, and ultrasonic power is 600W, 60 DEG C of Extracting temperature.
Beneficial effects of the present invention：
(1) sole leftover bits and pieces is easy to get, cheap, and protein content is high, is hydrolyzed into the micromolecule polypeptide of short chain, can release
More amino are released, the formaldehyde in squid is removed；Pancreatin is trypsase, chymotrypsin, elastoser, pancreas starch
The mixture of enzyme and pancreatic lipase, various enzyme effects discharge more amino captures in the differential responses site of sole leftover bits and pieces
Formaldehyde.Simultaneously sole leftover bits and pieces enzymolysis liquid also has certain water retention.
(2) resveratrol can suppress TMAOase active, reduce formaldehyde in squid and formed, and keep the product of squid protein
Matter；Sea-tangle and squid are all grown in the complicated seawater of chemical constituent, with identical physiological environment, with laminarin, pine tree
Skin polyphenol extract and resveratrol compound use, with more preferable antibacterial and antioxidant effect, when can extend the preservation of squid
Between.
(3) this complex biological preservative, had both inhibited the generation of formaldehyde in squid, and the first produced in squid is eliminated again
Aldehyde, this antistaling agent also has antibacterial and antioxidation, while holding of the complex biological preservative to Squid Quality has synergy
Effect, and the color and luster of sleeve-fish product is had no significant effect.
(4) squid antistaling agent preparation method is simple, and process is few, easy to use, only need to be with squid antistaling agent in low temperature
Lower immersion squid fillet can reach fresh-keeping purpose.
Specific embodiment
Embodiment 1
(1) preparation of sole leftover bits and pieces zymolyte
By sole leftover bits and pieces (fish head, bone, skin) autoclaving (121 DEG C, 15min), it is dried (80 DEG C, 2h), mixes with water and (get a foothold
Material compares 1 with water mixing quality:5 (g/mL)), homogenate, 40 DEG C of temperature regulating, it is 7.0 to adjust pH with NaOH, and addition accounts for sole leftover bits and pieces
The pancreatin of material gross mass 0.5%, hydrolysis time is 4h, and go out enzyme, is centrifuged (5000 × g, 15min), takes supernatant, concentration, freezing
It is dried, obtains sole leftover protein enzymolysis product；
(2) preparation of laminarin
Sea-tangle deionized water is cleaned up, at 105 DEG C 30min is dried, the sea-tangle of drying is worn into into sea-tangle powder, surpassed
Sound wave assisted extraction laminarin, ultrasonic wave sends the time for 2.0s, and the off time of ultrasonic wave is 2s, extracts all times and is
50min, ultrasonic power is 600W, 60 DEG C of Extracting temperature, and solid-liquid ratio is 1:30 (g/mL), filter, and at 55 DEG C rotary evaporation is adopted
Instrument evaporates, and the supernatant volume of collection is concentrated to into the 1/5 of original volume, by concentrate and absolute ethyl alcohol according to volume ratio 1:5
It is well mixed, 4 DEG C overnight；15min is centrifuged under 6000 turns/min revolutions, sediment is collected, 55 DEG C dry to constant weight, obtain sea
Band polysaccharide；
(3) prepared by pine bark polyphenol extract
50g Korean pine barks are taken, is crushed with omnipotent bruisher, crossed 30 mesh sieves and be placed in 2000mL beakers, by solid-liquid ratio 1:30(g/
ML) add the ethanol of volumetric concentration 60%, ultrasonic assistant to extract, at 50 DEG C, 20min, mistake are extracted under the conditions of ultrasonic power 200W
Filter, 55 DEG C are evaporated using Rotary Evaporators, obtain concentrate, with AB-8 type macroporous resin enrichment pine bark polyphenol, are entered with ethanol
Row gradient elution, concentration, vacuum freeze drier is freezed, and obtains Korean pine bark polyphenol freeze-dried powder, is placed in standby in 4 DEG C of refrigerators
With；
(4) antistaling agent one is prepared
Take sole leftover bits and pieces zymolyte to be well mixed with water, the solution that mass concentration is 7.5% is configured to, as antistaling agent one；
(5) antistaling agent two is prepared
Resveratrol, laminarin and pine bark polyphenol extract are added in deionized water, is stirred, be configured to sea-tangle many
Sugared mass percent concentration is 0.1%, and pine bark polyphenol extract percent concentration is 0.01%, resveratrol percent concentration
For 0.01%；(6) squid Preservation Treatment
New Fresh squid is cleaned, capsule is cut, sheet is then cut into, squid is neutralized in temperature for 0 DEG C of squid antistaling agent one fresh-keeping
It is uniform respectively in agent two to soak 30min, squid is pulled out and drains 1min, natural air drying under the conditions of being placed in 4 DEG C, after air-drying
Squid fillet is put in freshness protection package, sealing, is placed on preservation in 0 DEG C of environment.
Embodiment 2
(1) preparation of sole leftover bits and pieces zymolyte
By sole leftover bits and pieces (fish head, bone, skin) autoclaving (121 DEG C, 15min), it is dried (80 DEG C, 2h), mixes with water and (get a foothold
Material compares 1 with water mixing quality:5 (g/mL)), homogenate, 40 DEG C of temperature regulating, it is 7.5 to adjust pH with NaOH, and addition accounts for sole leftover bits and pieces
The pancreatin of material gross mass 0.7%, hydrolysis time is 4h, and go out enzyme, is centrifuged (5000 × g, 15min), takes supernatant, concentration, freezing
It is dried, obtains sole leftover protein enzymolysis product；
(2) preparation of laminarin
Sea-tangle deionized water is cleaned up, at 105 DEG C 30min is dried, the sea-tangle of drying is worn into into sea-tangle powder, surpassed
Sound wave assisted extraction laminarin, ultrasonic wave sends the time for 2.0s, and the off time of ultrasonic wave is 2s, extracts all times and is
50min, ultrasonic power is 600W, 60 DEG C of Extracting temperature, and solid-liquid ratio is 1:30 (g/mL), filter, and 55 DEG C adopt Rotary Evaporators
Evaporation, by the supernatant volume of collection the 1/5 of original volume is concentrated to, by concentrate and absolute ethyl alcohol according to volume ratio 1:4 mix
Close uniform, 4 DEG C overnight；15min is centrifuged under 6000 turns/min revolutions, sediment is collected, is dried to constant weight at 55 DEG C, obtain sea
Band polysaccharide；
(3) prepared by pine bark polyphenol extract
50g Korean pine barks are taken, is crushed with omnipotent bruisher, 30 mesh sieves are crossed, in being placed in 2000mL beakers, by solid-liquid ratio 1:30(g/
ML) add the ethanol of volumetric concentration 60%, ultrasonic assistant to extract, at 50 DEG C, 20min, mistake are extracted under the conditions of ultrasonic power 200W
Filter, 55 DEG C are evaporated using Rotary Evaporators, obtain concentrate, with AB-8 type macroporous resin enrichment pine bark polyphenol, are entered with ethanol
Row gradient elution, concentration, vacuum freeze drier vacuum freeze-drying obtains Korean pine bark polyphenol freeze-dried powder, in being placed in 4 DEG C of refrigerators
It is standby；
(4) antistaling agent one is prepared
Take sole leftover bits and pieces zymolyte to be well mixed with water, the solution that mass concentration is 10.0% is configured to, as antistaling agent one；
(5) antistaling agent two is prepared
Resveratrol, laminarin and pine bark polyphenol extract are added in deionized water, is stirred, be configured to sea-tangle many
Sugared mass percent concentration is 0.5%, and pine bark polyphenol extract mass percent concentration is 0.1%, resveratrol quality hundred
Specific concentration is divided to be 0.1%；
(6) squid Preservation Treatment
New Fresh squid is cleaned, capsule is cut, sheet is then cut into, in temperature is 0 DEG C of squid antistaling agent one and antistaling agent by squid
Uniform immersion 45min, squid is pulled out and drains 1.5min in two, natural air drying under the conditions of being placed in 4 DEG C, by the squid after air-drying
Sliced meat are put in freshness protection package, sealing, are placed on preservation in 0 DEG C of environment.
Embodiment 3
(1) preparation of sole leftover bits and pieces zymolyte
By sole leftover bits and pieces (fish head, bone, skin) autoclaving (121 DEG C, 15min), it is dried (80 DEG C, 2h), mixes with water and (get a foothold
Material compares 1 with water mixing quality:5 (g/mL)), homogenate, 40 DEG C of temperature regulating, it is 8.0 to adjust pH with NaOH, and addition accounts for sole leftover bits and pieces
The pancreatin of material gross mass 1.0%, hydrolysis time is 4h, and go out enzyme, is centrifuged (5000 × g, 15min), takes supernatant, concentration, freezing
It is dried, obtains sole leftover protein enzymolysis product；
(2) preparation of laminarin
Sea-tangle deionized water is cleaned up, at 105 DEG C 30min is dried, the sea-tangle of drying is worn into into sea-tangle powder, surpassed
Sound wave assisted extraction laminarin, ultrasonic wave sends the time for 2.0s, and the off time of ultrasonic wave is 2s, extracts all times and is
50min, ultrasonic power is 600W, 60 DEG C of Extracting temperature, and solid-liquid ratio is 1:30 (g/mL), filter, and at 55 DEG C rotary evaporation is adopted
The supernatant volume of collection is concentrated to into the 1/5 of original volume, by concentrate and absolute ethyl alcohol according to volume ratio 1:6 mixing are equal
Even, 4 DEG C overnight；6000 turns/min is centrifuged 15min, collects sediment, dries to constant weight at 55 DEG C, obtains laminarin；(3) pine
It is prepared by bark polyphenol extract
50g Korean pine barks are taken, is crushed with omnipotent bruisher, crossed 30 mesh sieves and be placed in 2000mL beakers, by solid-liquid ratio 1:30(g/
ML) add the ethanol of volumetric concentration 60%, ultrasonic assistant to extract, at 50 DEG C, 20min, mistake are extracted under the conditions of ultrasonic power 200W
Filter, 55 DEG C are evaporated using Rotary Evaporators, obtain concentrate, with AB-8 type macroporous resin enrichment pine bark polyphenol, are entered with ethanol
Row gradient elution, concentration, vacuum freeze drier is freezed, and obtains Korean pine bark polyphenol freeze-dried powder, is placed in standby in 4 DEG C of refrigerators
With；
(4) antistaling agent one is prepared
Take sole leftover bits and pieces zymolyte to be well mixed with water, the solution that mass concentration is 15.0% is configured to, as antistaling agent one；
(5) antistaling agent two is prepared
Resveratrol, laminarin and pine bark polyphenol extract are added in deionized water, is stirred, be configured to sea-tangle many
Sugared mass percent concentration is 1.0%, and pine bark polyphenol extract mass percent concentration is 0.2%, resveratrol quality hundred
Specific concentration is divided to be 0.2%；
(6) squid Preservation Treatment
New Fresh squid is cleaned, capsule is cut, sheet is then cut into, in temperature is 0 DEG C of squid antistaling agent one and antistaling agent by squid
Uniform immersion 60min, squid is pulled out and drains 2min in two, natural air drying under the conditions of being placed in 4 DEG C, by the squid meat after air-drying
Piece is put in freshness protection package, sealing, is placed on preservation in 0 DEG C of environment.
Comparative example 1
(1) preparation of sole leftover bits and pieces zymolyte
By sole leftover bits and pieces (fish head, bone, skin) autoclaving (121 DEG C, 15min), it is dried (80 DEG C, 2h), (sole is mixed with water
Leftover bits and pieces compares 1 with water mixing quality:5 (g/mL)), homogenate, 40 DEG C of temperature regulating, it is 7.5 to adjust pH with NaOH, and addition accounts for sole
The pancreatin of leftover bits and pieces gross mass 0.7%, hydrolysis time is 4h, and go out enzyme, is centrifuged (5000 × g, 15min), takes supernatant, concentrate,
Freeze-drying, obtains sole leftover protein enzymolysis product；
(2) antistaling agent is prepared
Take sole leftover bits and pieces zymolyte to be well mixed with water, the solution that mass concentration is 10.0% is configured to, as antistaling agent one；
(3) squid Preservation Treatment
New Fresh squid is cleaned, capsule is cut, sheet is then cut into, squid chip is uniformly soaked in temperature is 0 DEG C of squid antistaling agent
Bubble 45min, squid is pulled out and drains 1.5min, natural air drying under the conditions of being placed in 4 DEG C, and the squid fillet after air-drying is put into into guarantor
In fresh bag, sealing is placed on preservation in 0 DEG C of environment.
Comparative example 2
(1) preparation of sole leftover bits and pieces zymolyte
By sole leftover bits and pieces (fish head, bone, skin) autoclaving (121 DEG C, 15min), it is dried (80 DEG C, 2h), mixes with water and (get a foothold
Material compares 1 with water mixing quality:5 (g/mL)), homogenate, 40 DEG C of temperature regulating, it is 7.5 to adjust pH with NaOH, and addition accounts for sole leftover bits and pieces
The pancreatin of material gross mass 0.7%, go out enzyme, is centrifuged (5000 × g, 15min), takes supernatant, and concentration, freeze-drying obtain sole
Leftover protein enzymolysis product；
(2) antistaling agent one is prepared
Take sole leftover bits and pieces zymolyte to be well mixed with water, the solution that mass concentration is 10.0% is configured to, as antistaling agent one；
(3) antistaling agent two is prepared
Resveratrol is added in deionized water, is stirred, be configured to resveratrol percent concentration for 0.1%；
(4) squid Preservation Treatment
New Fresh squid is cleaned, capsule is cut, sheet is then cut into, in temperature is 0 DEG C of squid antistaling agent one and antistaling agent by squid
It is uniform respectively in two to soak 45min, squid is pulled out and drains 1.5min, natural air drying under the conditions of being placed in 4 DEG C, after air-drying
Squid fillet is put in freshness protection package, sealing, is placed on preservation in 0 DEG C of environment.
Comparative example 3
(1) preparation of sole leftover bits and pieces zymolyte
By sole leftover bits and pieces (fish head, bone, skin) autoclaving (121 DEG C, 15min), it is dried (80 DEG C, 2h), mixes with water and (get a foothold
Material compares 1 with water mixing quality:5 (g/mL)), homogenate, 40 DEG C of temperature regulating, it is 7.5 to adjust pH with NaOH, and addition accounts for sole leftover bits and pieces
The pancreatin of material gross mass 0.7%, hydrolysis time is 4h, and go out enzyme, is centrifuged (5000 × g, 15min), takes supernatant, concentration, freezing
It is dried, obtains sole leftover protein enzymolysis product；
(2) prepared by pine bark polyphenol extract
50g Korean pine barks are taken, is crushed with omnipotent bruisher, crossed 30 mesh sieves and be placed in 2000mL beakers, by solid-liquid ratio 1:30(g/
ML) add the ethanol of volumetric concentration 60%, ultrasonic assistant to extract, at 50 DEG C, 20min, mistake are extracted under the conditions of ultrasonic power 200W
Filter, 55 DEG C are evaporated using Rotary Evaporators, obtain concentrate, with AB-8 type macroporous resin enrichment pine bark polyphenol, are entered with ethanol
Row gradient elution, concentration, vacuum freeze drier is freezed, and obtains Korean pine bark polyphenol freeze-dried powder, is placed in standby in 4 DEG C of refrigerators
With；
(3) antistaling agent one is prepared
Take sole leftover bits and pieces zymolyte to be well mixed with water, the solution that mass concentration is 10.0% is configured to, as antistaling agent one；
(4) antistaling agent two is prepared
Resveratrol and pine bark polyphenol extract are added in deionized water, is stirred, be configured to many phenol extractions of pine bark
Thing percent concentration is 0.1%, and resveratrol percent concentration is 0.1%；
(5) squid Preservation Treatment
New Fresh squid is cleaned, capsule is cut, sheet is then cut into, in temperature is 0 DEG C of squid antistaling agent one and antistaling agent by squid
It is uniform respectively in two to soak 45min, squid is pulled out and drains 1.5min, natural air drying under the conditions of being placed in 4 DEG C, after air-drying
Squid fillet is put in freshness protection package, sealing, is placed on preservation in 0 DEG C of environment.
Comparative example 4
(1) preparation of sole leftover bits and pieces zymolyte
By sole leftover bits and pieces (fish head, bone, skin) autoclaving (121 DEG C, 15min), it is dried (80 DEG C, 2h), mixes with water and (get a foothold
Material compares 1 with water mixing quality:5 (g/mL)), homogenate, 40 DEG C of temperature regulating, it is 7.5 to adjust pH with NaOH, and addition accounts for sole leftover bits and pieces
The pancreatin of material gross mass 0.7%, hydrolysis time is 4h, and go out enzyme, is centrifuged (5000 × g, 15min), takes supernatant, concentration, freezing
It is dried, obtains sole leftover protein enzymolysis product；
(2) preparation of laminarin
Sea-tangle deionized water is cleaned up, at 105 DEG C 30min is dried, the sea-tangle of drying is worn into into sea-tangle powder, surpassed
Sound wave assisted extraction laminarin, ultrasonic wave sends the time for 2.0s, and the off time of ultrasonic wave is 2s, extracts all times and is
50min, ultrasonic power is 600W, 60 DEG C of Extracting temperature, and solid-liquid ratio is 1:30 (g/mL), filter, and 55 DEG C adopt Rotary Evaporators
Evaporation, by the supernatant volume of collection the 1/5 of original volume is concentrated to, by concentrate and absolute ethyl alcohol according to volume ratio 1:4 mix
Close uniform, 4 DEG C overnight；6000 turns/min is centrifuged 15min, collects sediment, and 55 DEG C dry to constant weight, obtain laminarin；
(3) antistaling agent one is prepared
Take sole leftover bits and pieces zymolyte to be well mixed with water, the solution that mass concentration is 10.0% is configured to, as antistaling agent one；
(4) antistaling agent two is prepared
Resveratrol, laminarin extract are added in deionized water, is stirred, be configured to laminarin mass percent
Concentration is 0.5%, and resveratrol percent concentration is 0.1%；
(5) squid Preservation Treatment
New Fresh squid is cleaned, capsule is cut, sheet is then cut into, squid is neutralized in temperature for 0 DEG C of squid antistaling agent one fresh-keeping
It is uniform respectively in agent two to soak 45min, squid is pulled out and drains 1.5min, natural air drying under the conditions of being placed in 4 DEG C, after air-drying
Squid fillet be put in freshness protection package, seal, be placed on preservation in 0 DEG C of environment.
Fresh-keeping effect
Control group replaces antistaling agent using sterilized water, and the squid handled well is put in PE freshness protection packages, in being stored in 0 DEG C of refrigerator,
Fresh-keeping effect is determined by the measure of TVB-N, total plate count and content of formaldehyde, as shown in table 1, table 2 and table 3.
The change unit (mg/100g) of squid storage period VBN (TVB-N) content of table 1
The change unit (lgCFU/g) of the squid storage period total plate count of table 2
The change unit (mg/kg) of the squid storage period content of formaldehyde of table 3
The squid processed using the antistaling agent of the present invention, in TVB-N, total plate count and content of formaldehyde control group is below, and is extended
Squid shelf life, laminarin, Korean pine bark polyphenol extract and resveratrol are used in mixed way the fresh-keeping effect to squid and are better than
Effect is used alone, they have synergistic function, and wherein embodiment 2 and embodiment 3 is best to squid fresh-keeping effect.Contrast
The content of formaldehyde of example 1 and comparative example 2 is substantially less than compareed, and is illustrated that sole leftover bits and pieces zymolyte and resveratrol have and is removed squid
Middle formaldehyde effect, comparative example 3, comparative example 4 and embodiment 1-3 content of formaldehyde are substantially less than comparative example 1 and comparative example 2, explanation
There is reciprocation in laminarin, Korean pine bark polyphenol extract and sole leftover bits and pieces zymolyte, resveratrol, remove formaldehyde effect
Fruit is better than the effect that sole leftover bits and pieces zymolyte and resveratrol are used alone.
The specific embodiment of the present invention is these are only, the present invention is not limited to, for those skilled in the art
For member, the present invention can have various modifications and variations.All any modifications within the spirit and principles in the present invention, made,
Equivalent, improvement etc., should be included within the scope of the present invention.
Claims (9)
1. a kind of complex biological preservative of squid, is characterized in that：The complex biological preservative includes the He of squid antistaling agent one
Squid antistaling agent two, squid antistaling agent one and squid antistaling agent two use cooperatively and fresh-keeping, the squid antistaling agent are carried out to squid
One active ingredient is sole leftover bits and pieces zymolyte；The active ingredient of the squid antistaling agent two is laminarin, pine bark is more
Phenol extraction thing and resveratrol.
2. the complex biological preservative of squid according to claim 1, is characterized in that：Flounder in the squid antistaling agent one
Fish pomace zymolyte mass concentration is 7.5%～15%；Laminarin mass concentration in the squid antistaling agent two is
0.1%～1.0%, pine bark polyphenol extract mass concentration is 0.01%～0.2%, and resveratrol mass concentration is 0.01%
～0.2%, balance of water.
3. a kind of preservation method of squid, is characterized in that：
Comprise the following steps that：
(1) preparation of sole leftover bits and pieces zymolyte
By sole leftover bits and pieces autoclaving, it is dried, mixes with water, the sole leftover bits and pieces is 1 with water mixing quality volume ratio:
5g/mL, homogenate, obtains sole leftover bits and pieces mixed slurry, 40 DEG C of temperature regulating, adjusts pH to be 7.0～8.0 with NaOH, adds pancreas
Enzyme is digested, and addition accounts for the pancreatin of sole leftover bits and pieces gross mass 0.5%～1.0%, and hydrolysis time is 4h, and go out enzyme, and centrifugation takes supernatant
Liquid, concentration, freeze-drying, obtains sole leftover bits and pieces zymolyte；
(2) preparation of laminarin
Raw material is sea-tangle powder, and using ultrasonic assistant laminarin is extracted；
(3) prepared by pine bark polyphenol extract
Korean pine bark is taken, is crushed with omnipotent bruisher, cross 30 mesh sieves, by Korean pine skin and the solid-liquid ratio 1 of ethanol solution:30g/mL adds
It is 60% ethanol solution to enter volumetric concentration, is extracted in 50 DEG C of ultrasonic assistants, and 20min, mistake are extracted under the conditions of ultrasonic power 200W
Filter, 55 DEG C adopt rotary evaporation, obtain concentrate, with AB-8 type macroporous resin enrichment pine bark polyphenol, with ethanol gradient are carried out
Wash-out, concentration, vacuum freeze-drying, obtain Korean pine bark polyphenol freeze-dried powder, be placed in 4 DEG C it is standby；
(4) squid antistaling agent one is prepared
Sole leftover bits and pieces zymolyte is added water the solution for being configured to that mass concentration is 7.5%～15%, used as squid antistaling agent one；
(5) squid antistaling agent two is prepared
Resveratrol, laminarin and pine bark polyphenol extract are added in deionized water, is stirred, be configured to sea-tangle many
Sugared mass percent concentration is 0.1%～1%, and pine bark polyphenol extract mass percent concentration is 0.01%～0.2%, in vain
Veratryl alcohol mass percent concentration is 0.01%～0.2%, used as squid antistaling agent two；
(6) squid Preservation Treatment
New Fresh squid is cleaned, capsule is cut, sheet is then cut into, squid is fresh-keeping in squid antistaling agent one and squid at 0 DEG C
It is uniform respectively in agent two to soak 30min～60min, squid is pulled out and drains 1min～2min, natural wind under the conditions of being placed in 4 DEG C
It is dry, the squid fillet after air-drying is put in freshness protection package, seal, it is placed on preservation in 0 DEG C of environment.
4. the preservation method of squid according to claim 3, is characterized in that：Temperature is 121 DEG C during autoclaving, and the time is
15min。
5. the preservation method of squid according to claim 3, is characterized in that：Baking temperature is 80 DEG C after autoclaving, is done
The dry time is 2h.
6. the preservation method of squid according to claim 3, is characterized in that：The sole leftover bits and pieces for sole fish head,
Fish-bone and fish-skin.
7. the preservation method of squid according to claim 3, is characterized in that：When extracting laminarin with sea-tangle powder, first
Sea-tangle deionized water is cleaned up, at 105 DEG C 30min is dried, the sea-tangle of drying is worn into into sea-tangle powder；According to sea
Solid-liquid ratio with powder and deionized water is 1:30g/mL extracts laminarin under ultrasonic assistant, filters, and 55 DEG C using rotation
Turn evaporimeter evaporation, the supernatant volume of collection be concentrated to into the 1/5 of original volume, then by concentrate and absolute ethyl alcohol according to
Volume ratio 1:4～1:6 are well mixed, and 4 DEG C overnight；15min is centrifuged under 6000 turns/min revolutions, sediment is collected, at 55 DEG C
Dry to constant weight, obtain laminarin.
8. the preservation method of squid according to claim 3, is characterized in that：When step (1) is centrifuged, centrifugal force is 5000g,
Centrifugation time is 15min.
9. the preservation method of squid according to claim 3, is characterized in that：During step (2) ultrasonic wave extraction, ultrasonic wave is sent out
Go out the time for 2s, off time of ultrasonic wave is 2s, extractions all times are 50min, ultrasonic power is 600W, Extracting temperature 60
℃。
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