CN109548855A - A kind of preparation method of the complex biological preservative of the extract containing pitaya peel - Google Patents
A kind of preparation method of the complex biological preservative of the extract containing pitaya peel Download PDFInfo
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- CN109548855A CN109548855A CN201910050768.2A CN201910050768A CN109548855A CN 109548855 A CN109548855 A CN 109548855A CN 201910050768 A CN201910050768 A CN 201910050768A CN 109548855 A CN109548855 A CN 109548855A
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- pitaya peel
- complex biological
- biological preservative
- salmon skin
- extract
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- 240000008086 Echinocereus enneacanthus Species 0.000 title claims abstract description 49
- 235000010837 Echinocereus enneacanthus subsp brevispinus Nutrition 0.000 title claims abstract description 49
- 235000006850 Echinocereus enneacanthus var dubius Nutrition 0.000 title claims abstract description 49
- 239000000284 extract Substances 0.000 title claims abstract description 48
- 239000003755 preservative agent Substances 0.000 title claims abstract description 26
- 230000002335 preservative effect Effects 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 241000972773 Aulopiformes Species 0.000 claims abstract description 30
- 235000019515 salmon Nutrition 0.000 claims abstract description 30
- 235000001808 Ceanothus spinosus Nutrition 0.000 claims abstract description 23
- 241001264786 Ceanothus spinosus Species 0.000 claims abstract description 23
- 108010010803 Gelatin Proteins 0.000 claims abstract description 22
- 229920000159 gelatin Polymers 0.000 claims abstract description 22
- 239000008273 gelatin Substances 0.000 claims abstract description 22
- 235000019322 gelatine Nutrition 0.000 claims abstract description 22
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 22
- 108010039918 Polylysine Proteins 0.000 claims abstract description 11
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 7
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 6
- 239000012498 ultrapure water Substances 0.000 claims abstract description 6
- 238000004090 dissolution Methods 0.000 claims abstract description 5
- 238000000605 extraction Methods 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 18
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 12
- 238000007654 immersion Methods 0.000 claims description 11
- 239000012141 concentrate Substances 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 244000157072 Hylocereus undatus Species 0.000 claims description 7
- 235000018481 Hylocereus undatus Nutrition 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 4
- 238000001704 evaporation Methods 0.000 claims description 4
- 230000008020 evaporation Effects 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 238000002390 rotary evaporation Methods 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 241000143060 Americamysis bahia Species 0.000 claims 1
- 241000238557 Decapoda Species 0.000 abstract description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 6
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 3
- 231100000331 toxic Toxicity 0.000 abstract description 2
- 230000002588 toxic effect Effects 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 238000004321 preservation Methods 0.000 description 5
- 230000000052 comparative effect Effects 0.000 description 4
- 239000002585 base Substances 0.000 description 3
- 239000006227 byproduct Substances 0.000 description 2
- 230000001934 delay Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 150000008442 polyphenolic compounds Chemical class 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
A kind of preparation method of the complex biological preservative of the extract containing pitaya peel, antistaling agent include salmon skin gelatin, red heart pitaya peel extract and epsilon-polylysine.Salmon skin gelatin is extracted using the salmon skin frozen at -20 DEG C, pitaya peel extract is extracted using red heart pitaya peel, every 100ml ultrapure water dissolution 1.2g salmon skin gelatin and 0.1g epsilon-polylysine form solution, then 2%~3% pitaya peel extract is added, and obtains complex biological preservative with magnetic stirrer 90min at normal temperature.Complex biological preservative of the invention has good fresh-keeping effect, and raw material is natural, has no toxic side effect, and can effectively delay the increase of total plate count and Volatile Base Nitrogen, and then extend the freshness date of peeled shrimp.
Description
Technical field
The present invention relates to technical field of aquatic product preservation, and in particular to a kind of red heart pitaya peel extract compound bio guarantor
Fresh dose.
Background technique
Pitaya peel is often discarded as the by-product that pulp is processed, and is developed and used, is turned waste into wealth to it, both may be used
Resource utilization is improved, dragon fruit added value can be improved again conducive to environmental protection.Nutriment is abundant in pitaya peel in fact, not only rich
Containing a variety of organic acids, protein, also containing functional components such as the rare pigment of general plant, polyphenol, and pitaya peel is more
Phenol has resistance of wide spectrum to microorganism, shows good bacteriostasis.So pitaya peel has very in preservation of fishery
Big potentiality.
Cray protein content is high, and fat content is low, soft, easy to digest.It is edible that it is that one kind is very suitable to the mankind
Food.In recent years, cray has become the important economic kind of China.But cray water activity is high, and full of nutrition, itself takes
It is easily putrid and deteriorated with a large amount of microorganism.In recent years, some with Nantural non-toxic, the water made of the compound of extract
Product preservation technology gradually causes the concern of people, and starts processing, storage, transport and the process of consumption applied to aquatic products
In.
Therefore, the invention proposes a kind of preparation of red heart pitaya peel extract complex biological preservative and its users
Method.
Summary of the invention
The purpose of the present invention is to provide using the by-product pitaya peel processed as pulp, a kind of red heart flue is prepared
The method of pericarp extract complex biological preservative.
The present invention is short for peeled shrimp fresh keeping time and causes easily putrid and deteriorated and influences edible problem, provides a kind of red heart
Pitaya peel extract complex biological preservative, extends the shelf-life of peeled shrimp.
The technical solution of the present invention is as follows: antistaling agent is by salmon skin gelatin, red heart pitaya peel extract, epsilon-polylysine
It is compound to be made;Antistaling agent is made by following steps: (1) prepared by salmon skin gelatin powder: taking three texts frozen at -20 DEG C
Fish-skin, thaw 20h under 4 DEG C of refrigerated conditions, then is cleaned with water, is shredded after cleaning again with 0.2Mol NaoH solution 4
DEG C 1h is impregnated, solid-liquid ratio is 1:6 when immersion;It is rinsed with water to neutrality, then impregnates 1h with 0.05N acetum, feed liquid when immersion
Than for 1:6;It is rinsed with water later to neutrality, then the salmon skin shredded is extracted into 3h under 60 DEG C of hot water, solid-liquid ratio when extraction
It for 1:6, is filtered after extraction with double gauze, filtrate is concentrated by evaporation with Rotary Evaporators;Concentrate is finally placed in -80 DEG C
Refrigerator kind prefreezing 10 minutes, then be placed in 10 DEG C of freeze dryers and be lyophilized 48 hours, obtain salmon skin gelatin;Salmon skin is bright
Glue grind into powder, for use;
(2) prepared by pitaya peel extract: red heart dragon fruit pericarp is extracted with 80% methanol, solid-liquid ratio is 4 when extraction:
1, extraction temperature is 40 DEG C, extraction time 90min, extracts series 2;Extracting solution is filtered with whatmann1 filter paper, filters it
Concentrate is finally being placed in -80 DEG C of refrigerator kind prefreezings 10 minutes, then is being placed in 10 DEG C of freeze dryers and is lyophilized 48 by concentrated by rotary evaporation afterwards
Hour, pitaya peel extract is obtained, for use;
(3) every 100ml ultrapure water dissolution 1.2g salmon skin gelatin and 0.1g epsilon-polylysine form solution, then add 2%
~3% pitaya peel extract, and magnetic stirrer 90min is being used at normal temperature, obtain complex biological preservative.
The present invention is short for peeled shrimp fresh keeping time and causes easily putrid and deteriorated and influences edible problem, provides a kind of red heart
Pitaya peel extract complex biological preservative extends the shelf-life of peeled shrimp, and the technical solution of used application is as follows:
New fresh shrimp is taken, is put into red heart pitaya peel extract Multiple preservative solutions and impregnates 30s.It pulls out and drains, be packed into sterile appearance
It is sealed in device, is finally placed under 4 DEG C of environment and refrigerates.
The present invention prepares the fresh-keeping liquid for preservation of fishery by raw material of pitaya peel, is pure natural fresh-keeping liquid,
It is nontoxic without influence and preferable to the fresh-keeping effect of aquatic products on human health, effectively delay total plate count and volatility alkali
The increase of nitrogen, and then the freshness date for extending peeled shrimp can also carry out pitaya peel on the basis of promoting aquatic products commodity value
Deep development utilizes, so that the application for further exploitation pitaya peel in preservation of fishery field provides theoretical foundation.
Detailed description of the invention
Fig. 1 is embodiment 3, total plate count of the sample in storage changes in embodiment 4, comparative example 5.
Fig. 2 is embodiment 3, Volatile Base Nitrogen (TVB-N) of the sample in storage becomes in embodiment 4, comparative example 5
Change.
Specific embodiment
In order to preferably disclose the present invention, explanation is further explained to the present invention combined with specific embodiments below.
Embodiment 1
A kind of red heart pitaya peel extract complex biological preservative is mainly extracted by salmon skin gelatin, red heart pitaya peel
Object, epsilon-polylysine are made.
A kind of complex biological preservative of the extract containing pitaya peel is made by following steps: (1) salmon skin gelatin
Powder preparation: taking the salmon skin frozen at -20 DEG C, and thaw 20h under 4 DEG C of refrigerated conditions, then is cleaned with water, cleans
Shredding later and using 0.2Mol NaoH solution solid-liquid ratio in 4 DEG C of immersion 1h, immersion again is 1:6;It is rinsed with water to neutrality, then uses
0.05N acetum impregnates 1h, and solid-liquid ratio is 1:6 when immersion;It is rinsed with water later to neutrality, then the salmon skin shredded is existed
Extract 3h under 60 DEG C of hot water, solid-liquid ratio is 1:6 when extraction, is filtered after extraction with double gauze, by filtrate Rotary Evaporators
It is concentrated by evaporation;Concentrate is finally placed in -80 DEG C of refrigerator kind prefreezings 10 minutes, then to be placed in 10 DEG C of freeze dryers freeze-drying 48 small
When, obtain salmon skin gelatin;By salmon skin gelatin grind into powder, for use;
(2) prepared by pitaya peel extract: red heart dragon fruit pericarp is extracted with 80% methanol, solid-liquid ratio is 4 when extraction:
1, extraction temperature is 40 DEG C, extraction time 90min, extracts series 2;Extracting solution is filtered with whatmann1 filter paper, filters it
Concentrate is finally being placed in -80 DEG C of refrigerator kind prefreezings 10 minutes, then is being placed in 10 DEG C of freeze dryers and is lyophilized 48 by concentrated by rotary evaporation afterwards
Hour, pitaya peel extract is obtained, for use;
(3) every 100ml ultrapure water dissolution 1.2g salmon skin gelatin and 0.1g epsilon-polylysine form solution, then add 2%
Pitaya peel extract, and magnetic stirrer 90min is being used at normal temperature, obtain complex biological preservative.
Embodiment 2
A kind of red heart pitaya peel extract complex biological preservative is mainly extracted by salmon skin gelatin, red heart pitaya peel
Object, epsilon-polylysine are made.
A kind of complex biological preservative of the extract containing pitaya peel is made by following steps: (1) salmon skin gelatin
Powder preparation: taking the salmon skin frozen at -20 DEG C, and thaw 20h under 4 DEG C of refrigerated conditions, then is cleaned with water, cleans
Shredding later and using 0.2Mol NaoH solution solid-liquid ratio in 4 DEG C of immersion 1h, immersion again is 1:6;It is rinsed with water to neutrality, then uses
0.05N acetum impregnates 1h, and solid-liquid ratio is 1:6 when immersion;It is rinsed with water later to neutrality, then the salmon skin shredded is existed
Extract 3h under 60 DEG C of hot water, solid-liquid ratio is 1:6 when extraction, is filtered after extraction with double gauze, by filtrate Rotary Evaporators
It is concentrated by evaporation;Concentrate is finally placed in -80 DEG C of refrigerator kind prefreezings 10 minutes, then to be placed in 10 DEG C of freeze dryers freeze-drying 48 small
When, obtain salmon skin gelatin;By salmon skin gelatin grind into powder, for use;
(2) prepared by pitaya peel extract: red heart dragon fruit pericarp is extracted with 80% methanol, solid-liquid ratio is 4 when extraction:
1, extraction temperature is 40 DEG C, extraction time 90min, extracts series 2;Extracting solution is filtered with whatmann1 filter paper, filters it
Concentrate is finally being placed in -80 DEG C of refrigerator kind prefreezings 10 minutes, then is being placed in 10 DEG C of freeze dryers and is lyophilized 48 by concentrated by rotary evaporation afterwards
Hour, pitaya peel extract is obtained, for use;
(3) every 100ml ultrapure water dissolution 1.2g salmon skin gelatin and 0.1g epsilon-polylysine form solution, then add 3%
Pitaya peel extract, and magnetic stirrer 90min is being used at normal temperature, obtain complex biological preservative.
Embodiment 3
The application method of complex biological preservative is carried out according to the following steps: taking new fresh shrimp, is put into and extracts containing 2% dragon fruit
30s is impregnated in the red heart pitaya peel extract complex biological preservative of object.It pulls out and drains, be fitted into sterile chamber and seal, most
It is placed under 4 DEG C of environment and refrigerates.
Embodiment 4
The application method of complex biological preservative is carried out according to the following steps: taking new fresh shrimp, is put into and extracts containing 3% dragon fruit
30s is impregnated in the red heart pitaya peel extract complex biological preservative of object.It pulls out and drains, be fitted into sterile chamber and seal, most
It is placed under 4 DEG C of environment and refrigerates.
5 comparative example of embodiment
New fresh shrimp is taken, is put into ultrapure water and impregnates 30s.It pulls out and drains, be fitted into sterile chamber and seal, be finally placed in 4 DEG C of rings
It is refrigerated under border.
By attached drawing 1,2 it is found that a kind of red heart pitaya peel extract complex biological preservative can effectively delay bacterium
Fall the increase of sum and Volatile Base Nitrogen.The edible upper limit of total plate count is 7log CFU/g in aquatic products, low initial
Total plate count (5.28 ± 0.21 log CFU/g) show that peeled shrimp possesses relatively good quality;It is right by the 4th day of storage
Ratio 5 increases to 7.88 ± 0.29CFU/g, and embodiment 3 and embodiment 4 are still below 7log10CFU/g;Two days later, real
The total plate count for applying example 3 and embodiment 4 has also exceeded 7 log10 CFU/g.The edible upper limit of TVB-N is in aquatic products
30mg / 100g.It is 4.58mg/100g in the TVB-N value of the 0th day shrimp;By the 6th day of Storage period, the TVB-N of comparative example 5
Value reaches inedible level (31.13mg/100g);Reach inedible water in the 8th day embodiment 3 and embodiment 4
It is flat.Pitaya peel extract has certain antibacterial activity and effectively delays the increase of TVB-N, and then extends the fresh-keeping of peeled shrimp
Phase.
The beneficial effects of the present invention are: being formed using salmon skin, red heart pitaya peel and epsilon-polylysine, have good
Fresh-keeping effect, raw material is natural, has no toxic side effect, and effectively delays the increase of total plate count and Volatile Base Nitrogen, and then extend
The freshness date of peeled shrimp.
The above described is only a preferred embodiment of the present invention, being not the limit for making any other form to the present invention
System, and made any modification or equivalent variations according to the technical essence of the invention, still fall within present invention model claimed
It encloses.
Claims (3)
1. a kind of preparation method of the complex biological preservative of extract containing pitaya peel, which is characterized in that antistaling agent is by three texts
Fishskin gelatin, red heart pitaya peel extract, epsilon-polylysine are compound to be made;Antistaling agent is made by following steps: (1) three text
The preparation of fishskin gelatin powder: the salmon skin frozen at -20 DEG C is taken, thaw 20h under 4 DEG C of refrigerated conditions, then is carried out with water
Cleaning, shredding after cleaning and using 0.2Mol NaoH solution solid-liquid ratio in 4 DEG C of immersion 1h, immersion again is 1:6;Be rinsed with water to
Neutrality, then 1h is impregnated with 0.05N acetum, solid-liquid ratio is 1:6 when immersion;It is rinsed with water to neutrality, then will shred later
Salmon skin extracts 3h under 60 DEG C of hot water, and solid-liquid ratio is 1:6 when extraction, is filtered after extraction with double gauze, filtrate is used
Rotary Evaporators are concentrated by evaporation;Concentrate is finally placed in -80 DEG C of refrigerator kind prefreezings 10 minutes, then is placed in 10 DEG C of freeze dryers
Freeze-drying 48 hours, obtains salmon skin gelatin;By salmon skin gelatin grind into powder, for use;
(2) prepared by pitaya peel extract: red heart dragon fruit pericarp is extracted with 80% methanol, solid-liquid ratio is 4 when extraction:
1, extraction temperature is 40 DEG C, extraction time 90min, extracts series 2;Extracting solution is filtered with whatmann1 filter paper, filters it
Concentrate is finally being placed in -80 DEG C of refrigerator kind prefreezings 10 minutes, then is being placed in 10 DEG C of freeze dryers and is lyophilized 48 by concentrated by rotary evaporation afterwards
Hour, pitaya peel extract is obtained, for use;
(3) every 100ml ultrapure water dissolution 1.2g salmon skin gelatin and 0.1g epsilon-polylysine form solution, then add 2%
~3% pitaya peel extract, and magnetic stirrer 90min is being used at normal temperature, obtain complex biological preservative.
2. a kind of preparation method of the complex biological preservative of extract containing pitaya peel, which is characterized in that the compound bio
The content of red heart pitaya peel extract is 3% in antistaling agent.
3. a kind of preparation method of the complex biological preservative of extract containing pitaya peel, which is characterized in that the compound bio
The fresh-keeping object of antistaling agent is shrimps.
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CN201910050768.2A CN109548855A (en) | 2019-01-20 | 2019-01-20 | A kind of preparation method of the complex biological preservative of the extract containing pitaya peel |
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Family
ID=65873354
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Cited By (1)
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CN114403210A (en) * | 2022-01-28 | 2022-04-29 | 新疆农垦科学院 | Fresh jujube normal temperature circulation special-purpose fresh-keeping liquid and preparation method and application thereof |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN114403210A (en) * | 2022-01-28 | 2022-04-29 | 新疆农垦科学院 | Fresh jujube normal temperature circulation special-purpose fresh-keeping liquid and preparation method and application thereof |
CN114403210B (en) * | 2022-01-28 | 2023-08-22 | 新疆农垦科学院 | Special fresh-keeping liquid for normal-temperature circulation of fresh jujube, and preparation method and application thereof |
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