CN109673587A - A kind of preparation method for the transparent earth materials can be used for soil animal movable observing - Google Patents
A kind of preparation method for the transparent earth materials can be used for soil animal movable observing Download PDFInfo
- Publication number
- CN109673587A CN109673587A CN201811473457.9A CN201811473457A CN109673587A CN 109673587 A CN109673587 A CN 109673587A CN 201811473457 A CN201811473457 A CN 201811473457A CN 109673587 A CN109673587 A CN 109673587A
- Authority
- CN
- China
- Prior art keywords
- culture medium
- earth materials
- fish meal
- soil
- transparent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000002689 soil Substances 0.000 title claims abstract description 56
- 241001465754 Metazoa Species 0.000 title claims abstract description 26
- 239000000463 material Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title abstract description 6
- 239000001963 growth medium Substances 0.000 claims abstract description 36
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 32
- 108010080698 Peptones Proteins 0.000 claims abstract description 32
- 239000004467 fishmeal Substances 0.000 claims abstract description 32
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims abstract description 17
- 229920002472 Starch Polymers 0.000 claims abstract description 17
- 235000019698 starch Nutrition 0.000 claims abstract description 17
- 239000008107 starch Substances 0.000 claims abstract description 17
- 239000011347 resin Substances 0.000 claims abstract description 11
- 229920005989 resin Polymers 0.000 claims abstract description 11
- 229920000881 Modified starch Polymers 0.000 claims abstract description 5
- 239000004368 Modified starch Substances 0.000 claims abstract description 5
- 239000002253 acid Substances 0.000 claims abstract description 5
- 235000019426 modified starch Nutrition 0.000 claims abstract description 5
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 claims abstract description 5
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 claims abstract description 5
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 claims description 15
- 239000001888 Peptone Substances 0.000 claims description 10
- 235000019319 peptone Nutrition 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 230000007774 longterm Effects 0.000 claims description 7
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 239000011259 mixed solution Substances 0.000 claims description 4
- 239000002537 cosmetic Substances 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 11
- 239000004925 Acrylic resin Substances 0.000 abstract description 2
- 241000361919 Metaphire sieboldi Species 0.000 description 22
- 239000002245 particle Substances 0.000 description 11
- 230000008961 swelling Effects 0.000 description 8
- 230000001954 sterilising effect Effects 0.000 description 6
- 230000000052 comparative effect Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000005303 weighing Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 238000002591 computed tomography Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000002242 deionisation method Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000019837 monoammonium phosphate Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 description 1
- 239000004324 sodium propionate Substances 0.000 description 1
- 235000010334 sodium propionate Nutrition 0.000 description 1
- 229960003212 sodium propionate Drugs 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of preparation methods of transparent earth materials that can be used for soil animal movable observing；The transparent earth materials are that modified starch grafted propylene acid resin is added in the Ms culture medium containing fish meal protein peptone to be swollen；It is described to be modified as starch graft acrylic acid resin being crushed to granularity being 0.5~1mm.The transparent earth materials that can be used for soil animal movable observing for a long time can be made in the present invention in cheap, quick method.
Description
Technical field
The invention belongs to agricultural technology fields, and in particular to a kind of preparation method of transparent earth materials, the material of preparation can
Observation for soil animal behavior.
Background technique
Soil is the tie for linking up water body and atmosphere, is one of constituent important in the ecosystem.It lives in soil
Numerous animals, plant and microorganism, these biology and soil between influence each other, can by biology animation it is fast
Speed judges soil quality.The destructive sampling of progress is generally required to the activity condition of soil animal before, separating soil can just see
It arrives.Also have through certain technological means, as CT scan is realized；But this method is complicated, and cost is excessively high, and being unfavorable for promoting makes
With.It is retrieved, discovery has the patent [application number 201611000726.0] that plant culture is used for using half seep through, the patent readme
For " the invention discloses a kind of translucent plants to cultivate glue, is made of the following components according to parts by weight: polyamide, boric acid
Powder, sodium nitrate, magnesium sulfate, ammonium sulfate, castor oil, copper sulphate, ammonium dihydrogen phosphate, sodium propionate, appropriate amount of water ", the patent can be straight
Observe the overall process that plant slowly germinates with seeing.But the patent composition is complicated, soil animal such as earthworm are deposited in the soil
Live time is shorter, through testing, not can be carried out long-term observation.Reason may the culture medium carbon-nitrogen ratio or certain ingredients be not suitable for soil
The existence of earth Long term Animal.And having coloured substance containing copper sulphate etc. in the glue of its production, transparency is not high, for soil
The moving process observing effect of animal is bad.
Summary of the invention
It is an object of the invention to solve the problems mentioned above in the background art, soil can directly be observed by providing one kind
The preparation method of the transparent earth materials of animal behavior variation；Transparent soil can be made in cheap, quick method in the present invention
Material, it is simple and convenient, it is easy to operate.
The purpose of the present invention is what is be achieved through the following technical solutions:
The present invention relates to a kind of transparent earth materials, the transparent earth materials are by modified starch grafted propylene acid resin
It is added in the Ms culture medium of the peptone containing fish meal protein and is swollen.
Preferably, described to be modified as starch graft acrylic acid resin being crushed to granularity being 0.5~1mm.If diameter is less than
0.5mm, after the later period is swollen, particle diameter is too small, and porosity can become smaller, and is not easy to animal survival；Particle diameter is greater than
1mm, postmenstruation swelling, particle diameter is excessive, and material shows to will form various optical refractions, reflection etc., and transparency reduces, shadow
Ring observation.
Preferably, the Ms culture medium of the peptone containing fish meal protein, for deionization is added in fish meal protein peptone and Ms culture medium
In water, wherein the content of fish meal protein peptone is 25~35g/L, and the content of Ms culture medium is 3~5g/L.By mixture at 121 DEG C
20~30min of lower sterilizing and obtain.When fish meal protein peptone content is lower than 25g/L and is higher than 35g/L, whole carbon nitrogen cannot be in
The range of one suitable soil animal existence, influences the long-term life of animal.When Ms culture medium content is lower than 3g/L, resin is molten
After swollen, show to will appear flakes, is unfavorable for observing；When culture medium content is higher than 5g/L, after resin swelling, porosity compared with
Small, dissolved oxygen is insufficient, is unfavorable for soil animal long term survival.
Preferably, the mass volume ratio of the modified starch grafted propylene acid resin and the Ms culture medium of the peptone containing fish meal protein
For 1:30~1:45g/mL.When mass volume ratio is less than 1:30, material swelling is insufficient, shows to have some materials swelling endless
Entirely, the existence of animal is influenced.When mass volume ratio is greater than 1:45, after being swollen, having some materials has mobility, is unfavorable for moving
The movable observation of object.
Preferably, the transparent earth materials are for the long-term observation of soil animal activity.
Compared with prior art, the invention has the following beneficial effects:
1, the present invention, can by modification using cheap starch graft acrylic acid, fish meal protein peptone and Ms culture medium as raw material
For observing the behavioral activity of soil animal.
2, the present invention using starch graft acrylic acid, fish meal protein peptone and Ms culture medium as raw material, to soil animal toxicity compared with
It is small, it can be used in the long-term observation of soil animal behavior.
3, material according to the present invention is prepared simple and fast, economic and practical.
Specific embodiment
The following describes the present invention in detail with reference to examples.Following embodiment will be helpful to those skilled in the art
The present invention is further understood, but the invention is not limited in any way.It should be pointed out that those skilled in the art
For, without departing from the inventive concept of the premise, it can also make certain adjustments and improvements.These belong to guarantor of the invention
Protect range.
Embodiment 1
The dry starch graft acrylic acid particle of 10g is weighed, is crushed in pulverizer, finally reaches its granularity
1mm, after mixing for use；Later fish meal protein peptone and Ms culture medium are added in deionized water, wherein fish meal protein peptone
Content is 30g/L, and the content of Ms culture medium is 4g/L, and the mixed solution is at 121 DEG C, and sterilize 30min, obtains containing fish meal protein
The Ms culture medium of peptone；Later by the mass volume ratio of starch graft acrylic acid and the Ms culture medium containing fish meal protein peptone of sterilizing
For 1:35, the starch graft acrylic acid particle for weighing corresponding mass is put into the Ms culture medium containing fish meal protein peptone of corresponding volume
In, after resin complete swelling, obtain transparent soil.The soil animals such as earthworm are put into the transparent soil, can visually be seen
To the active procedure of earthworm in the soil, until termination test after two months, earthworm normal existence also in the soil.Illustrate soil
Earth earthworm can be lived in for a long time in the transparent soil.
Embodiment 2
The dry starch graft acrylic acid particle of 10g is weighed, is crushed in pulverizer, finally reaches its granularity
0.5mm, after mixing for use；Fish meal protein peptone and Ms culture medium are added in deionized water later, wherein fish meal protein peptone
Content be 25g/L, the content of Ms culture medium is 3g/L, and the mixed solution is at 121 DEG C, and sterilize 20min, obtains containing fish meal egg
The Ms culture medium of white peptone；Later by the quality volume of starch graft acrylic acid and the Ms culture medium containing fish meal protein peptone of sterilizing
Than for 1:30, the starch graft acrylic acid particle for weighing corresponding mass is put into the Ms culture containing fish meal protein peptone of corresponding volume
In base, after resin complete swelling, transparent soil is obtained.The soil animals such as earthworm are put into the transparent soil, it can naked eyes
The active procedure of earthworm in the soil is seen, until termination test after two months, normal existence of the earthworm also in the soil.It says
Bright soil earthworm can be lived in for a long time in the transparent soil.
Embodiment 3
The dry starch graft acrylic acid particle of 10g is weighed, is crushed in pulverizer, finally reaches its granularity
0.8mm, after mixing for use；Fish meal protein peptone and Ms culture medium are added in deionized water later, wherein fish meal protein peptone
Content be 35g/L, the content of Ms culture medium is 5g/L, and the mixed solution is at 121 DEG C, and sterilize 25min, obtains containing fish meal egg
The Ms culture medium of white peptone；Later by the quality volume of starch graft acrylic acid and the Ms culture medium containing fish meal protein peptone of sterilizing
Than for 1:45, the starch graft acrylic acid particle for weighing corresponding mass is put into the Ms culture containing fish meal protein peptone of corresponding volume
In base, after resin complete swelling, transparent soil is obtained.The soil animals such as earthworm are put into the transparent soil, it can naked eyes
The active procedure of earthworm in the soil is seen, until termination test after two months, normal existence of the earthworm also in the soil.It says
Bright soil earthworm can be lived in for a long time in the transparent soil.
Comparative example 1
The dry starch graft acrylic acid particle of 10g is weighed, is crushed in pulverizer, finally reaches its granularity
1mm, after mixing for use；Ms culture medium is added in deionized water later, the content of Ms culture medium is 4g/L, then 121
DEG C, sterilize 30min, the Ms culture medium to be sterilized；Later by the mass body of starch graft acrylic acid and the Ms culture medium of sterilizing
Product is than being 1:35, in the Ms culture medium for the sterilizing that the starch graft acrylic acid particle for weighing corresponding mass is put into corresponding volume, to
After resin complete swelling, transparent soil is obtained.The soil animals such as earthworm are put into the transparent soil, can visually see earthworm
Active procedure in the soil.But after two weeks, earthworm survival condition be deteriorated, mobility weaken, have a small amount of yellow liquid from
Body surface exudation.Illustrate that soil earthworm cannot be lived in for a long time in the transparent soil.
Comparative example 2
This comparative example substantially with embodiment 1, institute the difference is that: provide nitrogen source with ammonium sulfate substitution fish meal protein peptone.
The soil animals such as earthworm are put into the transparent soil of the comparative example, can visually see the active procedure of earthworm in the soil,
But after two weeks, earthworm movement reduced capability, earthworm band portions enlargement, portion link curling.Illustrate that soil earthworm cannot
It is lived in the transparent soil for a long time.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited to above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring substantive content of the invention.
Claims (6)
1. a kind of transparent earth materials, which is characterized in that the transparent earth materials are by modified starch grafted propylene acid resin
It is added in the Ms culture medium of the peptone containing fish meal protein and is swollen.
2. transparent earth materials according to claim 1, which is characterized in that described to be modified as starch graft acrylic acid tree
It is 0.5~1mm that cosmetics, which is broken to granularity,.
3. transparent earth materials according to claim 1, which is characterized in that the Ms culture medium of the peptone containing fish meal protein is
Fish meal protein peptone and Ms culture medium are added in deionized water, wherein the content of fish meal protein peptone is 25~35g/L, Ms culture medium
Content be 3~5g/L.
4. transparent earth materials according to claim 3, which is characterized in that fish meal protein peptone and Ms culture medium addition go from
Mixed solution after sub- water sterilizes 20~30min at 121 DEG C to get the Ms culture medium of the peptone containing fish meal protein.
5. transparent earth materials according to claim 1, which is characterized in that the modified starch grafted propylene acid resin with
The mass volume ratio of the Ms culture medium of the peptone containing fish meal protein is 1:30~1:45g/mL.
6. transparent earth materials as described in claim 1, which is characterized in that the transparent earth materials are living for soil animal
Dynamic long-term observation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811473457.9A CN109673587B (en) | 2018-12-04 | 2018-12-04 | Preparation method of transparent soil material for soil animal activity observation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811473457.9A CN109673587B (en) | 2018-12-04 | 2018-12-04 | Preparation method of transparent soil material for soil animal activity observation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109673587A true CN109673587A (en) | 2019-04-26 |
| CN109673587B CN109673587B (en) | 2020-07-24 |
Family
ID=66186910
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811473457.9A Active CN109673587B (en) | 2018-12-04 | 2018-12-04 | Preparation method of transparent soil material for soil animal activity observation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109673587B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283605A (en) * | 2013-06-17 | 2013-09-11 | 何寒 | Preparation method of tissue culture medium without agar |
| CN104871980A (en) * | 2015-06-26 | 2015-09-02 | 黄旭胜 | Culture medium for tissue culture |
| CN107328623A (en) * | 2017-07-10 | 2017-11-07 | 成都理工大学 | A kind of cementing transparent soil and preparation method thereof |
| CN107442086A (en) * | 2017-08-25 | 2017-12-08 | 陕西科技大学 | A kind of sorbing material that shape memory function is caused with water and its preparation method and application |
| CN207869971U (en) * | 2017-06-26 | 2018-09-18 | 潍坊市烟草专卖局 | A kind of breeding apparatus of observation tobacco plant root growth |
| KR20180128198A (en) * | 2017-05-23 | 2018-12-03 | 숭실대학교산학협력단 | Customized transparent soil to controllable mechanical properties and its manufacturing method |
-
2018
- 2018-12-04 CN CN201811473457.9A patent/CN109673587B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283605A (en) * | 2013-06-17 | 2013-09-11 | 何寒 | Preparation method of tissue culture medium without agar |
| CN104871980A (en) * | 2015-06-26 | 2015-09-02 | 黄旭胜 | Culture medium for tissue culture |
| KR20180128198A (en) * | 2017-05-23 | 2018-12-03 | 숭실대학교산학협력단 | Customized transparent soil to controllable mechanical properties and its manufacturing method |
| CN207869971U (en) * | 2017-06-26 | 2018-09-18 | 潍坊市烟草专卖局 | A kind of breeding apparatus of observation tobacco plant root growth |
| CN107328623A (en) * | 2017-07-10 | 2017-11-07 | 成都理工大学 | A kind of cementing transparent soil and preparation method thereof |
| CN107442086A (en) * | 2017-08-25 | 2017-12-08 | 陕西科技大学 | A kind of sorbing material that shape memory function is caused with water and its preparation method and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109673587B (en) | 2020-07-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106472228B (en) | Planting method of selenium-rich camellia oleifera | |
| CN104255420B (en) | A kind of seedling medium of melon diseases prevention growth promoting and preparation method thereof | |
| CN107022241B (en) | Nano-silver and gelatin modified antibacterial ink and preparation method thereof | |
| CN105130678A (en) | Organic-inorganic compound fertilizer for reducing salinity and alkalinity and improving soil | |
| CN111410584A (en) | Microbial slow-release compound fertilizer and preparation method thereof | |
| CN107935783A (en) | A kind of microelement type water-soluble fertilizer containing amino-acid liquid and its production technology | |
| CN106748445A (en) | A kind of covering soil used for portabella | |
| WO2021006695A1 (en) | Method for preparing mineral microorganisms | |
| Verma et al. | Decomposition of algal cells and components and their stabilization through complexing with model humic acid-type phenolic polymers | |
| KR101481767B1 (en) | Microorganism agent for soil improvement using condensed molasses solubles | |
| CN109673587A (en) | A kind of preparation method for the transparent earth materials can be used for soil animal movable observing | |
| KR100976445B1 (en) | Physiologically active substance using micro organisms for trees and method for using thereof | |
| CN105876133A (en) | Feed additive for aquatic organisms and preparation method thereof | |
| CN1962563B (en) | Organic fertilizer for aquaculture | |
| CN104774098A (en) | Compound biological bacterium fertilizer and preparation method thereof | |
| CN103710270A (en) | Decomposition agent for degrading litters of pine trees | |
| Walker | Fungus‐alga ultrastructure in the lichen, Cornicularia normoerica | |
| CN113383790A (en) | Environment-friendly potassium hydrogen persulfate compound disinfectant and production process thereof | |
| CN105917927A (en) | Selenium-rich cultivation method for aloes | |
| EP0101784A1 (en) | Process for the preparation of natural humic mould and fertilizers | |
| Adebayo et al. | Effects of Trichoderma longibrachiatum (NGJ167) and compost on early growth of Bougainvillea spectabilis | |
| Li et al. | Hormogonia mass differentiation from Nostoc sphaeroides Kütz.(cyanobacterium) and the comparison of structural characteristics between hormogonia and vegetative filaments | |
| CN1775296A (en) | Composite plant deodorant for livestock and poultry raising | |
| CN1312080C (en) | Method for producing organic fertilizer for flowers by using dejecta of milk cow | |
| CN110384111A (en) | A kind of synergist of lactic acid bacteria and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |