CN109673587B - Preparation method of transparent soil material for soil animal activity observation - Google Patents
Preparation method of transparent soil material for soil animal activity observation Download PDFInfo
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- CN109673587B CN109673587B CN201811473457.9A CN201811473457A CN109673587B CN 109673587 B CN109673587 B CN 109673587B CN 201811473457 A CN201811473457 A CN 201811473457A CN 109673587 B CN109673587 B CN 109673587B
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- Prior art keywords
- fish meal
- soil
- culture medium
- soil material
- transparent soil
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- 239000002689 soil Substances 0.000 title claims abstract description 56
- 241001465754 Metazoa Species 0.000 title claims abstract description 25
- 239000000463 material Substances 0.000 title claims abstract description 23
- 230000000694 effects Effects 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title abstract description 4
- 239000001963 growth medium Substances 0.000 claims abstract description 35
- 235000019733 Fish meal Nutrition 0.000 claims abstract description 33
- 239000001888 Peptone Substances 0.000 claims abstract description 33
- 108010080698 Peptones Proteins 0.000 claims abstract description 33
- 239000004467 fishmeal Substances 0.000 claims abstract description 33
- 235000019319 peptone Nutrition 0.000 claims abstract description 33
- 239000002245 particle Substances 0.000 claims abstract description 19
- 229920002472 Starch Polymers 0.000 claims abstract description 18
- 235000019698 starch Nutrition 0.000 claims abstract description 18
- 239000008107 starch Substances 0.000 claims abstract description 18
- 239000004925 Acrylic resin Substances 0.000 claims abstract description 8
- 229920000178 Acrylic resin Polymers 0.000 claims abstract description 8
- 230000004048 modification Effects 0.000 claims abstract description 6
- 238000012986 modification Methods 0.000 claims abstract description 6
- 229920000881 Modified starch Polymers 0.000 claims abstract description 5
- 239000004368 Modified starch Substances 0.000 claims abstract description 5
- 235000019426 modified starch Nutrition 0.000 claims abstract description 5
- 230000008961 swelling Effects 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000008367 deionised water Substances 0.000 claims description 7
- 229910021641 deionized water Inorganic materials 0.000 claims description 7
- 239000011259 mixed solution Substances 0.000 claims description 4
- 239000006870 ms-medium Substances 0.000 claims 4
- 238000010298 pulverizing process Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 11
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 15
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 15
- 241001233061 earthworms Species 0.000 description 13
- 238000005303 weighing Methods 0.000 description 8
- 239000011347 resin Substances 0.000 description 6
- 229920005989 resin Polymers 0.000 description 6
- 230000007774 longterm Effects 0.000 description 5
- 230000001954 sterilising effect Effects 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 230000006399 behavior Effects 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 239000003292 glue Substances 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 229910000365 copper sulfate Inorganic materials 0.000 description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 1
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000004359 castor oil Substances 0.000 description 1
- 235000019438 castor oil Nutrition 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000002591 computed tomography Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000019837 monoammonium phosphate Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229920006122 polyamide resin Polymers 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- JXKPEJDQGNYQSM-UHFFFAOYSA-M sodium propionate Chemical compound [Na+].CCC([O-])=O JXKPEJDQGNYQSM-UHFFFAOYSA-M 0.000 description 1
- 239000004324 sodium propionate Substances 0.000 description 1
- 235000010334 sodium propionate Nutrition 0.000 description 1
- 229960003212 sodium propionate Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Environmental Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Birds (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Insects & Arthropods (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of a transparent soil material for observing the activity of soil animals; the transparent soil material is formed by adding modified starch grafted acrylic resin into an Ms culture medium containing fish meal peptone to swell; the modification is to crush the starch grafted acrylic resin to a particle size of 0.5-1 mm. The invention can prepare the transparent soil material which can be used for observing the activities of soil animals for a long time by a cheap and rapid method.
Description
Technical Field
The invention belongs to the technical field of agriculture, and particularly relates to a preparation method of a transparent soil material, wherein the prepared material can be used for observing soil animal behaviors.
Background
Soil is a link for communicating water with the atmosphere and is one of the important components in the ecosystem. The soil lives with a plurality of animals, plants and microorganisms, the organisms and the soil are mutually influenced, and the soil quality can be rapidly judged according to the living state of the organisms. Previous activities on soil animals generally required destructive sampling and soil splitting to be seen. The method is also realized by certain technical means, such as CT scanning; however, the method is complex and has high cost, which is not beneficial to popularization and application. Through retrieval, a patent that semi-transparent glue is used for plant culture is found [ application No. 201611000726.0 ], and the patent self states that the invention discloses the semi-transparent plant culture glue which is prepared from the following components in parts by weight: polyamide resin, boric acid powder, sodium nitrate, magnesium sulfate, ammonium sulfate, castor oil, copper sulfate, ammonium dihydrogen phosphate, sodium propionate and a proper amount of water', and the whole process of slow germination of plants can be visually observed. However, the composition of the patent is complex, and soil animals such as earthworms have short survival time in the soil, and the long-term observation cannot be carried out through experiments. The reason may be that the carbon-nitrogen ratio or certain components of the culture medium are not suitable for the long-term survival of soil animals. And the glue prepared by the method contains substances with colors, such as copper sulfate and the like, has low transparency, and has poor observation effect on the moving process of soil animals.
Disclosure of Invention
The present invention is directed to solving the problems set forth in the background art described above, and provides a method for preparing a transparent soil material capable of directly observing the behavior changes of soil animals; the invention can prepare the transparent soil material by a cheap and rapid method, and is simple, convenient and easy to operate.
The purpose of the invention is realized by the following technical scheme:
the invention relates to a transparent soil material, which is formed by adding modified starch grafted acrylic resin into an Ms culture medium containing fish meal peptone to swell.
Preferably, the modification is that the starch grafted acrylic resin is crushed to the particle size of 0.5-1 mm. If the diameter is less than 0.5mm, the diameter of the particles is too small after later swelling, the porosity is reduced, and the animals are not easy to survive; the particle diameter is larger than 1mm, and after later swelling, the particle diameter is too large, and the material shows that various optical refraction, reflection and the like can be formed, so that the transparency is reduced, and the observation is influenced.
Preferably, the Ms culture medium containing the fish meal peptone is obtained by adding the fish meal peptone and the Ms culture medium into deionized water, wherein the content of the fish meal peptone is 25-35 g/L, and the content of the Ms culture medium is 3-5 g/L, and sterilizing the mixture at 121 ℃ for 20-30 min, when the content of the fish meal peptone is lower than 25 g/L and higher than 35 g/L, the whole carbon and nitrogen cannot be in a range suitable for the survival of soil animals, so that the long-term life of the animals is influenced, when the content of the Ms culture medium is lower than 3 g/L, snow-shaped resin swelling is shown to be generated, the observation is not facilitated, and when the content of the culture medium is higher than 5 g/L, the porosity is smaller after the resin swelling, the dissolved oxygen is insufficient, and the long-term survival of the soil animals is not facilitated.
Preferably, the mass-volume ratio of the modified starch grafted acrylic resin to the Ms culture medium containing fish meal peptone is 1: 30-1: 45g/m L, when the mass-volume ratio is less than 1:30, the swelling of the material is insufficient, which indicates that partial swelling of the material is incomplete, and the survival of animals is influenced, and when the mass-volume ratio is more than 1:45, partial swelling of the material has fluidity, and the observation of the activity of the animals is not facilitated.
Preferably, the transparent soil material is used for observing soil animal activities for a long time.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention takes cheap starch grafted acrylic acid, fish meal peptone and Ms culture medium as raw materials, and can be used for observing the behavior of soil animals after modification.
2. The invention takes starch grafted acrylic acid, fish meal peptone and Ms culture medium as raw materials, has low toxicity to soil animals, and can be used for long-term observation of soil animal behaviors.
3. The material of the invention is simple and rapid to prepare, economic and applicable.
Detailed Description
The present invention will be described in detail with reference to examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that it would be apparent to those skilled in the art that several modifications and improvements can be made without departing from the inventive concept. All falling within the scope of the present invention.
Example 1
Weighing 10g of dry starch grafted acrylic acid particles, crushing in a crusher until the particle size reaches 1mm, uniformly mixing for later use, adding fish meal peptone and an Ms culture medium into deionized water, wherein the content of the fish meal peptone is 30 g/L, the content of the Ms culture medium is 4 g/L, sterilizing the mixed solution at 121 ℃ for 30min to obtain an Ms culture medium containing the fish meal peptone, weighing the corresponding mass of the starch grafted acrylic acid particles into the corresponding volume of the Ms culture medium containing the fish meal peptone according to the mass-to-volume ratio of the starch grafted acrylic acid to the sterilized Ms culture medium containing the fish meal peptone of 1: 35, completely swelling resin to obtain transparent soil, placing soil animals such as earthworms into the transparent soil, enabling the activity process of the earthworms in the soil to be seen by naked eyes, stopping the test until two months later, and enabling the earthworms to normally live in the transparent soil.
Example 2
Weighing 10g of dry starch grafted acrylic acid particles, crushing in a crusher until the particle size reaches 0.5mm, uniformly mixing for later use, adding fish meal peptone and an Ms culture medium into deionized water, wherein the content of the fish meal peptone is 25 g/L, the content of the Ms culture medium is 3 g/L, sterilizing the mixed solution at 121 ℃ for 20min to obtain the Ms culture medium containing the fish meal peptone, weighing the starch grafted acrylic acid particles with corresponding mass into the Ms culture medium containing the fish meal peptone according to the mass-volume ratio of the starch grafted acrylic acid to the sterilized Ms culture medium containing the fish meal peptone being 1:30, and putting the earthworms in the Ms culture medium containing the fish meal peptone, and obtaining transparent soil after resin is completely swelled.
Example 3
Weighing 10g of dry starch grafted acrylic acid particles, crushing in a crusher until the particle size reaches 0.8mm, uniformly mixing for later use, adding fish meal peptone and an Ms culture medium into deionized water, wherein the content of the fish meal peptone is 35 g/L, the content of the Ms culture medium is 5 g/L, sterilizing the mixed solution at 121 ℃ for 25min to obtain the Ms culture medium containing the fish meal peptone, weighing the starch grafted acrylic acid particles with corresponding mass into the Ms culture medium containing the fish meal peptone according to the mass-volume ratio of the starch grafted acrylic acid to the sterilized Ms culture medium containing the fish meal peptone being 1:45, completely swelling resin to obtain transparent soil, placing soil animals such as earthworms into the transparent soil, enabling the activity process of the earthworms in the soil to be seen visually, stopping the test after two months, and enabling the earthworms to normally live in the transparent soil.
Comparative example 1
Weighing 10g of dry starch grafted acrylic acid particles, crushing in a crusher until the particle size reaches 1mm, uniformly mixing for later use, adding an Ms culture medium into deionized water, wherein the content of the Ms culture medium is 4 g/L, sterilizing at 121 ℃ for 30min to obtain a sterilized Ms culture medium, weighing starch grafted acrylic acid particles with corresponding mass according to the mass-to-volume ratio of the starch grafted acrylic acid to the sterilized Ms culture medium of 1: 35, putting the starch grafted acrylic acid particles with corresponding mass into the sterilized Ms culture medium with corresponding volume, and obtaining transparent soil after resin is completely swelled.
Comparative example 2
This comparative example is substantially the same as example 1 except that: ammonium sulfate was used instead of fish meal peptone to provide a nitrogen source. When soil animals such as earthworms are placed in the transparent soil of the comparative example, the moving process of the earthworms in the soil can be seen by naked eyes, but after half a month, the moving capability of the earthworms is weakened, the girdle of the earthworms is partially swollen, and part of the links are curled. Indicating that the earthworms can not live in the transparent soil for a long time.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes and modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention.
Claims (5)
1. The transparent soil material is characterized in that the transparent soil material is formed by adding modified starch grafted acrylic resin into an Ms culture medium containing fish meal peptone and swelling; the transparent soil material is used for observing soil animal activities for a long time.
2. The transparent soil material according to claim 1, wherein the modification is a pulverization of the starch graft acrylic resin to a particle size of 0.5 to 1 mm.
3. The transparent soil material of claim 1, wherein the Ms medium containing fish meal peptone is prepared by adding fish meal peptone and Ms medium into deionized water, wherein the content of the fish meal peptone is 25-35 g/L, and the content of the Ms medium is 3-5 g/L.
4. The transparent soil material as claimed in claim 3, wherein the mixed solution of fish meal peptone and Ms culture medium added with deionized water is sterilized at 121 ℃ for 20-30 min to obtain the Ms culture medium containing fish meal peptone.
5. The transparent soil material as claimed in claim 1, wherein the mass volume ratio of the modified starch grafted acrylic resin to the Ms medium containing fish meal peptone is 1: 30-1: 45g/m L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811473457.9A CN109673587B (en) | 2018-12-04 | 2018-12-04 | Preparation method of transparent soil material for soil animal activity observation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811473457.9A CN109673587B (en) | 2018-12-04 | 2018-12-04 | Preparation method of transparent soil material for soil animal activity observation |
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| Publication Number | Publication Date |
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| CN109673587A CN109673587A (en) | 2019-04-26 |
| CN109673587B true CN109673587B (en) | 2020-07-24 |
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|---|---|---|---|---|
| CN103283605A (en) * | 2013-06-17 | 2013-09-11 | 何寒 | Preparation method of tissue culture medium without agar |
| CN104871980A (en) * | 2015-06-26 | 2015-09-02 | 黄旭胜 | Culture medium for tissue culture |
| CN107328623A (en) * | 2017-07-10 | 2017-11-07 | 成都理工大学 | A kind of cementing transparent soil and preparation method thereof |
| CN107442086A (en) * | 2017-08-25 | 2017-12-08 | 陕西科技大学 | A kind of sorbing material that shape memory function is caused with water and its preparation method and application |
| CN207869971U (en) * | 2017-06-26 | 2018-09-18 | 潍坊市烟草专卖局 | A kind of breeding apparatus of observation tobacco plant root growth |
| KR20180128198A (en) * | 2017-05-23 | 2018-12-03 | 숭실대학교산학협력단 | Customized transparent soil to controllable mechanical properties and its manufacturing method |
-
2018
- 2018-12-04 CN CN201811473457.9A patent/CN109673587B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103283605A (en) * | 2013-06-17 | 2013-09-11 | 何寒 | Preparation method of tissue culture medium without agar |
| CN104871980A (en) * | 2015-06-26 | 2015-09-02 | 黄旭胜 | Culture medium for tissue culture |
| KR20180128198A (en) * | 2017-05-23 | 2018-12-03 | 숭실대학교산학협력단 | Customized transparent soil to controllable mechanical properties and its manufacturing method |
| CN207869971U (en) * | 2017-06-26 | 2018-09-18 | 潍坊市烟草专卖局 | A kind of breeding apparatus of observation tobacco plant root growth |
| CN107328623A (en) * | 2017-07-10 | 2017-11-07 | 成都理工大学 | A kind of cementing transparent soil and preparation method thereof |
| CN107442086A (en) * | 2017-08-25 | 2017-12-08 | 陕西科技大学 | A kind of sorbing material that shape memory function is caused with water and its preparation method and application |
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| CN109673587A (en) | 2019-04-26 |
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