CN109652084A - A kind of petroleum hydrocarbon contaminated soil microorganism coupling degradation agent and its application method - Google Patents
A kind of petroleum hydrocarbon contaminated soil microorganism coupling degradation agent and its application method Download PDFInfo
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- CN109652084A CN109652084A CN201910038047.XA CN201910038047A CN109652084A CN 109652084 A CN109652084 A CN 109652084A CN 201910038047 A CN201910038047 A CN 201910038047A CN 109652084 A CN109652084 A CN 109652084A
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- China
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- soil
- petroleum hydrocarbon
- application method
- contaminated soil
- degradation agent
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Processing Of Solid Wastes (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The present invention relates to a kind of petroleum hydrocarbon contaminated soils to repair microorganism coupling degradation medicament and application method, the degrading microorganism are as follows: acinetobacter calcoaceticus, degradation medicament is made of ten disulfobenzene sodium sulfonates, fatty alcohol polyoxyethylene ether, isomerous tridecanol polyoxyethylene ether, mass percent: 2.8%, 2.2%, 5.0%.The present invention first carries out soil pretreatment, and Tri-color is mixed with soil, promotes the emulsification and dissolution of petroleum.High-effective microorganism is added again, expands the contact area of microorganism and petroleum, to improve the degradation efficiency of petroleum hydrocarbon.The Tri-color degradation agent is nontoxic, is matched by the compound of non-toxic herb and the special of surfactant.The degradation rate of petroleum hydrocarbon can be reached 74% at 3 months by the technology, and reparation more independent than high-effective microorganism improves 18%.The removal rate of soil petroleum hydrocarbon is substantially increased using the technology.
Description
Technical field
The invention belongs to environment remediation technical fields, are related to Tri-color degradation agent and combine reparation soil with microbial bacterial agent
The method of earth is related to a kind of petroleum hydrocarbon contaminated soil and repairs microorganism coupling degradation agent (TRI-COLORI) and application method.
Background technique
Petroleum is the main energy sources of the world today, and the demand of countries on petroleum is also sharply increasing, in oil exploitation, fortune
Defeated, processing, in use process there are different degrees of leakage discharge, caused by pollution problem be also on the rise.In China
Investigation of Soil Pollution in 2015 reports display, and the soil that at least 5,000,000 hectares of China is dirty by different degrees of petroleum hydrocarbon
Dye, petroleum hydrocarbon enter after soil by plants enriched in vivo, eventually enter into human body by food chain, endanger human health.
Reparation for soil petroleum hydrocarbon contaminated soil is the main problem faced at present, and bioremediation technology refers to
Some nutriments and high efficient strain are added in pollution environment, it is mesh that making contaminant degradation or passivation, which is nontoxic product,
A kind of preceding most promising environment restoring method.
Petroleum hydrocarbon is a kind of mixture, has hydrophobicity, and surfactant can reduce the boundary of oil substance and aqueous solution
Face tension increases the dissolubility of petroleum, contacts microorganism and petroleum more effectively, to accelerate the degradation of petroleum hydrocarbon.
Summary of the invention
It is of the invention the technical problem to be solved is that: the degradation rate of microorganism remediation petroleum hydrocarbon is low, and degradation efficiency is slow, mentions
Medicament is repaired for a kind of novel practical, the efficient petroleum hydrocarbon contaminated soil to solve the above problems.
The technical principle that the present invention uses is: degradation agent reduces the interface tension between petroleum substance and aqueous solution, increases
The dissolubility of petroleum contacts petroleum and microorganism preferably, to accelerate the biodegrade of petroleum.Include the following steps:
By the broken sieving of petroleum hydrocarbon contaminated soil in experimentation, it is put into sizeable small flower, natural air drying；
It will be diluted in medicament in water with 1:49, then dilute medicament and be added in small flower, be uniformly mixed with soil, make soil
It is 0.1% containing pharmaceutical quantities；
The activation of efficient degrading bacteria acinetobacter calcoaceticus is inoculated into 100mlLB culture medium, LB culture medium composition: peptone 10g/L, ferment
Female powder 5g/L, sodium chloride 10g/L, natural pH, 121 DEG C of high pressure steam sterilization 20min are spare.30 DEG C in LB culture medium,
150rpm is cultivated 3 days, and bacteria containing amount is made to reach 109Bacterium solution is made in a/ml or more.
Bacterium solution and water are mixed with 1:9 ratio, pouring into contaminated soil makes bacteria containing amount reach 107A/g；
Continue to keep the skin wet to contaminated soil, make soil moisture content 25-30%, is placed in ventilation culture, keeps the skin wet in due course,
Save ground moistening.
Beneficial effect
1. microorganism is combined with Tri-color degradation agent plays synergistic effect, Tri-color is mixed with soil, promotes petroleum
Emulsification and dissolution.It contacts microorganism and petroleum more effectively, promotes microorganism preferably to grow, to improve petroleum hydrocarbon
Degradation efficiency；
2. the present invention used degradation agent, there is fine petroleum dispersibility, increases the table specific area of petroleum, improve micro- life
The contact area of object, and be easy to transport and use, safety is more preferable, does not need the pH of regulation system；
3.Tri-color degradation agent-high-effective microorganism joint repairs and can reach 74% in the degradation rate of 3 months petroleum hydrocarbons,
Reparation more independent than high-effective microorganism improves 18%.The degradation rate of soil petroleum hydrocarbon is substantially increased using the technology；
Detailed description of the invention
The microorganism of Fig. 1 embodiment 1 adds the degradation rate of Tri-color degradation bacteria reparation petroleum hydrocarbon contaminated soil.
Specific embodiment
Embodiment 1: Hangzhou Microbiological Lab, Zao Pin Science and Technology Ltd. carries out potted plant experiment
It is ground after being air-dried to the contaminated soil for picking up from Panjin Shuguang Oilfield, crosses 2.5mm sieve.Soil oil content is measured by sampling,.
One soil oil content of table and pH Indexs measure
| Testing index | Oil content | pH |
| Contaminated soil sample | 0.45% | 7.8 |
Table 1
It takes 200g soil to be put into small flower, takes 10ml through 50 times of diluted medicaments, stir evenly.
2ml bacterium solution is poured into flowerpot, being uniformly mixed makes soil bacteria containing amount be greater than 107A/g, keeping the skin wet makes soil
Earth water content reaches 25-30%, is placed in ventilation aerlbic culture 3 months, and keep the skin wet moisturizing in due course.
The degradation effect for being individually added into microbial inoculum and addition microbial inoculum and Tri-color degradation agent is shown in Fig. 1.By in figure it is found that single
The processing degradation rate that microbial bacterial agent solely is added only has 56%, and the processing of microbial bacterial agent joint Tri-color degradation agent is added
Degradation rate reaches 74% within 3 months, improves 18% than the processing of independent microbial bacterial agent, significant effect.
Claims (11)
1. a kind of petroleum hydrocarbon contaminated soil repairs microorganism and couples degradation agent, the medicament is by ten disulfobenzene sodium sulfonates, fatty alcohol
Polyoxyethylene ether, isomerous tridecanol polyoxyethylene ether and water composition, the mass percent of each component are ten disulfobenzene sodium sulfonates
2.5-3%, fatty alcohol polyoxyethylene ether 1.8-2.4%, isomerous tridecanol polyoxyethylene ether 4.8-5.2%, surplus are water.
2. a kind of petroleum hydrocarbon contaminated soil according to claim 1 repairs microorganism and couples degradation agent, which is characterized in that
The mass percent of ten disulfobenzene sodium sulfonates is 2.8%, fatty alcohol polyoxyethylene ether 2.2%, isomerous tridecanol polyoxyethylene
Ether 5%.
3. degradation agent described in claim 1 reparation petroleum hydrocarbon contaminated soil application method, which is characterized in that be added degradation agent and
The improvement of microbial bacterial agent progress soil.
4. application method according to claim 3, which is characterized in that the microbial bacterial agent is acinetobacter calcoaceticus.
5. application method according to claim 3, which is characterized in that microbial bacterial agent is one plant of acinetobacter calcoaceticus, passes through kind
Sub- culture medium activation, then be transferred to fermentation in LB culture medium and expand culture, so that bacteria containing amount is reached 109A/ml.
6. a kind of petroleum hydrocarbon contaminated soil according to claim 1 repairs microorganism and couples degradation agent, which is characterized in that
The amount of the degradation agent is the 0.1% of petroleum hydrocarbon contaminated soil quality.
7. application method according to claim 2, which is characterized in that mix degradation agent and water, uniformly sprinkling and soil
Earth mixing, chemical content 0.1%.
8. application method according to claim 6, which is characterized in that mix degradation agent and water 1:49 in mass ratio
Close dilution.
9. application method according to claim 3, which is characterized in that be uniformly mixed microbial inoculum with water and be sprayed onto contaminated soil
In, the use of bacterium amount is that 1ml bacterium solution matches 100g soil, bacteria containing amount in soil is made to be greater than 107A/g, the final water content of soil are
25-30%.
10. application method according to claim 9, which is characterized in that bacterium solution and water are with the mixing of volume 1:9 ratio.
11. application method according to claim 2, which is characterized in that degradation agent and microbial bacterial agent is added in contaminated soil
Afterwards, continue to keep the skin wet to contaminated soil, make soil moisture content 25-30%, be placed in ventilation culture, keep the skin wet in due course,
Save ground moistening.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910038047.XA CN109652084A (en) | 2019-01-16 | 2019-01-16 | A kind of petroleum hydrocarbon contaminated soil microorganism coupling degradation agent and its application method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910038047.XA CN109652084A (en) | 2019-01-16 | 2019-01-16 | A kind of petroleum hydrocarbon contaminated soil microorganism coupling degradation agent and its application method |
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| CN109652084A true CN109652084A (en) | 2019-04-19 |
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| CN201910038047.XA Pending CN109652084A (en) | 2019-01-16 | 2019-01-16 | A kind of petroleum hydrocarbon contaminated soil microorganism coupling degradation agent and its application method |
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Citations (7)
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| CN105733976A (en) * | 2015-12-29 | 2016-07-06 | 中国科学院烟台海岸带研究所 | Composite inoculant for degrading petroleum, preparation method and applications thereof |
| CN106283075A (en) * | 2016-08-03 | 2017-01-04 | 上海赛敏环保科技有限公司 | Water-solube and biodegradable abluent and Cleaning application method |
| CN107974357A (en) * | 2017-10-31 | 2018-05-01 | 建德市环保科技创新创业中心有限公司 | A kind of heavy oil dirt biological cleaner and preparation method thereof |
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2019
- 2019-01-16 CN CN201910038047.XA patent/CN109652084A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060193700A1 (en) * | 2005-02-25 | 2006-08-31 | Putnam John M | Method of using anionic copolymer formulations for seepage control |
| CN103058474A (en) * | 2012-07-10 | 2013-04-24 | 郑涵 | Bioremediation type oil-contained silt separating agent and preparation method thereof |
| CN103695332A (en) * | 2013-10-18 | 2014-04-02 | 环境保护部华南环境科学研究所 | Novel diesel oil pollution type place microbe degrading bacteria |
| CN105040014A (en) * | 2015-08-06 | 2015-11-11 | 抚顺克立恩清洗有限公司 | Environment-friendly efficient fouling cleaning agent |
| CN105733976A (en) * | 2015-12-29 | 2016-07-06 | 中国科学院烟台海岸带研究所 | Composite inoculant for degrading petroleum, preparation method and applications thereof |
| CN106283075A (en) * | 2016-08-03 | 2017-01-04 | 上海赛敏环保科技有限公司 | Water-solube and biodegradable abluent and Cleaning application method |
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Application publication date: 20190419 |