CN109645012A - A kind of medicament and its preparation method and application for preventing and treating ring rot of apple - Google Patents
A kind of medicament and its preparation method and application for preventing and treating ring rot of apple Download PDFInfo
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- CN109645012A CN109645012A CN201811652753.5A CN201811652753A CN109645012A CN 109645012 A CN109645012 A CN 109645012A CN 201811652753 A CN201811652753 A CN 201811652753A CN 109645012 A CN109645012 A CN 109645012A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/50—1,3-Diazoles; Hydrogenated 1,3-diazoles
- A01N43/52—1,3-Diazoles; Hydrogenated 1,3-diazoles condensed with carbocyclic rings, e.g. benzimidazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/24—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients to enhance the sticking of the active ingredients
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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Abstract
The invention discloses a kind of medicaments and its preparation method and application for preventing and treating ring rot of apple, belong to fungicide technical field, the medicament includes gel skeleton and modification of chitosan, the modification of chitosan is formed after chitosan graft glyoxaline compound by formic acid modification, the gel skeleton raw material includes for guar gum, Cellulose Acrylate, carbopol, the glyoxaline compound is 2- chloromethyl benzimidazole, medicament is added water and stirred into gel, it is then applied at the disease for having struck off disease tumor and uses, medicament disclosed by the invention is exclusively used in preventing ring rot of apple, and good drug efficacy, efficacy time is long, drug resistance generates slow.
Description
Technical field
The present invention relates to fungicide technical field more particularly to a kind of medicaments and preparation method thereof for preventing and treating ring rot of apple
And application.
Background technique
The ring spot of apple includes two kinds of hazard symptoms of Trunk canker and fruit ring spot.Trunk canker is exactly taken turns
After line germ invades the hole skin of apple branch, the small knob of protrusion is formed on hole skin, second year starts to expand, and general the
Disease tumor is reformed within 3 years, typical knob diameter, which is 0.3-3 centimetres, to be differed, and slight crack is just generated around the sick tumor on 3 or four years branches
With stick up skin.If encountering good growing weather simultaneously, at central protrusion around there is scattered black small grain point (pycnidia), just
Germ spore is distributed to start to infect again.When pomegranate shape scab on trunk and major branch occurs serious, sick portion's bark is coarse, is in rough bark
Shape, therefore have the title of scurf.Germ pycnidia in overwintering limb tumor skin scab has and constantly produces sporogenic ability,
It is the main source for infecting fruit germ.So the disease gently then encroaches on Apple, limb once infecting, weakens tree vigo(u)r, draw
Playing yield and nutrient quality reduces, and serious person can lead to the underproduction and even damage entire apple orchard.And China's ring rot of apple
Overall incidence is up to 70% or more, especially aggrieved the most serious with Fuji and Gold Delicious apple, and it is left generally to can lead to the underproduction 30%
The right side, in high temperature and rainy season, decayed fruit rate is very serious up to up to 40-60%, harmfulness.
Ring spot germ is overwintering in killed limb with mycelia, pycnidia and the shell of ascus.Mycelia is in limb diseased tissues
It can survive 4~5 years, generate spore between annual 4~June, become primary infection source.7~August spore distributes more；Before sick portion
The three sporogenic abilities of annual output are strong, gradually weaken later.Conidium is splashed depending mainly on rainwater propagates, and range is usually no more than 10
Rice.Germ can infect young fruit in 10 days or so after fallen flowers.4~July, the amount of infecting was most, and the scattered amount of spore reduces after July,
Fruit is also not easy to be infected simultaneously, and infection rate is very low.Spore germination percutaneous (fruit dot) intrusion fruit and limb, 24 hours achievable
It infects.
The upper chemical bactericide that mainly passes through of production at present prevents and treats ring spot, Mancozeb, carbendazim, thiophanate methyl, first
The fungicide such as base thiophanate, difoltan are the common medicaments for preventing and treating ring spot, these medical treatment rings rot of apple are used for a long time,
The problem of will appear drug resistance and sensibility decline, and drug is easy residual, remaining pesticide can be detrimental to health.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of medicament for preventing and treating ring rot of apple and preparation method thereof and answering
With the medicament of preparation is exclusively used in preventing ring rot of apple, and good drug efficacy, and efficacy time is long, and drug resistance generates slow.
The present invention solves above-mentioned technical problem by following technological means:
A kind of medicament for preventing and treating ring rot of apple, the medicament includes gel skeleton and modification of chitosan, the modified shell
Glycan is formed after chitosan graft glyoxaline compound by formic acid modification, the gel skeleton raw material include for guar gum,
Cellulose Acrylate, carbopol.
Further, the molecular weight of the chitosan is 6000-9000, deacetylation 80-90%.
Further, the glyoxaline compound is 2- chloromethyl benzimidazole, the chitosan and 2- chloromethyl benzo miaow
The mass ratio of azoles is 1:(0.2-0.5).
Further, the concentration of the formic acid is 45wt%.
The invention also discloses a kind of preparation methods of medicament for preventing and treating ring rot of apple:
Colloidal solution preparation: after 30-50 parts by weight guar gum is dissolved in the ethanol solution of 35wt%, 30-40 weight is added
Part Cellulose Acrylate, 10-15 parts by weight mannitol, 10-13 parts by weight magnesium stearate, heating water bath adjust body to 50-60 DEG C
It is pH=7, after stirring 1-2h, heating water bath obtains colloidal solution to 65-75 DEG C；
Gel preparation: colloidal solution, 15-25 parts by weight are uniformly mixed without ammonia natural emulsion, after being heated to 70-80 DEG C,
10-15 weight account polyethylene, 1-3 parts by weight of crosslinking agent is added, 30-40min is stirred with the speed of 200-300rpm, is then added
50-70 parts by weight guar gum, 40-60 parts by weight carbopol, regulation system pH=6 obtain gel after stirring 1-2h；
Medicament preparation: being ventilated with the air velocity of 25L/min to gel, while with the stirring of the rate of 2000-3000rpm
The part by weight modified chitosan of 40-80 is added after 20-30min, is taken out after stirring 2-3h, in 60-70 DEG C of microwave drying, crushing is obtained
Medicament.
Further, the preparation step of the modification of chitosan is as follows:
Chitosan is added in 2% acetic acid solution, is configured to the chitosan solution of 2-3wt%, 2- chloromethylbenzene is added
And imidazoles, 80 DEG C, under nitrogen atmosphere are heated with stirring to, the mixed solution of nitric acid and hydrochloric acid is instilled, is cooled to 50 DEG C, nitric acid is added
Cerium ammonium after being stirred to react 10-12h, is added formic acid, ethylene glycol, ethyl alcohol, deionized water, is heated to 70-80 DEG C, and flow back 18-24h
After be cooled to room temperature, after suction filtration clean, powdered modification of chitosan is dried to obtain at 40 DEG C.
Further, in the preparation step of the modification of chitosan, mixed solution is the nitric acid and 3wt% hydrochloric acid by 2wt%
It is mixed according to the volume ratio of 1:1.
Further, in the preparation step of the modification of chitosan, the volume ratio of formic acid and ethylene glycol is 1:0.3.
Further, the medicament is mixed with water according to the volume ratio of 1:5, stirs into gel, is then applied to and has struck off
At the disease of sick tumor.
The invention discloses a kind of medicaments and its preparation method and application for preventing and treating ring rot of apple, have the beneficial effect that:
1, gel skeleton has viscosity, can be adhered in tree trunk, can reside at disease for a long time, extends drug effect,
And gel skeleton has slow releasing function, and the mesh that can reach Long term control prevention ring rot of apple is compounded with modification of chitosan
, prevention and treatment efficiency can be improved to 90% or more.
2, use chitosan have natural biocidal property, can also induce plant generate chitosan enzyme, plant protection element,
The substances such as lignin, peroxidase, protect the plants from the infringement of pathogen, and are also used as soil conditioner.
3, after chitosan graft 2- chloromethyl benzimidazole, the bactericidal effect of chitosan is more preferable, sterilizes rapider, drug effect
Time with length, is more suitable for the apple tree with serious ring spot.And because and chitosan graft, form the object of like derivatives
Matter, toxicity reduce, and irritation reduces, safer.
4, there is chitosan more free amino could accelerate to be grafted with 2- chloromethyl benzimidazole, and deacetylated
It spends bigger, is more insoluble in water, in order to improve dissolubility, needs to be modified grafting rear shell glycan, using formic acid by chitosan
Hydroxyl be esterified, improve the dissolubility of modified chitosan, when preventing and treating ring rot of apple, be more soluble in water, make
It is standby at liquid, conducive to being sprayed on illness plant.
Specific embodiment
Below with reference to specific embodiment, the present invention is described in detail:
Embodiment 1: modification of chitosan preparation one:
It is 80% by deacetylation, the 80g chitosan that molecular weight is 6000 is added in the acetic acid solution of 300ml 2%, shell
The deacetylation of glycan is higher, and free amino is more, is less susceptible to dissolve, therefore needs that acetic acid solution is added, and is dissolved in chitosan
In acetic acid solution, configuration forms the chitosan solution of 2wt%, and 16g2- chloromethyl benzimidazole, agitating and heating are added under room temperature
To 80 DEG C, 2- chloromethyl benzimidazole belongs to glyoxaline compound, has strong bactericidal effect, hot water is dissolved in, to 2- chloromethane
After base benzimidazole is completely dissolved, stop heating, under nitrogen atmosphere, instilling 2wt% nitric acid and 3wt% hydrochloric acid according to volume is
The mixed solution 50ml of the ratio preparation of 1:1, is cooled to 50 DEG C, 0.5g ammonium ceric nitrate initiator is added, with the speed of 200r/min
Rate is stirred to react 10h in 50 DEG C, and 2- chloromethyl benzimidazole is as ligand, and under the action of acid condition and initiator, shell is poly-
Sugar amino dehydrogenation after with 2- chloromethyl benzimidazole be grafted, be added 90ml 45wt% formic acid, 27ml ethylene glycol, 50ml ethyl alcohol,
100ml deionized water is heated to 70 DEG C, flows back after 18h, the deacetylation of chitosan is higher, is insoluble in water, will be connect using formic acid
Branch has the chitosan of imidazoles to be modified, and by the hydroxyl and formic acid generation esterification on chitosan, improves dissolubility, stops anti-
Ying Hou is cooled to room temperature, and is washed with deionized water after product is filtered, and is dried to obtain medicament at 40 DEG C.
Chitosan promotes except plant growth, is also used as a kind of natural in addition to can be used as plant growth regulator
Bacteriostatic agent, directly inhibition plant pathogenic microorganisms, and induce plant generate resistance of wide spectrum, enhance the self-defence energy of plant
Power, as natural antimicrobial substance, resistance is good, and will not pollute to environment, but bactericidal effect is slow.Glyoxaline compound is
A kind of common bactericide, but its toxicity is stronger, if having after a large amount of uses residual will cause pollution and harm therefore in order to
The antibiotic property for enhancing medicament, chitosan is modified, chitosan graft imidazoles is made, reaches good anti-bacterial effect, resistance is good, medicine
Imitate fast purpose.And deacetylating degree of chitosan is high, dissolubility is poor, and modified solubility improves, and is particularly suited for apple wheel line
Sick sterilization processing.
Embodiment 2: modification of chitosan preparation two:
It will be 90% by deacetylation, the 60g chitosan that molecular weight is 7000 is added in the acetic acid solution of 250ml 2%,
It is then configured to the chitosan solution of 3wt%, 30g 2- chloromethyl benzimidazole is added under room temperature, is heated with stirring to 80 DEG C,
After 2- chloromethyl benzimidazole is completely dissolved, stop heating, under nitrogen atmosphere, instills 2wt% nitric acid and 3wt% hydrochloric acid is pressed
According to mixed solution 50ml prepared by the ratio that volume is 1:1,50 DEG C are cooled to, 0.3g ammonium ceric nitrate initiator is added, with 200r/
The rate of min is stirred to react 11h in 50 DEG C, and 70ml 45wt% formic acid, 21ml ethylene glycol, 50ml ethyl alcohol, 100ml is then added
Deionized water is heated to 80 DEG C, after reflux for 24 hours, stops reaction, be cooled to room temperature, be washed with deionized water after product is filtered,
Medicament is dried to obtain at 40 DEG C.
Embodiment 3: modification of chitosan preparation three:
It is 85% by deacetylation, the 100g chitosan that molecular weight is 6000 is added in the acetic acid solution of 350ml 2%, with
It is configured to the chitosan solution of 3wt% afterwards, 30g 2- chloromethyl benzimidazole is added under room temperature, is heated with stirring to 80 DEG C, to
After 2- chloromethyl benzimidazole is completely dissolved, stop heating, under nitrogen atmosphere, instill 2wt% nitric acid and 3wt% hydrochloric acid according to
Mixed solution 70ml prepared by the ratio that volume is 1:1, is cooled to 50 DEG C, 0.5g ammonium ceric nitrate initiator is added, with 200r/
The rate of min is stirred to react 11h in 50 DEG C, then be added 100ml 45wt% formic acid, 30ml ethylene glycol, 50ml ethyl alcohol,
100ml deionized water is heated to 75 DEG C, after the 20h that flows back, stops reaction, is cooled to room temperature, uses deionized water after product is filtered
It cleans, is dried to obtain medicament at 40 DEG C.
Embodiment 4: it prevents and treats the medicament preparation of ring rot of apple and applies one:
Colloidal solution preparation: after 30g guar gum is dissolved in the ethanol solution of 300ml 35wt%, 30g acrylic fibers are added
Dimension element, 10g mannitol, 12g magnesium stearate stir evenly, and heating water bath is to 50 DEG C, with the speed of 200rpm after regulation system pH=7
Rate stirs 1h, continues thereafter with heating water bath to 65 DEG C, obtains colloidal solution；
Gel preparation: it by colloidal solution, 15g without ammonia natural emulsion, is uniformly mixed, after being heated to 70 DEG C, the poly- second of 10g is added
Then alkene, 1g crosslinking agent glutaraldehyde stir 35min with the speed of 200rpm, 50g guar gum, 40g carbopol are then added, adjusts
Section system pH=6 obtains gel after stirring 1h；
Medicament preparation: with the air velocity of 25L/min to gel blowing air, while 30min is stirred with the rate of 2000rpm
Afterwards, modification of chitosan prepared by 40g embodiment 1 is added, is taken out after stirring 2h, in 60 DEG C of microwave dryings, obtains medicament after crushing.
The ring spot of apple tree is often sent out in tree trunk, and after sick tumor is struck off, medicament manufactured in the present embodiment is added water and stirred
It at gel, is applied at the disease for striking off sick tumor, prevents and treats ring spot.It, can because there is viscosity after gel skeleton water suction
Persistently anti-ring spot is sticked at disease with viscous, and because gel skeleton has certain slow-release function, it is possible to extend drug effect.
Guar gum is graft-polymerized with Cellulose Acrylate, extends the holding time of medicament, while also extending drug effect.?
High-speed stirred after mixing gel and modification of chitosan under conditions of ventilation, gel inside form many fine and close minute bubbles, change
Property chitosan is attached to inside bubble, and by microwave drying, bubble gradually ruptures, and gel is dehydrated to form gel skeleton.Because changing
Property chitosan is easily separated with gel in whipping process, thus regulation system pH > 5, as pH=6, the carboxylic acid group of carbopol
Degree of ionization is big, takes negative electrical charge, and modification of chitosan is positively charged under mild acid conditions, under stirring conditions, modified
Chitosan attracts each other with gel, and the two is bonded together, not easily to fall off.Added in gel without ammonia natural rubber, poly- second
Alkene belongs to high-molecular organic material, and the chemical stability of gel can be improved and increase the intensity of gel, resistance of rainwater washing against is smeared
After at the Disease of Apple Trees, long action time.
Embodiment 5: it prevents and treats the medicament preparation of ring rot of apple and applies two:
Colloidal solution preparation: after 50g guar gum is dissolved in the ethanol solution of 600ml 35wt%, 40g acrylic fibers are added
Dimension element, 15g mannitol, 13g magnesium stearate stir evenly, and heating water bath is to 60 DEG C, with the speed of 200rpm after regulation system pH=7
Rate stirs 2h, continues thereafter with heating water bath to 75 DEG C, obtains colloidal solution；
Gel preparation: it by colloidal solution, 25g without ammonia natural emulsion, is uniformly mixed, after being heated to 80 DEG C, the poly- second of 15g is added
Then alkene, 3g crosslinking agent glutaraldehyde stir 40min with the speed of 300rpm, 70g guar gum, 60g carbopol are then added, adjusts
Section system pH=6 obtains gel after stirring 2h；
Medicament preparation: with the air velocity of 25L/min to gel blowing air, while 30min is stirred with the rate of 3000rpm
Afterwards, modification of chitosan prepared by 80g embodiment 2 is added, is taken out after stirring 3h, in 70 DEG C of microwave dryings, obtains medicament after crushing.
The ring spot of apple tree is often sent out in tree trunk, and after sick tumor is struck off, medicament manufactured in the present embodiment is added water and stirred
It at gel, is applied at the disease for striking off sick tumor, prevents and treats ring spot.
Ring rot of apple prevention and treatment experiment:
Experimental subjects is apple Fuji apple kind, and the age of tree about 12 years, seeding row spacing was 3 × 4m, high 2.3m or so is set, in tree vigo(u)r
Deng soil is laterite, middle fertility；
Experimental pharmacy be embodiment 4 (A) and embodiment 5 (B) preparation medicament and 50% Baijunjing wettable powder (C),
70% mancozeb wettable powder (D), blank control group (E)；
5 treatment regions of experimental setup, each treatment region are chosen 7 plants of trees and are tested, embodiment 4 and embodiment 5 are obtained
Medicament and after water is prepared into gel according to the volume ratio of 1:5, be applied at the disease for striking off sick tumor, the every place of A medicament is smeared
About 3g is smeared at the every place of about 2g, B medicament, is repeated 2 times；C, D is diluted to 1000 times of liquid, is sprayed at the disease that disease tumor is struck off, liquid medicine
Dosage is 4L/ plants, is repeated 2 times；
After being administered to the rainfall of first time in April for the first time, it is administered to late June for the second time；
Picking apple is carried out in apple picking time, records every treatment region the total number of fruits, the number of sick fruits, in every place's treatment region southeast west
No disease fruit 50 is won in northern four orientation at random, and totally 200,15 days investigation the number of sick fruits are stored under room temperature, is calculated diseased fruit rate and is prevented
Effect is controlled, obtained data are as shown in table 1:
Table 1
Control efficiency=(control group area diseased fruit rate-treatment region diseased fruit rate)/check plot diseased fruit rate × 100%
It can be seen from Table 1 that preventing and treating by the germ in the sick tumor to limb, and then reduce Apple wheel line
The medicament of the generation of disease, embodiment 4 (A) and embodiment 5 (B) preparation is good to ring rot of apple control efficiency, the prevention and treatment of picking time
Effect reaches 100%, and the control efficiency of storage period reaches 90% or more, 50% Baijunjing wettable powder (C), 70% maneb
Zinc wettable powder (D) also has certain control efficiency, but medicament medicine prepared by embodiment 4 and embodiment 5 to ring rot of apple
It imitates significant or extremely significant better than two kinds of medicaments of C, D；The medicament drug effect difference that embodiment 4 and embodiment 5 are prepared respectively is little, drug effect
Quite.Illustrate medicament prepared by the present invention and good drug efficacy, efficacy time is long, and drug resistance generates slow.
The above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to preferred embodiment to this hair
It is bright to be described in detail, those skilled in the art should understand that, it can modify to technical solution of the present invention
Or equivalent replacement should all cover without departing from the objective and range of technical solution of the present invention in claim of the invention
In range.Technology not described in detail in the present invention, shape, construction portion are well-known technique.
Claims (9)
1. a kind of medicament for preventing and treating ring rot of apple, which is characterized in that the medicament includes gel skeleton and modification of chitosan, institute
Stating modification of chitosan is formed after chitosan graft glyoxaline compound by formic acid modification, and the gel skeleton raw material includes being
Guar gum, Cellulose Acrylate, carbopol.
2. a kind of medicament for preventing and treating ring rot of apple according to claim 1, which is characterized in that the molecule of the chitosan
Amount is 6000-9000, deacetylation 80-90%.
3. a kind of medicament for preventing and treating ring rot of apple according to claim 2, which is characterized in that the glyoxaline compound
For 2- chloromethyl benzimidazole, the mass ratio of the chitosan and 2- chloromethyl benzimidazole is 1:(0.2-0.5).
4. a kind of medicament for preventing and treating ring rot of apple according to claim 3, which is characterized in that the concentration of the formic acid is
45wt%.
5. a kind of preparation method of medicament for preventing and treating ring rot of apple according to claim 4, which is characterized in that the medicine
Agent the preparation method is as follows:
Colloidal solution preparation: after 30-50 parts by weight guar gum is dissolved in the ethanol solution of 35wt%, 30-40 parts by weight third are added
Olefin(e) acid cellulose, 10-15 parts by weight mannitol, 10-13 parts by weight magnesium stearate, heating water bath is to 50-60 DEG C, regulation system pH
=7, after stirring 1-2h, heating water bath obtains colloidal solution to 65-75 DEG C；
Gel preparation: colloidal solution, 15-25 parts by weight are uniformly mixed without ammonia natural emulsion, after being heated to 70-80 DEG C, are added
10-15 weight account polyethylene, 1-3 parts by weight of crosslinking agent stir 30-40min with the speed of 200-300rpm, and 50-70 is then added
Parts by weight guar gum, 40-60 parts by weight carbopol, regulation system pH=6 obtain gel after stirring 1-2h；
Medicament preparation: being ventilated with the air velocity of 25L/min to gel, while stirring 20- with the rate of 2000-3000rpm
The part by weight modified chitosan of 40-80 is added after 30min, is taken out after stirring 2-3h, in 60-70 DEG C of microwave drying, crushing obtains medicine
Agent.
6. a kind of preparation method of medicament for preventing and treating ring rot of apple according to claim 5, which is characterized in that described to change
The preparation step of property chitosan is as follows:
Chitosan is added in 2% acetic acid solution, is configured to the chitosan solution of 2-3wt%, 2- chloromethyl benzo miaow is added
Azoles is heated with stirring to 80 DEG C, under nitrogen atmosphere, instills the mixed solution of nitric acid and hydrochloric acid, is cooled to 50 DEG C, and cerous nitrate is added
Ammonium after being stirred to react 10-12h, is added formic acid, ethylene glycol, ethyl alcohol, deionized water, is heated to 70-80 DEG C, after the 18-24h that flows back
It is cooled to room temperature, is cleaned after suction filtration, powdered modification of chitosan is dried to obtain at 40 DEG C.
7. a kind of preparation method of medicament for preventing and treating ring rot of apple according to claim 6, which is characterized in that described to change
Property chitosan preparation step in, mixed solution be by 2wt% nitric acid and 3wt% hydrochloric acid according to 1:1 volume ratio mixing and
At.
8. a kind of preparation method of medicament for preventing and treating ring rot of apple according to claim 7, which is characterized in that described to change
Property chitosan preparation step in, the volume ratio of formic acid and ethylene glycol is 1:0.3.
9. a kind of application method for the medicament for preventing and treating ring rot of apple according to claim 4, which is characterized in that by the medicine
Agent is mixed with water according to the volume ratio of 1:5, stirs into gel, is then applied at the disease for having struck off disease tumor.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111887056A (en) * | 2020-06-30 | 2020-11-06 | 阿克苏优能农业科技股份有限公司 | Sugar core promoting and insect pest preventing apple tree regulating agent and application |
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| CN107082826A (en) * | 2017-03-20 | 2017-08-22 | 浙江工商大学 | A kind of graft modification chitosan microball and its preparation method and application |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111887056A (en) * | 2020-06-30 | 2020-11-06 | 阿克苏优能农业科技股份有限公司 | Sugar core promoting and insect pest preventing apple tree regulating agent and application |
| CN111887056B (en) * | 2020-06-30 | 2022-11-01 | 阿克苏优能农业科技股份有限公司 | Sugar core promoting and insect pest preventing apple tree regulating agent and application |
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| CN109645012B (en) | 2021-03-30 |
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