CN109619306B - Weaned piglet mitochondrial function protective agent - Google Patents

Weaned piglet mitochondrial function protective agent Download PDF

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Publication number
CN109619306B
CN109619306B CN201910107495.0A CN201910107495A CN109619306B CN 109619306 B CN109619306 B CN 109619306B CN 201910107495 A CN201910107495 A CN 201910107495A CN 109619306 B CN109619306 B CN 109619306B
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mitochondrial
syringin
curcumin
piglets
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CN109619306A (en
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胡彩虹
曹舒婷
冯杰
王春春
肖勘
宋泽和
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Zhejiang University ZJU
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings

Abstract

The invention discloses a weaned piglet mitochondrial function protective agent, which comprises the following components: 0.3-1.5 parts of ellagic acid, 0.3-2 parts of curcumin, 0.3-1.3 parts of syringin and 3-15 parts of sodium montmorillonite. Compared with the feed which is added with ellagic acid, curcumin, syringin and sodium montmorillonite alone, the mitochondrial function protective agent for weaned piglets provided by the invention can be used for remarkably improving the contents of intestinal tract and liver mitochondrial DNA, the activity of mitochondrial respiratory chain compound IV and the expression of mitochondrial autophagy marker proteins PINK1, Parkin and LC3II/LC3I of weaned piglets. Ellagic acid, curcumin, syringin and sodium montmorillonite have significant interaction in improving functions of mitochondria of intestinal tract and liver and mitochondrial autophagy level, and have positive synergistic combination effect. The mitochondrial function protective agent for weaned piglets can obviously improve the autophagy level of intestinal tracts and liver mitochondria after weaning and greatly improve the mitochondrial functions of the intestinal tracts and the liver mitochondria after weaning through the synergistic effect of all the components.

Description

Weaned piglet mitochondrial function protective agent
Technical Field
The invention relates to a mitochondrial function protective agent for weaned piglets.
Background
The early weaning technology of the piglets is widely applied to the contemporary pig raising industry, because the early weaning of the piglets can shorten the breeding period of the sows, increase the annual delivery times of the sows and the utilization rate of a delivery stall, and can also reduce the vertical spread of maternal diseases to the piglets, improve the health condition of the piglets and improve the growth performance. However, the digestive system of early weaned pigs is not developed to be mature, and the weaned stress often causes the digestive function disorder, the disease resistance of the piglets to be weakened, the growth to be retarded, and even the weaned pigs to die, thereby causing great economic loss to the pig industry.
The weaning of piglets induces complex morphological and functional changes involving nutrition, digestive metabolism, stress, neuroendocrine, gene function, etc. Weaning is the biggest stress of piglets after birth, the feed of weaning piglets is changed, the intestines and stomach of the piglets cannot be adapted, anorexia and dyspepsia occur, the weight of the piglets is reduced, skeletal muscles closely related to the production performance and the later-stage production traits also have certain degenerative changes in the later-stage development process, and further the growth inhibition is shown. Meanwhile, weaning stress can cause the barrier function of intestinal tracts of piglets to be damaged, which is represented by the change of mucosal morphological structure, the increase of intestinal epithelial barrier permeability, the reduction of digestive absorption function, the reduction of mucus layer thickness, the increase of intestinal tract PH, immunosuppression, the unbalance of intestinal microbial flora and the like, and the damaged intestinal barriers further activate a plurality of signal paths related to the intestinal functions to cause secondary damage and functional disorder of the intestinal functions. Meanwhile, the intestinal tract is not only the main organ for digestion and absorption, but also the largest immune organ of the body, and covers more than 80% of immune cells of the immune system of the body. The intestinal immune system plays a role in immune monitoring and defense of normal intestinal flora and foreign pathogenic bacteria on the basis of maintaining the immune tolerance of the intestinal mucosa to food antigens. After weaning, the intestinal form and function of piglets are changed due to stress such as nutrition and environment, and harmful substances such as bacteria, LPS or toxin in intestinal cavities easily enter intestinal submucosa to trigger abnormal intestinal immune response, so that the immune system of organisms is disordered and serious inflammatory response is triggered.
Recent studies found that weaning leads to impaired mitochondrial function in piglets' intestinal tracts, and a phenomenon of mitochondrial autophagy was also observed. Compensatory mitophagy caused by weaning stress is not enough to repair body damage caused by weaning stress, and no report of relieving weaning stress by regulating mitochondrial function and mitophagy is found so far. However, mitophagy is also a double-edged sword, and excessive or disturbed mitophagy can lead to excessive degradation of mitochondria in cells, cause energy metabolism disorder of cells and even cell death. Therefore, the development of the protective agent for accurately regulating and controlling the mitochondrial function of the weaned piglets has practical application value and wide market prospect for pig breeding production.
Disclosure of Invention
The invention aims to provide a mitochondrial function protective agent for weaned piglets.
A mitochondrial function protective agent for weaned piglets comprises: 0.3-1.5 parts of ellagic acid, 0.3-2 parts of curcumin, 0.3-1.3 parts of syringin and 3-15 parts of sodium montmorillonite.
In the present invention, ellagic acid, curcumin and syringin are obtained by a commercially available method.
The Ellagic acid (Ellagic acid,2,3,7,8-tetrahydroxy benzopyrano5,4, 3-cdebenzapyran-5, 10-dione) has a chemical formula C14H6O8And is commercially available. Ellagic acid is a natural polyphenol component widely present in plant tissues of various soft fruits, nuts, etc. China is a big country for pomegranate production, the annual output of pomegranate is huge, and in the modern fruit juice processing industry, the juice is extracted by squeezing whole pomegranate fruits, and the peel is rich as a waste resource and has low price. Therefore, the ellagic acid can be obtained by degrading macromolecular tannin in pomegranate peel polyphenol with enzyme in the production process.
The curcumin is obtained from extract powder of rhizomes of perennial herb Curcumalonga L of Curcuma of Zingiberaceae. Curcumin can be purchased from markets such as Hebei Tianxu Biotechnology Co., Ltd, Ningbo Chinese medicine pharmacy Co., Ltd, etc.
The syringin is bark extract powder of Syringa vulgaris L of Eugenia of Oleaceae. Syringin is available from the market, such as Chengdu Croma Biotech Co., Ltd, Shanghai Pongjing industry Co., Ltd, etc.
The preparation method of the sodium-based montmorillonite comprises the following steps: adding water into montmorillonite minerals and sodium chloride which is 5-15% of the weight of the minerals, uniformly stirring to prepare suspension ore pulp with the concentration of 10-20%, carrying out sodium treatment for 10-15 hours at room temperature, washing for 5-7 times, and filtering or centrifugally dewatering; and drying, crushing and sieving the obtained filter cake to obtain powdery sodium-based montmorillonite with the mass purity of more than or equal to 90 percent, the blue absorption amount of more than or equal to 40.0g/100g and the particle size (D50) of less than or equal to 10.0 um.
The invention also provides a feed containing the mitochondrial function protective agent for the weaned piglets. The addition amount of the protective agent in the feed is 0.02 wt.% to 0.05 wt.%.
The mitochondrial function protective agent for weaned piglets has the following characteristics:
(1) compared with the feed which is added with ellagic acid, curcumin, syringin and sodium montmorillonite alone, the mitochondrial function protective agent for weaned piglets provided by the invention can be used for remarkably improving the contents of intestinal tract and liver mitochondrial DNA and the activity of a mitochondrial respiratory chain compound IV of weaned piglets and remarkably improving the expression of intestinal tract and liver mitochondrial autophagy marker proteins PINK1, Parkin and LC3II/LC 3I.
(2) Ellagic acid, curcumin, syringin and sodium montmorillonite have significant interaction in improving functions of mitochondria of intestinal tract and liver and mitochondrial autophagy level, and have positive synergistic combination effect.
(3) In addition, the sodium-based montmorillonite has controlled release effect on ellagic acid, curcumin and syringin, so that the sodium-based montmorillonite can be easily mixed with feed to form a uniform dispersion system, and is convenient to use.
(4) The mitochondrial function protective agent for weaned piglets can obviously improve the autophagy level of intestinal tracts and liver mitochondria after weaning, greatly improve the functions of the intestinal tracts and the liver mitochondria after weaning and relieve the functional damage of the intestinal tracts and the liver mitochondria caused by weaning stress through the synergistic effect of all the components.
Detailed Description
The invention is further illustrated by the following examples.
The reagents and materials in the following examples can be prepared by commercially available products or methods reported in the prior art, and are not limited.
Wherein, the preparation method of the sodium montmorillonite in the following embodiments comprises the following steps: adding water into montmorillonite minerals and sodium chloride which is 5-15% of the weight of the minerals, uniformly stirring to prepare suspension ore pulp with the concentration of 10-20%, carrying out sodium treatment for 10-15 hours at room temperature, washing for 5-7 times, and filtering or centrifugally dewatering; and drying, crushing and sieving the obtained filter cake to obtain powdery sodium-based montmorillonite with the mass purity of more than or equal to 90 percent, the blue absorption amount of more than or equal to 40.0g/100g and the particle size (D50) of less than or equal to 10.0 um. The physical and chemical parameters of the sodium montmorillonite can be realized by adjusting the proportion of each component and the preparation process parameters. Of course, in other embodiments, sodium-based montmorillonite may be a commercially available product, with similar effects.
Example l
The obtained sodium-based montmorillonite has a mass purity of 92%, a blue absorption amount of 42g/100g and a particle size (D50) of 6 um.
0.3 kg of ellagic acid, 0.3 kg of curcumin, 0.3 kg of syringin and 3 kg of sodium montmorillonite are mixed and stirred evenly to obtain a weaned piglet mitochondrial function Protective agent (PMF). The additive amount in piglet feed is 0.03 wt.%.
108 21-day-old Du multiplied by long multiplied by big weaned piglets with average weight of 6kg were selected and randomly divided into 6 groups, which are respectively: control group, ellagic acid group, curcumin group, syringin group, sodium montmorillonite group, and PMF group. The control group was fed with conventional piglet feed, the ellagic acid group was fed with piglet feed supplemented with ellagic acid, the curcumin group was fed with piglet feed supplemented with curcumin, the syringin group was fed with piglet feed supplemented with syringin, the sodium-based montmorillonite group was fed with piglet feed supplemented with sodium-based montmorillonite, and the PMF group was fed with piglet feed supplemented with 0.03 wt.% PMF. The content of the components added in the ellagic acid group, the curcumin group, the syringin group and the sodium-based montmorillonite group in the feed is consistent with the content of the corresponding components in the feed of the PMF group. Each group had 3 replicates, each replicate 6 piglets. The test period was 14 days. The piglets are fed and drunk freely, the piglets are induced to feed from the age of 7 days and weaned from the age of 21 days, and the immunization and the feeding management are carried out on the piglets according to the conventional program.
Compared with a control group, an ellagic acid group, a curcumin group, a syringin group and a sodium-based montmorillonite group, the functions of intestinal tracts and liver mitochondria of the piglets in the PMF group are obviously improved, and the DNA content of the intestinal tracts and the liver mitochondria of the piglets and the activity of a mitochondrial oxidation respiratory chain compound IV are obviously increased (P is less than 0.05). Ellagic acid, curcumin, syringin and sodium montmorillonite have significant interaction (P <0.05) in improving the mitochondrial function in intestinal tract and liver, namely the content of mitochondrial DNA in intestinal tract and liver and the activity expression of mitochondrial oxidation respiratory chain complex IV. The results show that PMF obviously improves the functions of the mitochondria of the intestinal tract and the liver.
Compared with a control group, an ellagic acid group, a curcumin group, a syringin group and a sodium-based montmorillonite group, the level of mitochondrial autophagy in intestinal tracts and livers of piglets in the PMF group is obviously increased, and the expression of mitochondrial autophagy marker proteins PINK1, Parkin and LC3II/LC3I in the intestinal tracts and the livers is obviously improved (P is less than 0.05). Ellagic acid, curcumin, syringin and sodium montmorillonite have significant interaction (P <0.05) on improving the level of mitophagy in intestinal tract and liver, namely the expression of mitophagy marker proteins PINK1, Parkin and LC3II/LC 3I. The results show that PMF obviously improves the autophagy level of mitochondria of intestinal tracts and livers.
According to the results, the mitochondrial function protective agent added into the feed for the weaned piglets can obviously protect the functions of the intestinal tracts and the liver mitochondria, improve the autophagy level of the intestinal tracts and the liver mitochondria after the weaning of the piglets and relieve the functional damage of the intestinal tracts and the liver mitochondria caused by weaning stress.
Example 2
The obtained sodium-based montmorillonite has a mass purity of 93%, a blue absorption amount of 46g/100g, and a particle size (D50) of 8 um.
1.5 kg of ellagic acid,2 kg of curcumin, 1.3 kg of syringin and 15 kg of sodium montmorillonite are mixed and stirred evenly to obtain a weaned piglet mitochondrial function Protective agent (PMF). The additive amount in piglet feed is 0.02 wt.%.
108 28-day-old Du multiplied by long multiplied by big weaned piglets with average weight of 7kg are selected and randomly divided into 6 groups, which are respectively: control group, ellagic acid group, curcumin group, syringin group, sodium montmorillonite group, and PMF group. The control group was fed with conventional piglet feed, the ellagic acid group was fed with piglet feed supplemented with ellagic acid, the curcumin group was fed with piglet feed supplemented with curcumin, the syringin group was fed with piglet feed supplemented with syringin, the sodium-based montmorillonite group was fed with piglet feed supplemented with sodium-based montmorillonite, and the PMF group was fed with piglet feed supplemented with 0.02 wt.% PMF. The content of the components added in the ellagic acid group, the curcumin group, the syringin group and the sodium-based montmorillonite group in the feed is consistent with the content of the corresponding components in the feed of the PMF group. Each group had 3 replicates, each replicate 6 piglets. The test period was 14 days. The piglets are fed and drunk freely, the piglets are induced to feed from the age of 7 days and weaned from the age of 28 days, and the immunization and the feeding management are carried out on the piglets according to the conventional program.
Compared with a control group, an ellagic acid group, a curcumin group, a syringin group and a sodium-based montmorillonite group, the functions of intestinal tracts and liver mitochondria of the piglets in the PMF group are obviously improved, and the DNA content of the intestinal tracts and the liver mitochondria of the piglets and the activity of a mitochondrial oxidation respiratory chain compound IV are obviously increased (P is less than 0.05). Ellagic acid, curcumin, syringin and sodium montmorillonite have significant interaction (P <0.05) in improving the mitochondrial function in intestinal tract and liver, namely the content of mitochondrial DNA in intestinal tract and liver and the activity expression of mitochondrial oxidation respiratory chain complex IV. The results show that PMF obviously improves the functions of the mitochondria of the intestinal tract and the liver.
Compared with a control group, an ellagic acid group, a curcumin group, a syringin group and a sodium-based montmorillonite group, the level of mitochondrial autophagy in intestinal tracts and livers of piglets in the PMF group is obviously increased, and the expression of mitochondrial autophagy marker proteins PINK1, Parkin and LC3II/LC3I in the intestinal tracts and the livers is obviously improved (P is less than 0.05). Ellagic acid, curcumin, syringin and sodium montmorillonite have significant interaction (P <0.05) on improving the level of mitophagy in intestinal tract and liver, namely the expression of mitophagy marker proteins PINK1, Parkin and LC3II/LC 3I. The results show that PMF obviously improves the autophagy level of mitochondria of intestinal tracts and livers.
According to the results, the mitochondrial function protective agent added into the feed for the weaned piglets can obviously protect the functions of the intestinal tracts and the liver mitochondria and improve the autophagy level of the intestinal tracts and the liver mitochondria after the weaned piglets, so that the functional damage of the intestinal tracts and the liver mitochondria caused by weaning stress is relieved.
Example 3
The obtained sodium-based montmorillonite has a mass purity of 91%, a blue absorption amount of 51g/100g and a particle size (D50) of 9 um.
0.5 kg of ellagic acid, 0.6 kg of curcumin, 0.5 kg of syringin and 6kg of sodium montmorillonite are mixed and stirred evenly to obtain a weaned piglet mitochondrial function Protective agent (PMF). The additive amount in the piglet feed is 0.05 wt.%.
108 23-day-old 'Du X Long X plus' weaned piglets with average weight of 6.46kg are selected and randomly divided into 6 groups, which are respectively: control group, ellagic acid group, curcumin group, syringin group, sodium montmorillonite group, and PMF group. The control group was fed with conventional piglet feed, the ellagic acid group was fed with piglet feed supplemented with ellagic acid, the curcumin group was fed with piglet feed supplemented with curcumin, the syringin group was fed with piglet feed supplemented with syringin, the sodium-based montmorillonite group was fed with piglet feed supplemented with sodium-based montmorillonite, and the PMF group was fed with piglet feed supplemented with 0.05wt.% PMF. The content of the components added in the ellagic acid group, the curcumin group, the syringin group and the sodium-based montmorillonite group in the feed is consistent with the content of the corresponding components in the feed of the PMF group. Each group had 3 replicates, each replicate 6 piglets. The test period was 14 days. The piglets are fed and drunk freely, the piglets are induced to feed from the age of 7 days and weaned to the age of 23 days, and the immunization and the feeding management are carried out on the piglets according to the conventional program.
Compared with a control group, an ellagic acid group, a curcumin group, a syringin group and a sodium-based montmorillonite group, the functions of intestinal tracts and liver mitochondria of the piglets in the PMF group are obviously improved, and the DNA content of the intestinal tracts and the liver mitochondria of the piglets and the activity of a mitochondrial oxidation respiratory chain compound IV are obviously increased (P is less than 0.05). Ellagic acid, curcumin, syringin and sodium montmorillonite have significant interaction (P <0.05) in improving the mitochondrial function in intestinal tract and liver, namely the content of mitochondrial DNA in intestinal tract and liver and the activity expression of mitochondrial oxidation respiratory chain complex IV. The results show that PMF obviously improves the functions of the mitochondria of the intestinal tract and the liver.
Compared with a control group, an ellagic acid group, a curcumin group, a syringin group and a sodium-based montmorillonite group, the level of mitochondrial autophagy in intestinal tracts and livers of piglets in the PMF group is obviously increased, and the expression of mitochondrial autophagy marker proteins PINK1, Parkin and LC3II/LC3I in the intestinal tracts and the livers is obviously improved (P is less than 0.05). Ellagic acid, curcumin, syringin and sodium montmorillonite have significant interaction (P <0.05) on improving the level of mitophagy in intestinal tract and liver, namely the expression of mitophagy marker proteins PINK1, Parkin and LC3II/LC 3I. The results show that PMF obviously improves the autophagy level of mitochondria of intestinal tracts and livers.
According to the results, the mitochondrial function protective agent added into the feed for the weaned piglets can obviously protect the functions of the intestinal tracts and the liver mitochondria and improve the autophagy level of the intestinal tracts and the liver mitochondria after the weaned piglets, so that the functional damage of the intestinal tracts and the liver mitochondria caused by weaning stress is relieved.

Claims (4)

1. A protective agent for relieving weaning stress reaction of weaned piglets is characterized by comprising the following components: 0.3-1.5 parts of ellagic acid, 0.3-2 parts of curcumin, 0.3-1.3 parts of syringin and 3-15 parts of sodium montmorillonite.
2. The protective agent for relieving weaning stress of weaned pigs according to claim 1, wherein the sodium-based montmorillonite is powdery sodium-based montmorillonite with the mass purity of not less than 90%, the blue absorption amount of not less than 40.0g/100g and the particle size D50 of not more than 10.0 um.
3. A feed comprising the protective agent for alleviating weaning stress of a weaned pig according to claim 1 or 2.
4. The feed of claim 3, wherein the protectant is added to the feed in an amount of 0.02 wt.% to 0.05 wt.%.
CN201910107495.0A 2019-02-02 2019-02-02 Weaned piglet mitochondrial function protective agent Active CN109619306B (en)

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