CN109619101A - A kind of fungicide and its application method embedding matrix for sterilizing lily ball - Google Patents

A kind of fungicide and its application method embedding matrix for sterilizing lily ball Download PDF

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Publication number
CN109619101A
CN109619101A CN201811608322.9A CN201811608322A CN109619101A CN 109619101 A CN109619101 A CN 109619101A CN 201811608322 A CN201811608322 A CN 201811608322A CN 109619101 A CN109619101 A CN 109619101A
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China
Prior art keywords
fungicide
embedding matrix
lily
liquid
application method
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Pending
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CN201811608322.9A
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Chinese (zh)
Inventor
陈丽静
孙红梅
张丽
殷晓娟
马慧
陈水森
刘姝
王宝华
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Shenyang Agricultural University
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Shenyang Agricultural University
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Priority to CN201811608322.9A priority Critical patent/CN109619101A/en
Publication of CN109619101A publication Critical patent/CN109619101A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46N-acyl derivatives
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/08Immunising seed
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/26Oxidation products of dithiocarbamic acid derivatives, e.g. thiuram sulfides

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Agronomy & Crop Science (AREA)
  • Soil Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention provides a kind of for sterilizing the fungicide and its application method of lily ball embedding matrix, belongs to lily ball Cultivating techniques field, the fungicide includes the following parts by weight: 400~600 times of metalaxyl, 0.5~1.5 part of liquid;600~1000 times of thiram, 1~5 part of liquid, wherein metalaxyl is systemic fungicide, can be used for preventing and treating fungal disease;Thiram is protective fungicide, can be used for preventing and treating lily epidemic disease.The present invention has obtained the fungicide with interior suction protective effect effect by compounding metalaxyl and thiram.And by experimental verification, fungicide of the invention has apparent synergistic effect.

Description

A kind of fungicide and its application method embedding matrix for sterilizing lily ball
Technical field
The present invention relates to lily ball Cultivating techniques fields, more particularly to one kind is for sterilizing lily ball embedding matrix Fungicide and its application method.
Background technique
Lily (Lilium spp) is Liliaceae lilium perennial herb bulbous plant, be mainly distributed on east Asia, The Northern Hemisphere Temperate Region in China such as Europe, North America is one of most important Fresh Cutting flower in the whole world
In the prior art, the technology that lily ball deepfreeze breaks bulb suspend mode is used widely, i.e., to bulb into Bulb after embedding is placed in 3~7 DEG C of environment and carries out cryopreservation by row embedding treatment, to break lily ball suspend mode, promotees It sprouts into bulb, improves abloom rate.But in cold storage procedure, since bulb embedding matrix is non-sterile or disinfection is not thorough, So that easily occurring to rot during bulb store and the problems such as root system atrophy, and before cold storage of seedball to the disinfection of embedding matrix at Reason process directly affects cold storage of seedball effect, but the disinfection lily ball that is not exclusively used in domestic at present embeds killing for matrix Microbial inoculum, and other fungicide are more single to the preventive effect of lily ball embedding matrix, thus bactericidal effect is also undesirable.
Summary of the invention
The purpose of the present invention is to provide a kind of fungicide and its users that matrix is embedded for sterilizing lily ball Method, the fungicide have good Disinfection Effect.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of for sterilizing the fungicide of lily ball embedding matrix, the group including following parts by weight Part: 400~600 times of metalaxyl, 0.5~1.5 part of liquid;600~1000 times of thiram, 1~5 part of liquid.
Preferably, the fungicide includes the following parts by weight: 400~600 times of metalaxyl, 0.8~1.2 part of liquid; 600~1000 times of thiram, 2~4 parts of liquid.
Preferably, the dosage form of the metalaxyl and thiram is wettable powder.
The present invention provides the application methods of the fungicide described in above scheme, and steps are as follows:
1) fungicide, turf and water are mixed, obtains embedding matrix;
2) embedding treatment is carried out to lily ball using step 1) the embedding matrix.
Preferably, the mass ratio of fungicide described in step 1), turf and water be 0.5~1.5: 10~100: 20~ 200。
Preferably, the mass percentage of water is 60%~70% in embedding matrix described in step 1).
Preferably, as follows the step of embedding treatment described in step 2): the first plastic film of paving, in first plastics It is alternately laid with embedding matrix and lily ball on film, is finally covered the lily ball of top layer with embedding matrix, with second Plastic film covers embedding matrix.
Preferably, it is described embedding matrix with a thickness of 2~4cm/ layers.
Preferably, there is venthole on second plastic film.
Preferably, the Zhou Jing of the lily ball is 14~18cm.
Beneficial effects of the present invention: the present invention provides a kind of for sterilizing the fungicide of lily ball embedding matrix, packet Include the component of following parts by weight: 400~600 times of metalaxyl, 0.5~1.5 part of liquid;600~1000 times of thiram, 1~5 part of liquid, Middle metalaxyl is systemic fungicide, can be used for preventing and treating fungal disease;Thiram is protective fungicide, can be used for preventing and treating hundred Close epidemic disease.The present invention has obtained the sterilization with interior suction protective effect effect by compounding metalaxyl and thiram Agent.And by experimental verification, fungicide of the invention has apparent synergistic effect.
Specific embodiment
The present invention provides a kind of for sterilizing the fungicide of lily ball embedding matrix, the group including following parts by weight Part: 400~600 times of metalaxyl, 0.5~1.5 part of liquid;600~1000 times of thiram, 1~5 part of liquid.
In the present invention, the fungicide includes 400~600 times of metalaxyl, 0.5~1.5 part of liquid;The metalaxyl it is dilute Releasing multiple is preferably 500 times;The number of the metalaxyl is preferably 1 part;The dosage form of the metalaxyl is preferably wettable powder Agent.In the present invention, the metalaxyl is systemic fungicide, can be used for preventing and treating fungal disease.
In the present invention, the fungicide includes 600~1000 times of thiram, 1~5 part of liquid;The dilution of the thiram Multiple is preferably 800 times;The parts by weight of the thiram are preferably 2~4 parts;The dosage form of the thiram is preferably wettable Pulvis.In the present invention, the thiram is protective fungicide, can be used for preventing and treating lily epidemic disease.
Fungicide of the invention has interior suction protective effect.
The present invention provides the preparation methods of fungicide described in above scheme, and steps are as follows:
Metalaxyl and thiram are mixed, fungicide is obtained;The mixed temperature is preferably 18~26 DEG C, more preferably It is 20 DEG C;The mixed time is preferably 5~20min, preferably 10min;The mixed mode preferably stirs mixed It closes;The revolving speed of the stirring is 60~90rpm.
The present invention provides the application methods of the fungicide described in above scheme, and steps are as follows:
1) fungicide, turf and water are mixed, obtains embedding matrix;
2) embedding treatment is carried out to lily ball using step 1) the embedding matrix.
In the present invention, fungicide, turf and water are mixed, obtains embedding matrix;The turf is preferably Tieling Xifeng Or Denmark's Pin Shi turf;The volume ratio of the fungicide, turf and water is preferably 0.5~1.5: 10~100: 20~200, more Preferably 1: 80: 140;The mass percentage of water is preferably 60%~70% in the embedding matrix, and more preferably 65%; The mixed temperature is preferably 18~26 DEG C, and more preferably 20 DEG C;The mixed time is preferably 10~30min, more excellent It is selected as 20min;The mixed mode is preferably stirred;The revolving speed being stirred is preferably 90~150rpm.
The present invention carries out embedding treatment to lily ball after obtaining embedding matrix, using the embedding matrix.The present invention Specific implementation process in, the step of embedding treatment, is as follows: the first plastic film of paving, on first plastic film It is alternately laid with embedding matrix and lily ball, is finally covered the lily ball of top layer with embedding matrix, it is thin with the second plastics Membrane cover lives to embed matrix;The equipment of the embedding treatment preferably embeds case.
In specific implementation process of the invention, the alternately embedding refers to first spreads thickness embedding on a plastic film Matrix puts one layer of lily ball, repaves one layer of embedding matrix, so alternately, finally will be most upper with embedding matrix until piling The lily ball covering of layer.
In the present invention, the number of plies of the embedding matrix is preferably 3~5 layers, and more preferably 4 layers;The thickness of the embedding matrix Preferably 2~4cm/ layers, more preferably 3cm/ layers of degree;There is venthole on second plastic film;The aperture of the venthole Preferably 1~2cm;The quantity of the venthole is preferably 6~12/dm2, more preferably 8~10/dm2;The lily kind All diameters of ball are preferably 14~18cm, more preferably 16cm.
Below with reference to embodiment to it is provided by the invention it is a kind of for sterilize lily ball embedding matrix fungicide and its Application method is described in detail, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1 is a kind of for sterilizing the fungicide of lily ball embedding matrix
1. 500 times of reagent metalaxyl, 800 times of liquid thiram liquid
2. preparation method by 800 times of liquid of 500 times of liquid of metalaxyl and thiram be in mass ratio 1: 10 ratio at 20 DEG C Under the conditions of be stirred.
Embodiment 2 is a kind of for sterilizing the fungicide of lily ball embedding matrix
In addition to the mass ratio of 500 times of liquid of metalaxyl and 800 times of liquid of thiram is 1.5: 1, other are the same as embodiment 1.
Embodiment 3 is a kind of for sterilizing the fungicide of lily ball embedding matrix
In addition to the mass ratio of 500 times of liquid of metalaxyl and 800 times of liquid of thiram is 1: 2, other are the same as embodiment 1.
Embodiment 4 verifies the bactericidal effect of the fungicide of Examples 1 to 3
1. sample: the preparation of Examples 1 to 3 is respectively experimental group 1, experimental group 2 and experimental group 3;Control group 1 is first frost Clever 500 times of liquid, control group 2 are 800 times of liquid of thiram.
2. indoor biometrics experiment method: using Activities measurement experiment group 1~3 and the medicine of control group 1~3 Agent respectively to lily Phytophthora cactorum mycelia growth inhibiting effect, determine each group medicament to germ mycelial growth inhibition rate 10%~ 90% range.From each medicament to 5 gradient concentrations are designed in 10%~90% range of germ mycelial growth inhibition rate, dilute The Tween 80 aqueous solution that solvent is 0.1%, each group medical fluid then be added separately to melt and be cooled down by 1% volume ratio Graded series concentration pastille culture medium is made into the CA culture medium of room temperature, after sufficiently shaking up, pours into the culture dish (Ф of sterilizing Drug containing tablet, four repetitions of every processing are made in 90mm);Picking Phytophthora cactorum is inoculated in the center of drug containing tablet, is placed in 25 DEG C Constant temperature dark culturing case in culture.Colony diameter is measured using crossing method when colony diameter reaches plate 2/3.With Least square method calculates each medicament to concentration EC50 in the inhibition of pathogen, and calculates co-toxicity coefficient CTC according to the following equation, Compare the virulence of each group medicament.
The co-toxicity of mixture preparation is indicated using the co-toxicity coefficient method of Sun Yunpei:
Mixture preparation theoretical toxicity index TTI=∑ (the toxicity index TI of medicament × its in mixture the medicine effectively at The percentage divided).
It is synergistic effect when co-toxicity coefficient CTC >=120, is summation action when between 80 < CTC < 120, when CTC≤80 For antagonism.
3. experimental result:
The medicament of experimental group and control group 1 and 2 is shown in Table 1 to the measurement result of lily Phytophthora cactorum raw test.
Measurement result of the medicament of the different groups of table 1 to lily Phytophthora cactorum raw test
As seen from the results in Table 1, the co-toxicity coefficient CTC of the preparation of Examples 1 to 3 is all larger than 120, it is seen then that of the invention kills Microbial inoculum has apparent synergistic effect.
Embodiment 5 verifies the bactericidal effect of the fungicide of Examples 1 to 3 in actual application
Sample
The preparation of Examples 1 to 3 is respectively experimental group 1, experimental group 2 and experimental group 3;Control group 1 is 500 times of metalaxyl Liquid, control group 2 are 800 times of liquid of thiram, and blank group is water.
Application process
1. bulb selects: after taking the first batch of lily fresh flower harvesting of the middle ten days and the last ten days October current year, lily bulb Zhou Jing being selected to be greater than 14 centimetres or more, cutting root, there are healthy bulb 600 of 3 centimeter length fleshy roots.
2. bulb sterilizes: lily ball surface soil being cleaned, bulb then will be cleaned and is packed into the nylon string bag It is interior, using 40% carbendazim, 500 times of liquid to lily ball soaking disinfection 12h, the bulb after disinfection is randomly divided into 6 groups.
3. matrix treatments: take respectively 800 times of liquid of 500 times of liquid of preparation, metalaxyl and thiram of 10mL Examples 1 to 3 with The mixing of 800mL turf, stirs evenly, then water drenching 1400mL stirring, to be finally sufficiently mixed uniformly, embedding matrix is prepared.
4. bulb embeds: being carried out at embedding using the different embedding matrix being prepared to the lily ball after disinfection Reason, first spreads layer of plastic film in embedding bottom portion, and the embedding matrix that then paving a layer thickness is 3cm on a plastic film is put One layer of matrix is spread after one layer of bulb again, alternately, until filling, top spreads one layer of embedding matrix, the very useful plastic film of case It obturages, punching marks on case for ventilating on plastic film.
5. bulb cold treatment: the bulb handled well being put into 4 DEG C of cold storage at constant temperature, 6 weeks breaking dormancies of cold treatment, 100 days After be colonized.
6. experimental plot is divided into 6 groups, respectively the lily corresponding with the medicament of control group 1,2 of planting experiment group 1~3 Bulb.Seedlings investigation lily epidemic disease diseased plant rate, and preventive effect is calculated, it the results are shown in Table 2.As the result is shown: using the embodiment of the present invention 1 After~3 chemicals treatment lily embedding matrix, 500 times of liquid of metalaxyl and Fu Mei are apparently higher than to the control efficiency of lily epidemic disease Double 800 times of liquid preventive effects.As it can be seen that compound drug of the present invention has preferable preventive effect to lily epidemic disease.
Table 2, different agents are to the control efficiency of lily epidemic disease
Group Diseased plant number Diseased plant rate Preventive effect
Experimental group 1 7 7% 61.1%
Experimental group 2 6 6% 66.7%
Experimental group 3 4 4% 77.8%
Control group 1 9 9% 50%
Control group 2 12 12% 33.3%
Blank group 18 18% /
As seen from the above embodiment, the present invention provides a kind of for sterilizing the fungicide of lily ball embedding matrix, should Fungicide has apparent synergistic effect, and has good preventive effect to lily epidemic disease.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications It should be regarded as protection scope of the present invention.

Claims (10)

1. a kind of fungicide for embedding matrix for sterilizing lily ball, includes the following parts by weight: metalaxyl 400~ 600 times 0.5~1.5 part of liquid;600~1000 times of thiram, 1~5 part of liquid.
2. fungicide according to claim 1, which is characterized in that the fungicide includes the following parts by weight: first White 400~600 times 0.8~1.2 part of liquid of spirit;600~1000 times of thiram, 2~4 parts of liquid.
3. fungicide according to claim 1 or 2, which is characterized in that the dosage form of the metalaxyl and thiram is wettable Property pulvis.
4. the application method of fungicide described in claims 1 to 3 any one, steps are as follows:
1) fungicide, turf and water are mixed, obtains embedding matrix;
2) embedding treatment is carried out to lily ball using step 1) the embedding matrix.
5. application method according to claim 4, which is characterized in that the matter of fungicide described in step 1), turf and water Amount is than being 0.5~1.5: 10~100: 20~200.
6. application method according to claim 4 or 5, which is characterized in that the matter of water in embedding matrix described in step 1) Measuring percentage composition is 60%~70%.
7. application method according to claim 4, which is characterized in that as follows the step of embedding treatment described in step 2): The first plastic film is spread, embedding matrix and lily ball are alternately laid on first plastic film, finally with embedding matrix The lily ball of top layer is covered, covers embedding matrix with the second plastic film.
8. application method according to claim 7, which is characterized in that it is described embedding matrix with a thickness of 2~4cm/ layers.
9. application method according to claim 7, which is characterized in that have venthole on second plastic film.
10. application method according to claim 4, which is characterized in that the Zhou Jing of the lily ball is 14~18cm.
CN201811608322.9A 2018-12-27 2018-12-27 A kind of fungicide and its application method embedding matrix for sterilizing lily ball Pending CN109619101A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116439239A (en) * 2023-04-26 2023-07-18 沈阳农业大学 Lily seed bulb disinfection preparation and preparation method and use method thereof

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