CN109601752A - A kind of special composite enzyme improving 817 meat chicken growth performances - Google Patents
A kind of special composite enzyme improving 817 meat chicken growth performances Download PDFInfo
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- CN109601752A CN109601752A CN201811638149.7A CN201811638149A CN109601752A CN 109601752 A CN109601752 A CN 109601752A CN 201811638149 A CN201811638149 A CN 201811638149A CN 109601752 A CN109601752 A CN 109601752A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
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Abstract
The present invention relates to enzyme applied technical fields, more particularly, to a kind of special composite enzyme for improving 817 meat chicken growth performances.The special composite enzyme of 817 meat chicken growth performance of raising includes zytase component, mannase component, protease component, amylase component, 1,4 beta-glucanase；Alpha-galactosidase, glucose oxidase, lipase.The special composite enzyme of 817 meat chicken growth performance of raising is directed to the physilogical characteristics and nutritional need development of 817 broiler chicken, using single enzyme combination of the same race in different strain source, and phase same-action, the single enzyme of combination of different nature are compounded, prepare complex enzyme, reach and has improved 817 feed digestion utilization rates, growth performance, the purpose of death and culling rate is reduced, there is significant economic benefit.
Description
Technical field
The present invention relates to enzyme applied technical fields, more particularly, to a kind of specific complex for improving 817 meat chicken growth performances
Enzyme.
Background technique
817 cross-broilers are the small-sized meat chicken breeds with local characteristic, have and grow fast, price of deed height, meat flavour
Excellent, the features such as fiber is delicate, is relatively suitble to the taste of domestic consumer, and converted products is delicious, be production braised chicken, roast chicken,
The preferred raw material of smoked chicken, it is quite popular with consumers.The market demand of the kind and up-trend year by year, mesh is presented with the number of animals raised
The year before last amount of delivering for sale about 1,200,000,000 has good market prospects.But domestic broiler chicken market is with fast large-scale broiler chicken and yellow-feather broiler
Based on, since 817 is different in terms of physilogical characteristics and nutritional need, feed digestion utilization rate is to be improved；In addition, feed mold
The chicken proventriculitis of equal initiations is frequent, affects the growth performance of 817 broiler chicken.Complex enzyme formulation has in terms of improving feed digestibility
Certain effect, still, current broiler chicken complex enzyme both domestic and external mainly carry out correlation as object using fast large-scale white meat-type chickens and grind
Study carefully, not specifically for the special composite enzyme of 817 broiler chicken.
The prior art large-scale broiler chicken complex enzyme fastly, specific aim is poor for 817, cannot effectively improve 817 broiler fodders
Digestibility, and existing fast large-scale broiler chicken complex enzyme mostly use be several single enzymes combination, such as cellulase+zytase+
Dextranase+mannase；Or zytase+galactosidase+cellulase+mannase+amylase+lipase
+ protease+pectase since this single enzyme combination is mostly is compounded according to the result of test of many times, this for 817 broiler chicken
It is formulated and is not suitable for.The reason is that 817 is different from fast large-scale broiler chicken in terms of physilogical characteristics and nutritional need, Diet Formula is different,
It needs to provide special composite enzyme for 817, improves its feed digestion utilization rate and premunition.The present invention is quasi- to be developed one kind and is directed to
817 broiler chicken special composite enzyme is answered using the single enzyme of the same race and the single enzyme of phase same-action combination of different nature of separate sources
Match, carry out complex enzyme production according to 817 broiler fodders, reach and improve 817 feed digestion utilization rates, growth performance, reduces dead wash in a pan
The purpose of rate.
Summary of the invention
The present invention provides a kind of special composite enzymes for improving 817 meat chicken growth performances, dedicated for 817 broiler fodders,
With growth promotion, feed digestion utilization rate, improvement growth performance are improved, the effect of death and culling rate is reduced, solves in the prior art
There are the problem of.
Used technical solution is the present invention to solve above-mentioned technical problem:
A kind of special composite enzyme improving 817 meat chicken growth performances, including following enzyme component: zytase component 25000-
30000U/g, mannase component 2700-2900U/g, protease component 11000-15000U/g, amylase component 6000-
6800U/g, 1,4 beta-glucanase 4500-5000U/g；Alpha-galactosidase 80-150U/g, glucose oxidase 300-1800U/g,
Lipase 1000-1500U/g.
The zytase component presses 1:5-7 weight by the zytase of Aspergillus niger origin and the zytase of yeast sources
Than being made；The mannase component presses 1:1.5-2.5 weight ratio system by acid mannase and neutral mannase
At；The protease component is made of acid protease, neutral proteinase, keratinase by 1:1-2:0.5-2 weight ratio；It is described
Combination amylase component is made of mesophilicα-diastase, general Shandong indigo plant enzyme, carbohydrase by 1:10-13:0.5-1.5 weight ratio.
Zytase component is numbered by aspergillus niger-source strain number CICC2462 zytase and yeast-strain
The zytase in the source GS115 is made by 1:5-7 weight ratio.
Application of the special composite enzyme for improving 817 meat chicken growth performances in the preparation of 817 daily ration of broiler feeds.
Additive amount of the complex enzyme in 817 daily ration of broiler feeds is 180-620g/ tons；It is preferred that additive amount is 600g/
Ton.
Beneficial effects of the present invention:
The special composite enzyme of 817 meat chicken growth performance of raising is directed to the physilogical characteristics of 817 broiler chicken and nutritional need is ground
System is compounded using single enzyme combination of the same race in different strain source and phase same-action, the single enzyme of combination of different nature, is made
Standby complex enzyme, has reached and has improved 817 feed digestion utilization rates, growth performance, reduced the purpose of death and culling rate, has significant economical
Benefit.
Specific embodiment
In order to clarify the technical characteristics of the invention, being explained in detail below by specific embodiment the present invention
It states.
Embodiment 1
The special composite enzyme of 817 meat chicken growth performance of raising includes following enzyme component: zytase component 27000U/g,
Mannase component 2800U/g, protease component 13000U/g, amylase component 6400U/g, 1,4 beta-glucanase 4800U/g；
Alpha-galactosidase 115U/g, glucose oxidase 1000U/g, lipase 1200U/g；
The zytase component is compiled by aspergillus niger-source strain number CICC2462 zytase and yeast-strain
The zytase in number source GS115 is made by 1:6 weight ratio；
The mannase component is made of acid mannase and neutral mannase by 1:2 weight ratio；
The protease component is made of acid protease, neutral proteinase, keratinase by 1:1:1 weight ratio；
The combination amylase component is made of mesophilicα-diastase, general Shandong indigo plant enzyme, carbohydrase by 1:12:1 weight ratio.
Embodiment 2
The special composite enzyme of 817 meat chicken growth performance of raising includes following enzyme component: zytase component 25000U/g,
Mannase component 2700U/g, protease component 11000U/g, amylase component 6000U/g, 1,4 beta-glucanase 4500U/g；
Alpha-galactosidase 80U/g, glucose oxidase 300U/g, lipase 1000U/g；
The zytase component is compiled by Aspergillus niger origin-strain number CICC2462 zytase and yeast-strain
The zytase in number source GS115 is made by 1:5 weight ratio；
The mannase component is made of acid mannase and neutral mannase by 1:1.5 weight ratio；
The protease component is made of acid protease, neutral proteinase, keratinase by 1:1:0.5 weight ratio；
The combination amylase component is made of mesophilicα-diastase, general Shandong indigo plant enzyme, carbohydrase by 1:10:0.5 weight ratio.
Embodiment 3
The special composite enzyme of 817 meat chicken growth performance of raising includes following enzyme component: zytase component 30000U/g,
Mannase component 2900U/g, protease component 15000U/g, amylase component 6800U/g, 1,4 beta-glucanase 5000U/g；
Alpha-galactosidase 150U/g, glucose oxidase 1800U/g, lipase 1500U/g；
The zytase component is compiled by aspergillus niger-source strain number CICC2462 zytase and yeast-strain
The zytase in number source GS115 is made by 1:7 weight ratio；
The mannase component is made of acid mannase and neutral mannase by 1:2.5 weight ratio；
The protease component is made of acid protease, neutral proteinase, keratinase by 1:2:2 weight ratio；
The combination amylase component is made of mesophilicα-diastase, general Shandong indigo plant enzyme, carbohydrase by 1:13:1.5 weight ratio.
Evaluation test:
One, daily grain of chicken feed is added in the special composite enzyme of each embodiment and fast large-scale complex enzyme, to the feed of 817 broiler chicken
Effect compares test
The fast large-scale broiler chicken complex enzyme of excellent effect on the market: complex enzyme A, complex enzyme B, complex enzyme C, complex enzyme D is selected,
The specially precious and U.S. ferment of sieve enzyme, overflow multienzyme P-8601, poultry special composite enzyme 512RA and 1-3 of the embodiment of the present invention
Special composite enzyme；Based on 817 daily rations, external hydrolysis result and animal experiment are carried out, verifies the dedicated of the embodiment of the present invention
Feeding effect of the complex enzyme to 817 broiler chicken.
1, concrete operation method: external enzymatic hydrolysis experiment weighs 1.0g (± 0.0002g) feedstuff and (crosses 60 meshes, everywhere
Manage 3 repetitions) in 50ml centrifuge tube, 8mL simulate the gastric juice is added, 1% antibiotic of 1ml, to be rapidly added 1mL dilute in oscillation
It releases to the enzyme solution of corresponding enzyme activity and vibrates 4h in 41 DEG C of water-baths after mixing.After keeping the temperature 4h, 6mL is rapidly added in centrifuge tube
Small intestine buffer, 1.6mL simulated intestinal fluid continue 41 DEG C of constant temperature water bath oscillation heat preservation 15h.It is centrifuged 10min, takes supernatant with pipettor
The spare measurement reduced sugar of liquid.30mL deionized water is added in remaining residue, 10min is centrifuged after mixing, outwells supernatant, weight
Again twice, 95% ethyl alcohol of 20mL is then added to wash in the same way three times.The centrifuge tube for filling filter residue is finally placed in 60
DEG C thermostatic drying chamber dries 12h, then is transferred to 105 DEG C of thermostatic drying chambers drying to constant weight, and electronic analytical balance is weighed for calculating
Dry matter digestibility.
Measuring project such as table 1, experimental result such as table 2.
The external enzymatic hydrolysis experimental design of the 817 broiler chicken special composite enzymes of the invention of watch 1 and fast large-scale broiler chicken complex enzyme
The external hydrolysis result of the 817 broiler chicken special composite enzymes of the invention of watch 2 and fast large-scale broiler chicken complex enzyme
As shown in Table 2,817 broiler chicken specific complex enzyme effects of the embodiment of the present invention are more preferable, the embodiment of the present invention 1 it is dedicated
After complex enzyme zymohydrolysis content of reducing sugar than existing fast large size broiler chicken complex enzyme A, B, C, D be respectively increased 5.88%, 2.26%,
11.22%, 16.04%, dry matter digestibility is respectively increased 19.76% than existing fast large size broiler chicken complex enzyme A, B, C, D,
16.57%, 24.37%, 30.14%；Content of reducing sugar is than existing fast large size after the special composite enzyme enzymatic hydrolysis of the embodiment of the present invention 2
Broiler chicken complex enzyme A, B, C, D are respectively increased 6.78%, 3.13%, 12.16%, 17.03%；Dry matter digestibility is fast bigger than existing
Type broiler chicken complex enzyme A, B, C, D are respectively increased 23.03%, 19.80%, 27.76%, 33.70%；The embodiment of the present invention 3 it is special
Be respectively increased 6.78% than existing fast large size broiler chicken complex enzyme A, B, C, D with content of reducing sugar after complex enzyme zymohydrolysis, 3.13%,
12.16%, 17.03%；Dry matter digestibility is respectively increased 23.03% than existing fast large size broiler chicken complex enzyme A, B, C, D,
19.75%, 27.76%, 33.70%.As it can be seen that being compared with fast large-scale broiler chicken complex enzyme, the special composite enzyme of the embodiment of the present invention
Significantly improve content of reducing sugar and dry matter digestibility.
2, zoopery is designed using single factor experiment, is randomly selected 817 broiler chicken of healthy 0 age in days, is divided into 7 processing groups,
Such as the following table 3.3 repetitions of each processing group, 66 chickens of each repetition amount to 1386 chickens.Test daily ration and trophic level are shown in Table
4.Experimental result is shown in Table 5.
The animal experiment of the 817 broiler chicken special composite enzymes of the invention of watch 3 and fast large-scale broiler chicken complex enzyme designs
Table 4 tests daily ration and trophic level
Note: a: premix provides for every kg daily ration: iron 82mg；Copper 10.5mg；Manganese 60.5mg；Zinc 40.2mg；Iodine 0.36mg；
Selenium 0.16mg；VA 1520 IU；VD3406 IU；VE 52 IU；VK34.1mg；Thiamine (VB1)6.2mg；Riboflavin (VB2)
12.1mg；Pyridoxol (VB6)6.1mg；Cyanocobalamin (VB12)0.05mg；Biotin 0.21mg；Folic acid 2.1mg；Niacin
50.5mg；Calcium pantothenate 25.2mg.
B: the nutritive water level values in table are calculated value.
Influence (the 1- of the 817 broiler chicken special composite enzymes of the invention of watch 5 and fast large-scale broiler chicken complex enzyme to 817 growth performances
52d)
As shown in Table 5, the special composite enzyme significant effect of the embodiment of the present invention.To ADFI, 817 broiler chicken of embodiment 1 are special
Be respectively increased 5.81%, 1.27%, 2.26%, 4.13% than complex enzyme A, B, C, D with complex enzyme, embodiment 2 than complex enzyme A,
B, C, D are respectively increased 6.55%, 1.99%, 2.98%, 4.86%, embodiment 3 is respectively increased 5.5% than complex enzyme A, B, C, D,
0.98%, 1.97%, 3.83%；To ADG, 817 broiler chicken special composite enzymes of embodiment 1 are respectively increased than complex enzyme A, B, C, D
13.27%, 9.58%, 9.33%, 9.83%, embodiment 2 is respectively increased 12.77% than complex enzyme A, B, C, D, 9.09%,
8.84%, 9.34%, embodiment 3 is respectively increased 11.69%, 8.05%, 7.80%, 8.30% than complex enzyme A, B, C, D；To F/
G, 817 broiler chicken special composite enzymes of embodiment 1 reduce by 6.59% than complex enzyme A, B, C, D respectively, 7.69%, 7.14%,
5.45%；Embodiment 2 reduces by 5.39%, 6.51%, 5.95%, 4.24% than complex enzyme A, B, C, D respectively, and embodiment 3 is than multiple
Synthase A, B, C, D reduce by 5.39%, 6.51%, 5.95%, 4.24% respectively；To weight is delivered for sale, 817 broiler chicken of embodiment 1 are special
Be respectively increased 13.38%, 9.88%, 9.20%, 9.88% than complex enzyme A, B, C, D with complex enzyme, embodiment 2 than complex enzyme A,
B, C, D are respectively increased 12.74%, 9.26%, 8.59%, 9.26%, and embodiment 3 is respectively increased than complex enzyme A, B, C, D
12.10%, 8.64%, 7.98%, 8.64%；To survival rate, 817 broiler chicken special composite enzymes of embodiment 1 than complex enzyme A, B,
C, D is respectively increased 6.45%, 3.13%, 2.06%, 3.13%, 817 broiler chicken special composite enzymes of embodiment 2 than complex enzyme A, B,
C, D is respectively increased 6.24%, 2.92%, 1.86%, 2.92%, 817 broiler chicken special composite enzymes of embodiment 3 than complex enzyme A, B,
C, D is respectively increased 5.91%, 2.60%, 1.55%, 2.60%.By result as it can be seen that being compared with fast large-scale complex enzyme, embodiment 1
817 broiler chicken special composite enzymes significantly improve production performance.
Two, the effect test of different gradients of 817 special composite enzymes of the invention on 817 broiler chicken
Test is divided into external proteolysis assay and animal experiment.External enzymatic hydrolysis experiment, experimental design are shown in Table 6, and test result is shown in
Table 7.
The external enzymatic hydrolysis experimental design of 817 special composite enzymes of the invention of watch 6 and fast large-scale broiler chicken complex enzyme
The external hydrolysis result of the special composite enzyme of the present invention of watch 7 and fast large-scale broiler chicken complex enzyme
As shown in Table 7, external hydrolysis result, 817 broiler chicken special composite enzyme additive amounts are in 600g/t, dry matter digestibility
With content of reducing sugar highest, exceed 600g/t, dry matter digestibility and content of reducing sugar promotion be not significant, examines from economic and cost
Consider, additive amount 600g/t is best, and 400g/t takes second place, poor lower than 200g/t.
Animal experiment is carried out to the special composite enzyme of embodiment 2 to design using single factor experiment, randomly selects healthy 0 age in days
817 broiler chicken are divided into 5 processing groups, are specifically shown in Table 9,3 repetitions of each processing group, 66 chickens of each repetition amount to 990 chickens.
Test daily ration and trophic level are shown in Table 4.Test result is shown in Table 10.
9 experimental design of table
Influence (1-52d) of the table 10 to 817 growth performances
As shown in Table 10, when adding 600g/t in daily ration, the daily gain highest of 817 broiler chicken, feedstuff-meat ratio are minimum, exceed
600g/t, daily gain and average daily gain promotion be not significant, and feedstuff-meat ratio also reduces not significantly, from economic and cost consideration, adds
Dosage 600g/t is best, and 400g/t takes second place, poor lower than 200g/t.Animal test results are consistent with digesting test result in vitro.
As it can be seen that the optimum addition of 817 broiler chicken special composite enzymes is 600g/t in daily ration.
Three, Economic and Efficiency Analysis
Average value with fast large-scale complex enzyme (A, B, C, D) growth performance is control, 817 broiler chicken special composite enzyme 200g/
T, the economic benefit that 400g/t, 600g/t are generated in contrast, is specifically shown in Table 11.By table as it can be seen that 817 broiler chicken complex enzymes are from 200-
600g/t different additive amounts, to 10,000 817 broiler chicken fields, every batch of chicken is created more than fast large-scale complex enzyme currently on the market
6800-16900 member is made, about 20,000-5 ten thousand (being calculated with 1 year 3 batches of chicken) can be created within 1 year.
Table 11
Above-mentioned specific embodiment cannot function as limiting the scope of the invention, for the technology people of the art
For member, any alternate modification or transformation made to embodiment of the present invention are fallen within the scope of protection of the present invention.
Place is not described in detail by the present invention, is the well-known technique of those skilled in the art of the present technique.
Claims (5)
1. a kind of special composite enzyme for improving 817 meat chicken growth performances, which is characterized in that including following enzyme component: zytase
Component 25000-30000U/g, mannase component 2700-2900U/g, protease component 11000-15000U/g, amylase
Component 6000-6800U/g, 1,4 beta-glucanase 4500-5000U/g；Alpha-galactosidase 80-150U/g, glucose oxidase
300-1800U/g, lipase 1000-1500U/g.
2. a kind of special composite enzyme for improving 817 meat chicken growth performances according to claim 1, which is characterized in that described
Zytase component is made of the zytase of Aspergillus niger origin and the zytase of yeast sources by 1:5-7 weight ratio；
The mannase component is made of acid mannase and neutral mannase by 1:1.5-2.5 weight ratio；
The protease component is made of acid protease, neutral proteinase, keratinase by 1:1-2:0.5-2 weight ratio；
The combination amylase component presses 1:10-13:0.5-1.5 weight ratio system by mesophilicα-diastase, general Shandong indigo plant enzyme, carbohydrase
At.
3. a kind of special composite enzyme for improving 817 meat chicken growth performances according to claim 1 or 2 is in 817 daily ration of broiler
Application in feed preparation.
4. application according to claim 3, which is characterized in that addition of the complex enzyme in 817 daily ration of broiler feeds
Amount is 180-620g/ tons.
5. application according to claim 3, which is characterized in that addition of the complex enzyme in 817 daily ration of broiler feeds
Amount is 600g/ tons.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628747A (en) * | 2019-08-30 | 2019-12-31 | 广东溢多利生物科技股份有限公司 | Combined amylase and application thereof |
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|---|---|---|---|---|
| US5817500A (en) * | 1995-01-26 | 1998-10-06 | Novo Nordisk A/S | Animal feed additives |
| CN101874547A (en) * | 2009-11-27 | 2010-11-03 | 济南天天香有限公司 | Complex enzyme for feed |
| CN105309773A (en) * | 2015-03-26 | 2016-02-10 | 上海欧耐施生物技术有限公司 | Complex enzyme improving broiler chicken breeding performance and application |
| CN107927382A (en) * | 2017-10-30 | 2018-04-20 | 山东弘德农牧科技有限公司 | A kind of complex enzyme preparation for feeding and preparation method and application |
-
2018
- 2018-12-29 CN CN201811638149.7A patent/CN109601752A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5817500A (en) * | 1995-01-26 | 1998-10-06 | Novo Nordisk A/S | Animal feed additives |
| CN101874547A (en) * | 2009-11-27 | 2010-11-03 | 济南天天香有限公司 | Complex enzyme for feed |
| CN105309773A (en) * | 2015-03-26 | 2016-02-10 | 上海欧耐施生物技术有限公司 | Complex enzyme improving broiler chicken breeding performance and application |
| CN107927382A (en) * | 2017-10-30 | 2018-04-20 | 山东弘德农牧科技有限公司 | A kind of complex enzyme preparation for feeding and preparation method and application |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628747A (en) * | 2019-08-30 | 2019-12-31 | 广东溢多利生物科技股份有限公司 | Combined amylase and application thereof |
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