CN109601376B - Clone plant functional character observation device and operation method - Google Patents
Clone plant functional character observation device and operation method Download PDFInfo
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- CN109601376B CN109601376B CN201811244589.4A CN201811244589A CN109601376B CN 109601376 B CN109601376 B CN 109601376B CN 201811244589 A CN201811244589 A CN 201811244589A CN 109601376 B CN109601376 B CN 109601376B
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- 238000000034 method Methods 0.000 title claims abstract description 9
- 238000009423 ventilation Methods 0.000 claims abstract description 43
- 238000005286 illumination Methods 0.000 claims abstract description 19
- 238000007789 sealing Methods 0.000 claims description 89
- 238000004891 communication Methods 0.000 claims description 17
- 230000009471 action Effects 0.000 claims description 11
- 230000003287 optical effect Effects 0.000 claims description 7
- 230000007704 transition Effects 0.000 claims description 4
- 230000005540 biological transmission Effects 0.000 claims description 2
- 238000012258 culturing Methods 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 40
- 239000000243 solution Substances 0.000 description 5
- 238000009434 installation Methods 0.000 description 4
- 210000000056 organ Anatomy 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 3
- 238000002834 transmittance Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 244000261422 Lysimachia clethroides Species 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000010254 physiological adaptation Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000003938 response to stress Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The device and the operation method for observing the functional properties of the cloned plants are used for conveniently culturing and observing the cloned plants. The culture flask comprises an upper cover, a culture flask, a support rod, an illumination unit, a ventilation unit, a guide pipe, an adjusting unit and a measuring tape, wherein the upper cover is arranged on the bottle mouth of the culture flask, a round hole is formed in the center of the upper cover, the vertically placed measuring tape is arranged in the culture flask, the support rod is detachably arranged on the side wall of the upper cover, and the illumination unit with adjustable illumination intensity is arranged on the support rod; the top of the upper cover is provided with a ventilation unit, and the ventilation unit comprises a ventilation box and a ventilation pipe; when the air exchanging box is compressed, the air in the culture bottle is discharged through the air exchanging pipe; when the ventilation box is lifted, external air enters the ventilation box through the ventilation pipe. The outer wall of the upper cover is fixedly provided with a guide pipe, the guide pipe is provided with a left conveying pipe and a right conveying pipe, and an adjusting unit for adjusting the opening and closing states and opening sizes of the left conveying pipe and the right conveying pipe is arranged in the guide pipe. The invention can conveniently realize the observation and culture of the cloned plants.
Description
Technical Field
The invention relates to the technical field of cloned plant growth observation, in particular to a cloned plant functional character observation device and an operation method.
Background
The cloned plants are plant groups which realize population growth through asexual propagation, and the clone organs such as buds, tillers or branches connected with the parent plant generate propagules, so that important economic value and ecological functions are exerted, and the cloned plants have important significance for resource protection and habitat restoration. The research of the functional characters of the cloned plants is an important way for revealing the physiological adaptation strategy and stress response mechanism of the individual plants. At present, research on the functional properties of the cloned plants is generally carried out by collecting plant individuals and tissue organs for observation and measurement, and the stress resistance and the anti-interference capability of the functional organs of the cloned plants are weak, so that the observation mode of collecting the plant individuals or the tissue organs not only damages the cloned plants, but also causes the resource waste thereof, and is not beneficial to carrying out long-term dynamic observation. Therefore, there is an urgent need for a device for observing the functional properties of a cloned plant, so as to realize in-situ nondestructive observation and sustainable dynamic research on the functional properties of the cloned plant.
Disclosure of Invention
The invention aims to provide a cloned plant functional character observation device and an operation method, which are used for conveniently culturing and observing cloned plants.
The technical scheme adopted for solving the technical problems is as follows: the clone plant functional character observation device is characterized by comprising an upper cover, a culture bottle, a support rod, an illumination unit, a ventilation unit, a guide pipe, an adjusting unit and a measuring scale, wherein the upper cover is arranged on the bottle mouth of the culture bottle in a threaded manner, a round hole for placing plant stems is formed in the center of the upper cover, the measuring scale which is vertically placed is arranged in the culture bottle, the support rod is detachably arranged on the side wall of the upper cover, and the illumination unit with adjustable illumination intensity is arranged on the support rod;
the top of the upper cover is provided with a ventilation unit, the ventilation unit comprises a ventilation box and a ventilation pipe, the ventilation box comprises an upper plate, a telescopic pipe and a lower plate which are sequentially arranged from top to bottom, the lower plate is in threaded connection with the top of the upper cover, the lower plate and the top of the upper cover are well sealed, the telescopic pipe comprises two layers which are arranged inside and outside, the telescopic pipe, the upper plate, the telescopic pipe and the lower plate are well sealed, and an air cavity is formed between the inner layer and the outer layer of the telescopic pipe in a surrounding manner; an upper communication hole is formed in the lower plate, a lower communication hole communicated with the upper communication hole is formed in the top of the upper cover, an air exchanging pipe communicated with the air cavity is fixed at the top of the upper plate, a pair of baffles which are arranged up and down are detachably arranged on the inner side of the air exchanging pipe, a sealing ring fixedly connected with the inner wall of the air exchanging pipe is arranged between the two baffles, a sealing plug is arranged between the two baffles, a sealing spring is arranged between the sealing plug and the baffles, and the sealing plug is tightly contacted with the inner wall of the sealing ring under the action of the two sealing springs; an upper avoidance hole is formed in the center of the upper plate, a lower avoidance hole is formed in the center of the lower plate, and the round hole, the upper avoidance hole and the lower avoidance hole cooperate to clamp stems of plants;
the outer wall of the upper cover is fixedly provided with a guide pipe, the guide pipe is provided with a left conveying pipe and a right conveying pipe, and an adjusting unit for adjusting the opening and closing states and opening sizes of the left conveying pipe and the right conveying pipe is arranged in the guide pipe.
Further, a pair of slots are formed in the inner wall of the round hole, clamping plates are movably arranged in the slots, clamping springs are arranged between the clamping plates and the inner wall of the slots, and the two clamping plates are contacted and enclose a clamping hole under the action of the clamping springs.
Further, the top of upper cover is equipped with inside and outside first inner ring and the first outer loop that sets up, is equipped with inside and outside second inner ring and the second outer loop that sets up in the bottom of hypoplastron, second outer loop and first outer loop threaded connection, second inner ring and first inner ring threaded connection, and first inner ring, second inner ring, first outer loop and second outer loop set gradually from inside to outside.
Further, a soft rod is fixed at the top of the supporting rod, and the illumination unit comprises a lamp holder fixed on the soft rod, a regular octagon-shaped lamp box rotatably mounted on the lamp holder, and optical filters arranged on the side surfaces of the lamp box, wherein the optical filters on the side surfaces have different light transmittance.
Further, the lamp holder is of an inverted U-shaped structure, a left shell is fixed on the left side of the lamp holder, a sliding plate is slidably arranged in the left shell, a supporting pin is fixed on the sliding plate, an ejection spring is arranged between the sliding plate and the inner wall of the left shell, a knob arranged outside the left shell is fixed at the left end of the supporting pin, a square supporting block is fixed at the right end of the supporting pin, and the supporting block is arranged on the inner side of the lamp holder under the action of the ejection spring; a right shell is fixed on the right side of the lamp holder, a sliding plate is also slidably arranged in the right shell, a driving pin is fixed on the sliding plate, an ejection spring is also arranged between the sliding plate and the inner wall of the right shell, a square driving block is fixed at the left end of the driving pin, a knob arranged outside the right shell is fixed at the right end of the driving pin, and the driving block is arranged at the inner side of the lamp holder under the action of the ejection spring; the lamp box comprises a lamp box body, a lamp, a support block, a bearing, a positioning plate, a driving hole, a positioning plate, a positioning hole and a driving block, wherein the bearing is fixed at the left end of the lamp box body, the positioning plate is fixed on the inner ring of the bearing, the positioning plate is provided with a positioning hole matched with the support block, and the driving hole is arranged at the right end of the lamp box body and is in plug-in fit with the driving block.
Further, a square guide block is fixed on the outer wall of the supporting pin, a left track plate is fixed in the left shell, and the guide block is in sliding fit with a guide hole in the left track plate.
Further, a track seat is fixed on the baffle, guide rods are respectively fixed at the top and the bottom of the sealing plug, the guide rods are in sliding connection with the corresponding track seats, and a sealing spring is arranged between the track seat and the sealing plug.
Further, the adjusting unit comprises a sealing block, an adjusting box, a screw rod, a driven bevel gear, a driving bevel gear, a worm and an adjusting wheel, wherein the sealing block is arranged in the guide pipe in a sliding manner, the adjusting box is fixedly connected with the guide pipe, the screw rod is in threaded connection with the sealing block and is in rotary connection with the guide pipe, the driven bevel gear is fixed on the screw rod, the driving bevel gear is meshed with the driven bevel gear and is rotatably arranged in the adjusting box, the worm wheel is coaxially arranged with the driving bevel gear, the worm is meshed with the worm wheel and is rotatably connected with the adjusting box, and the adjusting wheel is fixed on the worm; the guide pipe is internally provided with a chute which is in sliding connection with the sealing block, the lower parts of the inner cavities of the left conveying pipe and the right conveying pipe are square holes, the top of the sealing block is provided with a vertical groove, the bottom of the sealing block is provided with a transverse groove communicated with the vertical groove, and the size of the vertical groove is the same as the size of the square hole.
Further, the sealing ring is a circular rubber piece, and the fillets at the two ends of the inner cavity of the sealing ring are in transition.
The application method of the clone plant functional character observation device is characterized by comprising the following steps:
(1) Clamping the cloned plants in an upper cover, and then adding culture solution into a culture bottle;
(2) Turning on an illuminating lamp according to the requirement, illuminating the cloned plants, and adjusting the illumination intensity according to the requirement;
(3) Plant height of the cloned plants was measured by a measuring scale.
The beneficial effects of the invention are as follows: the device for observing the functional properties of the cloned plants and the operation method can realize the cultivation of the cloned plants; the adjusting unit is arranged, and the needed mixed liquid can be added into the culture flask according to the needs; the illumination unit is arranged to provide illumination of a desired intensity for the growth of the cloned plants. When the gas in the culture bottle needs to be replaced, the ventilation box is pressed downwards and then lifted upwards. The device can conveniently realize the cultivation and the character observation of the cloned plants.
Drawings
FIG. 1 is a three-dimensional schematic of the present invention;
FIG. 2 is a front view of the present invention;
FIG. 3 is a schematic top view of the present invention;
FIG. 4 is a three-dimensional schematic view of the upper cover;
FIG. 5 is a top view of the upper cover;
FIG. 6 is a cross-sectional view of the upper cover;
FIG. 7 is a schematic view of an installation of a lighting unit;
fig. 8 is a schematic cross-sectional view of a lamp holder;
FIG. 9 is a three-dimensional schematic view of a light cartridge;
fig. 10 is a right side view of the lamp cartridge;
FIG. 11 is one of three-dimensional schematic views of a ventilation unit;
FIG. 12 is a second three-dimensional schematic of a ventilation unit;
FIG. 13 is a schematic cross-sectional view of the ventilation box;
FIG. 14 is a schematic view showing the internal structure of the ventilation tube;
FIG. 15 is a schematic view of the installation of a catheter with a flask;
FIG. 16 is a schematic view of an adjustment unit;
FIG. 17 is a three-dimensional schematic of a seal block;
FIG. 18 is a schematic cross-sectional view of a seal ring;
FIG. 19 is a schematic view of the seal block translating a distance B;
FIG. 20 is a three-dimensional schematic view of a sealing plug;
in the figure: the upper cover, the fixing block, the 111 threaded holes, the 12 connecting pipes, the 13 first inner ring, the 14 first outer ring, the 15 round holes, the 151 slot, the 16 clamping plates, the 17 clamping springs, the 18 clamping holes and the 19 lower communication holes are arranged on the upper cover, the 11 fixing block, the 111 threaded holes, the 12 connecting pipes, the 13 first inner ring, the 14 first outer ring, the 15 round holes, the 151 slot, the 16 clamping plates and the 17 clamping springs;
2 supporting rods, 21 soft rods, 22 lamp holders, 221 left through holes, 222 right through holes, 23 left shells, 231 left track plates, 232 sliding plates, 233 supporting pins, 234 guide blocks, 235 supporting blocks, 24 right shells, 241 right track plates, 242 driving pins, 243 driving blocks, 25 knobs and 26 ejection springs;
3 lamp box, 31 filter, 32 bearing, 33 locating plate, 331 locating hole, 34 driving hole;
4 lower plate, 41 upper plate, 411 upper avoidance hole, 42 telescopic tube, 43 second inner ring, 44 second outer ring, 45 lower avoidance hole, 46 upper communication hole, 47 air cavity;
5 ventilation pipes, 51 baffle plates, 511 ventilation holes, 52 vertical rods, 53 sealing rings, 531 round corner transition, 54 sealing plugs, 541 guide rods, 55 track seats and 56 sealing springs;
6, culturing the bottle;
7 guide pipes, 71 mounting grooves, 72 left conveying pipes, 73 right conveying pipes, 74 sliding grooves, 75 sealing blocks, 751 vertical grooves, 752 horizontal grooves, 76 lead screws, 8 adjusting boxes, 81 worms, 82 adjusting wheels, 83 worm gears, 84 driving bevel gears, 85 driven bevel gears, 86 scale plates, 87 pointers and 88 proportional scale marks;
9 measuring tape.
Detailed Description
As shown in fig. 1 to 20, the cloned plant functional trait observing device of the present invention mainly comprises an upper cover 1, a support bar 2, an illumination unit, a ventilation unit, a culture flask 6 and an adjusting unit, and the present invention is described in detail with reference to the accompanying drawings.
As shown in fig. 4 to 6, the upper cover 1 has a cylindrical hollow structure with an open bottom, a fixing block 11 and a connection pipe 12 are fixed to a circular outer wall of the upper cover, and a screw hole 111 is provided on an upper surface of the fixing block. The upper surface of the upper cover is provided with a circular ring-shaped first inner ring 13 and a circular ring-shaped first outer ring 14 which are arranged inside and outside, the center of the upper cover is provided with a round hole 15, the inner wall of the round hole is provided with a pair of slots 151, a semicircular clamping plate 16 is movably arranged in each slot, a clamping spring 17 is arranged between the clamping plate and the inner wall of each slot, the two clamping plates are contacted and enclosed into a circular clamping hole 18 under the action of the clamping spring, and a lower communication hole 19 is arranged between the first inner ring and the first outer ring. In use, the stem of the cloned plant is placed in the holding well.
As shown in fig. 1 and 2, a support rod 2 is arranged on a threaded hole of a fixing block in a threaded manner, as shown in fig. 7, a soft rod 21 is fixed at the top of the support rod, a gooseneck is fixed on the soft rod, a lamp holder 22 is fixed on the soft rod, as shown in fig. 8, the lamp holder is of an inverted U-shaped structure, a left through hole 221 is arranged on the left side of the lamp holder, a right through hole 222 is arranged on the right side of the lamp holder, a hollow left shell 23 corresponding to the left through hole is fixed on the left side of the lamp holder, a left track plate 231 is fixed in the inner cavity of the left shell, a sliding plate 232 is slidably arranged in the inner cavity of the left shell, a support pin 233 is fixed on the sliding plate, a square guide block 234 is fixed on the outer wall in the middle of the support pin, and the guide block is slidably connected with the guide hole on the left track plate, and the guide block is arranged so that the support pin can only move left and right relative to the left shell; a knob 25 is fixed to the left end of the support pin, and a square support block 235 is fixed to the right end of the support pin. An ejection spring 26 is arranged between the sliding plate and the inner wall of the left shell, and the supporting block passes through the left through hole and is arranged between the two ends of the lamp bracket under the action of the ejection spring. A hollow right housing 24 corresponding to the right through hole is fixed on the right side of the lamp holder, a right track plate 241 is fixed in the right housing, a sliding plate 232 is movably mounted in the right housing, and a driving pin 242 is fixed on the sliding plate and can move and rotate relative to the right track plate. An ejection spring 26 is arranged between the slide plate and the inner wall of the right shell, a driving block 243 is fixed at the left end of the driving pin, a knob 25 is fixed at the right end of the driving pin, and the driving block passes through the right through hole under the action of the ejection spring and is arranged between the two ends of the lamp bracket.
The lamp box 3 is placed between two ends of the lamp holder, and as shown in fig. 9, the lamp box is of a hollow structure in a shape of a regular octagon, and the eight sides of the lamp box are respectively provided with optical filters 31, and the light transmittance of each optical filter is different. The left end of the lamp box is provided with a bearing mounting hole, a bearing 32 is fixed in the bearing mounting hole, a positioning plate 33 is fixed on the inner ring of the bearing, a lighting lamp arranged in the lamp box is fixed on the positioning plate, and a square positioning hole 331 is arranged on the positioning plate. As shown in fig. 10, a square driving hole 34 is provided on the right end face of the lamp box, when the lamp box is mounted, the knob is pulled to enable the supporting block to be placed in the left through hole and the driving block to be placed in the right through hole, then the lamp box is placed in the lamp holder, and the knob is released to enable the supporting block to be placed in the positioning hole and the driving block to be placed in the driving hole. When the knob on the right side is rotated at this time, the lamp box can be driven to rotate. The lamp box is rotated, so that the optical filter with the required light transmittance faces downwards, and the illumination intensity of plants is adjusted. The lamp box, the light, the lighting fixture, the left casing, the right casing, the left track plate, the slide plate, the supporting pin, the supporting block, the spring, the supporting block, the right track plate, the driving pin and the driving block form an illumination unit.
The top of upper cover is equipped with the ventilation box, and as shown in fig. 11, 12, the ventilation box includes upper plate 41, flexible pipe 42 and the hypoplastron 4 that top-down set gradually, and flexible pipe's upper end links firmly and seals well with the upper plate, and flexible pipe's lower extreme links firmly and seals well with the hypoplastron. As shown in fig. 13, the telescopic tube comprises an inner layer and an outer layer, an air cavity 47 is enclosed between the inner layer and the outer layer of the telescopic tube, an upper avoiding hole 411 is arranged at the center of the upper plate, a lower avoiding hole 45 is arranged at the center of the lower plate, and the upper and lower avoiding holes are matched with the clamping holes on the upper cover to clamp the stem of the cloned plant. A second inner ring 43 and a second outer ring 44 are provided at the bottom of the lower plate, the annular region between the second inner ring and the second outer ring corresponds to the air chamber, and an upper communication hole 46 is provided between the second inner ring and the second outer ring, and the upper communication hole communicates with the air chamber. An air exchanging pipe 5 is fixed at the top of the upper cover and is communicated with the air cavity.
During installation, the second outer ring is in threaded connection with the first outer ring, and the second inner ring is in threaded connection with the first inner ring. At this time, the ventilation pipe is communicated with the inner cavity of the upper cover through the air cavity, the upper communication hole and the lower communication hole, and the first inner ring, the second inner ring, the first outer ring and the second outer ring are sequentially arranged from inside to outside.
As shown in fig. 14, a pair of baffles 51 are provided in the inner cavity of the ventilation pipe, and the baffles are screwed with the ventilation pipe and are well sealed. As shown in fig. 3, a plurality of vent holes 511 uniformly arranged along the same circumference are arranged on the baffle plates, a circular sealing ring 53 is fixed on the inner wall of the ventilation pipe between the two baffle plates, as shown in fig. 18, both ends of the inner cavity of the sealing ring are in rounded transition 531, and the sealing ring is a rubber piece. A sealing plug 54 is arranged on the inner side of the sealing ring and is of a hollow structure so as to reduce weight; as shown in fig. 20, the sealing plug comprises three coaxially arranged cylinders. Guide rods 541 are fixed at the top and the bottom of the sealing plug, a track seat 55 is fixed at the bottom of the upper baffle plate, a track seat 55 is also fixed at the top of the lower baffle plate, the guide rods are in one-to-one correspondence with the track seats, and the guide rods are arranged in the inner cavities of the track seats. A sealing spring 56 is arranged between the sealing plug and the track seat, and the stiffness coefficient and the length of the two sealing springs are the same. Under the effect of the sealing spring, the sealing plug is tightly contacted with the inner wall of the sealing ring, so that the sealing of the ventilation pipe is realized. During installation, the upper surface of the upper baffle plate is coplanar with the upper surface of the ventilation pipe, the lower surface of the lower baffle plate is flush with the lower surface of the ventilation pipe, and the deformation amount of the sealing spring is zero. A vertical rod 52 of a regular hexagonal prism shape is fixed on the top of the upper stop rod so as to facilitate the assembly and disassembly of the upper baffle plate. One end of the sealing spring is fixedly connected with the sealing plug, and the other end of the sealing spring is contacted with the track seat. The ventilation unit is formed by the ventilation box, the ventilation pipe, the sealing plug, the sealing ring, the sealing spring, the track seat, the guide rod and the baffle plate.
The upper cover is arranged on the bottle mouth of the culture bottle 6 in a threaded manner, the culture bottle is a transparent glass bottle, when the sealing plug moves upwards or downwards until the sealing plug moves out of the sealing ring, the whole ventilation pipe is communicated up and down, gas outside the ventilation pipe can enter the culture bottle through the air cavity, the upper communication hole and the lower communication hole, and the gas inside the culture bottle can be discharged through the lower communication hole, the upper communication hole, the air cavity and the ventilation pipe.
When the air-exchanging box is pressed downwards, the cavity pressure formed by the culture bottle, the upper cover and the air-exchanging box is increased, so that the sealing plug is pushed to move upwards until the sealing plug is separated from the sealing ring, and at the moment, the air in the culture bottle is discharged through the upper cover, the air-exchanging box and the air-exchanging pipe; when the air-exchanging box is lifted upwards, the cavity pressure formed by the culture bottle, the upper cover and the air-exchanging box is reduced, and then the sealing plug is pushed to move downwards until the sealing plug is separated from the sealing ring, and at the moment, external air enters the culture bottle through the air-exchanging pipe, the air-exchanging box and the upper cover. When the air exchanging device is used, the baffle above the air exchanging device can be rotated to push the sealing plug to move downwards, so that the sealing plug is separated from the sealing ring, the air exchanging pipe is opened at the moment, and the culture bottle can exchange air with the outside automatically.
As shown in fig. 15, a pipe 7 is connected to the connecting pipe upper pipe, and as shown in fig. 16, a mounting groove 71 is provided at the upper part of the pipe, a left conveying pipe 72 and a right conveying pipe 73 provided in the left and right are fixedly mounted or screw-mounted in the mounting groove, and a square chute 74 is provided in the pipe, and the chute communicates with the left conveying pipe and the chute communicates with the right conveying pipe. A sealing block 75 is movably arranged in the sliding groove, the width of the sealing block is the same as that of the sliding groove, the height of the sealing block is the same as that of the sliding groove, and the sealing block is in close contact with the inner wall of the sliding groove. As shown in fig. 17, a square horizontal groove 752 is provided at the bottom of the sealing block, and a vertical groove 751 is provided at the top of the sealing block, the vertical groove communicating with the horizontal groove. The right end of the guide pipe is sealed, a screw rod 76 is rotatably arranged at the right end of the guide pipe, and the screw rod is in threaded connection with the right end of the sealing block. An adjusting box 8 is fixed at the right end of the guide pipe, the right end of the screw rod extends into the adjusting box, a driven bevel gear 85 is fixed at the right end of the screw rod, a worm gear 83 and a driving bevel gear 84 which are coaxially arranged are rotatably arranged in the adjusting box, the driving bevel gear is meshed with the driven bevel gear, a worm 81 is rotatably arranged on the adjusting box, and the worm is meshed with the worm gear. The worm shaft of the worm is fixed with an adjusting wheel 82 arranged outside the adjusting box, the worm shaft is fixed with a pointer 87 arranged outside the adjusting box, the top of the adjusting box is fixed with a scale plate 86, and as shown in fig. 3, the scale plate is provided with scale marks 88 uniformly arranged along the circumferential direction. When in a natural state, the sealing block is arranged at the leftmost end of the chute, at the moment, the sealing block plugs the bottom of the left conveying pipe, and the vertical chute is communicated with the right conveying pipe. The transmission ratio between the driving bevel gear and the driven bevel gear is large, so that when the adjusting wheel rotates by a small angle, the screw rod can rotate for a plurality of circles. When the adjusting wheel rotates for one circle, the screw rod rotates for N circles, and the sealing block moves to the maximum stroke. The lower parts of the inner cavities of the left conveying pipe and the right conveying pipe are square holes, the size of the square holes is the same as the cross section size of the vertical groove, and the length of the square holes is L. The adjusting box, the adjusting wheel, the worm wheel, the scale plate, the driving bevel gear, the driven bevel gear and the screw rod form an adjusting unit.
When the adjusting wheel is rotated, the worm can drive the worm wheel to rotate so as to drive the driving bevel gear to rotate, and then drive the driven bevel gear to rotate so as to drive the screw rod to rotate, and then drive the sealing block to move left and right; when the regulating wheel is rotated to align the pointer with a certain scale A (A is more than or equal to 0 and less than or equal to 100) on the scale mark, as shown in FIG. 19, the sealing block horizontally moves by a distance B relative to the guide pipe, at the moment, the bottom of the inner cavity of the left conveying pipe is opened, the bottom of the inner cavity of the right conveying pipe is blocked, and the bottom of the inner cavity of the right conveying pipe is opened (L-B)/L. B is 0 or more and L or less. When in use, the culture solution can be added through the left conveying pipe, and the water can be added through the right conveying pipe.
When only culture solution is needed to be added into the culture bottle, the regulating wheel can be rotated to enable the pointer to be aligned with 100% scale marks, the sealing block is arranged at the rightmost end of the chute, the left conveying pipe is completely opened, and the right conveying pipe is completely closed; when only water is needed to be added into the culture flask, the regulating wheel can be rotated to enable the pointer to be aligned with 0% scale marks, the sealing block is arranged at the leftmost end of the chute, the right conveying pipe is completely opened, and the left conveying pipe is completely closed; when the culture solution in the mixed solution added into the culture flask accounts for A%, the regulating wheel is rotated to enable the pointer to be aligned with the A% scale mark, at the moment, the sealing block moves rightwards by a distance B, the bottom of the left conveying pipe is opened by B/L (A=B/L), and the bottom of the right conveying pipe is opened by (L-B)/L.
In order to realize the measurement of the stem length of the cloned plant in the culture flask, a measuring scale 9 which is vertically arranged is fixed in the culture flask, and the measurement of the stem length of the cloned plant is realized through the measuring scale.
The application method of the clone plant functional character observation device comprises the following steps:
(3) Clamping the cloned plants in an upper cover, and then adding culture solution into a culture bottle;
(4) Turning on an illuminating lamp according to the requirement, illuminating the cloned plants, and adjusting the illumination intensity according to the requirement;
(5) Plant height of the cloned plants was measured by a measuring scale.
Claims (7)
1. The clone plant functional character observation device is characterized by comprising an upper cover, a culture bottle, a support rod, an illumination unit, a ventilation unit, a guide pipe, an adjusting unit and a measuring scale, wherein the upper cover is arranged on the bottle mouth of the culture bottle in a threaded manner, a round hole for placing plant stems is formed in the center of the upper cover, the measuring scale which is vertically placed is arranged in the culture bottle, the support rod is detachably arranged on the side wall of the upper cover, and the illumination unit with adjustable illumination intensity is arranged on the support rod; the top of the supporting rod is fixedly provided with a soft rod, the illumination unit comprises a lamp holder fixed on the soft rod, a regular octagon-shaped lamp box rotatably arranged on the lamp holder, and optical filters arranged on the side surfaces of the lamp box, and the optical filters on the side surfaces have different light transmission properties; the lamp bracket is of an inverted U-shaped structure, a left shell is fixed on the left side of the lamp bracket, a sliding plate is slidably arranged in the left shell, a supporting pin is fixed on the sliding plate, an ejection spring is arranged between the sliding plate and the inner wall of the left shell, a knob arranged outside the left shell is fixed at the left end of the supporting pin, a square supporting block is fixed at the right end of the supporting pin, and the supporting block is arranged on the inner side of the lamp bracket under the action of the ejection spring; a right shell is fixed on the right side of the lamp holder, a sliding plate is also slidably arranged in the right shell, a driving pin is fixed on the sliding plate, an ejection spring is also arranged between the sliding plate and the inner wall of the right shell, a square driving block is fixed at the left end of the driving pin, a knob arranged outside the right shell is fixed at the right end of the driving pin, and the driving block is arranged at the inner side of the lamp holder under the action of the ejection spring; the left end of the lamp box is fixedly provided with a bearing, the inner ring of the bearing is fixedly provided with a positioning plate, the positioning plate is provided with a positioning hole matched with the supporting block, the right end of the lamp box is provided with a driving hole, and the driving hole is in plug-in fit with the driving block;
the top of the upper cover is provided with a ventilation unit, the ventilation unit comprises a ventilation box and a ventilation pipe, the ventilation box comprises an upper plate, a telescopic pipe and a lower plate which are sequentially arranged from top to bottom, the lower plate is in threaded connection with the top of the upper cover, the lower plate and the top of the upper cover are well sealed, the telescopic pipe comprises two layers which are arranged inside and outside, the telescopic pipe, the upper plate, the telescopic pipe and the lower plate are well sealed, and an air cavity is formed between the inner layer and the outer layer of the telescopic pipe in a surrounding manner; an upper communication hole is formed in the lower plate, a lower communication hole communicated with the upper communication hole is formed in the top of the upper cover, an air exchanging pipe communicated with the air cavity is fixed at the top of the upper plate, a pair of baffles which are arranged up and down are detachably arranged on the inner side of the air exchanging pipe, a sealing ring fixedly connected with the inner wall of the air exchanging pipe is arranged between the two baffles, a sealing plug is arranged between the two baffles, a sealing spring is arranged between the sealing plug and the baffles, and the sealing plug is tightly contacted with the inner wall of the sealing ring under the action of the two sealing springs; an upper avoidance hole is formed in the center of the upper plate, and a lower avoidance hole is formed in the center of the lower plate; the round hole, the upper avoidance hole and the lower avoidance hole which are positioned in the center of the upper cover are matched to clamp stems of plants;
a guide pipe is fixed on the outer wall of the upper cover, a left conveying pipe and a right conveying pipe are arranged on the guide pipe, and an adjusting unit for adjusting the opening and closing states and opening sizes of the left conveying pipe and the right conveying pipe is arranged in the guide pipe; the adjusting unit comprises a sealing block, an adjusting box, a screw rod, a driven bevel gear, a driving bevel gear, a worm and an adjusting wheel, wherein the sealing block is arranged in the guide pipe in a sliding manner, the adjusting box is fixedly connected with the guide pipe, the screw rod is in threaded connection with the sealing block and is in rotary connection with the guide pipe, the driven bevel gear is fixed on the screw rod, the driving bevel gear is meshed with the driven bevel gear and is rotatably arranged in the adjusting box, the worm gear is coaxially arranged with the driving bevel gear, the worm is meshed with the worm gear and is rotatably connected with the adjusting box, and the adjusting wheel is fixed on the worm; the guide pipe is internally provided with a chute which is in sliding connection with the sealing block, the lower parts of the inner cavities of the left conveying pipe and the right conveying pipe are square holes, the top of the sealing block is provided with a vertical groove, the bottom of the sealing block is provided with a transverse groove communicated with the vertical groove, and the size of the vertical groove is the same as the size of the square hole.
2. The observing device for the functional properties of the cloned plants according to claim 1, wherein a pair of slots are formed in the inner wall of the round hole in the center of the upper cover, clamping plates are movably arranged in the slots, clamping springs are arranged between the clamping plates and the inner wall of the slots, and the two clamping plates are contacted and enclose a clamping hole under the action of the clamping springs.
3. The observing device for the functional properties of the cloned plants according to claim 1, wherein a first inner ring and a first outer ring which are arranged inside and outside are arranged at the top of the upper cover, a second inner ring and a second outer ring which are arranged inside and outside are arranged at the bottom of the lower plate, the second outer ring is in threaded connection with the first outer ring, the second inner ring is in threaded connection with the first inner ring, and the first inner ring, the second inner ring, the first outer ring and the second outer ring are sequentially arranged from inside to outside.
4. The observing device for the functional properties of the cloned plants according to claim 1, wherein square guide blocks are fixed on the outer wall of the supporting pin, a left track plate is fixed in the left shell, and the guide blocks are in sliding fit with guide holes in the left track plate.
5. The observing device for the functional properties of the cloned plants according to claim 4, wherein a rail seat is fixed on the baffle plate, guide rods are respectively fixed at the top and the bottom of the sealing plug, the guide rods are in sliding connection with the corresponding rail seat, and a sealing spring is arranged between the rail seat and the sealing plug.
6. The observing device for the functional properties of the cloned plants according to claim 1, wherein the sealing ring is a circular rubber piece, and fillets at two ends of an inner cavity of the sealing ring are in transition.
7. The method of using a cloned plant functional trait observation apparatus according to any one of claims 1 to 6, comprising the steps of:
(1) Clamping the cloned plants in an upper cover, and then adding culture solution into a culture bottle;
(2) Turning on an illuminating lamp according to the requirement, illuminating the cloned plants, and adjusting the illumination intensity according to the requirement;
(3) Plant height of the cloned plants was measured by a measuring scale.
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Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR19990001509A (en) * | 1997-06-16 | 1999-01-15 | 이성규 | Plant Growing Apparatus |
| WO2000005942A1 (en) * | 1998-07-28 | 2000-02-10 | Institute Of Genetics, Chinese Academy Of Sciences | Culture container and process for producing potato microtubers by using the same |
| JP2001045897A (en) * | 1999-08-02 | 2001-02-20 | Free Kogyo Kk | Culturing bamboo grass and green field formation |
| CN2466900Y (en) * | 2001-03-21 | 2001-12-26 | 中国科学院遗传研究所 | Culture container |
| JP2003143986A (en) * | 2001-11-16 | 2003-05-20 | Mukoyama Orchids Co Ltd | Plant-growing container and method for growing plant |
| WO2005058022A1 (en) * | 2003-12-19 | 2005-06-30 | Yongtai Zhang | Combination-type plant sugarless tissue culture propagation device and method thereof |
| WO2014130362A1 (en) * | 2013-02-25 | 2014-08-28 | Heliae Development, Llc | Systems and methods for the continuous optimization of a microorganism culture profile |
| KR20160053748A (en) * | 2014-11-05 | 2016-05-13 | 윤병섭 | Fabricated Multi-Function Flowerpot Assembly and Flowerpot and strut with the Same |
| CN105594566A (en) * | 2014-09-30 | 2016-05-25 | 中国航天员科研训练中心 | Device and method for low-pressure plant gas exchange measurement |
| CN105875408A (en) * | 2016-04-13 | 2016-08-24 | 嘉兴职业技术学院 | Flower reproduction equipment and cultivating method |
| CN107980638A (en) * | 2017-12-29 | 2018-05-04 | 武汉艾德士生物科技有限公司 | A kind of energy-saving tissue culture case |
| CN207692643U (en) * | 2017-11-13 | 2018-08-07 | 北京建筑大学 | A kind of microphyte incubator |
| CN108605466A (en) * | 2018-04-23 | 2018-10-02 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | A kind of bathmic device of monitoring crop seeds main root and monitoring method |
| CN209201774U (en) * | 2018-10-24 | 2019-08-06 | 杭州师范大学 | Clone plant functional trait observes device |
-
2018
- 2018-10-24 CN CN201811244589.4A patent/CN109601376B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR19990001509A (en) * | 1997-06-16 | 1999-01-15 | 이성규 | Plant Growing Apparatus |
| WO2000005942A1 (en) * | 1998-07-28 | 2000-02-10 | Institute Of Genetics, Chinese Academy Of Sciences | Culture container and process for producing potato microtubers by using the same |
| JP2001045897A (en) * | 1999-08-02 | 2001-02-20 | Free Kogyo Kk | Culturing bamboo grass and green field formation |
| CN2466900Y (en) * | 2001-03-21 | 2001-12-26 | 中国科学院遗传研究所 | Culture container |
| JP2003143986A (en) * | 2001-11-16 | 2003-05-20 | Mukoyama Orchids Co Ltd | Plant-growing container and method for growing plant |
| WO2005058022A1 (en) * | 2003-12-19 | 2005-06-30 | Yongtai Zhang | Combination-type plant sugarless tissue culture propagation device and method thereof |
| WO2014130362A1 (en) * | 2013-02-25 | 2014-08-28 | Heliae Development, Llc | Systems and methods for the continuous optimization of a microorganism culture profile |
| CN105594566A (en) * | 2014-09-30 | 2016-05-25 | 中国航天员科研训练中心 | Device and method for low-pressure plant gas exchange measurement |
| KR20160053748A (en) * | 2014-11-05 | 2016-05-13 | 윤병섭 | Fabricated Multi-Function Flowerpot Assembly and Flowerpot and strut with the Same |
| CN105875408A (en) * | 2016-04-13 | 2016-08-24 | 嘉兴职业技术学院 | Flower reproduction equipment and cultivating method |
| CN207692643U (en) * | 2017-11-13 | 2018-08-07 | 北京建筑大学 | A kind of microphyte incubator |
| CN107980638A (en) * | 2017-12-29 | 2018-05-04 | 武汉艾德士生物科技有限公司 | A kind of energy-saving tissue culture case |
| CN108605466A (en) * | 2018-04-23 | 2018-10-02 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | A kind of bathmic device of monitoring crop seeds main root and monitoring method |
| CN209201774U (en) * | 2018-10-24 | 2019-08-06 | 杭州师范大学 | Clone plant functional trait observes device |
Non-Patent Citations (2)
| Title |
|---|
| Development of a heat transfer model for plant tissue culture vessels;Chen CC;《Biosystems Engineering》;第85卷(第01期);第66-77页 * |
| 平板式光生物反应器在饵料微藻培养中的应用;孙利芹、史磊、王长海;《烟台大学学报(自然科学与工程版)》;第20卷(第02期);第112-115页 * |
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