CN109601360B - Aquatic plant cloning character dynamic observation device and operation method - Google Patents

Aquatic plant cloning character dynamic observation device and operation method Download PDF

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Publication number
CN109601360B
CN109601360B CN201811242132.XA CN201811242132A CN109601360B CN 109601360 B CN109601360 B CN 109601360B CN 201811242132 A CN201811242132 A CN 201811242132A CN 109601360 B CN109601360 B CN 109601360B
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rod
measuring rod
base
assembly
guide pipe
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CN109601360A (en
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余华
舒王凯
杨俊�
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Hangzhou Normal University
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Hangzhou Normal University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01BMEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
    • G01B5/00Measuring arrangements characterised by the use of mechanical techniques
    • G01B5/02Measuring arrangements characterised by the use of mechanical techniques for measuring length, width or thickness
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The aquatic plant cloning character dynamic observation device and the operation method are used for facilitating the growth dynamic observation of the cloned plants. The device comprises a base, a support rod, a lamp post, an illumination assembly, a transition rod, an inner rod, an observation assembly, a guide pipe and a disinfection assembly, wherein the support rod and the transition rod are arranged on the base, the lamp post is movably arranged on the support rod, and the illumination assembly is arranged on the lamp post; an inner rod is movably arranged on the transition rod, a flexible damping sleeve is sleeved on the upper part of the inner rod, the upper part of the inner rod is arranged in the inner cavity of the transition rod, and the damping sleeve is tightly contacted with the inner wall of the transition rod; an observation assembly is detachably arranged at the lower part of the inner rod; the base is detachably provided with a guide pipe, a pair of left and right valves are arranged on the guide pipe, a culture solution barrel communicated with the guide pipe is arranged between the two valves, the guide pipe is provided with a boss, the boss is of a hollow structure with an open top, and the boss is provided with a disinfection assembly. The invention can conveniently realize the dynamic observation of the growth of the cloned plants.

Description

Aquatic plant cloning character dynamic observation device and operation method
Technical Field
The invention relates to the technical field of cloning plant growth observation, in particular to a dynamic observation device for cloning characters of aquatic plants and an operation method.
Background
The plant cloning character is one of important tissues, organs and main propagation modes of plants, and is important content for researching individual physiological adaptation strategy and stress response mechanism of the plants. However, current studies on cloning traits of aquatic plants generally observe and measure by collecting individual plants and tissues and organs, and the method damages the plants and is unfavorable for long-term dynamic observation. Therefore, a dynamic observation device for cloning characters of aquatic plants is urgently needed, and in-situ nondestructive observation and sustainable dynamic research on cloning characters of the aquatic plants in the plant culture process are realized.
Disclosure of Invention
The invention aims to provide a dynamic observation device and an operation method for cloning characters of aquatic plants, which are used for conveniently and dynamically observing growth of the cloned plants.
The technical scheme adopted for solving the technical problems is as follows: the aquatic plant cloning character dynamic observation device is characterized by comprising a base, a support rod, a lamp post, an illumination assembly, a transition rod, an inner rod, an observation assembly, a guide pipe and a disinfection assembly, wherein the base is of a hollow structure with an open bottom, a clamping hole is formed in the top of the base, the clamping hole comprises a semicircular hole and a square hole which are arranged left and right and are communicated, a slot is formed in the inner wall of the clamping hole, a limiting plate is slidably arranged in the slot, a stem hole which is circular is formed between the limiting plate and the semicircular hole in a surrounding mode, and a clamping spring which is positioned in the slot is arranged between the limiting plate and the base;
the base is detachably provided with a support rod and a transition rod which are arranged up and down, the support rod is movably provided with a lamp post, the lamp post is provided with an illumination assembly with adjustable illumination intensity, and a locking bolt is arranged between the lamp post and the support rod;
an inner rod is movably arranged on the transition rod, a flexible damping sleeve is sleeved on the upper part of the inner rod, the upper part of the inner rod is arranged in the inner cavity of the transition rod, and the damping sleeve is tightly contacted with the inner wall of the transition rod; an observation assembly is detachably arranged at the lower part of the inner rod;
the base is detachably provided with a guide pipe, a pair of left and right valves are arranged on the guide pipe, a culture solution barrel communicated with the guide pipe is arranged between the two valves, the guide pipe is provided with a boss, the boss is of a hollow structure with an open top, and the boss is provided with a disinfection assembly.
Further, a fixed block is fixed in the inner cavity of the base, the supporting rod is in threaded connection with the upper part of the fixed block, and the transition rod is in threaded connection with the lower part of the fixed block.
Further, the observation assembly comprises a main measuring rod, an auxiliary measuring rod movably arranged on the main measuring rod, a transverse measuring rod vertically arranged on the auxiliary measuring rod, an extension rod movably arranged on the transverse measuring rod, a cross arm arranged on the auxiliary measuring rod and a magnifying glass movably arranged on the cross arm.
Further, a flexible damping sleeve is sleeved on the outer wall of the upper part of the auxiliary measuring rod, a supporting lug is fixed on the transverse measuring rod, the supporting lug is arranged on the auxiliary measuring rod in a sliding mode, and a jackscrew is arranged between the supporting lug and the auxiliary measuring rod.
Further, be equipped with the locking unit between main measuring stick and interior pole, the locking unit is including fixing the lock of interior pole bottom, fix baffle in the lock inner chamber, slide the setting in the lock inner chamber, fix the locking round pin on the slide, set up the spring between slide and lock inner wall, the baffle is divided into front and back two parts with the inner chamber of lock, the effect end of locking round pin stretches into the rear portion of lock inner chamber under the effect of spring, be equipped with at the top of main measuring stick with locking round pin complex locking hole.
Further, the disinfection assembly includes disinfection lamp box, fix the bottom plate on the disinfection lamp box, be located a plurality of floating plates of bottom plate top, fix the disinfection lamp on the floating plate, set up the conductive spring between floating plate and bottom plate, set up the negative pole wire between disinfection lamp negative pole and battery, the activity sets up the switch at disinfection lamp box top, set up the conducting block in the switch bottom, set up the anodal wire between conducting block and battery positive pole, the height that the anodal place of disinfection lamp is greater than the lower surface place of conducting block under the effect of conductive spring in natural state, the battery sets up on the base.
Further, the bottom of the disinfection lamp box is provided with a plugboard which is matched with the boss in a plug-in manner.
Further, the left end and the bracing piece of first grip block link firmly, are equipped with the through-hole at the right-hand member of first grip block, rotate in the through-hole and install the rotatory ring of C shape, be equipped with the passageway on first grip block, the one end of passageway runs through the rear end of first grip block, the other end and the through-hole intercommunication of passageway.
The operation method of the aquatic plant cloning character dynamic observation device is characterized by comprising the following steps:
(1) The method comprises the steps of installing a supporting rod and a transition rod on a base, installing an observation assembly on an inner rod on the transition rod, installing a guide pipe on the base, and installing a disinfection assembly on a boss on the guide pipe;
(2) Placing the cloned plant in a culture container for culture, and placing stems of the cloned plant in a clamping plate;
(3) According to the requirement of the cloned plants on illumination intensity, rotating the lamp box to enable the corresponding light-transmitting optical filters to face downwards;
(4) According to the requirement of cultivating cloned plant, selecting whether to add tap water into the cultivation container; if tap water is needed to be added into the culture container, the clamping ring is connected with the water pipe, the two valves are opened, and tap water and culture solution are added into the culture container; if only the culture solution is needed to be added into the culture container, the right valve is closed, the left valve is opened, and the culture solution in the culture solution barrel enters the culture container; if only tap water is needed to be added into the culture container, closing a valve on the culture solution barrel and opening left and right valves, wherein tap water enters the culture container at the moment;
(5) In the process of cultivating the cloned plant, the length of the stem of the cloned plant is measured by a main measuring rod and an auxiliary measuring rod, and the outer diameter size of the stem of the plant or the root system width of the plant is measured by a transverse measuring rod and an extension rod; the appearance of the stem and root of the cloned plant was observed by a magnifying glass.
The beneficial effects of the invention are as follows: the aquatic plant cloning character dynamic observation device and the operation method provided by the invention can conveniently realize the cultivation of the cloned plants; in the culture process, the illumination intensity can be adjusted according to the requirement of the cloned plants on the illumination intensity; according to the demands of the cloned plants on the culture solution and tap water, the culture solution or the mixed solution of the two can be added into the culture container; the arrangement of the observation assembly can also measure and observe the stems and roots of plants in real time.
Drawings
FIG. 1 is a schematic illustration of the present invention;
FIG. 2 is a schematic cross-sectional view of a base;
FIG. 3 is a schematic top view of the base;
FIG. 4 is a schematic view of a support bar;
FIG. 5 is a schematic cross-sectional view of the support bar;
FIG. 6 is a three-dimensional schematic of an illumination assembly;
FIG. 7 is a schematic front view of an illumination assembly;
FIG. 8 is a partial view in the direction A of FIG. 4;
FIG. 9 is a schematic illustration of the placement of stems of plants in a holding plate;
FIG. 10 is a view in the B-direction of FIG. 7;
FIG. 11 is a schematic view of the installation of the right support shaft;
FIG. 12 is a schematic view of a transition rod;
FIG. 13 is a schematic view of an inner rod;
FIG. 14 is a schematic view of a locking unit;
FIG. 15 is a schematic view of the installation of a magnifying glass with a cross arm;
FIG. 16 is a schematic view of a viewing assembly;
FIG. 17 is a schematic view of a sterilization assembly;
FIG. 18 is a schematic bottom view of the sterilizing light cartridge;
FIG. 19 is a schematic top view of a sterilizing light cartridge;
FIG. 20 is a schematic diagram of the switch and the positions of the sterilizing lamps;
FIG. 21 is a schematic diagram of a switch;
FIG. 22 is a three-dimensional schematic of a light box;
FIG. 23 is an axial schematic view of a damping sleeve;
in the figure: 1 a base, 11 a fixing block, 12 a round hole, 13 a slot, 14 a limiting plate, 15 a clamping spring, 16 a stem hole, 17 a connecting pipe and 18 a clamping hole;
the device comprises a support rod 2, a lamp post 21, a fixing ring 22, a locking bolt 23, a first clamping plate 24, a through hole 240, a rotary ring 241, a 242 notch, a 243 channel 244 conical opening 25, a lamp holder 251, a shaft hole 251 and a plant stem 26;
the lamp box 3, the mounting hole 30, the square hole 31, the optical filter 32, the inner side plate 33, the outer side plate 34, the bearing 35, the left support shaft 36, the fixed cylinder 37, the right support shaft 38 and the ejection spring 39;
the transition rod 4, the inner rod 41, the 411 damping sleeve 412 damping ring 42 second clamping plate 43 locker 431 baffle plate 432 slide plate 433 locking pin 434 locking spring 435 pull ring;
the device comprises a main measuring rod 5, an auxiliary measuring rod 51, a length scale line 52, a transverse measuring rod 53, an extension rod 54, a support lug 55, a jackscrew 56 and a locking hole 57;
6 cross arm, 61 magnifier, 62 handle;
7 guide pipes, 71 valves, 72 bosses, 73 culture solution barrels and 74 clamping rings;
8 sterilizing lamp boxes, 81 plugboards, 82 sterilizing lamps, 821 anodes, 83 switches, 831 sliders, 84 conductive blocks, 841 arc surfaces, 85 anode wires, 86 bottom boards, 87 floating boards, 88 conductive springs and 89 cathode wires.
Detailed Description
As shown in fig. 1 to 23, the present invention mainly comprises a base 1, a support rod 2, a lighting assembly, a transition rod 4, a viewing assembly and a sterilizing assembly, and is described in detail with reference to the accompanying drawings.
As shown in fig. 2 and 3, the base 1 has a cylindrical structure with an open bottom, a fixed block 11 is fixed in an inner cavity of the base, a threaded hole is formed in the fixed block, and a round hole corresponding to the threaded hole is formed in the base. The base is provided with a clamping hole 18, the clamping hole comprises a semicircular hole on the left side and a square hole on the right side, a slot 13 is formed in the right inner wall of the clamping hole, a limiting plate 14 is movably installed in the slot, a clamping spring 15 is arranged between the limiting plate and the base, and a whole circular stem hole 16 is formed by the limiting plate and the inner wall of the clamping hole under the action of the clamping spring. The outer wall of the base is provided with a connecting pipe 17, and the base is arranged on a culture container for cloning plants.
As shown in fig. 4, a supporting rod 2 is arranged on the fixed block in a threaded manner, the supporting rod is of a hollow structure, as shown in fig. 5, a lamp post 21 is movably arranged on the upper part of the supporting rod, a fixed ring 22 is fixed on the outer wall of the upper part of the supporting rod, and a locking bolt 23 is arranged on the fixed ring; after the locking bolt is tightly propped against the lamp post, the locking fixation of the lamp post and the supporting rod can be realized. The outer wall of the support rod is fixed with a first clamping plate 24, as shown in fig. 8, the left end of the clamping plate is fixed on the support rod, the right end of the clamping plate is provided with a through hole 240, a rotating ring 241 is rotatably installed in the through hole, a notch 242 is arranged on the rotating ring, and the notch enables the rotating ring to be in a C-shaped structure. An L-shaped channel 243 is provided on the rotating ring, one end of the channel penetrates through the rear sidewall of the first clamping plate, and the other end of the channel is communicated with the through hole. One end of the channel extending through the rear sidewall of the first clamping plate is provided with a tapered opening 244 to facilitate the entry of plant stems into the channel. In use, as shown in fig. 9, the plant stem 26 is placed in the passageway and the swivel ring is rotated so that the gap communicates with the passageway such that after the plant stem enters the swivel ring through the gap, the swivel ring is rotated to trap the plant stem in the through hole. The upper part of the lamp post is provided with an inverted U-shaped lamp holder 25, the inner wall of the lamp holder is provided with a pair of shaft holes 251, and the lamp holder is provided with an illumination assembly.
As shown in fig. 6, 7 and 22, the illumination assembly comprises a lamp box 3, a light filter 32, a side plate 33, an outer plate 34, a bearing 35, a left support shaft 36, a fixed cylinder 37, a right support shaft 38 and an ejection spring 39, wherein the lamp box is of a hollow structure in the shape of a regular hexagonal prism, square holes 31 are respectively arranged on six sides of the lamp box, the light filter 32 is arranged in the square holes, the light transmittance of the six light filters is different, mounting holes 30 are respectively arranged at two ends of the lamp box, a circular outer plate 34 is fixed in the mounting hole at the left end, an inner plate 33 is arranged at the inner side of the outer plate, a bearing 35 is arranged between the inner plate and the outer plate, a left support shaft 36 arranged outside the lamp box is fixed on the inner plate, an illuminating lamp arranged at the inner side of the lamp box is fixed on the inner plate, an electric wire for connecting the illuminating lamp is led out from an inner cavity of the left support shaft to be electrically connected with a storage battery, and the storage battery is arranged on a base. As shown in fig. 10, a bearing is fixed in a mounting hole at the right end, a fixing cylinder 37 is fixed on an inner ring of the bearing, the fixing cylinder is of a hollow structure with one end open, a right support shaft 38 is movably mounted in an inner cavity of the fixing cylinder, an ejection spring is arranged between the right support shaft and the fixing cylinder, and most of the right support shaft is arranged outside the fixing cylinder under the action of the ejection spring.
When the lamp box is installed, the left support shaft is placed in the shaft hole on the left side, then the right end of the lamp box moves into the lamp holder, and the ejection spring is compressed after the right support shaft is contacted with the lamp holder, so that the right support shaft moves towards the inner side of the fixed cylinder until the right support shaft is aligned with the shaft hole on the right side, and the right support shaft enters the shaft hole on the right side under the action of the ejection spring. The left support shaft is provided with a flat key, and a key slot matched with the flat key is arranged in the left shaft hole so as to realize the relative fixation of the left support shaft and the lamp bracket. According to the requirement of the cloned plants on illumination intensity, the lamp box is rotated so that the corresponding optical filters face to the right lower side.
The transition rod 4 arranged below the base is arranged on the fixed block in a threaded manner, as shown in fig. 12, the transition rod is a hollow rod, an inner rod 41 is movably arranged in the inner cavity of the transition rod, as shown in fig. 13, a tubular damping sleeve 411 is arranged on the outer wall of the upper part of the inner rod, the damping sleeve is a rubber piece, and as shown in fig. 23, a circular damping ring 412 is arranged on the outer wall of the damping sleeve. A second clamping plate 42 is fixed on the outer wall of the inner rod, and the structural shape of the second clamping plate is identical to that of the first clamping plate.
The main measuring rod 5 is detachably mounted at the bottom of the inner rod, and as shown in fig. 16, a locking hole 57 is provided at the upper part of the main measuring rod, and a locking unit is provided between the main measuring rod and the inner rod.
As shown in fig. 14, the locking unit includes a locker 43, a baffle 431, a sliding plate 432, a locking pin 433, a spring 434 and a pull ring 435, the locker is of a cylindrical hollow structure, the baffle 431 is fixed in an inner cavity of the locker, the baffle divides the inner cavity of the locker into a front part and a rear part, the sliding plate 432 is arranged between the baffle and an inner wall of the locker, the locking pin 433 is fixed on the sliding plate, the locking pin is a square rod, the spring 434 is arranged between the sliding plate and a front wall of the locker, the sliding plate is in contact with the baffle under the action of the spring, and one end of the locking pin penetrates through the baffle and is arranged at the rear part of the inner cavity of the locker. In order to facilitate the pulling out of the locking pin from the rear part of the inner cavity of the locking device, a pull ring 435 arranged outside the locking device is fixed on the locking pin, and an avoidance hole for allowing the top of the main measuring rod to extend into the rear part of the inner cavity of the locking device is arranged on the outer wall of the locking device.
When the top of the main measuring rod passes through the avoiding hole and stretches into the rear part of the inner cavity of the locker and the locking pin passes through the locking hole, the main measuring rod and the inner rod are fixed together through the locking unit.
As shown in fig. 16, a sub-measuring rod 51 is movably mounted on the main measuring rod, and the sub-measuring rod is movable with respect to the main measuring rod to adjust the measuring length. The upper part of the auxiliary measuring rod is arranged in the inner cavity of the main measuring rod, and a damping sleeve is also arranged on the outer wall of the auxiliary measuring rod, so that the main measuring rod and the auxiliary measuring rod can not move freely. The auxiliary measuring rod is sleeved with a supporting lug 55, a jackscrew 56 is arranged on the supporting lug, and the supporting lug and the auxiliary measuring rod are relatively fixed under the action of the jackscrew. A transverse measuring rod 53 is fixed on the support lugs, an extension rod 54 is movably arranged on the transverse measuring rod, and the extension rod can move relative to the measuring rod so as to adjust the measuring range. Length scale lines 52 are arranged on the main measuring rod, the auxiliary measuring rod, the transverse measuring rod and the extension rod.
A cross arm 6 is fixed to the auxiliary measuring rod, and as shown in fig. 15, a magnifying glass 61 is provided on the cross arm, and a handle 62 of the magnifying glass is movably provided in the cross arm. When the handle is moved along the length direction of the cross arm, the position of the magnifying glass can be adjusted, so that the observation of plant stems or roots is facilitated.
The main measuring rod, the auxiliary measuring rod, the transverse measuring rod, the extension rod, the cross arm and the magnifier form an observation assembly.
The connecting pipe is connected with a conduit 7 through an upper pipe, as shown in fig. 17, two valves 71 are arranged left and right on the conduit, a culture solution barrel 73 is arranged between the two valves, the bottom of the culture solution barrel is communicated with the conduit, the valves are also arranged at the outlet of the culture solution barrel, and culture solution in the culture solution barrel can enter the conduit. A boss 72 is arranged between the culture solution barrel and the left valve, the boss is arranged above the guide pipe, the inner cavity of the boss is communicated with the guide pipe, the top of the boss is open, and a disinfection assembly is arranged on the boss.
As shown in fig. 18 and 19, the sterilizing assembly comprises a sterilizing lamp box 8, a plugboard 81, a sterilizing lamp 82, a switch 83, a conductive block 84, a positive electrode lead 85, a bottom plate 86, a floating plate 87, a conductive spring 88 and a negative electrode lead 89, wherein the sterilizing lamp box 8 is of a rectangular hollow structure and is open at the upper and lower sides, the plugboard 81 is fixed at the bottom of the sterilizing lamp box, and the plugboard is inserted on a boss during installation to realize the installation of the boss and the sterilizing lamp box. A bottom plate 86 is fixed in the inner cavity of the sterilizing lamp cartridge, and as shown in fig. 20, a plurality of floating plates 87 are arranged above the bottom plate, sterilizing lamps 82 are fixed on the floating plates, and conductive springs 88 are arranged between the floating plates and the bottom plate. The negative electrode of each disinfection lamp is electrically connected with a negative electrode lead 89, and a plurality of negative electrode leads are converged together and then are electrically connected with the negative electrode of the storage battery. The upper part of the inner cavity of the sterilizing lamp box is provided with a switch 83, which is a cuboid plastic piece as shown in fig. 19 to 21, and the front side and the rear side of the switch are respectively provided with a sliding block 831 which is in sliding connection with the sterilizing lamp box. A conductive block 84 is fixed at the bottom of the switch, and arc surfaces 841 are respectively arranged at the left and right sides of the bottom of the conductive block. In a natural state, the height of the lower surface of the conductive block is smaller than the height of the positive electrode of the sterilizing lamp. Pushing the switch to the left until the positive pole 821 of the sterilizing lamp contacts the conductive block, and compressing the conductive spring to make the sterilizing lamp move downwards; meanwhile, under the action of the conductive spring, the positive electrode of the sterilizing lamp is well contacted with the conductive block. The conductive block is connected with the positive electrode of the storage battery through the positive electrode wire 85, so that the storage battery, the positive electrode wire, the conductive block, the sterilizing lamp and the negative electrode wire form a sterilizing current loop. The section of the positive electrode lead, which is contacted with the conductive block, is spiral so as to adapt to the requirement of the conductive block when moving leftwards. The power of each disinfection lamp is different, and connects in parallel between a plurality of disinfection lamps, when one disinfection lamp is connected in the disinfection current loop, other disinfection lamps and disinfection current loop disconnection. When the switch is arranged at the rightmost side of the sterilizing lamp box, all sterilizing lamps are disconnected with the sterilizing current loop, namely the sterilizing assembly is in a non-working state; when the switch moves to the left side and contacts with the positive electrode of a certain disinfection lamp, the disinfection lamp is in a working state so as to disinfect tap water in the guide pipe below the boss. The arc surface on the lower surface of the conductive block is arranged, so that the positive electrode of the sterilizing lamp can be conveniently contacted with the conductive block.
The right end of the conduit is provided with a clasp 74 which is arranged to be connected with a water pipe, so that tap water in the water pipe enters the culture container through the conduit.
The following describes the method of use of the invention:
(1) The method comprises the steps of installing a supporting rod and a transition rod on a base, installing an observation assembly on an inner rod on the transition rod, installing a guide pipe on the base, and installing a disinfection assembly on a boss on the guide pipe;
(2) Placing the cloned plant in a culture container for culture, and placing stems of the cloned plant in a clamping plate;
(3) According to the requirement of the cloned plants on illumination intensity, rotating the lamp box to enable the corresponding light-transmitting optical filters to face downwards;
(4) According to the requirement of cultivating cloned plant, selecting whether to add tap water into the cultivation container; if tap water is needed to be added into the culture container, the clamping ring is connected with the water pipe, the two valves are opened, and tap water and culture solution are added into the culture container; if only the culture solution is needed to be added into the culture container, the right valve is closed, the left valve is opened, and the culture solution in the culture solution barrel enters the culture container; if only tap water is needed to be added into the culture container, closing a valve on the culture solution barrel and opening left and right valves, wherein tap water enters the culture container at the moment;
(5) In the process of cultivating the cloned plant, the length of the stem of the cloned plant is measured by a main measuring rod and an auxiliary measuring rod, and the outer diameter size of the stem of the plant or the root system width of the plant is measured by a transverse measuring rod and an extension rod; the appearance of the stem and root of the cloned plant was observed by a magnifying glass.

Claims (4)

1. The aquatic plant cloning character dynamic observation device is characterized by comprising a base, a support rod, a lamp post, an illumination assembly, a transition rod, an inner rod, an observation assembly, a guide pipe and a disinfection assembly, wherein the base is of a hollow structure with an open bottom, a clamping hole is formed in the top of the base, the clamping hole comprises a semicircular hole and a square hole which are arranged left and right and are communicated, a slot is formed in the inner wall of the clamping hole, a limiting plate is slidably arranged in the slot, a stem hole which is circular is formed between the limiting plate and the semicircular hole in a surrounding mode, and a clamping spring which is positioned in the slot is arranged between the limiting plate and the base;
the base is detachably provided with a support rod and a transition rod which are arranged up and down, the support rod is movably provided with a lamp post, the lamp post is provided with an illumination assembly with adjustable illumination intensity, and a locking bolt is arranged between the lamp post and the support rod;
an inner rod is movably arranged on the transition rod, a flexible damping sleeve is sleeved on the upper part of the inner rod, the upper part of the inner rod is arranged in the inner cavity of the transition rod, and the damping sleeve is tightly contacted with the inner wall of the transition rod; an observation assembly is detachably arranged at the lower part of the inner rod; the observation assembly comprises a main measuring rod, an auxiliary measuring rod movably arranged on the main measuring rod, a transverse measuring rod vertically arranged on the auxiliary measuring rod, an extension rod movably arranged on the transverse measuring rod, a cross arm arranged on the auxiliary measuring rod and a magnifying glass movably arranged on the cross arm; the outer wall of the upper part of the auxiliary measuring rod is sleeved with a flexible damping sleeve, a supporting lug is fixed on the transverse measuring rod, the supporting lug is arranged on the auxiliary measuring rod in a sliding manner, and a jackscrew is arranged between the supporting lug and the auxiliary measuring rod; a locking unit is arranged between the main measuring rod and the inner rod and comprises a locker fixed at the bottom of the inner rod, a baffle plate fixed in an inner cavity of the locker, a sliding plate arranged in the inner cavity of the locker in a sliding manner, a locking pin fixed on the sliding plate and a spring arranged between the sliding plate and the inner wall of the locker, the inner cavity of the locker is divided into a front part and a rear part by the baffle plate, the acting end of the locking pin stretches into the rear part of the inner cavity of the locker under the action of the spring, and a locking hole matched with the locking pin is formed in the top of the main measuring rod;
the base is detachably provided with a guide pipe, a pair of left and right valves are arranged on the guide pipe, a culture solution barrel communicated with the guide pipe is arranged between the two valves, a boss is arranged on the guide pipe, the boss is of a hollow structure with an open top, and a disinfection assembly is arranged on the boss; the disinfection assembly comprises a disinfection lamp box, a bottom plate fixed on the disinfection lamp box, a plurality of floating plates positioned above the bottom plate, a disinfection lamp fixed on the floating plates, a conductive spring arranged between the floating plates and the bottom plate, a negative electrode lead arranged between the negative electrode of the disinfection lamp and a storage battery, a switch movably arranged at the top of the disinfection lamp box, a conductive block arranged at the bottom of the switch, a positive electrode lead arranged between the conductive block and the positive electrode of the storage battery, and the height of the positive electrode of the disinfection lamp is larger than the height of the lower surface of the conductive block under the action of the conductive spring in a natural state, and the storage battery is arranged on a base.
2. The dynamic observation device for aquatic plant cloning property according to claim 1, wherein a fixed block is fixed in the inner cavity of the base, the support rod is in threaded connection with the upper part of the fixed block, and the transition rod is in threaded connection with the lower part of the fixed block.
3. The dynamic observation device for cloning characters of aquatic plants according to claim 1, wherein a plugboard is arranged at the bottom of the disinfection lamp box and is matched with the boss in a plug-in manner.
4. A method of operating the dynamic observation device for the cloning behavior of aquatic plants according to any one of claims 1 to 3, characterized in that it comprises the steps of:
(1) The method comprises the steps of installing a supporting rod and a transition rod on a base, installing an observation assembly on an inner rod on the transition rod, installing a guide pipe on the base, and installing a disinfection assembly on a boss on the guide pipe;
(2) Placing the cloned plant in a culture container for culture, and placing stems of the cloned plant in a clamping plate;
(3) According to the requirement of the cloned plants on illumination intensity, rotating the lamp box to enable the corresponding light-transmitting optical filters to face downwards;
(4) According to the requirement of cultivating cloned plant, selecting whether to add tap water into the cultivation container; if tap water is needed to be added into the culture container, the clamping ring is connected with the water pipe, the two valves are opened, and tap water and culture solution are added into the culture container; if only the culture solution is needed to be added into the culture container, the right valve is closed, the left valve is opened, and the culture solution in the culture solution barrel enters the culture container; if only tap water is needed to be added into the culture container, closing a valve on the culture solution barrel and opening left and right valves, wherein tap water enters the culture container at the moment;
(5) In the process of cultivating the cloned plant, the length of the stem of the cloned plant is measured by a main measuring rod and an auxiliary measuring rod, and the outer diameter size of the stem of the plant or the root system width of the plant is measured by a transverse measuring rod and an extension rod; the appearance of the stem and root of the cloned plant was observed by a magnifying glass.
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CN106499751A (en) * 2016-10-28 2017-03-15 北京机械设备研究所 A kind of anti-misoperation band is from spacing integration revolution shaft lock device
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