CN109554424A - The collagen Gly-His-Lys and preparation method thereof adjusted suitable for spacefarer's health - Google Patents

The collagen Gly-His-Lys and preparation method thereof adjusted suitable for spacefarer's health Download PDF

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Publication number
CN109554424A
CN109554424A CN201710880940.8A CN201710880940A CN109554424A CN 109554424 A CN109554424 A CN 109554424A CN 201710880940 A CN201710880940 A CN 201710880940A CN 109554424 A CN109554424 A CN 109554424A
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collagen
lys
health
spacefarer
preparation
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毛宏伟
曲建伟
周亮忠
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Beijing Flying Space Applied Technology Research Institute
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Beijing Flying Space Applied Technology Research Institute
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17Amino acids, peptides or proteins
    • A23L33/18Peptides; Protein hydrolysates

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Toxicology (AREA)
  • Biochemistry (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention belongs to polypeptide powder processing technique fields, more particularly to a kind of collagen Gly-His-Lys and preparation method thereof adjusted suitable for spacefarer's health, including pre-processing, going unless Collagen material, homogenate, extraction collagen, enzymatic hydrolysis, separation, ultrafiltration and drying process, wherein extract collagen be by 1:18-32g/mL solid-liquid ratio be added deionized water, and 60 DEG C -70 DEG C at a temperature of extract 10h-18h;Enzyme digestion reaction be papain is added with the amount of 5000U/g in the environment of pH is 6.5, and 40 DEG C -50 DEG C at a temperature of digest 2h-5h;The filter membrane specification used in ultra-filtration process includes 5000Da, 3000Da and 1000Da.Collagen Gly-His-Lys prepared by the present invention are not only easily absorbed but also significantly adjust phenomena such as astronaut causes internal metabolic disturbance, nutriment to be lost because of space environment, can utilize in the form of oral latex emulsion, compressed tablets, solid Instant Drinks, functional food and food additives etc..

Description

The collagen Gly-His-Lys and preparation method thereof adjusted suitable for spacefarer's health
Technical field
The invention belongs to polypeptide powder processing technique fields, and in particular to a kind of collagen egg adjusted suitable for spacefarer's health White Gly-His-Lys and preparation method thereof.
Background technique
During executing space mission, spacefarer will be subjected to space flight particular surroundings weightlessness, noise, vibration, radiation, round the clock The influence of the environmental factors such as rhythm and pace of moving things change, small space.These factors bring many bad shadows to the physiological system of spacefarer It rings.For example, the weightless myeloid tissue structure that can lead to spacefarer changes, denaturation and weight saving, thymic physiology occur for spleen Mitigate, and then cause the denaturation and atrophy of lymphoid tissue and organ, directly influences the execution of immune function.Influence exempting from for cell The non-specific immune function of epidemic disease function and human body weakens body to the resistance of bacterium, and the removing toxic substances cellulose content in blood is reduced, Lysozyme activity declines in saliva, and lymphocyte number is reduced, and interferon changes.By implementing scientific and reasonable diet battalion It supports and ensures, energy and nutrient needed for supplementing body can mitigate adverse effect brought by space environment factor, maintain boat The health of its member's body and mind.
Collagen is a kind of important functional protein, with hyperplasia, differentiation, movement, immune, joint lubrication, Wound healing etc. is closely related, directly or indirectly affects growth, aging and health of human body etc..In addition, collagen Since antigenicity is weaker, biocompatibility is preferable, and it is multiple to be widely used in medicine, food, daily-use chemical industry, biosynthesis etc. Industrial circle, such as health care product, medical capsule, surgical operation material, edible gelatin, photographic gelatin, cosmetics.
The collagen extracted from ox-hide, although nutrition very abundant, its unique three strands of superhelix makes Property it is sufficiently stable, cannot thoroughly be destroyed by general processing technology, cause its to digest and assimilate difficult, can not be abundant by human body It utilizes.However collagen is further hydrolyzed to can significantly improve its digestion and absorption, nutrition and function spy after collagen polypeptide Property etc..Therefore, ox-hide collagen polypeptide market prospects are very wide.
Past invention is concentrated on collagen.Such as patent CN103820517 discloses a kind of regulation The preparation method of the yak skin micromolecular collagen of human body energy metabolism, but the unresolved collagen property of this method is steady Fixed, human consumption absorbs difficulty, is unable to fully the defect utilized.Invention CN 101921311 discloses a kind of using Papain The method that enzyme enzymolysis of cow leather collagen prepares antifreeze peptide, but the purifying of this method includes Sephadex G-50 molecule The multiple technologies means such as sieve, Sepharose SP C-25 ion-exchange chromatography and RP-HPLC reversed-phase high performance liquid chromatography, cost Height is not suitable for the popularization and application of large area.
Summary of the invention
It is simple, effective suitable for spacefarer's health that technical problem to be solved by the invention is to provide a kind of methods Collagen Gly-His-Lys of adjusting and preparation method thereof.
To solve the above problems, technical solution provided by the invention is as follows.
Preparation method suitable for the collagen Gly-His-Lys that spacefarer's health is adjusted, it is characterised in that the following steps are included:
(1) the pretreatment of ox-hide: taking fresh ox-hide, and be cut into small pieces shape, is cleaned with after petroleum ether degreasing;
(2) go unless Collagen material: by clean ox-hide particle, after impregnating 10h-16h with NaCl, through 3-12 deionization Water is cleaned, is drained;
(3) it is homogenized: the ox-hide particle drained is crushed using high-speed organization;
(4) extract collagen: being gone into through step (3) processed sample by the solid-liquid ratio addition of 1:18-32 (g/mL) Ionized water, 60 DEG C -70 DEG C at a temperature of extract 10h-18h, after the completion of extraction 3000r/min be centrifuged 10-15min, supernatant It both is cow leather collagen;
(5) enzyme digestion reaction: it is 6.5 that the protein liquid of step (4), which is adjusted to pH, with acidic solution, and with the addition of the amount of 5000U/g Papain, 40 DEG C -50 DEG C at a temperature of digest 2h-5h;
Separation, ultrafiltration: enzymolysis liquid is centrifuged 10-30min under the revolving speed of 1000-2000r/min, after the completion of centrifugation on Clear liquid ultrafiltration membrance filter;
(7) dry: both obtaining collagen Gly-His-Lys after the filtrate of step (6) is freeze-dried.
Further, the present invention limits the homogenization process of step (3) using compartment, both stops after every homogenate 1min-3min Only 1min, until without blocky ox-hide particle.This is done to prevent the heat generated in homogenization process from causing ox-hide collagen The denaturation of albumen, to affect its quality.
Further, the present invention limits the revolving speed of step (4) extraction process as 1r/min-30r/min.Doing so both ensure that Sufficiently extract the formation for preventing emulsion again.
Further, the present invention limits the revolving speed of step step (5) enzymolysis process as 1r/min-15r/min.
Further, it includes HCl, C that the present invention, which limits (5) acidic solution that step is used in enzymolysis process,6H8O7、H3PO4
Further, the present invention limits step (6) the specification of filter membrane includes 5000Da, 3000Da, 1000Da in ultra-filtration process.
Using the collagen Gly-His-Lys for being suitable for spacefarer's health and adjusting of above method preparation.
Further, the present invention limits the prepared collagen Gly-His-Lys for being suitable for adjusting astronaut nutrition, purposes packet It includes and is applied to prepare oral latex emulsion, compressed tablets, solid Instant Drinks, functional food and food additives.
Application atrophy can occur for the muscle of astronaut in the space environment, lead to muscle loss, and skeletal muscle protein is closed At reduction, phenomena such as synthesis of body protein matter is reduced, which prevent space mission go on smoothly and the health of astronaut. The collagen Gly-His-Lys prepared through the invention are easily digested and assimilated by human body, are converted to participate in human body energy generation after absorption Enzyme needed for during thanking participates in intermediate product anabolic process, and then maintains vivo protein, fat, sugar and dimension The nutrient metabolics balance such as raw element, guarantees the normal operation of human body items physiological function, facilitates the suitable of astronaut's space mission Benefit carries out and the subsequent recovery being metabolized in vivo.
The organic principle of skeleton is mainly collagen, and the collagen reticular structure in bone is by mines such as calcium, phosphorus The adhesion of object ingredient, makes calcium combine closely with osteocyte, forms the toughness and hardness of bone.Collagen Gly-His-Lys of the invention point Son amount is low, and activity is high, digests and assimilates characteristic with preferable, it is increased by promotion Human osteoblast's cell after human intake absorbs It grows, increases osteoblast synthesis secretion collagen, the mineralization ability of osteocyte can be improved, there is enhancing bone density, improve bone Matter is loose, promotes the effect of bone injury healing, strong bone, in addition can promote teen-age bone growth and development, in improvement The bone health state of the elderly has highly important effect.
Specific embodiment
Further description of the technical solution of the present invention below, but is not so limited, all to the technology of the present invention Scheme is modified or replaced equivalently, and without departing from the spirit and scope of the technical solution of the present invention, should all be covered in the present invention Protection scope in.
Embodiment 1
Fresh ox-hide 500g is taken, be cut into small pieces shape, is cleaned with after petroleum ether degreasing.By clean ox-hide particle, NaCl is used After impregnating 10h, cleans, drains through 5 deionized waters.The ox-hide particle drained is crushed using high-speed organization, homogenization process is adopted It is compartment, both stopped 1min after every homogenate 2min, until without blocky ox-hide particle.This is done to prevent from being homogenized The heat generated in journey results in the denaturation of cow leather collagen, to affect its quality.Toward the homogenate of preprocessed mistake In liquid by 1:18 (g/mL) solid-liquid ratio be added deionized water, 60 DEG C at a temperature of extract 10h, constantly to be stirred during extraction It mixes, and revolving speed is maintained at 2r/min, does so and not only ensure that the formation sufficiently extracted but also prevent emulsion.After the completion of extraction It is centrifuged 10min under the revolving speed of 3000r/min, obtained supernatant is both cow leather collagen.With citric acid by the collagen of extraction Protein liquid pH is adjusted to 6.5, and papain is added to it with the amount of 5000U/g, 45 DEG C at a temperature of digest 2h, digested The revolving speed of journey is maintained at 2r/min.After the completion of enzyme digestion reaction, it is centrifuged 10min with the revolving speed of 1000r/min, centrifugate is used The membrane filtration of 1000Da both obtains collagen Gly-His-Lys after filtrate is freeze-dried.
Embodiment 2
Fresh ox-hide 500g is taken, be cut into small pieces shape, is cleaned with after petroleum ether degreasing.By clean ox-hide particle, NaCl is used After impregnating 16h, cleans, drains through 10 deionized waters.The ox-hide particle drained is crushed using high-speed organization, homogenization process is adopted It is compartment, both stopped 1min after every homogenate 3min, until without blocky ox-hide particle.Into the homogenate of preprocessed mistake By 1:32 (g/mL) solid-liquid ratio be added deionized water, 70 DEG C at a temperature of extract 18h, to be stirred continuously during extraction, and Revolving speed is maintained at 15r/min.It is centrifuged 10min under the revolving speed of 3000r/min after the completion of extraction, obtained supernatant is both ox-hide Collagen.The collagen liquid pH of extraction is adjusted to 6.5 with citric acid, and Papain is added to it with the amount of 5000U/g Enzyme, 50 DEG C at a temperature of digest 5h, the revolving speed of enzymolysis process is maintained at 2r/min.After the completion of enzyme digestion reaction, by its with The revolving speed of 1000r/min is centrifuged 10min, and the membrane filtration of centrifugate 3000Da both obtains collagen egg after filtrate is freeze-dried White Gly-His-Lys.
Embodiment 3
Fresh ox-hide 500g is taken, be cut into small pieces shape, is cleaned with after petroleum ether degreasing.By clean ox-hide particle, NaCl is used After impregnating 12h, cleans, drains through 12 deionized waters.The ox-hide particle drained is crushed using high-speed organization, homogenization process is adopted It is compartment, both stopped 1min after every homogenate 3min, until without blocky ox-hide particle.Into the homogenate of preprocessed mistake By 1:20g/mL solid-liquid ratio be added deionized water, 70 DEG C at a temperature of extract 18h, to be stirred continuously during extraction, and turn Speed is maintained at 15r/min.It is centrifuged 10min under the revolving speed of 3000r/min after the completion of extraction, obtained supernatant is both animal glue Former albumen.The collagen liquid pH of extraction is adjusted to 6.5 with hydrochloric acid, and papain is added to it with the amount of 5000U/g, 5h is digested at a temperature of 50 DEG C, the revolving speed of enzymolysis process is maintained at 2r/min.After the completion of enzyme digestion reaction, by it with 1000r/min Revolving speed be centrifuged 10min, the membrane filtration of centrifugate 5000Da, after filtrate is freeze-dried both collagen Gly-His-Lys.
Embodiment 4
Fresh ox-hide 500g is taken, be cut into small pieces shape, is cleaned with after petroleum ether degreasing.By clean ox-hide particle, NaCl is used After impregnating 10h, cleans, drains through 3 deionized waters.The ox-hide particle drained is crushed using high-speed organization, homogenization process is adopted It is compartment, both stopped 1min after every homogenate 3min, until without blocky ox-hide particle.Into the homogenate of preprocessed mistake By 1:25g/mL solid-liquid ratio be added deionized water, 65 DEG C at a temperature of extract 10h, to be stirred continuously during extraction, and turn Speed is maintained at 15r/min.It is centrifuged 10min under the revolving speed of 3000r/min after the completion of extraction, obtained supernatant is both animal glue Former albumen.The collagen liquid pH of extraction is adjusted to 6.5 with phosphoric acid, and papain is added to it with the amount of 5000U/g, 5h is digested at a temperature of 50 DEG C, the revolving speed of enzymolysis process is maintained at 2r/min.After the completion of enzyme digestion reaction, by it with 1000r/min Revolving speed be centrifuged 10min, the membrane filtration of centrifugate 5000Da, after filtrate is freeze-dried both collagen Gly-His-Lys.
Embodiment 5
The collagen Gly-His-Lys prepared using 1 the method for embodiment.
Embodiment 6
The collagen Gly-His-Lys prepared using 2 the method for embodiment.
Embodiment 7
The collagen Gly-His-Lys prepared using 3 the method for embodiment.
Embodiment 8
The collagen Gly-His-Lys prepared using 4 the method for embodiment.

Claims (8)

1. being suitable for the preparation method for the collagen Gly-His-Lys that spacefarer's health is adjusted, it is characterised in that the following steps are included:
(1) the pretreatment of ox-hide: taking fresh ox-hide, and be cut into small pieces shape, is cleaned with after petroleum ether degreasing;
(2) go unless Collagen material: clean ox-hide particle after impregnating 10h-16h with NaCl, is washed through 3-12 deionization Only, drain;
(3) it is homogenized: the ox-hide particle drained is crushed using high-speed organization;
(4) extract collagen: deionized water be added by the solid-liquid ratio of 1:18-32g/mL into through step (3) processed sample, 60 DEG C -70 DEG C at a temperature of extract 10h-18h, after the completion of extraction 3000r/min be centrifuged 10-15min, supernatant is both ox-hide Collagen;
(5) enzyme digestion reaction: it is 6.5 that the protein liquid of step (4), which is adjusted to pH, with acidic solution, and pawpaw is added with the amount of 5000U/g Protease, 40 DEG C -50 DEG C at a temperature of digest 2h-5h;
(6) enzymolysis liquid separation, ultrafiltration: is centrifuged 10-30min, supernatant after the completion of centrifugation under the revolving speed of 1000-2000r/min Use ultrafiltration membrance filter;
(7) dry: both obtaining collagen Gly-His-Lys after the filtrate of step (6) is freeze-dried.
2. the preparation method of the collagen Gly-His-Lys according to claim 1 adjusted suitable for spacefarer's health, feature It is, the homogenization process of step (3) both stopped 1min after every homogenate 1min-3min using compartment, until without blocky ox Skin particle.
3. the preparation method of the collagen Gly-His-Lys according to claim 1 adjusted suitable for spacefarer's health, feature Be, step (4) extraction process revolving speed be 1r/min-30r/min.
4. the preparation method of the collagen Gly-His-Lys according to claim 1 adjusted suitable for spacefarer's health, feature Be, step (5) enzymolysis process revolving speed be 1r/min-15r/min.
5. the preparation method of the collagen Gly-His-Lys according to claim 1 adjusted suitable for spacefarer's health, feature It is, (5) acidic solution that step is used in enzymolysis process includes HCl, C6H8O7、H3PO4
6. the preparation method of the collagen Gly-His-Lys according to claim 1 adjusted suitable for spacefarer's health, feature It is, (6) the specification of filter membrane includes 5000Da, 3000Da, 1000Da to step in ultra-filtration process.
7. using the collagen Gly-His-Lys for being suitable for spacefarer's health and adjusting of any the method preparation of claim 1 to 6.
8. the collagen Gly-His-Lys adjusted as claimed in claim 7 suitable for spacefarer's health, it is characterised in that be applied to system Standby oral latex emulsion, compressed tablets, solid Instant Drinks, functional food and food additives.
CN201710880940.8A 2017-09-26 2017-09-26 The collagen Gly-His-Lys and preparation method thereof adjusted suitable for spacefarer's health Pending CN109554424A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111011574A (en) * 2019-12-20 2020-04-17 杭州衡美食品科技有限公司 Preparation method and application of collagen peptide
CN112219958A (en) * 2020-07-08 2021-01-15 许昌神飞航天生物科技有限公司 Space navigation grade solid beverage for bone joint recuperation

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CN104087641A (en) * 2014-07-08 2014-10-08 天津大学 Yak skin blood pressure-decreasing collagen polypeptide and preparation method thereof

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Publication number Priority date Publication date Assignee Title
US20070219128A1 (en) * 2003-08-28 2007-09-20 Nanjing Besson Pharmacy Co., Ltd Medical and health-care uses of pufferfish type I collagen extract and processes for producing said extract
CN104087641A (en) * 2014-07-08 2014-10-08 天津大学 Yak skin blood pressure-decreasing collagen polypeptide and preparation method thereof

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111011574A (en) * 2019-12-20 2020-04-17 杭州衡美食品科技有限公司 Preparation method and application of collagen peptide
CN112219958A (en) * 2020-07-08 2021-01-15 许昌神飞航天生物科技有限公司 Space navigation grade solid beverage for bone joint recuperation

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Application publication date: 20190402