CN109554424A - The collagen Gly-His-Lys and preparation method thereof adjusted suitable for spacefarer's health - Google Patents
The collagen Gly-His-Lys and preparation method thereof adjusted suitable for spacefarer's health Download PDFInfo
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- 102000008186 Collagen Human genes 0.000 title claims abstract description 59
- 108010035532 Collagen Proteins 0.000 title claims abstract description 59
- 229920001436 collagen Polymers 0.000 title claims abstract description 59
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 title claims abstract description 29
- 108010038983 glycyl-histidyl-lysine Proteins 0.000 title claims abstract description 29
- 230000036541 health Effects 0.000 title claims abstract description 19
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 28
- 238000000605 extraction Methods 0.000 claims abstract description 17
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000006243 chemical reaction Methods 0.000 claims abstract description 7
- 238000001976 enzyme digestion Methods 0.000 claims abstract description 7
- 239000008367 deionised water Substances 0.000 claims abstract description 6
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 6
- 239000000839 emulsion Substances 0.000 claims abstract description 5
- 239000000463 material Substances 0.000 claims abstract description 4
- 239000007891 compressed tablet Substances 0.000 claims abstract description 3
- 235000013373 food additive Nutrition 0.000 claims abstract description 3
- 239000002778 food additive Substances 0.000 claims abstract description 3
- 235000013376 functional food Nutrition 0.000 claims abstract description 3
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- 239000002245 particle Substances 0.000 claims description 18
- 230000008569 process Effects 0.000 claims description 15
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 238000000265 homogenisation Methods 0.000 claims description 7
- 239000006228 supernatant Substances 0.000 claims description 7
- 238000005238 degreasing Methods 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 230000008520 organization Effects 0.000 claims description 6
- 239000003208 petroleum Substances 0.000 claims description 6
- 102000004169 proteins and genes Human genes 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 4
- 239000003929 acidic solution Substances 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000002242 deionisation method Methods 0.000 claims description 2
- 244000189799 Asimina triloba Species 0.000 claims 1
- 235000006264 Asimina triloba Nutrition 0.000 claims 1
- 235000009467 Carica papaya Nutrition 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 108090000526 Papain Proteins 0.000 abstract description 7
- 229940055729 papain Drugs 0.000 abstract description 7
- 235000019834 papain Nutrition 0.000 abstract description 7
- 229920001184 polypeptide Polymers 0.000 abstract description 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 4
- 230000002503 metabolic effect Effects 0.000 abstract description 2
- 238000009700 powder processing Methods 0.000 abstract description 2
- 238000001035 drying Methods 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract 1
- 238000007781 pre-processing Methods 0.000 abstract 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 210000000988 bone and bone Anatomy 0.000 description 4
- 238000004925 denaturation Methods 0.000 description 4
- 230000036425 denaturation Effects 0.000 description 4
- 239000010985 leather Substances 0.000 description 4
- 238000005374 membrane filtration Methods 0.000 description 4
- 239000003643 water by type Substances 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 235000016709 nutrition Nutrition 0.000 description 3
- 230000035764 nutrition Effects 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 206010003694 Atrophy Diseases 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 230000009102 absorption Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000037444 atrophy Effects 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 230000036737 immune function Effects 0.000 description 2
- 210000003205 muscle Anatomy 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 210000000963 osteoblast Anatomy 0.000 description 2
- 210000004409 osteocyte Anatomy 0.000 description 2
- 238000004007 reversed phase HPLC Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 108010053481 Antifreeze Proteins Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 102000008934 Muscle Proteins Human genes 0.000 description 1
- 108010074084 Muscle Proteins Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 206010061363 Skeletal injury Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000001195 anabolic effect Effects 0.000 description 1
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- 230000033228 biological regulation Effects 0.000 description 1
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- 230000007547 defect Effects 0.000 description 1
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- 235000005911 diet Nutrition 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
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- 230000035876 healing Effects 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 239000013067 intermediate product Substances 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 230000033001 locomotion Effects 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000005210 lymphoid organ Anatomy 0.000 description 1
- 210000003563 lymphoid tissue Anatomy 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
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- 210000002027 skeletal muscle Anatomy 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000002992 thymic effect Effects 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Toxicology (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Zoology (AREA)
- Gastroenterology & Hepatology (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention belongs to polypeptide powder processing technique fields, more particularly to a kind of collagen Gly-His-Lys and preparation method thereof adjusted suitable for spacefarer's health, including pre-processing, going unless Collagen material, homogenate, extraction collagen, enzymatic hydrolysis, separation, ultrafiltration and drying process, wherein extract collagen be by 1:18-32g/mL solid-liquid ratio be added deionized water, and 60 DEG C -70 DEG C at a temperature of extract 10h-18h;Enzyme digestion reaction be papain is added with the amount of 5000U/g in the environment of pH is 6.5, and 40 DEG C -50 DEG C at a temperature of digest 2h-5h;The filter membrane specification used in ultra-filtration process includes 5000Da, 3000Da and 1000Da.Collagen Gly-His-Lys prepared by the present invention are not only easily absorbed but also significantly adjust phenomena such as astronaut causes internal metabolic disturbance, nutriment to be lost because of space environment, can utilize in the form of oral latex emulsion, compressed tablets, solid Instant Drinks, functional food and food additives etc..
Description
Technical field
The invention belongs to polypeptide powder processing technique fields, and in particular to a kind of collagen egg adjusted suitable for spacefarer's health
White Gly-His-Lys and preparation method thereof.
Background technique
During executing space mission, spacefarer will be subjected to space flight particular surroundings weightlessness, noise, vibration, radiation, round the clock
The influence of the environmental factors such as rhythm and pace of moving things change, small space.These factors bring many bad shadows to the physiological system of spacefarer
It rings.For example, the weightless myeloid tissue structure that can lead to spacefarer changes, denaturation and weight saving, thymic physiology occur for spleen
Mitigate, and then cause the denaturation and atrophy of lymphoid tissue and organ, directly influences the execution of immune function.Influence exempting from for cell
The non-specific immune function of epidemic disease function and human body weakens body to the resistance of bacterium, and the removing toxic substances cellulose content in blood is reduced,
Lysozyme activity declines in saliva, and lymphocyte number is reduced, and interferon changes.By implementing scientific and reasonable diet battalion
It supports and ensures, energy and nutrient needed for supplementing body can mitigate adverse effect brought by space environment factor, maintain boat
The health of its member's body and mind.
Collagen is a kind of important functional protein, with hyperplasia, differentiation, movement, immune, joint lubrication,
Wound healing etc. is closely related, directly or indirectly affects growth, aging and health of human body etc..In addition, collagen
Since antigenicity is weaker, biocompatibility is preferable, and it is multiple to be widely used in medicine, food, daily-use chemical industry, biosynthesis etc.
Industrial circle, such as health care product, medical capsule, surgical operation material, edible gelatin, photographic gelatin, cosmetics.
The collagen extracted from ox-hide, although nutrition very abundant, its unique three strands of superhelix makes
Property it is sufficiently stable, cannot thoroughly be destroyed by general processing technology, cause its to digest and assimilate difficult, can not be abundant by human body
It utilizes.However collagen is further hydrolyzed to can significantly improve its digestion and absorption, nutrition and function spy after collagen polypeptide
Property etc..Therefore, ox-hide collagen polypeptide market prospects are very wide.
Past invention is concentrated on collagen.Such as patent CN103820517 discloses a kind of regulation
The preparation method of the yak skin micromolecular collagen of human body energy metabolism, but the unresolved collagen property of this method is steady
Fixed, human consumption absorbs difficulty, is unable to fully the defect utilized.Invention CN 101921311 discloses a kind of using Papain
The method that enzyme enzymolysis of cow leather collagen prepares antifreeze peptide, but the purifying of this method includes Sephadex G-50 molecule
The multiple technologies means such as sieve, Sepharose SP C-25 ion-exchange chromatography and RP-HPLC reversed-phase high performance liquid chromatography, cost
Height is not suitable for the popularization and application of large area.
Summary of the invention
It is simple, effective suitable for spacefarer's health that technical problem to be solved by the invention is to provide a kind of methods
Collagen Gly-His-Lys of adjusting and preparation method thereof.
To solve the above problems, technical solution provided by the invention is as follows.
Preparation method suitable for the collagen Gly-His-Lys that spacefarer's health is adjusted, it is characterised in that the following steps are included:
(1) the pretreatment of ox-hide: taking fresh ox-hide, and be cut into small pieces shape, is cleaned with after petroleum ether degreasing;
(2) go unless Collagen material: by clean ox-hide particle, after impregnating 10h-16h with NaCl, through 3-12 deionization
Water is cleaned, is drained;
(3) it is homogenized: the ox-hide particle drained is crushed using high-speed organization;
(4) extract collagen: being gone into through step (3) processed sample by the solid-liquid ratio addition of 1:18-32 (g/mL)
Ionized water, 60 DEG C -70 DEG C at a temperature of extract 10h-18h, after the completion of extraction 3000r/min be centrifuged 10-15min, supernatant
It both is cow leather collagen;
(5) enzyme digestion reaction: it is 6.5 that the protein liquid of step (4), which is adjusted to pH, with acidic solution, and with the addition of the amount of 5000U/g
Papain, 40 DEG C -50 DEG C at a temperature of digest 2h-5h;
Separation, ultrafiltration: enzymolysis liquid is centrifuged 10-30min under the revolving speed of 1000-2000r/min, after the completion of centrifugation on
Clear liquid ultrafiltration membrance filter;
(7) dry: both obtaining collagen Gly-His-Lys after the filtrate of step (6) is freeze-dried.
Further, the present invention limits the homogenization process of step (3) using compartment, both stops after every homogenate 1min-3min
Only 1min, until without blocky ox-hide particle.This is done to prevent the heat generated in homogenization process from causing ox-hide collagen
The denaturation of albumen, to affect its quality.
Further, the present invention limits the revolving speed of step (4) extraction process as 1r/min-30r/min.Doing so both ensure that
Sufficiently extract the formation for preventing emulsion again.
Further, the present invention limits the revolving speed of step step (5) enzymolysis process as 1r/min-15r/min.
Further, it includes HCl, C that the present invention, which limits (5) acidic solution that step is used in enzymolysis process,6H8O7、H3PO4。
Further, the present invention limits step (6) the specification of filter membrane includes 5000Da, 3000Da, 1000Da in ultra-filtration process.
Using the collagen Gly-His-Lys for being suitable for spacefarer's health and adjusting of above method preparation.
Further, the present invention limits the prepared collagen Gly-His-Lys for being suitable for adjusting astronaut nutrition, purposes packet
It includes and is applied to prepare oral latex emulsion, compressed tablets, solid Instant Drinks, functional food and food additives.
Application atrophy can occur for the muscle of astronaut in the space environment, lead to muscle loss, and skeletal muscle protein is closed
At reduction, phenomena such as synthesis of body protein matter is reduced, which prevent space mission go on smoothly and the health of astronaut.
The collagen Gly-His-Lys prepared through the invention are easily digested and assimilated by human body, are converted to participate in human body energy generation after absorption
Enzyme needed for during thanking participates in intermediate product anabolic process, and then maintains vivo protein, fat, sugar and dimension
The nutrient metabolics balance such as raw element, guarantees the normal operation of human body items physiological function, facilitates the suitable of astronaut's space mission
Benefit carries out and the subsequent recovery being metabolized in vivo.
The organic principle of skeleton is mainly collagen, and the collagen reticular structure in bone is by mines such as calcium, phosphorus
The adhesion of object ingredient, makes calcium combine closely with osteocyte, forms the toughness and hardness of bone.Collagen Gly-His-Lys of the invention point
Son amount is low, and activity is high, digests and assimilates characteristic with preferable, it is increased by promotion Human osteoblast's cell after human intake absorbs
It grows, increases osteoblast synthesis secretion collagen, the mineralization ability of osteocyte can be improved, there is enhancing bone density, improve bone
Matter is loose, promotes the effect of bone injury healing, strong bone, in addition can promote teen-age bone growth and development, in improvement
The bone health state of the elderly has highly important effect.
Specific embodiment
Further description of the technical solution of the present invention below, but is not so limited, all to the technology of the present invention
Scheme is modified or replaced equivalently, and without departing from the spirit and scope of the technical solution of the present invention, should all be covered in the present invention
Protection scope in.
Embodiment 1
Fresh ox-hide 500g is taken, be cut into small pieces shape, is cleaned with after petroleum ether degreasing.By clean ox-hide particle, NaCl is used
After impregnating 10h, cleans, drains through 5 deionized waters.The ox-hide particle drained is crushed using high-speed organization, homogenization process is adopted
It is compartment, both stopped 1min after every homogenate 2min, until without blocky ox-hide particle.This is done to prevent from being homogenized
The heat generated in journey results in the denaturation of cow leather collagen, to affect its quality.Toward the homogenate of preprocessed mistake
In liquid by 1:18 (g/mL) solid-liquid ratio be added deionized water, 60 DEG C at a temperature of extract 10h, constantly to be stirred during extraction
It mixes, and revolving speed is maintained at 2r/min, does so and not only ensure that the formation sufficiently extracted but also prevent emulsion.After the completion of extraction
It is centrifuged 10min under the revolving speed of 3000r/min, obtained supernatant is both cow leather collagen.With citric acid by the collagen of extraction
Protein liquid pH is adjusted to 6.5, and papain is added to it with the amount of 5000U/g, 45 DEG C at a temperature of digest 2h, digested
The revolving speed of journey is maintained at 2r/min.After the completion of enzyme digestion reaction, it is centrifuged 10min with the revolving speed of 1000r/min, centrifugate is used
The membrane filtration of 1000Da both obtains collagen Gly-His-Lys after filtrate is freeze-dried.
Embodiment 2
Fresh ox-hide 500g is taken, be cut into small pieces shape, is cleaned with after petroleum ether degreasing.By clean ox-hide particle, NaCl is used
After impregnating 16h, cleans, drains through 10 deionized waters.The ox-hide particle drained is crushed using high-speed organization, homogenization process is adopted
It is compartment, both stopped 1min after every homogenate 3min, until without blocky ox-hide particle.Into the homogenate of preprocessed mistake
By 1:32 (g/mL) solid-liquid ratio be added deionized water, 70 DEG C at a temperature of extract 18h, to be stirred continuously during extraction, and
Revolving speed is maintained at 15r/min.It is centrifuged 10min under the revolving speed of 3000r/min after the completion of extraction, obtained supernatant is both ox-hide
Collagen.The collagen liquid pH of extraction is adjusted to 6.5 with citric acid, and Papain is added to it with the amount of 5000U/g
Enzyme, 50 DEG C at a temperature of digest 5h, the revolving speed of enzymolysis process is maintained at 2r/min.After the completion of enzyme digestion reaction, by its with
The revolving speed of 1000r/min is centrifuged 10min, and the membrane filtration of centrifugate 3000Da both obtains collagen egg after filtrate is freeze-dried
White Gly-His-Lys.
Embodiment 3
Fresh ox-hide 500g is taken, be cut into small pieces shape, is cleaned with after petroleum ether degreasing.By clean ox-hide particle, NaCl is used
After impregnating 12h, cleans, drains through 12 deionized waters.The ox-hide particle drained is crushed using high-speed organization, homogenization process is adopted
It is compartment, both stopped 1min after every homogenate 3min, until without blocky ox-hide particle.Into the homogenate of preprocessed mistake
By 1:20g/mL solid-liquid ratio be added deionized water, 70 DEG C at a temperature of extract 18h, to be stirred continuously during extraction, and turn
Speed is maintained at 15r/min.It is centrifuged 10min under the revolving speed of 3000r/min after the completion of extraction, obtained supernatant is both animal glue
Former albumen.The collagen liquid pH of extraction is adjusted to 6.5 with hydrochloric acid, and papain is added to it with the amount of 5000U/g,
5h is digested at a temperature of 50 DEG C, the revolving speed of enzymolysis process is maintained at 2r/min.After the completion of enzyme digestion reaction, by it with 1000r/min
Revolving speed be centrifuged 10min, the membrane filtration of centrifugate 5000Da, after filtrate is freeze-dried both collagen Gly-His-Lys.
Embodiment 4
Fresh ox-hide 500g is taken, be cut into small pieces shape, is cleaned with after petroleum ether degreasing.By clean ox-hide particle, NaCl is used
After impregnating 10h, cleans, drains through 3 deionized waters.The ox-hide particle drained is crushed using high-speed organization, homogenization process is adopted
It is compartment, both stopped 1min after every homogenate 3min, until without blocky ox-hide particle.Into the homogenate of preprocessed mistake
By 1:25g/mL solid-liquid ratio be added deionized water, 65 DEG C at a temperature of extract 10h, to be stirred continuously during extraction, and turn
Speed is maintained at 15r/min.It is centrifuged 10min under the revolving speed of 3000r/min after the completion of extraction, obtained supernatant is both animal glue
Former albumen.The collagen liquid pH of extraction is adjusted to 6.5 with phosphoric acid, and papain is added to it with the amount of 5000U/g,
5h is digested at a temperature of 50 DEG C, the revolving speed of enzymolysis process is maintained at 2r/min.After the completion of enzyme digestion reaction, by it with 1000r/min
Revolving speed be centrifuged 10min, the membrane filtration of centrifugate 5000Da, after filtrate is freeze-dried both collagen Gly-His-Lys.
Embodiment 5
The collagen Gly-His-Lys prepared using 1 the method for embodiment.
Embodiment 6
The collagen Gly-His-Lys prepared using 2 the method for embodiment.
Embodiment 7
The collagen Gly-His-Lys prepared using 3 the method for embodiment.
Embodiment 8
The collagen Gly-His-Lys prepared using 4 the method for embodiment.
Claims (8)
1. being suitable for the preparation method for the collagen Gly-His-Lys that spacefarer's health is adjusted, it is characterised in that the following steps are included:
(1) the pretreatment of ox-hide: taking fresh ox-hide, and be cut into small pieces shape, is cleaned with after petroleum ether degreasing;
(2) go unless Collagen material: clean ox-hide particle after impregnating 10h-16h with NaCl, is washed through 3-12 deionization
Only, drain;
(3) it is homogenized: the ox-hide particle drained is crushed using high-speed organization;
(4) extract collagen: deionized water be added by the solid-liquid ratio of 1:18-32g/mL into through step (3) processed sample,
60 DEG C -70 DEG C at a temperature of extract 10h-18h, after the completion of extraction 3000r/min be centrifuged 10-15min, supernatant is both ox-hide
Collagen;
(5) enzyme digestion reaction: it is 6.5 that the protein liquid of step (4), which is adjusted to pH, with acidic solution, and pawpaw is added with the amount of 5000U/g
Protease, 40 DEG C -50 DEG C at a temperature of digest 2h-5h;
(6) enzymolysis liquid separation, ultrafiltration: is centrifuged 10-30min, supernatant after the completion of centrifugation under the revolving speed of 1000-2000r/min
Use ultrafiltration membrance filter;
(7) dry: both obtaining collagen Gly-His-Lys after the filtrate of step (6) is freeze-dried.
2. the preparation method of the collagen Gly-His-Lys according to claim 1 adjusted suitable for spacefarer's health, feature
It is, the homogenization process of step (3) both stopped 1min after every homogenate 1min-3min using compartment, until without blocky ox
Skin particle.
3. the preparation method of the collagen Gly-His-Lys according to claim 1 adjusted suitable for spacefarer's health, feature
Be, step (4) extraction process revolving speed be 1r/min-30r/min.
4. the preparation method of the collagen Gly-His-Lys according to claim 1 adjusted suitable for spacefarer's health, feature
Be, step (5) enzymolysis process revolving speed be 1r/min-15r/min.
5. the preparation method of the collagen Gly-His-Lys according to claim 1 adjusted suitable for spacefarer's health, feature
It is, (5) acidic solution that step is used in enzymolysis process includes HCl, C6H8O7、H3PO4。
6. the preparation method of the collagen Gly-His-Lys according to claim 1 adjusted suitable for spacefarer's health, feature
It is, (6) the specification of filter membrane includes 5000Da, 3000Da, 1000Da to step in ultra-filtration process.
7. using the collagen Gly-His-Lys for being suitable for spacefarer's health and adjusting of any the method preparation of claim 1 to 6.
8. the collagen Gly-His-Lys adjusted as claimed in claim 7 suitable for spacefarer's health, it is characterised in that be applied to system
Standby oral latex emulsion, compressed tablets, solid Instant Drinks, functional food and food additives.
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