CN109536575B - 一种淀粉酶的检测方法 - Google Patents
一种淀粉酶的检测方法 Download PDFInfo
- Publication number
- CN109536575B CN109536575B CN201811504097.4A CN201811504097A CN109536575B CN 109536575 B CN109536575 B CN 109536575B CN 201811504097 A CN201811504097 A CN 201811504097A CN 109536575 B CN109536575 B CN 109536575B
- Authority
- CN
- China
- Prior art keywords
- solution
- detected
- sample
- amylase
- starch
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 102000013142 Amylases Human genes 0.000 title claims abstract description 45
- 108010065511 Amylases Proteins 0.000 title claims abstract description 45
- 235000019418 amylase Nutrition 0.000 title claims abstract description 45
- 239000004382 Amylase Substances 0.000 title claims abstract description 44
- 238000001514 detection method Methods 0.000 title claims abstract description 33
- 229920002472 Starch Polymers 0.000 claims abstract description 61
- 239000008107 starch Substances 0.000 claims abstract description 61
- 235000019698 starch Nutrition 0.000 claims abstract description 60
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000008187 granular material Substances 0.000 claims abstract description 41
- UEJQQMLXZQUJHF-UHFFFAOYSA-L [K+].[I+].[I-].[I-] Chemical compound [K+].[I+].[I-].[I-] UEJQQMLXZQUJHF-UHFFFAOYSA-L 0.000 claims abstract description 26
- 238000004043 dyeing Methods 0.000 claims abstract description 3
- 239000011521 glass Substances 0.000 claims description 28
- 238000000034 method Methods 0.000 claims description 20
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 14
- 239000011248 coating agent Substances 0.000 claims description 14
- 238000000576 coating method Methods 0.000 claims description 14
- 239000006059 cover glass Substances 0.000 claims description 14
- 239000008103 glucose Substances 0.000 claims description 14
- 238000010438 heat treatment Methods 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims description 12
- 108010046377 Whey Proteins Proteins 0.000 claims description 11
- 102000007544 Whey Proteins Human genes 0.000 claims description 11
- 235000021552 granulated sugar Nutrition 0.000 claims description 11
- 238000002156 mixing Methods 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 11
- 235000021119 whey protein Nutrition 0.000 claims description 11
- 239000000203 mixture Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 4
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 claims description 3
- 235000001727 glucose Nutrition 0.000 claims description 3
- 229910052740 iodine Inorganic materials 0.000 claims description 3
- 239000011630 iodine Substances 0.000 claims description 3
- 239000002994 raw material Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000035484 reaction time Effects 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 235000013618 yogurt Nutrition 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000008034 disappearance Effects 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 229920001353 Dextrin Polymers 0.000 description 1
- 239000004375 Dextrin Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 229940025131 amylases Drugs 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 235000019425 dextrin Nutrition 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811504097.4A CN109536575B (zh) | 2018-12-10 | 2018-12-10 | 一种淀粉酶的检测方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811504097.4A CN109536575B (zh) | 2018-12-10 | 2018-12-10 | 一种淀粉酶的检测方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109536575A CN109536575A (zh) | 2019-03-29 |
CN109536575B true CN109536575B (zh) | 2022-07-05 |
Family
ID=65853320
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811504097.4A Active CN109536575B (zh) | 2018-12-10 | 2018-12-10 | 一种淀粉酶的检测方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109536575B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114324333A (zh) * | 2021-12-29 | 2022-04-12 | 光明乳业股份有限公司 | 一种常温酸奶原料中淀粉酶的检测方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6155960B2 (zh) * | 1977-10-07 | 1986-11-29 | Taito Fuaizaa Kk | |
JPS632598B2 (zh) * | 1984-08-24 | 1988-01-19 | Hitachi Plant Eng & Constr Co | |
CN104132939A (zh) * | 2014-07-12 | 2014-11-05 | 福建农林大学 | 一种蜂蜜中淀粉酶值的快速检测方法 |
-
2018
- 2018-12-10 CN CN201811504097.4A patent/CN109536575B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6155960B2 (zh) * | 1977-10-07 | 1986-11-29 | Taito Fuaizaa Kk | |
JPS632598B2 (zh) * | 1984-08-24 | 1988-01-19 | Hitachi Plant Eng & Constr Co | |
CN104132939A (zh) * | 2014-07-12 | 2014-11-05 | 福建农林大学 | 一种蜂蜜中淀粉酶值的快速检测方法 |
Non-Patent Citations (1)
Title |
---|
非晶颗粒态木薯淀粉的结构及酶降解活性研究;梁勇;《食品工业科技》;20040925;第25卷(第9期);第49-51页 * |
Also Published As
Publication number | Publication date |
---|---|
CN109536575A (zh) | 2019-03-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104833671B (zh) | 一种稻米绝对直链淀粉含量的测定方法 | |
Nielsen | Rapid determination of starch-An index to maturity in starchy vegetables | |
CN104502618B (zh) | 一种多比色皿自动分析系统及比色分析方法和应用 | |
CN106715670A (zh) | 使用红外光谱法在线监测糖化过程的方法 | |
CN109536575B (zh) | 一种淀粉酶的检测方法 | |
CN102042979A (zh) | 一种淀粉发酵液中葡萄糖含量的快速检测方法 | |
CN105755103A (zh) | 一种稳定的酶法测定脂肪酶活性的试剂 | |
CN110044894B (zh) | 一种三唑醇的比色检测方法 | |
CN101586146B (zh) | 一种检测土壤纤维素酶活性的分析方法 | |
CN106353309B (zh) | 一种检测调制乳中酵母β-葡聚糖含量的方法 | |
CN100507006C (zh) | 蔗糖工业用复合酶制剂 | |
CN104568804A (zh) | 流域总氮总磷检测方法 | |
CN111999276B (zh) | 一种制备发光铕基金属有机框架探针的方法及其应用 | |
CN102323261B (zh) | 一种海洋微藻细胞内淀粉含量的测定方法 | |
CN105842037A (zh) | 一种同时显示肥大细胞与嗜酸性细胞的染色方法 | |
CN109001189B (zh) | 检测甘蔗制的糖品中的淀粉酶含量范围的方法及其应用 | |
PL80211B1 (zh) | ||
CN212514113U (zh) | 一种分析酿酒高粱淀粉中水分、透明度、冻融性的系统 | |
CN107741491A (zh) | 一种测定尿半乳糖的试剂盒及其制备使用方法 | |
CN109406512B (zh) | 一种以黄原胶为原料的生产工艺中淀粉酶的检测方法 | |
CN106053364A (zh) | 一种测定胰岛素的试剂盒及其制备方法 | |
CN110133179A (zh) | 一种还原糖检测试纸及其使用方法 | |
EP1762843A1 (en) | Metal indicator | |
CN106645126B (zh) | 鉴别糯高粱和粳高粱的方法 | |
CN110333329A (zh) | 酿酒用粮食中支链淀粉含量的快速检测方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: 050000 No. 68 stone Copper Road, Hebei, Shijiazhuang Applicant after: JUNLEBAO Dairy Group Co.,Ltd. Address before: 050000 No. 68 stone Copper Road, Hebei, Shijiazhuang Applicant before: THE SHIJIAZHUANG JUNLEBAO DAIRY Co.,Ltd. |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP03 | Change of name, title or address |
Address after: 050000 No. 68 stone Copper Road, Hebei, Shijiazhuang Patentee after: Junlebao Dairy Group Co.,Ltd. Country or region after: China Address before: 050000 No. 68 stone Copper Road, Hebei, Shijiazhuang Patentee before: JUNLEBAO Dairy Group Co.,Ltd. Country or region before: China |