CN109526742A - 一种日本红枫青龙组培苗继代增殖培养基配方 - Google Patents

一种日本红枫青龙组培苗继代增殖培养基配方 Download PDF

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CN109526742A
CN109526742A CN201811593494.3A CN201811593494A CN109526742A CN 109526742 A CN109526742 A CN 109526742A CN 201811593494 A CN201811593494 A CN 201811593494A CN 109526742 A CN109526742 A CN 109526742A
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green dragon
tissue
japanese red
red maple
culture medium
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孙红英
辛全伟
罗海凌
严少娟
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Fujian Agriculture and Forestry University
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Fujian Agriculture and Forestry University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001—Culture apparatus for tissue culture
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

本发明属于植物培养基配方技术领域，具体涉及一种日本红枫青龙组培苗继代培养基增殖配方。所述培养基包含成分和含量为：WPM+0.06 mg L‑1 TDZ+0.3 mg L‑1 IBA+琼脂6.5 g L‑1+蔗糖30 g L‑1。本发明所制成的培养基能使日本红枫青龙组培过程的增殖倍数增大，增殖系数高达4.8；培养基配方制作方法简单，原料来源广泛，价格低廉，极大的节省了生产成本，可进行大面积的推广；本发明培养基可以显著提高日本红枫青龙增殖系数及扦插成活率，同时能够有效降低污染率，从而满足继代增殖的需要，促进快繁体系的建立。

Description

一种日本红枫青龙组培苗继代增殖培养基配方
技术领域
本发明属于植物培养基配方技术领域，具体涉及一种日本红枫青龙组培苗继代培养基配方。
背景技术
日本红枫青龙羽毛枫（Acer palmatum ‘seiryu’）是1965年由荷兰鹿特丹Trompenburg树木园的J. R. Pn Hoey Smith培育的新品种，在欧美大量运用。该品种于1984年得到英国皇家园艺协会“提名荣誉”奖，被欧美园艺界公论为近几十年来杰出的新品种。青龙羽毛枫为落叶小乔木，高可达8米，此品种虽然有着秀丽的叶形，属于羽毛枫类，但茎杆直立、不下垂。叶色春季嫩绿，夏季亮绿，秋季转为鲜艳的红铜色，夏季偶有焦叶现象，叶掌深裂几达基部，为7深裂-11深裂，裂片狭长又有羽状细裂，具细叶齿。生长势强，速度快，树姿优美，无论是孤植、群植还是作为行道树种植，能以“红袖善舞翠云间”的火热魅力，让人们陶醉其中，怡然自得，是优秀的园林观叶植物新品种。若以常绿树或白色墙体为背景，景观尤为美丽。但是由于青龙羽毛枫繁殖较为困难，扦插成活率低。目前，青龙繁殖以嫁接为主，但由于砧木与接穗的亲和关系，嫁接成活率不理想。
发明内容
本发明的目的在于提供一种日本红枫青龙组培苗继代增殖培养基配方。本发明通过植物组培快繁技术，建立起青龙组培快繁体系，成活率达到90％以上，为工厂化育苗提供技术支撑。
为实现上述目的，本发明采用如下技术方案：
所述日本红枫青龙组培苗继代增殖培养基配方，所述培养基包含成分和含量为：WPM +0.06 mg L-1 TDZ + 0.3 mg L-1 IBA + 琼脂6.5 g L-1 + 蔗糖30 g L-1，pH5.8。
本发明的显著优点在于：
（1）本发明所制成的培养基能使日本红枫青龙组培过程的增殖倍数增大，增殖系数高达4.8；
（2）本发明培养基配方制作方法简单，原料来源广泛，价格低廉，极大的节省了生产成本，可进行大面积的推广；
（3）本发明培养基可以显著提高日本红枫青龙增殖系数及扦插成活率，同时能够有效降低污染率，从而满足继代增殖的需要，促进快繁体系的建立。
具体实施方式
为进一步公开而不是限制本发明，以下结合实例对本发明作进一步的详细说明。
实施例1
所述日本红枫青龙组培苗继代增殖培养基配方，采用L9 (34) 正交试验设计，基本培养基为WPM木本培养基，激素TDZ为三个水平：0.04 mg L-1, 0.06 mg L-1, 0.08 mg L-1。生长素IBA三个浓度梯度为：0.1 mg L-1, 0.3 mg L-1, 0.5 mg L-1。蔗糖分别为：10 g L-1, 20g L-1, 30 g L-1。琼脂6.5 g L-1。
表1 不同培养基对青龙增值系数的正交试验设计
表2 不同植物生长物质对青龙增值的影响
最终经过筛选出青龙增值最佳培养基为：WPM + 0.06 mg L-1 TDZ + 0.3 mg L-1 IBA +琼脂6.5 g L-1 + 蔗糖30 g L-1，增殖系数高达4.8。
对照组：以MS为基本培养基，其配方为MS+0.06 mg L-1 TDZ + 0.3 mg L-1 IBA +琼脂6.5 g L-1 + 蔗糖30 g L-1，其增值系数仅为1.5，且分化苗较细弱，生长缓慢。
目前，对日本红枫组培有少许报道，但针对日本红枫青龙品种的组培研究至今未见报道。本研究从获得青龙无菌外植体、启动培养、继代培养、生根诱导及炼苗移栽，成功建立了青龙组培快繁体系，为青龙工厂化育苗奠定理论基础和技术支持。
以上所述仅为本发明的较佳实施例，凡依本发明申请专利范围所做的均等变化与修饰，皆应属本发明的涵盖范围。

Claims (2)

1.一种日本红枫青龙组培苗继代增殖培养基配方，其特征在于：所述培养基配方包括WPM木本培养基、激素TDZ、生长素IBA、琼脂、蔗糖。
2.根据权利要求1所述的一种日本红枫青龙组培苗继代增殖培养基配方，其特征在于：所述培养基配方包含成分和含量具体为：WPM + 0.06 mg L-1 TDZ + 0.3 mg L-1 IBA + 琼脂6.5 g L-1 + 蔗糖30 g L-1，pH5.8。
CN201811593494.3A 2018-12-25 2018-12-25 一种日本红枫青龙组培苗继代增殖培养基配方 Pending CN109526742A (zh)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101578963A (zh) * 2009-06-10 2009-11-18 重庆科技学院 日本红枫的组织培养快繁方法
CN104663450A (zh) * 2015-03-05 2015-06-03 罗焕荣 一种美国红枫组培快繁方法
CN106332777A (zh) * 2016-08-24 2017-01-18 盛世绿源科技有限公司 一种红花槭的组培方法
KR20180064190A (ko) * 2016-12-05 2018-06-14 한국콜마주식회사 섬단풍나무의 캘러스 유도 또는 증식용 배지 및 이를 이용한 캘러스 유도 또는 증식방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101578963A (zh) * 2009-06-10 2009-11-18 重庆科技学院 日本红枫的组织培养快繁方法
CN104663450A (zh) * 2015-03-05 2015-06-03 罗焕荣 一种美国红枫组培快繁方法
CN106332777A (zh) * 2016-08-24 2017-01-18 盛世绿源科技有限公司 一种红花槭的组培方法
KR20180064190A (ko) * 2016-12-05 2018-06-14 한국콜마주식회사 섬단풍나무의 캘러스 유도 또는 증식용 배지 및 이를 이용한 캘러스 유도 또는 증식방법

Non-Patent Citations (2)

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Title
MANUELA ELENA CONCIOIU等: "BIOTIC AND ABIOTIC FACTORS INFLUENCE DURING THE IN VITRO MULTIPLICATION PHASE OF SOME SPECIES AND VARIETIES OF THE ACER GENUS", 《CURRENT TRENDS IN NATURAL SCIENCES》 *
孙红英等: "日本红枫‘青龙’组织培养与快速繁殖", 《中南林业科技大学学报》 *

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