CN109496709B - Method for strengthening and promoting bud of pseudobulb bletillae tissue culture seedling in temporary planting - Google Patents
Method for strengthening and promoting bud of pseudobulb bletillae tissue culture seedling in temporary planting Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000001737 promoting effect Effects 0.000 title claims abstract description 14
- 238000005728 strengthening Methods 0.000 title abstract description 5
- 239000000411 inducer Substances 0.000 claims abstract description 51
- 238000005507 spraying Methods 0.000 claims abstract description 34
- 241001313857 Bletilla striata Species 0.000 claims abstract description 27
- 239000003337 fertilizer Substances 0.000 claims abstract description 21
- 239000000758 substrate Substances 0.000 claims abstract description 21
- 241001313855 Bletilla Species 0.000 claims abstract description 10
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 19
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 19
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 19
- GEWDNTWNSAZUDX-WQMVXFAESA-N (-)-methyl jasmonate Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-WQMVXFAESA-N 0.000 claims description 7
- RMOGWMIKYWRTKW-UONOGXRCSA-N (S,S)-paclobutrazol Chemical compound C([C@@H]([C@@H](O)C(C)(C)C)N1N=CN=C1)C1=CC=C(Cl)C=C1 RMOGWMIKYWRTKW-UONOGXRCSA-N 0.000 claims description 7
- 239000005979 Forchlorfenuron Substances 0.000 claims description 7
- 239000005985 Paclobutrazol Substances 0.000 claims description 7
- GPXLRLUVLMHHIK-UHFFFAOYSA-N forchlorfenuron Chemical compound C1=NC(Cl)=CC(NC(=O)NC=2C=CC=CC=2)=C1 GPXLRLUVLMHHIK-UHFFFAOYSA-N 0.000 claims description 7
- GEWDNTWNSAZUDX-UHFFFAOYSA-N methyl 7-epi-jasmonate Natural products CCC=CCC1C(CC(=O)OC)CCC1=O GEWDNTWNSAZUDX-UHFFFAOYSA-N 0.000 claims description 7
- 230000005059 dormancy Effects 0.000 claims description 5
- 230000035784 germination Effects 0.000 claims description 5
- 235000015097 nutrients Nutrition 0.000 claims description 5
- 239000000463 material Substances 0.000 abstract description 3
- 230000001965 increasing effect Effects 0.000 description 9
- 241000196324 Embryophyta Species 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 230000000694 effects Effects 0.000 description 4
- 235000000292 Gouania lupuloides Nutrition 0.000 description 3
- 241001492410 Pratia purpurascens Species 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- NNBFNNNWANBMTI-UHFFFAOYSA-M brilliant green Chemical compound OS([O-])(=O)=O.C1=CC(N(CC)CC)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](CC)CC)C=C1 NNBFNNNWANBMTI-UHFFFAOYSA-M 0.000 description 2
- 208000034507 Haematemesis Diseases 0.000 description 1
- 208000000616 Hemoptysis Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 206010040849 Skin fissures Diseases 0.000 description 1
- 206010053476 Traumatic haemorrhage Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 229930002877 anthocyanin Natural products 0.000 description 1
- 235000010208 anthocyanin Nutrition 0.000 description 1
- 239000004410 anthocyanin Substances 0.000 description 1
- 150000004636 anthocyanins Chemical class 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000014634 leaf senescence Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 150000001629 stilbenes Chemical class 0.000 description 1
- 235000021286 stilbenes Nutrition 0.000 description 1
- 230000035882 stress Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
- 239000009977 weikangning Substances 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
- 239000009306 yunnan baiyao Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention discloses a method for strengthening and promoting buds of pseudobulb bletilla striata tissue culture seedlings by temporary planting, which comprises the following steps: (1) temporarily planting the rhizoma bletillae tissue culture seedlings on a substrate for about 7 days, spraying a foliar fertilizer, and spraying the foliar fertilizer again in 14 days; (2) spraying the strong seedling inducer solution on the leaves after temporary planting until white new roots grow on the root tips, and spraying for 1 time after 7 days; (3) temporarily planting until the color of the seedling leaves is dark green, spraying the bud inducer solution on the leaves, and spraying for 1 time after 7 days; (4) after the seedlings are temporarily planted until the seedlings are poured successively, the seedlings enter a dormant state, and the pseudobulbs begin to sprout to emit bud tips; when the dormant period of the bletilla pseudobulb is ended and a third leaf grows out, repeating the steps (2) and (3), and spraying a strong seedling inducer and a bud inducer; after the temporary planting is finished for 6 months, the nursery can be released. The method disclosed by the invention is simple to operate, efficient and stable, the planting period is shortened, the output value of a unit area of land is improved, manpower and material resources are saved, and the planting cost is greatly reduced.
Description
Technical Field
The invention relates to the field of plant tissue culture, in particular to a method for strengthening and promoting buds of rhizoma bletillae tissue culture seedlings by temporary planting.
Background
Rhizoma Bletillae (Bletilla striata(Thunb.) Reichb.f.) is a perennial herb of Orchidaceae, has high ornamental value, is a traditional Chinese medicine in China, is a traditional beautifying and whitening medicine, is recorded in Shennong's herbal classic and is included in pharmacopoeia of the people's republic of China, and has a long medicinal history. Rhizoma Bletillae is slightly cold in nature, bitter and sweet in taste, has effects of astringing to stop bleeding, detumescence and promoting granulation, and can be used for treating hemoptysis, hematemesis, traumatic hemorrhage, pyocutaneous disease and toxic swelling, chapped skin, etc. The bletilla striata compounds mainly comprise nearly fifty kinds of stilbenes, steroids, triterpenes, anthocyanins and the like, and are the main components of Chinese patent medicines such as 'Kuaiwei tablets', 'Weikangning', 'Weile' and 'Yunnan Baiyao'. Modern researches have shown that rhizoma Bletillae extract has no adverse side effects, and has been added into medicinal and edible Chinese medicinal materials for health promotion, skin care and daily useThe use will be further broadened.
The rhizoma bletillae native production in China is distributed in regions of south China in Dabie mountain areas of Henan, the yield is low although the distribution range is wide, and the rhizoma bletillae medicinal raw materials are mainly wild for a long time and the wild resources are nearly exhausted. With the increasing market demand of rhizoma bletillae, the rhizoma bletillae is planted in a large scale at present. The time from rhizoma bletillae tissue culture seedling to product harvest is 5 years, the yield can reach high yield, the period is long, a large amount of land resources are occupied, and the planting cost is increased. How to shorten the planting time of rhizoma bletillae tissue culture seedlings, reduce the planting cost and improve the market competitiveness is a problem in the development process of the rhizoma bletillae industry.
Disclosure of Invention
The invention aims to solve the problem that the time required for achieving high yield of bletilla striata tissue culture seedlings is long at present, and provides a method for promoting strong seedlings to bud by pseudobulbus bletillae tissue culture seedlings in a temporary planting mode, so that the planting time of the bletilla striata tissue culture seedlings is shortened.
Since the bletilla striata yield is composed of the number of pseudobulbs and the weight of a single pseudobulb, and the weight of a single pseudobulb is determined by the bletilla striata seed source, under the condition of the same seed source, the bletilla striata yield is increased by increasing the number of the pseudobulbs. The number of bletilla pseudobulbs depends on the number of bletilla buds, since the base of each bud of bletilla pseudobulb forms a pseudobulb. The buds of the bletilla striata grow out on the pseudobulb of the previous year basically, so the pseudobulb of the first year is more, and the number of the bletilla striata buds of the next year is more for a long time. Based on the growth characteristic of bletilla striata, when the tissue culture seedlings of bletilla striata are transferred from a bottle to a seedbed for temporary planting, strong seedlings are carried out, the number of buds is increased, the number of pseudo-bulbs in the temporary planting period of the general tissue culture seedlings is 1-2, the seedlings in the temporary planting period are treated by using a chemical inducer, and the number of the pseudo-bulbs of each pseudo-planted seedling after each plant leaves the nursery is increased by increasing the number of the buds. When the bletilla pseudobulb seedlings are transplanted to a field for planting, the pseudobulb number of each seedling is large, so that the number of buds growing from the pseudobulb in the same year is increased, the high yield of the seedlings can be achieved after 3 years of planting, the planting time is greatly shortened, the planting cost is reduced, and the market competitiveness is improved.
The technical scheme for realizing the purpose of the invention is as follows:
a method for promoting strong seedlings and sprouting in pseudobulb tissue culture seedling temporary planting comprises the following steps:
(1) the bletilla striata tissue culture seedlings are temporarily planted on the substrate for about 7 days, the foliar fertilizer is sprayed, and the foliar fertilizer is sprayed once again in 14 days, so that the effects of enhancing the stress resistance of the bletilla striata tissue culture seedlings, promoting the root system to restore the activity, promoting the growth of pseudobulbs and delaying the leaf senescence of the bletilla striata tissue culture seedlings are mainly achieved;
the foliar fertilizer is 2 per mill potassium dihydrogen phosphate solution;
(2) when the tissue culture seedlings of the bletilla striata are heeled in on a substrate until white new roots grow on the root tips, the strong seedling inducer solution is sprayed on the leaves, and is sprayed for 1 time after 7 days, so that nutrients can be gathered to the pseudobulb parts, and the expansion of the pseudobulbs is promoted;
the strong seedling inducer consists of 2 per mill potassium dihydrogen phosphate solution and 50-200 mg/L paclobutrazol, and the pH value is 7.0;
(3) temporarily planting rhizoma bletillae tissue culture seedlings on a substrate until the interval between the time when the rhizoma bletillae tissue culture seedlings and the last 1 time of spraying of the strong seedling inducer in the step (2) is 10 days, enabling the leaves of the seedlings to be greenish, spraying a bud inducer solution on the leaves, and spraying 1 time after 7 days to promote the bud germination on pseudobulbs;
the bud inducer consists of 2 per mill potassium dihydrogen phosphate solution, 10-50 mg/L forchlorfenuron and 10-50 mu mol/L methyl jasmonate, and the pH value is 7.0;
(4) the rhizoma bletillae tissue culture seedlings are temporarily planted on the substrate until the seedlings are poured successively, the rhizoma bletillae tissue culture seedlings enter a dormant state, and pseudobulbs begin to sprout to emit bud tips; after the dormancy period of the bletilla pseudobulb is finished, the bud tips begin to grow, more than 2 buds grow on each seedling, leaves grow, when the third leaf grows, the steps (2) and (3) are repeated, and a strong seedling inducer and a bud inducer are sprayed; after the temporary planting is finished for 6 months, the temporary planting period of the rhizoma bletillae tissue culture seedlings is finished, and the rhizoma bletillae can be outplanted.
In the method, the seedling strengthening inducer in the step (2) preferably consists of 2 per mill potassium dihydrogen phosphate solution and 100mg/L paclobutrazol, and the pH value is 7.0;
in the above method, the bud inducer in step (3) preferably comprises 2 ‰ potassium dihydrogen phosphate solution, 30mg/L forchlorfenuron, and 20 μmol/L methyl jasmonate, and has pH of 7.0.
Watering for 1 time each morning, applying biogas rich water with lower concentration 20 days after temporary planting, and applying biogas rich water with the same concentration 1 time every 7 days; the spraying of the foliar fertilizer and the inducer is carried out after 4 pm.
The method disclosed by the invention is simple to operate, efficient and stable, the planting period is shortened, the output value of a unit area of land is improved, manpower and material resources are saved, and the planting cost is greatly reduced.
Detailed Description
Example 1:
a method for promoting strong seedlings and sprouting in pseudobulb tissue culture seedling temporary planting comprises the following steps:
(1) temporarily planting the rhizoma bletillae tissue culture seedlings on a substrate for about 7 days, spraying a foliar fertilizer, and spraying the foliar fertilizer again in 14 days; the foliar fertilizer is 2 per mill potassium dihydrogen phosphate solution;
(2) when the bletilla striata tissue culture seedlings are temporarily planted on the substrate for 21 days, new white roots grow at the root tips of the seedlings, a strong seedling inducer solution is sprayed on leaves, and the strong seedling inducer solution is sprayed for 1 time after 7 days, so that nutrients are favorably gathered to pseudobulb parts, and the expansion of the pseudobulbs is promoted; the strong seedling inducer consists of 2 per mill potassium dihydrogen phosphate solution and 50mg/L paclobutrazol, and the pH value is 7.0;
(3) temporarily planting rhizoma bletillae tissue culture seedlings on a substrate until the interval between the time when the rhizoma bletillae tissue culture seedlings and the last 1 time of spraying of the strong seedling inducer in the step (2) is 10 days, enabling the leaves of the seedlings to be cyan, spraying a bud inducer solution on the leaves, and spraying 1 time after 7 days to promote the bud germination on pseudobulbs; the bud inducer consists of 2 per mill potassium dihydrogen phosphate solution, 10mg/L forchlorfenuron and 10 mu mol/L methyl jasmonate, and the pH value is 7.0;
(4) after the rhizoma bletillae tissue culture seedlings are temporarily planted on the substrate for 70-90 days, the seedlings begin to fall down and enter a dormant state, and buds on the pseudobulbs sprout to shoot bud tips; after the dormancy period of the bletilla pseudobulb is finished, the bud tip begins to grow, a first leaf grows, when a third leaf grows, the steps (2) and (3) are repeated, and a strong seedling inducer and a bud inducer are sprayed; after the temporary planting is finished for 6 months, the temporary planting process of the rhizoma bletillae tissue culture seedlings is finished, and the rhizoma bletillae can be outplanted; the seedlings in the nursery are slightly short, generally strong, and have cyan leaves, the average pseudobulb number is 1.8 per plant, the average pseudobulb size is 1.28 cm, and the average bud number is 5.5 per plant.
Watering for 1 time each morning, applying biogas rich water with lower concentration 20 days after temporary planting, and applying biogas rich water with the same concentration 1 time every 7 days; the spraying of the foliar fertilizer and the inducer is carried out after 4 pm.
Example 2:
a method for promoting strong seedlings and sprouting in pseudobulb tissue culture seedling temporary planting comprises the following steps:
(1) temporarily planting the rhizoma bletillae tissue culture seedlings on a substrate for about 7 days, spraying a foliar fertilizer, and spraying the foliar fertilizer again in 14 days; the foliar fertilizer is 2 per mill potassium dihydrogen phosphate solution;
(2) when the bletilla striata tissue culture seedlings are temporarily planted on the substrate for 21 days, new white roots grow at the root tips of the seedlings, a strong seedling inducer solution is sprayed on leaves, and the strong seedling inducer solution is sprayed for 1 time after 7 days, so that nutrients are favorably gathered to pseudobulb parts, and the expansion of the pseudobulbs is promoted; the strong seedling inducer consists of 2 per mill potassium dihydrogen phosphate solution and 200mg/L paclobutrazol, and the pH value is 7.0;
(3) temporarily planting rhizoma bletillae tissue culture seedlings on a substrate until the interval between the temporary planting of rhizoma bletillae tissue culture seedlings and the last 1-time spraying of the strong seedling inducer in the step (2) is 10 days, enabling the leaves of the seedlings to be emerald green, spraying a bud inducer solution on the leaves, and spraying 1 time after 7 days to promote the bud germination on pseudobulbs; the bud inducer consists of 2 per mill potassium dihydrogen phosphate solution, 50mg/L forchlorfenuron and 50 mu mol/L methyl jasmonate, and the pH value is 7.0;
(4) after the rhizoma bletillae tissue culture seedlings are temporarily planted on the substrate for 70-90 days, the seedlings begin to fall down and enter a dormant state, and buds on the pseudobulbs sprout to shoot bud tips; after the dormancy period of the bletilla pseudobulb is finished, the bud tip begins to grow, a first leaf grows, when a third leaf grows, the steps (2) and (3) are repeated, and a strong seedling inducer and a bud inducer are sprayed; after the temporary planting is finished for 6 months, the temporary planting process of the rhizoma bletillae tissue culture seedlings is finished, and the rhizoma bletillae can be outplanted; the seedlings which are out of nursery are dwarf and strong, the leaves are emerald green, the average pseudobulb number is 2.1 per plant, the average pseudobulb size is 1.51 cm, and the average bud number is 4.9 per plant.
Watering for 1 time each morning, applying biogas rich water with lower concentration 20 days after temporary planting, and applying biogas rich water with the same concentration 1 time every 7 days; the spraying of the foliar fertilizer and the inducer is carried out after 4 pm.
Example 3:
a method for promoting strong seedlings and sprouting in pseudobulb tissue culture seedling temporary planting comprises the following steps:
(1) temporarily planting the rhizoma bletillae tissue culture seedlings on a substrate for about 7 days, spraying a foliar fertilizer, and spraying the foliar fertilizer again in 14 days; the foliar fertilizer is 2 per mill potassium dihydrogen phosphate solution;
(2) when the bletilla striata tissue culture seedlings are temporarily planted on the substrate for 21 days, new white roots grow at the root tips of the seedlings, a strong seedling inducer solution is sprayed on leaves, and the strong seedling inducer solution is sprayed for 1 time after 7 days, so that nutrients are favorably gathered to pseudobulb parts, and the expansion of the pseudobulbs is promoted; the strong seedling inducer consists of 2 per mill potassium dihydrogen phosphate solution and 100mg/L paclobutrazol, and the pH value is 7.0;
(3) temporarily planting rhizoma bletillae tissue culture seedlings on a substrate until the interval between the time when the rhizoma bletillae tissue culture seedlings and the last 1 time of spraying of the strong seedling inducer in the step (2) is 10 days, enabling the leaves of the seedlings to be greenish, spraying a bud inducer solution on the leaves, and spraying 1 time after 7 days to promote the bud germination on pseudobulbs; the bud inducer consists of 2 per mill potassium dihydrogen phosphate solution, 30mg/L forchlorfenuron and 20 mu mol/L methyl jasmonate, and the pH value is 7.0;
(4) after the rhizoma bletillae tissue culture seedlings are temporarily planted on the substrate for 70-90 days, the seedlings begin to fall down and enter a dormant state, and buds on the pseudobulbs sprout to shoot bud tips; after the dormancy period of the bletilla pseudobulb is finished, the bud tip begins to grow, each seedling grows more than 2 buds, a first leaf grows, when a third leaf grows, the steps (2) and (3) are repeated, and a strong seedling inducer and a bud inducer are sprayed; after the temporary planting is finished for 6 months, the temporary planting process of the rhizoma bletillae tissue culture seedlings is finished, and the rhizoma bletillae can be outplanted; the seedlings which are out of nursery are dwarf and strong, the average pseudobulb number is 2.3 per plant, the average pseudobulb size is 1.55 cm, and the average bud number is 7.0 per plant.
Watering for 1 time each morning, applying biogas rich water with lower concentration 20 days after temporary planting, and applying biogas rich water with the same concentration 1 time every 7 days; the spraying of the foliar fertilizer and the inducer is carried out after 4 pm.
The following are comparisons of various indicators of the common bletilla striata outplanted seedlings with those of example 3:
by contrast, in the same temporary planting time, the bletilla striata outplanted seedlings subjected to strong seedling and bud promotion treatment have shorter plant heights compared with untreated bletilla striata outplanted seedlings, indexes such as the number of pseudo bulbs, the size of the pseudo bulbs, the number of buds, the root length, the number of roots and the like are all higher than those of untreated bletilla striata outplanted seedlings, wherein the number of the pseudo bulbs and the number of buds closely related to the yield are increased by nearly one time, when the seedlings are planted in a field to the end of the year, 7 buds can have 7 pseudo bulbs, the number of the buds of each pseudo bulb in the next year is 2-4, the number of the buds of each seedling is more than 14, and by analogy, the number of the pseudo bulbs of the bletilla striata tissue culture seedling reaches more than 30 at the end of the third year, and the yield is considerable. Therefore, the bletilla striata seedlings which are strong in seedlings and bud-promoting have wide market prospects, the survival rate of the bletilla striata seedlings transplanted to a field is high, high yield can be achieved within 3 years, the yield is high compared with that of untreated seedlings which are out of the garden for 4 years, the time of 1 year is shortened completely, and the benefit is obvious.
Claims (2)
1. A method for promoting strong seedlings and sprouting in pseudobulb tissue culture seedling temporary planting comprises the following steps:
(1) temporarily planting the rhizoma bletillae tissue culture seedlings on a substrate for about 7 days, spraying a foliar fertilizer, and spraying the foliar fertilizer again in 14 days;
the foliar fertilizer is 2 per mill potassium dihydrogen phosphate solution;
(2) pseudobulb tissue culture seedlings are temporarily planted on a substrate until white new roots grow on the root tips of the seedlings, and a strong seedling inducer solution is sprayed on leaves and is sprayed for 1 time after 7 days, so that nutrients are favorably gathered to pseudobulb parts, and the expansion of the pseudobulbs is promoted;
the strong seedling inducer consists of 2 per mill potassium dihydrogen phosphate solution and 50-200 mg/L paclobutrazol, and the pH value is 7.0;
(3) temporarily planting rhizoma bletillae tissue culture seedlings on a substrate until the interval between the temporary planting of the rhizoma bletillae tissue culture seedlings and the last 1-time spraying of the strong seedling inducer in the step (2) is about 10 days, wherein the leaves of the seedlings are greenish, spraying a bud inducer solution on the leaves, and spraying 1 time after 7 days to promote the bud germination on pseudobulbs;
the bud inducer consists of 2 per mill potassium dihydrogen phosphate solution, 10-50 mg/L forchlorfenuron and 10-50 mu mol/L methyl jasmonate, and the pH value is 7.0;
(4) the rhizoma bletillae tissue culture seedlings are temporarily planted on the substrate until the seedlings are poured successively, the rhizoma bletillae tissue culture seedlings enter a dormant state, and pseudobulbs begin to sprout to emit bud tips; after the dormancy period of the bletilla pseudobulb is finished, the bud tips begin to grow, more than 2 buds grow on each seedling, leaves grow, when the third leaf grows, the steps (2) and (3) are repeated, and a strong seedling inducer and a bud inducer are sprayed; after the temporary planting is finished for 6 months, the temporary planting period of the rhizoma bletillae tissue culture seedlings is finished, and the rhizoma bletillae is outplanted.
2. The method for promoting strong seedlings and sprouting in the process of pseudobulb bletilla striata tissue culture seedlings heeling in according to claim 1, wherein the method comprises the following steps: the strong seedling inducer in the step (2) consists of 2 per mill potassium dihydrogen phosphate solution and 100mg/L paclobutrazol, and the pH value is 7.0;
the bud inducer in the step (3) consists of 2 per mill potassium dihydrogen phosphate solution, 30mg/L forchlorfenuron and 20 mu mol/L methyl jasmonate, and the pH value is 7.0.
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