CN109480139A - A kind of chlorella pyrenoidosa polypeptide anti-ageing drink and preparation method thereof - Google Patents
A kind of chlorella pyrenoidosa polypeptide anti-ageing drink and preparation method thereof Download PDFInfo
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- CN109480139A CN109480139A CN201811553335.0A CN201811553335A CN109480139A CN 109480139 A CN109480139 A CN 109480139A CN 201811553335 A CN201811553335 A CN 201811553335A CN 109480139 A CN109480139 A CN 109480139A
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- 241000195652 Auxenochlorella pyrenoidosa Species 0.000 title abstract 3
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Botany (AREA)
- Non-Alcoholic Beverages (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to technical field of health care food, the preparation methods of specially a kind of chlorella pyrenoidosa polypeptide anti-ageing drink and this kind of anti-ageing drink.Anti-ageing drink of the invention is using chlorella pyrenoidosa extracting solution as one of main component, and with the component compatibilities such as tremella polysaccharides extracting solution, astragalus polyose extracting solution, haw juice and apple juice concentrate, the effect of each component can be given full play to, the mutual cooperative gain of each component, to make anti-ageing drink of the invention that there are good anti-aging effects.Anti-ageing drink of the invention is grouped as by described group centainly matched, not only equilibrium full of nutrition, and sour and sweet palatability, palatable delicious, healthy and safe, be suitable for any age crowd it is edible.
Description
Technical field
The present invention relates to technical field of health care food more particularly to a kind of chlorella pyrenoidosa polypeptide anti-ageing drink, with
And the preparation method of this kind of anti-ageing drink.
Background technique
With being constantly progressive for society, continuous improvement of people's living standards, requirement of the people to daily diet is not only
It is nutrition needed for capable of maintaining, and requires have certain health-care efficacy.Polypeptide is a-amino acid so that peptide bond links together and is formed
Compound, it is also the intermediate product of protein hydrolysis, and the compound as made of two amino acid molecular dehydrating condensations is called
Dipeptides similarly analogizes also tripeptides, tetrapeptide, pentapeptide etc..Usually formed by three or three or more amino acid molecular dehydrating condensations
Compound can be known as polypeptide.For protein, the superiority of small-molecular peptides segment is mainly reflected in: be easier to by
Absorption of human body utilizes;It is active high, its distinctive physiological action can be played under smaller concentration;Compared to single amino acid
Speech, small-molecular peptides also have amino acid without comparable other than with special physiological activity in absorbing path and infiltration rate
Quasi- superiority.Many studies have shown that biologically active peptide has a variety of works such as anti-oxidant, antitumor, antibacterial, decompression, hypoglycemic
With, become biological medicine and health care product exploitation hot spot.Polysaccharide is a kind of long-chain sugar, and a variety of mushroom classes and its mycelia are all rich in polysaccharide
Body, it is extremely beneficial to human immune system, it is used for healthcare field always for many years, and not will cause side effect.However, at present
Shortcoming polypeptide makes the two that can give full play to the health product of its effect in conjunction with polysaccharide in the market.
Summary of the invention
The present invention proposes aiming at the problem that health care product of existing health product technology field shortcoming polypeptide and polysaccharide connected applications
A kind of polypeptide and polysaccharide connected applications, the effect of polypeptide and polysaccharide can be given full play to effect, and it is full of nutrition, safe and healthy, can
The preparation method of the delicious chlorella pyrenoidosa polypeptide anti-ageing drink of mouth and this kind of anti-ageing drink.
To achieve the above object, the present invention uses following technical scheme.
A kind of chlorella pyrenoidosa polypeptide anti-ageing drink in anti-ageing drink described in every 100mL, contains following content
Each component: chlorella pyrenoidosa protein extract 15-25ml, tremella polysaccharides extracting solution 4-8ml, astragalus polyose extracting solution 4-
6ml, haw juice 2-5ml, apple juice concentrate 4-8ml, erythrose 2-4g, citric acid 0.1-0.2g, vitamin C 0.01-0.05g.
Preferably, it in anti-ageing drink described in every 100mL, is grouped as by each group of following content: chlorella pyrenoidosa
It is protein extract 20ml, tremella polysaccharides extracting solution 6ml, astragalus polyose extracting solution 5ml, haw juice 3ml, apple juice concentrate 6ml, red
Moss sugar 3g, citric acid 0.14g, vitamin C 0.05g, surplus are pure water.
A kind of preparation method of chlorella pyrenoidosa polypeptide anti-ageing drink, comprising the following steps: by above-described anti-
In aging beverage the content of each component by chlorella pyrenoidosa protein extract, tremella polysaccharides extracting solution, astragalus polyose extracting solution,
Haw juice, apple juice concentrate, erythrose, citric acid and vitamin C are mixed and stirred for uniformly, and pure water is then added to mixed liquor
Volume be 100mL, then homogeneous and filling and sealing obtain anti-ageing drink followed by through pasteurization.
Preferably, it the preparation method of the chlorella pyrenoidosa protein extract: using chlorella pyrenoidosa as raw material, is added
The pure water that 20 times of chlorella pyrenoidosa weight, using supercritical extract, Extracting temperature is 100-160 DEG C, extracts pressure and is
20MPa, ultrasonic power 300W, extraction time 120min.
Preferably, the preparation method of the tremella polysaccharides extracting solution: tremella is crushed, and adds 6 times of tremella weight of pure water,
2h is extracted at a temperature of 70-90 DEG C, is extracted twice, is merged extracting solution twice and filtering, obtain tremella extract.
Preferably, the preparation method of the astragalus polyose extracting solution: Radix Astragali is crushed, and adds 10 times of astragalus weight pure
Water extracts 2h at a temperature of 70-90 DEG C, extracts twice, merges extracting solution twice and filtering, obtain astragalus polyose extracting solution.
Preferably, after hawthorn is enucleated, hawthorn the preparation method of the haw juice: is placed in the aqueous solution of 2 times of its weight
In, it squeezes the juice at 25 DEG C, haw juice is obtained after filtering.
Preferably, the preparation method of the apple juice concentrate: after apple is enucleated, being placed in the aqueous solution of 3 times of its weight,
It squeezes the juice at 25 DEG C, cider is obtained after filtering.
Compared with prior art, the beneficial effects of the present invention are:
Contain chlorella pyrenoidosa protein extract, chlorella pyrenoidosa polypeptide therein in anti-ageing drink of the invention
It is a kind of high-quality protein raw material, containing the several functions protein such as growth factor and small-molecular peptides, good with palatability,
The features such as protein content height, amino acid balance, absorption rate are high, added value is high, can be widely applied to food, health food
And field of medicaments.
Contain tremella polysaccharides extracting solution in anti-ageing drink of the invention, tremella polysaccharides therein are that a kind of acidity is miscellaneous more
Sugar, backbone structure are made of the mannosan of α-(1 → 3) glucosides key connection, and branch is made of glucuronic acid and xylose,
With immunoregulation capability is improved, the effect of cellular immunity and humoral immunity is improved, auxiliary inhibits the effect of tumour, can improve swollen
The content of phosphatide adenosine in oncocyte changes tumour cell to influence the metabolism of tumour cell amplifying nucleic acid and protein
Splitting characteristic converts tumour cell to normal direction, also has and delays senescence, adjusts blood lipid, pre- preventing thrombosis, protection gastric mucosa
The effects of.Tremella polysaccharides are significant to aging, immunoregulation effect, can be widely applied to food, health food and field of medicaments.
Contain astragalus polyose extracting solution in anti-ageing drink of the invention, astragalus polyose therein is by hexuronic acid, Portugal
The composition such as grape sugar, fructose, rhamnose, arabinose, galacturonic acid and glucuronic acid, can be used as immunopotentiating agent or adjusting
Agent, while there is antiviral and antitumor, anti-aging, anti-radiation, resisting stress, anti-oxidant, antifatigue, liver protection, adjust immune function
The effects of with stimulation interferon formation, have been widely used for food, health food and field of medicaments.
Contain haw juice in anti-ageing drink of the invention, hawthorn is one of traditional common Chinese medicine and familiar
Food digesting stomach fortifying food, modern scientific research show hawthorn containing hawthorn acid, flavonoids, carbohydrate, glucoside, tannin, protein, calcium,
Phosphorus, iron and multivitamin ingredient, can promote lipolysis, help digest, and there are also antibacterial, reducing blood lipid, heart tonifying, contraction uterus etc.
Effect.More strikingly, there are also good anticancer functions for hawthorn.Research in recent years discovery, hawthorn extract can block Asia
The synthesis of nitramine plays a significant role in terms of preventing and treating alimentary tract cancer, and hawthorn has the mutagenesis of aflatoxins
Significantly inhibit effect.Since hawthorn can help digestion except product, stagnation resolvation are dispelled growth that is stagnant, and can inhibiting cancer cell, thus it is suitable for a variety of
The prevention and treatment of solid tumor.The particularly suitable loss of appetite of hawthorn, dyspeptic patient, can play preferable auxiliary therapeutic action.
Contain cider in anti-ageing drink of the invention, cider can accelerate intestines peristalsis, improve physical function, supplement
Nutrition reduces the probability of heart disease and artery sclerosis, cleans alimentary canal, promotes food digestion.Cider is a kind of powerful clear
It is clean dose, critically important to health, heart disease can be prevented, reduce LDL value and cholesterol level.When LDL is aoxidized or in blood
Deteriorate, blood platelet will initially form coronary artery, artery is caused to thicken, and cause artery sclerosis.It is a large amount of because containing in cider
Potassium, since cider also can reduce arthritis, rheumatism symptom, to older people, can especially close first drinking cider
Section inflammation, rheumatic sufferers.The also cleanable liver of cider, kidney.In addition, cider can also still low calories drink, persistency reference may be used also
To reduce liver or kidney trouble.Insoluble fibre is a kind of substance for preventing accumulation of cholesterol, is present in arterial blood tube wall, can
The probability of heart disease and artery sclerosis is reduced, the embeddable enteron aisle of insoluble fibre, then and water cleans alimentary canal together, promotes food
Object digestion.
Anti-ageing drink of the invention is extracted using chlorella pyrenoidosa extracting solution as one of main component with tremella polysaccharides
The component compatibilities such as liquid, astragalus polyose extracting solution, haw juice and apple juice concentrate, the effect of each component can be given full play to, each component
Mutual cooperative gain, to make anti-ageing drink of the invention that there are good anti-aging effects.Anti-ageing drink of the invention
It is grouped as by described group centainly matched, not only equilibrium full of nutrition, and sour and sweet palatability, palatable delicious, healthy and safe, is suitable for appointing
The crowd at what age is edible.
Detailed description of the invention
Fig. 1 is the survival rate cylindricality of nematode with culture medium culture nematode 10 days of the anti-ageing drink prepared containing embodiment 2
Figure;
Fig. 2 is the survival rate cylindricality of nematode with culture medium culture nematode 15 days of the anti-ageing drink prepared containing embodiment 2
Figure;
Fig. 3 is the survival rate cylindricality of nematode with culture medium culture nematode 20 days of the anti-ageing drink prepared containing embodiment 2
Figure.
Specific embodiment
To facilitate the understanding of the present invention, below to invention is more fully described.But the present invention can be with many
Different form is realized, however it is not limited to embodiment described herein.On the contrary, purpose of providing these embodiments is in order to
It is more thorough and comprehensive to the understanding of present disclosure.For those skilled in the art, by reading this explanation
Book disclosure, feature of the invention, beneficial effect and advantage will become obvious.
Embodiment 1-5
Embodiment 1-5 each provides a kind of chlorella pyrenoidosa polypeptide anti-ageing drink and this kind of pyrenoids bead
The preparation method of algae polypeptide anti-ageing drink.The content of each component is such as in every 100mL chlorella pyrenoidosa polypeptide anti-ageing drink
Shown in following table.
Embodiment | 1 | 2 | 3 | 4 | 5 |
Chlorella pyrenoidosa protein extract/ml | 15 | 20 | 25 | 20 | 20 |
Tremella polysaccharides extracting solution/ml | 8 | 6 | 4 | 5 | 7 |
Astragalus polyose extracting solution/ml | 6 | 5 | 4 | 5 | 5 |
Haw juice/ml | 5 | 3 | 2 | 5 | 5 |
Apple juice concentrate/ml | 4 | 6 | 8 | 7 | 8 |
Erythrose/g | 2 | 3 | 4 | 3 | 3 |
Citric acid/g | 0.1 | 0.14 | 0.2 | 0.15 | 0.15 |
Vitamin C/g | 0.05 | 0.05 | 0.03 | 0.02 | 0.01 |
Pure water | Surplus | Surplus | Surplus | Surplus | Surplus |
The preparation method of chlorella pyrenoidosa polypeptide anti-ageing drink, includes the following steps S1-S3:
S1 stock:
The preparation of chlorella pyrenoidosa protein extract: using chlorella pyrenoidosa as raw material, chlorella pyrenoidosa weight is added
The pure water of 20 times of amount, using supercritical extract, Extracting temperature is 150 DEG C and (mainly controls Extracting temperature at 100-160 DEG C
It is interior), extraction pressure is 20MPa, ultrasonic power 300W, extraction time 120min;It is spare.
The preparation of tremella polysaccharides extracting solution: tremella is crushed, and adds 6 times of tremella weight of pure water, in 70-90 DEG C of temperature
Lower extraction 2h is extracted twice, is merged extracting solution twice and filtering, obtain tremella extract;It is spare.
The preparation of astragalus polyose extracting solution: Radix Astragali is crushed, and adds 10 times of astragalus weight of pure water, in 70-90 DEG C of temperature
Degree is lower to extract 2h, extracts twice, merges extracting solution twice and filtering, obtain astragalus polyose extracting solution;It is spare.
The preparation of haw juice: after hawthorn is enucleated, hawthorn being placed in the aqueous solution of 2 times of its weight, squeezed the juice at 25 DEG C, mistake
Haw juice is obtained after filter;It is spare.
The preparation of apple juice concentrate: after apple is enucleated, being placed in the aqueous solution of 3 times of its weight, squeeze the juice at 25 DEG C, filtering
Cider is obtained afterwards;It is spare.
S2 mixing:
Chlorella pyrenoidosa protein extract, tremella polysaccharides extracting solution, astragalus polyose is weighed respectively by consumption proportion to extract
Liquid, haw juice, apple juice concentrate, erythrose, citric acid and vitamin C, and these components are stirred evenly;Then it is added pure
Water produces predetermined volume, and homogeneous to the volume of mixed liquor.
S3 is canned and sterilizes:
Mixed liquor made from step S2 is fitted into tank, sterilization treatment is carried out using pasteurization, obtains anti-ageing drink.
The measurement of anti-aging effects
Experiment test one: by the raising of hermaphroditic nematode on the nematode standard medium of coating Escherichia coli, 20 DEG C are fallen
Set culture.The 4th day after synchronization, picking nematode of the same size is put into no medicine culture medium and containing different medicine for nematode biometrics
Culture in the culture medium of amount (medicine is anti-ageing drink prepared by embodiment 2).It is divided into control group (the not culture medium of drug containing), low dose
Amount group (content of dispersion is 20mg/mL in culture medium), middle dose group (content of dispersion is 40mg/mL in culture medium) and high dose group (training
Supporting content of dispersion in base is 60mg/mL).Be denoted as nematode life with the grouping same day and originate the 0th day, with insect-taking device end gently visit polypide without
Reaction is denoted as the termination of nematode life, is denoted as nematode lifetime from life starting to the time is terminated.In nematode lifetime, per for 24 hours
Nematode on each plate is transferred in identical new plate, the survival rate of the 10th day each group nematode is recorded.
The survival rate of 10th day each group nematode is as shown in Figure 1, the survival rate of the nematode of control group is 70.05%, low dosage
The survival rate of the nematode of group is 74.38%, and the survival rate of the nematode of middle dose group is 78.12%, and the nematode of high dose group is deposited
Motility rate is 76.43%.
It carries out experiment test two and experiment test three respectively in the same way, measures the 15th day and the 20th day each group respectively
The survival rate of nematode.
The survival rate of 15th day each group nematode is as shown in Fig. 2, the survival rate of the nematode of control group is 36.15%, low dosage
The survival rate of the nematode of group is 42.03%, and the survival rate of the nematode of middle dose group is 47.71%, and the nematode of high dose group is deposited
Motility rate is 49.00%.
The survival rate of 20th day each group nematode is as shown in figure 3, the survival rate of the nematode of control group is 6.25%, low dose group
The survival rate of nematode be 5.83%, the survival rate of the nematode of middle dose group is 9.7%, and the survival rate of the nematode of high dose group is
9.17%.
With the anti-of the method difference testing example 1 and embodiment 3-5 preparation of above-mentioned experiment test one and experiment test two
The senile-resistant efficacy of aging beverage.With the nematode of the culture medium culture of the anti-ageing drink prepared containing embodiment, the 10th day and
Survival rate of 15 days survival rates than the nematode with the culture medium culture without anti-ageing drink is high.
It is described above that technology contents of the invention are only further illustrated with embodiment, in order to which reader is easier to understand,
But embodiments of the present invention are not represented and are only limitted to this, any technology done according to the present invention extends or recreation, is sent out by this
Bright protection.
Claims (8)
1. a kind of chlorella pyrenoidosa polypeptide anti-ageing drink, which is characterized in that in anti-ageing drink described in every 100mL, contain
There is each component of following content:
Chlorella pyrenoidosa protein extract 15-25ml, tremella polysaccharides extracting solution 4-8ml, astragalus polyose extracting solution 4-6ml, mountain
Short, bristly hair or beard juice 2-5ml, apple juice concentrate 4-8ml, erythrose 2-4g, citric acid 0.1-0.2g, vitamin C 0.01-0.05g.
2. chlorella pyrenoidosa polypeptide anti-ageing drink according to claim 1, which is characterized in that described in every 100mL
In anti-ageing drink, it is grouped as by each group of following content:
Chlorella pyrenoidosa protein extract 20ml, tremella polysaccharides extracting solution 6ml, astragalus polyose extracting solution 5ml, haw juice 3ml,
Apple juice concentrate 6ml, erythrose 3g, citric acid 0.14g, vitamin C 0.05g, surplus are pure water.
3. a kind of preparation method of chlorella pyrenoidosa polypeptide anti-ageing drink, which comprises the following steps: press right
It is required that in chlorella pyrenoidosa polypeptide anti-ageing drink described in 1 each component content by chlorella pyrenoidosa protein extract,
Tremella polysaccharides extracting solution, astragalus polyose extracting solution, haw juice, apple juice concentrate, erythrose, citric acid and vitamin C mixing are simultaneously
It stirs evenly, the volume that pure water is then added to mixed liquor is 100mL, then homogeneous and filling and sealing, followed by through Pasteur
Sterilizing, obtains anti-ageing drink.
4. the preparation method of chlorella pyrenoidosa polypeptide anti-ageing drink according to claim 3, which is characterized in that described
The preparation method of chlorella pyrenoidosa protein extract: using chlorella pyrenoidosa as raw material, chlorella pyrenoidosa weight 20 is added
Times pure water, using supercritical extract, Extracting temperature is 100-160 DEG C, and extractions pressure is 20MPa, ultrasonic power 300W,
Extraction time 120min.
5. the preparation method of chlorella pyrenoidosa polypeptide anti-ageing drink according to claim 3, which is characterized in that described
The preparation method of tremella polysaccharides extracting solution: tremella is crushed, and is added 6 times of tremella weight of pure water, is mentioned at a temperature of 70-90 DEG C
2h is taken, is extracted twice, is merged extracting solution twice and filtering, obtain tremella extract.
6. the preparation method of chlorella pyrenoidosa polypeptide anti-ageing drink according to claim 3, which is characterized in that described
The preparation method of astragalus polyose extracting solution: Radix Astragali is crushed, and adds 10 times of astragalus weight of pure water, at a temperature of 70-90 DEG C
2h is extracted, is extracted twice, is merged extracting solution twice and filtering, obtain astragalus polyose extracting solution.
7. the preparation method of chlorella pyrenoidosa polypeptide anti-ageing drink according to claim 3, which is characterized in that described
The preparation method of haw juice: after hawthorn is enucleated, hawthorn is placed in the aqueous solution of 2 times of its weight, is squeezed the juice at 25 DEG C, after filtering
Obtain haw juice.
8. the preparation method of chlorella pyrenoidosa polypeptide anti-ageing drink according to claim 3, which is characterized in that described
The preparation method of apple juice concentrate: after apple is enucleated, being placed in the aqueous solution of 3 times of its weight, squeeze the juice at 25 DEG C, after filtering
Cider.
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