CN109479834A - A kind of device and method for causing insect traumatic brain injury that dynamics is controllable - Google Patents
A kind of device and method for causing insect traumatic brain injury that dynamics is controllable Download PDFInfo
- Publication number
- CN109479834A CN109479834A CN201811561124.1A CN201811561124A CN109479834A CN 109479834 A CN109479834 A CN 109479834A CN 201811561124 A CN201811561124 A CN 201811561124A CN 109479834 A CN109479834 A CN 109479834A
- Authority
- CN
- China
- Prior art keywords
- insect
- fixed plate
- conduit
- hole
- preferable
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000238631 Hexapoda Species 0.000 title claims abstract description 163
- 208000030886 Traumatic Brain injury Diseases 0.000 title claims abstract description 18
- 230000009529 traumatic brain injury Effects 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 9
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 14
- 229910000831 Steel Inorganic materials 0.000 claims description 28
- 239000010959 steel Substances 0.000 claims description 28
- 239000011521 glass Substances 0.000 claims description 11
- 238000002474 experimental method Methods 0.000 claims description 7
- 239000000945 filler Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 229920003023 plastic Polymers 0.000 claims description 5
- 206010019196 Head injury Diseases 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 230000003187 abdominal effect Effects 0.000 claims description 3
- 230000001154 acute effect Effects 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 239000004033 plastic Substances 0.000 claims description 3
- 239000004568 cement Substances 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 238000009827 uniform distribution Methods 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 238000002360 preparation method Methods 0.000 abstract description 2
- 230000035939 shock Effects 0.000 description 8
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 7
- 230000006378 damage Effects 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 230000035882 stress Effects 0.000 description 5
- 238000009826 distribution Methods 0.000 description 4
- 208000022306 Cerebral injury Diseases 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 238000010171 animal model Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 239000003292 glue Substances 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000003252 repetitive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 206010002091 Anaesthesia Diseases 0.000 description 1
- 241000208340 Araliaceae Species 0.000 description 1
- 208000013883 Blast injury Diseases 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical group [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000037005 anaesthesia Effects 0.000 description 1
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007321 biological mechanism Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 230000001149 cognitive effect Effects 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000001054 cortical effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000009527 percussion Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 208000020431 spinal cord injury Diseases 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
Abstract
A kind of device and method for causing insect traumatic brain injury that dynamics is controllable, described device includes: pedestal, upright bar, fixing piece, conduit, weight, crash panel and insect fixed plate；Wherein the upright bar is vertically arranged on the base；The fixing piece is horizontally set in the upright bar, for fixing the conduit；The crash panel is located at below the conduit；The weight is placed in the conduit when in use and hits the crash panel when falling；The insect fixed plate is located at the lower section of the crash panel, the insect fixed plate has one or more insect fixation holes and multiple spring mounting holes, support spring is provided in the spring mounting hole, the top of the support spring is higher than the insect fixed plate, and the crash panel is arranged in the support spring.The device of the invention structure is simple, cheap, is easy production preparation, realizes the accurate control to insect head impact dynamics and impact site.
Description
Technical field
The present invention relates to biology experimental installation field, specially a kind of dynamics it is controllable cause insect traumatic brain injury
Device and method.
Background technique
Traumatic brain injury (traumatic brain injury, TBI) is that a global threat human brain is strong
The important diseases of health.According to statistics, global TBI average originating rate is 2 ‰ (2,00/,100,000), and average mortality is 0.2 ‰ (20/10
Ten thousand), that a situation arises is different by every country TBI, and incidence range is (91-430)/100,000 (Reilly and
Bullock, Current evaluation of TBI epidemiology in an ageing society with
Improved preventative measures.Traumatic brain and spinal cord injury:
Challenges and developments, Cambridge University Press, Cambridge, 2005).Severe
Cerebral injury will cause death, and the obstacle in terms of slight cerebral injury will cause Patients ' Cognitive, spirit and behavioral function
(Zaloshnja et al., Prevalence of long-term disability from traumatic brain
injury in the civilian population of the United States.The Journal of head
Trauma rehabilitation 23:394-400,2008).At present about traumatic brain injury in body research model,
Causing the experimental provision of laboratory rodent rat and mouse traumatic brain injury includes hydraulic shock model (Fluid
Percussion model), controlling cortex impact-model (Controlled cortical impact injury
Model), (Estrada- such as weight drop model (Weight drop model) and bang model (Blast Injury model)
Rojo et al., Models used in the study of traumatic brain injury.Reviews in the
Neurosciences 29:139-149,2018).
On the other hand, insects experimental animal drosophila as biological study classical model, research TBI molecule and
There is unique advantage in terms of cell biological mechanisms.At present in the field there are mainly two types of used experimental provisions: one
It is spring slam device (Katzenberger et al., A Drosophila model of closed head
Traumatic brain injury.Proc Natl Acad Sci U S A 110:E4152-4159,2013), the second is from
Scheming device (Barekat et al., Using Drosophila as an integrated model to study mild
Repetitive traumatic brain injury.Scientific Reports 6,2016).
The above two device for causing drosophila cerebral injury is primarily present following problems: first, to the impact force of experimental animal
Spend it is uncontrollable, have randomness；Second, it is uncontrollable to the impact site of experimental animal, there is randomness；Third, in addition to head
Except shock, the shock and damage at other positions also will cause, so that the experimental analysis to the later period affects greatly.
Summary of the invention
The object of the present invention is to provide a kind of production simply, and low in cost, dynamics is controllable, and site of action is accurate, Neng Goutong
More insect impact sites of Shi Zaocheng are consistent and the experimental provision of the consistent traumatic brain injury of degree of injury.
The purpose of the present invention is what is be achieved through the following technical solutions:
On the one hand, the present invention provides a kind of device for causing insect traumatic brain injury that dynamics is controllable, comprising: pedestal,
Upright bar, fixing piece, conduit, weight, crash panel and insect fixed plate；
Wherein the upright bar is vertically arranged on the base；
The fixing piece is horizontally set in the upright bar, for fixing the conduit；
The crash panel is located at below the conduit；
The weight is placed in the conduit when in use and hits the crash panel when falling；
The insect fixed plate is located at the lower section of the crash panel, and the insect fixed plate has one or more insects solid
Determine hole and multiple spring mounting holes, support spring is provided in the spring mounting hole, the top of the support spring is higher than institute
Insect fixed plate is stated, the crash panel is arranged in the support spring.
In some embodiments, the conduit is made of clear material, preferably glass, transparent plastic (such as PVC) etc.,
Preferably, scale is labeled on the inside or outside of the conduit, it is preferable that the conduit is parallel with the upright bar or stands with described
Bar forms an inclination angle.
In some embodiments, the weight is steel ball, it is preferable that the diameter of the steel ball is smaller than the internal diameter of the conduit
0-2 millimeters, it is highly preferred that being provided with drawstring on the weight.
In some embodiments, the fixing piece is clip, it is preferable that the quantity of the fixing piece is one or more.
In some embodiments, the thickness of the insect fixed plate is long more than or equal to the body of insect, and the insect is fixed
The shape of plate is disc, square or rectangular.
In some embodiments, the insect fixation hole is through-hole and including upper opening and under shed, and upper opening
Aperture be less than under shed aperture, the insect fixation hole is preferably bellmouth, it is highly preferred that the Zhou Changwei of the upper opening
1-1.5 times of insect head circumference, 1.5-2 times of the Zhou Changwei insect abdominal circumference maximum of under shed.
In some embodiments, the axis of the insect fixation hole it is vertical with the insect fixed plate or with the insect
Fixed plate is in an acute angle.
In some embodiments, the insect fixation hole includes the circular hole for running through the insect fixed plate, is arranged described
Conical pipe in circular hole and the filler between the circular hole and the conical pipe, it is preferable that the filler is silicon
Glue or glass cement.
In some embodiments, the surrounding that the spring mounting hole is located at the insect fixed plate is uniformly distributed, it is preferable that
The quantity of the spring mounting hole is 3-8.
In some embodiments, the spring mounting hole is through-hole or groove, it is preferable that the spring mounting hole includes cone
Shape hole and cylindrical hole below, the aperture of the upper opening of the bellmouth are greater than the aperture of the under shed of the bellmouth, institute
The under shed for stating bellmouth is connected with the cylindrical hole, it is preferable that the aperture of the upper opening of the bellmouth is the bellmouth
1.5-2.5 times of aperture of under shed, more preferably 2 times, the aperture of the under shed of the bellmouth and the cylindrical hole
Aperture is identical, and the height of the bellmouth is 0.5-2 times of the height of the cylindrical hole, the height of the more preferable bellmouth and
The height of the cylindrical hole is identical.In some embodiments, described device further includes gasket, and it is solid that the gasket is located at the insect
The lower section of fixed board, it is preferable that when the spring mounting hole is through-hole, the gasket is used to support the support spring, the pad
The shape of piece is corresponding with the distribution of the spring mounting hole.
In some embodiments, the shape and size of the crash panel are identical as the shape and size of the insect fixed plate
It is or different, it is preferable that the material of the crash panel is selected from plastics (preferably PVC), metal, timber or glass, it is preferable that institute
The shape for stating crash panel is disc, square or rectangular.
In some embodiments, described device further includes the insect collection vessel below the insect fixed plate, excellent
Selection of land, the insect collection vessel are plate.
On the other hand, the present invention also provides a kind of methods for carrying out head injury experiment using described device, comprising: by elder brother
Worm is fixed in the insect fixation hole, and its head is made to be completely exposed to the surface of the insect fixed plate；By the weight
It is added in the conduit and is fallen on the crash panel under predetermined position, make the head of insect described in the impaction plates
Portion.
In some embodiments, using described device carry out head injury experiment method specifically includes the following steps:
Pedestal is placed on horizontal experimental bench, pedestal is fixed in upright bar, and clip is fixed in upright bar, is then pressed from both sides with clip
It stays and falls conduit, adjust clip and whereabouts conduit, the conduit that causes to fall and break are maintained a certain distance perpendicular to experimental bench, and with pedestal,
Insect collection vessel is placed in immediately below the conduit of whereabouts.After insect is anaesthetized, insect wing is gently picked up with tweezers, it is solid from insect
Insect is carefully filled in fixed board bellmouth macropore, stretch out its head from bellmouth aperture and is completely exposed to insect fixed plate
Surface.Insect fixed plate equipped with insect is placed in the surface of insect collection vessel, while be located at immediately below the conduit of whereabouts, so
Crash panel, gently for frame right above insect fixed plate, the support spring between insect fixed plate and crash panel can guarantee the two afterwards
Between distance away so that insect head at this time is not hit or is oppressed.Steel ball, which is put into whereabouts conduit, to be made
It is in certain altitude, and then freely falling body strikes the centre of crash panel, generates downward pressure after crash panel stress and hits
Insect head.It is fallen in insect collection vessel after insect stress from insect fixed plate, can be used for subsequent experimental.Insect head
The size of stress can realize that the weight of steel ball is bigger, and the height of whereabouts is got over by adjusting weight and the height of drop of steel ball
Height, the impact force that crash panel is subject to is bigger, and the impact force that insect head is subject to is bigger, damages more serious.
Compared with prior art, the invention has the following advantages that
1, apparatus structure is simple, cheap, is easy production preparation, realizes to insect head impact dynamics and shock
The control at position.
2, the device is using the fixed insect of unique insect fixed plate, it can be achieved that more insects are simultaneously by shock degree phase
Same power, thus the damage for causing insect head degree almost the same.
3, the device is hit using steel ball, can accurately be measured steel ball weight and height of drop, be passed through different lower drop heights
Degree realizes different degrees of collision injury.
4, the device has whereabouts conduit, and whereabouts conduit indicates scale, the quantization of steel ball drop height not only may be implemented,
It more can guarantee that the motion profile of steel ball in experiment is steadily controllable, be unlikely to generate deviation.
5 devices have whereabouts conduit, and whereabouts catheter center, face crash panel center, position can guarantee reality in this way
It tests identical to the shock dynamics of every insect when middle steel ball shock crash panel.
6, the size of the device is related to crash panel, steel ball, whereabouts conduit, clip, upright bar and pedestal can be according to reality
Need flexible modulation.
7, the size and hardness of the size of insect fixed plate in the device, thickness, pore size and spring can be according to need
It is adjusted production.
8, the key of fixed insect is under the premise of fixed insect, can to prevent insect from climbing from hole using bellmouth
Out.
Detailed description of the invention
Fig. 1 is the overall schematic of experimental provision of the present invention.
Fig. 2 is the schematic diagram of insect fixed plate in the embodiment of the present invention.
Fig. 3 is the schematic diagram of the spring mounting hole in the embodiment of the present invention.
Fig. 4 is the schematic diagram of spring mounting hole in another embodiment of the present invention.
Fig. 5 is the schematic diagram of tapered openings in the embodiment of the present invention.
Fig. 6 is schematic diagram of the angle less than 90 degree between tapered openings and insect fixed plate in the embodiment of the present invention.
Fig. 7 is the schematic diagram of tapered openings in another embodiment of the present invention.
Description of symbols:
1- pedestal 2- upright bar 3- clip
4- conduit 5- steel ball 6- crash panel
7- insect fixed plate
7.1- spring mounting hole 7.2- support spring 7.3- tapered openings
8- insect collection vessel 9- gasket 10- insect
Specific embodiment
To make the objectives, technical solutions, and advantages of the present invention clearer, below in conjunction with specific embodiment, and reference
Attached drawing, the present invention is described in further detail.
Embodiment one
The experimental provision for causing insect traumatic brain injury of the present embodiment, as shown in Figure 1, including pedestal 1, upright bar 2, folder
Son 3, whereabouts conduit 4, steel ball 5, crash panel 6, insect fixed plate 7, insect collection vessel 8 and gasket 9.Pedestal 1 is placed in experimental bench
On face, upright bar 2 is vertically fixed on pedestal 1, and clip 3 is horizontally fixed in upright bar 2, and whereabouts conduit 4 is fixed on clip 3, is hit
The top that plate 6 is placed in pedestal 1 is hit, and is in the underface of whereabouts conduit 4, the center of whereabouts conduit 4 should be directed at crash panel 6
Center, insect fixed plate 7 is located at the underface of crash panel 6, insect collection vessel 8 be located at insect fixed plate 7 just under
Side.
Pedestal 1 is rectangle, square or disc, is placed on experiment table top, plays support and steady to whole device
Fixed effect.
Upright bar 2 is perpendicularly fixed on pedestal 1 by screw hole and screw.One of clip 3 is perpendicularly fixed at vertical by screw
On bar 2, other end vertical folder, which is stayed, falls conduit 4.In order to guarantee the firm of whereabouts conduit, need to be clamped down respectively with two clips 3
Fall the different location of conduit.Whereabouts conduit 4 should form parallel relation with upright bar 2 at this time, and form vertical relation with pedestal 1.
Material that is transparent and having certain degree of hardness, such as glass, PVC can be selected in whereabouts conduit 4, and inside on whereabouts conduit 4
Or outside can mark scale.
Have drawstring on steel ball 5, convenient for take out steel ball or be lifted up steel ball to certain altitude carry out repetitive operation, and under
The internal diameter of the diameter and whereabouts conduit 4 that fall steel ball 5 should substantially quite.Preferably, the diameter of steel ball 5 is less than the interior of whereabouts conduit 4
0-2 millimeters of diameter.
Crash panel 6 is the shapes such as disc, square, rectangle, is lighter weight, thickness is smaller, has certain degree of hardness
Plate.Hard material such as plastics (such as PVC), metal, timber or glass production can be used in crash panel 6.
Insect fixed plate 7 can be the shapes such as disc, square, rectangle, the shape of insect fixed plate 7 can with hit
The shape for hitting plate 6 is identical or different, and the size of insect fixed plate 7 can be identical or different with crash panel 6, for example, working as crash panel 6
Shape be disc when, the shape of insect fixed plate 7 can be disc, or the shapes such as square, rectangle, such as
The shape of fruit insect fixed plate 7 is disc, and diameter can be identical as the diameter of crash panel 6, can also be more straight than crash panel 6
Diameter is slightly larger or smaller, as long as can guarantee that crash panel 6 can strike all insects in insect fixed plate 7.Insect is fixed
The thickness of plate 7 can be selected according to the size of variety classes insect, and under normal circumstances, the thickness of insect fixed plate 7 should compare
The body for the insect tested is long slightly long.By taking drosophila as an example, drosophila body is grown at 0.3 centimetre or so, and 7 thickness of insect fixed plate can be
It is selected between 0.4-0.6 centimetres.
As Figure 2-3, the periphery of insect fixed plate 7 is arranged there are four spring mounting hole 7.1 in spring mounting hole 7.1
There is support spring 7.2,7.2 height of support spring is higher by insect fixed plate 7, and such crash panel 6 is placed in 7 top of insect fixed plate
It is maintained a certain distance Shi Keyu, this distance can be adjusted according to the size on different insects head, under normal circumstances,
This distance should be more slightly larger than the height on insect head (by taking drosophila as an example, this distance is between 1-2 millimeters).Spring mounting hole
7.1 and support spring 7.2 be at least respectively 3, preferably 8, and be uniformly distributed in insect fixed plate 7, such as when insect is fixed
When plate 7 is a disk shape, spring mounting hole 7.1 is consistent apart from edge length, and angle is impartial between each hole and the line in the center of circle.
The quantity of support spring 7.2 is consistent with the quantity of spring mounting hole 7.1, the related ginseng such as height, hardness of different support springs 7.2
Number is consistent.
As shown in figure 3, spring mounting hole 7.1 includes bellmouth 7.1.1 and cylindrical hole 7.1.2, spring mounting hole 7.1 can be with
For through insect fixed plate 7.Cylindrical hole 7.1.2 internal diameter is identical as 7.2 outer diameter of support spring, for fixing support spring 7.2.Cone
Shape hole 7.1.1 avoids being compressed to and can not compressing when support spring 7.2 for accommodating the support spring 7.2 after being moved down by compression
When, highly it is higher than the surface of insect fixed plate 7, influences shock of the crash panel 6 to insect.Bellmouth 7.1.1 and cylindrical hole 7.1.2
Size can be according to height and hardness of spring, and convenience of operation etc. be selected.Preferably, bellmouth 7.1.1
Height is identical as the height of cylindrical hole 7.1.2, and the lower aperture of bellmouth 7.1.1 is identical as the pore size of cylindrical hole 7.1.2, cone
The upper aperture of shape hole 7.1.1 is 2 times of lower aperture.
As shown in Figure 2 and Figure 5, there are some tapered openings 7.3 in the middle section of insect fixed plate 7, can be used for fixing insect
10, so that it is head-up exposed 7 surface of insect fixed plate.7.3 top aperture of tapered openings is smaller, and lower section aperture is larger, so that elder brother
Worm, which is placed in one, to be not easy to climb out of.The size of tapered openings 7.3 can be adjusted according to the size of variety classes insect, for example, its
Top aperture perimeter can be equivalent to 1-1.5 times of institute's experimental insect head circumference, and lower section aperture perimeter can be equivalent to insect
1.5-2 times of abdominal circumference maximum.Depending on the quantity of tapered openings 7.3 is as needed, position should be evenly distributed in insect fixed plate 7
On.
The quantity of tapered openings 7.3, generally in dozens of or so, can be convenient for a series of subsequent behaviour depending on experiment
Make.The position of tapered openings 7.3 is uniformly distributed in insect fixed plate 7.
Insect collection vessel 8 is plate shape, is located at immediately below insect fixed plate 7, is fallen from insect fixed plate 7 for collecting
The insect 10 fallen.
It is also provided with gasket 9 between insect fixed plate 7 and insect collection vessel 8, is used to support spring mounting hole 7.1
Interior support spring 7.2, prevents it from falling from spring mounting hole 7.2.Gasket 9 can cover the region of spring mounting hole 7.1,
The region of the upper conical aperture 7.3 of insect fixed plate 7 is not covered simultaneously.That is, the shape of gasket 9 depends on spring mounting hole 7.1
Distribution situation, for example, the shape of gasket 9 is a circle when multiple spring mounting holes 7.1 are in insect 7 rounded distribution of fixed plate
Ring, when multiple spring mounting holes 7.1 are in insect 7 square distribution of fixed plate, the shape of gasket 9 is a square loop, this
Place is not listed one by one.
Insect fixed plate 7 is placed in 8 top of insect collection vessel, crash panel 6 is put in 7 top of insect fixed plate, then sets
In 4 lower section of whereabouts conduit, the height of whereabouts conduit 4, a little higher than 6 certain distance of crash panel in the bottom for the conduit 4 that causes to fall and break are adjusted.Together
When mark scale on whereabouts conduit 4.
Crash panel 6 and insect fixed plate 7 are taken out below whereabouts conduit 4.By the insect 10 of anesthesia from tapered openings 7.3
It is gently filled in the large hole of bottom, head above tapered openings 7.3 in smaller hole from stretching out and be completely exposed to insect fixed plate 7
Surface.Insect fixed plate 7 equipped with insect 10 is placed in immediately below whereabouts conduit 4, then crash panel 6, gently frame is consolidated in insect
Right above fixed board 7, the support spring between insect fixed plate 7 and crash panel 6 be can guarantee between the two distance away, be made
It is not hit or is oppressed in the head for obtaining insect 10 at this time.
Steel ball 5 is taken out, 5 diameter of steel ball and 4 internal diameter of whereabouts conduit are substantially suitable or slightly smaller, lift the drawstring on steel ball 5, will
Steel ball 5 is placed in whereabouts conduit 4.It according to the scale of whereabouts conduit 4, adjusts steel ball 5 and arrives certain altitude, unclamp drawstring, steel ball 5 is certainly
By generating impact force on falling bodies to crash panel 6 and to it, downward pressure is generated after 6 stress of crash panel and hits 10 head of insect.Elder brother
The size of 10 head stress of worm can realize that the weight of steel ball 5 is bigger by the weight and the height of whereabouts for adjusting steel ball 5,
The height of whereabouts is higher, and the impact force that crash panel 6 is subject to is bigger, and the impact force that 10 head of insect is subject to is bigger, and damage is got over
Seriously.
Embodiment two
As shown in figure 4, spring mounting hole 7.1 includes bellmouth 7.1.1 and cylindrical hole 7.1.2, spring mounting hole 7.1 is not passed through
Wear insect fixed plate 7, but a trench structure.This structure can make support spring 7.2 be directly fixed on spring mounting hole
Among 7.1, the support of gasket 9 is not needed.The other structures of the embodiment are identical as embodiment two, and details are not described herein.
Embodiment three
As shown in fig. 6, the axis of tapered openings 7.3 and 7 shape of insect fixed plate are in an acute angle, insect head side is exposed to
7 surface of insect fixed plate makes insect head side be damaged after the impact force transmitted by crash panel 6.The embodiment its
Its structure is the same as example 1, and details are not described herein.
Example IV
As shown in fig. 7, the tapered openings 7.3 of fixed insect may include three parts: conical pipe 7.3.1, circular hole
7.3.3 and between conical pipe 7.3.1 and circular hole 7.3.3 it is used to fix the filler 7.3.2 of conical pipe 7.3.1.Wherein,
Conical pipe 7.3.1 can be used the suitably existing conical pipe truncation of size be made (such as common 1000ul in biological experiment,
200ul and 10ul loading pipette tips)；Circular hole 7.3.3 is the same circular hole of upper and lower diameter, and in favor of processing, diameter compares conical pipe
7.3.1 maximum gauge it is big；Silica gel, glass can be selected for conical pipe 7.3.1 to be fixed in circular hole 7.3.3 in filler 7.3.2
Glass glue etc..This scheme is easy to process, conical pipe 7.3.1 can size according to actual needs be adjusted and replace.The implementation
The other structures of example are the same as example 1, and details are not described herein.
Embodiment five
The experimental provision for establishing insect traumatic brain injury of the present embodiment, except whereabouts conduit 4 and upright bar 2 keep certain
Outside tilt angle, remaining is the same as embodiment one.
Particular embodiments described above has carried out further in detail the purpose of the present invention, technical scheme and beneficial effects
Describe in detail bright, it should be understood that the above is only a specific embodiment of the present invention, is not intended to restrict the invention, it is all
Within the spirit and principles in the present invention, any modification, equivalent substitution, improvement and etc. done should be included in protection of the invention
Within the scope of.
Claims (10)
1. a kind of controllable device for causing insect traumatic brain injury of dynamics characterized by comprising pedestal, upright bar, fixation
Part, conduit, weight, crash panel and insect fixed plate；
Wherein the upright bar is vertically arranged on the base；
The fixing piece is horizontally set in the upright bar, for fixing the conduit；
The crash panel is located at below the conduit；
The weight is placed in the conduit when in use and hits the crash panel when falling；
The insect fixed plate is located at the lower section of the crash panel, and the insect fixed plate has one or more insect fixation holes
With multiple spring mounting holes, support spring is provided in the spring mounting hole, the top of the support spring is higher than the elder brother
Worm fixed plate, the crash panel are arranged in the support spring.
2. the apparatus according to claim 1, wherein the conduit is made of clear material, preferably glass, transparent plastic
(such as PVC) etc., it is preferable that be labeled with scale on the inside or outside of the conduit, it is preferable that the conduit and the upright bar are flat
Row forms an inclination angle with the upright bar, it is preferable that the fixing piece is clip, it is preferable that the quantity of the fixing piece is
It is one or more.
3. the apparatus according to claim 1, wherein the weight is steel ball, it is preferable that described in the diameter ratio of the steel ball
The internal diameter of conduit is 0-2 millimeters small, it is highly preferred that being provided with drawstring on the weight.
4. the apparatus according to claim 1, wherein the body that the thickness of the insect fixed plate is greater than or equal to insect is long,
The shape of the insect fixed plate is disc, square or rectangular.
5. the apparatus according to claim 1, wherein the insect fixation hole is through-hole and opens under including upper opening
Mouthful, and the aperture of upper opening is less than the aperture of under shed, and the insect fixation hole is preferably bellmouth, it is highly preferred that described
1-1.5 times of the Zhou Changwei insect head circumference of upper opening, 1.5-2 times of the Zhou Changwei insect abdominal circumference maximum of under shed；It is excellent
Selection of land, the axis of the insect fixation hole are vertical with the insect fixed plate or in an acute angle with the insect fixed plate.
6. the apparatus according to claim 1, wherein the insect fixation hole includes the circle through the insect fixed plate
Hole, the conical pipe being arranged in the circular hole and the filler between the circular hole and the conical pipe, it is preferable that institute
Stating filler is silica gel or glass cement.
7. the apparatus according to claim 1, wherein the surrounding that the spring mounting hole is located at the insect fixed plate is uniform
Distribution, it is preferable that the quantity of the spring mounting hole is 3-8, it is preferable that the spring mounting hole is through-hole or groove, excellent
Selection of land, the spring mounting hole include bellmouth and cylindrical hole below, and the aperture of the upper opening of the bellmouth is greater than institute
The aperture of the under shed of bellmouth is stated, the under shed of the bellmouth is connected with the cylindrical hole, it is preferable that the bellmouth
The aperture of upper opening is 1.5-2.5 times of aperture of the under shed of the bellmouth, more preferably 2 times, under the bellmouth
The aperture of opening is identical as the aperture of the cylindrical hole, and the height of the bellmouth is 0.5-2 times of the height of the cylindrical hole,
The height of the more preferable bellmouth is identical as the height of the cylindrical hole.
8. the apparatus according to claim 1, wherein described device further includes gasket, and it is solid that the gasket is located at the insect
The lower section of fixed board, it is preferable that when the spring mounting hole is through-hole, the gasket is used to support the support spring.
9. the apparatus according to claim 1, wherein the shape of the shape and size of the crash panel and the insect fixed plate
Shape and size are identical or different, it is preferable that the material of the crash panel is selected from plastics (preferably PVC), metal, timber or glass
Glass, it is preferable that the shape of the crash panel is disc, square or rectangular, it is preferable that described device further includes being located at institute
State the insect collection vessel below insect fixed plate, it is preferable that the insect collection vessel is plate.
10. a kind of method for carrying out head injury experiment using any one of claim 1-9 described device, which is characterized in that
Include:
Insect is fixed in the insect fixation hole, and its head is made to be completely exposed to the surface of the insect fixed plate；
The weight is added in the conduit and is fallen on the crash panel under predetermined position, the impaction plates are made
The head of the insect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811561124.1A CN109479834B (en) | 2018-12-19 | 2018-12-19 | Device and method for causing traumatic brain injury of insects with controllable strength |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811561124.1A CN109479834B (en) | 2018-12-19 | 2018-12-19 | Device and method for causing traumatic brain injury of insects with controllable strength |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109479834A true CN109479834A (en) | 2019-03-19 |
| CN109479834B CN109479834B (en) | 2020-05-19 |
Family
ID=65710988
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811561124.1A Active CN109479834B (en) | 2018-12-19 | 2018-12-19 | Device and method for causing traumatic brain injury of insects with controllable strength |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109479834B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113876459A (en) * | 2021-09-30 | 2022-01-04 | 中国人民解放军陆军特色医学中心 | Animal injury-causing device capable of autonomously controlling impact force |
| CN114600790A (en) * | 2022-01-20 | 2022-06-10 | 中国人民解放军西部战区总医院 | Small animal blunt contusion striking system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101474441A (en) * | 2009-01-22 | 2009-07-08 | 湖州市农业科学研究院 | Combined injection machine for insect chrysalis |
| CN203354684U (en) * | 2013-06-17 | 2013-12-25 | 安徽正华生物仪器设备有限公司 | Rat brain injury model impactor |
-
2018
- 2018-12-19 CN CN201811561124.1A patent/CN109479834B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101474441A (en) * | 2009-01-22 | 2009-07-08 | 湖州市农业科学研究院 | Combined injection machine for insect chrysalis |
| CN203354684U (en) * | 2013-06-17 | 2013-12-25 | 安徽正华生物仪器设备有限公司 | Rat brain injury model impactor |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113876459A (en) * | 2021-09-30 | 2022-01-04 | 中国人民解放军陆军特色医学中心 | Animal injury-causing device capable of autonomously controlling impact force |
| CN113876459B (en) * | 2021-09-30 | 2023-11-07 | 中国人民解放军陆军特色医学中心 | Animal injury device capable of automatically controlling impact force |
| CN114600790A (en) * | 2022-01-20 | 2022-06-10 | 中国人民解放军西部战区总医院 | Small animal blunt contusion striking system |
| CN114600790B (en) * | 2022-01-20 | 2023-05-05 | 中国人民解放军西部战区总医院 | Blunt contusion striking system for small animals |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109479834B (en) | 2020-05-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109479834A (en) | A kind of device and method for causing insect traumatic brain injury that dynamics is controllable | |
| CN102841025B (en) | Drop hammer impact testing apparatus | |
| CN206341592U (en) | Oscillatory type garlic clove orients conveying device | |
| CN103263306B (en) | Device for modeling craniocerebral impact injuries of small animals | |
| CN106386451A (en) | Aeroponic cultivation device for observing growth development of plant | |
| CN201414851Y (en) | Animal closed brain injury falling body hitting device | |
| CN209356299U (en) | The bracket of impact position is adjusted in a kind of falling weight impact test | |
| CN207918853U (en) | Based on the cell culture apparatus for straining dynamic microenvironment in controllable analogue body | |
| CN109567975A (en) | A kind of device and method carrying out head injury experiment for fixing insect | |
| CN206314354U (en) | A kind of mist training device for observing growth and development of plants | |
| CN101947136A (en) | Miniature impact device for injuring experimental animals | |
| CN205826404U (en) | A kind of assay device for uniformly hitting real shaketalle test foundation model | |
| CN209060487U (en) | A kind of interior volleyball training frame | |
| CN206044772U (en) | A kind of Characters of traumatic brain injury analog | |
| CN2133837Y (en) | Simple experiment impactor | |
| CN107649083A (en) | A kind of use for laboratory reactor mounting platform | |
| RU172663U1 (en) | DEVICE FOR DETERMINING THE RANGE OF DISTRIBUTION OF SOIL PARTICLES | |
| CN206198085U (en) | A kind of big animal spinal cord moment impact damage model modeling beating device | |
| CN105943187B (en) | A kind of Characters of traumatic brain injury instrument | |
| CN208224495U (en) | A kind of corn plant height and fringe level measuring arrangement | |
| CN208511814U (en) | A kind of children training football device | |
| CN207756125U (en) | A kind of use for laboratory reaction kettle mounting platform | |
| CN107219135B (en) | Method for testing fly maggot recovery coefficient in vibration screening process | |
| CN216186695U (en) | Be used for cased original state soil sample of wax sealing to open box-packed and put | |
| RU102113U1 (en) | DEVICE FOR DYNAMIC LOADING OF THE TEST STRUCTURE WITH THE EXTENDED VIBILITY DAMPER |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |