CN109479605A - Arbuscular mycorrhizal fungi is applied in promoting dalbergia odorifera growth - Google Patents
Arbuscular mycorrhizal fungi is applied in promoting dalbergia odorifera growth Download PDFInfo
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- CN109479605A CN109479605A CN201811624614.1A CN201811624614A CN109479605A CN 109479605 A CN109479605 A CN 109479605A CN 201811624614 A CN201811624614 A CN 201811624614A CN 109479605 A CN109479605 A CN 109479605A
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- dalbergia odorifera
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Abstract
The invention discloses arbuscular mycorrhizal fungi to apply in promoting dalbergia odorifera growth.Heretofore described arbuscular mycorrhizal fungi is that Glomus versiforme, children cover that nearly bright sacculus is mould and at least one of a variety of AMF microbial inoculums of mixing.Find that arbuscular mycorrhizal fungi can be improved the indexs such as increment and biomass, photosynthesis, the chlorophyll fluorescence of seedling in the present invention, there are mutual promoting actions between root system of plant and edaphon, it can promote dalbergia odorifera seedlings root and mycorrhizal fungi Mutualism by mycorrhizal seedling raising technology, improve its growth.The present invention is dalbergia odorifera mycorrhizal seedling raising technology of establishing, and dalbergia odorifera strong sprout industrialized development provides technical support.
Description
Technical field
The present invention relates to soil microbiology, stress resistance of plant and plant nutrient fields, in particular to arbuscular mycorrhizal fungi
It is applied in promoting dalbergia odorifera growth.
Background technique
Dalbergia odorifera (Dalbergia odorifera T.Chen) also known as flower pears, rosewood, Huanghua Pear, fragrant redwood, flower
Palmitic acid wood, fragrant branch, are evergreen half deciduous tree of Papilionaceae, and it is precious to belong to the distinctive torrid zone of Chinese Second Class Key Protected Plant and China
Your tree species.It is that China 5 belongs to one of 34 kinds of 8 class precious redwood kinds.Wood quality is hard, and gloss is glossy, and fragrance is lasting, finishing
Slice decorative pattern is beautiful afterwards, indeformable after dry, do not crack, and is the valuable furniture of production and the superior material for carving exquisite craftwork.
Its also economic value with higher, medical value simultaneously, but since its heartwood growth time is longer, it is difficult to meet social need
It wants, in addition the excessive development and utilization of the mankind, long-term artificial felling causes its resource also very deficient.
(Zhou Xuegang, Zhuan Xueying, Wu Yongbin dalbergia odorifera seedling inoculation AM fungi effect study [J] forestry to Zhou Xuegang etc.
Practical technique, 2012 (8): 6-8.), (Mo Huizhi, Hong Wenjun, He Miaokun wait several AMF microbial inoculums of to dalbergia odorifera by Mo Huizhi etc.
Comparative studies [J] the Fujian Forestry science and technology of seedling mycorhiza effect, 2014 (3): 22-26.) and Liu Zijia etc. (Liu Zijia, Luo Jing,
Liu Fumei waits influence [J] the Chinese Urban Forestry of .AM fungi to dalbergia odorifera growth of seedling, 2015,13 (3): 35-38.) it grinds
Study carefully the research by inoculation AMF (arbuscular mycorrhizal fungi) to the growth of dalbergia odorifera and nutrient absorption, Mycorrhizal effect, inquires into seedling
Wood and Mycorrhizal Symbiosiss mechanism, the results showed that dalbergia odorifera seedling has stronger dependence to AMF.And inoculation AMF has nursery stock
Significant growth-promoting functions.Lu Junkun etc. (Lu Junkun, Xu great Ping, poplar were once encouraged, wait slowly raw rhizobium DG separation, identification and its with
Symbiosis [J] of dalbergia odorifera is applied and environmental organism journal, 2011,17 (3): 379-383.), (Xu Rui, Liu such as Xu Rui
Monarch is high, and Zhou Guoying waits dalbergia odorifera-santal rhizosphere soil efficient phosphate-solubilizing bacterium separation screening and identification [J] tropical crops
Journal, 2015,36 (2): 281-288.) and Xu Rui etc. (Xu Rui, Liu Junang, Luo Na wait dalbergia odorifera rhizosphere azotobacter to separate
Identification and bacterial activity research [J] soil notification, 2015 (5): 1121-1126.) it is directed to the nitrogen fixing capacity of dalbergia odorifera, carry out
Screening, the composite biological fertilizer specially development of dalbergia odorifera rhizobium and efficient phosphate-solubilizing bacterium, research promote its seedling growth and dross
Best Fertilizer Combinations.Therefore, dalbergia odorifera mycorrhizal research is always the focus of attention, but about dalbergia odorifera mycorrhizal seedlings
Screening, the report of Mycorrhizal seedling adaptability Journal of Sex Research are then relatively fewer.
Through literature search, the document that report is disclosed in relation to dalbergia odorifera and arbuscular mycorrhiza symbiosis is not yet retrieved.Using micro-
The growth of biological regulation plant also has more open application prospect to the resistance aspect of plant.To dalbergia odorifera resource
Protection, develop and utilize effective approach be provided, there are important economy, society and ecological benefits, therefore be also in recent years
Research hotspot.
Summary of the invention
The purpose of the present invention is to overcome the shortcomings of the existing technology and deficiency, provides arbuscular mycorrhizal fungi and is promoting dalbergia wood yellow
It is applied in wingceltis growth.
The purpose of the invention is achieved by the following technical solution: arbuscular mycorrhizal fungi is answered in promoting dalbergia odorifera growth
With, the arbuscular mycorrhizal fungi be Glomus versiforme, children cover that nearly bright sacculus is mould and a variety of AMF microbial inoculums of mixing at least one
Kind.
The arbuscular mycorrhizal fungi is preferably Glomus versiforme.
The Glomus versiforme is Glomus versiforme [Glomus versiforme (Karsten) Bench].
It is that children covers nearly bright mould [Glomus etunicatum (the Becker and of sacculus that it is mould, which to cover nearly bright sacculus, by the children
Gerdemann)]。
The inoculum concentration of the arbuscular mycorrhizal fungi is 50 ± 0.55g/ plants.
The arbuscular mycorrhizal fungi is applied in promoting dalbergia odorifera growth, for arbuscular mycorrhizal fungi is inoculated into dalbergia wood
On yellow wingceltis seedling；Or after mixing arbuscular mycorrhizal fungi with matrix, the matrix containing fungi is obtained, then dalbergia odorifera seedling is moved
It plants in the matrix containing fungi.
The matrix is yellow soil, river sand and bog moss soil；Preferably yellow soil, river sand and bog moss soil is by volume
The matrix being mixed to get for 2:1:1.
The dalbergia odorifera seedling is three months extremely annual dalbergia odorifera seedling；It is obtained preferably by following method
: dalbergia odorifera seed is peeled off into kind of a skin, is then placed in 60 DEG C of warm water and potassium permanganate is added and carry out disinfection, then is washed with water
It is impregnated after clean with clear water, sows, cultivates, obtain dalbergia odorifera seedling.
The disinfection is to be carried out disinfection using the potassium permanganate of mass fraction 0.5 ‰；Preferably use mass fraction
0.5 ‰ potassium permanganate sterilizes 30 minutes.
The time of the immersion is preferably 8 hours.
The sowing is to be seeded into seed in the sand bed after the disinfection of 5 ‰ potassium permanganate of mass fraction.
The time of the cultivation is preferably 3 months to 1 year；More preferably 3 months.
Application of the arbuscular mycorrhizal fungi in the bacteria agent that preparation promotes dalbergia odorifera growth, the arbuscular mycorrhiza are true
Bacterium is that Glomus versiforme, children cover that nearly bright sacculus is mould and at least one of a variety of AMF microbial inoculums of mixing.
The arbuscular mycorrhizal fungi is preferably Glomus versiforme.
A kind of dalbergia odorifera growth promoting bacteria agent, the growth promoting bacteria agent is Glomus versiforme, the nearly bright sacculus of children's set is mould and mixes
At least one of a variety of AMF microbial inoculums.
The growth promoting bacteria agent is preferably Glomus versiforme.
The present invention has the following advantages and effects with respect to the prior art:
1, the present invention provides a kind of with dalbergia odorifera symbiosis, and can be improved the increment of seedling and biomass, photosynthetic
The arbuscular mycorrhizal fungi screening technique of the indexs such as effect, chlorophyll fluorescence, to establish dalbergia odorifera mycorrhizal seedling raising technology, dalbergia wood
Yellow wingceltis strong sprout industrialized development provides technical support.
2, the present invention start to be inoculated with after tree seedling normal growth three months, wherein inoculation include Scotland sacculus it is mould,
Five kinds of different microbial inoculums such as table sacculus is mould, Moses's bucket pipe capsule is mould, the young closely bright mould, mix bacterium agent of sacculus of set.
3, the present invention applies artificial inoculation technique, by the mutual promoting action between root system of plant and edaphon,
The screening for probing into dalbergia odorifera adaptability microbial inoculum promotes dalbergia odorifera seedlings root and mycorrhizal fungi total by mycorrhizal seedling raising technology
It is raw mutually beneficial, improve its growth.
Detailed description of the invention
Fig. 1 is comparison result figure (the lowercase difference person in figure of dalbergia odorifera seedlings root situation under different AMF microbial inoculums
Indicate that the same processing difference reaches significantly under different microbial inoculums, P < 0.05)；Wherein, figure A is the comparison of root surface area；It is total for scheming B
The comparison of root long；Scheme the comparison that C is root volume；Scheme the comparison that D is average root diameter.
Fig. 2 is that (lowercase difference person indicates influence diagram of the different AMF microbial inoculums to dalbergia odorifera seedling Photosynthetic Index in figure
The same processing difference reaches significantly under different microbial inoculums, P < 0.05.)；Wherein, figure A is the comparison of Net Photosynthetic Rate (Pn value)；Figure
B is the comparison of stomatal conductance (Cond value), and figure C is intercellular CO2The comparison of concentration (Ci value)；Figure D is transpiration rate (Tr value)
Compare.
Fig. 3 is that the mycorhiza of 5 kinds of microbial inoculums after being inoculated with three months infects aspect graph；Wherein, figure A be infection court (amplification factor:
400×)；Scheming B is AMF vesicle (400 ×)；Figure C and D is AMF mycelia, vesicle, clump branch (200 ×).
Fig. 4 is that Gv group mycorhiza infects aspect graph；Wherein, figure A and B be infect mycelia (A:100 ×；B:400 ×).
Fig. 5 is that Gc group mycorhiza infects aspect graph；Wherein, figure A and B be infect mycelia (A:200 ×；B:400 ×).
Fig. 6 is that Fm group mycorhiza infects aspect graph；Wherein, figure A and B be infect mycelia (A:100 ×；B:200×).
Fig. 7 is that Ge group mycorhiza infects aspect graph；Wherein, figure A and B be infect mycelia (A:100 ×；B:100×).
Fig. 8 is that Mx group mycorhiza infects aspect graph；Wherein, figure A and B be Mx group mycorhiza infect situation (A:400 ×；B:200
×)。
Fig. 9 is not connect bacterium (ck) group mycorhiza to infect aspect graph；Wherein, figure A and B is that CK group mycorhiza infects situation respectively
(A:100×；B:400 ×).
Figure 10 is 5 kinds of microbial inoculum seedling growth situation maps of inoculation；Wherein, figure A is the ck group (left side 3 in figure compared with Gv group
Strain is ck group, and 3 plants of the right is Gv group)；Figure B is that (3 plants of the left side is Gc group to Gc group in figure, and 3 plants of the right is ck compared with ck group
Group)；Figure C is Gv group (3 plants of the left side is Gv group in figure, and 3 plants of the right is Mx group) compared with Mx group；Scheming D is Ge group and Mx group
Compare (3 plants of the left side is Ge group in figure, and 3 plants of the right is Mx group)；Scheming E is that (3 plants of the left side is Gc to Gc group in figure compared with Ge group
Group, 3 plants of the right are Ge group)；Figure F is Gc group (5 plants of the left side is Gc group in figure, and 3 plants of the right is Mx group) compared with Mx group；Scheme G
For ck group, Gc group compared with Ge group (3 plants of the left side is ck group in figure, and intermediate 3 plants are Gc group, and 3 plants of the right is Ge group)；Scheming H is
Gc group, Ge group compared with Gv group (3 plants of the left side is Gc group in figure, and intermediate 3 plants are Ge group, and 3 plants of the right is Gv group).
Figure 11 is 5 kinds of microbial inoculum nursery stock Nodule Growth situation maps of inoculation；Wherein, figure A is the nursery stock Nodule Growth situation of Mx group；
Scheme the nursery stock Nodule Growth situation that B is Fm group；Scheme the nursery stock Nodule Growth situation that C is Gc group；It is raw to scheme the nursery stock root nodule that D is Gv group
Long situation；Scheme the nursery stock Nodule Growth situation that E is Ge group；Scheme the nursery stock Nodule Growth situation that F is ck group.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
In the following examples, the experimental methods for specific conditions are not specified, usually according to normal condition.It is as used in the following examples each
Raw material and reagent can be obtained from commercially available in addition to particularly pointing out.
Embodiment 1
A kind of arbuscular mycorrhizal fungi screening technique promoting dalbergia odorifera growth includes the following steps: yellow to the raw dalbergia wood of 30d
Wingceltis sows the different arbuscular mycorrhizal fungi of five kinds of seedling inoculation, before being inoculated with the height of seedling of measurement dalbergia odorifera sowing seedling be 22.72 ±
1.78cm, ground diameter are 3.21 ± 0.26cm.
1. experimental material
It tests nursery stock: after the picking of dalbergia odorifera seed, peeling off kind of a skin, final concentration of quality point is added in 60 DEG C of warm water of seed
The potassium permanganate of number 5 ‰ sterilizes 30 minutes, and clear water cleans 2 times, and clear water impregnates 8 hours after cleaning.Sand bed mentions in warm canopy before sowing
Preceding use mass fraction is the disinfection of 5 ‰ potassium permanganate, and after planting to germination, pack is handled after one month.In January, 2017 starts
Sowing, packs after it grows 2 true leaves, starts to transplant seedlings April 9 in 2017 and apply bacterium.
2. testing microbial inoculum
Strain in the present invention is purchased from Tropical Forest Science Institute, China Forestry Science Research Institute, and Scotland sacculus is mould
[Glomus caledonium (Nicol.and Gerd.) Trappe&Gerd], abbreviation Gc；Glomus versiforme [Glomus
Versiforme (Karsten) Bench], abbreviation Gv；Moses's bucket mould [Funneliformis mosseae of pipe capsule
(T.H.Nicolson&Gerd.) C.Walker and A.Sch ü β ler)], abbreviation Fm；Children covers the nearly bright mould [Glomus of sacculus
Etunicatum (Becker and Gerdemann)], abbreviation Ge；Mix a variety of AMF microbial inoculums, abbreviation Mx.
3. potting media
Test matrix yellow soil (arboretum from Guangzhou university), commercially available gained river sand and the bog moss of purchase soil
(imported from Holland bog moss soil, Guangzhou is at large drug Co., Ltd) matrix, the ratio of 2:1:1 mixes by volume, sufficiently
It is pulverized after stirring, is packed after crossing 2mm sieve, carry out sterilizing 2h in high-pressure steam sterilizing pan, 126 DEG C of sterilising temp, after sterilizing
Mixed-matrix air-dry it is cooling after it is spare, soil physico-chemical property (table 1) is measured after sterilizing.
The 1 basic nutrient content of pot experiment soil of table
4. inoculation method
Bagged seedlings nutrition cup uses 18cm × 18cm specification, and every disk is inoculated with the above-mentioned strain of 50g.It is connect respectively in every basin nutrition cup
Kind Gv, Gc, Ge, Fm, Mx, not to be inoculated with bacterium as control (ck), totally 6 processing groups, every processing group set 15 repetitions for experiment, share
Test 90 basin of seedling.Specific step is as follows:
It is first packed into the mixed-matrix after the sterilizing of 1/3 height in two layers of nutrient bag, then equably spreads one layer respectively about
After the above-mentioned five kinds of bacterium of 50g, but sprinkle one layer sterilizing after mixed-matrix to basin it is high 3/4 at, thereon by seedling cultivation, be covered with
Mixed-matrix after one layer of sterilizing, the vermiculite (vermiculite is placed in alcohol in advance to be cleaned, dries) of last Surface mulch 50g, to prevent
Sealing point evaporation is too fast.Control group does not add any microbial inoculum, the mixed-matrix after the 50g sterilizing of equivalent is added in nutrient bag,
It is consistent to control variable.Every basin fills test matrix 500g, and vaccine wood will be connect after transplanting and is placed in a greenhouse conventional manage and protect.
5 measuring methods:
The measurement of 5.1 heights of seedling and ground diameter
Nursery stock height of seedling (cm) is measured using ruler, is used vernier caliper measurement seedling ground diameter (mm).
The measurement of 5.2 biomass
Using 105 DEG C of constant temperature drying methods.Plant complete stool is taken, plant each section is rinsed well with clear water, is dried, is packed into
Envelope is then placed in thermostatic drying chamber and first carries out 105 DEG C of water-removings processing 15min drying to constant weight at 80 DEG C again, writes down dry weight
Reading is amount of dry matter, and calculates root/shoot ratio (ratio of Underground biomass and Aboveground Biomass of Young).
The measurement of 5.3 root distinction coefficient values
To prevent from falling off, root system is washed away, chooses nursery stock 100 meshes of complete root system sample placement and is carefully rushed with clear water
Wash off the soil on root system surface.Root system sample image is acquired using EPSON Perfection V700Photo root scanner,
Software is analyzed with Win-Rhizo 2007c, measures full root distinction coefficient, including the total root long of root system, root system surface area, root system body
The root dry mass parameters such as long-pending and root average diameter.
The measurement of 5.4 Mycorrhizal Infection Incidences
Referring to PhilliPs and Hayman (1970), to infect the percentage of the total root segment of root segment Zhan as Mycorrhizal Infection Incidence,
Take part fresh root sample be fixed in FAA solution (- 50% ethanol solution of 37% formaldehyde-glacial acetic acid, volume ratio 9:0.5:
0.5) for detecting Mycorrhizal Infection Incidence.Specific method: first the fixer (FAA solution) of root is cleaned up, is immersed in 10%
(w/w) in KOH solution, 90 DEG C of heating water baths, until root sample becomes colorless and transparent.Transparent root segment is taken out, it is clear with flowing
Water gently cleans, and is then acidified 15min with the hydrochloric acid of 1% (w/w), and with acid fuchsin or trypan blue lactic acid glycerol (trypan blue:
0.12g, lactic acid 40ml；Glycerol 80ml；Distilled water: 80ml) stained over night, root is cut into the root segment of 2cm long, under the microscope
10 times of object lens observations, calculate Mycorrhizal Infection Incidence with crossing method.
The calculation formula of infection rate are as follows: infection rate=infect root segment length/root segment total length × 100%.
Mycorrhizal dependency (%)=processing group plant dry weight/control group dry weight * 100
Mycorrhizal dependency < 200% is weak dependence；200% < mycorrhizal dependency < 300% is medium dependence；Mycorhiza
Dependence > 300%, for strong dependency (Gong Mingqin, 2000).
5.5 photosynthesis characteristics index determinings
Select the functional leaf (specially from the terminal bud leaf that number the 5th~6 is taken turns down) of nursery stock portable photosynthetic with Li-6400
Function analysis system (U.S.) Yu Shangwu 9:00~11:30 is measured, and the red blue-light source of LI-6400-2B is used in continuous mode,
Light intensity is set as 1200 μm of olm-2·s-1, 30~35 DEG C of temperture of leaves setting, relative humidity 60% or so, Atmospheric CO2400 μ of concentration
mol·m-2·s-1, specifically include: the Net Photosynthetic Rate (Pn) of blade, intercellular CO2Concentration (Ci), stomatal conductance (Cond) and steaming
Rise the indexs such as rate (Tr).
6. experimental result
The present invention, with artificial inoculation technique, passes through root system of plant and edaphon using dalbergia odorifera as experimental material
Between mutual promoting action, probe into the adaptation Journal of Sex Research of dalbergia odorifera seedling and microbial inoculum, provided for dalbergia odorifera seedling-raising technique
The theoretical support with technology.Mycorrhizal Infection Incidence test experience is carried out in inoculation growth 90d (three months) afterwards, and each processing group mycorhiza infects
Rate comparison result is as shown in table 2.To inoculation 5 kinds of microbial inoculum dalbergia odorifera potting growth of seedling 180d after carry out photosynthetic, Root Characteristics
Deng experiment, as a result as shown in Tables 3 and 4.
Each processing group Mycorrhizal Infection Incidence compares after 2 dalbergia odorifera seedling inoculation of table is grown three months
| Strain | Gv | Gc | Ge | Mx | Fm | ck |
| Mycorrhizal Infection Incidence/% | 55.53±1.51a | 52.5±1.45a | 33.3±2.46ab | 32.56±3.11ab | 24.51±1.68b | 8.61±0.94c |
Note: significant difference (p < 0.05) between each column difference lowercase expression processing in table.
Influence of 3 different strain of table to dalbergia odorifera seedling net growth, biomass and root/shoot ratio is compared
| Strain | Height of seedling net growth/cm | Ground diameter net growth/mm | Amount of dry matter/g | Root/shoot ratio | Mycorhiza dependency degree |
| Gc | 30.78±1.51a | 4.58±0.54a | 8.86±1.19a | 0.33±0.05ab | 5.61±0.37a |
| Gv | 25.99±0.84a | 3.74±0.27a | 8.01±0.33a | 0.27±0.09ab | 5.07±0.48a |
| Ge | 20.37±1.34ab | 3.54±0.21a | 5.91±0.58b | 0.35±0.12ab | 3.74±0.25b |
| Mx | 18.25±2.34ab | 3.72±0.24a | 5.93±0.27b | 0.40±0.13a | 3.75±0.18b |
| Fm | 15.11±0.98b | 2.88±0.31ab | 4.10±0.25b | 0.31±0.05ab | 2.60±0.32c |
| ck | 11.60±1.11b | 2.85±0.29ab | 1.58±0.14c | 0.21±0.03b | 1.00±0.11d |
Note: significant difference (p < 0.05) between each column difference lowercase expression processing in table.
The membership function numerical value that table 4 respectively connects 9 growth indexes of bacterium group compares
Table 2 the results show that 5 kinds of microbial inoculums can form mycorhiza with dalbergia odorifera seedling infect, the height of infection rate be Gv >
Gc>Ge>Mx>Fm>ck.The infection rate for being inoculated with Gc, Gv group nursery stock is respectively 55.53 ± 1.51%, 52.5 ± 1.45%, significantly high
In other 3 kinds of microbial inoculums.Table 3 and table 4 the results show that the height of seedling and ground diameter net growth of inoculation AMF processing group, root system overall length,
Root volume, root surface area, average diameter are all larger than non-inoculation group；Meanwhile be inoculated with AMF also have to the variation of seedling Photosynthetic Response it is aobvious
Work property influences, and is inoculated with the Pn value (Net Photosynthetic Rate), Cond value (stomatal conductance), Ci value (intercellular CO of processing2Concentration), Tr value
(transpiration rate) is above non-inoculation group, and 5 kinds of AMF all have facilitation to the growth of dalbergia odorifera seedling, wherein inoculation Gc,
The effect of Gv group becomes apparent (Fig. 1 and 2).The mycorhiza of 5 kinds of microbial inoculums infects aspect graph as shown in figs. 3 to 9 after inoculation three months, nursery stock
Growing state is as shown in Figure 10, and Nodule Growth situation is as shown in figure 11.
Discussion and conclusion
The morphogenesis and growth of the dalbergia odorifera seedling plants of AMF (arbuscular mycorrhizal fungi) microbial inoculum are inoculated in the present invention
With obviously positive result, such as the significantly improving of increment, the increase of biomass, the growth of root system, osmotic field
The increase etc. of matter content improves photosynthetic efficiency to promote root system of plant to the absorption maximum of nutrient and moisture.
But difference AMF microbial inoculum is not of uniform size to the growth-promoting effect of dalbergia odorifera seedling, and the plant of inoculation Gv, Gc group is raw
Long pointer is above other microbial inoculums, it may be possible to be due to existing between plant and AMF because the adaptability of microbial inoculum and plant is different
Certain function compatibility, different plants, the compatibility of difference AMF microbial inoculum are different.In addition, though mycorhiza is not tight to host plant
The specificity of lattice, but still there is certain mutual selectivity in AMF and root system of plant.It is yellow with dalbergia wood that the purpose of the present invention is screenings
The best strain of wingceltis seedling finds that there are certain selections for microbial inoculum-plant-growth in the practical growth application study of AMF, so
Screening adapts to certain ecological condition and can form the efficient AMF microbial inoculum of optimal combination with host plant root system.
To in this present invention, compare the Mycorrhizal Infection Incidence of 5 kinds of microbial inoculums, finds different AMF to the regularity of infection of host plant
It is substantially close, but there is some difference.It is inoculated with the Mycorrhizal Infection Incidence of Gc, Gv group and dalbergia odorifera and the growth of nursery stock is promoted to make
Existed with, the accumulation of biomass, root system overall length, root surface area, average diameter, root volume and Net Photosynthetic Rate, stomatal conductance effect
It is better than other several microbial inoculums in varying degrees.It is comprehensive to be apparently inoculated with Gc group and dalbergia odorifera combined effect is best, it is secondly inoculation
Gv group.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
Claims (10)
1. arbuscular mycorrhizal fungi is applied in promoting dalbergia odorifera growth, it is characterised in that: the arbuscular mycorrhizal fungi is ground
Table sacculus is mould, the young closely bright sacculus of set is mould and at least one of a variety of AMF microbial inoculums of mixing.
2. arbuscular mycorrhizal fungi according to claim 1 is applied in promoting dalbergia odorifera growth, it is characterised in that:
The arbuscular mycorrhizal fungi is Glomus versiforme.
3. arbuscular mycorrhizal fungi according to claim 1 is applied in promoting dalbergia odorifera growth, it is characterised in that: described
Arbuscular mycorrhizal fungi inoculum concentration be 50 ± 0.55g/ plants.
4. arbuscular mycorrhizal fungi according to claim 1 is applied in promoting dalbergia odorifera growth, it is characterised in that: being will
Arbuscular mycorrhizal fungi is inoculated on dalbergia odorifera seedling；Or after mixing arbuscular mycorrhizal fungi with matrix, obtain containing fungi
Matrix, then by dalbergia odorifera seedling replanting into the matrix containing fungi.
5. arbuscular mycorrhizal fungi according to claim 4 is applied in promoting dalbergia odorifera growth, it is characterised in that:
The matrix is that yellow soil, river sand and bog moss soil are the matrix that 2:1:1 is mixed to get by volume.
6. arbuscular mycorrhizal fungi according to claim 4 is applied in promoting dalbergia odorifera growth, it is characterised in that:
The dalbergia odorifera seedling is three months extremely annual dalbergia odorifera seedling.
7. arbuscular mycorrhizal fungi according to claim 6 is applied in promoting dalbergia odorifera growth, which is characterized in that described
Dalbergia odorifera seedling obtain by the following method:
Dalbergia odorifera seed is peeled off into kind of a skin, is then placed in 60 DEG C of warm water and potassium permanganate is added and carry out disinfection, then is clear with water
It is impregnated after wash clean with clear water, sows, cultivates, obtain dalbergia odorifera seedling.
8. arbuscular mycorrhizal fungi according to claim 7 is applied in promoting dalbergia odorifera growth, it is characterised in that
The disinfection is to be sterilized 30 minutes using the potassium permanganate of mass fraction 0.5 ‰；
The time of the immersion is 8 hours；
The time of the cultivation is 3 months to 1 year.
9. application of the arbuscular mycorrhizal fungi in the bacteria agent that preparation promotes dalbergia odorifera growth, it is characterised in that: described
Arbuscular mycorrhizal fungi is that Glomus versiforme, children cover that nearly bright sacculus is mould and at least one of a variety of AMF microbial inoculums of mixing.
10. a kind of dalbergia odorifera growth promoting bacteria agent, it is characterised in that: the growth promoting bacteria agent is Glomus versiforme, the nearly bright ball of children's set
Capsule is mould and at least one of mixes a variety of AMF microbial inoculums.
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