CN109456893A - A kind of preparation method of light color high-density bacterial powder - Google Patents
A kind of preparation method of light color high-density bacterial powder Download PDFInfo
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- CN109456893A CN109456893A CN201811382320.2A CN201811382320A CN109456893A CN 109456893 A CN109456893 A CN 109456893A CN 201811382320 A CN201811382320 A CN 201811382320A CN 109456893 A CN109456893 A CN 109456893A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention belongs to field of biotechnology more particularly to a kind of preparation methods of light high-density bacterial powder.It is to ferment the fresh bacteria residue liquid after being separated by solid-liquid separation as raw material using nucleosides, nucleotide or amino acids industry, by low-temperature evaporation concentration, biological enzymolysis, high-temperature inactivation and is spray-dried obtained product.Spray drying is using spray dryer, and material is first heated to 90-100 DEG C by heater, is pumped into drying tower feed inlet by feeding, injection pressure is 4.5-5.0MPa, and drying tower inlet air temperature is 190-210 DEG C, and exhaust temperature is 90-110 DEG C.Mycoprotein powder product density prepared by the present invention reaches 0.50g/cm3More than, in micro mist it is granular, have apparent solubility it is big, water imbibition is low, good fluidity, it is prevented from caking the advantages that.
Description
Technical field
The invention belongs to field of biotechnology, disclose a kind of preparation method of light high-density bacterial powder.
Background technique
Bacteria residue is to be filtered in fermentation industry to fermentation liquid, collects filtrate, dumps bacteria residue.In bacteria residue in addition to water, main
If usual this bacteria residue is as offal treatment to produce based on the thallus of bacterium.Nucleosides, nucleotide or amino acids industry fermentation
The extracted discarded thallus being refined into after product of liquid is contained a kind of single cell protein manufactured after separation, drying, milling
Protein abundant and other nutriments.The protein content of the mycoprotein is up to 50% or more, than plant animal class egg
White up to 20%.Amino acid classes are more complete, wherein rich in threonine, valine, methionine, lysine, leucine, different
Several essential amino acids of leucine, phenylalanine.And such mycoprotein Pure natural nontoxic, meet food safety quality and wants
It asks.If such bacteria residue, which directly dumps, had not only polluted environment but also waste of resource.
Bacteria residue is because of protein content height, and full of nutrition, easily growth miscellaneous bacteria microorganism carries out secondary fermentation, keeps bacteria residue liquid corrupt
It is rotten to generate foul smell, environment is polluted, is stored naturally so being not easy the long period.Again because moisture content is big, up to 80% or more, very
Hardly possible realizes that industrialization is continuous drying.Traditional drying such as air blast plate is dry, and product is blocking, and easy coking, color is pitch black, close after crushing
Spend low, fugitive dust.Drying time is long, and treating capacity is small, and energy consumption is high, at high cost, is not suitable for production.
Summary of the invention
In view of the above-mentioned problems, the present invention provides a kind of thallus eggs that thallus utility value is high, environmentally protective, full of nutrition
The production method of white powder.
In order to solve the above technical problems, present invention provide the technical scheme that a kind of light color high-density bacterial powder
Preparation method, the fresh bacteria residue liquid after being separated by solid-liquid separation using the fermentation of nucleosides, nucleotide or amino acids industry is raw material, through too low
Product is made in warm evaporation and concentration, biological enzymolysis, high-temperature inactivation and spray drying.
Further, the spray drying is using spraying in the preparation method of above-mentioned light high-density bacterial powder
Drier, material are first heated to 90-100 DEG C by heater, are pumped into drying tower feed inlet by feeding, injection pressure is
4.5-5.0MPa, drying tower inlet air temperature are 190-210 DEG C, and exhaust temperature is 90-110 DEG C.
The low-temperature evaporation concentration, which refers to, carries out low temperature to the fresh bacteria residue liquid after filtration washing using three-level inspissator
It is concentrated by evaporation, 85-95 DEG C of level-one temperature, 70-80 DEG C of second level temperature, 60-65 DEG C of three-level temperature, concentration time 6-8 hours；Control
Dry substance processed is 15-20%, puts furnace temperature lower than 65 DEG C, 13Be, Be are a kind of methods for indicating solution concentration, Baume ratio
Restatement immerses in surveyed solution, and obtained degree is just Baume degrees.
It is 15-20% that the biological enzymolysis, which is with 45-55 DEG C of hot water adjustment bacteria residue concentrate dry substance, stirs evenly, uses
Acid or adjusting PH with base value are 6.0~7.0；Addition bacteria residue liquid dry substance ratio is 0.2%~0.6% compound protease, temperature regulating 55
~62 DEG C, carry out 6-12 hours constant temperature enzyme digestion reactions.
The high-temperature inactivation is carried out quickly being heated to 75-85 DEG C for enzymolysis liquid, keeps the temperature 30min in enzymatic hydrolysis terminal,
High-temperature inactivation is carried out to biological enzyme, terminates its activity.
Further: in the preparation method of above-mentioned light high-density bacterial powder, the spray dryer includes gas
Body heater and the drying tower being attached thereto, are also connected with sprayer and heat distributor, gas in turn on the interior top of drying tower
Heater is connected by hot air pipe with the heat distributor at the top of drying tower.The spray dryer further includes having tubular type heating
Device and feeding engine, the tube heater are connected by feed pipe with feeding engine, and feeding engine passes through feed pipe and drying tower
Feed inlet be connected, tube heater formed the first heating zone.
Compared with prior art, the preparation method of high-density bacterial powder of the present invention, with nucleosides, nucleotide or amino acid
Industrial fermentation be separated by solid-liquid separation after fresh bacteria residue liquid be raw material, by low-temperature evaporation concentration, biological enzymolysis, high-temperature inactivation and
It is spray-dried and product is made.Present invention spray drying is using spray dryer, and material is first heated to 90- by heater
100 DEG C, drying tower feed inlet is pumped by feeding, injection pressure is 4.5-5.0MPa, and drying tower inlet air temperature is 190-210
DEG C, exhaust temperature is 90-110 DEG C.Sprayer of the invention is mounted on drying tower internal upper part, so that the thallus after the present invention is dry
Albumen pink colour is shallow and density is big.To improve mycoprotein powder quality and can production drying means.On raw material sources
Cheap, protein content is high；Less energy consumption in production process, not will cause secondary pollution；Bacteria residue is uniformly dispersed, than meat, beans
Dregs of rice reaction is more abundant, has richer nutritive value as feed addictive.Mycoprotein powder product prepared by the present invention
Density reaches 0.50g/cm3 or more, granular in micro mist, has apparent solubility big, water imbibition is low, good fluidity, prevented from caking
The advantages that.
Detailed description of the invention
Fig. 1 is spray dryer structural schematic diagram used in 1-5 of the embodiment of the present invention；
Wherein: 1 gas heater, 2 drying towers, 3 sprayers, 4 heat distributors, 5 hot air pipes, 6 tube heaters, 7 feedings
Pump, 8 feed pipes.
Specific embodiment
The contents of the present invention are described in further detail below with reference to embodiment, the content mentioned in embodiment is not to this
The restriction of invention, in preparation process the selection of each raw material can adaptation to local conditions and substantial effect is had no to result.
Embodiment 1
A kind of preparation method of light color high-density bacterial powder, is fermented with nucleosides, nucleotide or amino acids industry and is carried out
Fresh bacteria residue liquid after separation of solid and liquid is raw material, is made by low-temperature evaporation concentration, biological enzymolysis, high-temperature inactivation and spray drying
Product.The spray drying is using spray dryer, and material is first heated to 90 DEG C by heater, is pumped by feeding
Drying tower feed inlet, injection pressure are 4.9MPa, and drying tower inlet air temperature is 200 DEG C, and exhaust temperature is 100 DEG C.Described is low
Temperature, which is concentrated by evaporation, to be referred to using three-level inspissator to the fresh bacteria residue liquid progress low-temperature evaporation concentration after filtration washing, level-one temperature
88 DEG C, 78 DEG C of second level temperature, 62 DEG C of three-level temperature, concentration time 7 hours；Controlling dry substance is 20%, puts furnace temperature lower than 65
DEG C, 13Be.It is 17% that the biological enzymolysis, which is with 46 DEG C of hot water adjustment bacteria residue concentrate dry substances, is stirred evenly, with acid or alkali
Adjusting pH value is 6.5；Addition bacteria residue liquid dry substance ratio is 0.4% compound protease, and temperature regulating is 60 DEG C, carries out 10 hours constant temperature enzymes
Solution reaction.The high-temperature inactivation is carried out quickly being heated to 82 DEG C for enzymolysis liquid, 30min is kept the temperature, to life in enzymatic hydrolysis terminal
Object enzyme carries out high-temperature inactivation, terminates its activity.Mycoprotein powder ρ heap=0.56g/cm produced3。
Embodiment 2
A kind of preparation method of light color high-density bacterial powder, is fermented with nucleosides, nucleotide or amino acids industry and is carried out
Fresh bacteria residue liquid after separation of solid and liquid is raw material, is made by low-temperature evaporation concentration, biological enzymolysis, high-temperature inactivation and spray drying
Product.The spray drying is using spray dryer, and material is first heated to 92 DEG C by heater, is pumped by feeding
Drying tower feed inlet, injection pressure are 4.5MPa, and drying tower inlet air temperature is 210 DEG C, and exhaust temperature is 110 DEG C.Described is low
Temperature, which is concentrated by evaporation, to be referred to using three-level inspissator to the fresh bacteria residue liquid progress low-temperature evaporation concentration after filtration washing, level-one temperature
93 DEG C, 78 DEG C of second level temperature, 62 DEG C of three-level temperature, concentration time 7 hours；Controlling dry substance is 17%, puts furnace temperature lower than 65
DEG C, 13Be.It is 15% that the biological enzymolysis, which is with 48 DEG C of hot water adjustment bacteria residue concentrate dry substances, is stirred evenly, with acid or alkali
Adjusting pH value is 6.8；Addition bacteria residue liquid dry substance ratio is 0.5% compound protease, and temperature regulating is 58 DEG C, carries out 9 hours constant temperature enzymes
Solution reaction.The high-temperature inactivation is to carry out enzymolysis liquid to be quickly heated to 76 DEG C in enzymatic hydrolysis terminal, keeps the temperature 30min, right
Biological enzyme carries out high-temperature inactivation, terminates its activity.Mycoprotein powder ρ heap=0.58g/cm produced3。
Embodiment 3
A kind of preparation method of light color high-density bacterial powder, is fermented with nucleosides, nucleotide or amino acids industry and is carried out
Fresh bacteria residue liquid after separation of solid and liquid is raw material, is made by low-temperature evaporation concentration, biological enzymolysis, high-temperature inactivation and spray drying
Product.The spray drying is using spray dryer, and material is first heated to 95 DEG C by heater, is pumped by feeding
Drying tower feed inlet, injection pressure are 4.7MPa, and drying tower inlet air temperature is 200 DEG C, and exhaust temperature is 100 DEG C.Described is low
Temperature, which is concentrated by evaporation, to be referred to using three-level inspissator to the fresh bacteria residue liquid progress low-temperature evaporation concentration after filtration washing, level-one temperature
90 DEG C, 75 DEG C of second level temperature, 64 DEG C of three-level temperature, concentration time 7.2 hours；Controlling dry substance is 18%, puts furnace temperature and is lower than
65 DEG C, 13Be.It is 16% that the biological enzymolysis, which is with 46 DEG C of hot water adjustment bacteria residue concentrate dry substances, is stirred evenly, with acid or
Adjusting PH with base value is 6.9；Addition bacteria residue liquid dry substance ratio is 0.6% compound protease, and temperature regulating is 60 DEG C, carries out 10 hours constant temperature
Enzyme digestion reaction.The high-temperature inactivation is to carry out enzymolysis liquid to be quickly heated to 79 DEG C in enzymatic hydrolysis terminal, keeps the temperature 30min, right
Biological enzyme carries out high-temperature inactivation, terminates its activity.Mycoprotein powder ρ heap=0.55g/cm produced3。
Embodiment 4
A kind of preparation method of light color high-density bacterial powder, is fermented with nucleosides, nucleotide or amino acids industry and is carried out
Fresh bacteria residue liquid after separation of solid and liquid is raw material, is made by low-temperature evaporation concentration, biological enzymolysis, high-temperature inactivation and spray drying
Product.The spray drying is using spray dryer, and material is first heated to 97 DEG C by heater, is pumped by feeding
Drying tower feed inlet, injection pressure are 4.9MPa, and drying tower inlet air temperature is 205 DEG C, and exhaust temperature is 105 DEG C.Described is low
Temperature, which is concentrated by evaporation, to be referred to using three-level inspissator to the fresh bacteria residue liquid progress low-temperature evaporation concentration after filtration washing, level-one temperature
92 DEG C, 75 DEG C of second level temperature, 62 DEG C of three-level temperature, concentration time 7 hours；Controlling dry substance is 19%, puts furnace temperature lower than 65
DEG C, 13Be.It is 18% that the biological enzymolysis, which is with 45-55 DEG C of hot water adjustment bacteria residue concentrate dry substance, is stirred evenly, with acid or
Adjusting PH with base value is 7.0；Addition bacteria residue liquid dry substance ratio is 0.55% compound protease, and temperature regulating is 61 DEG C, carries out 11 hours perseverances
Warm enzyme digestion reaction.The high-temperature inactivation is to carry out enzymolysis liquid to be quickly heated to 75-85 DEG C, heat preservation in enzymatic hydrolysis terminal
30min carries out high-temperature inactivation to biological enzyme, terminates its activity, mycoprotein powder ρ heap=0.57g/cm produced3。
Embodiment 5
A kind of preparation method of light color high-density bacterial powder, is fermented with nucleosides, nucleotide or amino acids industry and is carried out
Fresh bacteria residue liquid after separation of solid and liquid is raw material, is made by low-temperature evaporation concentration, biological enzymolysis, high-temperature inactivation and spray drying
Product.The spray drying is using spray dryer, and material is first heated to 100 DEG C by heater, is pumped by feeding
Enter drying tower feed inlet, injection pressure is 5.0MPa, and drying tower inlet air temperature is 190 DEG C, and exhaust temperature is 90 DEG C.Described is low
Temperature, which is concentrated by evaporation, to be referred to using three-level inspissator to the fresh bacteria residue liquid progress low-temperature evaporation concentration after filtration washing, level-one temperature
87 DEG C, 75 DEG C of second level temperature, 62 DEG C of three-level temperature, concentration time 7 hours；Controlling dry substance is 18%, puts furnace temperature lower than 65
DEG C, 13Be.It is 15% that the biological enzymolysis, which is with 48 DEG C of hot water adjustment bacteria residue concentrate dry substances, is stirred evenly, with acid or alkali
Adjusting pH value is 6.5；Addition bacteria residue liquid dry substance ratio is 0.4% compound protease, and temperature regulating is 57 DEG C, carries out 8 hours constant temperature enzymes
Solution reaction.The high-temperature inactivation is to carry out enzymolysis liquid to be quickly heated to 77 DEG C in enzymatic hydrolysis terminal, keeps the temperature 30min, right
Biological enzyme carries out high-temperature inactivation, terminates its activity, mycoprotein powder ρ heap=0.56g/cm produced3。
Spray dryer used in 1-5 in the above-described embodiments as shown in Figure 1:, it includes gas heater 1 and connects therewith
The drying tower 2 connect is also connected with sprayer 3 and heat distributor 4 in turn on the interior top of drying tower, and gas heater passes through hot wind
Pipe 5 is connected with the heat distributor at the top of drying tower.Spray dryer further includes having tube heater 6 and feeding engine 7, described
Tube heater is connected by feed pipe 8 with feeding engine, and feeding engine is connected by feed pipe with the feed inlet of drying tower.
Sprayer of the invention is mounted on drying tower internal upper part, so that mycoprotein powder product density prepared by the present invention reaches
The advantages that 0.50g/cm3 or more, granular in micro mist, big with apparent solubility, water imbibition is low, good fluidity, prevented from caking.And
Spray dryer is simple to operation.
Claims (7)
1. a kind of preparation method of light color high-density bacterial powder, is consolidated with the fermentation of nucleosides, nucleotide or amino acids industry
Fresh bacteria residue liquid after liquid separation is raw material, is made and produces by low-temperature evaporation concentration, biological enzymolysis, high-temperature inactivation and spray drying
Product.
2. the preparation method of light color high-density bacterial powder according to claim 1, it is characterised in that: described spraying dry
Dry is using spray dryer, and material is first heated to 90-100 DEG C by heater, is pumped into drying tower charging by feeding
Mouthful, injection pressure is 4.5-5.0MPa, and drying tower inlet air temperature is 190-210 DEG C, and exhaust temperature is 90-110 DEG C.
3. the preparation method of light color high-density bacterial powder according to claim 2, it is characterised in that: the low temperature
It is concentrated by evaporation and refers to using three-level inspissator to the fresh bacteria residue liquid progress low-temperature evaporation concentration after filtration washing, level-one temperature
85-95 DEG C, 70-80 DEG C of second level temperature, 60-65 DEG C of three-level temperature, concentration time 6-8 hours；Control dry substance is 15-20%,
It puts furnace temperature and is lower than 65 DEG C, 13Be.
4. the preparation method of light color high-density bacterial powder according to claim 3, it is characterised in that: the biological enzyme
It is 15-20% that solution, which is with 45-55 DEG C of hot water adjustment bacteria residue concentrate dry substance, is stirred evenly, with acid or adjusting PH with base value be 6.0~
7.0；Addition bacteria residue liquid dry substance ratio is 0.2%~0.6% compound protease, and temperature regulating is 55~62 DEG C, is carried out 6-12 hours
Constant temperature enzyme digestion reaction.
5. the preparation method of light color high-density bacterial powder according to claim 4, it is characterised in that: the high temperature goes out
Work is carried out quickly being heated to 75-85 DEG C for enzymolysis liquid, keeps the temperature 30min in enzymatic hydrolysis terminal, is carried out high temperature to biological enzyme and is gone out
It is living, terminate its activity.
6. the preparation method of light color high-density bacterial powder according to claim 2, it is characterised in that: described spraying dry
Dry device includes gas heater (1) and the drying tower (2) being attached thereto, and is also connected with sprayer in turn on the interior top of drying tower
(3) it is connected by hot air pipe (5) with the heat distributor at the top of drying tower with heat distributor (4), gas heater.
7. the preparation method of light color high-density bacterial powder according to claim 6, it is characterised in that: described is sprayed
Drier further includes having tube heater (6) and feeding engine (7), and the tube heater passes through feed pipe (8) and feeding engine
It is connected, feeding engine is connected by feed pipe with the feed inlet of drying tower.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110713394A (en) * | 2019-11-11 | 2020-01-21 | 广东肇庆星湖生物科技股份有限公司 | Preparation method of amino acid foliar fertilizer |
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