CN1051445C

CN1051445C - Production of fodder protein by fermenting filtered mud

Info

Publication number: CN1051445C
Application number: CN93121183A
Authority: CN
Inventors: 傅锦通
Original assignee: Individual
Current assignee: Individual
Priority date: 1993-12-28
Filing date: 1993-12-28
Publication date: 2000-04-19
Anticipated expiration: 2013-12-28
Also published as: CN1104440A

Abstract

The present invention discloses a technical method for producing feed protein by using mud fermentation. Aspergillus niger and candida tropicalis which can grow well in mud are chosen. A process that mixed fermentation on a solid medium is carried out after the submerged fermentation of monoxenie liquid is adopted. Solid fermentation is carried out in a solid ventilating fermentation machine. Drying means instant dehydration by using hot air in a flow state drying machine. The whole drying process is controlled within 2 seconds, and temperature is not more than 50 DEG C all the time. The technology is suitable for production with an industrialized continuous fermentation method. The technology has the advantages of simpleness, reliability, little investment for equipment and high productive efficiency and not only solves the problem of environmental pollution but also develops new feed protein sources.

Description

Utilize filter mud fermentation to produce the technology of forage protein

The present invention relates to a kind of process of fermenting and producing forage protein, it is not only applicable to filter mud as raw materials for production, and also can be suitable for bean dregs, vinasse, peanut cake is that raw material is produced forage protein.

As everyone knows, a large amount of mud waste things can appear when adopting cane sugar manufacture, 10 tons of sucrose of every production just have 1 ton dry filter mud, and this dry filter mud comprises sucrose 16-22%, crude fat 8.5%, thick dimension fine 8.2%, crude protein 13.3%, calcium 5.6%, phosphorus 0.93%, sulphur 2.1% also has a spot of magnesium, zinc, potassium, sodium, manganese, cobalt, copper, iron etc.At present domestic all is filter mud to be piled up allow the base manure as fruit tree uses after its spontaneous fermentation, environment is caused to a certain degree pollution, I once proposed " utilizing filter mud to produce the method for edible mushroom " in Chinese patent CN87102933 number for this reason, adopt this method that filter mud is turned waste into wealth, minimizing is to the pollution of environment, the edible mushroom that produces can be edible, but its application has certain limitation, popularize certain degree of difficulty in an all-round way.

Task of the present invention is that a kind of technology of utilizing filter mud fermentation to produce forage protein will be provided, it is applicable to the production of industrialization continuous fermentation method, its technology is simple, reliable, equipment investment is few, the productivity effect height has not only been administered the pollution of environment, and has developed new forage protein source, for surviving the winter of ruminant domestic animal opened up a new grain source, for " transferring Southern grain to the North " engineering that realizes China's feed is opened up an approach.

Task of the present invention is finished in such a way, this production technology adopted can be in filter mud well-grown aspergillus niger and candida tropicalis, using liquid, solid combined ferment, promptly use mixed culture fermentation on solid medium after single bacteria liquid submerged fermentation, thereby obtain to contain crude protein and reach 30%, and contain the forage protein of abundant vitamin, digestive ferment and an amount of inorganic salts, solid fermentation is to carry out in the ventilating fermentation machine, in order to help fermentation, it is partially mixed that nitrogenous source adopts, and the form that adds of stream is carried out in batches.Consider from the chemical composition of filter mud that simultaneously it is a height sugar amount matrix, and contain and definite by orthogonal test aspect nitrogenous source, nitrogen amount proportioning, can supply the carbon of fermentation for the various inorganic salts of fermentation that nitrogen is than rational solid fermentation culture medium thereby set up.Following mask body is introduced its production technology:

One, the making of culture medium

1, yeast slant medium: the raw material (not hopping) with fermentation beer, be diluted to 12-15 Berlin, add 1000 milliliters in water, add 15 in agar, dissolve the back packing, sterilized 20 minutes for 15 pounds.

2, mould slant medium: get filter mud 200 gram, wheat skin 20 grams, soya-bean cake powder 20 grams add 1000 milliliters in water, soak 1 hour, boil 20 minutes, cross leaching juice and are diluted to 1000 milliliters and add agar 15 grams, dissolve the back packing, and pH value is taken from right value, sterilizes 1 hour for 15 pounds.

3, yeast fluid nutrient medium: get bright filter mud 200 grams, add 1000 milliliters in water, agitation and filtration is got 5 ° of BX brewer's worts of juice and equivalent and is mixed, and pH value is taken from right value, sterilizes 20 minutes for 15 pounds.

4, mould fluid nutrient medium: get bright filter mud 200 grams, wheat skin 20 grams, soya-bean cake powder 20 grams add 1000 milliliters in water, soak 1 hour, boil 20 minutes, cross leaching juice and are diluted to 1000 milliliters, and pH value is taken from right value, sterilizes 20 minutes for 15 pounds.

5, solid fermentation culture medium: get bright filter mud (moisture 65%) and account for 90%, soya-bean cake powder 4%, wheat skin 4%, urea 1%, ammonium sulfate 1%, PH are taken from right value, and moisture content transfers to 60%, 80 ℃ of sterilization 20 minutes.

Two, the female making of planting in inclined-plane: place 30 ℃ of incubators to cultivate 48 hours in yeast, mould places 30 ℃ of incubators to cultivate 6 days.

Three, liquid shaking bottle operation: it is that 220 rev/mins shaking table was cultivated 24 hours under 30 ℃ of room temperatures that yeast is placed rotating speed; Mould is placed 220 rev/mins shaking table, cultivated 36 hours for 30 ℃.

Four, seed tank culture: yeast is placed 220 rev/mins of shaking tables, and room temperature is 30 ℃, and throughput is 1: 0.8, cultivates 36 hours; Mould is in rotating speed is 330 rev/mins shaking table, and 30 ℃, throughput is 1: 0.8, cultivates 36 hours.

Five, solid fermentation: solid sterilization is cooled to 35 ℃ then and enters in the aerobic fementation machine and be mixed in 30 ℃ in fermentation 48 hours with yeast, mould.

Six, oven dry, packing: product carries out in the fluidised form dryer, utilizes the hot blast instant dehydration, controls whole drying course and is no more than 2 seconds, and temperature is no more than 50 ℃ from start to finish.

Adopt above-mentioned explained hereafter forage protein, destructiveness to effective ingredients such as protein, vitamin, digestive ferments is few, crude protein can reach 31%, amylase activity is greater than 3000, black mold has effectively comprehensively enzyme system, and product also has the cellulase system of a great deal of, zytase, protease etc. can be used as a kind of desirable poultry and livestock feed albumen.

Claims

1. process of utilizing filter mud fermentation to produce forage protein, it is characterized in that: select aspergillus niger and candida tropicalis for use, the solid medium per kilogram contains bright filter mud (moisture 65%) and accounts for 90%; Soya-bean cake powder 4%, wheat skin 4%, urea 1%, ammonium sulfate 1%, PH takes from right value, moisture content transfers to 60%, is cooled to 35 ℃ through 80 ℃ of sterilizations after 20 minutes, enters the aerobic fementation machine and mixes with yeast, mould, in 30 ℃ of fermentations 48 hours, oven dry utilizes the hot blast instant dehydration to carry out in the fluidised form dryer, controls whole drying course and is no more than 2 seconds, and temperature is no more than 50 ℃ all the time.