CN109453439B - Mucous membrane protective agent for artificial insemination of livestock and preparation method thereof - Google Patents
Mucous membrane protective agent for artificial insemination of livestock and preparation method thereof Download PDFInfo
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- CN109453439B CN109453439B CN201910059088.7A CN201910059088A CN109453439B CN 109453439 B CN109453439 B CN 109453439B CN 201910059088 A CN201910059088 A CN 201910059088A CN 109453439 B CN109453439 B CN 109453439B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/005—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/14—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L31/16—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/452—Lubricants
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Abstract
The invention relates to the technical field of artificial insemination of livestock, in particular to a mucosa protective agent for artificial insemination of livestock and a preparation method thereof, which comprises 90-150 parts of matrix, 1-15 parts of bacteriostatic agent, 10-30 parts of emulsifying thickener and 0.1-0.5 part of antioxidant; the invention is liquid, have very good lubricity, can pour into the female livestock vagina and cervix uteri position conveniently, and adhere to vagina and cervix uteri mucosa, play a role in lubricating, bacteriostasis and protecting mucosa when carrying on the artificial insemination operation, the raw materials in the invention are all pharmaceutical grade supplementary products, there is no oxic side effect, will not cause the semen property change of artificial insemination, will not influence the vigor of the sperm, and there is certain antibacterial capacity, avoid the female livestock vagina mucosa, cervix uteri from being damaged and infected.
Description
Technical Field
The invention relates to the technical field of artificial insemination of livestock, in particular to a mucosa protective agent for artificial insemination of livestock and a preparation method thereof.
Background
With the rapid development of animal husbandry, female livestock plays a decisive role in the breeding benefit as a key link in the breeding production process, and modern intensive farms mostly adopt an artificial fertilization method to breed livestock in order to improve the production benefit. Artificial insemination requires insertion into the vagina of a female animal by means of a vas deferens, and then injection of previously prepared semen into the female animal. The vas deferens is divided into a plurality of times of use and a disposable one, on one hand, the sponge head of the vas deferens has certain friction, on the other hand, the disinfection and sterility guarantee measures of the vas deferens are imperfect in the process of artificial insemination, and the sponge head of the disposable vas deferens is often coated with a lubricant to protect the mucosa of the maternal and continuous vagina from being damaged by friction.
CN201610947042.5 discloses a lubricant and a preparation method and application thereof, 0.1-1.0% of a thickening agent, 0.1-1.0% of a lubricant, 3.0-10.0% of a humectant, 0.1-2.0% of a pH value regulator, 0.3-2.0% of a preservative, and the balance of purified water; the thickening agent is at least one of carbomer resin, guar gum, xanthan gum, polyvinylpyrrolidone and polyacrylamide; the lubricant is at least one of tremella polysaccharide, blue silk skin beautifying agent, sour bean seed polysaccharide and carrageen crispus; the humectant is at least one of glycerol, butanediol, water-soluble luba gum oil, betaine, panthenol and propylene glycol; the preservative is at least one of senviva SC10, Spectrastat PHL, grapefruit extract and PA 40.
However, in the prior art, the lubricant for artificial insemination is in a gel state, and cannot be uniformly distributed on the surface of vaginal mucosa in the process of inserting the vas deferens into the vagina, so that the vaginal mucosa or uterine mucosa is often damaged, the vaginal mucosa or uterine mucosa of the female livestock is easily subjected to mechanical damage or bacterial infection, and the lubricant has no bacteriostatic action, so that the vagina or uterus infection of the female livestock is easily infected and inflamed. In addition, the existing lubricant can easily inactivate the sperms of the livestock and can not meet the requirement of artificial fertilization.
Disclosure of Invention
The invention aims to provide a mucosa protective agent for artificial insemination of livestock, which adopts a compound preparation and carefully selects raw material medicaments and has excellent lubricating, bacteriostasis and mucosa protection performances.
The invention also aims to provide a preparation method of the mucosa protective agent for the artificial insemination of the livestock, which has scientific and reasonable process and is simple and easy to implement.
In order to achieve the purpose, the invention adopts the following technical scheme:
a mucosa protective agent for livestock artificial insemination is characterized by comprising the following raw materials in parts by mass: 90-150 parts of matrix, 1-15 parts of bacteriostatic agent, 1-30 parts of emulsifying thickener and 0.1-0.5 part of antioxidant.
The matrix is preferably medicinal white oil, medicinal liquid paraffin and medicinal ethyl oleate; the medicinal white oil, the medicinal liquid paraffin and the medicinal ethyl oleate are equal in parts.
The bacteriostatic agent is preferably origanum oil, herba Schizonepetae volatile oil, Curcumae rhizoma volatile oil, herba Coriandri volatile oil, herba Ocimi volatile oil, and rhizoma Acori Calami volatile oil; the origanum oil, the schizonepeta volatile oil, the zedoary volatile oil, the coriander volatile oil, the basil volatile oil and the acorus calamus volatile oil are equal in parts.
The emulsifying thickener is preferably one or more of soybean phospholipid, lauroyl polyoxyethylene (12) glyceride, sorbitan trioleate (span 85), egg yolk lecithin, and aluminum distearate.
The antioxidant is preferably one or more of butylated hydroxyanisole, dibutyl hydroxy toluene, vitamin E and ascorbyl palmitate.
A method for preparing a mucosa protective agent for artificial insemination of livestock is characterized by comprising the following steps:
SS 1: weighing 90-150 parts of matrix according to the proportion, adding the matrix into a liquid preparation tank, respectively adding 0.1-0.5 part of antioxidant and 1-30 parts of emulsifying thickener, heating and stirring until the mixture is completely dissolved to obtain a mixed solution 1;
SS 2: adding 1-15 parts of bacteriostatic agent into the mixed solution 1, and stirring until the mixture is uniform and stable to obtain a mixed solution 2;
SS 3: and filtering the mixed solution 2, cooling the filtered solution to room temperature, filling and sterilizing to obtain the mucosa protective agent for artificial insemination of livestock.
The invention is liquid at normal temperature, has certain viscosity, can be conveniently poured into the vagina and cervix of female livestock and adhered to the vagina and cervix mucosa, and plays roles of lubricating, inhibiting bacteria and protecting the mucosa when artificial fertilization is carried out. The raw materials of the invention are all medicinal grade auxiliary materials, have no toxic or side effect and no irritation, can not cause the property change of the artificial insemination semen, can not influence the activity of the sperm, has certain antibacterial ability, and can avoid the vaginal mucosa and cervix of the female livestock from being damaged and infected.
Detailed Description
The invention is further illustrated by the following examples:
example 1
A mucosa protective agent for livestock artificial insemination is characterized by comprising the following raw materials in parts by mass: 30 parts of medicinal white oil, 30 parts of medicinal liquid paraffin and 30 parts of medicinal ethyl oleate; 1 part of origanum oil, 1 part of schizonepeta volatile oil, 1 part of curcuma zedoary volatile oil, 1 part of coriander volatile oil, 1 part of basil volatile oil and 1 part of calamus volatile oil; 10 parts of lauroyl polyoxyethylene (12) glyceride and 10 parts of sorbitan trioleate (span 85); 0.2 part of vitamin E and 0.2 part of ascorbyl palmitate.
The preparation method of the schizonepeta volatile oil comprises the following steps: putting the schizonepeta herb into purified water with the mass 6 times of that of the schizonepeta herb, decocting for 1 hour, and decocting the filtered decoction dregs for 1 hour by using the purified water with the mass 4 times of that of the decoction dregs; mixing the filtrates, filtering, and concentrating to original medicinal materials: adding 95% ethanol 6 times the weight of the extract at a mass ratio of 1:1, stirring uniformly, precipitating with ethanol for 24 hours, filtering, collecting filtrate, recovering ethanol, filtering the obtained liquid medicine again, and adding purified water to dilute to 1: 1.
oregano oil, Curcumae rhizoma volatile oil, herba Coriandri volatile oil, herba Ocimi volatile oil, rhizoma Acori Calami volatile oil and herba Schizonepetae volatile oil by the same preparation method.
A method for preparing a mucosa protective agent for artificial insemination of livestock is characterized by comprising the following steps:
SS 1: weighing 30 parts of medicinal white oil, 30 parts of medicinal soybean oil and 30 parts of medicinal ethyl oleate according to a ratio, adding into a liquid preparation tank, and respectively adding 10 parts of lauroyl polyoxyethylene (12) glyceride and 10 parts of sorbitan trioleate (span 85); 0.2 part of vitamin E and 0.2 part of ascorbyl palmitate are heated and stirred until the vitamin E and the ascorbyl palmitate are completely dissolved to obtain a mixed solution 1;
SS 2: 1 part of origanum oil, 1 part of schizonepeta volatile oil, 1 part of curcuma zedoary volatile oil, 1 part of coriander volatile oil, 1 part of basil volatile oil and 1 part of calamus volatile oil; adding the mixture into the mixed solution 1, and stirring the mixture until the mixture is uniform and stable to obtain a mixed solution 2;
SS 3: and filtering the mixed solution 2, cooling the filtered solution to room temperature, filling and sterilizing to obtain the mucosa protective agent for artificial insemination of livestock.
Example 2
A mucosa protective agent for livestock artificial insemination is characterized by comprising the following raw materials in parts by mass: 30 parts of medicinal white oil, 30 parts of medicinal liquid paraffin and 30 parts of medicinal ethyl oleate; 1 part of origanum oil, 1 part of schizonepeta volatile oil, 1 part of curcuma zedoary volatile oil, 1 part of coriander volatile oil, 1 part of basil volatile oil and 1 part of calamus volatile oil; 10 parts of soybean lecithin and 10 parts of egg yolk lecithin; 0.2 part of butyl hydroxy anisole and 0.2 part of dibutyl hydroxy toluene.
The preparation method is the same as that of example 1.
Example 3
A mucosa protective agent for livestock artificial insemination is characterized by comprising the following raw materials in parts by mass: 30 parts of medicinal white oil, 30 parts of medicinal liquid paraffin and 30 parts of medicinal ethyl oleate; 1 part of origanum oil, 1 part of schizonepeta volatile oil, 1 part of curcuma zedoary volatile oil, 1 part of coriander volatile oil, 1 part of basil volatile oil and 1 part of calamus volatile oil; 20 parts of aluminum distearate; 0.4 part of ascorbyl palmitate.
The preparation method is the same as that of example 1.
Comparative example 1
A lubricant formulation was prepared in the same manner as in example 1, except that the formulation did not contain a base, and the other components and amounts were the same as in example 1.
Comparative example 2
A lubricating preparation is prepared by the same method as in example 1, except that the preparation does not contain a bacteriostatic agent, and other components and contents are the same as in example 1.
Comparative example 3
A lubricating formulation was prepared in the same manner as in example 1 except that the formulation did not contain an emulsifying thickener and the other components and amounts were the same as in example 1.
Comparative example 4
A lubricant formulation was prepared in the same manner as in example 1, except that the formulation did not contain an antioxidant, and the other components and amounts were the same as in example 1.
Comparative example 5
A lubricious formulation prepared in the same manner as in example 1, except that the base in the formulation did not contain a pharmaceutical grade white oil.
Comparative example 6
A lubricious formulation prepared in the same manner as in example 1, except that the base in the formulation did not contain pharmaceutical grade white oil, pharmaceutical grade liquid paraffin.
Comparative example 7
A lubricating formulation prepared according to the same method as in example 1, except that the bacteriostatic agent in the formulation does not contain catmint volatile oil.
Comparative example 8
A lubricant preparation is prepared in the same manner as in example 1, except that the bacteriostatic agent in the preparation does not contain oregano oil, catnip volatile oil, and zedoary volatile oil.
The sows to be bred are randomly selected 330, and randomly divided into 11 groups (A group, B group, C group, D group, E group, F group, G group, H group, I group, J group and K group), each of which has 30 heads. Group a is a blank control without any lubricant; group B used the lubricant in CN201610947042.5 example 1; group C was treated using example 1. D. E, F, G, H, I, J, K groups were treated with the present invention, comparative examples 1-8, respectively, blank groups were directly dosed using a syringe, and group B was applied by painting on the syringe prior to dosing. The using method of other groups is that after the private parts of the livestock are disinfected, the livestock are inserted into the vagina by a perfusion apparatus with a long and thin neck and then are squeezed and perfused into the vagina of the livestock.
TABLE 1 sow mating success rate and ratio of vaginal inflammation after 21 days of artificial insemination
| Group of | Success/head of mating | Visible inflammation occurrence/head | Success rate of mating | Incidence of inflammation |
| A | 20 | 14 | 66.67% | 46.67% |
| B | 22 | 7 | 73.33% | 23.33% |
| C | 30 | 0 | 100% | 0% |
| D | 26 | 5 | 86.67% | 16.67% |
| E | 25 | 6 | 83.33% | 20.00% |
| F | 28 | 4 | 93.33% | 13.33% |
| G | 28 | 2 | 93.33% | 6.67% |
| H | 27 | 3 | 90.00% | 10.00% |
| I | 25 | 2 | 80.00% | 6.67% |
| J | 26 | 2 | 86.67% | 6.67% |
| K | 28 | 4 | 93.33% | 13.33% |
As can be seen from Table 1, the incidence of inflammation was greatly reduced and the success rate of mating was greatly improved in group C of the mucosal protective agent for artificial insemination of livestock of the present invention, compared to the remaining groups. Compared with the group B in the prior art, the success rate of mating is obviously greatly improved. From D, E, F, G, when none of the formulations was present, the success of mating was reduced and the incidence of inflammation was increased compared to group C of the present invention. It is clear from the comparison of group H, I, J, K with group C of the present invention that the absence of a single agent in both the matrix and the bacteriostatic agent has an effect on the efficacy of the present invention, with the greater the absence of agent in the matrix and the bacteriostatic agent, the poorer the efficacy. The components of the invention have obvious influence on the curative effect, and the mucosa protective agent for the artificial insemination of the livestock prepared by the preferred components of the invention can obtain the best effect.
The above-described embodiments are preferred implementations of the present invention, and the present invention can be implemented in other ways without departing from the spirit of the present invention.
Claims (5)
1. A mucosa protective agent for livestock artificial insemination comprises the following raw materials in parts by mass: 90-150 parts of matrix, 1-15 parts of bacteriostatic agent, 1-30 parts of emulsifying thickener and 0.1-0.5 part of antioxidant, and is characterized in that the bacteriostatic agent comprises oregano oil, schizonepeta volatile oil, zedoary volatile oil, coriander volatile oil, basil volatile oil and calamus volatile oil in equal parts.
2. The mucosal protective agent for artificial insemination of domestic animals according to claim 1, wherein the base is preferably pharmaceutical grade white oil, pharmaceutical grade liquid paraffin, pharmaceutical grade ethyl oleate; the medicinal white oil, the medicinal liquid paraffin and the medicinal ethyl oleate are equal in parts.
3. Mucosal protectant for artificial insemination of livestock according to claim 1, said emulsifying thickener is preferably one or more of soybean phospholipids, lauroyl polyoxyethylene (12) glycerides, sorbitan trioleate (span 85), egg yolk lecithin, aluminium distearate.
4. Mucosal protectant for artificial insemination of livestock according to claim 1, said antioxidant is preferably one or more of butylated hydroxyanisole, butylated hydroxytoluene, vitamin E, ascorbyl palmitate.
5. A method for preparing a mucosa protective agent for artificial insemination of livestock is characterized by comprising the following steps:
SS 1: weighing 90-150 parts of matrix according to the proportion, adding the matrix into a liquid preparation tank, respectively adding 0.1-0.5 part of antioxidant and 1-30 parts of emulsifying thickener, heating and stirring until the mixture is completely dissolved to obtain a mixed solution 1;
SS 2: adding 1-15 parts of bacteriostatic agent into the mixed solution 1, and stirring until the mixture is uniform and stable to obtain a mixed solution 2;
SS 3: and filtering the mixed solution 2, cooling the filtered solution to room temperature, filling and sterilizing to obtain the mucosa protective agent for artificial insemination of livestock.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910059088.7A CN109453439B (en) | 2019-01-22 | 2019-01-22 | Mucous membrane protective agent for artificial insemination of livestock and preparation method thereof |
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| CN109453439B true CN109453439B (en) | 2021-05-14 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583128A (en) * | 2004-05-27 | 2005-02-23 | 梁伟 | Aromatic turmeric oil and its composite vaginal adhered cream and preparation thereof |
| CN1803185A (en) * | 2005-01-12 | 2006-07-19 | 中南大学湘雅二医院 | Compound zedoary turmeric oil gelata and its preparing method |
| CN104146957A (en) * | 2014-07-24 | 2014-11-19 | 西安力邦肇新生物科技有限公司 | Submicroemulsion antifungal drug spray preparation |
| CN104958788A (en) * | 2015-06-30 | 2015-10-07 | 苏州乔纳森新材料科技有限公司 | Medical lubricant and preparing method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201538181A (en) * | 2014-03-19 | 2015-10-16 | Pures Biotech Co Ltd | A semi-fluid composition for lubricating, moisture retaining, disinfecting and sterilizing |
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- 2019-01-22 CN CN201910059088.7A patent/CN109453439B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1583128A (en) * | 2004-05-27 | 2005-02-23 | 梁伟 | Aromatic turmeric oil and its composite vaginal adhered cream and preparation thereof |
| CN1803185A (en) * | 2005-01-12 | 2006-07-19 | 中南大学湘雅二医院 | Compound zedoary turmeric oil gelata and its preparing method |
| CN104146957A (en) * | 2014-07-24 | 2014-11-19 | 西安力邦肇新生物科技有限公司 | Submicroemulsion antifungal drug spray preparation |
| CN104958788A (en) * | 2015-06-30 | 2015-10-07 | 苏州乔纳森新材料科技有限公司 | Medical lubricant and preparing method thereof |
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Inventor after: Zhang Yongkui Inventor after: Zhang Fenghua Inventor after: Xia Jianfeng Inventor before: Zhang Yongkui Inventor before: Zhang Fenghua Inventor before: Xia Jianfeng |