CN109453179A - Application of the margosine in preparation prevention and treatment fungal infection drug - Google Patents
Application of the margosine in preparation prevention and treatment fungal infection drug Download PDFInfo
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- CN109453179A CN109453179A CN201811442872.8A CN201811442872A CN109453179A CN 109453179 A CN109453179 A CN 109453179A CN 201811442872 A CN201811442872 A CN 201811442872A CN 109453179 A CN109453179 A CN 109453179A
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- margosine
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- trichophyton rubrum
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- 239000003814 drug Substances 0.000 title claims abstract description 24
- 229940079593 drug Drugs 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 230000002265 prevention Effects 0.000 title claims abstract description 14
- 206010017533 Fungal infection Diseases 0.000 title abstract description 6
- 208000031888 Mycoses Diseases 0.000 title abstract description 6
- 241000223229 Trichophyton rubrum Species 0.000 claims abstract description 26
- 241000555688 Malassezia furfur Species 0.000 claims abstract description 18
- 206010016936 Folliculitis Diseases 0.000 claims abstract description 12
- 208000002474 Tinea Diseases 0.000 claims abstract description 11
- 241000893966 Trichophyton verrucosum Species 0.000 claims abstract description 9
- 206010043866 Tinea capitis Diseases 0.000 claims abstract description 5
- 206010067197 Tinea manuum Diseases 0.000 claims abstract description 5
- 210000004761 scalp Anatomy 0.000 claims abstract description 5
- 208000009189 tinea favosa Diseases 0.000 claims abstract description 5
- 201000004647 tinea pedis Diseases 0.000 claims abstract description 5
- 239000000470 constituent Substances 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 abstract description 19
- 238000000034 method Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 7
- 230000003385 bacteriostatic effect Effects 0.000 abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 6
- 230000036541 health Effects 0.000 abstract description 3
- 230000001681 protective effect Effects 0.000 abstract description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 20
- 239000000725 suspension Substances 0.000 description 10
- 230000001954 sterilising effect Effects 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 235000013399 edible fruits Nutrition 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 244000237986 Melia azadirachta Species 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 235000013500 Melia azadirachta Nutrition 0.000 description 5
- 240000000233 Melia azedarach Species 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- VBICKXHEKHSIBG-UHFFFAOYSA-N 1-monostearoylglycerol Chemical compound CCCCCCCCCCCCCCCCCC(=O)OCC(O)CO VBICKXHEKHSIBG-UHFFFAOYSA-N 0.000 description 2
- 206010063409 Acarodermatitis Diseases 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 229920001214 Polysorbate 60 Polymers 0.000 description 2
- 241000447727 Scabies Species 0.000 description 2
- 241000130764 Tinea Species 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 239000002917 insecticide Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000004006 olive oil Substances 0.000 description 2
- 235000008390 olive oil Nutrition 0.000 description 2
- 244000045947 parasite Species 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000004080 punching Methods 0.000 description 2
- 208000005687 scabies Diseases 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 239000012224 working solution Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 206010002368 Anger Diseases 0.000 description 1
- 241000244203 Caenorhabditis elegans Species 0.000 description 1
- 241000498255 Enterobius vermicularis Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010019909 Hernia Diseases 0.000 description 1
- 241000158728 Meliaceae Species 0.000 description 1
- 208000030852 Parasitic disease Diseases 0.000 description 1
- 206010034464 Periarthritis Diseases 0.000 description 1
- NAHTXVIXCMUDLF-DUJPAFNYSA-N Toosendanin Natural products O=C(O[C@H]1C(=O)[C@H]2[C@@](C)([C@H](O)C[C@H]3[C@@]4(C)[C@H](O)OC[C@@]23[C@@H](O)C[C@H]4OC(=O)C)[C@@]23[C@]1(C)[C@H](c1cocc1)C[C@H]2O3)C NAHTXVIXCMUDLF-DUJPAFNYSA-N 0.000 description 1
- UELITFHSCLAHKR-UHFFFAOYSA-N acibenzolar-S-methyl Chemical compound CSC(=O)C1=CC=CC2=C1SN=N2 UELITFHSCLAHKR-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- TUFPZQHDPZYIEX-UHFFFAOYSA-N alpha-Santonin Natural products C1CC2(C)C=CC(=O)C=C2C2C1C(C)C(=O)O2 TUFPZQHDPZYIEX-UHFFFAOYSA-N 0.000 description 1
- XJHDMGJURBVLLE-BOCCBSBMSA-N alpha-santonin Chemical compound C([C@]1(C)CC2)=CC(=O)C(C)=C1[C@@H]1[C@@H]2[C@H](C)C(=O)O1 XJHDMGJURBVLLE-BOCCBSBMSA-N 0.000 description 1
- 230000001741 anti-phlogistic effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 206010014881 enterobiasis Diseases 0.000 description 1
- 208000010824 fish disease Diseases 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- -1 furans triterpene compound Chemical class 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 229940074353 santonin Drugs 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- NAHTXVIXCMUDLF-RFNFAWMESA-N toosendanin Chemical compound C=1([C@H]2[C@]3(C)[C@@H](OC(C)=O)C(=O)[C@H]4[C@@]([C@@]53O[C@@H]5C2)(C)[C@H](O)C[C@H]2[C@@]3(C)[C@H](O)OC[C@]24[C@@H](O)C[C@H]3OC(=O)C)C=COC=1 NAHTXVIXCMUDLF-RFNFAWMESA-N 0.000 description 1
- 239000008488 toosendanin Substances 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
- A61K31/58—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids containing heterocyclic rings, e.g. danazol, stanozolol, pancuronium or digitogenin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
Landscapes
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses application of the margosine in preparation prevention and treatment fungal infection drug, specially application of the margosine in the drug for the ringworm that preparation prevention and treatment pityrosporum folliculitis or Trichophyton rubrum are led to.Bacteriostatic experiment method is punched by margosine pre-add bacterium solution pour plate, it proves that the margosine of 10.0mg/g content can be treated effectively and caused pityrosporum folliculitis is infected by Malassezia furfur (Malassezia furfur) CMCC (F) Y17, or the tinea manuum, tinea pedis, favus of the scalp as caused by Trichophyton rubrum (Trichophyton rubrum) CMCC (F) Tla etc., effect is very ideal, it is not only environmentally protective, and do not influence human health.
Description
Technical field
The present invention relates to natural medicine technical fields, and in particular to margosine answering in preparation prevention and treatment fungal infection drug
With.
Background technique
Have in bactericidal insecticidal activity object Quality Research from searching in natural plants, it has been found that the plant of about 1600 kinds of the whole world
Object has excellent bactericidal insecticidal activity, and wherein most study is Meliaceae plant, mainly China tree, melia toosendan and print chinaberry.It is this
Trees are rarely found infested, it innately carries biocidal efficacies, not only beautify the environment, but also have and lay the dust, is antibacterial, purification air.
According to Compendium of Material Medica record China tree as medicine using have effects that it is clearing heat and detoxicating, go irascibility, relieve pain, control hernia, scabies, expelling parasite.
China tree also has very high activity to fungi, agricultural pests, easily decomposes in a natural environment, safe and harmless to people, animal.In previous generation
It records the fifties, it is very popular research topic that Chinese scholar finds insecticide active substance from plant kingdom, the change about China tree
It studies point, the research of pharmacology, sterilization and disinsection effect etc., is especially to realize pest and disease damage integrated management in agriculture field, as
Non-harmful bion insecticide, research achievement are noticeable.Early stage people utilize the branches and leaves and fruit of margosa, as system
Make the raw material of soap, there is good bactericidal antiphlogistic effect, rural area Zeng Youyong neem fruit controls the example of child pinworm.Even if existing
The present, China tree are still the object of natural convenient pesticide-germicide first choice, and viral disease, bacillary disease often occur for freshwater fish culturing
Disease, parasitic disease, the usual control method of raiser is to launch neem fruit to Cultivated water, by neem fruit long period of soaking in fish
In the pool, the effect of playing preventing and curing of fish disease.Neem fruit can also be used to treat various fungal infection skin diseases (such as tinea, scabies),
Neem fruit or azedarach are commonly used in the Chinese medicine preparation of human body external application.Traditional Chinese Medicine Research Institute, Sichuan Province is being sought since nineteen fifty-three
When looking for instead of import drug santonin expeling roundworm, according to the record of Ancient Times in China document, traditional medicine and civil folk prescription, from several chinaberrys
A kind of drive ascarid effective component is had found in section's plant bark, through separation, is extracted and name as margosine (Toosen-danin), it should
Substance is furans triterpene compound.The margosine extracted from China tree, have expelling parasite desinsection, restraining and sterilizing bacteria, it is antiviral,
Antitumor activity, margosine easily decomposes in a natural environment, safe and harmless to people, animal.
Currently without about margosine in preparation prevention and treatment pityrosporum folliculitis or the ringworm that is led to of Trichophyton rubrum
The relevant report of application in drug.
Summary of the invention
The object of the present invention is to provide application of the margosine in preparation prevention and treatment fungal infection drug, specially margosine exists
Application in the drug for the ringworm that preparation prevention and treatment pityrosporum folliculitis or Trichophyton rubrum are led to.
The present invention punches bacteriostatic experiment method by margosine pre-add bacterium solution pour plate, it was demonstrated that the hardship of 10.0mg/g content
Chinaberry element, which is effectively treated, infects caused pityrosporum furfur hair by Malassezia furfur (Malassezia furfur) CMCC (F) Y17
Capsulitis, or the tinea manuum, tinea pedis, favus of the scalp as caused by Trichophyton rubrum (Trichophyton rubrum) CMCC (F) Tla etc., effect
Fruit is very ideal, not only environmentally protective, and does not influence human health.
Therefore, one of the object of the invention is to provide margosine answering in preparation prevention and treatment pityrosporum folliculitis drug
With.The pityrosporum folliculitis is pityrosporum folliculitis caused by Malassezia furfur CMCC (F) Y17 infects.
A kind of drug for preventing and treating pityrosporum folliculitis, includes margosine as active constituent.
Another object of the present invention is to provide the drug for the ringworm that margosine is led in preparation prevention and treatment Trichophyton rubrum
In application.The Trichophyton rubrum is Trichophyton rubrum CMCC (F) Tla；The ringworm includes the tinea manuum, tinea pedis or favus of the scalp
Deng.
A kind of drug for the ringworm that prevention and treatment Trichophyton rubrum is led to, includes margosine as active constituent.
The margosine pre-add bacterium solution pour plate punches bacteriostatic experiment method, comprising the following steps:
(1) Leeming&Notman culture medium (every liter contain peptone 10g, glucose 5g, yeast extract 10g, glycerol 1g,
Glycerin monostearate 0.5g, Tween-60 0.5g, olive oil 20g, agar powder 20g, solvent are water) preparation and sterilizing；
(2) cultured Malassezia furfur CMCC (F) Y17 and Trichophyton rubrum CMCC (F) Tla is respectively prepared
Certain density suspension takes suspension described in 1mL into sterilized culture dish, injection 20mL Leeming&Notman training
Base is supported, culture dish is gently shaken, mixes culture medium and suspension, cooling is stood, entreats and punch in plate after culture medium solidification；
(3) by margosine with ethanol water (volume ratio 1:1) be configured to 1.0mg/g containing margosine, 3.0mg/g,
The sample of tetra- concentration gradients of 5.0mg/g, 10.0mg/g injects 50 μ L margosine solution examples in the hole in step (2)；
(4) each concentration of margosine is done 3 times in parallel by the method for step (3), with ethanol water (volume ratio 1:1) work
For blank control；
(5) plate for containing Malassezia furfur CMCC (F) Y17 is cultivated into 72h in 35 DEG C of constant incubators, containing red hair
The plate of tinea bacterium CMCC (F) Tla cultivates 72h in 30 DEG C of constant incubators, observes antibacterial region, and measured and calculated with ruler
Clear antibacterial region overall width.
Wherein, the calculation formula of clear antibacterial region overall width are as follows: W=(T-D)/2 (the clear antibacterial region overall width of W=；
The hole T=and antibacterial area's overall width；The width in the hole D=)
As a result obtain: the margosine of 10.0mg/g concentration is to Malassezia furfur CMCC (F) Y17, Trichophyton rubrum CMCC
(F) the clear antibacterial region of Tla is larger.
It is preferred that sterilizing described in above-mentioned steps (1) is that high temperature and high pressure steam sterilizes, and 121 DEG C of sterilising temp, sterilization time
20min。
It is preferred that in above-mentioned steps (2) Malassezia furfur CMCC (F) Y17 and Trichophyton rubrum CMCC (F) Tla suspension
Liquid concentration is in OD600=1.0.
Compared with prior art, present invention has an advantage that
The present invention punches bacteriostatic experiment method by margosine pre-add bacterium solution pour plate, it was demonstrated that the hardship of 10.0mg/g content
Chinaberry element, which can be treated effectively, infects caused pityrosporum furfur by Malassezia furfur (Malassezia furfur) CMCC (F) Y17
Epifolliculitis, or the tinea manuum, tinea pedis, favus of the scalp as caused by Trichophyton rubrum (Trichophyton rubrum) CMCC (F) Tla etc.,
Effect is very ideal, not only environmentally protective, and does not influence human health.
Two plants of bacterium are purchased from Chinese medicine bacteria culture preservation administrative center, and the deposit number of Malassezia furfur is
CMCC (F) Y17 and the deposit number of Trichophyton rubrum are CMCC (F) Tla.
Specific embodiment
The following examples are further illustrations of the invention, rather than limiting the invention.
Pre-add bacterium solution pour plate punching it is antibacterial it is around-France be constantly dissolve using bacteriostatic agent through AGP test formed difference it is dense
Gradient is spent, to show its bacteriostasis, the clear antibacterial ring size size formed around through hole is to judge its bacteriostasis.Pre-add bacterium
The punching bacteriostatic experiment method operation of liquid pour plate is convenient, result is accurate and reliable, and the inhibition zone quality of acquisition is high, reproducible,
The uniformity, homogeneity of bacterium colony in test panel can be effectively ensured, cell concentration is in OD600=1.0 in test panel, plate
Bacterium colony concentration is fine and close, and inhibition zone boundary is obvious, is the suitable cell concentration for carrying out inhibition zone test.
Use following abbreviation in the present specification: CMCC is the abbreviation of Chinese medicine bacteria culture preservation administrative center, F generation
Table fungi, Y17, Tla are bacterium numbering, and the unit of temperature is degree Celsius (DEG C), min=minutes, h=hours, and mL=milliliters, mm
=millimeter, mg=milligrams, g=grams, OD600Refer to light absorption value of the solution at 600nm wavelength, percentage is in mass.
Embodiment 1
(1) Leeming&Notman culture medium is prepared and sterilizes: weighing peptone 10g, glucose 5g, yeast extract respectively
10g, glycerol 1g, glycerin monostearate 0.5g, Tween-60 0.5g, olive oil 20g, agar powder 20g, mixing, are dissolved in
1000mL water is stirred to being completely dissolved, then progress high temperature and high pressure steam sterilizing, 121 DEG C of sterilising temp, sterilization time 20min.
(2) it after Malassezia furfur CMCC (F) Y17 cultivates 72h in 35 DEG C of constant incubators, is diluted to sterile water
OD600=1.0 are configured to bacteria suspension working solution；After Trichophyton rubrum CMCC (F) Tla cultivates 72h in 30 DEG C of constant incubators,
OD is diluted to sterile water600=1.0 are configured to bacteria suspension working solution.
(3) taking 1mL Malassezia furfur CMCC (F) Y17 bacteria suspension to sterilized diameter is 90mm plate, is then poured into
20mL Leeming&Notman culture medium, gently shakes culture dish, mixes culture medium and suspension, stands cooled and solidified.
(4) taking 1mL Trichophyton rubrum CMCC (F) Tla bacteria suspension to sterilized diameter is 90mm plate, is then poured into
20mLLeeming&Notman culture medium, gently shakes culture dish, mixes culture medium and suspension, stands cooled and solidified.
(5) it is punched in (3) or (4) test panel with sterilized punch, the diameter in hole is 5.0mm.
(6) by be used for test margosine with ethanol water (volume ratio 1:1) be configured to 1.0mg/g, 3.0mg/g,
Tetra- concentration gradients of 5.0mg/g, 10.0mg/g inject 50 μ L margosine solution examples respectively in the hole in (5).
(7) each concentration does 3 in parallel, does blank control with ethanol water (ethyl alcohol and water volume ratio 1:1).
(8) it cultivates, observe, measurement: the plate of Malassezia furfur CMCC (F) Y17 will be contained in 37 DEG C of constant incubators
72h is cultivated, the plate of CMCC containing Trichophyton rubrum (F) Tla cultivates 72h in 30 DEG C of constant incubators, observes antibacterial region, and
It is measured with ruler and calculates clear antibacterial region overall width.
Wherein, the calculation formula of clear antibacterial region overall width are as follows: W=(T-D)/2 (the clear antibacterial region overall width of W=；
The hole T=and antibacterial area's overall width；The width in the hole D=)
As a result obtain: the margosine of 10.0mg/g content is to Malassezia furfur CMCC (F) Y17 and Trichophyton rubrum CMCC
(F) the clear antibacterial region that Tla is generated is bigger, and the clear antibacterial width to Malassezia furfur CMCC (F) Y17 is 2.9mm,
Clear antibacterial width to Trichophyton rubrum CMCC (F) Tla is 3.0mm, is specifically shown in Table 1.
The pre-add bacterium solution pour plate of 1 margosine of table punches bacteriostatic experiment result
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
It also should be regarded as protection scope of the present invention into retouching.
Claims (7)
1. application of the margosine in preparation prevention and treatment pityrosporum folliculitis drug.
2. application according to claim 1, which is characterized in that the pityrosporum folliculitis is Malassezia furfur
Pityrosporum folliculitis caused by CMCC (F) Y17 infects.
3. a kind of drug for preventing and treating pityrosporum folliculitis, which is characterized in that include margosine as active constituent.
4. application of the margosine in the drug for the ringworm that preparation prevention and treatment Trichophyton rubrum is led to.
5. application according to claim 4, which is characterized in that the Trichophyton rubrum is Trichophyton rubrum CMCC (F)
Tla。
6. application according to claim 4 or 5, which is characterized in that the ringworm includes the tinea manuum, tinea pedis or favus of the scalp.
7. a kind of drug for the ringworm that prevention and treatment Trichophyton rubrum is led to, which is characterized in that include margosine as active constituent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811442872.8A CN109453179B (en) | 2018-11-29 | 2018-11-29 | Application of toosendanin in preparing medicine for preventing and treating fungal infection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811442872.8A CN109453179B (en) | 2018-11-29 | 2018-11-29 | Application of toosendanin in preparing medicine for preventing and treating fungal infection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109453179A true CN109453179A (en) | 2019-03-12 |
| CN109453179B CN109453179B (en) | 2020-11-27 |
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| Application Number | Title | Priority Date | Filing Date |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1318406A (en) * | 2001-01-17 | 2001-10-24 | 云南省农业科学院生物技术研究所 | Veterinary liniment containing azedarachin and its preparation |
| CN101040940A (en) * | 2007-04-25 | 2007-09-26 | 内蒙古医学院 | Sanzi liquid for washing |
-
2018
- 2018-11-29 CN CN201811442872.8A patent/CN109453179B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1318406A (en) * | 2001-01-17 | 2001-10-24 | 云南省农业科学院生物技术研究所 | Veterinary liniment containing azedarachin and its preparation |
| CN101040940A (en) * | 2007-04-25 | 2007-09-26 | 内蒙古医学院 | Sanzi liquid for washing |
Non-Patent Citations (2)
| Title |
|---|
| 梅全喜: "《现代中药药理与临床应用手册 第3版》", 31 October 2016, 中国中医药出版社 * |
| 钟鸣等: "《中国壮药学》", 31 May 2016, 广西民族出版社 * |
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| CN109453179B (en) | 2020-11-27 |
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