KR20070063936A - Scuticociliates insecticide composition using butyraldehyde - Google Patents

Abstract

An insecticide composition for Scuticociliates comprising butyraldehyde is provided to improve insecticidal effect of the composition by using butyraldehyde which is not used as Scuticociliates insecticide previously, and inhibit damage of fishes caused by toxicity of insecticides by mixing butyraldehyde with organic solvent and diluting them with water. The insecticide composition of Scuticociliates comprising butyraldehyde is prepared by mixing butyraldehyde with organic solvent, and diluting the mixture with water, wherein the organic solvent is ethanol, methanol, ether, acetone, benzene or toluene; the mixing volume ratio of butyraldehyde to organic solvent is 1:0.1-1, and the mixing volume ratio of the mixture to water is 1:0.1-2; and the composition is added into seawater in a fish farm in the amount of 0.2-0.9 liter per 1 ton.

Description

Composition for insecticide insecticides using butylaldehyde {SCUTICOCILIATES INSECTICIDE COMPOSITION USING BUTYRALDEHYDE}

1 is a manufacturing process diagram of the insecticidal insecticidal composition using butylaldehyde of the present invention.

Figure 2: Photomicrographs after Scutica insect culture.

Figure 3: Scutika insect cytoplasm breakdown generated when using the composition for insecticide Scutika insecticidal of the present invention.

The present invention relates to a composition for treating insecticidal insecticidal using butylaldehyde.

Parasites of farmed fish include Ichthyophthirius multifillis, Cryptocaryon irritans, Trichodina, Chilodonella cyprini, Ambiphrya, Amphiphroma, and Epimpoma. Epistylis, Carchesium, and Scuticociliads.

Among them, Scutika is a ciliated insect of 30 to 40 μm, and frequently occurs in artificial seedlings and fish of the flounder.

Scutika insects have been reported to cause a variety of fish body surface, fins and kidneys, blood vessels and brain tissues, and most of them have been reported to kill flounder through destruction of plastic connective tissue.

In addition, unlike the parasites of other fish, it is difficult to treat during infection because the parasite is invaded through the blood vessels through the blood vessels.

Scotica worms are also detected in rotifers, the prey organisms for flounder, and are parasitic from fry to sea urchins.

Formalin was used until recently in flounder fish farms for the insecticide of Scutika.

However, due to the toxic component of formalin is prohibited, and to replace it, hydrogen peroxide, stabilized chlorine, etc., in addition to formalin, are used for in vitro parasite control, but have little effect.

Therefore, in recent years, as a way to solve this problem, a study on the insecticide Skutika insects using weak toxic components have been made.

Korean Patent Publication No. 10-2005-0045714 (Skutika disease preventive treatment of aquaculture fish containing terpena hydrochloride as an active ingredient), using a drug containing terpena hydrochloric acid, using Prophylactic and therapeutic effects are disclosed.

Korean Registered Patent Publication No. 10-0279281 (Fish Parasite Scutica Insecticide with Alginate Oligosaccharide Derivatives) includes low molecular weight of alginate as a polymer, preparation of oligosaccharide alginate, development of fish parasite Scutica insecticide and electric insecticide. Disclosed is a manufacturing method.

Korean Patent Laid-Open No. 10-0379026 (Method for Controlling Scutika Insects of Cultured Flounder) discloses a method for controlling Scutica insects infected inside and outside of cultured flounder using ketoconazole.

However, the above inventions are difficult to manufacture insecticides, and are not suitable in terms of economics, and the oral administration method used as a method for controlling Scutica insects is practically difficult to use.

In addition, due to the toxicity of the components used as pesticides there is a problem that the fish pass and fish toxicity occurs.

The present invention is to solve the above problems, it is an object to apply the butyl aldehyde, which has not been used conventionally for the scutica insects as a scutica insect insecticidal composition.

In particular, there is also an object to improve the insecticidal insecticidal effect by applying butylaldehyde as a composition for insecticidal insecticidal.

In addition, there is also an object to provide a composition for insecticide insecticide Scutika that can control the infected fish by a simple bathing method.

The present invention relates to a composition for treating insecticidal insecticidal using butylaldehyde.

In the composition for the insecticide insecticide insecticide using butylaldehyde (butyraldehyde) of the present invention, after preparing a mixture by mixing butylaldehyde (butyraldehyde) and an organic solvent, the composition for insecticide insecticide insecticide prepared by diluting the mixture into water to be.

The inventors of the present invention, in order to develop a pesticidal composition for Scutica, butylaldehyde (Butyraldehyde), benzylaldehyde (Benzylaldehyde), cinnamic aldehyde (Cinnamic aldehyde), Eugenol, Salicylic aldehyde (Salicyl aldehydes, acetaldehyde, vanillin, camphor, campen, limonene, sinamyl acetate, sinoleyl, rotenone, hinoki Organics such as thiol (Hinokitiol), nicotinic acid, isobutyl aldehyde (Isobutyraldehyde), lauric acid, lactic acid, linoleic acid, undecylenic acid The pesticidal effect on Scutica insects was observed using the compound.

As a result, excellent insecticidal effect and no fish toxicity, economically suitable butylaldehyde (Butyraldehyde) was selected and used for the preparation of the insecticidal composition of the present invention.

Butylaldehyde is an organic compound composed of CH ₃ CH ₂ CH ₂ CHO. It is named after the butyric acid obtained by oxidizing aldehyde, but it is contained in animal fat such as butter. It is an organic compound that is found in many plant essential oils.

The organic solvent is a substance that dissolves the solute and is used to dissolve the butylaldehyde component.

As the organic solvent, one selected from ethanol, methanol, ether, acetone, benzene and toluene is used.

However, organic solvents are also toxic, so an appropriate amount should be used.

Water serves to adjust the concentration of the insecticidal composition prepared by mixing butylaldehyde and an organic solvent.

The inventors of the present invention have undergone a lot of trial and error to find a method that prevents fish death due to toxicity using the butylaldehyde and exhibits a high insecticidal effect against Scutika insects, and then, butylaldehyde ( butyraldehyde) and the organic solvent was mixed to prepare a mixture, it was found that when the mixture is prepared by diluting the water can be used as a composition for the insecticidal insecticides.

In particular, the organic solvent was 0.1 to 1 times compared to the volume of butyl aldehyde (butyraldehyde) when the mixture is prepared and used, there is no toxicity, it can be seen that exhibits a high pesticidal effect.

In addition, water is diluted 0.1 to 2 times the volume of the mixture of butyl aldehyde and the organic solvent, when diluted to prepare a pesticidal composition, it can be seen that the fish exhibits excellent insecticidal effect against Scutika insects without death. there was.

When using the insecticidal composition 0.2 ~ 0.9 L per 1 ton aquaculture water, it can be usefully used as an environmentally-friendly hygiene pesticides with environmental preservation and purification as non-toxic to natural enemies and constriction.

Referring to the method for producing a composition for insecticide insecticide Scutica using butylaldehyde of the present invention.

1. First step: preparing the mixture

Butylaldehyde (butyraldehyde) and the organic solvent is mixed to prepare a mixture.

As the organic solvent, one selected from ethanol, methanol, ether, acetone, benzene and toluene is used.

In this case, it is preferable to prepare a mixture by mixing the organic solvent 0.1 to 1 times the volume of butyl aldehyde (butyraldehyde).

2. Second step: Preparation of pesticide composition

Water was added to the mixture prepared in the first step and diluted to prepare a pesticidal composition.

At this time, if excessively diluted, the insecticidal effect is reduced.

In order to prevent this problem, it is preferable to dilute the amount of water 0.1 to 2 times the volume of the mixture.

Commonly used water is fresh water and seawater.

At this time, in order to prepare a pesticidal composition exhibiting a better insecticidal effect is used sterile seawater.

Sterilized seawater is collected by filtering the seawater coming in through the fish pond collection pipe, sterilized for 15 minutes at 121 ℃.

When the insecticidal composition diluted with sterile seawater is used in a bathing method, it also serves to prevent fish death by controlling osmotic pressure.

Hereinafter, the present invention is described in detail with reference to Examples and Experimental Examples for the insecticidal insecticidal composition using butylaldehyde, but these are not intended to limit the scope of the present invention.

Example 1 Preparation of Scutica insecticidal composition 1 using butylaldehyde of the present invention

98% butylaldehyde and 95% ethanol prepared from junsei were prepared.

300 mL of the prepared butylaldehyde and 200 mL of ethanol were mixed to prepare a mixture.

500 ml of water was added to the mixture and diluted to prepare a composition for treating insecticides.

Example 2 Preparation of Scutica Insecticidal Composition 2 Using Butylaldehyde of the Present Invention

98% butylaldehyde and 95% ethanol prepared from junsei were prepared.

800 ml of prepared butylaldehyde and 100 ml of ethanol were mixed to prepare a mixture.

100 ml of water was added to the mixture and diluted to prepare a composition for treating insecticides.

<Comparative Example 1> Composition for Scutica insect insecticide using formaldehyde

To prepare a pesticidal composition in the same manner as in Example 1 of the present invention,

Insecticidal compositions were prepared using 37.0% formaldehyde solution manufactured by JUNSEI instead of butylaldehyde.

<Comparative Example 2> Composition for Insecticidal Insecticidal Scutica using Cinamicaldehyde

To prepare a pesticidal composition in the same manner as in Example 1 of the present invention,

Insecticidal compositions were prepared using 99.0% Cinamic aldehyde manufactured by Alfa Aesar instead of butylaldehyde.

Comparative Example 3 Composition for Insecticidal Scutica Insecticide Using Eugenol

To prepare a pesticidal composition in the same manner as in Example 1 of the present invention,

Insecticidal compositions were prepared using 99.0% Eugenol manufactured by Aldrich instead of butylaldehyde.

Comparative Example 4 Composition for Insecticidal Insecticides Using Acetaldehyde

To prepare a pesticidal composition in the same manner as in Example 1 of the present invention,

Insecticidal compositions were prepared using 99.0% acetaldehyde prepared from Fluka instead of butylaldehyde.

<Comparative Example 5> composition for insecticide insecticide Scutica using camphor

To prepare a pesticidal composition in the same manner as in Example 1 of the present invention,

Insecticidal compositions were prepared using 90% camphor (Camphene) manufactured by Merck instead of butylaldehyde.

Experimental Example 1 Investigation of the Effect on Scutika Insects

1. Preparation of experimental materials

The compositions for the insecticidal insecticides of Examples 1 and 2 were prepared.

In comparison, 1 to 5 Scutica insecticidal insecticidal compositions were prepared.

2. Experimental method

1) Scutika insect culture

The isolates were cultured directly from the flounder diseased with Scutika insects.

The tissues of the infected site were partially removed from the flounder diseased fish, placed on a slide glass, and examined under a microscope. As a result, it was confirmed that Scutica was infected (FIG. 2).

The identified Scutica fungus was inoculated with P1Y1 (1% proteose peptone, 1% yeast extract) medium in water and cultured at 15 ° C.

There are many kinds of bacteria in the cultured Scutika insect culture, and the fish-coated cell line was used to purely cultivate Scutika insects.

At this time, the fish stem cell line CHSE-214 (Chinook salmon embryo) was added to 10% FBS and penicillin-streptomycin in MEM (Modified Eagle's Medium) medium at pH 7.3, and then incubated at 20 ° C. for 3 days. .

The cultured fish coin cell line was inoculated with the first cultured Scutica insect culture solution, and then purely cultured at 20 ° C. for 5 days.

2) Insecticide Insect Investigation

Using the hematocytometer, the number of proliferated squatica cells was counted to determine the number of squatica cells within 1 mm × 1 mm, and it was confirmed that 2.0 × 10 ⁴ cells per 1 ml of the pure culture medium were contained.

A total of 14 well plates were prepared by adding 5 ml of water to 5 ml of pure culture solution.

The compositions of Examples 1 and 2 and Comparative Examples 1, 2, 3, 4, and 5 were added to 2 well plates of 1.0 ml each, followed by stirring in a 20 ° C shaking incubator.

After stirring, the number of Scutica insects was measured every 1 hour, 2 hours, 6 hours, 12 hours, and 24 hours.

In the measurement, the culture solution was dropped on a slide glass, and then measured under a microscope to determine the number of normal individuals except the lysed individuals.

3. Experimental Results

As a result, it is shown in Table 1 below.

Table 1 Insecticidal insecticidal rate using the insecticidal composition of the present invention

Scutika insects Insecticide rate (%) Remarks 1 hours 2 hours 6 hours 12 hours 24 hours Example 1 2.9 × 10 ³ 4.5 × 10 ² 45 8 3 92 Example 2 1.5 × 10 ³ 2.3 × 10 ² 33 7 2 95 Comparative Example 1 1.0 × 10 ⁵ 1.0 × 10 ⁵ 1.0 × 10 ⁵ 1.0 × 10 ⁵ 1.0 × 10 ⁵ 0 Decreased mobility Comparative Example 2 1.0 × 10 ⁴ 3.2 × 10 ³ 9.0 × 10 50 30 90 Toxicity to fish Severe LC 50 <100 mg / L Comparative Example 3 1.0 × 10 ⁴ 2.8 × 10 ³ 1.3 × 10 ² 90 70 90 Toxicity to fish Severe LC 50 <100 mg / L Comparative Example 4 1.0 × 10 ⁵ 1.0 × 10 ⁵ 1.0 × 10 ⁵ 1.0 × 10 ⁵ 1.0 × 10 ⁵ 0 Shape change Comparative Example 5 1.1 × 10 ³ 2.4 × 10 0 0 0 100 Toxicity to fish Severe LC 50 <100 mg / L

As shown in Table 1, the insecticidal compositions of Comparative Examples 1 and 4 did not reduce the number of Scutika insects, only the effect of reducing the motility or change the shape of the Scutika insects.

In addition, in the insecticidal composition of Comparative Examples 2 and 3, the insecticidal effect was shown, but the effect was insufficient, and when LC ₅₀ <100 mg / L, severe fish toxicity was shown.

In the pesticidal composition of Comparative Example 5, the insecticidal effect was high, but showed severe fish toxicity when using LC ₅₀ <100 mg / L.

However, the compositions of Examples 1 and 2 showed a high pesticidal effect, but no toxicity.

It was confirmed in Figure 3 that the Scutica insect cytoplasm was destroyed by the insecticidal compositions of Examples 1 and 2.

Therefore, when using the insecticidal compositions of Examples 1 and 2 prepared by the present invention it was found that at least 90% of the insecticidal rate and no toxicity to Scutica insects.

Experimental Example 2 Toxicity Study on Fishes

1. Preparation of experimental materials

The compositions for the insecticidal insecticides of Examples 1 and 2 were prepared.

In comparison, 1 to 5 Scutica insecticidal insecticidal compositions were prepared.

As an experimental fish, flounder fry (5 cm) were prepared for each 100 tanks.

2. Experimental method

Put the flounder prepared with experimental fish into a round tank filled with 100 L of seawater, respectively, and put 500 ml of the insecticidal compositions of Examples 1 and 2 and the insecticidal compositions of Comparative Examples 1,2,3,4 and 5, respectively. Toxicity to flounder larvae was confirmed.

Toxicity was confirmed after 1 hour, 2 hours and 24 hours of bathing.

3. Experimental Results

The results are shown in Table 2 below.

<Table 1> Amount of flounder fry

1 hours 2 hours 24 hours Example 1 0 0 0 Example 2 0 0 0 Comparative Example 1 10 15 22 Comparative Example 2 27 35 69 Comparative Example 3 20 32 53 Comparative Example 4 2 4 10 Comparative Example 5 48 100 100

As shown in Table 2, when using the compositions of Examples 1 and 2, not one was killed.

However, in the compositions of Comparative Examples 2 and 3, the toxicity was high and the amount of abducted larvae was high. In the composition of Comparative Example 5, the toxicity was so strong after 2 hours of bathing that the abductions were all killed.

In addition, when using the compositions of Comparative Examples 1 and 4, the mortality was less than the compositions of Comparative Examples 1, 2, and 5, but showed a significant mortality over time, it is not suitable as a pesticidal composition. Could.

Therefore, when using the compositions of Examples 1 and 2 prepared by the present invention did not appear to be toxic to abduction fry, it was found that it is suitable as a pesticidal composition.

Experimental Example 3 Remedies for Scutica insects in Aquaculture

1. Preparation of experimental materials

The compositions for the insecticidal insecticides of Examples 1 and 2 were prepared.

In comparison, 1 to 5 Scutica insecticidal insecticidal compositions were prepared.

2. Experimental method

In the fish farm (10 pyeong) each contained 100 flounder fry, and at this time, five of the flounder fry in the fish farm was selected to confirm the infection, the epidermis and gills were infected with Scotchworm.

The fish farm contained 19.8 ton of seawater in the tank.

Therefore, 7 L of the compositions of Examples 1 and 2 and the compositions of Comparative Examples 1, 2, 3, 4, and 5 were placed in each fish farm.

Thereafter, 5 animals were sampled for 2 hours, 2 days, and 7 days, respectively, and the epidermal and gill portions were identified under a microscope.

3. Experimental Results

The results are shown in Table 3.

TABLE 1 Flounder fry control rate (%)

Scutika insecticidal rate (%) Flounder fry mortality rate (%) 2 hours 24 hours 1 day 7 days Example 1 100 100 0 0 Example 2 100 100 0 0 Comparative Example 1 5 7 30 100 Comparative Example 2 30 42 77 100 Comparative Example 3 32 47 59 100 Comparative Example 4 2 4 48 100 Comparative Example 5 90 95 100 100

As a result, when using the insecticidal compositions of Comparative Examples 1 and 4, the results of microscopic examination showed that the Scutica insects were almost eliminated, and the salvage effect of infected flounder larvae was hardly observed. there was.

In addition, when using the insecticidal compositions of Comparative Examples 2, 3 and 5, the results of microscopic examination showed that the Scutica insects were reduced, and the control effect of infected flounder fry was high, but the flounder fry died due to the toxicity of the composition. Could.

However, when using the compositions for the insecticidal insecticides prepared by Examples 1 and 2, the insects were completely removed, and there was no mortality of the flounder fry.

Therefore, halibut fry did not occur, and the Scutika insects were also completely removed and confirmed that the high salvage effect of infected halibut fry can be raised normally.

According to the present invention, by applying butylaldehyde, which has not been conventionally used for insecticide insecticides, as a composition for insecticide insecticides, an insecticidal composition for improving the insecticide insecticidal effect is provided.

In addition, there is provided a composition for insecticide insecticide Skutika which can control the infected fish by a simple bathing method.

Claims (4)

In the composition for insecticide insecticides, Butylaldehyde (butyraldehyde) and an organic solvent mixed to prepare a mixture, and then prepared by diluting the mixture with water, Scutica insecticidal composition using butylaldehyde. The method of claim 1, The organic solvent is characterized in that the mixed 0.1 to 1 times the volume of butyl aldehyde (butyraldehyde), Scutica insecticidal composition using butylaldehyde. The method of claim 1, The organic solvent is characterized in that one selected from ethanol, methanol, ether, acetone, benzene, toluene Scutica insecticidal composition using butylaldehyde. The method of claim 1, Water is diluted 0.1 to 2 times the volume of the mixture, characterized in that to dilute, Scutica insecticidal composition using butylaldehyde.

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