CN109432136A - 一种益生菌组合制剂及其制备方法和应用 - Google Patents
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Abstract
本发明公开了一种益生菌组合制剂及其制备方法和应用。其由下述重量份的原料制得:鼠李糖乳杆菌5‑15份,副凯氏乳酸杆菌8‑16份,植物乳杆菌2‑7份,发酵乳酸杆菌7‑15份,瑞士乳杆菌1‑8份,发酵乳杆菌10‑15份,干酪乳杆菌8‑17份,乳酸乳球菌12‑16份,唾液乳酸杆菌3‑8份,龙根菌7‑11份,LA‑5嗜酸乳杆菌3‑15份,BB‑12雷特氏B菌7‑15份,硅酸钙5‑8份,甘油4‑10份,阿拉伯胶4‑8份,壳聚糖1‑5份。本发明制备工艺简单,将菌泥和包埋保护剂混合均匀制得益生菌组合制剂,人体服用后,可以迅速发挥作用,促进消化吸收,有效治疗便秘。
Description
技术领域
本发明属于微生物制剂领域,涉及一种益生菌组合制剂及其制备方法和应用。
背景技术
益生菌是指投入后通过改善宿主肠道菌群生态平衡而发挥有益作用,达到提高宿主(人和动物)健康水平和健康状态的活菌制剂及其代谢产物。近年来,国内外研制出多种益生菌活菌制剂,其基本指导思想是用人或动物正常生理菌群的成员,经过选种和人工繁殖,通过各种途径和剂型制成活菌制剂,然后再以投入方式使其回到原来环境,发挥自然的生理作用。益生菌是活的微生物,当摄入足够数量时,对宿主起有益健康作用。
便秘是困扰大多数中老年人的小毛病,世界肠胃组织调查表明全球约有12%的人群有不同程度的便秘,居住于医疗机构的老年人超过50%患有便秘,然而就医率不足四分之一,在中国人们依赖中药来治疗便秘,这类中药中一般都存在能够引发腹泻的成分,比如大黄、番泻叶、决明子等,这就造成患者不是拉不出,就是拉肚子的状况;还有一些用物理疗法来治疗便秘的如灌肠等则给患者身体上带来痛苦的折磨。
目前市场上的益生菌产品层出不穷,但专门治疗便秘的产品很少,且大多数的益生菌产品是未经包埋处理的益生菌产品,例如酸奶、益生菌口服液或营养液、冻干菌粉等,由于人体的胃酸、胆汁酸有杀菌作用,这些益生菌进入人体后会失活,而不能发挥其理想的疗效。因此,研究出一种生产工艺简单,有效治疗便秘的益生菌组合物是本领域技术人员亟需解决的问题。
发明内容
本发明的目的是提供一种益生菌组合制剂及其制备方法和应用,可以有效治疗便秘,解决目前技术存在的缺陷和不足。
本发明解决上述技术问题的技术方案如下:
本发明提供一种益生菌组合制剂,其由下述重量份的原料制得:鼠李糖乳杆菌5-15份,副凯氏乳酸杆菌8-16份,植物乳杆菌2-7份,发酵乳酸杆菌7-15份,瑞士乳杆菌1-8份,发酵乳杆菌10-15份,干酪乳杆菌8-17份,乳酸乳球菌12-16份,唾液乳酸杆菌3-8份,龙根菌7-11份,LA-5嗜酸乳杆菌3-15份,BB-12雷特氏B菌7-15份,硅酸钙5-8份,甘油4-10份,阿拉伯胶4-8份,壳聚糖1-5份。
优选地,其由下述重量份的原料制得:鼠李糖乳杆菌8份,副凯氏乳酸杆菌10份,植物乳杆菌4份,发酵乳酸杆菌11份,瑞士乳杆菌5份,发酵乳杆菌13份,干酪乳杆菌12份,乳酸乳球菌14份,唾液乳酸杆菌5份,龙根菌7份,LA-5嗜酸乳杆菌8份,BB-12雷特氏B菌11份,硅酸钙6份,甘油4份,阿拉伯胶6份,壳聚糖3份。
一种益生菌组合制剂的制备方法,包括以下步骤:
(1)按上述的重量份称取各原料;
(2)将鼠李糖乳杆菌、副凯氏乳酸杆菌、植物乳杆菌、发酵乳酸杆菌、瑞士乳杆菌、发酵乳杆菌、干酪乳杆菌、乳酸乳球菌、唾液乳酸杆菌、龙根菌、LA-5嗜酸乳杆菌、BB-12雷特氏B菌用发酵罐进行混合培养,获得菌泥,备用;
(3)将硅酸钙、甘油、阿拉伯胶、壳聚糖混合搅拌均匀,加入步骤(2)获得的菌泥,均质,经低温冷冻干燥,得益生菌组合制剂;
(4)将步骤(3)得到的益生菌组合制剂用铝箔或棕色玻璃瓶包装。
进一步,所述步骤(2)中,培养温度为35~37℃,培养时间为12-48h。
进一步,所述步骤(2)中,获得菌泥中菌体总浓度为1.5×109-1.5×1012CFU/ml。
进一步,所述步骤(3)中,将硅酸钙、甘油、阿拉伯胶、壳聚糖混合,搅拌时间为20-30分钟。
进一步,所述步骤(3)中,加入菌泥后,均质时间为40-50分钟。
进一步,所述步骤(3)中,干燥至含水量为1%-3%。
一种益生菌组合制剂在食品工业、制药工业、健康保健工业或天然产品生产中的应用。
本发明的有益效果为:本发明以鼠李糖乳杆菌、副凯氏乳酸杆菌、植物乳杆菌、发酵乳酸杆菌、瑞士乳杆菌、发酵乳杆菌、干酪乳杆菌、乳酸乳球菌、唾液乳酸杆菌、龙根菌、LA-5嗜酸乳杆菌、BB-12雷特氏B菌12种益生菌作为菌泥,益生菌种类丰富,使用范围较广,适应更广泛的人群的不同肠道特征。本发明采用硅酸钙、甘油、阿拉伯胶、壳聚糖作为包埋保护剂包裹菌泥形成益生菌组合物,当使用者服用该复合益生菌组合物后,水渗透到硅酸钙内部,从而进入到益生菌中,休眠菌迅速复活,在益生元的存在下继续生长,繁殖,并随食物一道进入到人体的胃肠道,因此可以迅速发挥作用,快速解决人体便秘。本发明制备工艺简单,适于大规模化生产。
具体实施方式
以下的实施例在于详细说明本发明,只是本发明的较佳实施例,并非限制本发明。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。
实施例1
(1)称取鼠李糖乳杆菌8克,副凯氏乳酸杆菌10克,植物乳杆菌4克,发酵乳酸杆菌11克,瑞士乳杆菌5克,发酵乳杆菌13克,干酪乳杆菌12克,乳酸乳球菌14克,唾液乳酸杆菌5克,龙根菌7克,LA-5嗜酸乳杆菌8克,BB-12雷特氏B菌11克,硅酸钙6克,甘油4克,阿拉伯胶6克,壳聚糖3克。
(2)将鼠李糖乳杆菌、副凯氏乳酸杆菌、植物乳杆菌、发酵乳酸杆菌、瑞士乳杆菌、发酵乳杆菌、干酪乳杆菌、乳酸乳球菌、唾液乳酸杆菌、龙根菌、LA-5嗜酸乳杆菌、BB-12雷特氏B菌用发酵罐进行混合培养,培养温度为36℃,培养时间为24h。获得菌泥,备用,菌泥中菌体总浓度为1.5×1012CFU/ml;
(3)将硅酸钙、甘油、阿拉伯胶、壳聚糖混合搅拌30分钟,加入步骤(2)获得的菌泥,均质50分钟,经低温冷冻干燥至含水量为1%,得益生菌组合制剂;
(4)将上述得到的益生菌组合制剂用铝箔或棕色玻璃瓶包装。
实施例2
(1)称取鼠李糖乳杆菌5克,副凯氏乳酸杆菌8克,植物乳杆菌2克,发酵乳酸杆菌7克,瑞士乳杆菌1克,发酵乳杆菌10克,干酪乳杆菌8克,乳酸乳球菌12克,唾液乳酸杆菌3克,龙根菌7克,LA-5嗜酸乳杆菌3克,BB-12雷特氏B菌7克,硅酸钙5克,甘油4克,阿拉伯胶4克,壳聚糖1克。
(2)将鼠李糖乳杆菌、副凯氏乳酸杆菌、植物乳杆菌、发酵乳酸杆菌、瑞士乳杆菌、发酵乳杆菌、干酪乳杆菌、乳酸乳球菌、唾液乳酸杆菌、龙根菌、LA-5嗜酸乳杆菌、BB-12雷特氏B菌用发酵罐进行混合培养,培养温度为35℃,培养时间为12h。获得菌泥,备用,菌泥中菌体总浓度为1.5×109CFU/ml;
(3)将硅酸钙、甘油、阿拉伯胶、壳聚糖混合搅拌20分钟,加入步骤(2)获得的菌泥,均质40分钟,经低温冷冻干燥至含水量为1%,得益生菌组合制剂;
(4)将上述得到的益生菌组合制剂用铝箔或棕色玻璃瓶包装。
实施例3
(1)称取鼠李糖乳杆菌15克,副凯氏乳酸杆菌16克,植物乳杆菌7克,发酵乳酸杆菌15克,瑞士乳杆菌8克,发酵乳杆菌15克,干酪乳杆菌17克,乳酸乳球菌16克,唾液乳酸杆菌8克,龙根菌11克,LA-5嗜酸乳杆菌15克,BB-12雷特氏B菌15克,硅酸钙8克,甘油10克,阿拉伯胶8克,壳聚糖5克。
(2)将鼠李糖乳杆菌、副凯氏乳酸杆菌、植物乳杆菌、发酵乳酸杆菌、瑞士乳杆菌、发酵乳杆菌、干酪乳杆菌、乳酸乳球菌、唾液乳酸杆菌、龙根菌、LA-5嗜酸乳杆菌、BB-12雷特氏B菌用发酵罐进行混合培养,培养温度为37℃,培养时间为48h,获得菌泥,备用,菌泥中菌体总浓度为1.5×1012CFU/ml;
(3)将硅酸钙、甘油、阿拉伯胶、壳聚糖混合搅拌30分钟,加入步骤(2)获得的菌泥,均质50分钟,经低温冷冻干燥至含水量为3%,得益生菌组合制剂;
(4)将上述得到的益生菌组合制剂用铝箔或棕色玻璃瓶包装。
试验例
选取便秘患者250例,年龄从55岁到75岁,按照统计学原理将患者分为5组,各组患者无明显统计学差异。
设置对比例1,对比例1与实施例1相比较,只有原料中未添加龙根菌、LA-5嗜酸乳杆菌和BB-12雷特氏B菌,其他原料、质量及制备方法与实施例1完全相同。
第1组患者服用实施例1制备得到的益生菌组合制剂,第2组患者服用实施例2制备得到的益生菌组合制剂,第,3组患者服用实施例3制备得到的益生菌组合制剂,第4组患者服用对比例1制备得到的益生菌组合制剂,第5组患者不服用益生菌组合制剂作为空白对照组。服用剂量为2.0g/次,2次/天,疗程共15天;服用方法为直接吞服,服用期间忌辛辣刺激食物,并合理饮食,建立良好排便习惯。
疗效判定标准:
痊愈:大便正常,或恢复至病前水平,其他症状全部消失。
显效:便秘明显改善,间隔时间及便质接近正常;或大便稍干而排便时间在72小时以内,其他症状大部分消失;
有效:排便间隔时间缩短1天,或便质干结改善,其他症状均有好转。
无效:便秘及其他症状均无改善。
试验结果:具体见表1。
表1临床试验结果痊愈有效显效无效人数及总有效率(%)
痊愈 | 显效 | 有效 | 无效 | 总有效率 | |
第一组 | 23 | 18 | 8 | 1 | 98% |
第二组 | 22 | 16 | 11 | 1 | 98% |
第三组 | 22 | 14 | 13 | 1 | 98% |
第四组 | 2 | 2 | 3 | 43 | 14% |
第五组 | 0 | 0 | 1 | 49 | 2% |
由表1中可以看出,实施例1-3制备的益生菌组合制剂具有优异的治疗便秘的功能,其中,实施例1的治疗效果最好,对临床常见的各种便秘具有很好的治疗效果,其总有效率达98%,对比例1治疗便秘的有效率较低,其原料缺少龙根菌、LA-5嗜酸乳杆菌和BB-12雷特氏B菌,导致益生菌组合制剂治疗便秘的效果不明显,因此本发明益生菌组合制剂的原料组合是特定的,在本发明配方中是缺一不可的。
对于本领域技术人员而言,显然本发明不限于上述示范性实施例的细节,而且在不背离本发明的精神或基本特征的情况下,能够以其他的具体形式实现本发明。因此,无论从哪一点来看,均应将实施例看作是示范性的,而且是非限制性的,本发明的范围由所附权利要求而不是上述说明限定,因此旨在将落在权利要求的等同要件的含义和范围内的所有变化囊括在本发明内。
此外,应当理解,虽然本说明书按照实施方式加以描述,但并非每个实施方式仅包含一个独立的技术方案,说明书的这种叙述方式仅仅是为清楚起见,本领域技术人员应当将说明书作为一个整体,各实施例中的技术方案也可以经适当组合,形成本领域技术人员可以理解的其他实施方式。
Claims (9)
1.一种益生菌组合制剂,其特征在于,其由下述重量份的原料制得:鼠李糖乳杆菌5-15份,副凯氏乳酸杆菌8-16份,植物乳杆菌2-7份,发酵乳酸杆菌7-15份,瑞士乳杆菌1-8份,发酵乳杆菌10-15份,干酪乳杆菌8-17份,乳酸乳球菌12-16份,唾液乳酸杆菌3-8份,龙根菌7-11份,LA-5嗜酸乳杆菌3-15份,BB-12雷特氏B菌7-15份,硅酸钙5-8份,甘油4-10份,阿拉伯胶4-8份,壳聚糖1-5份。
2.根据权利要求1所述一种益生菌组合制剂,其特征在于,其由下述重量份的原料制得:鼠李糖乳杆菌8份,副凯氏乳酸杆菌10份,植物乳杆菌4份,发酵乳酸杆菌11份,瑞士乳杆菌5份,发酵乳杆菌13份,干酪乳杆菌12份,乳酸乳球菌14份,唾液乳酸杆菌5份,龙根菌7份,LA-5嗜酸乳杆菌8份,BB-12雷特氏B菌11份,硅酸钙6份,甘油4份,阿拉伯胶6份,壳聚糖3份。
3.一种益生菌组合制剂的制备方法,其特征在于,包括以下步骤:
(1)按权利要求1或2所述的重量份称取各原料;
(2)将鼠李糖乳杆菌、副凯氏乳酸杆菌、植物乳杆菌、发酵乳酸杆菌、瑞士乳杆菌、发酵乳杆菌、干酪乳杆菌、乳酸乳球菌、唾液乳酸杆菌、龙根菌、LA-5嗜酸乳杆菌、BB-12雷特氏B菌用发酵罐进行混合培养,获得菌泥,备用;
(3)将硅酸钙、甘油、阿拉伯胶、壳聚糖混合搅拌均匀,加入步骤(1)获得的菌泥,均质,经低温冷冻干燥,得益生菌组合制剂;
(4)将步骤(3)得到的益生菌组合制剂用铝箔或棕色玻璃瓶包装。
4.根据权利要求3所述的一种益生菌组合制剂的制备方法,其特征在于,所述步骤(2)中,培养温度为35-37℃,培养时间为12-48h。
5.根据权利要求3所述的一种益生菌组合制剂的制备方法,其特征在于,所述步骤(2)中,获得菌泥中菌体总浓度为1.5×109-1.5×1012CFU/ml。
6.根据权利要求3所述的一种益生菌组合制剂的制备方法,其特征在于,所述步骤(3)中,将硅酸钙、甘油、阿拉伯胶、壳聚糖混合,搅拌时间为20-30分钟。
7.根据权利要求3所述的一种益生菌组合制剂的制备方法,其特征在于,所述步骤(3)中,加入菌泥后,均质时间为40-50分钟。
8.根据权利要求3所述的一种益生菌组合制剂的制备方法,其特征在于,所述步骤(3)中,干燥至含水量为1%-3%。
9.一种权利要求1-3任一项所述的益生菌组合制剂在食品工业、制药工业、健康保健工业或天然产品生产中的应用。
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